Eric Delpire, Ph.D.

Faculty Appointments
Professor of Anesthesiology Professor of Molecular Physiology and Biophysics
Ph.D., Cell Physiology, University of Liege, Liege, BelgiumM.S., Physiology, University of Liege, Liege, BelgiumB.S., Biology, University of Liege, Liege, Belgium
Office Address
Anesthesiology 2215B Garland Ave. MRB IV- Room 1265
Nashville, TN 37232
Research Description
Work in my laboratory focuses on mechanisms that cells and organisms have developed to maintain water and ion homeostasis.
Research Keywords
Cell volume - ion transport - chloride homeostasis - chloride transport - GABA neurotransmission - sodium transport - distal nephron - blood pressure
Waataja JJ, Peterson CD, Verma H, Goracke-Postle CJ, Séguéla P, Delpire E, Wilcox GL, Fairbanks CA. Agmatine preferentially antagonizes GluN2B-containing N-methyl-d-aspartate receptors in spinal cord. J. Neurophysiol [print-electronic]. 2019 Feb 2/1/2019; 121(2): 662-71. PMID: 30427758, DOI: 10.1152/jn.00172.2018, ISSN: 1522-1598.

Flores B, Schornak CC, Delpire E. A role for KCC3 in maintaining cell volume of peripheral nerve fibers. Neurochem. Int [print-electronic]. 2019 Feb; 123: 114-24. PMID: 29366908, PII: S0197-0186(17)30634-4, DOI: 10.1016/j.neuint.2018.01.009, ISSN: 1872-9754.

Delpire E, Gagnon KB. Elusive role of the Na-K-2Cl cotransporter in the choroid plexus. Am. J. Physiol., Cell Physiol [print-electronic]. 2018 Dec 12/21/2018; PMID: 30576234, DOI: 10.1152/ajpcell.00490.2018, ISSN: 1522-1563.

Joffe ME, Turner BD, Delpire E, Grueter BA. Genetic loss of GluN2B in D1-expressing cell types enhances long-term cocaine reward and potentiation of thalamo-accumbens synapses. Neuropsychopharmacology [print-electronic]. 2018 Nov; 43(12): 2383-9. PMID: 29982266, PMCID: PMC6180117, PII: 10.1038/s41386-018-0131-8, DOI: 10.1038/s41386-018-0131-8, ISSN: 1740-634X.

Koumangoye R, Omer S, Delpire E. Mistargeting of a truncated Na-K-2Cl cotransporter in epithelial cells. Am. J. Physiol., Cell Physiol [print-electronic]. 2018 Aug 8/1/2018; 315(2): C258-C276. PMID: 29719172, PMCID: PMC6139505, DOI: 10.1152/ajpcell.00130.2018, ISSN: 1522-1563.

Harris AN, Grimm PR, Lee HW, Delpire E, Fang L, Verlander JW, Welling PA, Weiner ID. Mechanism of Hyperkalemia-Induced Metabolic Acidosis. J. Am. Soc. Nephrol [print-electronic]. 2018 May; 29(5): 1411-25. PMID: 29483157, PMCID: PMC5967781, PII: ASN.2017111163, DOI: 10.1681/ASN.2017111163, ISSN: 1533-3450.

Delpire E, Gagnon KB. Na+ -K+ -2Cl- Cotransporter (NKCC) Physiological Function in Nonpolarized Cells and Transporting Epithelia. Compr Physiol. 2018 Mar 3/25/2018; 8(2): 871-901. PMID: 29687903, DOI: 10.1002/cphy.c170018, ISSN: 2040-4603.

Delpire E, Gagnon KB. Water Homeostasis and Cell Volume Maintenance and Regulation. Curr Top Membr [print-electronic]. 2018; 81: 3-52. PMID: 30243436, PII: S1063-5823(18)30009-7, DOI: 10.1016/bs.ctm.2018.08.001, ISSN: 1063-5823.

Delpire E, Hamilton KL, Hawke TJ, Isenberg JS, Lotersztajn S, Yuan JX, Adams JC. AJP-Cell Physiology begins landmark reviews in cell physiology: an editorial from the senior editors of AJP-Cell Physiology. Am. J. Physiol., Cell Physiol [print-electronic]. 2018 Jan 1/1/2018; 314(1): C1-C2. PMID: 29092820, PMCID: PMC6334934, PII: ajpcell.00256.2017, DOI: 10.1152/ajpcell.00256.2017, ISSN: 1522-1563.

Markkanen M, Ludwig A, Khirug S, Pryazhnikov E, Soni S, Khiroug L, Delpire E, Rivera C, Airaksinen MS, Uvarov P. Implications of the N-terminal heterogeneity for the neuronal K-Cl cotransporter KCC2 function. Brain Res [print-electronic]. 2017 Nov 11/15/2017; 1675: 87-101. PMID: 28888841, PII: S0006-8993(17)30378-5, DOI: 10.1016/j.brainres.2017.08.034, ISSN: 1872-6240.

Koumangoye R, Delpire E. DNPEP is not the only peptidase that produces SPAK fragments in kidney. Physiol Rep. 2017 Nov; 5(21): PMID: 29122955, PMCID: PMC5688775, PII: 5/21/e13479, DOI: 10.14814/phy2.13479, ISSN: 2051-817X.

Bowerman M, Salsac C, Bernard V, Soulard C, Dionne A, Coque E, Benlefki S, Hince P, Dion PA, Butler-Browne G, Camu W, Bouchard JP, Delpire E, Rouleau GA, Raoul C, Scamps F. KCC3 loss-of-function contributes to Andermann syndrome by inducing activity-dependent neuromuscular junction defects. Neurobiol. Dis [print-electronic]. 2017 Oct; 106: 35-48. PMID: 28647557, PII: S0969-9961(17)30140-7, DOI: 10.1016/j.nbd.2017.06.013, ISSN: 1095-953X.

Grimm PR, Coleman R, Delpire E, Welling PA. Constitutively Active SPAK Causes Hyperkalemia by Activating NCC and Remodeling Distal Tubules. J. Am. Soc. Nephrol [print-electronic]. 2017 Sep; 28(9): 2597-606. PMID: 28442491, PMCID: PMC5576927, PII: ASN.2016090948, DOI: 10.1681/ASN.2016090948, ISSN: 1533-3450.

