Faculty Appointments
Professor of Biological Sciences
Professor of Cell and Developmental BiologyProfessor of Cell and Developmental BiologyStevenson Chair in Biological SciencesStevenson Chair in Biological Sciences
Education
Ph.D., Cell & Molecular Biology, Saint Louis University, Saint Louis, MissouriB.S., Chemistry, Maryville College, Maryville, Tennessee
Office Address
5260 MRBIII, Department of Biological Sciences
Nashville, TN 37232
Nashville, TN 37232
Research Description
The Graham laboratory studies molecular mechanisms underpinning membrane biogenesis with implications for diet-induced obesity, type 2 diabetes and neurodegenerative diseases. We work to understand how cells generate the unique composition of protein and lipid molecules in the plasma membrane and internal organelles. This entails studies on vesicle-mediated protein trafficking and lipid transporters (flippases) that establish an asymmetric distribution of lipid molecules between the two leaflets of membranes. There are currently three main projects in the laboratory.
1. The Graham laboratory discovered that a large family of type IV P-type ATPases (P4-ATPases) are phospholipid flippases that transport specific lipid molecules across membranes to establish membrane asymmetry. We continue to study structure/function relationships for these pumps to define how they recognize their unique lipid substrates and how they are regulated. The substrate preference of these enzymes can be mutationally tuned to test the consequence of lipid transport to the cell.
2. Human P4-ATPases have been implicated in several human pathologies, including type 2 diabetes, neurodegenerative diseases, liver disease and immune deficiency. We are developing new mouse models to define the role of P4-ATPase deficiency in diet-induced obesity and type 2 diabetes. The obesity epidemic is a creating a major health crisis from increased risk of heart disease, diabetes and liver disease. Our studies are providing novel insights into how transport of specific lipid molecules influences energy metabolism and insulin sensitivity.
3) The Graham lab also discovered that P4-ATPases play a crucial role in budding protein transport vesicles from Golgi and endosomal membranes. For example, a budding yeast P4-ATPase called Drs2 translocates phosphatidylserine across the membrane and this flippase activity is required to bud different types of transport vesicles from Golgi and endosomal compartments. More recently, we found that trafficking of SNARE proteins in the endosomal system requires ubiquitin-dependent packaging into COPI vesicles. Current studies are focused on characterizing novel roles of ubiquitylation on protein trafficking in the Golgi/endosomal membrane system.
1. The Graham laboratory discovered that a large family of type IV P-type ATPases (P4-ATPases) are phospholipid flippases that transport specific lipid molecules across membranes to establish membrane asymmetry. We continue to study structure/function relationships for these pumps to define how they recognize their unique lipid substrates and how they are regulated. The substrate preference of these enzymes can be mutationally tuned to test the consequence of lipid transport to the cell.
2. Human P4-ATPases have been implicated in several human pathologies, including type 2 diabetes, neurodegenerative diseases, liver disease and immune deficiency. We are developing new mouse models to define the role of P4-ATPase deficiency in diet-induced obesity and type 2 diabetes. The obesity epidemic is a creating a major health crisis from increased risk of heart disease, diabetes and liver disease. Our studies are providing novel insights into how transport of specific lipid molecules influences energy metabolism and insulin sensitivity.
3) The Graham lab also discovered that P4-ATPases play a crucial role in budding protein transport vesicles from Golgi and endosomal membranes. For example, a budding yeast P4-ATPase called Drs2 translocates phosphatidylserine across the membrane and this flippase activity is required to bud different types of transport vesicles from Golgi and endosomal compartments. More recently, we found that trafficking of SNARE proteins in the endosomal system requires ubiquitin-dependent packaging into COPI vesicles. Current studies are focused on characterizing novel roles of ubiquitylation on protein trafficking in the Golgi/endosomal membrane system.
Research Keywords
Protein transport and sorting; Coated vesicles; Membrane biogenesis; COPI; Clathrin; P-type ATPases; Transporters; Ubiquitin; Ubiquitylation; Neurodegenerative disease; Obesity, Type 2 diabetes; Lipids; Lipid Transport; Energy metabolism; Insulin resistance; Genetics, Molecular biology, Protein structure and function, Biochemistry, Cell Biology
Publications
Takar M, Huang Y, Graham TR. The PQ-loop protein Any1 segregates Drs2 and Neo1 functions required for viability and plasma membrane phospholipid asymmetry. J. Lipid Res [print-electronic]. 2019 May; 60(5): 1032-42. PMID: 30824614, PMCID: PMC6495175, PII: jlr.M093526, DOI: 10.1194/jlr.M093526, ISSN: 1539-7262.
Best JT, Xu P, Graham TR. Phospholipid flippases in membrane remodeling and transport carrier biogenesis. Curr. Opin. Cell Biol [print-electronic]. 2019 Mar 3/18/2019; 59: 8-15. PMID: 30897446, PII: S0955-0674(19)30001-8, DOI: 10.1016/j.ceb.2019.02.004, ISSN: 1879-0410.
Roland BP, Naito T, Best JT, Arnaiz-Yépez C, Takatsu H, Yu RJ, Shin HW, Graham TR. Yeast and human P4-ATPases transport glycosphingolipids using conserved structural motifs. J. Biol. Chem [print-electronic]. 2019 Feb 2/8/2019; 294(6): 1794-806. PMID: 30530492, PMCID: PMC6369285, PII: RA118.005876, DOI: 10.1074/jbc.RA118.005876, ISSN: 1083-351X.
Xu P, Hankins HM, MacDonald C, Erlinger SJ, Frazier MN, Diab NS, Piper RC, Jackson LP, MacGurn JA, Graham TR. COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal. Elife. 2017 Oct 10/23/2017; 6: PMID: 29058666, PMCID: PMC5663479, PII: 28342, DOI: 10.7554/eLife.28342, ISSN: 2050-084X.
van Leeuwen J, Pons C, Mellor JC, Yamaguchi TN, Friesen H, Koschwanez J, Ušaj MM, Pechlaner M, Takar M, Ušaj M, VanderSluis B, Andrusiak K, Bansal P, Baryshnikova A, Boone CE, Cao J, Cote A, Gebbia M, Horecka G, Horecka I, Kuzmin E, Legro N, Liang W, van Lieshout N, McNee M, San Luis BJ, Shaeri F, Shuteriqi E, Sun S, Yang L, Youn JY, Yuen M, Costanzo M, Gingras AC, Aloy P, Oostenbrink C, Murray A, Graham TR, Myers CL, Andrews BJ, Roth FP, Boone C. Exploring genetic suppression interactions on a global scale. Science. 2016 Nov 11/4/2016; 354(6312): PMID: 27811238, PII: 354/6312/aag0839, DOI: 10.1126/science.aag0839, ISSN: 1095-9203.
