Kendal Scot Broadie, Ph.D.


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Faculty Appointments
Stevenson Professor of Neurobiology Stevenson Professor of NeurobiologyProfessor of Cell and Developmental BiologyProfessor of Pharmacology
Ph.D., Zoology, University of Cambridge, Cambridge, United KingdomB.S., University of Oregon, Eugene, Oregon
Office Address
Box 1634 Sta B
Nashville, TN 37232-8548
Research Description
What are the genetic and molecular mechanisms underlying coordinated movement, integrated behavior, cognition, learning and memory? How does the nervous system circuitry underlying these behaviors develop? How is information relayed rapidly and accurately within neural circuits? How are neural circuits modified by experience to optimize behavior? How do these mechanisms go awry in inherited neurological diseases and age-related neurological decline? These questions center around the common themes of information transfer and information storage in cells of the nervous system. My long-term interest has been to understand the fundamental principles of nervous system development, function and use-dependent plasticity by applying systematic genetic analyses to address these questions.

The primary focus of my laboratory is on the synapse, the specialized intercellular junction which functions as the communication link between neurons, and between neurons and muscle. Chemical synapses mediate the vast majority of communication in the nervous system and exhibit plastic properties underlying the behavioral and cognitive malleability of the brain. Our experimental approach is to use a combination of forward genetics, reverse genetics and functional genomics to identify synaptic genes, generate mutants and then assay mutant phenotypes to elucidate the function of normal synaptic gene products. Our laboratory uses this strategy to investigate three closely related questions: 1) How do synapses develop?, 2) How do synapses function? and 3) How do synapses maintain adaptive plasticity?

Synaptic development involves specifying and constructing the intercellular communication link. The developmental program specifies synaptic partnerships and aligns presynaptic signal release with postsynaptic receptors. The mature synapse translates electrical information into chemical information and back again. This conversion requires mechanisms coupling action potential to vesicle fusion, receptor activation and downstream signaling. Synaptic plasticity is the key to nervous system adaptability, including higher cognitive functions. Synaptic structure and function by use, to modulate communication either transiently (learning) or permanently (memory). We screen directly for genetic mutants defective in learning/memory and then assay the roles of the mutant genes at the synapse.

A primary interest is to develop models of human neurological diseases linked to inherited synaptic dysfunction. We study disease states ranging from movement disorders to intellectual disabilities. One focus is Fragile X syndrome (FXS), the most common heritable disease resulting in cognitive impairment and autism spectrum disorder (ASD). Multiple lines of evidence show that FXS is caused by defects in synaptic development and plasticity due to inappropriate translational regulation. We are employing a range of complementary behavioral, electrophysiological, imaging and molecular genetic approaches to study this disease state and test treatments. We are particularly interested in activity-dependent mechanisms during the critical period of synaptic development in central brain circuits.

Our approach to the neuronal synapse is multi-disciplinary and requires the marriage of many traditionally distinct fields. Our strategy involves classic geneticists, molecular biologists, developmental cell biologists, electrophysiologists and behavior scientists. Most members of our research group combine two or more of these approaches in their studies, and also collaborate with others having their own specialized skills. In the long term, we hope that our work will lead to greatly improved understanding of nervous system development, function and plasticity including the molecular mechanisms of neural circuit formation, integrated neural communication, higher brain functions such as learning and memory, and treatments for synaptic dysfunction arising in inherited neurological disease states.
Research Keywords
neurobiology, genetics, genomics, development, synaptogenesis, neurotransmission, synaptic plasticity, behavior, learning & memory, genetic models of human disease. TECHNIQUES: classic and molecular Drosophila genetics, biochemistry, electrophysiology, optical imaging, electron microscopy
Clinical Research Keywords
Developmental Delay (DD), Intellectual Disability (ID), Autism Spectrum Disorder (ASD), Fragile X Syndrome (FXS), Congenital Disorders of Glycosylation (CDG), Muscular Dystrophy (MD), Neurodegeneration (ND)
Parkinson WM, Dookwah M, Dear ML, Gatto CL, Aoki K, Tiemeyer M, Broadie K. Synaptic roles for phosphomannomutase type 2 in a new Drosophila congenital disorder of glycosylation disease model. Dis Model Mech [print-electronic]. 2016 May 5/1/2016; 9(5): 513-27. PMID: 26940433, PMCID: PMC4892659, PII: dmm.022939, DOI: 10.1242/dmm.022939, ISSN: 1754-8411.

Doll CA, Broadie K. Neuron class-specific requirements for Fragile X Mental Retardation Protein in critical period development of calcium signaling in learning and memory circuitry. Neurobiol. Dis [print-electronic]. 2016 May; 89: 76-87. PMID: 26851502, PMCID: PMC4785039, PII: S0969-9961(16)30027-4, DOI: 10.1016/j.nbd.2016.02.006, ISSN: 1095-953X.

Jiang H, Hanna E, Gatto CL, Page TL, Bhuva B, Broadie K. A fully automated Drosophila olfactory classical conditioning and testing system for behavioral learning and memory assessment. J. Neurosci. Methods [print-electronic]. 2016 Mar 3/1/2016; 261: 62-74. PMID: 26703418, PMCID: PMC4749449, PII: S0165-0270(15)00434-3, DOI: 10.1016/j.jneumeth.2015.11.030, ISSN: 1872-678X.

Dear ML, Dani N, Parkinson W, Zhou S, Broadie K. Two classes of matrix metalloproteinases reciprocally regulate synaptogenesis. Development [print-electronic]. 2016 Jan 1/1/2016; 143(1): 75-87. PMID: 26603384, PMCID: PMC4725201, PII: dev.124461, DOI: 10.1242/dev.124461, ISSN: 1477-9129.

Doll CA, Broadie K. Activity-dependent FMRP requirements in development of the neural circuitry of learning and memory. Development. 2015 Apr 4/1/2015; 142(7): 1346-56. PMID: 25804740, PMCID: PMC4378248, PII: 142/7/1346, DOI: 10.1242/dev.117127, ISSN: 1477-9129.

Jumbo-Lucioni P, Parkinson W, Broadie K. Overelaborated synaptic architecture and reduced synaptomatrix glycosylation in a Drosophila classic galactosemia disease model. Dis Model Mech [print-electronic]. 2014 Dec; 7(12): 1365-78. PMID: 25326312, PMCID: PMC4257005, PII: dmm.017137, DOI: 10.1242/dmm.017137, ISSN: 1754-8411.

