H. Charles Manning, Ph.D.



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Faculty Appointments
Professor of Radiology and Radiological Sciences Ingram Associate Professor of Cancer ResearchProfessor of ChemistryProfessor of Biomedical EngineeringProfessor in Neurological Surgery
Ph.D., Chemistry, Texas Tech University, Lubbock, TexasB.Sc., Chemistry, Tarleton State University, Stephenville, Texas
Office Address
1161 21st Avenue South
MCN AA-1105
Nashville, TN 37232-2310
Research Description
Our multi-disciplinary research program involves the development, validation, and translation of non-invasive molecular imaging metrics to assess disease progression and response to therapy. Projects within our program emphasize molecular imaging of cancer, and notably imaging-based assessment of response to anti-cancer pharmaceuticals in colorectal and breast cancer. We are actively engaged in advancing novel methods to image molecular events in both humans and animal models that include receptor expression, proliferation, angiogenesis, and apoptosis. Among these methods include validation of imaging probes for novel applications, as well as high-throughput small-molecule discovery to develop novel imaging compounds.

Section: Vanderbilt University Institute of Imaging Science
Research Keywords
Molecular imaging, chemistry, small-molecule discovery, therapeutic efficacy screening, clinical trial modeling, imaging probe development, imaging validation
Li J, Smith JA, Dawson ES, Fu A, Nickels ML, Schulte ML, Manning HC. Optimized Translocator Protein Ligand for Optical Molecular Imaging and Screening. Bioconjug. Chem [print-electronic]. 2017 Apr 4/19/2017; 28(4): 1016-23. PMID: 28156095, DOI: 10.1021/acs.bioconjchem.6b00711, ISSN: 1520-4812.

Xu J, Li K, Smith RA, Waterton JC, Zhao P, Ding Z, Does MD, Manning HC, Gore JC. A comparative assessment of preclinical chemotherapeutic response of tumors using quantitative non-Gaussian diffusion MRI. Magn Reson Imaging [print-electronic]. 2017 Apr; 37: 195-202. PMID: 27919785, PMCID: PMC5316364, PII: S0730-725X(16)30239-9, DOI: 10.1016/j.mri.2016.12.002, ISSN: 1873-5894.

Wang Y, Ayres KL, Goldman DA, Dickler MN, Bardia A, Mayer IA, Winer E, Fredrickson J, Arteaga CL, Baselga J, Manning HC, Mahmood U, Ulaner GA. (18)F-Fluoroestradiol PET/CT Measurement of Estrogen Receptor Suppression during a Phase I Trial of the Novel Estrogen Receptor-Targeted Therapeutic GDC-0810: Using an Imaging Biomarker to Guide Drug Dosage in Subsequent Trials. Clin. Cancer Res [print-electronic]. 2016 Dec 12/23/2016; PMID: 28011460, PII: 1078-0432.CCR-16-2197, DOI: 10.1158/1078-0432.CCR-16-2197, ISSN: 1078-0432.

Kavanaugh G, Williams J, Morris AS, Nickels ML, Walker R, Koglin N, Stephens AW, Washington MK, Geevarghese SK, Liu Q, Ayers D, Shyr Y, Manning HC. Utility of [(18)F]FSPG PET to Image Hepatocellular Carcinoma: First Clinical Evaluation in a US Population. Mol Imaging Biol. 2016 Dec; 18(6): 924-34. PMID: 27677886, PII: 10.1007/s11307-016-1007-0, DOI: 10.1007/s11307-016-1007-0, ISSN: 1860-2002.

Tang D, Li J, Buck JR, Tantawy MN, Xia Y, Harp JM, Nickels ML, Meiler J, Manning HC. Evaluation of TSPO PET Ligands [(18)F]VUIIS1009A and [(18)F]VUIIS1009B: Tracers for Cancer Imaging. Mol Imaging Biol [print-electronic]. 2016 Nov 11/16/2016; PMID: 27853987, PII: 10.1007/s11307-016-1027-9, DOI: 10.1007/s11307-016-1027-9, ISSN: 1860-2002.

Schulte ML, Hight MR, Ayers GD, Liu Q, Shyr Y, Washington MK, Manning HC. Non-Invasive Glutamine PET Reflects Pharmacological Inhibition of BRAF(V600E) In Vivo. Mol Imaging Biol [print-electronic]. 2016 Oct 10/21/2016; PMID: 27770401, PII: 10.1007/s11307-016-1008-z, DOI: 10.1007/s11307-016-1008-z, ISSN: 1860-2002.