Roland BP, Graham TR. Directed evolution of a sphingomyelin flippase reveals mechanism of substrate backbone discrimination by a P4-ATPase. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2016 Aug 8/2/2016; 113(31): E4460-6. PMID: 27432949, PMCID: PMC4978280, PII: 1525730113, DOI: 10.1073/pnas.1525730113, ISSN: 1091-6490.
Takar M, Wu Y, Graham TR. The Essential Neo1 Protein from Budding Yeast Plays a Role in Establishing Aminophospholipid Asymmetry of the Plasma Membrane. J. Biol. Chem [print-electronic]. 2016 Jul 7/22/2016; 291(30): 15727-39. PMID: 27235400, PMCID: PMC4957055, PII: M115.686253, DOI: 10.1074/jbc.M115.686253, ISSN: 1083-351X.
Wu Y, Takar M, Cuentas-Condori AA, Graham TR. Neo1 and phosphatidylethanolamine contribute to vacuole membrane fusion in Saccharomyces cerevisiae. Cell Logist. 2016 Jul; 6(3): e1228791. PMID: 27738552, PMCID: PMC5058351, PII: 1228791, DOI: 10.1080/21592799.2016.1228791, ISSN: 2159-2780.
Roland BP, Graham TR. Decoding P4-ATPase substrate interactions. Crit. Rev. Biochem. Mol. Biol [print-electronic]. 2015 Dec; 51(6): 513-27. PMID: 27696908, PMCID: PMC5285478, DOI: 10.1080/10409238.2016.1237934, ISSN: 1549-7798.
Hankins HM, Sere YY, Diab NS, Menon AK, Graham TR. Phosphatidylserine translocation at the yeast trans-Golgi network regulates protein sorting into exocytic vesicles. Mol. Biol. Cell [print-electronic]. 2015 Dec 12/15/2015; 26(25): 4674-85. PMID: 26466678, PMCID: PMC4678023, PII: mbc.E15-07-0487, DOI: 10.1091/mbc.E15-07-0487, ISSN: 1939-4586.
Hankins HM, Baldridge RD, Xu P, Graham TR. Role of flippases, scramblases and transfer proteins in phosphatidylserine subcellular distribution. Traffic [print-electronic]. 2015 Jan; 16(1): 35-47. PMID: 25284293, PMCID: PMC4275391, DOI: 10.1111/tra.12233, ISSN: 1600-0854.
Zhou X, Sebastian TT, Graham TR. Auto-inhibition of Drs2p, a yeast phospholipid flippase, by its carboxyl-terminal tail. J. Biol. Chem [print-electronic]. 2013 Nov 11/1/2013; 288(44): 31807-15. PMID: 24045945, PMCID: PMC3814774, PII: M113.481986, DOI: 10.1074/jbc.M113.481986, ISSN: 1083-351X.
Xu P, Baldridge RD, Chi RJ, Burd CG, Graham TR. Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport. J. Cell Biol [print-electronic]. 2013 Sep 9/16/2013; 202(6): 875-86. PMID: 24019533, PMCID: PMC3776346, PII: jcb.201305094, DOI: 10.1083/jcb.201305094, ISSN: 1540-8140.
Baldridge RD, Xu P, Graham TR. Type IV P-type ATPases distinguish mono- versus diacyl phosphatidylserine using a cytofacial exit gate in the membrane domain. J. Biol. Chem [print-electronic]. 2013 Jul 7/5/2013; 288(27): 19516-27. PMID: 23709217, PMCID: PMC3707653, PII: M113.476911, DOI: 10.1074/jbc.M113.476911, ISSN: 1083-351X.
Graham TR. Arl1 gets into the membrane remodeling business with a flippase and ArfGEF. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Feb 2/19/2013; 110(8): 2691-2. PMID: 23401560, PMCID: PMC3581979, PII: 1300420110, DOI: 10.1073/pnas.1300420110, ISSN: 1091-6490.
Baldridge RD, Graham TR. Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Jan 1/29/2013; 110(5): E358-67. PMID: 23302692, PMCID: PMC3562821, PII: 1216948110, DOI: 10.1073/pnas.1216948110, ISSN: 1091-6490.
Sebastian TT, Baldridge RD, Xu P, Graham TR. Phospholipid flippases: building asymmetric membranes and transport vesicles. Biochim. Biophys. Acta [print-electronic]. 2012 Aug; 1821(8): 1068-77. PMID: 22234261, PMCID: PMC3368091, PII: S1388-1981(11)00272-1, DOI: 10.1016/j.bbalip.2011.12.007, ISSN: 0006-3002.
Baldridge RD, Graham TR. Identification of residues defining phospholipid flippase substrate specificity of type IV P-type ATPases. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Feb 2/7/2012; 109(6): E290-8. PMID: 22308393, PMCID: PMC3277569, PII: 1115725109, DOI: 10.1073/pnas.1115725109, ISSN: 1091-6490.
Graham TR, Burd CG. Coordination of Golgi functions by phosphatidylinositol 4-kinases. Trends Cell Biol [print-electronic]. 2011 Feb; 21(2): 113-21. PMID: 21282087, PMCID: PMC3053015, PII: S0962-8924(10)00214-X, DOI: 10.1016/j.tcb.2010.10.002, ISSN: 1879-3088.
Graham TR, Kozlov MM. Interplay of proteins and lipids in generating membrane curvature. Curr. Opin. Cell Biol [print-electronic]. 2010 Aug; 22(4): 430-6. PMID: 20605711, PMCID: PMC3770468, PII: S0955-0674(10)00065-7, DOI: 10.1016/j.ceb.2010.05.002, ISSN: 1879-0410.
