Mark D. Does, Ph.D.


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Faculty Appointments
Professor of Biomedical Engineering Professor of Electrical EngineeringProfessor of Radiology and Radiological Sciences
Ph.D., Biomedical Engineering, University of Alberta, Alberta, CanadaM.S., Electrical Engineering, University of Alberta, Alberta, CanadaB.S., Electrical Engineering, University of Alberta, Alberta, Canada
Office Address
1161 21st Avenue South
AA-1105 MCN
Nashville, TN 37232-2310
Research Description
Mark Does, Ph.D., received a bachelor of science and a master of science in electrical engineering, and a doctorate in biomedical engineering from the University of Alberta. He joined the Vanderbilt faculty in 2002 as part of the establishment of the Institute of Imaging Sciences. He is Professor of Biomedical Engineering, Electrical Engineering, and Radiology and Radiological Sciences, and he serves as the Director of the Center for Small Animal Imaging. In 2014, Dr. Does was elected into the American Institute for Medical and Biological Engineering’s (AIMBE) College of Fellows.

The Does lab is motivated by development, evaluation, and application of magnetic resonance imaging (MRI) methods for characterizing tissue microstructure, composition, and function. To this end, we develop novel MRI pulse sequences and analysis methods; evaluate and apply methods in studies of humans and small animal models of disease/injury/abnormal development; and develop and utilize statistical methods and computational models to predict and explain MRI contrast in tissues.

Current Research Projects:

-Characterization of Neural Tissue and Skeletal Muscle with MRI
using Relaxation, Magnetization Transfer, and Diffusion

-Compartmental Modelling of Water Proton Relaxation and Diffusion
with computational methods, chemical phantoms, and compartment-specific contrast agents

-Cortical Bone Imaging with Magnetic Resonance

-Development/Optimization of MRI pulse sequence
for rapid relaxometry
for diffusion measurement at very short diffusion times
for small animal cardiac imaging,
for MR microscopy
Research Keywords
MRI, Small Animal Imaging
West KL, Kelm ND, Carson RP, Does MD. A revised model for estimating g-ratio from MRI. Neuroimage [print-electronic]. 2016 Jan 1/15/2016; 125: 1155-8. PMID: 26299793, PMCID: PMC4691384, PII: S1053-8119(15)00726-0, DOI: 10.1016/j.neuroimage.2015.08.017, ISSN: 1095-9572.

Kelm ND, West KL, Carson RP, Gochberg DF, Ess KC, Does MD. Evaluation of diffusion kurtosis imaging in ex vivo hypomyelinated mouse brains. Neuroimage [print-electronic]. 2016 Jan 1/1/2016; 124(Pt A): 612-26. PMID: 26400013, PMCID: PMC4651761, PII: S1053-8119(15)00841-1, DOI: 10.1016/j.neuroimage.2015.09.028, ISSN: 1095-9572.

Manhard MK, Horch RA, Gochberg DF, Nyman JS, Does MD. In Vivo Quantitative MR Imaging of Bound and Pore Water in Cortical Bone. Radiology [print-electronic]. 2015 Oct; 277(1): 221-9. PMID: 26020434, PMCID: PMC4613881, DOI: 10.1148/radiol.2015140336, ISSN: 1527-1315.

Lankford CL, Dortch RD, Does MD. Fast T2 mapping with multiple echo, Caesar cipher acquisition and model-based reconstruction. Magn Reson Med [print-electronic]. 2015 Mar; 73(3): 1065-74. PMID: 24753216, PMCID: PMC4201905, DOI: 10.1002/mrm.25221, ISSN: 1522-2594.

Horch RA, Gochberg DF, Nyman JS, Does MD. Clinically compatible MRI strategies for discriminating bound and pore water in cortical bone. Magn Reson Med [print-electronic]. 2012 Dec; 68(6): 1774-84. PMID: 22294340, PMCID: PMC3357454, DOI: 10.1002/mrm.24186, ISSN: 1522-2594.

Zu Z, Janve VA, Li K, Does MD, Gore JC, Gochberg DF. Multi-angle ratiometric approach to measure chemical exchange in amide proton transfer imaging. Magn Reson Med [print-electronic]. 2012 Sep; 68(3): 711-9. PMID: 22161770, PMCID: PMC3389148, DOI: 10.1002/mrm.23276, ISSN: 1522-2594.

Harkins KD, Dula AN, Does MD. Effect of intercompartmental water exchange on the apparent myelin water fraction in multiexponential T2 measurements of rat spinal cord. Magn Reson Med [print-electronic]. 2012 Mar; 67(3): 793-800. PMID: 21713984, PMCID: PMC3188335, DOI: 10.1002/mrm.23053, ISSN: 1522-2594.