Karimy JK, Zhang J, Kurland DB, Theriault BC, Duran D, Stokum JA, Furey CG, Zhou X, Mansuri MS, Montejo J, Vera A, DiLuna ML, Delpire E, Alper SL, Gunel M, Gerzanich V, Medzhitov R, Simard JM, Kahle KT. Inflammation-dependent cerebrospinal fluid hypersecretion by the choroid plexus epithelium in posthemorrhagic hydrocephalus. Nat. Med [print-electronic]. 2017 Aug; 23(8): 997-1003. PMID: 28692063, PII: nm.4361, DOI: 10.1038/nm.4361, ISSN: 1546-170X.

Zhang J, Karimy JK, Delpire E, Kahle KT. Pharmacological targeting of SPAK kinase in disorders of impaired epithelial transport. Expert Opin. Ther. Targets [print-electronic]. 2017 Aug; 21(8): 795-804. PMID: 28679296, DOI: 10.1080/14728222.2017.1351949, ISSN: 1744-7631.

Glykys J, Dzhala V, Egawa K, Kahle KT, Delpire E, Staley K. Chloride Dysregulation, Seizures, and Cerebral Edema: A Relationship with Therapeutic Potential. Trends Neurosci [print-electronic]. 2017 May; 40(5): 276-94. PMID: 28431741, PMCID: PMC5473173, PII: S0166-2236(17)30046-2, DOI: 10.1016/j.tins.2017.03.006, ISSN: 1878-108X.

Wills TA, Baucum AJ, Holleran KM, Chen Y, Pasek JG, Delpire E, Tabb DL, Colbran RJ, Winder DG. Chronic intermittent alcohol disrupts the GluN2B-associated proteome and specifically regulates group I mGlu receptor-dependent long-term depression. Addict Biol [print-electronic]. 2017 Mar; 22(2): 275-90. PMID: 26549202, PMCID: PMC4860359, DOI: 10.1111/adb.12319, ISSN: 1369-1600.

Shekarabi M, Zhang J, Khanna AR, Ellison DH, Delpire E, Kahle KT. WNK Kinase Signaling in Ion Homeostasis and Human Disease. Cell Metab. 2017 Feb 2/7/2017; 25(2): 285-99. PMID: 28178566, PII: S1550-4131(17)30042-6, DOI: 10.1016/j.cmet.2017.01.007, ISSN: 1932-7420.

Radke AK, Jury NJ, Delpire E, Nakazawa K, Holmes A. Reduced ethanol drinking following selective cortical interneuron deletion of the GluN2B NMDA receptors subunit. Alcohol [print-electronic]. 2017 Feb; 58: 47-51. PMID: 28109345, PII: S0741-8329(15)30154-3, DOI: 10.1016/j.alcohol.2016.07.005, ISSN: 1873-6823.

Delpire E. The mysterious role of the neuronal anion exchanger-3. J. Physiol. (Lond.) [print-electronic]. 2017 Feb 2/1/2017; 595(3): 601-2. PMID: 28097651, PMCID: PMC5285717, DOI: 10.1113/JP273033, ISSN: 1469-7793.

Delpire E, Kahle KT. The KCC3 cotransporter as a therapeutic target for peripheral neuropathy. Expert Opin. Ther. Targets [print-electronic]. 2017 Feb; 21(2): 113-6. PMID: 28019725, DOI: 10.1080/14728222.2017.1275569, ISSN: 1744-7631.

Delpire E, Weaver CD. Challenges of Finding Novel Drugs Targeting the K-Cl Cotransporter. ACS Chem Neurosci [print-electronic]. 2016 Dec 12/21/2016; 7(12): 1624-7. PMID: 27998063, PII: 10.1021/acschemneuro.6b00366, ISSN: 1948-7193.

Kahle KT, Khanna AR, Duan J, Staley KJ, Delpire E, Poduri A. The KCC2 Cotransporter and Human Epilepsy: Getting Excited About Inhibition. Neuroscientist [print-electronic]. 2016 Dec; 22(6): 555-62. PMID: 27130838, PII: 1073858416645087, DOI: 10.1177/1073858416645087, ISSN: 1089-4098.

Delpire E, Wolfe L, Flores B, Koumangoye R, Schornak CC, Omer S, Pusey B, Lau C, Markello T, Adams DR. A patient with multisystem dysfunction carries a truncation mutation in human SLC12A2, the gene encoding the Na-K-2Cl cotransporter, NKCC1. Cold Spring Harb Mol Case Stud. 2016 Nov; 2(6): a001289. PMID: 27900370, PMCID: PMC5111002, PII: DelpireMCS001289, DOI: 10.1101/mcs.a001289.

Sun YT, Tzeng SF, Lin TS, Hsu KS, Delpire E, Shen MR. KCC3 deficiency-induced disruption of paranodal loops and impairment of axonal excitability in the peripheral nervous system. Neuroscience [print-electronic]. 2016 Oct 10/29/2016; 335: 91-102. PMID: 27568057, PII: S0306-4522(16)30404-3, DOI: 10.1016/j.neuroscience.2016.08.031, ISSN: 1873-7544.

Kahle KT, Flores B, Bharucha-Goebel D, Zhang J, Donkervoort S, Hegde M, Hussain G, Duran D, Liang B, Sun D, Bönnemann CG, Delpire E. Peripheral motor neuropathy is associated with defective kinase regulation of the KCC3 cotransporter. Sci Signal. 2016 Aug 8/2/2016; 9(439): ra77. PMID: 27485015, PII: 9/439/ra77, DOI: 10.1126/scisignal.aae0546, ISSN: 1937-9145.

Koumangoye R, Delpire E. The Ste20 kinases SPAK and OSR1 travel between cells through exosomes. Am. J. Physiol., Cell Physiol [print-electronic]. 2016 Jul 7/1/2016; 311(1): C43-53. PMID: 27122160, PMCID: PMC4967142, PII: ajpcell.00080.2016, DOI: 10.1152/ajpcell.00080.2016, ISSN: 1522-1563.