Natarajan P, Liu K, Patil DV, Sciorra VA, Jackson CL, Graham TR. Regulation of a Golgi flippase by phosphoinositides and an ArfGEF. Nat. Cell Biol [print-electronic]. 2009 Dec; 11(12): 1421-6. PMID: 19898464, PMCID: PMC2787759, PII: ncb1989, DOI: 10.1038/ncb1989, ISSN: 1476-4679.
Zhou X, Graham TR. Reconstitution of phospholipid translocase activity with purified Drs2p, a type-IV P-type ATPase from budding yeast. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2009 Sep 9/29/2009; 106(39): 16586-91. PMID: 19805341, PMCID: PMC2757829, PII: 0904293106, DOI: 10.1073/pnas.0904293106, ISSN: 1091-6490.
Muthusamy BP, Natarajan P, Zhou X, Graham TR. Linking phospholipid flippases to vesicle-mediated protein transport. Biochim. Biophys. Acta [print-electronic]. 2009 Jul; 1791(7): 612-9. PMID: 19286470, PMCID: PMC3770137, PII: S1388-1981(09)00072-9, DOI: 10.1016/j.bbalip.2009.03.004, ISSN: 0006-3002.
Muthusamy BP, Raychaudhuri S, Natarajan P, Abe F, Liu K, Prinz WA, Graham TR. Control of protein and sterol trafficking by antagonistic activities of a type IV P-type ATPase and oxysterol binding protein homologue. Mol. Biol. Cell [print-electronic]. 2009 Jun; 20(12): 2920-31. PMID: 19403696, PMCID: PMC2695799, PII: E08-10-1036, DOI: 10.1091/mbc.E08-10-1036, ISSN: 1939-4586.
Ho CH, Magtanong L, Barker SL, Gresham D, Nishimura S, Natarajan P, Koh JLY, Porter J, Gray CA, Andersen RJ, Giaever G, Nislow C, Andrews B, Botstein D, Graham TR, Yoshida M, Boone C. A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds. Nat. Biotechnol [print-electronic]. 2009 Apr; 27(4): 369-77. PMID: 19349972, PMCID: PMC3856559, DOI: 10.1038/nbt.1534, ISSN: 1546-1696.
Ho CH, Magtanong L, Barker SL, Gresham D, Nishimura S, Natarajan P, Koh JLY, Porter J, Gray CA, Andersen RJ, Giaever G, Nislow C, Andrews B, Botstein D, Graham TR, Yoshida M, Boone C. A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds. Nat. Biotechnol [print-electronic]. 2009 Apr; 27(4): 369-77. PMID: 19349972, PMCID: PMC3856559, DOI: 10.1038/nbt.1534, ISSN: 1546-1696.
Liu K, Surendhran K, Nothwehr SF, Graham TR. P4-ATPase requirement for AP-1/clathrin function in protein transport from the trans-Golgi network and early endosomes. Mol. Biol. Cell [print-electronic]. 2008 Aug; 19(8): 3526-35. PMID: 18508916, PMCID: PMC2488278, PII: E08-01-0025, DOI: 10.1091/mbc.E08-01-0025, ISSN: 1939-4586.
Liu K, Hua Z, Nepute JA, Graham TR. Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Mol. Biol. Cell [print-electronic]. 2007 Feb; 18(2): 487-500. PMID: 17122361, PMCID: PMC1783782, PII: E06-07-0592, DOI: 10.1091/mbc.E06-07-0592, ISSN: 1059-1524.
Chen S, Wang J, Muthusamy BP, Liu K, Zare S, Andersen RJ, Graham TR. Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane. Traffic [print-electronic]. 2006 Nov; 7(11): 1503-17. PMID: 16956384, PII: TRA485, DOI: 10.1111/j.1600-0854.2006.00485.x, ISSN: 1398-9219.
Parsons AB, Lopez A, Givoni IE, Williams DE, Gray CA, Porter J, Chua G, Sopko R, Brost RL, Ho CH, Wang J, Ketela T, Brenner C, Brill JA, Fernandez GE, Lorenz TC, Payne GS, Ishihara S, Ohya Y, Andrews B, Hughes TR, Frey BJ, Graham TR, Andersen RJ, Boone C. Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast. Cell. 2006 Aug 8/11/2006; 126(3): 611-25. PMID: 16901791, PII: S0092-8674(06)00906-8, DOI: 10.1016/j.cell.2006.06.040, ISSN: 0092-8674.
Xiao J, Kim LS, Graham TR. Dissection of Swa2p/auxilin domain requirements for cochaperoning Hsp70 clathrin-uncoating activity in vivo. Mol. Biol. Cell [print-electronic]. 2006 Jul; 17(7): 3281-90. PMID: 16687570, PMCID: PMC1483056, PII: E06-02-0106, DOI: 10.1091/mbc.E06-02-0106, ISSN: 1059-1524.
Natarajan P, Graham TR. Measuring translocation of fluorescent lipid derivatives across yeast Golgi membranes. Methods. 2006 Jun; 39(2): 163-8. PMID: 16828307, PII: S1046-2023(06)00080-6, DOI: 10.1016/j.ymeth.2006.05.009, ISSN: 1046-2023.
Graham TR. Flippases and vesicle-mediated protein transport. Trends Cell Biol. 2004 Dec; 14(12): 670-7. PMID: 15564043, PII: S0962-8924(04)00287-9, DOI: 10.1016/j.tcb.2004.10.008, ISSN: 0962-8924.
Natarajan P, Wang J, Hua Z, Graham TR. Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2004 Jul 7/20/2004; 101(29): 10614-9. PMID: 15249668, PMCID: PMC489982, PII: 0404146101, DOI: 10.1073/pnas.0404146101, ISSN: 0027-8424.
Graham TR. Membrane targeting: getting Arl to the Golgi. Curr. Biol. 2004 Jun 6/22/2004; 14(12): R483-5. PMID: 15203023, PII: S096098220400421X, DOI: 10.1016/j.cub.2004.06.017, ISSN: 0960-9822.
Chim N, Gall WE, Xiao J, Harris MP, Graham TR, Krezel AM. Solution structure of the ubiquitin-binding domain in Swa2p from Saccharomyces cerevisiae. Proteins. 2004 Mar 3/1/2004; 54(4): 784-93. PMID: 14997574, DOI: 10.1002/prot.10636, ISSN: 1097-0134.