Zu Z, Li K, Janve VA, Does MD, Gochberg DF. Optimizing pulsed-chemical exchange saturation transfer imaging sequences. Magn Reson Med [print-electronic]. 2011 Oct; 66(4): 1100-8. PMID: 21432903, PMCID: PMC3151337, DOI: 10.1002/mrm.22884, ISSN: 1522-2594.

Horch RA, Gore JC, Does MD. Origins of the ultrashort-T2 1H NMR signals in myelinated nerve: a direct measure of myelin content?. Magn Reson Med [print-electronic]. 2011 Jul; 66(1): 24-31. PMID: 21574183, PMCID: PMC3120910, DOI: 10.1002/mrm.22980, ISSN: 1522-2594.

Xu J, Xie J, Jourquin J, Colvin DC, Does MD, Quaranta V, Gore JC. Influence of cell cycle phase on apparent diffusion coefficient in synchronized cells detected using temporal diffusion spectroscopy. Magn Reson Med [print-electronic]. 2011 Apr; 65(4): 920-6. PMID: 21413058, PMCID: PMC3433804, DOI: 10.1002/mrm.22704, ISSN: 1522-2594.

Colvin DC, Loveless ME, Does MD, Yue Z, Yankeelov TE, Gore JC. Earlier detection of tumor treatment response using magnetic resonance diffusion imaging with oscillating gradients. Magn Reson Imaging [print-electronic]. 2011 Apr; 29(3): 315-23. PMID: 21190804, PMCID: PMC3285502, PII: S0730-725X(10)00356-5, DOI: 10.1016/j.mri.2010.10.003, ISSN: 1873-5894.

Xu J, Does MD, Gore JC. Dependence of temporal diffusion spectra on microstructural properties of biological tissues. Magn Reson Imaging [print-electronic]. 2011 Apr; 29(3): 380-90. PMID: 21129880, PMCID: PMC3058152, PII: S0730-725X(10)00320-6, DOI: 10.1016/j.mri.2010.10.002, ISSN: 1873-5894.

Colvin DC, Jourquin J, Xu J, Does MD, Estrada L, Gore JC. Effects of intracellular organelles on the apparent diffusion coefficient of water molecules in cultured human embryonic kidney cells. Magn Reson Med [print-electronic]. 2011 Mar; 65(3): 796-801. PMID: 21337411, PMCID: PMC3285501, DOI: 10.1002/mrm.22666, ISSN: 1522-2594.

Horch RA, Gochberg DF, Nyman JS, Does MD. Non-invasive predictors of human cortical bone mechanical properties: T(2)-discriminated H NMR compared with high resolution X-ray. PLoS ONE. 2011; 6(1): e16359. PMID: 21283693, PMCID: PMC3025007, DOI: 10.1371/journal.pone.0016359, ISSN: 1932-6203.

Horch RA, Wilkens K, Gochberg DF, Does MD. RF coil considerations for short-T2 MRI. Magn Reson Med. 2010 Dec; 64(6): 1652-7. PMID: 20665825, PMCID: PMC2970722, DOI: 10.1002/mrm.22558, ISSN: 1522-2594.

Dortch RD, Apker GA, Valentine WM, Lai B, Does MD. Compartment-specific enhancement of white matter and nerve ex vivo using chromium. Magn Reson Med. 2010 Sep; 64(3): 688-97. PMID: 20806376, PMCID: PMC2933086, DOI: 10.1002/mrm.22460, ISSN: 1522-2594.

Horch RA, Nyman JS, Gochberg DF, Dortch RD, Does MD. Characterization of 1H NMR signal in human cortical bone for magnetic resonance imaging. Magn Reson Med. 2010 Sep; 64(3): 680-7. PMID: 20806375, PMCID: PMC2933073, DOI: 10.1002/mrm.22459, ISSN: 1522-2594.

Gore JC, Xu J, Colvin DC, Yankeelov TE, Parsons EC, Does MD. Characterization of tissue structure at varying length scales using temporal diffusion spectroscopy. NMR Biomed. 2010 Aug; 23(7): 745-56. PMID: 20677208, PMCID: PMC3285511, DOI: 10.1002/nbm.1531, ISSN: 1099-1492.

Li K, Zu Z, Xu J, Janve VA, Gore JC, Does MD, Gochberg DF. Optimized inversion recovery sequences for quantitative T1 and magnetization transfer imaging. Magn Reson Med. 2010 Aug; 64(2): 491-500. PMID: 20665793, PMCID: PMC2943213, DOI: 10.1002/mrm.22440, ISSN: 1522-2594.