Delpire E. STIM and Orai Proteins in Calcium Signaling: an AJP-Cell Physiology series of Themed Reviews [editorial]. Am. J. Physiol., Cell Physiol. 2016 Mar 3/15/2016; 310(6): C401. PMID: 26979414, PII: 310/6/C401, DOI: 10.1152/ajpcell.C-zh0-7906-editorial.2016, ISSN: 1522-1563.

Lee H, Bach E, Noh J, Delpire E, Kandler K. Hyperpolarization-independent maturation and refinement of GABA/glycinergic connections in the auditory brain stem. J. Neurophysiol [print-electronic]. 2016 Mar; 115(3): 1170-82. PMID: 26655825, PMCID: PMC4808136, PII: jn.00926.2015, DOI: 10.1152/jn.00926.2015, ISSN: 1522-1598.

Kahle KT, Delpire E. Kinase-KCC2 coupling: Cl- rheostasis, disease susceptibility, therapeutic target. J. Neurophysiol [print-electronic]. 2016 Jan 1/1/2016; 115(1): 8-18. PMID: 26510764, PMCID: PMC4760510, PII: jn.00865.2015, DOI: 10.1152/jn.00865.2015, ISSN: 1522-1598.

Delpire E. Research antibodies: do not use them to stain your reputation [editorial]. Am. J. Physiol., Cell Physiol. 2015 Dec 12/1/2015; 309(11): C707-8. PMID: 26628686, PMCID: PMC4725442, PII: 309/11/C707, DOI: 10.1152/ajpcell.zh0-7843-editorial.2015, ISSN: 1522-1563.

Delpire E. Research antibodies: do not use them to stain your reputation [editorial]. Am. J. Physiol., Cell Physiol. 2015 Dec 12/1/2015; 309(11): C707-8. PMID: 26628686, PMCID: PMC4725442, PII: 309/11/C707, DOI: 10.1152/ajpcell.zh0-7843-editorial.2015, ISSN: 1522-1563.

Üner A, Gonçalves GH, Li W, Porceban M, Caron N, Schönke M, Delpire E, Sakimura K, Bjørbæk C. The role of GluN2A and GluN2B NMDA receptor subunits in AgRP and POMC neurons on body weight and glucose homeostasis. Mol Metab. 2015 Oct; 4(10): 678-91. PMID: 26500840, PMCID: PMC4588453, PII: S2212-8778(15)00134-9, DOI: 10.1016/j.molmet.2015.06.010.

Mathieu V, Chantôme A, Lefranc F, Cimmino A, Miklos W, Paulitschke V, Mohr T, Maddau L, Kornienko A, Berger W, Vandier C, Evidente A, Delpire E, Kiss R. Sphaeropsidin A shows promising activity against drug-resistant cancer cells by targeting regulatory volume increase. Cell. Mol. Life Sci [print-electronic]. 2015 Oct; 72(19): 3731-46. PMID: 25868554, PMCID: PMC4943577, PII: 10.1007/s00018-015-1902-6, DOI: 10.1007/s00018-015-1902-6, ISSN: 1420-9071.

Kahle KT, Khanna AR, Alper SL, Adragna NC, Lauf PK, Sun D, Delpire E. K-Cl cotransporters, cell volume homeostasis, and neurological disease. Trends Mol Med [print-electronic]. 2015 Aug; 21(8): 513-23. PMID: 26142773, PMCID: PMC4834970, PII: S1471-4914(15)00117-3, DOI: 10.1016/j.molmed.2015.05.008, ISSN: 1471-499X.

Feng X, Zhang Y, Shao N, Wang Y, Zhuang Z, Wu P, Lee MJ, Liu Y, Wang X, Zhuang J, Delpire E, Gu D, Cai H. Aldosterone modulates thiazide-sensitive sodium chloride cotransporter abundance via DUSP6-mediated ERK1/2 signaling pathway. Am. J. Physiol. Renal Physiol [print-electronic]. 2015 May 5/15/2015; 308(10): F1119-27. PMID: 25761881, PMCID: PMC4437001, PII: ajprenal.00543.2014, DOI: 10.1152/ajprenal.00543.2014, ISSN: 1522-1466.

Wade JB, Liu J, Coleman R, Grimm PR, Delpire E, Welling PA. SPAK-mediated NCC regulation in response to low-K+ diet. Am. J. Physiol. Renal Physiol [print-electronic]. 2015 Apr 4/15/2015; 308(8): F923-31. PMID: 25651563, PMCID: PMC4398835, PII: ajprenal.00388.2014, DOI: 10.1152/ajprenal.00388.2014, ISSN: 1522-1466.

Kamat NV, Thabet SR, Xiao L, Saleh MA, Kirabo A, Madhur MS, Delpire E, Harrison DG, McDonough AA. Renal transporter activation during angiotensin-II hypertension is blunted in interferon-¿-/- and interleukin-17A-/- mice. Hypertension [print-electronic]. 2015 Mar; 65(3): 569-76. PMID: 25601932, PMCID: PMC4326622, PII: HYPERTENSIONAHA.114.04975, DOI: 10.1161/HYPERTENSIONAHA.114.04975, ISSN: 1524-4563.

Vorontsova I, Lam L, Delpire E, Lim J, Donaldson P. Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and human lenses. Invest. Ophthalmol. Vis. Sci. 2014 Dec 12/16/2014; 56(1): 310-21. PMID: 25515571, PMCID: PMC4294287, PII: iovs.14-15911, DOI: 10.1167/iovs.14-15911, ISSN: 1552-5783.

Shisler D, Austin TM, Delpire E, Sawyer DB, Pandey AK. Syndrome of severe pain associated with a continuous bumetanide infusion. Int. J. Cardiol [print-electronic]. 2014 Dec 12/15/2014; 177(2): e61-2. PMID: 25304072, PMCID: PMC4283470, PII: S0167-5273(14)01880-4, DOI: 10.1016/j.ijcard.2014.09.160, ISSN: 1874-1754.

Giani JF, Janjulia T, Kamat N, Seth DM, Blackwell WL, Shah KH, Shen XZ, Fuchs S, Delpire E, Toblli JE, Bernstein KE, McDonough AA, Gonzalez-Villalobos RA. Renal angiotensin-converting enzyme is essential for the hypertension induced by nitric oxide synthesis inhibition. J. Am. Soc. Nephrol [print-electronic]. 2014 Dec; 25(12): 2752-63. PMID: 25012170, PMCID: PMC4243348, PII: ASN.2013091030, DOI: 10.1681/ASN.2013091030, ISSN: 1533-3450.