Osgood CL, Tantawy MN, Maloney N, Madaj ZB, Peck A, Boguslawski E, Jess J, Buck J, Winn ME, Manning HC, Grohar PJ. (18)F-FLT Positron Emission Tomography (PET) is a Pharmacodynamic Marker for EWS-FLI1 Activity and Ewing Sarcoma. Sci Rep. 2016 Sep 9/27/2016; 6: 33926. PMID: 27671553, PMCID: PMC5037393, PII: srep33926, DOI: 10.1038/srep33926, ISSN: 2045-2322.

Qiao J, Grabowska MM, Forestier-Roman IS, Mirosevich J, Case TC, Chung DH, Cates JM, Matusik RJ, Manning HC, Jin R. Activation of GRP/GRP-R signaling contributes to castration-resistant prostate cancer progression. Oncotarget. 2016 Sep 9/20/2016; 7(38): 61955-69. PMID: 27542219, PMCID: PMC5308703, PII: 11326, DOI: 10.18632/oncotarget.11326, ISSN: 1949-2553.

Li J, Schulte ML, Nickels ML, Manning HC. New structure-activity relationships of N-acetamide substituted pyrazolopyrimidines as pharmacological ligands of TSPO. Bioorg. Med. Chem. Lett [print-electronic]. 2016 Aug 8/1/2016; 26(15): 3472-7. PMID: 27353534, PMCID: PMC4993599, PII: S0960-894X(16)30652-7, DOI: 10.1016/j.bmcl.2016.06.041, ISSN: 1464-3405.

Joseph JD, Darimont B, Zhou W, Arrazate A, Young A, Ingalla E, Walter K, Blake RA, Nonomiya J, Guan Z, Kategaya L, Govek SP, Lai AG, Kahraman M, Brigham D, Sensintaffar J, Lu N, Shao G, Qian J, Grillot K, Moon M, Prudente R, Bischoff E, Lee KJ, Bonnefous C, Douglas KL, Julien JD, Nagasawa JY, Aparicio A, Kaufman J, Haley B, Giltnane JM, Wertz IE, Lackner MR, Nannini MA, Sampath D, Schwarz L, Manning HC, Tantawy MN, Arteaga CL, Heyman RA, Rix PJ, Friedman L, Smith ND, Metcalfe C, Hager JH. The selective estrogen receptor downregulator GDC-0810 is efficacious in diverse models of ER+ breast cancer. Elife. 2016 Jul 7/13/2016; 5: PMID: 27410477, PMCID: PMC4961458, DOI: 10.7554/eLife.15828, ISSN: 2050-084X.

Manning HC, Buck JR, Cook RS. Mouse Models of Breast Cancer: Platforms for Discovering Precision Imaging Diagnostics and Future Cancer Medicine. J. Nucl. Med. 2016 Feb; 57 Suppl 1: 60S-8S. PMID: 26834104, PII: jnumed.115.157917, DOI: 10.2967/jnumed.115.157917, ISSN: 1535-5667.

Schulte ML, Khodadadi AB, Cuthbertson ML, Smith JA, Manning HC. 2-Amino-4-bis(aryloxybenzyl)aminobutanoic acids: A novel scaffold for inhibition of ASCT2-mediated glutamine transport. Bioorg. Med. Chem. Lett [print-electronic]. 2016 Feb 2/1/2016; 26(3): 1044-7. PMID: 26750251, PMCID: PMC4727990, PII: S0960-894X(15)30345-0, DOI: 10.1016/j.bmcl.2015.12.031, ISSN: 1464-3405.

Higginbotham JN, Zhang Q, Jeppesen DK, Scott AM, Manning HC, Ochieng J, Franklin JL, Coffey RJ. Identification and characterization of EGF receptor in individual exosomes by fluorescence-activated vesicle sorting. J Extracell Vesicles. 2016; 5: 29254. PMID: 27345057, PMCID: PMC4921784, PII: 29254.

Han W, Zaynagetdinov R, Yull FE, Polosukhin VV, Gleaves LA, Tanjore H, Young LR, Peterson TE, Manning HC, Prince LS, Blackwell TS. Molecular imaging of folate receptor ß-positive macrophages during acute lung inflammation. Am. J. Respir. Cell Mol. Biol. 2015 Jul; 53(1): 50-9. PMID: 25375039, PMCID: PMC4566110, DOI: 10.1165/rcmb.2014-0289OC, ISSN: 1535-4989.

McKinley ET, Watchmaker JM, Chakravarthy AB, Meyerhardt JA, Engelman JA, Walker RC, Washington MK, Coffey RJ, Manning HC. [(18)F]-FLT PET to predict early response to neoadjuvant therapy in KRAS wild-type rectal cancer: a pilot study. Ann Nucl Med [print-electronic]. 2015 Jul; 29(6): 535-42. PMID: 25899481, PMCID: PMC4503488, DOI: 10.1007/s12149-015-0974-6, ISSN: 1864-6433.