Kobukai S, Baheza R, Cobb JG, Virostko J, Xie J, Gillman A, Koktysh D, Kerns D, Does M, Gore JC, Pham W. Magnetic nanoparticles for imaging dendritic cells. Magn Reson Med. 2010 May; 63(5): 1383-90. PMID: 20432309, DOI: 10.1002/mrm.22313, ISSN: 1522-2594.

Dula AN, Gochberg DF, Valentine HL, Valentine WM, Does MD. Multiexponential T2, magnetization transfer, and quantitative histology in white matter tracts of rat spinal cord. Magn Reson Med. 2010 Apr; 63(4): 902-9. PMID: 20373391, PMCID: PMC2852261, DOI: 10.1002/mrm.22267, ISSN: 1522-2594.

Dortch RD, Horch RA, Does MD. Development, simulation, and validation of NMR relaxation-based exchange measurements. J Chem Phys. 2009 Oct 10/28/2009; 131(16): 164502. PMID: 19894951, PMCID: PMC2776779, DOI: 10.1063/1.3245866, ISSN: 1089-7690.

Xu J, Does MD, Gore JC. Quantitative characterization of tissue microstructure with temporal diffusion spectroscopy. J. Magn. Reson [print-electronic]. 2009 Oct; 200(2): 189-97. PMID: 19616979, PMCID: PMC2758625, PII: S1090-7807(09)00189-X, DOI: 10.1016/j.jmr.2009.06.022, ISSN: 1096-0856.

Viquez OM, Lai B, Ahn JH, Does MD, Valentine HL, Valentine WM. N,N-diethyldithiocarbamate promotes oxidative stress prior to myelin structural changes and increases myelin copper content. Toxicol. Appl. Pharmacol [print-electronic]. 2009 Aug 8/15/2009; 239(1): 71-9. PMID: 19467251, PMCID: PMC2727155, PII: S0041-008X(09)00217-8, DOI: 10.1016/j.taap.2009.05.017, ISSN: 1096-0333.

Dortch RD, Yankeelov TE, Yue Z, Quarles CC, Gore JC, Does MD. Evidence of multiexponential T2 in rat glioblastoma. NMR Biomed. 2009 Jul; 22(6): 609-18. PMID: 19267385, PMCID: PMC4178926, DOI: 10.1002/nbm.1374, ISSN: 1099-1492.

Xu J, Does MD, Gore JC. Sensitivity of MR diffusion measurements to variations in intracellular structure: effects of nuclear size. Magn Reson Med. 2009 Apr; 61(4): 828-33. PMID: 19205020, PMCID: PMC2749035, DOI: 10.1002/mrm.21793, ISSN: 1522-2594.

Louie EA, Gochberg DF, Does MD, Damon BM. Transverse relaxation and magnetization transfer in skeletal muscle: effect of pH. Magn Reson Med. 2009 Mar; 61(3): 560-9. PMID: 19097244, PMCID: PMC2649690, DOI: 10.1002/mrm.21847, ISSN: 1522-2594.

Dula AN, Gochberg DF, Does MD. Optimal echo spacing for multi-echo imaging measurements of Bi-exponential T2 relaxation. J. Magn. Reson [print-electronic]. 2009 Feb; 196(2): 149-56. PMID: 19028432, PMCID: PMC2694452, PII: S1090-7807(08)00358-3, DOI: 10.1016/j.jmr.2008.11.002, ISSN: 1096-0856.

Colvin DC, Yankeelov TE, Does MD, Yue Z, Quarles C, Gore JC. New insights into tumor microstructure using temporal diffusion spectroscopy. Cancer Res. 2008 Jul 7/15/2008; 68(14): 5941-7. PMID: 18632649, PMCID: PMC2719758, PII: 68/14/5941, DOI: 10.1158/0008-5472.CAN-08-0832, ISSN: 1538-7445.

Fan RH, Does MD. Compartmental relaxation and diffusion tensor imaging measurements in vivo in lambda-carrageenan-induced edema in rat skeletal muscle. NMR Biomed. 2008 Jul; 21(6): 566-73. PMID: 18041804, PMCID: PMC2694448, DOI: 10.1002/nbm.1226, ISSN: 0952-3480.

Qin Q, Gore JC, de Graaf RA, Does MD. Quantitative T2 measurement of a single voxel with arbitrary shape using pinwheel excitation and CPMG acquisition. MAGMA [print-electronic]. 2007 Dec; 20(5-6): 233-40. PMID: 17999101, PMCID: PMC2634838, DOI: 10.1007/s10334-007-0088-9, ISSN: 0968-5243.

