Kevin G. Osteen, Ph.D.

Professor

kevin.osteen@vanderbilt.edu
Faculty Appointments
Professor of Obstetrics and Gynecology Adjunct Professor of Obstetrics and Gynecology at Meharry Medical CollegePierre Soupart Chair in Obstetrics and GynecologyProfessor of Pathology, Microbiology and Immunology
Education
Ph.D., Endocrinology, Medical College of Georgia, Augusta, GeorgiaB.S., Biology, University of South Carolina, Spartanburg, South Carolina
Office Address
B-1100 MCN
TN 2519
Research Description
Among normal adult tissues, the endometrial lining of the human uterus undergoes perhaps the most dynamic cycle of steroid controlled growth, differentiation and tissue breakdown. The rate of estrogen-driven cell growth during the proliferative phase of the menstrual cycle rivals that of many tumors while progesterone inhibits endometrial growth and appears to provide protection from the development of both benign diseases and certain neoplasms. Ovarian steroids clearly control the endometrium and endometrial activity; however, steroids alone cannot account for the entire orchestration of such well-controlled and often focal patterns of cellular proliferation and differentiation which are observed. To better understand steroid sensitive tissue behavior, my research laboratory has focused on the endometrium as a model to identify interrelationships between steroid action and stromal-epithelial cell communication via specific paracrine factors. Of special interest to us are the cellular and molecular mechanisms which control steroid-dependent versus steroid-independent expressions of metalloproteinases. These enzymes are necessary for degradation of extracellular matrix proteins and play important roles in the extensive tissue remodeling which normally occurs during the menstrual cycle as well as during establishment of the hemochorial placenta. Metalloproteinases may also contribute to the pathophysiology of endometrial dysfunction including abnormal bleeding, defects of placentation and the extrauterine disease endometriosis. Recently, we have extended our studies to investigate altered metalloproteinase gene and protein expression relative to the reproductive toxicology of dioxin.
Research Keywords
reproductive medicine, human endometium, endometrial dysfunction, endometriosis, progesterone action, environmental endocrine disruptors, developmental exposure
Publications
Halder SK, Osteen KG, Al-Hendy A. 1,25-dihydroxyvitamin d3 reduces extracellular matrix-associated protein expression in human uterine fibroid cells. Biol. Reprod [electronic-print]. 2013 Dec; 89(6): 150. PMID: 24174578, PMCID: PMC4076359, PII: biolreprod.113.107714, DOI: 10.1095/biolreprod.113.107714, ISSN: 1529-7268.

Halder SK, Osteen KG, Al-Hendy A. Vitamin D3 inhibits expression and activities of matrix metalloproteinase-2 and -9 in human uterine fibroid cells. Hum. Reprod [print-electronic]. 2013 Sep; 28(9): 2407-16. PMID: 23814095, PMCID: PMC3748859, PII: det265, DOI: 10.1093/humrep/det265, ISSN: 1460-2350.

Bruner-Tran KL, Herington JL, Duleba AJ, Taylor HS, Osteen KG. Medical management of endometriosis: emerging evidence linking inflammation to disease pathophysiology. Minerva Ginecol. 2013 Apr; 65(2): 199-213. PMID: 23598784, PMCID: PMC3718308, PII: R09133452, ISSN: 0026-4784.

Villanueva JA, Sokalska A, Cress AB, Ortega I, Bruner-Tran KL, Osteen KG, Duleba AJ. Resveratrol potentiates effect of simvastatin on inhibition of mevalonate pathway in human endometrial stromal cells. J. Clin. Endocrinol. Metab [print-electronic]. 2013 Mar; 98(3): E455-62. PMID: 23386644, PMCID: PMC3590465, PII: jc.2012-3387, DOI: 10.1210/jc.2012-3387, ISSN: 1945-7197.