Manning HC. World Molecular Imaging Congress 2015: precision medicine visualized [editorial]. Mol Imaging Biol. 2015 Jun; 17(3): 295-6. PMID: 25862478, DOI: 10.1007/s11307-015-0855-3, ISSN: 1860-2002.

Hassanein M, Hight MR, Buck JR, Tantawy MN, Nickels ML, Hoeksema MD, Harris BK, Boyd K, Massion PP, Manning HC. Preclinical Evaluation of 4-[(18)F]Fluoroglutamine PET to Assess ASCT2 Expression in Lung Cancer. Mol Imaging Biol [print-electronic]. 2015 May 5/14/2015; PMID: 25971659, PMCID: PMC4644503, DOI: 10.1007/s11307-015-0862-4, ISSN: 1860-2002.

Cheung YY, Nickels ML, McKinley ET, Buck JR, Manning HC. High-yielding, automated production of 3'-deoxy-3'-[(18)F]fluorothymidine using a modified Bioscan Coincidence FDG reaction module. Appl Radiat Isot [print-electronic]. 2015 Mar; 97: 47-51. PMID: 25531913, PII: S0969-8043(14)00396-0, DOI: 10.1016/j.apradiso.2014.11.011, ISSN: 1872-9800.

Whisenant JG, McIntyre JO, Peterson TE, Kang H, Sánchez V, Manning HC, Arteaga CL, Yankeelov TE. Utility of [18¿F]FLT-PET to assess treatment response in trastuzumab-resistant and trastuzumab-sensitive HER2-overexpressing human breast cancer xenografts. Mol Imaging Biol. 2015 Feb; 17(1): 119-28. PMID: 25034624, PMCID: PMC4311727, DOI: 10.1007/s11307-014-0770-z, ISSN: 1860-2002.

Buck JR, McKinley ET, Fu A, Abel TW, Thompson RC, Chambless L, Watchmaker JM, Harty JP, Cooper MK, Manning HC. Preclinical TSPO Ligand PET to Visualize Human Glioma Xenotransplants: A Preliminary Study. PLoS ONE. 2015; 10(10): e0141659. PMID: 26517124, PMCID: PMC4627825, PII: PONE-D-15-02293, DOI: 10.1371/journal.pone.0141659, ISSN: 1932-6203.

Schulte ML, Dawson ES, Saleh SA, Cuthbertson ML, Manning HC. 2-Substituted N¿-glutamylanilides as novel probes of ASCT2 with improved potency. Bioorg. Med. Chem. Lett [print-electronic]. 2015 Jan 1/1/2015; 25(1): 113-6. PMID: 25435145, PMCID: PMC4372298, PII: S0960-894X(14)01169-X, DOI: 10.1016/j.bmcl.2014.10.098, ISSN: 1464-3405.

Comerford SA, Huang Z, Du X, Wang Y, Cai L, Witkiewicz AK, Walters H, Tantawy MN, Fu A, Manning HC, Horton JD, Hammer RE, McKnight SL, Tu BP. Acetate dependence of tumors. Cell. 2014 Dec 12/18/2014; 159(7): 1591-602. PMID: 25525877, PMCID: PMC4272450, PII: S0092-8674(14)01451-2, DOI: 10.1016/j.cell.2014.11.020, ISSN: 1097-4172.

Tang D, Nickels ML, Tantawy MN, Buck JR, Manning HC. Preclinical imaging evaluation of novel TSPO-PET ligand 2-(5,7-Diethyl-2-(4-(2-[(18)F]fluoroethoxy)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)-N,N-diethylacetamide ([ (18)F]VUIIS1008) in glioma. Mol Imaging Biol. 2014 Dec; 16(6): 813-20. PMID: 24845529, PMCID: PMC4372299, DOI: 10.1007/s11307-014-0743-2, ISSN: 1860-2002.

Cheung YY, Nickels ML, Tang D, Buck JR, Manning HC. Facile synthesis of SSR180575 and discovery of 7-chloro-N,N,5-trimethyl-4-oxo-3(6-[(18)F]fluoropyridin-2-yl)-3,5-dihydro-4H-pyridazino[4,5-b]indole-1-acetamide, a potent pyridazinoindole ligand for PET imaging of TSPO in cancer. Bioorg. Med. Chem. Lett [print-electronic]. 2014 Sep 9/15/2014; 24(18): 4466-71. PMID: 25172419, PMCID: PMC4163096, PII: S0960-894X(14)00826-9, DOI: 10.1016/j.bmcl.2014.07.091, ISSN: 1464-3405.