Chantalat S, Park SK, Hua Z, Liu K, Gobin R, Peyroche A, Rambourg A, Graham TR, Jackson CL. The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae. J. Cell. Sci [print-electronic]. 2004 Feb 2/15/2004; 117(Pt 5): 711-22. PMID: 14734650, PII: jcs.00896, DOI: 10.1242/jcs.00896, ISSN: 0021-9533.
Hua Z, Graham TR. Requirement for neo1p in retrograde transport from the Golgi complex to the endoplasmic reticulum. Mol. Biol. Cell [print-electronic]. 2003 Dec; 14(12): 4971-83. PMID: 12960419, PMCID: PMC284799, PII: E03-07-0463, DOI: 10.1091/mbc.E03-07-0463, ISSN: 1059-1524.
Gall WE, Geething NC, Hua Z, Ingram MF, Liu K, Chen SI, Graham TR. Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo. Curr. Biol. 2002 Sep 9/17/2002; 12(18): 1623-7. PMID: 12372257, PII: S096098220201148X, ISSN: 0960-9822.
Hua Z, Fatheddin P, Graham TR. An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system. Mol. Biol. Cell. 2002 Sep; 13(9): 3162-77. PMID: 12221123, PMCID: PMC124150, DOI: 10.1091/mbc.E02-03-0172, ISSN: 1059-1524.
Graham TR. Metabolic labeling and immunoprecipitation of yeast proteins. Curr Protoc Cell Biol. 2001 May; Chapter 7: Unit 7.6. PMID: 18228384, DOI: 10.1002/0471143030.cb0706s06, ISSN: 1934-2616.
Gall WE, Higginbotham MA, Chen C, Ingram MF, Cyr DM, Graham TR. The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast. Curr. Biol. 2000 Nov 11/2/2000; 10(21): 1349-58. PMID: 11084334, PII: S0960-9822(00)00771-5, ISSN: 0960-9822.
Gall WE, MA Higginbotham, C-Y Chen, MF Ingram, DM Cyr, and TR Graham. The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast. 2000.
Hopkins BD, Sato K, Nakano A, Graham TR. Introduction of Kex2 cleavage sites in fusion proteins for monitoring localization and transport in yeast secretory pathway. Meth. Enzymol. 2000; 327: 107-18. PMID: 11044978, PII: S0076-6879(00)27271-6, ISSN: 0076-6879.
Brigance WT, Barlowe C, Graham TR. Organization of the yeast Golgi complex into at least four functionally distinct compartments. Mol. Biol. Cell. 2000 Jan; 11(1): 171-82. PMID: 10637300, PMCID: PMC14766, ISSN: 1059-1524.
Chen CY, Ingram MF, Rosal PH, Graham TR. Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function. J. Cell Biol. 1999 Dec 12/13/1999; 147(6): 1223-36. PMID: 10601336, PMCID: PMC2168089, ISSN: 0021-9525.
Chen, C.-Y., M.F. Ingram, P. Rosal, and T.R. Graham. Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function. J. Cell Biol. 1999; 147: 1223-36.
Reynolds TB, Hopkins BD, Lyons MR, Graham TR. The high osmolarity glycerol response (HOG) MAP kinase pathway controls localization of a yeast golgi glycosyltransferase. J. Cell Biol. 1998 Nov 11/16/1998; 143(4): 935-46. PMID: 9817752, PMCID: PMC2132948, ISSN: 0021-9525.
Chen CY, Graham TR. An arf1Delta synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport in Saccharomyces cerevisiae. Genetics. 1998 Oct; 150(2): 577-89. PMID: 9755191, PMCID: PMC1460353, ISSN: 0016-6731.
Gaynor EC, Graham TR, Emr SD. COPI in ER/Golgi and intra-Golgi transport: do yeast COPI mutants point the way?. Biochim. Biophys. Acta. 1998 Aug 8/14/1998; 1404(1-2): 33-51. PMID: 9714721, PII: S0167-4889(98)00045-7, ISSN: 0006-3002.
Gaynor EC, Chen CY, Emr SD, Graham TR. ARF is required for maintenance of yeast Golgi and endosome structure and function. Mol. Biol. Cell. 1998 Mar; 9(3): 653-70. PMID: 9487133, PMCID: PMC25294, ISSN: 1059-1524.
Chen, C.-Y., and T. R. Graham. An arf1 synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport. Genetics. 1998; 150: 577-89.
Gaynor, E.C., T.R. Graham and S.D. Emr. COPI in ER/Golgi transport and intra-Golgi transport: do yeast COPs point the way?. Bioch. Biophys. Acta. 1998; 1404: 33-51.
Gaynor, E. C., C.-Y. Chen, S. D.Emr, and T. R.Graham. ARF is required for maintenance of yeast Golgi and endosome structure and function. Mol. Biol. Cell. 1998; 9: 653-70.
Reynolds, T.B., B.D. Hopkins, M.R. Lyons and T.R. Graham. The high osmolarity glycerol response (HOG) MAP kinase pathway controls localization of a yeast Golgi glycosyltransferase. J. Cell Biol. 1998; 143: 935-46.
Graham TR, Krasnov VA. Sorting of yeast alpha 1,3 mannosyltransferase is mediated by a lumenal domain interaction, and a transmembrane domain signal that can confer clathrin-dependent Golgi localization to a secreted protein. Mol. Biol. Cell. 1995 Jul; 6(7): 809-24. PMID: 7579696, PMCID: PMC301242, ISSN: 1059-1524.
Graham, T. R., and V. A. Krasnov. Sorting of yeast alpha1,3 mannosyltransferase is mediated by a lumenal domain interaction, and a transmembrane domain signal that can confer clathrin-dependent Golgi localization to a secreted protein. 1995.
Krasnov, V. and Graham, T.R. The Golgi complex of Saccharomyces cerevisiae. Can J Botany. 1995; 73: S343-S346.