Dani N, Zhu H, Broadie K. Two protein N-acetylgalactosaminyl transferases regulate synaptic plasticity by activity-dependent regulation of integrin signaling. J. Neurosci. 2014 Sep 9/24/2014; 34(39): 13047-65. PMID: 25253852, PMCID: PMC4172800, PII: 34/39/13047, DOI: 10.1523/JNEUROSCI.1484-14.2014, ISSN: 1529-2401.

Gatto CL, Pereira D, Broadie K. GABAergic circuit dysfunction in the Drosophila Fragile X syndrome model. Neurobiol. Dis [print-electronic]. 2014 May; 65: 142-59. PMID: 24423648, PMCID: PMC3988906, PII: S0969-9961(14)00015-1, DOI: 10.1016/j.nbd.2014.01.008, ISSN: 1095-953X.

Doll CA, Broadie K. Impaired activity-dependent neural circuit assembly and refinement in autism spectrum disorder genetic models. Front Cell Neurosci. 2014; 8: 30. PMID: 24570656, PMCID: PMC3916725, DOI: 10.3389/fncel.2014.00030, ISSN: 1662-5102.

Parkinson W, Dear ML, Rushton E, Broadie K. N-glycosylation requirements in neuromuscular synaptogenesis. Development [print-electronic]. 2013 Dec; 140(24): 4970-81. PMID: 24227656, PMCID: PMC3848190, PII: dev.099192, DOI: 10.1242/dev.099192, ISSN: 1477-9129.

Friedman SH, Dani N, Rushton E, Broadie K. Fragile X mental retardation protein regulates trans-synaptic signaling in Drosophila. Dis Model Mech [print-electronic]. 2013 Nov; 6(6): 1400-13. PMID: 24046358, PMCID: PMC3820263, PII: dmm.012229, DOI: 10.1242/dmm.012229, ISSN: 1754-8411.

Guss KA, Benson M, Gubitosi N, Brondell K, Broadie K, Skeath JB. Expression and function of scalloped during Drosophila development. Dev. Dyn [print-electronic]. 2013 Jul; 242(7): 874-85. PMID: 23389965, PMCID: PMC3887039, DOI: 10.1002/dvdy.23942, ISSN: 1097-0177.

Staples J, Broadie K. The cell polarity scaffold Lethal Giant Larvae regulates synapse morphology and function. J. Cell. Sci [print-electronic]. 2013 May 5/1/2013; 126(Pt 9): 1992-2003. PMID: 23444371, PMCID: PMC3666253, PII: jcs.120139, DOI: 10.1242/jcs.120139, ISSN: 1477-9137.

Rohrbough J, Kent KS, Broadie K, Weiss JB. Jelly Belly trans-synaptic signaling to anaplastic lymphoma kinase regulates neurotransmission strength and synapse architecture. Dev Neurobiol [print-electronic]. 2013 Mar; 73(3): 189-208. PMID: 22949158, PMCID: PMC3565053, DOI: 10.1002/dneu.22056, ISSN: 1932-846X.

Nahm M, Lee MJ, Parkinson W, Lee M, Kim H, Kim YJ, Kim S, Cho YS, Min BM, Bae YC, Broadie K, Lee S. Spartin regulates synaptic growth and neuronal survival by inhibiting BMP-mediated microtubule stabilization. Neuron. 2013 Feb 2/20/2013; 77(4): 680-95. PMID: 23439121, PMCID: PMC3815429, PII: S0896-6273(12)01125-7, DOI: 10.1016/j.neuron.2012.12.015, ISSN: 1097-4199.

Wei C, Thatcher EJ, Olena AF, Cha DJ, Perdigoto AL, Marshall AF, Carter BD, Broadie K, Patton JG. MiR-153 regulates SNAP-25, synaptic transmission, and neuronal development. PLoS ONE [print-electronic]. 2013; 8(2): e57080. PMID: 23451149, PMCID: PMC3581580, PII: PONE-D-12-37460, DOI: 10.1371/journal.pone.0057080, ISSN: 1932-6203.

Rushton E, Rohrbough J, Deutsch K, Broadie K. Structure-function analysis of endogenous lectin mind-the-gap in synaptogenesis. Dev Neurobiol [print-electronic]. 2012 Aug; 72(8): 1161-79. PMID: 22234957, PMCID: PMC3417755, DOI: 10.1002/dneu.22006, ISSN: 1932-846X.

Coffee RL, Williamson AJ, Adkins CM, Gray MC, Page TL, Broadie K. In vivo neuronal function of the fragile X mental retardation protein is regulated by phosphorylation. Hum. Mol. Genet [print-electronic]. 2012 Feb 2/15/2012; 21(4): 900-15. PMID: 22080836, PMCID: PMC3263990, PII: ddr527, DOI: 10.1093/hmg/ddr527, ISSN: 1460-2083.

Dani N, Broadie K. Glycosylated synaptomatrix regulation of trans-synaptic signaling. Dev Neurobiol. 2012 Jan; 72(1): 2-21. PMID: 21509945, PMCID: PMC3240703, DOI: 10.1002/dneu.20891, ISSN: 1932-846X.

Dani N, Nahm M, Lee S, Broadie K. A targeted glycan-related gene screen reveals heparan sulfate proteoglycan sulfation regulates WNT and BMP trans-synaptic signaling. PLoS Genet [print-electronic]. 2012; 8(11): e1003031. PMID: 23144627, PMCID: PMC3493450, PII: PGENETICS-D-12-01154, DOI: 10.1371/journal.pgen.1003031, ISSN: 1553-7404.

Siller SS, Broadie K. Matrix metalloproteinases and minocycline: therapeutic avenues for fragile X syndrome. Neural Plast [print-electronic]. 2012; 2012: 124548. PMID: 22685676, PMCID: PMC3364018, DOI: 10.1155/2012/124548, ISSN: 1687-5443.

Tessier CR, Broadie K. Molecular and genetic analysis of the Drosophila model of fragile X syndrome. Results Probl Cell Differ. 2012; 54: 119-56. PMID: 22009350, DOI: 10.1007/978-3-642-21649-7_7, ISSN: 0080-1844.

Gatto CL, Broadie K. Drosophila modeling of heritable neurodevelopmental disorders. Curr. Opin. Neurobiol [print-electronic]. 2011 Dec; 21(6): 834-41. PMID: 21596554, PMCID: PMC3172335, PII: S0959-4388(11)00067-5, DOI: 10.1016/j.conb.2011.04.009, ISSN: 1873-6882.