Powell AE, Vlacich G, Zhao ZY, McKinley ET, Washington MK, Manning HC, Coffey RJ. Inducible loss of one Apc allele in Lrig1-expressing progenitor cells results in multiple distal colonic tumors with features of familial adenomatous polyposis. Am. J. Physiol. Gastrointest. Liver Physiol [print-electronic]. 2014 Jul 7/1/2014; 307(1): G16-23. PMID: 24833705, PMCID: PMC4080164, PII: ajpgi.00358.2013, DOI: 10.1152/ajpgi.00358.2013, ISSN: 1522-1547.

Perrone MG, Malerba P, Uddin MJ, Vitale P, Panella A, Crews BC, Daniel CK, Ghebreselasie K, Nickels M, Tantawy MN, Manning HC, Marnett LJ, Scilimati A. PET radiotracer [¹8F]-P6 selectively targeting COX-1 as a novel biomarker in ovarian cancer: preliminary investigation. Eur J Med Chem [print-electronic]. 2014 Jun 6/10/2014; 80: 562-8. PMID: 24832612, PMCID: PMC4401082, PII: S0223-5234(14)00403-6, DOI: 10.1016/j.ejmech.2014.04.074, ISSN: 1768-3254.

Hight MR, Cheung YY, Nickels ML, Dawson ES, Zhao P, Saleh S, Buck JR, Tang D, Washington MK, Coffey RJ, Manning HC. A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells. Clin. Cancer Res [print-electronic]. 2014 Apr 4/15/2014; 20(8): 2126-35. PMID: 24573549, PMCID: PMC3989451, PII: 1078-0432.CCR-13-2444, DOI: 10.1158/1078-0432.CCR-13-2444, ISSN: 1078-0432.

Uddin MI, Buck JR, Schulte ML, Tang D, Saleh SA, Cheung YY, Harp J, Manning HC. Microwave-assisted, one-pot reaction of 7-azaindoles and aldehydes: a facile route to novel di-7-azaindolylmethanes. Tetrahedron Lett. 2014 Jan 1/1/2014; 55(1): PMID: 24396154, PMCID: PMC3880151, DOI: 10.1016/j.tetlet.2013.10.143, ISSN: 0040-4039.

McKinley ET, Zhao P, Coffey RJ, Washington MK, Manning HC. 3'-Deoxy-3'-[18F]-Fluorothymidine PET imaging reflects PI3K-mTOR-mediated pro-survival response to targeted therapy in colorectal cancer. PLoS ONE. 2014; 9(9): e108193. PMID: 25247710, PMCID: PMC4172755, PII: PONE-D-14-08668, DOI: 10.1371/journal.pone.0108193, ISSN: 1932-6203.

Katsha A, Soutto M, Sehdev V, Peng D, Washington MK, Piazuelo MB, Tantawy MN, Manning HC, Lu P, Shyr Y, Ecsedy J, Belkhiri A, El-Rifai W. Aurora kinase A promotes inflammation and tumorigenesis in mice and human gastric neoplasia. Gastroenterology [print-electronic]. 2013 Dec; 145(6): 1312-22.e1. PMID: 23993973, PMCID: PMC3840093, PII: S0016-5085(13)01270-5, DOI: 10.1053/j.gastro.2013.08.050, ISSN: 1528-0012.

Walsh AJ, Cook RS, Manning HC, Hicks DJ, Lafontant A, Arteaga CL, Skala MC. Optical metabolic imaging identifies glycolytic levels, subtypes, and early-treatment response in breast cancer. Cancer Res. 2013 Oct 10/15/2013; 73(20): 6164-74. PMID: 24130112, PMCID: PMC3801432, PII: 73/20/6164, DOI: 10.1158/0008-5472.CAN-13-0527, ISSN: 1538-7445.

Xie J, Wang C, Virostko J, Manning HC, Pham W, Bauer J, Gore JC. A novel reporter system for molecular imaging and high-throughput screening of anticancer drugs. Chembiochem [print-electronic]. 2013 Aug 8/19/2013; 14(12): 1494-503. PMID: 23881799, PMCID: PMC3819221, DOI: 10.1002/cbic.201300142, ISSN: 1439-7633.

Tang D, McKinley ET, Hight MR, Uddin MI, Harp JM, Fu A, Nickels ML, Buck JR, Manning HC. Synthesis and structure-activity relationships of 5,6,7-substituted pyrazolopyrimidines: discovery of a novel TSPO PET ligand for cancer imaging. J. Med. Chem [print-electronic]. 2013 Apr 4/25/2013; 56(8): 3429-33. PMID: 23521048, PMCID: PMC3648642, DOI: 10.1021/jm4001874, ISSN: 1520-4804.

