Richard L. Hoover, Ph.D.

Professor

richard.hoover@vanderbilt.edu
Faculty Appointments
Associate Dean for Academic Affairs Professor of Pathology, Microbiology and Immunology Associate Professor of Pediatrics
Education
Ph.D., Cell Biology, Michigan State University, East Lansing, MichiganM.S., University of Kentucky, Lexington, KentuckyB.A., Ohio State University, Columbus, Ohio
Office Address
U-2216 MCN
AL 2561
Research Description
Research in this laboratory employs in vitro model systems to investigate the roles of interactions between leukocytes and the endothelial lining of blood vessels in acute inflammation and atherosclerosis. Current work is directed at the responses of endothelial cells to physical contact with leukocytes and the resulting effects on synthesis and secretion of growth factors, arachidonic acid metabolites, plasminogen activators, metalloproteinases, and other endothelial cell adhesion molecules.

Signal transduction pathways that are activated as a result of the cell-cell interactions are being examined to determine their contributions. Other aspects of the work concern the polarity of endothelial cells and the topography of endothelial cell function. We are also investigating differences in the biology of endothelial cells isolated from different blood vessel types, such as large vessel aortic endothelium versus small vessel capillary endothelium, in an attempt to correlate cell function with vessel structure.
Research Keywords
Cell-cell interactions emphasizing leukocyte-endothelial systems; endothelial cell biology
Publications
Tilghman RW, Hoover RL. E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells. FEBS Lett. 2002 Aug 8/14/2002; 525(1-3): 83-7. PMID: 12163166, PII: S0014579302030703, ISSN: 0014-5793.

Tilghman RW, Hoover RL. The Src-cortactin pathway is required for clustering of E-selectin and ICAM-1 in endothelial cells. FASEB J [print-electronic]. 2002 Aug; 16(10): 1257-9. PMID: 12060669, PII: 01-0969fje, DOI: 10.1096/fj.01-0969fje, ISSN: 1530-6860.

Pekovich SR, Bock PE, Hoover RL. Thrombin-thrombomodulin activation of protein C facilitates the activation of progelatinase A. FEBS Lett. 2001 Apr 4/6/2001; 494(1-2): 129-32. PMID: 11297749, PII: S0014-5793(01)02296-7, ISSN: 0014-5793.

Haralson MA, DiMari SJ, Hoover RL, Harris RC. Effects of epidermal growth factor on collagen expression by rat kidney mesangial cells in culture. Matrix Biol. 2000 Feb; 19(1): 47-59. PMID: 10686425, PII: S0945-053X(99)00057-8, ISSN: 0945-053X.

Matsuo K, Ikizler TA, Hoover RL, Nakamoto M, Yasunaga C, Pupim LB, Hakim RM. Transforming growth factor-beta is involved in the pathogenesis of dialysis-related amyloidosis. Kidney Int. 2000 Feb; 57(2): 697-708. PMID: 10652049, PII: kid892, DOI: 10.1046/j.1523-1755.2000.00892.x, ISSN: 0085-2538.

Amorino, G.P. and Hoover, R.L. Interactions of monocytes with human endothelial cells stimulate monocyte metallo proteinase production. 1998.

Amorino GP, Hoover RL. Interactions of monocytic cells with human endothelial cells stimulate monocytic metalloproteinase production. Am. J. Pathol. 1998 Jan; 152(1): 199-207. PMID: 9422537, PMCID: PMC1858121, ISSN: 0002-9440.

Klekamp JG, Jarzecka K, Hoover RL, Summar ML, Redmond N, Perkett EA. Vascular endothelial growth factor is expressed in ovine pulmonary vascular smooth muscle cells in vitro and regulated by hypoxia and dexamethasone. Pediatr. Res. 1997 Dec; 42(6): 744-9. PMID: 9396552, DOI: 10.1203/00006450-199712000-00005, ISSN: 0031-3998.

Inagami, T., Naruse, M., Hoover, R. Endothelium as an endocrine organ. 1995.

Kells, A. F., Coates, S. R., Schwartz, H. S., Hoover, R. L. TGFb and PDGF act synergistically in affecting the growth of human osteoblast-enriched cultures. 1995.

Morello, J. P., Plamondon, J., Meyrick, B., Hoover, R. L., O'Conner-McCourt, M. D. Transforming growth factor-b receptor expression on endothelial cells; heterogeneity of type III receptor. 1995.