Broadie K, Baumgartner S, Prokop A. Extracellular matrix and its receptors in Drosophila neural development. Dev Neurobiol. 2011 Nov; 71(11): 1102-30. PMID: 21688401, PMCID: PMC3192297, DOI: 10.1002/dneu.20935, ISSN: 1932-846X.

Siller SS, Broadie K. Neural circuit architecture defects in a Drosophila model of Fragile X syndrome are alleviated by minocycline treatment and genetic removal of matrix metalloproteinase. Dis Model Mech [print-electronic]. 2011 Sep; 4(5): 673-85. PMID: 21669931, PMCID: PMC3180232, PII: dmm.008045, DOI: 10.1242/dmm.008045, ISSN: 1754-8411.

Gatto CL, Broadie K. Fragile X mental retardation protein is required for programmed cell death and clearance of developmentally-transient peptidergic neurons. Dev. Biol [print-electronic]. 2011 Aug 8/15/2011; 356(2): 291-307. PMID: 21596027, PMCID: PMC3143227, PII: S0012-1606(11)00298-3, DOI: 10.1016/j.ydbio.2011.05.001, ISSN: 1095-564X.

Tessier CR, Broadie K. The fragile X mental retardation protein developmentally regulates the strength and fidelity of calcium signaling in Drosophila mushroom body neurons. Neurobiol. Dis [print-electronic]. 2011 Jan; 41(1): 147-59. PMID: 20843478, PMCID: PMC2982942, PII: S0969-9961(10)00303-7, DOI: 10.1016/j.nbd.2010.09.002, ISSN: 1095-953X.

Vijayakrishnan N, Phillips SE, Broadie K. Drosophila rolling blackout displays lipase domain-dependent and -independent endocytic functions downstream of dynamin. Traffic [print-electronic]. 2010 Dec; 11(12): 1567-78. PMID: 21029287, PMCID: PMC2975403, DOI: 10.1111/j.1600-0854.2010.01117.x, ISSN: 1600-0854.

Nahm M, Long AA, Paik SK, Kim S, Bae YC, Broadie K, Lee S. The Cdc42-selective GAP rich regulates postsynaptic development and retrograde BMP transsynaptic signaling. J. Cell Biol. 2010 Nov 11/1/2010; 191(3): 661-75. PMID: 21041451, PMCID: PMC3003324, PII: jcb.201007086, DOI: 10.1083/jcb.201007086, ISSN: 1540-8140.

Rohrbough J, Broadie K. Anterograde Jelly belly ligand to Alk receptor signaling at developing synapses is regulated by Mind the gap. Development. 2010 Oct; 137(20): 3523-33. PMID: 20876658, PMCID: PMC2947762, PII: 137/20/3523, DOI: 10.1242/dev.047878, ISSN: 1477-9129.

Long AA, Mahapatra CT, Woodruff EA, Rohrbough J, Leung HT, Shino S, An L, Doerge RW, Metzstein MM, Pak WL, Broadie K. The nonsense-mediated decay pathway maintains synapse architecture and synaptic vesicle cycle efficacy. J. Cell. Sci [print-electronic]. 2010 Oct 10/1/2010; 123(Pt 19): 3303-15. PMID: 20826458, PMCID: PMC2939802, PII: jcs.069468, DOI: 10.1242/jcs.069468, ISSN: 1477-9137.

Coffee RL, Tessier CR, Woodruff EA, Broadie K. Fragile X mental retardation protein has a unique, evolutionarily conserved neuronal function not shared with FXR1P or FXR2P. Dis Model Mech [print-electronic]. 2010 Jul; 3(7-8): 471-85. PMID: 20442204, PMCID: PMC2898537, PII: dmm.004598, DOI: 10.1242/dmm.004598, ISSN: 1754-8411.

Kliman M, Vijayakrishnan N, Wang L, Tapp JT, Broadie K, McLean JA. Structural mass spectrometry analysis of lipid changes in a Drosophila epilepsy model brain. Mol Biosyst [print-electronic]. 2010 Jun; 6(6): 958-66. PMID: 20379606, PMCID: PMC3848785, DOI: 10.1039/b927494d, ISSN: 1742-2051.

Gatto CL, Broadie K. Genetic controls balancing excitatory and inhibitory synaptogenesis in neurodevelopmental disorder models. Front Synaptic Neurosci. 2010; 2: 4. PMID: 21423490, PMCID: PMC3059704, DOI: 10.3389/fnsyn.2010.00004, ISSN: 1663-3563.

Gatto CL, Broadie K. The fragile X mental retardation protein in circadian rhythmicity and memory consolidation. Mol. Neurobiol [print-electronic]. 2009 Apr; 39(2): 107-29. PMID: 19214804, PMCID: PMC3848790, DOI: 10.1007/s12035-009-8057-0, ISSN: 0893-7648.

Rushton E, Rohrbough J, Broadie K. Presynaptic secretion of mind-the-gap organizes the synaptic extracellular matrix-integrin interface and postsynaptic environments. Dev. Dyn. 2009 Mar; 238(3): 554-71. PMID: 19235718, PMCID: PMC2677818, DOI: 10.1002/dvdy.21864, ISSN: 1058-8388.

Repicky S, Broadie K. Metabotropic glutamate receptor-mediated use-dependent down-regulation of synaptic excitability involves the fragile X mental retardation protein. J. Neurophysiol [print-electronic]. 2009 Feb; 101(2): 672-87. PMID: 19036865, PMCID: PMC2657068, PII: 90953.2008, DOI: 10.1152/jn.90953.2008, ISSN: 0022-3077.

Gatto CL, Broadie K. Temporal requirements of the fragile x mental retardation protein in modulating circadian clock circuit synaptic architecture. Front Neural Circuits. 2009; 3: 8. PMID: 19738924, PMCID: PMC2737437, DOI: 10.3389/neuro.04.008.2009, ISSN: 1662-5110.

Tessier CR, Broadie K. Activity-dependent modulation of neural circuit synaptic connectivity. Front Mol Neurosci. 2009; 2: 8. PMID: 19668708, PMCID: PMC2724028, DOI: 10.3389/neuro.02.008.2009, ISSN: 1662-5099.

Featherstone DE, Chen K, Broadie K. Harvesting and preparing Drosophila embryos for electrophysiological recording and other procedures. J Vis Exp. 2009; (27): PMID: 19488027, PMCID: PMC2794686, PII: 1347, DOI: 10.3791/1347, ISSN: 1940-087X.

Chen K, Featherstone DE, Broadie K. Electrophysiological recording in the Drosophila embryo. J Vis Exp. 2009; (27): PMID: 19461578, PMCID: PMC2794687, PII: 1348, DOI: 10.3791/1348, ISSN: 1940-087X.