McKinley ET, Smith RA, Zhao P, Fu A, Saleh SA, Uddin MI, Washington MK, Coffey RJ, Manning HC. 3'-Deoxy-3'-18F-fluorothymidine PET predicts response to (V600E)BRAF-targeted therapy in preclinical models of colorectal cancer. J. Nucl. Med [print-electronic]. 2013 Mar; 54(3): 424-30. PMID: 23341544, PMCID: PMC3633462, PII: jnumed.112.108456, DOI: 10.2967/jnumed.112.108456, ISSN: 1535-5667.

McKinley ET, Ayers GD, Smith RA, Saleh SA, Zhao P, Washington MK, Coffey RJ, Manning HC. Limits of [18F]-FLT PET as a biomarker of proliferation in oncology. PLoS ONE [print-electronic]. 2013; 8(3): e58938. PMID: 23554961, PMCID: PMC3598948, PII: PONE-D-12-24564, DOI: 10.1371/journal.pone.0058938, ISSN: 1932-6203.

McKinley ET, Liu H, McDonald WH, Luo W, Zhao P, Coffey RJ, Hanks SK, Manning HC. Global phosphotyrosine proteomics identifies PKCd as a marker of responsiveness to Src inhibition in colorectal cancer. PLoS ONE. 2013; 8(11): e80207. PMID: 24260357, PMCID: PMC3832668, PII: PONE-D-13-29839, DOI: 10.1371/journal.pone.0080207, ISSN: 1932-6203.

Thompson MM, Manning HC, Ellacott KL. Translocator protein 18 kDa (TSPO) is regulated in white and brown adipose tissue by obesity. PLoS ONE. 2013; 8(11): e79980. PMID: 24260329, PMCID: PMC3832377, PII: PONE-D-13-18101, DOI: 10.1371/journal.pone.0079980, ISSN: 1932-6203.

McKinley ET, Smith RA, Tanksley JP, Washington MK, Walker R, Coffey RJ, Manning HC. [18F]FLT-PET to predict pharmacodynamic and clinical response to cetuximab therapy in Ménétrier's disease. Ann Nucl Med [print-electronic]. 2012 Nov; 26(9): 757-63. PMID: 22821337, PMCID: PMC3543701, DOI: 10.1007/s12149-012-0636-x, ISSN: 1864-6433.

Yankeelov TE, Peterson TE, Abramson RG, Izquierdo-Garcia D, Arlinghaus LR, Li X, Atuegwu NC, Catana C, Manning HC, Fayad ZA, Gore JC. Simultaneous PET-MRI in oncology: a solution looking for a problem?. Magn Reson Imaging [print-electronic]. 2012 Nov; 30(9): 1342-56. PMID: 22795930, PMCID: PMC3466373, PII: S0730-725X(12)00199-3, DOI: 10.1016/j.mri.2012.06.001, ISSN: 1873-5894.

Buck JR, Saleh S, Uddin MI, Manning HC. Rapid, Microwave-Assisted Organic Synthesis of Selective (V600E)BRAF Inhibitors for Preclinical Cancer Research. Tetrahedron Lett [print-electronic]. 2012 Aug 8/8/2012; 53(32): 4161-5. PMID: 23180892, PMCID: PMC3501741, DOI: 10.1016/j.tetlet.2012.05.137, ISSN: 0040-4039.

Wyatt SK, Manning HC, Bai M, Ehtesham M, Mapara KY, Thompson RC, Bornhop DJ. Preclinical molecular imaging of the translocator protein (TSPO) in a metastases model based on breast cancer xenografts propagated in the murine brain. Curr. Mol. Med. 2012 May; 12(4): 458-66. PMID: 22348613, PII: CMM-EPUB-20120217-006, ISSN: 1875-5666.

Tang D, Hight MR, McKinley ET, Fu A, Buck JR, Smith RA, Tantawy MN, Peterson TE, Colvin DC, Ansari MS, Nickels M, Manning HC. Quantitative preclinical imaging of TSPO expression in glioma using N,N-diethyl-2-(2-(4-(2-18F-fluoroethoxy)phenyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-3-yl)acetamide. J. Nucl. Med [print-electronic]. 2012 Feb; 53(2): 287-94. PMID: 22251555, PMCID: PMC3391587, PII: jnumed.111.095653, DOI: 10.2967/jnumed.111.095653, ISSN: 1535-5667.

Myers MV, Manning HC, Coffey RJ, Liebler DC. Protein expression signatures for inhibition of epidermal growth factor receptor-mediated signaling. Mol. Cell Proteomics [print-electronic]. 2012 Feb; 11(2): M111.015222. PMID: 22147731, PMCID: PMC3277773, PII: M111.015222, DOI: 10.1074/mcp.M111.015222, ISSN: 1535-9484.

