Julie A. Bastarache, M.D.

Associate Professor

Faculty Appointments
Associate Professor of Medicine Associate Professor of Cell & Developmental BiologyAssociate Professor of Pathology, Microbiology and Immunology
M.D., Medicine, Vanderbilt University, Nashville, TennesseeB.A., Biology, College of the Holy Cross, Worcester, Massachusetts
Office Address
T-1217 MCN
1161 21st Avenue South
Nashville, TN 37232
Research Description
The goal of my research program is to understand the pathophysiology of acute respiratory distress syndrome (ARDS) and sepsis and to uncover novel approaches to prevention and treatment of these devastating conditions. As a physician-scientist, my aim is to uncover novel mechanisms that contribute to the development of sepsis and ARDS with the ultimate goal of identifying new biologic targets and unique therapeutic approaches that can be translated to clinical practice.
My lab has two major focus areas: the role of cell free-hemoglobin in modulating endothelial and lung epithelial permeability in sepsis and ARDS and the role of tissue factor, the initiator of the extrinsic coagulation cascade, in mediating lung epithelial injury and repair. I have experience with mouse models of acute lung injury as well as with modeling the epithelial barrier for in vitro study. I have developed several novel murine and cell culture model systems to study the function of TF in ALI. My research is translational and combines studies in cell culture, human and mouse experimental models, and human samples from patients with sepsis and ARDS.
Research Keywords
Translational research in acute lung injury and sepsis focusing on molecular mechanisms, tissue factor. coagulation cascade, cell-free hemoglobin and gender.
Rondina MT, Tatsumi K, Bastarache JA, Mackman N. Microvesicle Tissue Factor Activity and Interleukin-8 Levels are Associated with Mortality in Patients with Influenza A/H1N1 Infection. Crit. Care Med. 2016 Jul; 44(7): e574-8. PMID: 26751613, PMCID: PMC4911267, DOI: 10.1097/CCM.0000000000001584, ISSN: 1530-0293.

Antoniak S, Tatsumi K, Hisada Y, Milner JJ, Neidich SD, Shaver CM, Pawlinski R, Beck MA, Bastarache JA, Mackman N. Tissue factor deficiency increases alveolar hemorrhage and death in influenza A virus-infected mice. J. Thromb. Haemost [print-electronic]. 2016 Jun; 14(6): 1238-48. PMID: 26947929, DOI: 10.1111/jth.13307, ISSN: 1538-7836.

Bastarache JA, Seeley EJ. Sepsis [editorial]. Clin. Chest Med. 2016 Jun; 37(2): xv-xvi. PMID: 27229652, PII: S0272-5231(16)30025-9, DOI: 10.1016/j.ccm.2016.04.001, ISSN: 1557-8216.

Shaver CM, Upchurch CP, Janz DR, Grove BS, Putz ND, Wickersham NE, Dikalov SI, Ware LB, Bastarache JA. Cell-free hemoglobin: a novel mediator of acute lung injury. Am. J. Physiol. Lung Cell Mol. Physiol [print-electronic]. 2016 Mar 3/15/2016; 310(6): L532-41. PMID: 26773065, PMCID: PMC4796260, PII: ajplung.00155.2015, DOI: 10.1152/ajplung.00155.2015, ISSN: 1522-1504.

Shaver CM, Grove BS, Clune JK, Mackman N, Ware LB, Bastarache JA. Myeloid tissue factor does not modulate lung inflammation or permeability during experimental acute lung injury. Sci Rep. 2016; 6: 22249. PMID: 26924425, PMCID: PMC4770302, PII: srep22249, DOI: 10.1038/srep22249, ISSN: 2045-2322.

Shaver CM, Grove BS, Putz ND, Clune JK, Lawson WE, Carnahan RH, Mackman N, Ware LB, Bastarache JA. Regulation of alveolar procoagulant activity and permeability in direct acute lung injury by lung epithelial tissue factor. Am. J. Respir. Cell Mol. Biol. 2015 Nov; 53(5): 719-27. PMID: 25884207, PMCID: PMC4742948, DOI: 10.1165/rcmb.2014-0179OC, ISSN: 1535-4989.

Shaver CM, Chen W, Janz DR, May AK, Darbar D, Bernard GR, Bastarache JA, Ware LB. Atrial Fibrillation Is an Independent Predictor of Mortality in Critically Ill Patients. Crit. Care Med. 2015 Oct; 43(10): 2104-11. PMID: 26154932, PMCID: PMC4725582, DOI: 10.1097/CCM.0000000000001166, ISSN: 1530-0293.