Vijayakrishnan N, Woodruff EA, Broadie K. Rolling blackout is required for bulk endocytosis in non-neuronal cells and neuronal synapses. J. Cell. Sci [print-electronic]. 2009 Jan 1/1/2009; 122(Pt 1): 114-25. PMID: 19066280, PMCID: PMC2714403, PII: jcs.036673, DOI: 10.1242/jcs.036673, ISSN: 0021-9533.

Woodruff EA, Broadie K, Honegger HW. Two peptide transmitters co-packaged in a single neurosecretory vesicle. Peptides [print-electronic]. 2008 Dec; 29(12): 2276-80. PMID: 18848852, PMCID: PMC2637405, PII: S0196-9781(08)00370-7, DOI: 10.1016/j.peptides.2008.08.023, ISSN: 0196-9781.

Venkatachalam K, Long AA, Elsaesser R, Nikolaeva D, Broadie K, Montell C. Motor deficit in a Drosophila model of mucolipidosis type IV due to defective clearance of apoptotic cells. Cell. 2008 Nov 11/28/2008; 135(5): 838-51. PMID: 19041749, PMCID: PMC2649760, PII: S0092-8674(08)01198-7, DOI: 10.1016/j.cell.2008.09.041, ISSN: 1097-4172.

Bolduc FV, Bell K, Cox H, Broadie KS, Tully T. Excess protein synthesis in Drosophila fragile X mutants impairs long-term memory. Nat. Neurosci [print-electronic]. 2008 Oct; 11(10): 1143-5. PMID: 18776892, PMCID: PMC3038669, PII: nn.2175, DOI: 10.1038/nn.2175, ISSN: 1546-1726.

Gatto CL, Broadie K. Temporal requirements of the fragile X mental retardation protein in the regulation of synaptic structure. Development [print-electronic]. 2008 Aug; 135(15): 2637-48. PMID: 18579676, PMCID: PMC2753511, PII: dev.022244, DOI: 10.1242/dev.022244, ISSN: 0950-1991.

Haas KF, Broadie K. Roles of ubiquitination at the synapse. Biochim. Biophys. Acta [print-electronic]. 2008 Aug; 1779(8): 495-506. PMID: 18222124, PMCID: PMC2668815, PII: S1874-9399(07)00203-9, DOI: 10.1016/j.bbagrm.2007.12.010, ISSN: 0006-3002.

Phillips SE, Woodruff EA, Liang P, Patten M, Broadie K. Neuronal loss of Drosophila NPC1a causes cholesterol aggregation and age-progressive neurodegeneration. J. Neurosci. 2008 Jun 6/25/2008; 28(26): 6569-82. PMID: 18579730, PMCID: PMC3306184, PII: 28/26/6569, DOI: 10.1523/JNEUROSCI.5529-07.2008, ISSN: 1529-2401.

Mohrmann R, Matthies HJ, Woodruff E, Broadie K. Stoned B mediates sorting of integral synaptic vesicle proteins. Neuroscience [print-electronic]. 2008 Jun 6/2/2008; 153(4): 1048-63. PMID: 18436388, PMCID: PMC2696304, PII: S0306-4522(08)00253-4, DOI: 10.1016/j.neuroscience.2008.02.060, ISSN: 0306-4522.

Long AA, Kim E, Leung HT, Woodruff E, An L, Doerge RW, Pak WL, Broadie K. Presynaptic calcium channel localization and calcium-dependent synaptic vesicle exocytosis regulated by the Fuseless protein. J. Neurosci. 2008 Apr 4/2/2008; 28(14): 3668-82. PMID: 18385325, PMCID: PMC2769928, PII: 28/14/3668, DOI: 10.1523/JNEUROSCI.5553-07.2008, ISSN: 1529-2401.

Tessier CR, Broadie K. Drosophila fragile X mental retardation protein developmentally regulates activity-dependent axon pruning. Development [print-electronic]. 2008 Apr; 135(8): 1547-57. PMID: 18321984, PMCID: PMC3988902, PII: dev.015867, DOI: 10.1242/dev.015867, ISSN: 0950-1991.

Pan L, Woodruff E, Liang P, Broadie K. Mechanistic relationships between Drosophila fragile X mental retardation protein and metabotropic glutamate receptor A signaling. Mol. Cell. Neurosci [print-electronic]. 2008 Apr; 37(4): 747-60. PMID: 18280750, PMCID: PMC3989938, PII: S1044-7431(08)00004-3, DOI: 10.1016/j.mcn.2008.01.003, ISSN: 1095-9327.

Reeve SP, Lin X, Sahin BH, Jiang F, Yao A, Liu Z, Zhi H, Broadie K, Li W, Giangrande A, Hassan BA, Zhang YQ. Mutational analysis establishes a critical role for the N terminus of fragile X mental retardation protein FMRP. J. Neurosci. 2008 Mar 3/19/2008; 28(12): 3221-6. PMID: 18354025, PII: 28/12/3221, DOI: 10.1523/JNEUROSCI.5528-07.2008, ISSN: 1529-2401.

Kucenas S, Takada N, Park HC, Woodruff E, Broadie K, Appel B. CNS-derived glia ensheath peripheral nerves and mediate motor root development. Nat. Neurosci [print-electronic]. 2008 Feb; 11(2): 143-51. PMID: 18176560, PMCID: PMC2657597, PII: nn2025, DOI: 10.1038/nn2025, ISSN: 1097-6256.

Pan L, Broadie KS. Drosophila fragile X mental retardation protein and metabotropic glutamate receptor A convergently regulate the synaptic ratio of ionotropic glutamate receptor subclasses. J. Neurosci. 2007 Nov 11/7/2007; 27(45): 12378-89. PMID: 17989302, PII: 27/45/12378, DOI: 10.1523/JNEUROSCI.2970-07.2007, ISSN: 1529-2401.

Haas KF, Woodruff E, Broadie K. Proteasome function is required to maintain muscle cellular architecture. Biol. Cell. 2007 Nov; 99(11): 615-26. PMID: 17523916, PMCID: PMC2712885, PII: BC20070019, DOI: 10.1042/BC20070019, ISSN: 1768-322X.

Rohrbough J, Rushton E, Woodruff E, Fergestad T, Vigneswaran K, Broadie K. Presynaptic establishment of the synaptic cleft extracellular matrix is required for post-synaptic differentiation. Genes Dev [print-electronic]. 2007 Oct 10/15/2007; 21(20): 2607-28. PMID: 17901219, PMCID: PMC2000325, PII: gad.1574107, DOI: 10.1101/gad.1574107, ISSN: 0890-9369.