Ding J, Delpire E. Deletion of KCC3 in parvalbumin neurons leads to locomotor deficit in a conditional mouse model of peripheral neuropathy associated with agenesis of the corpus callosum. Behav. Brain Res [print-electronic]. 2014 Nov 11/1/2014; 274: 128-36. PMID: 25116249, PMCID: PMC4179972, PII: S0166-4328(14)00517-8, DOI: 10.1016/j.bbr.2014.08.005, ISSN: 1872-7549.

Miller OH, Yang L, Wang CC, Hargroder EA, Zhang Y, Delpire E, Hall BJ. GluN2B-containing NMDA receptors regulate depression-like behavior and are critical for the rapid antidepressant actions of ketamine. Elife. 2014 Oct 10/23/2014; 3: e03581. PMID: 25340958, PMCID: PMC4270067, DOI: 10.7554/eLife.03581, ISSN: 2050-084X.

Markadieu N, Rios K, Spiller BW, McDonald WH, Welling PA, Delpire E. Short forms of Ste20-related proline/alanine-rich kinase (SPAK) in the kidney are created by aspartyl aminopeptidase (Dnpep)-mediated proteolytic cleavage. J. Biol. Chem [print-electronic]. 2014 Oct 10/17/2014; 289(42): 29273-84. PMID: 25164821, PMCID: PMC4200278, PII: M114.604009, DOI: 10.1074/jbc.M114.604009, ISSN: 1083-351X.

Delpire E, Staley KJ. Novel determinants of the neuronal Cl(-) concentration. J. Physiol. (Lond.) [print-electronic]. 2014 Oct 10/1/2014; 592(19): 4099-114. PMID: 25107928, PMCID: PMC4215762, PII: jphysiol.2014.275529, DOI: 10.1113/jphysiol.2014.275529, ISSN: 1469-7793.

Terker AS, Yang CL, McCormick JA, Meermeier NP, Rogers SL, Grossmann S, Trompf K, Delpire E, Loffing J, Ellison DH. Sympathetic stimulation of thiazide-sensitive sodium chloride cotransport in the generation of salt-sensitive hypertension. Hypertension [print-electronic]. 2014 Jul; 64(1): 178-84. PMID: 24799612, PMCID: PMC4061471, PII: HYPERTENSIONAHA.114.03335, DOI: 10.1161/HYPERTENSIONAHA.114.03335, ISSN: 1524-4563.

Austin TM, Nannemann DP, Deluca SL, Meiler J, Delpire E. In silico analysis and experimental verification of OSR1 kinase - Peptide interaction. J. Struct. Biol [print-electronic]. 2014 Jul; 187(1): 58-65. PMID: 24821279, PMCID: PMC4082687, PII: S1047-8477(14)00107-5, DOI: 10.1016/j.jsb.2014.05.001, ISSN: 1095-8657.

Mahadevan V, Pressey JC, Acton BA, Uvarov P, Huang MY, Chevrier J, Puchalski A, Li CM, Ivakine EA, Airaksinen MS, Delpire E, McInnes RR, Woodin MA. Kainate receptors coexist in a functional complex with KCC2 and regulate chloride homeostasis in hippocampal neurons. Cell Rep [print-electronic]. 2014 Jun 6/26/2014; 7(6): 1762-70. PMID: 24910435, PII: S2211-1247(14)00397-0, DOI: 10.1016/j.celrep.2014.05.022, ISSN: 2211-1247.

Ponce-Coria J, Markadieu N, Austin TM, Flammang L, Rios K, Welling PA, Delpire E. A novel Ste20-related proline/alanine-rich kinase (SPAK)-independent pathway involving calcium-binding protein 39 (Cab39) and serine threonine kinase with no lysine member 4 (WNK4) in the activation of Na-K-Cl cotransporters. J. Biol. Chem [print-electronic]. 2014 Jun 6/20/2014; 289(25): 17680-8. PMID: 24811174, PMCID: PMC4067202, PII: M113.540518, DOI: 10.1074/jbc.M113.540518, ISSN: 1083-351X.

Markadieu N, Delpire E. Physiology and pathophysiology of SLC12A1/2 transporters. Pflugers Arch [print-electronic]. 2014 Jan; 466(1): 91-105. PMID: 24097229, PMCID: PMC3877717, DOI: 10.1007/s00424-013-1370-5, ISSN: 1432-2013.

McCall NM, Sprow GM, Delpire E, Thiele TE, Kash TL, Pleil KE. Effects of sex and deletion of neuropeptide Y2 receptors from GABAergic neurons on affective and alcohol drinking behaviors in mice. Front Integr Neurosci. 2013; 7: 100. PMID: 24399943, PMCID: PMC3872329, DOI: 10.3389/fnint.2013.00100, ISSN: 1662-5145.

Ding J, Ponce-Coria J, Delpire E. A trafficking-deficient mutant of KCC3 reveals dominant-negative effects on K-Cl cotransport function. PLoS ONE [electronic-print]. 2013; 8(4): e61112. PMID: 23593405, PMCID: PMC3617232, PII: PONE-D-13-06018, DOI: 10.1371/journal.pone.0061112, ISSN: 1932-6203.

Ponce-Coria J, Gagnon KB, Delpire E. Calcium-binding protein 39 facilitates molecular interaction between Ste20p proline alanine-rich kinase and oxidative stress response 1 monomers. Am. J. Physiol., Cell Physiol [print-electronic]. 2012 Dec 12/1/2012; 303(11): C1198-205. PMID: 23034389, PMCID: PMC3530771, PII: ajpcell.00284.2012, DOI: 10.1152/ajpcell.00284.2012, ISSN: 1522-1563.

Gagnon KB, Delpire E. Molecular physiology of SPAK and OSR1: two Ste20-related protein kinases regulating ion transport. Physiol. Rev. 2012 Oct; 92(4): 1577-617. PMID: 23073627, PMCID: PMC4519243, PII: 92/4/1577, DOI: 10.1152/physrev.00009.2012, ISSN: 1522-1210.