Chen W, Janz DR, Bastarache JA, May AK, O'Neal HR, Bernard GR, Ware LB. Prehospital aspirin use is associated with reduced risk of acute respiratory distress syndrome in critically ill patients: a propensity-adjusted analysis. Crit. Care Med. 2015 Apr; 43(4): 801-7. PMID: 25559436, PMCID: PMC4359645, DOI: 10.1097/CCM.0000000000000789, ISSN: 1530-0293.

Janz DR, Bastarache JA, Rice TW, Bernard GR, Warren MA, Wickersham N, Sills G, Oates JA, Roberts LJ, Ware LB, . Randomized, placebo-controlled trial of acetaminophen for the reduction of oxidative injury in severe sepsis: the Acetaminophen for the Reduction of Oxidative Injury in Severe Sepsis trial. Crit. Care Med. 2015 Mar; 43(3): 534-41. PMID: 25474535, PMCID: PMC4336619, DOI: 10.1097/CCM.0000000000000718, ISSN: 1530-0293.

Bastarache JA, Wynn JL, Ware LB. Fanning the Fire: Can Methemoglobin Enhance Neutrophil Activation?. EBioMedicine. 2015 Mar; 2(3): 184-5. PMID: 26137557, PMCID: PMC4485487, PII: S2352-3964(15)00043-2, DOI: 10.1016/j.ebiom.2015.01.019, ISSN: 2352-3964.

Ware LB, Bastarache JA, Calfee CS. ARDS: new mechanistic insights, new therapeutic directions [editorial]. Clin. Chest Med [print-electronic]. 2014 Dec; 35(4): xv-xvi. PMID: 25453428, PII: S0272-5231(14)00086-0, DOI: 10.1016/j.ccm.2014.09.001, ISSN: 1557-8216.

Shaver CM, Bastarache JA. Clinical and biological heterogeneity in acute respiratory distress syndrome: direct versus indirect lung injury. Clin. Chest Med [print-electronic]. 2014 Dec; 35(4): 639-53. PMID: 25453415, PMCID: PMC4254695, PII: S0272-5231(14)00073-2, DOI: 10.1016/j.ccm.2014.08.004, ISSN: 1557-8216.

Bastarache JA, Koyama T, Wickersham NE, Ware LB. Validation of a multiplex electrochemiluminescent immunoassay platform in human and mouse samples. J. Immunol. Methods [print-electronic]. 2014 Jun; 408: 13-23. PMID: 24768796, PMCID: PMC4120713, PII: S0022-1759(14)00123-9, DOI: 10.1016/j.jim.2014.04.006, ISSN: 1872-7905.

Bastarache JA, Roberts LJ, Ware LB. Thinking outside the cell: how cell-free hemoglobin can potentiate acute lung injury [editorial]. Am. J. Physiol. Lung Cell Mol. Physiol [print-electronic]. 2014 Feb; 306(3): L231-2. PMID: 24337924, PMCID: PMC3920200, PII: ajplung.00355.2013, DOI: 10.1152/ajplung.00355.2013, ISSN: 1522-1504.

Richmond BW, Worrell JA, Bastarache JA, Gervich DH, Slattery WR, Loyd JE. Histoplasmomas of uncommon size. Chest. 2013 Jun; 143(6): 1795-8. PMID: 23732591, PII: S0012-3692(13)60414-6, DOI: 10.1378/chest.12-2071, ISSN: 1931-3543.

Sebag SC, Bastarache JA, Ware LB. Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells. J. Biol. Chem [print-electronic]. 2013 Mar 3/15/2013; 288(11): 7875-84. PMID: 23362270, PMCID: PMC3597825, PII: M112.403220, DOI: 10.1074/jbc.M112.403220, ISSN: 1083-351X.

Janz DR, Bastarache JA, Peterson JF, Sills G, Wickersham N, May AK, Roberts LJ, Ware LB. Association between cell-free hemoglobin, acetaminophen, and mortality in patients with sepsis: an observational study. Crit. Care Med. 2013 Mar; 41(3): 784-90. PMID: 23314583, PMCID: PMC3578977, DOI: 10.1097/CCM.0b013e3182741a54, ISSN: 1530-0293.

Janz DR, Zhao Z, Koyama T, May AK, Bernard GR, Bastarache JA, Young PP, Ware LB. Longer storage duration of red blood cells is associated with an increased risk of acute lung injury in patients with sepsis. Ann Intensive Care. 2013; 3(1): 33. PMID: 24059842, PMCID: PMC3848804, PII: 2110-5820-3-33, DOI: 10.1186/2110-5820-3-33, ISSN: 2110-5820.