Yan Y, Broadie K. In vivo assay of presynaptic microtubule cytoskeleton dynamics in Drosophila. J. Neurosci. Methods [print-electronic]. 2007 May 5/15/2007; 162(1-2): 198-205. PMID: 17331586, PMCID: PMC2713775, PII: S0165-0270(07)00032-5, DOI: 10.1016/j.jneumeth.2007.01.013, ISSN: 0165-0270.

Haas KF, Miller SL, Friedman DB, Broadie K. The ubiquitin-proteasome system postsynaptically regulates glutamatergic synaptic function. Mol. Cell. Neurosci [print-electronic]. 2007 May; 35(1): 64-75. PMID: 17363264, PMCID: PMC1936977, PII: S1044-7431(07)00027-9, DOI: 10.1016/j.mcn.2007.02.002, ISSN: 1044-7431.

Huang FD, Woodruff E, Mohrmann R, Broadie K. Rolling blackout is required for synaptic vesicle exocytosis. J. Neurosci. 2006 Mar 3/1/2006; 26(9): 2369-79. PMID: 16510714, PII: 26/9/2369, DOI: 10.1523/JNEUROSCI.3770-05.2006, ISSN: 1529-2401.

Vijayakrishnan N, Broadie K. Temperature-sensitive paralytic mutants: insights into the synaptic vesicle cycle. Biochem. Soc. Trans. 2006 Feb; 34(Pt 1): 81-7. PMID: 16417488, PII: BST0340081, DOI: 10.1042/BST0340081, ISSN: 0300-5127.

Huang, F.D., Woodruff, E., Mohrmann, R. and Broadie, K.. Rolling Blackout is acutely required for synaptic vesicle exocytosis. Journal of Neuroscience. 2006; 26: 2369-79.

Featherstone DE, Rushton E, Rohrbough J, Liebl F, Karr J, Sheng Q, Rodesch CK, Broadie K. An essential Drosophila glutamate receptor subunit that functions in both central neuropil and neuromuscular junction. J. Neurosci. 2005 Mar 3/23/2005; 25(12): 3199-208. PMID: 15788777, PMCID: PMC2194804, PII: 25/12/3199, DOI: 10.1523/JNEUROSCI.4201-04.2005, ISSN: 1529-2401.

Broadie K, Pan L. Translational complexity of the fragile x mental retardation protein: insights from the fly. Mol. Cell. 2005 Mar 3/18/2005; 17(6): 757-9. PMID: 15780932, PII: S1097-2765(05)01155-X, DOI: 10.1016/j.molcel.2005.03.004, ISSN: 1097-2765.

Zhang YQ, Friedman DB, Wang Z, Woodruff E, Pan L, O'donnell J, Broadie K. Protein expression profiling of the drosophila fragile X mutant brain reveals up-regulation of monoamine synthesis. Mol. Cell Proteomics [print-electronic]. 2005 Mar; 4(3): 278-90. PMID: 15634690, PII: M400174-MCP200, DOI: 10.1074/mcp.M400174-MCP200, ISSN: 1535-9476.

Rohrbough J, Broadie K. Lipid regulation of the synaptic vesicle cycle. Nat. Rev. Neurosci. 2005 Feb; 6(2): 139-50. PMID: 15685219, PII: nrn1608, DOI: 10.1038/nrn1608, ISSN: 1471-003X.

Zhang YQ, Broadie K. Fathoming fragile X in fruit flies. Trends Genet. 2005 Jan; 21(1): 37-45. PMID: 15680513, PII: S0168-9525(04)00305-1, DOI: 10.1016/j.tig.2004.11.003, ISSN: 0168-9525.

Pan L, Zhang YQ, Woodruff E, Broadie K. The Drosophila fragile X gene negatively regulates neuronal elaboration and synaptic differentiation. Curr. Biol. 2004 Oct 10/26/2004; 14(20): 1863-70. PMID: 15498496, PII: S0960982204007560, DOI: 10.1016/j.cub.2004.09.085, ISSN: 0960-9822.

Bogdanik L, Mohrmann R, Ramaekers A, Bockaert J, Grau Y, Broadie K, Parmentier ML. The Drosophila metabotropic glutamate receptor DmGluRA regulates activity-dependent synaptic facilitation and fine synaptic morphology. J. Neurosci. 2004 Oct 10/13/2004; 24(41): 9105-16. PMID: 15483129, PII: 24/41/9105, DOI: 10.1523/JNEUROSCI.2724-04.2004, ISSN: 1529-2401.

Broadie K. Synapse scaffolding: intersection of endocytosis and growth. Curr. Biol. 2004 Oct 10/5/2004; 14(19): R853-5. PMID: 15458667, PII: S0960982204007262, DOI: 10.1016/j.cub.2004.09.042, ISSN: 0960-9822.

Huang FD, Matthies HJ, Speese SD, Smith MA, Broadie K. Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction. Nat. Neurosci [print-electronic]. 2004 Oct; 7(10): 1070-8. PMID: 15361878, PII: nn1313, DOI: 10.1038/nn1313, ISSN: 1097-6256.

Rohrbough J, Rushton E, Palanker L, Woodruff E, Matthies HJ, Acharya U, Acharya JK, Broadie K. Ceramidase regulates synaptic vesicle exocytosis and trafficking. J. Neurosci. 2004 Sep 9/8/2004; 24(36): 7789-803. PMID: 15356190, PMCID: PMC2675194, PII: 24/36/7789, DOI: 10.1523/JNEUROSCI.1146-04.2004, ISSN: 1529-2401.

Trotta N, Rodesch CK, Fergestad T, Broadie K. Cellular bases of activity-dependent paralysis in Drosophila stress-sensitive mutants. J. Neurobiol. 2004 Sep 9/5/2004; 60(3): 328-47. PMID: 15281071, DOI: 10.1002/neu.20017, ISSN: 0022-3034.

Trotta N, Orso G, Rossetto MG, Daga A, Broadie K. The hereditary spastic paraplegia gene, spastin, regulates microtubule stability to modulate synaptic structure and function. Curr. Biol. 2004 Jul 7/13/2004; 14(13): 1135-47. PMID: 15242610, PII: S0960982204004634, DOI: 10.1016/j.cub.2004.06.058, ISSN: 0960-9822.