Dortch RD, Does MD. Temporal DeltaB0 and relaxation in the rat heart. Magn Reson Med. 2007 Nov; 58(5): 939-46. PMID: 17969107, PMCID: PMC2696614, DOI: 10.1002/mrm.21299, ISSN: 0740-3194.

Eagleson KL, Schlueter McFadyen-Ketchum LJ, Ahrens ET, Mills PH, Does MD, Nickols J, Levitt P. Disruption of Foxg1 expression by knock-in of cre recombinase: effects on the development of the mouse telencephalon. Neuroscience [print-electronic]. 2007 Aug 8/24/2007; 148(2): 385-99. PMID: 17640820, PMCID: PMC2194757, PII: S0306-4522(07)00740-3, DOI: 10.1016/j.neuroscience.2007.06.012, ISSN: 0306-4522.

Byrd JB, Shreevatsa A, Putlur P, Foretia D, McAlexander L, Sinha T, Does MD, Brown NJ. Dipeptidyl peptidase IV deficiency increases susceptibility to angiotensin-converting enzyme inhibitor-induced peritracheal edema. J. Allergy Clin. Immunol [print-electronic]. 2007 Aug; 120(2): 403-8. PMID: 17531305, PII: S0091-6749(07)00760-9, DOI: 10.1016/j.jaci.2007.04.012, ISSN: 0091-6749.

Qin Q, Gore JC, Does MD, Avison MJ, de Graaf RA. 2D arbitrary shape-selective excitation summed spectroscopy (ASSESS). Magn Reson Med. 2007 Jul; 58(1): 19-26. PMID: 17659614, DOI: 10.1002/mrm.21274, ISSN: 0740-3194.

Valentine HL, Does MD, Marshall V, Tonkin EG, Valentine WM. Multicomponent T2 analysis of dithiocarbamate-mediated peripheral nerve demyelination. Neurotoxicology [print-electronic]. 2007 May; 28(3): 645-54. PMID: 17350098, PMCID: PMC1976306, PII: S0161-813X(07)00029-0, DOI: 10.1016/j.neuro.2007.01.009, ISSN: 0161-813X.

Xu J, Does MD, Gore JC. Numerical study of water diffusion in biological tissues using an improved finite difference method. Phys Med Biol [print-electronic]. 2007 Apr 4/7/2007; 52(7): N111-26. PMID: 17374905, PMCID: PMC2634837, PII: S0031-9155(07)33948-1, DOI: 10.1088/0031-9155/52/7/N01, ISSN: 0031-9155.

Horch RA, Does MD. Aqueous urea as a model system for bi-exponential relaxation. MAGMA [print-electronic]. 2007 Feb; 20(1): 51-6. PMID: 17260153, DOI: 10.1007/s10334-006-0064-9, ISSN: 0968-5243.

Andrews TJ, Osborne MT, Does MD. Diffusion of myelin water. Magn Reson Med. 2006 Aug; 56(2): 381-5. PMID: 16767712, DOI: 10.1002/mrm.20945, ISSN: 0740-3194.

Parsons EC, Does MD, Gore JC. Temporal diffusion spectroscopy: theory and implementation in restricted systems using oscillating gradients. Magn Reson Med. 2006 Jan; 55(1): 75-84. PMID: 16342147, DOI: 10.1002/mrm.20732, ISSN: 0740-3194.

Travis AR, Does MD. Selective excitation of myelin water using inversion-recovery-based preparations. Magn Reson Med. 2005 Sep; 54(3): 743-7. PMID: 16088884, DOI: 10.1002/mrm.20606, ISSN: 0740-3194.

Does MD. Relaxation-selective magnetization preparation based on T1 and T2. J. Magn. Reson. 2005 Feb; 172(2): 306-11. PMID: 15649757, PII: S1090-7807(04)00370-2, DOI: 10.1016/j.jmr.2004.11.008, ISSN: 1090-7807.

Rybicki AC, Fabry ME, Does MD, Kaul DK, Nagel RL. Differential gene expression in the kidney of sickle cell transgenic mice: upregulated genes. Blood Cells Mol. Dis. 2003 Nov; 31(3): 370-80. PMID: 14636654, PII: S1079979603002122, ISSN: 1079-9796.

Parsons EC, Does MD, Gore JC. Modified oscillating gradient pulses for direct sampling of the diffusion spectrum suitable for imaging sequences. Magn Reson Imaging. 2003 Apr; 21(3-4): 279-85. PMID: 12850719, PII: S0730725X03001553, ISSN: 0730-725X.