Bastarache JA, Sebag SC, Clune JK, Grove BS, Lawson WE, Janz DR, Roberts LJ, Dworski R, Mackman N, Ware LB. Low levels of tissue factor lead to alveolar haemorrhage, potentiating murine acute lung injury and oxidative stress. Thorax [print-electronic]. 2012 Dec; 67(12): 1032-9. PMID: 23033361, PMCID: PMC3652432, PII: thoraxjnl-2012-201781, DOI: 10.1136/thoraxjnl-2012-201781, ISSN: 1468-3296.

Bastarache JA, Ware LB, Girard TD, Wheeler AP, Rice TW. Markers of inflammation and coagulation may be modulated by enteral feeding strategy. JPEN J Parenter Enteral Nutr [print-electronic]. 2012 Nov; 36(6): 732-40. PMID: 22318965, PMCID: PMC3726194, PII: 0148607111433054, DOI: 10.1177/0148607111433054, ISSN: 0148-6071.

Zeyed YF, Bastarache JA, Matthay MA, Ware LB. The severity of shock is associated with impaired rates of net alveolar fluid clearance in clinical acute lung injury. Am. J. Physiol. Lung Cell Mol. Physiol [print-electronic]. 2012 Sep 9/15/2012; 303(6): L550-5. PMID: 22821995, PMCID: PMC3468480, PII: ajplung.00190.2012, DOI: 10.1152/ajplung.00190.2012, ISSN: 1522-1504.

Bastarache JA, Diamond JM, Kawut SM, Lederer DJ, Ware LB, Christie JD. Postoperative estradiol levels associate with development of primary graft dysfunction in lung transplantation patients. Gend Med [print-electronic]. 2012 Jun; 9(3): 154-65. PMID: 22361838, PMCID: PMC3374892, PII: S1550-8579(12)00049-6, DOI: 10.1016/j.genm.2012.01.009, ISSN: 1878-7398.

Bastarache JA, Sebag SC, Grove BS, Ware LB. Interferon-¿ and tumor necrosis factor-a act synergistically to up-regulate tissue factor in alveolar epithelial cells. Exp. Lung Res. 2011 Oct; 37(8): 509-17. PMID: 21913843, PMCID: PMC3652424, DOI: 10.3109/01902148.2011.605512, ISSN: 1521-0499.

Sebag SC, Bastarache JA, Ware LB. Therapeutic modulation of coagulation and fibrinolysis in acute lung injury and the acute respiratory distress syndrome. Curr Pharm Biotechnol. 2011 Sep; 12(9): 1481-96. PMID: 21401517, PMCID: PMC3893117, PII: BSP/CPB/E-Pub/000104-12-9, ISSN: 1873-4316.

Bastarache JA, Ong T, Matthay MA, Ware LB. Alveolar fluid clearance is faster in women with acute lung injury compared to men. J Crit Care [print-electronic]. 2011 Jun; 26(3): 249-56. PMID: 20688464, PMCID: PMC2982881, PII: S0883-9441(10)00168-1, DOI: 10.1016/j.jcrc.2010.06.005, ISSN: 1557-8615.

Bastarache JA, Koyama T, Wickersham NE, Mitchell DB, Mernaugh RL, Ware LB. Accuracy and reproducibility of a multiplex immunoassay platform: a validation study. J. Immunol. Methods [print-electronic]. 2011 Mar 3/31/2011; 367(1-2): 33-9. PMID: 21277854, PMCID: PMC3108329, PII: S0022-1759(11)00008-1, DOI: 10.1016/j.jim.2011.01.005, ISSN: 1872-7905.

Ware LB, Fremont RD, Bastarache JA, Calfee CS, Matthay MA. Determining the aetiology of pulmonary oedema by the oedema fluid-to-plasma protein ratio. Eur. Respir. J [print-electronic]. 2010 Feb; 35(2): 331-7. PMID: 19741024, PMCID: PMC2819058, PII: 09031936.00098709, DOI: 10.1183/09031936.00098709, ISSN: 1399-3003.

Bastarache JA, Fremont RD, Kropski JA, Bossert FR, Ware LB. Procoagulant alveolar microparticles in the lungs of patients with acute respiratory distress syndrome. Am. J. Physiol. Lung Cell Mol. Physiol [print-electronic]. 2009 Dec; 297(6): L1035-41. PMID: 19700643, PMCID: PMC2793184, PII: 00214.2009, DOI: 10.1152/ajplung.00214.2009, ISSN: 1522-1504.