Kells AF, Coats SR, Schwartz HS, Hoover RL. TGF-beta and PDGF act synergistically in affecting the growth of human osteoblast-enriched cultures. Connect. Tissue Res. 1995; 31(2): 117-24. PMID: 15612327, ISSN: 0300-8207.

Yoshioka T, Kawamura T, Meyrick BO, Beckman JK, Hoover RL, Yoshida H, Ichikawa I. Induction of manganese superoxide dismutase by glucocorticoids in glomerular cells. Kidney Int. 1994 Jan; 45(1): 211-9. PMID: 8127010, ISSN: 0085-2538.

Takeda M, Homma T, Breyer MD, Horiba N, Hoover RL, Kawamoto S, Ichikawa I, Kon V. Volume and agonist-induced regulation of myosin light-chain phosphorylation in glomerular mesangial cells. Am. J. Physiol. 1993 Mar; 264(3 Pt 2): F421-6. PMID: 8456955, ISSN: 0002-9513.

Awazu M, Yared A, Swift LL, Hoover RL, Ichikawa I. Dietary fatty acid modulates glomerular atrial natriuretic peptide receptor. Kidney Int. 1992 Aug; 42(2): 265-71. PMID: 1328748, ISSN: 0085-2538.

Takahashi K, Nammour TM, Fukunaga M, Ebert J, Morrow JD, Roberts LJ, Hoover RL, Badr KF. Glomerular actions of a free radical-generated novel prostaglandin, 8-epi-prostaglandin F2 alpha, in the rat. Evidence for interaction with thromboxane A2 receptors. J. Clin. Invest. 1992 Jul; 90(1): 136-41. PMID: 1386085, PMCID: PMC443072, DOI: 10.1172/JCI115826, ISSN: 0021-9738.

Garcia FU, Wojta J, Hoover RL. Interactions between live Bartonella bacilliformis and endothelial cells. J. Infect. Dis. 1992 Jun; 165(6): 1138-41. PMID: 1583335, ISSN: 0022-1899.

Takeda M, Breyer MD, Noland TD, Homma T, Hoover RL, Inagami T, Kon V. Endothelin-1 receptor antagonist: effects on endothelin- and cyclosporine-treated mesangial cells. Kidney Int. 1992 Jun; 41(6): 1713-9. PMID: 1323728, ISSN: 0085-2538.

Gilcrease MZ, Hoover RL. Secretion of a chemotactic substance(s) by AGE-stimulated human monocytes. Diabetes Res. Clin. Pract. 1992 Apr; 16(1): 7-11. PMID: 1576934, ISSN: 0168-8227.

Makita N, Funk CD, Imai E, Hoover RL, Badr KF. Molecular cloning and functional expression of rat leukotriene A4 hydrolase using the polymerase chain reaction. FEBS Lett. 1992 Mar 3/16/1992; 299(3): 273-7. PMID: 1544505, ISSN: 0014-5793.

Hill KE, Hunt RW, Jones R, Hoover RL, Burk RF. Metabolism of nitroglycerin by smooth muscle cells. Involvement of glutathione and glutathione S-transferase. Biochem. Pharmacol. 1992 Feb 2/4/1992; 43(3): 561-6. PMID: 1540213, PII: 0006-2952(92)90579-8, ISSN: 0006-2952.

Gilcrease MZ, Hoover RL. Human monocyte interactions with non-enzymatically glycated collagen. Diabetologia. 1992 Feb; 35(2): 160-4. PMID: 1547921, ISSN: 0012-186X.

Kells AF, Schwartz HS, Bascom CC, Hoover RL. Identification and analysis of transforming growth factor beta receptors on primary osteoblast-enriched cultures derived from adult human bone. Connect. Tissue Res. 1992; 27(4): 197-209. PMID: 1315659, ISSN: 0300-8207.

Hiraoka-Yoshimoto M, Higashida K, Takeda M, Kawamoto S, Ichikawa I, Hoover RL. Characterization of myosin heavy and light chains in cultured mesangial cells. Kidney Int. 1991 Dec; 40(6): 1013-9. PMID: 1762302, ISSN: 0085-2538.