Graham TR, Seeger M, Payne GS, MacKay VL, Emr SD. Clathrin-dependent localization of alpha 1,3 mannosyltransferase to the Golgi complex of Saccharomyces cerevisiae. J. Cell Biol. 1994 Nov; 127(3): 667-78. PMID: 7962051, PMCID: PMC2120240, ISSN: 0021-9525.
Graham, T.R., Seegar, M., MacKay, V., Payne, G.S., and Emr, S.D. Clathrin-dependent localization of a, 3-mannosyltransferase to the Golgi complex of Saccharomyces cerevisiae. J Cell Biology. 1994; 127: 667-78.
Gaynor, E.C., te Heesen, S., Graham, T.R., Aebi, M., and Emr, S.D. Signal-mediated retrieval of a membrane protein from the Golgi to the ER in yeast. J Cell Biology. 1994; 127: 653-65.
Graham, T.R. and S.D. Emr. SEC18. In: Guidebook to the Secretory Pathway (J. Rothblatt, P. Novick, and T. Stevens, eds.) Oxford Univ. Press, NY. 1994; 132-3.
Graham, T.R., Scott, P., and Emr, Scott D. Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway. EMBO J. 1993; 12: 869-77.
Horazdovsky, B.F., Graham, T.R., and Emr, S.D. ¿Vacuolar protein sorting in yeast". Protein Synthesis and Targeting in Yeast (M.F. Tuite, J.E.G. McCarthy, A.J. Brown and F. Sherman, eds.) Springer Verlag, Berlin. 1992.
Lacoste, H.C., Graham, T.R., and Kaplan, A. A sequence in b-hexosaminadase from Dictyostelium discoideum required for sorting of proteins to a compartment involved in developmentally induced secretion. J Biol Chem. 1992; 267: 5942-8.
Graham, T.R. and Emr, S.D. Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant. J Cell Biology. 1991; 114: 207-18.
Vida, T.A., Herman, P.K., Emr, S.D., and Graham, T.R. Compartmentalized transport, modification and sorting of yeast vacuolar hydrolases. Biomed. Biochem. Acta. 1991; 50: 413-20.
Robinson, J.S., Graham, T.R.,and Emr, S.D. A putative zinc finger protein, Saccaromyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient a factor prohormone maturation. Mol Cell Biol. 1991; 12: 5813-24.
Best JT, Xu P, Graham TR. Phospholipid flippases in membrane remodeling and transport carrier biogenesis. Curr. Opin. Cell Biol [print-electronic]. 2019 Mar 3/18/2019; 59: 8-15. PMID: 30897446, PII: S0955-0674(19)30001-8, DOI: 10.1016/j.ceb.2019.02.004, ISSN: 1879-0410.
Roland BP, Naito T, Best JT, Arnaiz-Yépez C, Takatsu H, Yu RJ, Shin HW, Graham TR. Yeast and human P4-ATPases transport glycosphingolipids using conserved structural motifs. J. Biol. Chem [print-electronic]. 2019 Feb 2/8/2019; 294(6): 1794-806. PMID: 30530492, PMCID: PMC6369285, PII: RA118.005876, DOI: 10.1074/jbc.RA118.005876, ISSN: 1083-351X.
Xu P, Hankins HM, MacDonald C, Erlinger SJ, Frazier MN, Diab NS, Piper RC, Jackson LP, MacGurn JA, Graham TR. COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal. Elife. 2017 Oct 10/23/2017; 6: PMID: 29058666, PMCID: PMC5663479, PII: 28342, DOI: 10.7554/eLife.28342, ISSN: 2050-084X.
van Leeuwen J, Pons C, Mellor JC, Yamaguchi TN, Friesen H, Koschwanez J, Ušaj MM, Pechlaner M, Takar M, Ušaj M, VanderSluis B, Andrusiak K, Bansal P, Baryshnikova A, Boone CE, Cao J, Cote A, Gebbia M, Horecka G, Horecka I, Kuzmin E, Legro N, Liang W, van Lieshout N, McNee M, San Luis BJ, Shaeri F, Shuteriqi E, Sun S, Yang L, Youn JY, Yuen M, Costanzo M, Gingras AC, Aloy P, Oostenbrink C, Murray A, Graham TR, Myers CL, Andrews BJ, Roth FP, Boone C. Exploring genetic suppression interactions on a global scale. Science. 2016 Nov 11/4/2016; 354(6312): PMID: 27811238, PII: 354/6312/aag0839, DOI: 10.1126/science.aag0839, ISSN: 1095-9203.
Roland BP, Graham TR. Directed evolution of a sphingomyelin flippase reveals mechanism of substrate backbone discrimination by a P4-ATPase. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2016 Aug 8/2/2016; 113(31): E4460-6. PMID: 27432949, PMCID: PMC4978280, PII: 1525730113, DOI: 10.1073/pnas.1525730113, ISSN: 1091-6490.
Takar M, Wu Y, Graham TR. The Essential Neo1 Protein from Budding Yeast Plays a Role in Establishing Aminophospholipid Asymmetry of the Plasma Membrane. J. Biol. Chem [print-electronic]. 2016 Jul 7/22/2016; 291(30): 15727-39. PMID: 27235400, PMCID: PMC4957055, PII: M115.686253, DOI: 10.1074/jbc.M115.686253, ISSN: 1083-351X.
Wu Y, Takar M, Cuentas-Condori AA, Graham TR. Neo1 and phosphatidylethanolamine contribute to vacuole membrane fusion in Saccharomyces cerevisiae. Cell Logist. 2016 Jul; 6(3): e1228791. PMID: 27738552, PMCID: PMC5058351, PII: 1228791, DOI: 10.1080/21592799.2016.1228791, ISSN: 2159-2780.
Roland BP, Graham TR. Decoding P4-ATPase substrate interactions. Crit. Rev. Biochem. Mol. Biol [print-electronic]. 2015 Dec; 51(6): 513-27. PMID: 27696908, PMCID: PMC5285478, DOI: 10.1080/10409238.2016.1237934, ISSN: 1549-7798.
Hankins HM, Sere YY, Diab NS, Menon AK, Graham TR. Phosphatidylserine translocation at the yeast trans-Golgi network regulates protein sorting into exocytic vesicles. Mol. Biol. Cell [print-electronic]. 2015 Dec 12/15/2015; 26(25): 4674-85. PMID: 26466678, PMCID: PMC4678023, PII: mbc.E15-07-0487, DOI: 10.1091/mbc.E15-07-0487, ISSN: 1939-4586.