Bastarache JA, Blackwell TS. Development of animal models for the acute respiratory distress syndrome. Dis Model Mech. 2009 May; 2(5-6): 218-23. PMID: 19407329, PMCID: PMC2675821, PII: 2/5-6/218, DOI: 10.1242/dmm.001677, ISSN: 1754-8411.

Bastarache JA, Wang L, Wang Z, Albertine KH, Matthay MA, Ware LB. Intra-alveolar tissue factor pathway inhibitor is not sufficient to block tissue factor procoagulant activity. Am. J. Physiol. Lung Cell Mol. Physiol [print-electronic]. 2008 May; 294(5): L874-81. PMID: 18310227, PMCID: PMC2764534, PII: 00372.2007, DOI: 10.1152/ajplung.00372.2007, ISSN: 1040-0605.

Wang L, Bastarache JA, Ware LB. The coagulation cascade in sepsis. Curr. Pharm. Des. 2008; 14(19): 1860-9. PMID: 18691097, ISSN: 1873-4286.

Worrell JA, Donnelly EF, Martin JB, Bastarache JA, Loyd JE. Computed tomography and the idiopathic form of proliferative fibrosing mediastinitis. J Thorac Imaging. 2007 Aug; 22(3): 235-40. PMID: 17721332, PII: 00005382-200708000-00004, DOI: 10.1097/01.rti.0000213589.29472.18, ISSN: 0883-5993.

Bastarache JA, Wang L, Geiser T, Wang Z, Albertine KH, Matthay MA, Ware LB. The alveolar epithelium can initiate the extrinsic coagulation cascade through expression of tissue factor. Thorax [print-electronic]. 2007 Jul; 62(7): 608-16. PMID: 17356058, PMCID: PMC2117249, PII: thx.2006.063305, DOI: 10.1136/thx.2006.063305, ISSN: 0040-6376.

Wang L, Bastarache JA, Wickersham N, Fang X, Matthay MA, Ware LB. Novel role of the human alveolar epithelium in regulating intra-alveolar coagulation. Am. J. Respir. Cell Mol. Biol [print-electronic]. 2007 Apr; 36(4): 497-503. PMID: 17099142, PMCID: PMC1899324, PII: 2005-0425OC, DOI: 10.1165/rcmb.2005-0425OC, ISSN: 1044-1549.

Bastarache JA, Ware LB, Bernard GR. The role of the coagulation cascade in the continuum of sepsis and acute lung injury and acute respiratory distress syndrome. Semin Respir Crit Care Med. 2006 Aug; 27(4): 365-76. PMID: 16909370, DOI: 10.1055/s-2006-948290, ISSN: 1069-3424.

Ware LB, Bastarache JA, Wang L. Coagulation and fibrinolysis in human acute lung injury--new therapeutic targets?. Keio J Med. 2005 Sep; 54(3): 142-9. PMID: 16237276, ISSN: 0022-9717.

Available Postdoctoral Position Details
Posted: 8/14/2017

Description of Position

A postdoctoral position is immediately available in the laboratories of Dr. Lorraine Ware, Professor of Medicine and Pathology, Microbioloby and Immunology and Dr. Julie Bastarache, Department of Medicine at Vanderbilt University School of Medicine. Our translational research group studies pathogenic mechanisms of sepsis and acute lung injury with a specific focus on mechanisms of lung epithelial and endothelial injury. A major focus is on understanding the cellular and molecular mechanisms of cell-free hemoglobin-mediated lung epithelial and microvascular endothelial permeability. This postdoctoral fellowship will afford the fellow the opportunity to drive this project forward using a multitude of tools and models available in our group including primary and immortalized cell culture, multiple genetically modifed mouse lines, mouse models of acute lung injury and sepsis as well as the ex vivo perfused human lung.

Qualifications for the Position

Candidates must have an MD and/or PhD in a related medical or biological sciences field. Successful candidates should be self-starters and highly motivated to thrive in a rich research environment. Expertise with cell culture and mouse studies is required. Specific expertise in mitochondrial biology, apoptosis, and/or redox biology would be helpful but is not required. The successful candidate should have a demonstrated ability to work both independently and collaboratively, possess strong oral and written communication skills and have a strong foundation in bench science. Responsibilities include designing and implementing experiments, data collection and review, abstract and manuscript preparation and local and national scientific presentations. A successful fellow will leave the program with a broad knowledge of acute lung injury and sepsis, a robust scientific skillset, expertise in a wide range of laboratory techniques and an opportunity for presentation and publication. Questions about the position can be directed to Lorraine.ware@vanderbilt.edu or Julie.bastarache@vanderbilt.edu.