Delpire E, Baranczak A, Waterson AG, Kim K, Kett N, Morrison RD, Daniels JS, Weaver CD, Lindsley CW. Further optimization of the K-Cl cotransporter KCC2 antagonist ML077: development of a highly selective and more potent in vitro probe. Bioorg. Med. Chem. Lett [print-electronic]. 2012 Jul 7/15/2012; 22(14): 4532-5. PMID: 22727639, PMCID: PMC3389279, PII: S0960-894X(12)00738-X, DOI: 10.1016/j.bmcl.2012.05.126, ISSN: 1464-3405.

Wills TA, Klug JR, Silberman Y, Baucum AJ, Weitlauf C, Colbran RJ, Delpire E, Winder DG. GluN2B subunit deletion reveals key role in acute and chronic ethanol sensitivity of glutamate synapses in bed nucleus of the stria terminalis. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Jan 1/31/2012; 109(5): E278-87. PMID: 22219357, PMCID: PMC3277158, PII: 1113820109, DOI: 10.1073/pnas.1113820109, ISSN: 1091-6490.

Austin TM, Delpire E. Inhibition of KCC2 in mouse spinal cord neurons leads to hypersensitivity to thermal stimulation. Anesth. Analg [print-electronic]. 2011 Dec; 113(6): 1509-15. PMID: 21965363, PMCID: PMC3224196, PII: ANE.0b013e31822e0a5d, DOI: 10.1213/ANE.0b013e31822e0a5d, ISSN: 1526-7598.

Delpire E, Gagnon KB. Kinetics of hyperosmotically stimulated Na-K-2Cl cotransporter in Xenopus laevis oocytes. Am. J. Physiol., Cell Physiol [print-electronic]. 2011 Nov; 301(5): C1074-85. PMID: 21775703, PMCID: PMC4214853, PII: ajpcell.00131.2011, DOI: 10.1152/ajpcell.00131.2011, ISSN: 1522-1563.

Badanich KA, Doremus-Fitzwater TL, Mulholland PJ, Randall PK, Delpire E, Becker HC. NR2B-deficient mice are more sensitive to the locomotor stimulant and depressant effects of ethanol. Genes Brain Behav [print-electronic]. 2011 Oct; 10(7): 805-16. PMID: 21762461, PMCID: PMC4160738, DOI: 10.1111/j.1601-183X.2011.00720.x, ISSN: 1601-183X.

Gagnon KB, Rios K, Delpire E. Functional insights into the activation mechanism of Ste20-related kinases. Cell. Physiol. Biochem [print-electronic]. 2011; 28(6): 1219-30. PMID: 22179010, PII: 000335854, DOI: 10.1159/000335854, ISSN: 1421-9778.

Delpire E, Gagnon KB, Ledford JJ, Wallace JM. Housing and husbandry of Xenopus laevis affect the quality of oocytes for heterologous expression studies. J. Am. Assoc. Lab. Anim. Sci. 2011 Jan; 50(1): 46-53. PMID: 21333163, PMCID: PMC3035403, ISSN: 1559-6109.

Gagnon KB, Delpire E. Molecular determinants of hyperosmotically activated NKCC1-mediated K+/K+ exchange. J. Physiol. (Lond.) [print-electronic]. 2010 Sep 9/15/2010; 588(Pt 18): 3385-96. PMID: 20530115, PMCID: PMC2988505, PII: jphysiol.2010.191932, DOI: 10.1113/jphysiol.2010.191932, ISSN: 1469-7793.

Delpire E, Austin TM. Kinase regulation of Na+-K+-2Cl- cotransport in primary afferent neurons. J. Physiol. (Lond.) [print-electronic]. 2010 Sep 9/15/2010; 588(Pt 18): 3365-73. PMID: 20498230, PMCID: PMC2988503, PII: jphysiol.2010.190769, DOI: 10.1113/jphysiol.2010.190769, ISSN: 1469-7793.

Gagnon KB, Delpire E. On the substrate recognition and negative regulation of SPAK, a kinase modulating Na+-K+-2Cl- cotransport activity. Am. J. Physiol., Cell Physiol [print-electronic]. 2010 Sep; 299(3): C614-20. PMID: 20463172, PMCID: PMC2944316, PII: ajpcell.00074.2010, DOI: 10.1152/ajpcell.00074.2010, ISSN: 1522-1563.

Gagnon KB, Delpire E. Multiple pathways for protein phosphatase 1 (PP1) regulation of Na-K-2Cl cotransporter (NKCC1) function: the N-terminal tail of the Na-K-2Cl cotransporter serves as a regulatory scaffold for Ste20-related proline/alanine-rich kinase (SPAK) AND PP1. J. Biol. Chem [print-electronic]. 2010 May 5/7/2010; 285(19): 14115-21. PMID: 20223824, PMCID: PMC2863198, PII: M110.112672, DOI: 10.1074/jbc.M110.112672, ISSN: 1083-351X.

Geng Y, Byun N, Delpire E. Behavioral analysis of Ste20 kinase SPAK knockout mice. Behav. Brain Res [print-electronic]. 2010 Apr 4/2/2010; 208(2): 377-82. PMID: 20006650, PMCID: PMC2833419, PII: S0166-4328(09)00731-1, DOI: 10.1016/j.bbr.2009.12.005, ISSN: 1872-7549.

Delpire E. The mammalian family of sterile 20p-like protein kinases. Pflugers Arch [print-electronic]. 2009 Sep; 458(5): 953-67. PMID: 19399514, DOI: 10.1007/s00424-009-0674-y, ISSN: 1432-2013.

Geng Y, Hoke A, Delpire E. The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons. J. Biol. Chem [print-electronic]. 2009 May 5/22/2009; 284(21): 14020-8. PMID: 19307180, PMCID: PMC2682850, PII: M900142200, DOI: 10.1074/jbc.M900142200, ISSN: 0021-9258.

Delpire E, Days E, Lewis LM, Mi D, Kim K, Lindsley CW, Weaver CD. Small-molecule screen identifies inhibitors of the neuronal K-Cl cotransporter KCC2. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2009 Mar 3/31/2009; 106(13): 5383-8. PMID: 19279215, PMCID: PMC2654392, PII: 0812756106, DOI: 10.1073/pnas.0812756106, ISSN: 1091-6490.