Hankins HM, Baldridge RD, Xu P, Graham TR. Role of flippases, scramblases and transfer proteins in phosphatidylserine subcellular distribution. Traffic [print-electronic]. 2015 Jan; 16(1): 35-47. PMID: 25284293, PMCID: PMC4275391, DOI: 10.1111/tra.12233, ISSN: 1600-0854.
Zhou X, Sebastian TT, Graham TR. Auto-inhibition of Drs2p, a yeast phospholipid flippase, by its carboxyl-terminal tail. J. Biol. Chem [print-electronic]. 2013 Nov 11/1/2013; 288(44): 31807-15. PMID: 24045945, PMCID: PMC3814774, PII: M113.481986, DOI: 10.1074/jbc.M113.481986, ISSN: 1083-351X.
Xu P, Baldridge RD, Chi RJ, Burd CG, Graham TR. Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport. J. Cell Biol [print-electronic]. 2013 Sep 9/16/2013; 202(6): 875-86. PMID: 24019533, PMCID: PMC3776346, PII: jcb.201305094, DOI: 10.1083/jcb.201305094, ISSN: 1540-8140.
Baldridge RD, Xu P, Graham TR. Type IV P-type ATPases distinguish mono- versus diacyl phosphatidylserine using a cytofacial exit gate in the membrane domain. J. Biol. Chem [print-electronic]. 2013 Jul 7/5/2013; 288(27): 19516-27. PMID: 23709217, PMCID: PMC3707653, PII: M113.476911, DOI: 10.1074/jbc.M113.476911, ISSN: 1083-351X.
Graham TR. Arl1 gets into the membrane remodeling business with a flippase and ArfGEF. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Feb 2/19/2013; 110(8): 2691-2. PMID: 23401560, PMCID: PMC3581979, PII: 1300420110, DOI: 10.1073/pnas.1300420110, ISSN: 1091-6490.
Baldridge RD, Graham TR. Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Jan 1/29/2013; 110(5): E358-67. PMID: 23302692, PMCID: PMC3562821, PII: 1216948110, DOI: 10.1073/pnas.1216948110, ISSN: 1091-6490.
Sebastian TT, Baldridge RD, Xu P, Graham TR. Phospholipid flippases: building asymmetric membranes and transport vesicles. Biochim. Biophys. Acta [print-electronic]. 2012 Aug; 1821(8): 1068-77. PMID: 22234261, PMCID: PMC3368091, PII: S1388-1981(11)00272-1, DOI: 10.1016/j.bbalip.2011.12.007, ISSN: 0006-3002.
Baldridge RD, Graham TR. Identification of residues defining phospholipid flippase substrate specificity of type IV P-type ATPases. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Feb 2/7/2012; 109(6): E290-8. PMID: 22308393, PMCID: PMC3277569, PII: 1115725109, DOI: 10.1073/pnas.1115725109, ISSN: 1091-6490.
Graham TR, Burd CG. Coordination of Golgi functions by phosphatidylinositol 4-kinases. Trends Cell Biol [print-electronic]. 2011 Feb; 21(2): 113-21. PMID: 21282087, PMCID: PMC3053015, PII: S0962-8924(10)00214-X, DOI: 10.1016/j.tcb.2010.10.002, ISSN: 1879-3088.
Graham TR, Kozlov MM. Interplay of proteins and lipids in generating membrane curvature. Curr. Opin. Cell Biol [print-electronic]. 2010 Aug; 22(4): 430-6. PMID: 20605711, PMCID: PMC3770468, PII: S0955-0674(10)00065-7, DOI: 10.1016/j.ceb.2010.05.002, ISSN: 1879-0410.
Natarajan P, Liu K, Patil DV, Sciorra VA, Jackson CL, Graham TR. Regulation of a Golgi flippase by phosphoinositides and an ArfGEF. Nat. Cell Biol [print-electronic]. 2009 Dec; 11(12): 1421-6. PMID: 19898464, PMCID: PMC2787759, PII: ncb1989, DOI: 10.1038/ncb1989, ISSN: 1476-4679.
Zhou X, Graham TR. Reconstitution of phospholipid translocase activity with purified Drs2p, a type-IV P-type ATPase from budding yeast. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2009 Sep 9/29/2009; 106(39): 16586-91. PMID: 19805341, PMCID: PMC2757829, PII: 0904293106, DOI: 10.1073/pnas.0904293106, ISSN: 1091-6490.
Muthusamy BP, Natarajan P, Zhou X, Graham TR. Linking phospholipid flippases to vesicle-mediated protein transport. Biochim. Biophys. Acta [print-electronic]. 2009 Jul; 1791(7): 612-9. PMID: 19286470, PMCID: PMC3770137, PII: S1388-1981(09)00072-9, DOI: 10.1016/j.bbalip.2009.03.004, ISSN: 0006-3002.
Muthusamy BP, Raychaudhuri S, Natarajan P, Abe F, Liu K, Prinz WA, Graham TR. Control of protein and sterol trafficking by antagonistic activities of a type IV P-type ATPase and oxysterol binding protein homologue. Mol. Biol. Cell [print-electronic]. 2009 Jun; 20(12): 2920-31. PMID: 19403696, PMCID: PMC2695799, PII: E08-10-1036, DOI: 10.1091/mbc.E08-10-1036, ISSN: 1939-4586.
Ho CH, Magtanong L, Barker SL, Gresham D, Nishimura S, Natarajan P, Koh JLY, Porter J, Gray CA, Andersen RJ, Giaever G, Nislow C, Andrews B, Botstein D, Graham TR, Yoshida M, Boone C. A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds. Nat. Biotechnol [print-electronic]. 2009 Apr; 27(4): 369-77. PMID: 19349972, PMCID: PMC3856559, DOI: 10.1038/nbt.1534, ISSN: 1546-1696.