Zhang YQ, Matthies HJ, Mancuso J, Andrews HK, Woodruff E, Friedman D, Broadie K. The Drosophila fragile X-related gene regulates axoneme differentiation during spermatogenesis. Dev. Biol. 2004 Jun 6/15/2004; 270(2): 290-307. PMID: 15183715, PII: S0012160604001265, DOI: 10.1016/j.ydbio.2004.02.010, ISSN: 0012-1606.

Broadie K. Axon pruning: an active role for glial cells. Curr. Biol. 2004 Apr 4/20/2004; 14(8): R302-4. PMID: 15084298, PII: S096098220400226X, DOI: 10.1016/j.cub.2004.03.049, ISSN: 0960-9822.

Mohrmann, R., Bogdanik, L., Ramaekers, A., Bockaert, J., Grau, Y., Paramentier, M.L.* and Broadie, K.*. The Drosophila metabotrophic glutamate receptor, DmGluRA, regulates activity-dependent synaptic facilitation and fine synaptic morphology. Journal of Neuroscience. 2004; 24: 9105-16.

Speese SD, Trotta N, Rodesch CK, Aravamudan B, Broadie K. The ubiquitin proteasome system acutely regulates presynaptic protein turnover and synaptic efficacy. Curr. Biol. 2003 May 5/27/2003; 13(11): 899-910. PMID: 12781128, PII: S0960982203003385, ISSN: 0960-9822.

Renden RB, Broadie K. Mutation and activation of Galpha s similarly alters pre- and postsynaptic mechanisms modulating neurotransmission. J. Neurophysiol [print-electronic]. 2003 May; 89(5): 2620-38. PMID: 12611964, PII: 01072.2002, DOI: 10.1152/jn.01072.2002, ISSN: 0022-3077.

Aravamudan B, Broadie K. Synaptic Drosophila UNC-13 is regulated by antagonistic G-protein pathways via a proteasome-dependent degradation mechanism. J. Neurobiol. 2003 Feb 2/15/2003; 54(3): 417-38. PMID: 12532395, DOI: 10.1002/neu.10142, ISSN: 0022-3034.

Matthies HJ, Broadie K. Techniques to dissect cellular and subcellular function in the Drosophila nervous system. Methods Cell Biol. 2003; 71: 195-265. PMID: 12884693, ISSN: 0091-679X.

Rohrbough J, O'Dowd DK, Baines RA, Broadie K. Cellular bases of behavioral plasticity: establishing and modifying synaptic circuits in the Drosophila genetic system. J. Neurobiol. 2003 Jan; 54(1): 254-71. PMID: 12486708, DOI: 10.1002/neu.10171, ISSN: 0022-3034.

Andrews HK, Zhang YQ, Trotta N, Broadie K. Drosophila sec10 is required for hormone secretion but not general exocytosis or neurotransmission. Traffic. 2002 Dec; 3(12): 906-21. PMID: 12453153, PII: tra031206, ISSN: 1398-9219.

Richmond JE, Broadie KS. The synaptic vesicle cycle: exocytosis and endocytosis in Drosophila and C. elegans. Curr. Opin. Neurobiol. 2002 Oct; 12(5): 499-507. PMID: 12367628, PII: S0959438802003604, ISSN: 0959-4388.

Broadie KS, Richmond JE. Establishing and sculpting the synapse in Drosophila and C. elegans. Curr. Opin. Neurobiol. 2002 Oct; 12(5): 491-8. PMID: 12367627, PII: S0959438802003598, ISSN: 0959-4388.

Zhang YQ, Rodesch CK, Broadie K. Living synaptic vesicle marker: synaptotagmin-GFP. Genesis. 2002 Sep; 34(1-2): 142-5. PMID: 12324970, DOI: 10.1002/gene.10144, ISSN: 1526-954X.

Rohrbough J, Broadie K. Electrophysiological analysis of synaptic transmission in central neurons of Drosophila larvae. J. Neurophysiol. 2002 Aug; 88(2): 847-60. PMID: 12163536, ISSN: 0022-3077.

Featherstone D, Broadie K. Response: meaningless minis?. Trends Neurosci. 2002 Aug; 25(8): 386-7. PMID: 12127747, PII: S0166-2236(02)02213-0, ISSN: 0166-2236.

Beumer K, Matthies HJ, Bradshaw A, Broadie K. Integrins regulate DLG/FAS2 via a CaM kinase II-dependent pathway to mediate synapse elaboration and stabilization during postembryonic development. Development. 2002 Jul; 129(14): 3381-91. PMID: 12091308, ISSN: 0950-1991.

Featherstone DE, Broadie K. Wrestling with pleiotropy: genomic and topological analysis of the yeast gene expression network. Bioessays. 2002 Mar; 24(3): 267-74. PMID: 11891763, PII: 10.1002/bies.10054, DOI: 10.1002/bies.10054, ISSN: 0265-9247.

Featherstone DE, Rushton E, Broadie K. Developmental regulation of glutamate receptor field size by nonvesicular glutamate release. Nat. Neurosci. 2002 Feb; 5(2): 141-6. PMID: 11753421, PII: nn789, DOI: 10.1038/nn789, ISSN: 1097-6256.

Fergestad T, Wu MN, Schulze KL, Lloyd TE, Bellen HJ, Broadie K. Targeted mutations in the syntaxin H3 domain specifically disrupt SNARE complex function in synaptic transmission. J. Neurosci. 2001 Dec 12/1/2001; 21(23): 9142-50. PMID: 11717347, PII: 21/23/9142, ISSN: 1529-2401.

Zhang YQ, Bailey AM, Matthies HJ, Renden RB, Smith MA, Speese SD, Rubin GM, Broadie K. Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function. Cell. 2001 Nov 11/30/2001; 107(5): 591-603. PMID: 11733059, PII: S0092-8674(01)00589-X, ISSN: 0092-8674.

Renden R, Berwin B, Davis W, Ann K, Chin CT, Kreber R, Ganetzky B, Martin TF, Broadie K. Drosophila CAPS is an essential gene that regulates dense-core vesicle release and synaptic vesicle fusion. Neuron. 2001 Aug 8/16/2001; 31(3): 421-37. PMID: 11516399, PII: S0896-6273(01)00382-8, ISSN: 0896-6273.

Featherstone DE, Davis WS, Dubreuil RR, Broadie K. Drosophila alpha- and beta-spectrin mutations disrupt presynaptic neurotransmitter release. J. Neurosci. 2001 Jun 6/15/2001; 21(12): 4215-24. PMID: 11404407, PII: 21/12/4215, ISSN: 1529-2401.