Zhu L, Polley N, Mathews GC, Delpire E. NKCC1 and KCC2 prevent hyperexcitability in the mouse hippocampus. Epilepsy Res [print-electronic]. 2008 May; 79(2-3): 201-12. PMID: 18394864, PMCID: PMC2394664, PII: S0920-1211(08)00046-6, DOI: 10.1016/j.eplepsyres.2008.02.005, ISSN: 0920-1211.

Delpire E, Gagnon KB. SPAK and OSR1: STE20 kinases involved in the regulation of ion homoeostasis and volume control in mammalian cells. Biochem. J. 2008 Jan 1/15/2008; 409(2): 321-31. PMID: 18092945, PII: BJ20071324, DOI: 10.1042/BJ20071324, ISSN: 1470-8728.

Garbarini N, Delpire E. The RCC1 domain of protein associated with Myc (PAM) interacts with and regulates KCC2. Cell. Physiol. Biochem [print-electronic]. 2008; 22(1-4): 31-44. PMID: 18769030, PMCID: PMC2535904, PII: 000149781, DOI: 10.1159/000149781, ISSN: 1421-9778.

Byun N, Delpire E. Axonal and periaxonal swelling precede peripheral neurodegeneration in KCC3 knockout mice. Neurobiol. Dis [print-electronic]. 2007 Oct; 28(1): 39-51. PMID: 17659877, PMCID: PMC2242858, PII: S0969-9961(07)00132-5, DOI: 10.1016/j.nbd.2007.06.014, ISSN: 0969-9961.

Gagnon KB, England R, Diehl L, Delpire E. Apoptosis-associated tyrosine kinase scaffolding of protein phosphatase 1 and SPAK reveals a novel pathway for Na-K-2C1 cotransporter regulation. Am. J. Physiol., Cell Physiol [print-electronic]. 2007 May; 292(5): C1809-15. PMID: 17267545, PII: 00580.2006, DOI: 10.1152/ajpcell.00580.2006, ISSN: 0363-6143.

Delpire E, Gagnon KB. Genome-wide analysis of SPAK/OSR1 binding motifs. Physiol. Genomics [print-electronic]. 2007 Jan 1/17/2007; 28(2): 223-31. PMID: 17032814, PII: 00173.2006, DOI: 10.1152/physiolgenomics.00173.2006, ISSN: 1531-2267.

Gagnon KB, England R, Delpire E. A single binding motif is required for SPAK activation of the Na-K-2Cl cotransporter. Cell. Physiol. Biochem. 2007; 20(1-4): 131-42. PMID: 17595523, PII: 000104161, DOI: 10.1159/000104161, ISSN: 1015-8987.

Delpire E, Gagnon KB. SPAK and OSR1, key kinases involved in the regulation of chloride transport. Acta Physiol (Oxf). 2006 May; 187(1-2): 103-13. PMID: 16734747, PII: APS1565, DOI: 10.1111/j.1748-1716.2006.01565.x, ISSN: 1748-1708.

Gagnon KB, England R, Delpire E. Characterization of SPAK and OSR1, regulatory kinases of the Na-K-2Cl cotransporter. Mol. Cell. Biol. 2006 Jan; 26(2): 689-98. PMID: 16382158, PMCID: PMC1346913, PII: 26/2/689, DOI: 10.1128/MCB.26.2.689-698.2006, ISSN: 0270-7306.

Gagnon KB, England R, Delpire E. Volume sensitivity of cation-Cl- cotransporters is modulated by the interaction of two kinases: Ste20-related proline-alanine-rich kinase and WNK4. Am. J. Physiol., Cell Physiol [print-electronic]. 2006 Jan; 290(1): C134-42. PMID: 15930150, PII: 00037.2005, DOI: 10.1152/ajpcell.00037.2005, ISSN: 0363-6143.

Zhu L, Lovinger D, Delpire E. Cortical neurons lacking KCC2 expression show impaired regulation of intracellular chloride. J. Neurophysiol [print-electronic]. 2005 Mar; 93(3): 1557-68. PMID: 15469961, PII: 00616.2004, DOI: 10.1152/jn.00616.2004, ISSN: 0022-3077.

Karadsheh MF, Byun N, Mount DB, Delpire E. Localization of the KCC4 potassium-chloride cotransporter in the nervous system. Neuroscience. 2004; 123(2): 381-91. PMID: 14698746, PII: S0306452203007693, ISSN: 0306-4522.

Delpire E, Piechotta K. STE20 kinases and cation-chloride cotransporters. Adv. Exp. Med. Biol. 2004; 559: 43-53. PMID: 18727226, ISSN: 0065-2598.

Piechotta K, Garbarini N, England R, Delpire E. Characterization of the interaction of the stress kinase SPAK with the Na+-K+-2Cl- cotransporter in the nervous system: evidence for a scaffolding role of the kinase. J. Biol. Chem [print-electronic]. 2003 Dec 12/26/2003; 278(52): 52848-56. PMID: 14563843, PII: M309436200, DOI: 10.1074/jbc.M309436200, ISSN: 0021-9258.

Piechotta K, Lu J, Delpire E. Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). J. Biol. Chem [print-electronic]. 2002 Dec 12/27/2002; 277(52): 50812-9. PMID: 12386165, PII: M208108200, DOI: 10.1074/jbc.M208108200, ISSN: 0021-9258.

Woo NS, Lu J, England R, McClellan R, Dufour S, Mount DB, Deutch AY, Lovinger DM, Delpire E. Hyperexcitability and epilepsy associated with disruption of the mouse neuronal-specific K-Cl cotransporter gene. Hippocampus. 2002; 12(2): 258-68. PMID: 12000122, DOI: 10.1002/hipo.10014, ISSN: 1050-9631.

Delpire E, Mount DB. Human and murine phenotypes associated with defects in cation-chloride cotransport. Annu. Rev. Physiol. 2002; 64: 803-43. PMID: 11826289, PII: 64/1/803, DOI: 10.1146/annurev.physiol.64.081501.155847, ISSN: 0066-4278.