Ho CH, Magtanong L, Barker SL, Gresham D, Nishimura S, Natarajan P, Koh JLY, Porter J, Gray CA, Andersen RJ, Giaever G, Nislow C, Andrews B, Botstein D, Graham TR, Yoshida M, Boone C. A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds. Nat. Biotechnol [print-electronic]. 2009 Apr; 27(4): 369-77. PMID: 19349972, PMCID: PMC3856559, DOI: 10.1038/nbt.1534, ISSN: 1546-1696.
Liu K, Surendhran K, Nothwehr SF, Graham TR. P4-ATPase requirement for AP-1/clathrin function in protein transport from the trans-Golgi network and early endosomes. Mol. Biol. Cell [print-electronic]. 2008 Aug; 19(8): 3526-35. PMID: 18508916, PMCID: PMC2488278, PII: E08-01-0025, DOI: 10.1091/mbc.E08-01-0025, ISSN: 1939-4586.
Liu K, Hua Z, Nepute JA, Graham TR. Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Mol. Biol. Cell [print-electronic]. 2007 Feb; 18(2): 487-500. PMID: 17122361, PMCID: PMC1783782, PII: E06-07-0592, DOI: 10.1091/mbc.E06-07-0592, ISSN: 1059-1524.
Chen S, Wang J, Muthusamy BP, Liu K, Zare S, Andersen RJ, Graham TR. Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane. Traffic [print-electronic]. 2006 Nov; 7(11): 1503-17. PMID: 16956384, PII: TRA485, DOI: 10.1111/j.1600-0854.2006.00485.x, ISSN: 1398-9219.
Parsons AB, Lopez A, Givoni IE, Williams DE, Gray CA, Porter J, Chua G, Sopko R, Brost RL, Ho CH, Wang J, Ketela T, Brenner C, Brill JA, Fernandez GE, Lorenz TC, Payne GS, Ishihara S, Ohya Y, Andrews B, Hughes TR, Frey BJ, Graham TR, Andersen RJ, Boone C. Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast. Cell. 2006 Aug 8/11/2006; 126(3): 611-25. PMID: 16901791, PII: S0092-8674(06)00906-8, DOI: 10.1016/j.cell.2006.06.040, ISSN: 0092-8674.
Xiao J, Kim LS, Graham TR. Dissection of Swa2p/auxilin domain requirements for cochaperoning Hsp70 clathrin-uncoating activity in vivo. Mol. Biol. Cell [print-electronic]. 2006 Jul; 17(7): 3281-90. PMID: 16687570, PMCID: PMC1483056, PII: E06-02-0106, DOI: 10.1091/mbc.E06-02-0106, ISSN: 1059-1524.
Natarajan P, Graham TR. Measuring translocation of fluorescent lipid derivatives across yeast Golgi membranes. Methods. 2006 Jun; 39(2): 163-8. PMID: 16828307, PII: S1046-2023(06)00080-6, DOI: 10.1016/j.ymeth.2006.05.009, ISSN: 1046-2023.
Graham TR. Flippases and vesicle-mediated protein transport. Trends Cell Biol. 2004 Dec; 14(12): 670-7. PMID: 15564043, PII: S0962-8924(04)00287-9, DOI: 10.1016/j.tcb.2004.10.008, ISSN: 0962-8924.
Natarajan P, Wang J, Hua Z, Graham TR. Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2004 Jul 7/20/2004; 101(29): 10614-9. PMID: 15249668, PMCID: PMC489982, PII: 0404146101, DOI: 10.1073/pnas.0404146101, ISSN: 0027-8424.
Graham TR. Membrane targeting: getting Arl to the Golgi. Curr. Biol. 2004 Jun 6/22/2004; 14(12): R483-5. PMID: 15203023, PII: S096098220400421X, DOI: 10.1016/j.cub.2004.06.017, ISSN: 0960-9822.
Chim N, Gall WE, Xiao J, Harris MP, Graham TR, Krezel AM. Solution structure of the ubiquitin-binding domain in Swa2p from Saccharomyces cerevisiae. Proteins. 2004 Mar 3/1/2004; 54(4): 784-93. PMID: 14997574, DOI: 10.1002/prot.10636, ISSN: 1097-0134.
Chantalat S, Park SK, Hua Z, Liu K, Gobin R, Peyroche A, Rambourg A, Graham TR, Jackson CL. The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae. J. Cell. Sci [print-electronic]. 2004 Feb 2/15/2004; 117(Pt 5): 711-22. PMID: 14734650, PII: jcs.00896, DOI: 10.1242/jcs.00896, ISSN: 0021-9533.
Hua Z, Graham TR. Requirement for neo1p in retrograde transport from the Golgi complex to the endoplasmic reticulum. Mol. Biol. Cell [print-electronic]. 2003 Dec; 14(12): 4971-83. PMID: 12960419, PMCID: PMC284799, PII: E03-07-0463, DOI: 10.1091/mbc.E03-07-0463, ISSN: 1059-1524.
Gall WE, Geething NC, Hua Z, Ingram MF, Liu K, Chen SI, Graham TR. Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo. Curr. Biol. 2002 Sep 9/17/2002; 12(18): 1623-7. PMID: 12372257, PII: S096098220201148X, ISSN: 0960-9822.
Hua Z, Fatheddin P, Graham TR. An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system. Mol. Biol. Cell. 2002 Sep; 13(9): 3162-77. PMID: 12221123, PMCID: PMC124150, DOI: 10.1091/mbc.E02-03-0172, ISSN: 1059-1524.
Graham TR. Metabolic labeling and immunoprecipitation of yeast proteins. Curr Protoc Cell Biol. 2001 May; Chapter 7: Unit 7.6. PMID: 18228384, DOI: 10.1002/0471143030.cb0706s06, ISSN: 1934-2616.
Gall WE, Higginbotham MA, Chen C, Ingram MF, Cyr DM, Graham TR. The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast. Curr. Biol. 2000 Nov 11/2/2000; 10(21): 1349-58. PMID: 11084334, PII: S0960-9822(00)00771-5, ISSN: 0960-9822.
Gall WE, MA Higginbotham, C-Y Chen, MF Ingram, DM Cyr, and TR Graham. The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast. 2000.
Hopkins BD, Sato K, Nakano A, Graham TR. Introduction of Kex2 cleavage sites in fusion proteins for monitoring localization and transport in yeast secretory pathway. Meth. Enzymol. 2000; 327: 107-18. PMID: 11044978, PII: S0076-6879(00)27271-6, ISSN: 0076-6879.