Bodily KD, Morrison CM, Renden RB, Broadie K. A novel member of the Ig superfamily, turtle, is a CNS-specific protein required for coordinated motor control. J. Neurosci. 2001 May 5/1/2001; 21(9): 3113-25. PMID: 11312296, PII: 21/9/3113, ISSN: 1529-2401.

Fergestad T, Broadie K. Interaction of stoned and synaptotagmin in synaptic vesicle endocytosis. J. Neurosci. 2001 Feb 2/15/2001; 21(4): 1218-27. PMID: 11160392, PII: 21/4/1218, ISSN: 1529-2401.

Rodesch CK, Broadie K. Genetic studies in Drosophila: vesicle pools and cytoskeleton-based regulation of synaptic transmission. Neuroreport. 2000 Dec 12/18/2000; 11(18): R45-53. PMID: 11192639, ISSN: 0959-4965.

Featherstone DE, Broadie K. Surprises from Drosophila: genetic mechanisms of synaptic development and plasticity. Brain Res. Bull. 2000 Nov 11/15/2000; 53(5): 501-11. PMID: 11165785, PII: S0361-9230(00)00383-X, ISSN: 0361-9230.

Rohrbough J, Grotewiel MS, Davis RL, Broadie K. Integrin-mediated regulation of synaptic morphology, transmission, and plasticity. J. Neurosci. 2000 Sep 9/15/2000; 20(18): 6868-78. PMID: 10995831, PII: 20/18/6868, ISSN: 0270-6474.

Zhang Y, Featherstone D, Davis W, Rushton E, Broadie K. Drosophila D-titin is required for myoblast fusion and skeletal muscle striation. J. Cell. Sci. 2000 Sep; 113 ( Pt 17): 3103-15. PMID: 10934048, ISSN: 0021-9533.

Featherstone DE, Rushton EM, Hilderbrand-Chae M, Phillips AM, Jackson FR, Broadie K. Presynaptic glutamic acid decarboxylase is required for induction of the postsynaptic receptor field at a glutamatergic synapse. Neuron. 2000 Jul; 27(1): 71-84. PMID: 10939332, PII: S0896-6273(00)00010-6, ISSN: 0896-6273.

Beumer KJ, Rohrbough J, Prokop A, Broadie K. A role for PS integrins in morphological growth and synaptic function at the postembryonic neuromuscular junction of Drosophila. Development. 1999 Dec; 126(24): 5833-46. PMID: 10572057, ISSN: 0950-1991.

Aravamudan B, Fergestad T, Davis WS, Rodesch CK, Broadie K. Drosophila UNC-13 is essential for synaptic transmission. Nat. Neurosci. 1999 Nov; 2(11): 965-71. PMID: 10526334, DOI: 10.1038/14764, ISSN: 1097-6256.

Fergestad T, Davis WS, Broadie K. The stoned proteins regulate synaptic vesicle recycling in the presynaptic terminal. J. Neurosci. 1999 Jul 7/15/1999; 19(14): 5847-60. PMID: 10407025, ISSN: 1529-2401.

Wu MN, Fergestad T, Lloyd TE, He Y, Broadie K, Bellen HJ. Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo. Neuron. 1999 Jul; 23(3): 593-605. PMID: 10433270, PII: S0896-6273(00)80811-9, ISSN: 0896-6273.

Zhang YQ, Broadie K. Cloning, mapping and tissue-specific expression of Drosophila clathrin-associated protein AP50 gene. Gene. 1999 Jun 6/11/1999; 233(1-2): 171-9. PMID: 10375633, PII: S0378111999001420, ISSN: 0378-1119.

Rohrbough J, Pinto S, Mihalek RM, Tully T, Broadie K. Latheo, a Drosophila gene involved in learning, regulates functional synaptic plasticity. Neuron. 1999 May; 23(1): 55-70. PMID: 10402193, PII: S0896-6273(00)80753-9, ISSN: 0896-6273.

Broadie KS. Development of electrical properties and synaptic transmission at the embryonic neuromuscular junction. Int. Rev. Neurobiol. 1999; 43: 45-67. PMID: 10218154, ISSN: 0074-7742.

Broadie K. Forward and reverse genetic approaches to synaptogenesis. Curr. Opin. Neurobiol. 1998 Feb; 8(1): 128-38. PMID: 9568400, PII: S0959-4388(98)80016-0, ISSN: 0959-4388.

Broadie K, Rushton E, Skoulakis EM, Davis RL. Leonardo, a Drosophila 14-3-3 protein involved in learning, regulates presynaptic function. Neuron. 1997 Aug; 19(2): 391-402. PMID: 9292728, PII: S0896-6273(00)80948-4, ISSN: 0896-6273.

Baumgartner S, Littleton JT, Broadie K, Bhat MA, Harbecke R, Lengyel JA, Chiquet-Ehrismann R, Prokop A, Bellen HJ. A Drosophila neurexin is required for septate junction and blood-nerve barrier formation and function. Cell. 1996 Dec 12/13/1996; 87(6): 1059-68. PMID: 8978610, PII: S0092-8674(00)81800-0, ISSN: 0092-8674.

Prokop A, Landgraf M, Rushton E, Broadie K, Bate M. Presynaptic development at the Drosophila neuromuscular junction: assembly and localization of presynaptic active zones. Neuron. 1996 Oct; 17(4): 617-26. PMID: 8893020, PII: S0896-6273(00)80195-6, ISSN: 0896-6273.

Broadie KS. Regulation of the synaptic vesicle cycle in Drosophila. Biochem. Soc. Trans. 1996 Aug; 24(3): 639-45. PMID: 8878818, ISSN: 0300-5127.

Keshishian H, Broadie K, Chiba A, Bate M. The drosophila neuromuscular junction: a model system for studying synaptic development and function. Annu. Rev. Neurosci. 1996; 19: 545-75. PMID: 8833454, DOI: 10.1146/, ISSN: 0147-006X.

Broadie K, Prokop A, Bellen HJ, O'Kane CJ, Schulze KL, Sweeney ST. Syntaxin and synaptobrevin function downstream of vesicle docking in Drosophila. Neuron. 1995 Sep; 15(3): 663-73. PMID: 7546745, PII: 0896-6273(95)90154-X, ISSN: 0896-6273.

Bate M, Broadie K. Wiring by fly: the neuromuscular system of the Drosophila embryo. Neuron. 1995 Sep; 15(3): 513-25. PMID: 7546732, PII: 0896-6273(95)90141-8, ISSN: 0896-6273.