Lauf PK, Zhang J, Delpire E, Fyffe RE, Mount DB, Adragna NC. K-Cl co-transport: immunocytochemical and functional evidence for more than one KCC isoform in high K and low K sheep erythrocytes. Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 2001 Oct; 130(3): 499-509. PMID: 11913461, PII: S1095-6433(01)00421-4, ISSN: 1095-6433.

Karadsheh MF, Delpire E. Neuronal restrictive silencing element is found in the KCC2 gene: molecular basis for KCC2-specific expression in neurons. J. Neurophysiol. 2001 Feb; 85(2): 995-7. PMID: 11160529, ISSN: 0022-3077.

Pearson MM, Lu J, Mount DB, Delpire E. Localization of the K(+)-Cl(-) cotransporter, KCC3, in the central and peripheral nervous systems: expression in the choroid plexus, large neurons and white matter tracts. Neuroscience. 2001; 103(2): 481-91. PMID: 11246162, PII: S0306-4522(00)00567-4, ISSN: 0306-4522.

Lauf PK, Zhang J, Gagnon KB, Delpire E, Fyffe RE, Adragna NC. K-Cl cotransport: immunohistochemical and ion flux studies in human embryonic kidney (HEK293) cells transfected with full-length and C-terminal-domain-truncated KCC1 cDNAs. Cell. Physiol. Biochem. 2001; 11(3): 143-60. PMID: 11410710, PII: 47802, DOI: 47802, ISSN: 1015-8987.

Delpire E. Cation-Chloride Cotransporters in Neuronal Communication. News Physiol. Sci. 2000 Dec; 15: 309-12. PMID: 11390932, ISSN: 0886-1714.

Sung KW, Kirby M, McDonald MP, Lovinger DM, Delpire E. Abnormal GABAA receptor-mediated currents in dorsal root ganglion neurons isolated from Na-K-2Cl cotransporter null mice. J. Neurosci. 2000 Oct 10/15/2000; 20(20): 7531-8. PMID: 11027211, PII: 20/20/7531, ISSN: 1529-2401.

Strange K, Singer TD, Morrison R, Delpire E. Dependence of KCC2 K-Cl cotransporter activity on a conserved carboxy terminus tyrosine residue. Am. J. Physiol., Cell Physiol. 2000 Sep; 279(3): C860-7. PMID: 10942735, ISSN: 0363-6143.

Klingensmith ME, Neville LJ, Delpire E, Wolfe MM, Soybel DI. Gastrin-mediated effects of omeprazole on rat colon mucosa. Surgery. 1999 Aug; 126(2): 272-8. PMID: 10455894, PII: S0039606099001944, ISSN: 0039-6060.

Lu J, Karadsheh M, Delpire E. Developmental regulation of the neuronal-specific isoform of K-Cl cotransporter KCC2 in postnatal rat brains. J. Neurobiol. 1999 Jun 6/15/1999; 39(4): 558-68. PMID: 10380077, PII: 10.1002/(SICI)1097-4695(19990615)39:4<558::AID-NEU9>3.0.CO;2-5, ISSN: 0022-3034.

Mount DB, Mercado A, Song L, Xu J, George AL, Delpire E, Gamba G. Cloning and characterization of KCC3 and KCC4, new members of the cation-chloride cotransporter gene family. J. Biol. Chem. 1999 Jun 6/4/1999; 274(23): 16355-62. PMID: 10347194, ISSN: 0021-9258.

Delpire E, Lu J, England R, Dull C, Thorne T. Deafness and imbalance associated with inactivation of the secretory Na-K-2Cl co-transporter. Nat. Genet. 1999 Jun; 22(2): 192-5. PMID: 10369265, DOI: 10.1038/9713, ISSN: 1061-4036.

Wu Q, Delpire E, Hebert SC, Strange K. Functional demonstration of Na+-K+-2Cl- cotransporter activity in isolated, polarized choroid plexus cells. Am. J. Physiol. 1998 Dec; 275(6 Pt 1): C1565-72. PMID: 9843718, ISSN: 0002-9513.

Mount DB, Delpire E, Gamba G, Hall AE, Poch E, Hoover RS, Hebert SC. The electroneutral cation-chloride cotransporters. J. Exp. Biol. 1998 Jul; 201(Pt 14): 2091-102. PMID: 9639584, ISSN: 0022-0949.

Hallonquist H, Cima RR, Klingensmith ME, Purdy MJ, Delpire E, Zinner MJ, Soybel DI. Selective increase in gastric mucosal mRNA encoding basolateral Na-K-2C1 cotransporter following ileostomy in the rat. J. Gastrointest. Surg. 1998 May; 2(3): 238-43. PMID: 9841980, PII: S1091255X98800188, ISSN: 1091-255X.

Plotkin MD, Snyder EY, Hebert SC, Delpire E. Expression of the Na-K-2Cl cotransporter is developmentally regulated in postnatal rat brains: a possible mechanism underlying GABA's excitatory role in immature brain. J. Neurobiol. 1997 Nov 11/20/1997; 33(6): 781-95. PMID: 9369151, PII: 10.1002/(SICI)1097-4695(19971120)33:6<781::AID-NEU6>3.0.CO;2-5, ISSN: 0022-3034.

Randall J, Thorne T, Delpire E. Partial cloning and characterization of Slc12a2: the gene encoding the secretory Na+-K+-2Cl- cotransporter. Am. J. Physiol. 1997 Oct; 273(4 Pt 1): C1267-77. PMID: 9357771, ISSN: 0002-9513.

Plotkin MD, Kaplan MR, Peterson LN, Gullans SR, Hebert SC, Delpire E. Expression of the Na(+)-K(+)-2Cl- cotransporter BSC2 in the nervous system. Am. J. Physiol. 1997 Jan; 272(1 Pt 1): C173-83. PMID: 9038823, ISSN: 0002-9513.

Delpire E, Kaplan MR, Plotkin MD, Hebert SC. The Na-(K)-Cl cotransporter family in the mammalian kidney: molecular identification and function(s). Nephrol. Dial. Transplant. 1996 Oct; 11(10): 1967-73. PMID: 8918709, ISSN: 0931-0509.