Xu J, Li K, Smith RA, Waterton JC, Zhao P, Chen H, Does MD, Manning HC, Gore JC. Characterizing tumor response to chemotherapy at various length scales using temporal diffusion spectroscopy. PLoS ONE [print-electronic]. 2012; 7(7): e41714. PMID: 22911846, PMCID: PMC3404000, PII: PONE-D-12-09797, DOI: 10.1371/journal.pone.0041714, ISSN: 1932-6203.

Fox EM, Miller TW, Balko JM, Kuba MG, Sánchez V, Smith RA, Liu S, González-Angulo AM, Mills GB, Ye F, Shyr Y, Manning HC, Buck E, Arteaga CL. A kinome-wide screen identifies the insulin/IGF-I receptor pathway as a mechanism of escape from hormone dependence in breast cancer. Cancer Res [print-electronic]. 2011 Nov 11/1/2011; 71(21): 6773-84. PMID: 21908557, PMCID: PMC3206206, PII: 0008-5472.CAN-11-1295, DOI: 10.1158/0008-5472.CAN-11-1295, ISSN: 1538-7445.

Miller TW, Balko JM, Fox EM, Ghazoui Z, Dunbier A, Anderson H, Dowsett M, Jiang A, Smith RA, Maira SM, Manning HC, González-Angulo AM, Mills GB, Higham C, Chanthaphaychith S, Kuba MG, Miller WR, Shyr Y, Arteaga CL. ERa-dependent E2F transcription can mediate resistance to estrogen deprivation in human breast cancer. Cancer Discov [print-electronic]. 2011 Sep; 1(4): 338-51. PMID: 22049316, PMCID: PMC3204388, PII: 2159-8290.CD-11-0101, DOI: 10.1158/2159-8290.CD-11-0101, ISSN: 2159-8290.

Gore JC, Manning HC, Quarles CC, Waddell KW, Yankeelov TE. Magnetic resonance in the era of molecular imaging of cancer. Magn Reson Imaging [print-electronic]. 2011 Jun; 29(5): 587-600. PMID: 21524870, PMCID: PMC3285504, PII: S0730-725X(11)00065-8, DOI: 10.1016/j.mri.2011.02.003, ISSN: 1873-5894.

McKinley ET, Bugaj JE, Zhao P, Guleryuz S, Mantis C, Gokhale PC, Wild R, Manning HC. 18FDG-PET predicts pharmacodynamic response to OSI-906, a dual IGF-1R/IR inhibitor, in preclinical mouse models of lung cancer. Clin. Cancer Res [print-electronic]. 2011 May 5/15/2011; 17(10): 3332-40. PMID: 21257723, PMCID: PMC3122480, PII: 1078-0432.CCR-10-2274, DOI: 10.1158/1078-0432.CCR-10-2274, ISSN: 1078-0432.

Guo N, Xie J, Manning HC, Deane NG, Ansari MS, Coffey RJ, Gore J, Price RR, Baldwin RM, McIntyre JO. A novel in vitro assay to assess phosphorylation of 3'-[(18)F]fluoro-3'-deoxythymidine. Mol Imaging Biol. 2011 Apr; 13(2): 257-64. PMID: 20532643, PMCID: PMC4180216, DOI: 10.1007/s11307-010-0351-8, ISSN: 1860-2002.

Garrett JT, Olivares MG, Rinehart C, Granja-Ingram ND, Sánchez V, Chakrabarty A, Dave B, Cook RS, Pao W, McKinely E, Manning HC, Chang J, Arteaga CL. Transcriptional and posttranslational up-regulation of HER3 (ErbB3) compensates for inhibition of the HER2 tyrosine kinase. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2011 Mar 3/22/2011; 108(12): 5021-6. PMID: 21385943, PMCID: PMC3064360, PII: 1016140108, DOI: 10.1073/pnas.1016140108, ISSN: 1091-6490.

Smith RA, Guleryuz S, Manning HC. Molecular imaging metrics to evaluate response to preclinical therapeutic regimens. Front Biosci (Landmark Ed). 2011; 16: 393-410. PMID: 21196177, PMCID: PMC3023459, PII: 3694, ISSN: 1093-4715.

Buck JR, McKinley ET, Hight MR, Fu A, Tang D, Smith RA, Tantawy MN, Peterson TE, Colvin D, Ansari MS, Baldwin RM, Zhao P, Guleryuz S, Manning HC. Quantitative, preclinical PET of translocator protein expression in glioma using 18F-N-fluoroacetyl-N-(2,5-dimethoxybenzyl)-2-phenoxyaniline. J. Nucl. Med [print-electronic]. 2011 Jan; 52(1): 107-14. PMID: 21149488, PMCID: PMC3027353, PII: jnumed.110.081703, DOI: 10.2967/jnumed.110.081703, ISSN: 1535-5667.