Gilcrease MZ, Hoover RL. Examination of monocyte adherence to endothelium under hyperglycemic conditions. Am. J. Pathol. 1991 Nov; 139(5): 1089-97. PMID: 1835304, PMCID: PMC1886347, ISSN: 0002-9440.

Gilcrease MZ, Hoover RL. Neutrophil adhesion to endothelium following hyperosmolar insult. Diabetes Res. 1991 Apr; 16(4): 149-57. PMID: 1802480, ISSN: 0265-5985.

Broadley, C., Dawidowicz, E., Chong, P.L-G., and Hoover, R.L. Modulation of membrane cholesterol levels: effects on endothelial cell function. 1991.

Kon V, Sugiura M, Inagami T, Harvie BR, Ichikawa I, Hoover RL. Role of endothelin in cyclosporine-induced glomerular dysfunction. Kidney Int. 1990 Jun; 37(6): 1487-91. PMID: 2194067, ISSN: 0085-2538.

Gilcrease MZ, Hoover RL. Activated human monocytes exhibit receptor-mediated adhesion to a non-enzymatically glycosylated protein substrate. Diabetologia. 1990 Jun; 33(6): 329-33. PMID: 2165945, ISSN: 0012-186X.

Garcia FU, Wojta J, Broadley KN, Davidson JM, Hoover RL. Bartonella bacilliformis stimulates endothelial cells in vitro and is angiogenic in vivo. Am. J. Pathol. 1990 May; 136(5): 1125-35. PMID: 1693472, PMCID: PMC1877437, ISSN: 0002-9440.

Babbitt DG, Forman MB, Jones R, Bajaj AK, Hoover RL. Prevention of neutrophil-mediated injury to endothelial cells by perfluorochemical. Am. J. Pathol. 1990 Feb; 136(2): 451-9. PMID: 2407126, PMCID: PMC1877412, ISSN: 0002-9440.

Broadley C, Hoover RL. Ceruloplasmin reduces the adhesion and scavenges superoxide during the interaction of activated polymorphonuclear leukocytes with endothelial cells. Am. J. Pathol. 1989 Oct; 135(4): 647-55. PMID: 2552811, PMCID: PMC1880036, ISSN: 0002-9440.

Atkinson JB, Hoover RL, Berry KK, Swift LL. Cholesterol-fed heterozygous Watanabe heritable hyperlipidemic rabbits: a new model for atherosclerosis. Atherosclerosis. 1989 Aug; 78(2-3): 123-36. PMID: 2783197, PII: 0021-9150(89)90216-5, ISSN: 0021-9150.

Badr KF, Mong S, Hoover RL, Schwartzberg M, Ebert J, Jacobson HR, Harris RC. Leukotriene D4 binding and signal transduction in rat glomerular mesangial cells. Am. J. Physiol. 1989 Aug; 257(2 Pt 2): F280-7. PMID: 2548402, ISSN: 0002-9513.

Wojta J, Binder BR, Huber K, Hoover RL. Evaluation of fibrinolytic capacity in plasma during thrombolytic therapy with single (scu-PA) or two-chain urokinase type plasminogen activator (tcu-PA) by a combined assay system for urokinase type plasminogen activator antigen and function. Thromb. Haemost. 1989 Apr 4/25/1989; 61(2): 289-93. PMID: 2501899, ISSN: 0340-6245.

Wojta J, Hoover RL, Daniel TO. Vascular origin determines plasminogen activator expression in human endothelial cells. Renal endothelial cells produce large amounts of single chain urokinase type plasminogen activator. J. Biol. Chem. 1989 Feb 2/15/1989; 264(5): 2846-52. PMID: 2492525, ISSN: 0021-9258.

Wojta, J., Hoover, R. L, Daniel, T. O. Vascular origin determines plasinogen activator expression in human endothelial cells: renal endothelial cells produce large amounts of scu-PA?. 1989.

Wojta J, Jones RL, Binder BR, Hoover RL. Reduction in pO2 decreases the fibrinolytic potential of cultured bovine endothelial cells derived from pulmonary arteries and lung microvasculature. Blood. 1988 Jun; 71(6): 1703-6. PMID: 3259442, ISSN: 0006-4971.

Haralson MA, Jacobson HR, Hoover RL. Collagen polymorphism in cultured rat kidney mesangial cells. Lab. Invest. 1987 Nov; 57(5): 513-23. PMID: 3682763, ISSN: 0023-6837.