Does MD, Parsons EC, Gore JC. Oscillating gradient measurements of water diffusion in normal and globally ischemic rat brain. Magn Reson Med. 2003 Feb; 49(2): 206-15. PMID: 12541239, DOI: 10.1002/mrm.10385, ISSN: 0740-3194.

Dutka MV, Scanley BE, Does MD, Gore JC. Changes in CBF-BOLD coupling detected by MRI during and after repeated transient hypercapnia in rat. Magn Reson Med. 2002 Aug; 48(2): 262-70. PMID: 12210934, DOI: 10.1002/mrm.10217, ISSN: 0740-3194.

Does MD, Gore JC. Compartmental study of T(1) and T(2) in rat brain and trigeminal nerve in vivo. Magn Reson Med. 2002 Feb; 47(2): 274-83. PMID: 11810670, PII: 10.1002/mrm.10060, ISSN: 0740-3194.

Fong PM, Keil DC, Does MD, Gore JC. Polymer gels for magnetic resonance imaging of radiation dose distributions at normal room atmosphere. Phys Med Biol. 2001 Dec; 46(12): 3105-13. PMID: 11768494, ISSN: 0031-9155.

Gore JC, Anderson AW, Does MD, Gochberg DF, Joers JM, Kennan RP, Parsons EC, Schachter M. The relationship of problems in biomedical MRI to the study of porous media. Magn Reson Imaging. 2001 Apr; 19(3-4): 295-300. PMID: 11445302, PII: S0730-725X(01)00239-9, ISSN: 0730-725X.

Schachter M, Does MD, Anderson AW, Gore JC. Measurements of restricted diffusion using an oscillating gradient spin-echo sequence. J. Magn. Reson. 2000 Dec; 147(2): 232-7. PMID: 11097814, PII: S1090-7807(00)92203-1, DOI: 10.1006/jmre.2000.2203, ISSN: 1090-7807.

Does MD, Gore JC. Rapid acquisition transverse relaxometric imaging. J. Magn. Reson. 2000 Nov; 147(1): 116-20. PMID: 11042054, PII: S1090-7807(00)92168-2, DOI: 10.1006/jmre.2000.2168, ISSN: 1090-7807.

Does MD, Gore JC. Compartmental study of diffusion and relaxation measured in vivo in normal and ischemic rat brain and trigeminal nerve. Magn Reson Med. 2000 Jun; 43(6): 837-44. PMID: 10861878, PII: 10.1002/1522-2594(200006)43:6<837::AID-MRM9>3.0.CO;2-O, ISSN: 0740-3194.

Does MD, Gore JC. Complications of nonlinear echo time spacing for measurement of T (2). NMR Biomed. 2000 Jan; 13(1): 1-7. PMID: 10668048, PII: 10.1002/(SICI)1099-1492(200002)13:1<1::AID-NBM603>3.0.CO;2-E, ISSN: 0952-3480.

Does MD, Zhong J, Gore JC. In vivo measurement of ADC change due to intravascular susceptibility variation. Magn Reson Med. 1999 Feb; 41(2): 236-40. PMID: 10080268, PII: 10.1002/(SICI)1522-2594(199902)41:2<236::AID-MRM4>3.0.CO;2-3, ISSN: 0740-3194.

Does MD, Beaulieu C, Allen PS, Snyder RE. Multi-component T1 relaxation and magnetisation transfer in peripheral nerve. Magn Reson Imaging. 1998 Nov; 16(9): 1033-41. PMID: 9839987, PII: S0730-725X(98)00139-8, ISSN: 0730-725X.

Does MD, Snyder RE. Multiecho imaging with suboptimal spoiler gradients. J. Magn. Reson. 1998 Mar; 131(1): 25-31. PMID: 9533902, PII: S1090-7807(97)91320-3, DOI: 10.1006/jmre.1997.1320, ISSN: 1090-7807.

Beaulieu C, Does MD, Snyder RE, Allen PS. Changes in water diffusion due to Wallerian degeneration in peripheral nerve. Magn Reson Med. 1996 Oct; 36(4): 627-31. PMID: 8892217, ISSN: 0740-3194.

Does MD, Snyder RE. Multiexponential T2 relaxation in degenerating peripheral nerve. Magn Reson Med. 1996 Feb; 35(2): 207-13. PMID: 8622585, ISSN: 0740-3194.

Does MD, Snyder RE. T2 relaxation of peripheral nerve measured in vivo. Magn Reson Imaging. 1995; 13(4): 575-80. PMID: 7674853, PII: 0730-725X(94)00138-S, ISSN: 0730-725X.