Hight MR, Nolting DD, McKinley ET, Lander AD, Wyatt SK, Gonyea M, Zhao P, Manning HC. Multispectral fluorescence imaging to assess pH in biological specimens. J Biomed Opt. 2011 Jan; 16(1): 16007. PMID: 21280913, PMCID: PMC3041815, DOI: 10.1117/1.3533264, ISSN: 1560-2281.

Tang D, Buck JR, Hight MR, Manning HC. Microwave-assisted Organic Synthesis of a High-affinity Pyrazolo-pyrimidinyl TSPO Ligand. Tetrahedron Lett. 2010 Sep 9/1/2010; 51(35): 4595-8. PMID: 20689673, PMCID: PMC2913893, DOI: 10.1016/j.tetlet.2010.06.074, ISSN: 0040-4039.

Wyatt SK, Manning HC, Bai M, Bailey SN, Gallant P, Ma G, McIntosh L, Bornhop DJ. Molecular imaging of the translocator protein (TSPO) in a pre-clinical model of breast cancer. Mol Imaging Biol [print-electronic]. 2010 Jun; 12(3): 349-58. PMID: 19949989, DOI: 10.1007/s11307-009-0270-8, ISSN: 1860-2002.

Ayers GD, McKinley ET, Zhao P, Fritz JM, Metry RE, Deal BC, Adlerz KM, Coffey RJ, Manning HC. Volume of preclinical xenograft tumors is more accurately assessed by ultrasound imaging than manual caliper measurements. J Ultrasound Med. 2010 Jun; 29(6): 891-901. PMID: 20498463, PMCID: PMC2925269, PII: 29/6/891, ISSN: 1550-9613.

Miller TW, Forbes JT, Shah C, Wyatt SK, Manning HC, Olivares MG, Sanchez V, Dugger TC, de Matos Granja N, Narasanna A, Cook RS, Kennedy JP, Lindsley CW, Arteaga CL. Inhibition of mammalian target of rapamycin is required for optimal antitumor effect of HER2 inhibitors against HER2-overexpressing cancer cells. Clin. Cancer Res [print-electronic]. 2009 Dec 12/1/2009; 15(23): 7266-76. PMID: 19934303, PMCID: PMC2787848, PII: 1078-0432.CCR-09-1665, DOI: 10.1158/1078-0432.CCR-09-1665, ISSN: 1078-0432.

Yankeelov TE, Avison MJ, Damon BM, Manning HC, Peterson TE, Gore JC. Frontiers of Biomedical Imaging Science 2009: workshop report and research opportunities. Cancer Res [print-electronic]. 2009 Oct 10/15/2009; 69(20): 7902-4. PMID: 19808955, PMCID: PMC2763022, PII: 0008-5472.CAN-09-2521, DOI: 10.1158/0008-5472.CAN-09-2521, ISSN: 1538-7445.

Peterson TE, Manning HC. Molecular imaging: 18F-FDG PET and a whole lot more. J Nucl Med Technol [print-electronic]. 2009 Sep; 37(3): 151-61. PMID: 19692452, PMCID: PMC2829876, PII: jnmt.109.062729, DOI: 10.2967/jnmt.109.062729, ISSN: 0091-4916.

Shah C, Miller TW, Wyatt SK, McKinley ET, Olivares MG, Sanchez V, Nolting DD, Buck JR, Zhao P, Ansari MS, Baldwin RM, Gore JC, Schiff R, Arteaga CL, Manning HC. Imaging biomarkers predict response to anti-HER2 (ErbB2) therapy in preclinical models of breast cancer. Clin. Cancer Res [print-electronic]. 2009 Jul 7/15/2009; 15(14): 4712-21. PMID: 19584166, PMCID: PMC2819132, PII: 1078-0432.CCR-08-2635, DOI: 10.1158/1078-0432.CCR-08-2635, ISSN: 1078-0432.

Virostko J, Xie J, Hallahan DE, Arteaga CL, Gore JC, Manning HC. A molecular imaging paradigm to rapidly profile response to angiogenesis-directed therapy in small animals. Mol Imaging Biol [print-electronic]. 2009 May; 11(3): 204-12. PMID: 19130143, PMCID: PMC2677126, DOI: 10.1007/s11307-008-0193-9, ISSN: 1860-2002.

Manning HC, Shay SD, Mericle RA. Multispectral molecular imaging of capillary endothelium to facilitate preoperative endovascular brain mapping. J. Neurosurg. 2009 May; 110(5): 975-80. PMID: 19231927, PMCID: PMC2812869, DOI: 10.3171/2008.9.JNS08420, ISSN: 0022-3085.