Klingensmith ME, Hallonquist H, McCoy BP, Cima RR, Delpire E, Soybel DI. Pentagastrin selectively modulates levels of mRNAs encoding apical H/K adenosine triphosphatase and basolateral Na-K-Cl cotransporter in rat gastric fundic mucosa. Surgery. 1996 Aug; 120(2): 242-6; discussion 246. PMID: 8751589, PII: S0039-6060(96)80294-7, ISSN: 0039-6060.

Kaplan MR, Plotkin MD, Brown D, Hebert SC, Delpire E. Expression of the mouse Na-K-2Cl cotransporter, mBSC2, in the terminal inner medullary collecting duct, the glomerular and extraglomerular mesangium, and the glomerular afferent arteriole. J. Clin. Invest. 1996 Aug 8/1/1996; 98(3): 723-30. PMID: 8698864, PMCID: PMC507482, DOI: 10.1172/JCI118844, ISSN: 0021-9738.

Kaplan MR, Mount DB, Delpire E. Molecular mechanisms of NaCl cotransport. Annu. Rev. Physiol. 1996; 58: 649-68. PMID: 8815813, DOI: 10.1146/, ISSN: 0066-4278.

Raat NJ, Delpire E, van Os CH, Bindels RJ. Culturing induced expression of basolateral Na+-K+-2Cl- cotransporter BSC2 in proximal tubule, aortic endothelium, and vascular smooth muscle. Pflugers Arch. 1996 Jan; 431(3): 458-60. PMID: 8584442, ISSN: 0031-6768.

Soybel DI, Gullans SR, Maxwell F, Delpire E. Role of basolateral Na(+)-K(+)-Cl- cotransport in HCl secretion by amphibian gastric mucosa. Am. J. Physiol. 1995 Jul; 269(1 Pt 1): C242-9. PMID: 7631751, ISSN: 0002-9513.

Delpire E, Rauchman MI, Beier DR, Hebert SC, Gullans SR. Molecular cloning and chromosome localization of a putative basolateral Na(+)-K(+)-2Cl- cotransporter from mouse inner medullary collecting duct (mIMCD-3) cells. J. Biol. Chem. 1994 Oct 10/14/1994; 269(41): 25677-83. PMID: 7929272, ISSN: 0021-9258.

Wasserman JC, Delpire E, Tonidandel W, Kojima R, Gullans SR. Molecular characterization of ROSIT, a renal osmotic stress-induced Na(+)-Cl(-)-organic solute cotransporter. Am. J. Physiol. 1994 Oct; 267(4 Pt 2): F688-94. PMID: 7943364, ISSN: 0002-9513.

Delpire E, Gullans SR. Cell volume and K+ transport during differentiation of mouse erythroleukemia cells. Am. J. Physiol. 1994 Feb; 266(2 Pt 1): C515-23. PMID: 8141265, ISSN: 0002-9513.

Rauchman MI, Nigam SK, Delpire E, Gullans SR. An osmotically tolerant inner medullary collecting duct cell line from an SV40 transgenic mouse. Am. J. Physiol. 1993 Sep; 265(3 Pt 2): F416-24. PMID: 8214101, ISSN: 0002-9513.

Lauf PK, Bauer J, Adragna NC, Fujise H, Zade-Oppen AM, Ryu KH, Delpire E. Erythrocyte K-Cl cotransport: properties and regulation. Am. J. Physiol. 1992 Nov; 263(5 Pt 1): C917-32. PMID: 1443104, ISSN: 0002-9513.

Delpire E, Lauf PK. Kinetics of DIDS inhibition of swelling-activated K-Cl cotransport in low K sheep erythrocytes. J. Membr. Biol. 1992 Feb; 126(1): 89-96. PMID: 1593613, ISSN: 0022-2631.

Delpire E, Lauf PK. Trans effects of cellular K and Cl on ouabain-resistant Rb(K) influx in low K sheep red blood cells: further evidence for asymmetry of K-Cl cotransport [corrected]. Pflugers Arch. 1991 Nov; 419(5): 540-2. PMID: 1775379, ISSN: 0031-6768.

Delpire E, Lauf PK. Magnesium and ATP dependence of K-Cl co-transport in low K+ sheep red blood cells. J. Physiol. (Lond.). 1991 Sep; 441: 219-31. PMID: 1816372, PMCID: PMC1180194, ISSN: 0022-3751.

Delpire E, Lauf PK. Kinetics of Cl-dependent K fluxes in hyposmotically swollen low K sheep erythrocytes. J. Gen. Physiol. 1991 Feb; 97(2): 173-93. PMID: 2016578, PMCID: PMC2216477, ISSN: 0022-1295.

Delpire E, Cornet M, Gilles R. Volume regulation in rat pheochromocytoma cultured cells submitted to hypoosmotic conditions. Arch Int Physiol Biochim Biophys. 1991 Feb; 99(1): 71-6. PMID: 1713489, ISSN: 0778-3124.

Buche A, Ouassaidi A, Hacha R, Delpire E, Gilles R, Houssier C. Glycine and other amino compounds prevent chromatin precipitation at physiological ionic strength. FEBS Lett. 1989 Apr 4/24/1989; 247(2): 367-70. PMID: 2714440, PII: 0014-5793(89)81372-9, ISSN: 0014-5793.

Delpire E, Duchêne C, Cornet M, Gilles R. Amiloride: an inhibitor of regulatory volume decrease in rat pheochromocytoma cultured cells. Pflugers Arch. 1988 Feb; 411(2): 223-5. PMID: 3357762, ISSN: 0031-6768.

Cornet M, Delpire E, Gilles R. Relations between cell volume control, microfilaments and microtubules networks in T2 and PC12 cultured cells. J. Physiol. (Paris). 1988; 83(1): 43-9. PMID: 3183978, ISSN: 0021-7948.

Cornet M, Delpire E, Gilles R. Study of microfilaments network during volume regulation process of cultured PC 12 cells. Pflugers Arch. 1987 Sep; 410(1-2): 223-5. PMID: 3684507, ISSN: 0031-6768.

Delpire E, Duchêne C, Goessens G, Gilles R. Effects of osmotic shocks on the ultrastructure of different tissues and cell types. Exp. Cell Res. 1985 Sep; 160(1): 106-16. PMID: 4043240, PII: 0014-4827(85)90240-X, ISSN: 0014-4827.