Brigance WT, Barlowe C, Graham TR. Organization of the yeast Golgi complex into at least four functionally distinct compartments. Mol. Biol. Cell. 2000 Jan; 11(1): 171-82. PMID: 10637300, PMCID: PMC14766, ISSN: 1059-1524.
Chen CY, Ingram MF, Rosal PH, Graham TR. Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function. J. Cell Biol. 1999 Dec 12/13/1999; 147(6): 1223-36. PMID: 10601336, PMCID: PMC2168089, ISSN: 0021-9525.
Chen, C.-Y., M.F. Ingram, P. Rosal, and T.R. Graham. Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function. J. Cell Biol. 1999; 147: 1223-36.
Reynolds TB, Hopkins BD, Lyons MR, Graham TR. The high osmolarity glycerol response (HOG) MAP kinase pathway controls localization of a yeast golgi glycosyltransferase. J. Cell Biol. 1998 Nov 11/16/1998; 143(4): 935-46. PMID: 9817752, PMCID: PMC2132948, ISSN: 0021-9525.
Chen CY, Graham TR. An arf1Delta synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport in Saccharomyces cerevisiae. Genetics. 1998 Oct; 150(2): 577-89. PMID: 9755191, PMCID: PMC1460353, ISSN: 0016-6731.
Gaynor EC, Graham TR, Emr SD. COPI in ER/Golgi and intra-Golgi transport: do yeast COPI mutants point the way?. Biochim. Biophys. Acta. 1998 Aug 8/14/1998; 1404(1-2): 33-51. PMID: 9714721, PII: S0167-4889(98)00045-7, ISSN: 0006-3002.
Gaynor EC, Chen CY, Emr SD, Graham TR. ARF is required for maintenance of yeast Golgi and endosome structure and function. Mol. Biol. Cell. 1998 Mar; 9(3): 653-70. PMID: 9487133, PMCID: PMC25294, ISSN: 1059-1524.
Chen, C.-Y., and T. R. Graham. An arf1 synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport. Genetics. 1998; 150: 577-89.
Gaynor, E.C., T.R. Graham and S.D. Emr. COPI in ER/Golgi transport and intra-Golgi transport: do yeast COPs point the way?. Bioch. Biophys. Acta. 1998; 1404: 33-51.
Gaynor, E. C., C.-Y. Chen, S. D.Emr, and T. R.Graham. ARF is required for maintenance of yeast Golgi and endosome structure and function. Mol. Biol. Cell. 1998; 9: 653-70.
Reynolds, T.B., B.D. Hopkins, M.R. Lyons and T.R. Graham. The high osmolarity glycerol response (HOG) MAP kinase pathway controls localization of a yeast Golgi glycosyltransferase. J. Cell Biol. 1998; 143: 935-46.
Graham TR, Krasnov VA. Sorting of yeast alpha 1,3 mannosyltransferase is mediated by a lumenal domain interaction, and a transmembrane domain signal that can confer clathrin-dependent Golgi localization to a secreted protein. Mol. Biol. Cell. 1995 Jul; 6(7): 809-24. PMID: 7579696, PMCID: PMC301242, ISSN: 1059-1524.
Graham, T. R., and V. A. Krasnov. Sorting of yeast alpha1,3 mannosyltransferase is mediated by a lumenal domain interaction, and a transmembrane domain signal that can confer clathrin-dependent Golgi localization to a secreted protein. 1995.
Krasnov, V. and Graham, T.R. The Golgi complex of Saccharomyces cerevisiae. Can J Botany. 1995; 73: S343-S346.
Graham TR, Seeger M, Payne GS, MacKay VL, Emr SD. Clathrin-dependent localization of alpha 1,3 mannosyltransferase to the Golgi complex of Saccharomyces cerevisiae. J. Cell Biol. 1994 Nov; 127(3): 667-78. PMID: 7962051, PMCID: PMC2120240, ISSN: 0021-9525.
Graham, T.R., Seegar, M., MacKay, V., Payne, G.S., and Emr, S.D. Clathrin-dependent localization of a, 3-mannosyltransferase to the Golgi complex of Saccharomyces cerevisiae. J Cell Biology. 1994; 127: 667-78.
Gaynor, E.C., te Heesen, S., Graham, T.R., Aebi, M., and Emr, S.D. Signal-mediated retrieval of a membrane protein from the Golgi to the ER in yeast. J Cell Biology. 1994; 127: 653-65.
Graham, T.R. and S.D. Emr. SEC18. In: Guidebook to the Secretory Pathway (J. Rothblatt, P. Novick, and T. Stevens, eds.) Oxford Univ. Press, NY. 1994; 132-3.
Graham, T.R., Scott, P., and Emr, Scott D. Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway. EMBO J. 1993; 12: 869-77.
Horazdovsky, B.F., Graham, T.R., and Emr, S.D. ¿Vacuolar protein sorting in yeast". Protein Synthesis and Targeting in Yeast (M.F. Tuite, J.E.G. McCarthy, A.J. Brown and F. Sherman, eds.) Springer Verlag, Berlin. 1992.
Lacoste, H.C., Graham, T.R., and Kaplan, A. A sequence in b-hexosaminadase from Dictyostelium discoideum required for sorting of proteins to a compartment involved in developmentally induced secretion. J Biol Chem. 1992; 267: 5942-8.
Graham, T.R. and Emr, S.D. Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant. J Cell Biology. 1991; 114: 207-18.
Vida, T.A., Herman, P.K., Emr, S.D., and Graham, T.R. Compartmentalized transport, modification and sorting of yeast vacuolar hydrolases. Biomed. Biochem. Acta. 1991; 50: 413-20.
Robinson, J.S., Graham, T.R.,and Emr, S.D. A putative zinc finger protein, Saccaromyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient a factor prohormone maturation. Mol Cell Biol. 1991; 12: 5813-24.
Available Postdoctoral Position Details
Posted: 8/18/2017
Postdoctoral position available starting October of 2017 to the study structure/function relationships for a group of type IV P-type ATPases essential for membrane biogenesis and protein trafficking. These proteins are also implicated in neurological and metabolic diseases in man.