Sexton M, Woodruff G, Cudaback E, Kreitzer FR, Xu C, Lin YH, Möller T, Bai M, Manning HC, Bornhop D, Stella N. Binding of NIR-conPK and NIR-6T to astrocytomas and microglial cells: evidence for a protein related to TSPO. PLoS ONE. 2009; 4(12): e8271. PMID: 20020060, PMCID: PMC2792720, DOI: 10.1371/journal.pone.0008271, ISSN: 1932-6203.

Manning HC, Merchant NB, Foutch AC, Virostko JM, Wyatt SK, Shah C, McKinley ET, Xie J, Mutic NJ, Washington MK, LaFleur B, Tantawy MN, Peterson TE, Ansari MS, Baldwin RM, Rothenberg ML, Bornhop DJ, Gore JC, Coffey RJ. Molecular imaging of therapeutic response to epidermal growth factor receptor blockade in colorectal cancer. Clin. Cancer Res. 2008 Nov 11/15/2008; 14(22): 7413-22. PMID: 19010858, PMCID: PMC2657180, PII: 14/22/7413, DOI: 10.1158/1078-0432.CCR-08-0239, ISSN: 1078-0432.

Manning HC, Lander A, McKinley E, Mutic NJ. Accelerating the development of novel molecular imaging probes: a role for high-throughput screening. J. Nucl. Med [print-electronic]. 2008 Sep; 49(9): 1401-4. PMID: 18703594, PMCID: PMC2576283, PII: jnumed.108.053009, DOI: 10.2967/jnumed.108.053009, ISSN: 0161-5505.

H. Charles Manning. Optical imaging shines in vivo. Journal of Nuclear Medicine. 2008; 49: 23N-29N.

Deane NG, Manning HC, Foutch AC, Washington MK, Aronow BJ, Bornhop DJ, Coffey RJ. Targeted imaging of colonic tumors in smad3-/- mice discriminates cancer and inflammation. Mol. Cancer Res. 2007 Apr; 5(4): 341-9. PMID: 17426249, PII: 5/4/341, DOI: 10.1158/1541-7786.MCR-06-0225, ISSN: 1541-7786.

Manning HC, Smith SM, Sexton M, Haviland S, Bai M, Cederquist K, Stella N, Bornhop DJ. A peripheral benzodiazepine receptor targeted agent for in vitro imaging and screening. Bioconjug. Chem. 2006 May; 17(3): 735-40. PMID: 16704212, DOI: 10.1021/bc060020b, ISSN: 1043-1802.

Smentek L, Andes Hess B, Cross JP, Charles Manning H, Bornhop DJ. Density-functional theory structures of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid complexes for ions across the lanthanide series. J Chem Phys. 2005 Dec 12/22/2005; 123(24): 244302. PMID: 16396532, DOI: 10.1063/1.2139997, ISSN: 0021-9606.

Manning, HC., Bai, M., Anderson, BM., Lisiak, R., Samuelson, L., Bornhop, D.J. Expeditious synthesis of P-Protected macrocycles in-route to lanthanide chelate metal complexes. Tetrahedron Letters. 2005; 46: 4707-10.

Manning HC, Goebel T, Thompson RC, Price RR, Lee H, Bornhop DJ. Targeted molecular imaging agents for cellular-scale bimodal imaging. Bioconjug. Chem. 2004 Nov; 15(6): 1488-95. PMID: 15546219, DOI: 10.1021/bc049904q, ISSN: 1043-1802.

Manning, HC., Skwierawska, A., Marx, JN., et al. Rapid removal of benzyloxycarbonyl groups from 1,4,7,10-tetraazacyclododecane derivatives by catalytic transfer hydrogenation. Synthetic Communications. 2003; 33((3)): 457-61.

Marcu, L., Vernier, T., Manning, H.C., Salemi, S., Li, A., Craft, C., Gundersen, M., Bornhop, D. Fluorescence microscopy studies of a peripheral-benzodiazepine-receptor-targeted molecular probe for brain tumor imaging. Proceedings of SPIE-The International Society for Optical Engineering. 2003; 5141: 64-9.

Manning HC, Goebel T, Marx JN, Bornhop DJ. Facile, efficient conjugation of a trifunctional lanthanide chelate to a peripheral benzodiazepine receptor ligand. Org. Lett. 2002 Apr 4/4/2002; 4(7): 1075-8. PMID: 11922786, PII: ol017155b, ISSN: 1523-7060.

Griffin, JMM., Skwierawska, AM., Manning, HC., Marx, JN., Bornhop, DJ. Simple, high-yielding synthesis of trifunctional fluorescent lanthanide chelates. Tetrahedron Letters. 2001; 42: 3823-5.

Available Postdoctoral Position Details
Posted: 10/25/2013
Accepting applications for trainees interested in imaging science. Contact H. Charles Manning, Ph.D. for more details. (henry.c.manning@vanderbilt.edu)