Auld VJ, Fetter RD, Broadie K, Goodman CS. Gliotactin, a novel transmembrane protein on peripheral glia, is required to form the blood-nerve barrier in Drosophila. Cell. 1995 Jun 6/2/1995; 81(5): 757-67. PMID: 7539719, PII: 0092-8674(95)90537-5, ISSN: 0092-8674.

Sweeney ST, Broadie K, Keane J, Niemann H, O'Kane CJ. Targeted expression of tetanus toxin light chain in Drosophila specifically eliminates synaptic transmission and causes behavioral defects. Neuron. 1995 Feb; 14(2): 341-51. PMID: 7857643, PII: 0896-6273(95)90290-2, ISSN: 0896-6273.

Schulze KL, Broadie K, Perin MS, Bellen HJ. Genetic and electrophysiological studies of Drosophila syntaxin-1A demonstrate its role in nonneuronal secretion and neurotransmission. Cell. 1995 Jan 1/27/1995; 80(2): 311-20. PMID: 7834751, PII: 0092-8674(95)90414-X, ISSN: 0092-8674.

Broadie KS. Genetic dissection of the molecular mechanisms of transmitter vesicle release during synaptic transmission. J. Physiol. Paris. 1995; 89(2): 59-70. PMID: 8520572, PII: 0928-4257(96)80552-3, ISSN: 0928-4257.

Broadie, K., Sweeney, S.T., Keane, J., Niemann, H. and O'Kane, C.J. Targeted expression of tetanus toxin light chain in Drosophila specifically eliminates synaptic transmission and causes behavioral defects. Neuron. 1995; 14: 341-51.

Hoch M, Broadie K, J├Ąckle H, Skaer H. Sequential fates in a single cell are established by the neurogenic cascade in the Malpighian tubules of Drosophila. Development. 1994 Dec; 120(12): 3439-50. PMID: 7821213, ISSN: 0950-1991.

Broadie K, Bellen HJ, DiAntonio A, Littleton JT, Schwarz TL. Absence of synaptotagmin disrupts excitation-secretion coupling during synaptic transmission. Proc. Natl. Acad. Sci. U.S.A. 1994 Oct 10/25/1994; 91(22): 10727-31. PMID: 7938019, PMCID: PMC45095, ISSN: 0027-8424.

Harrison SD, Broadie K, van de Goor J, Rubin GM. Mutations in the Drosophila Rop gene suggest a function in general secretion and synaptic transmission. Neuron. 1994 Sep; 13(3): 555-66. PMID: 7917291, PII: 0896-6273(94)90025-6, ISSN: 0896-6273.

Broadie KS. Synaptogenesis in Drosophila: coupling genetics and electrophysiology. J. Physiol. Paris. 1994; 88(2): 123-39. PMID: 7833856, PII: 0928-4257(94)90023-X, ISSN: 0928-4257.

Meadows LA, Gell D, Broadie K, Gould AP, White RA. The cell adhesion molecule, connectin, and the development of the Drosophila neuromuscular system. J. Cell. Sci. 1994 Jan; 107 ( Pt 1): 321-8. PMID: 8175919, ISSN: 0021-9533.

3. Harrison, S.D., Broadie, K., van de Goor, J. and Rubin, G.M. Mutations in the Drosophila Rop gene suggest a function in general secretion and synaptic transmission. Neuron. 1994; 13: 555-66.

Broadie K, Bate M. Muscle development is independent of innervation during Drosophila embryogenesis. Development. 1993 Oct; 119(2): 533-43. PMID: 8287801, ISSN: 0950-1991.

Broadie K, Bate M. Activity-dependent development of the neuromuscular synapse during Drosophila embryogenesis. Neuron. 1993 Oct; 11(4): 607-19. PMID: 7691105, PII: 0896-6273(93)90073-Z, ISSN: 0896-6273.

Broadie K, Bate M. Innervation directs receptor synthesis and localization in Drosophila embryo synaptogenesis. Nature. 1993 Jan 1/28/1993; 361(6410): 350-3. PMID: 8426654, DOI: 10.1038/361350a0, ISSN: 0028-0836.

Broadie KS, Bate M. Development of larval muscle properties in the embryonic myotubes of Drosophila melanogaster. J. Neurosci. 1993 Jan; 13(1): 167-80. PMID: 8093714, ISSN: 0270-6474.

Broadie KS, Bate M. Development of the embryonic neuromuscular synapse of Drosophila melanogaster. J. Neurosci. 1993 Jan; 13(1): 144-66. PMID: 8093713, ISSN: 0270-6474.

Broadie K, Sink H, Van Vactor D, Fambrough D, Whitington PM, Bate M, Goodman CS. From growth cone to synapse: the life history of the RP3 motor neuron. Dev. Suppl. 1993; 227-38. PMID: 8049478.

Broadie KS, Bate M. The development of adult muscles in Drosophila: ablation of identified muscle precursor cells. Development. 1991 Sep; 113(1): 103-18. PMID: 1764988, ISSN: 0950-1991.

Tublitz N, Brink D, Broadie KS, Loi PK, Sylwester AW. From behavior to molecules: an integrated approach to the study of neuropeptides. Trends Neurosci. 1991 Jun; 14(6): 254-9. PMID: 1716020, PII: 0166-2236(91)90126-F, ISSN: 0166-2236.

Broadie KS, Sylwester AW, Bate M, Tublitz NJ. Immunological, biochemical and physiological analyses of cardioacceleratory peptide 2 (CAP2) activity in the embryo of the tobacco hawkmoth Manduca sexta. Development. 1990 Jan; 108(1): 59-71. PMID: 2351066, ISSN: 0950-1991.

Available Postdoctoral Position Details
Posted: 7/7/2016

WE ARE ACTIVELY RECRUITING POSTDOCTORAL RESEARCHERS. We are interested in recruiting cellular and molecular biologists interested in neurobiology, particularly synaptic biology. We are particularly keen to recruit experienced electrophysiologists (patch-clamp, TEVC) and confocal microscopists (live imaging, optogenetics). We are also looking for molecular geneticists with experience in Drosophila or other genetic models (yeast, C. elegans, zebrafish).

We use genetic approaches to study the nervous system, particularly the development, function and plasticity of neuronal synapses. We model many human diseases which involve synaptic dysfunction, including Fragile X syndrome. We use a multidisciplinary approach coupling molecular genetics with neurological techniques including electrophysiology, optical imaging and EM.

If you have an interest, please contact me directly at All inquiries should be accompanied by a CV and statement of specific interests.