Sokalska A, Anderson M, Villanueva J, Ortega I, Bruner-Tran KL, Osteen KG, Duleba AJ. Effects of simvastatin on retinoic acid system in primary human endometrial stromal cells and in a chimeric model of human endometriosis. J. Clin. Endocrinol. Metab [print-electronic]. 2013 Mar; 98(3): E463-71. PMID: 23337719, PMCID: PMC3590479, PII: jc.2012-3402, DOI: 10.1210/jc.2012-3402, ISSN: 1945-7197.

Herington JL, Glore DR, Lucas JA, Osteen KG, Bruner-Tran KL. Dietary fish oil supplementation inhibits formation of endometriosis-associated adhesions in a chimeric mouse model. Fertil. Steril [print-electronic]. 2013 Feb; 99(2): 543-50. PMID: 23103017, PMCID: PMC3582352, PII: S0015-0282(12)02307-2, DOI: 10.1016/j.fertnstert.2012.10.007, ISSN: 1556-5653.

Resuehr D, Glore DR, Taylor HS, Bruner-Tran KL, Osteen KG. Progesterone-dependent regulation of endometrial cannabinoid receptor type 1 (CB1-R) expression is disrupted in women with endometriosis and in isolated stromal cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Fertil. Steril [print-electronic]. 2012 Oct; 98(4): 948-56.e1. PMID: 22789143, PMCID: PMC3462249, PII: S0015-0282(12)00644-9, DOI: 10.1016/j.fertnstert.2012.06.009, ISSN: 1556-5653.

Bruner-Tran KL, Resuehr D, Ding T, Lucas JA, Osteen KG. The Role of Endocrine Disruptors in the Epigenetics of Reproductive Disease and Dysfunction: Potential Relevance to Humans. Curr Obstet Gynecol Rep. 2012 Sep 9/1/2012; 1(3): 116-23. PMID: 24932427, PMCID: PMC4056573, DOI: 10.1007/s13669-012-0014-7, ISSN: 2161-3303.

Sokalska A, Cress A, Bruner-Tran KL, Osteen KG, Taylor HS, Ortega I, Duleba AJ. Simvastatin decreases invasiveness of human endometrial stromal cells. Biol. Reprod [electronic-print]. 2012 Jul; 87(1): 2, 1-6. PMID: 22492974, PMCID: PMC3406552, PII: biolreprod.111.098806, DOI: 10.1095/biolreprod.111.098806, ISSN: 1529-7268.

Cakmak H, Basar M, Seval-Celik Y, Osteen KG, Duleba AJ, Taylor HS, Lockwood CJ, Arici A. Statins inhibit monocyte chemotactic protein 1 expression in endometriosis. Reprod Sci [print-electronic]. 2012 Jun; 19(6): 572-9. PMID: 22267540, PMCID: PMC3439122, PII: 1933719111430998, DOI: 10.1177/1933719111430998, ISSN: 1933-7205.

Taylor HS, Osteen KG, Bruner-Tran KL, Lockwood CJ, Krikun G, Sokalska A, Duleba AJ. Novel therapies targeting endometriosis. Reprod Sci [print-electronic]. 2011 Sep; 18(9): 814-23. PMID: 21693775, PMCID: PMC4046304, PII: 1933719111410713, DOI: 10.1177/1933719111410713, ISSN: 1933-7205.

Herington JL, Bruner-Tran KL, Lucas JA, Osteen KG. Immune interactions in endometriosis. Expert Rev Clin Immunol. 2011 Sep; 7(5): 611-26. PMID: 21895474, PMCID: PMC3204940, DOI: 10.1586/eci.11.53, ISSN: 1744-8409.

McConaha ME, Ding T, Lucas JA, Arosh JA, Osteen KG, Bruner-Tran KL. Preconception omega-3 fatty acid supplementation of adult male mice with a history of developmental 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure prevents preterm birth in unexposed female partners. Reproduction [print-electronic]. 2011 Aug; 142(2): 235-41. PMID: 21653731, PMCID: PMC3730265, PII: REP-11-0070, DOI: 10.1530/REP-11-0070, ISSN: 1741-7899.

Ding T, McConaha M, Boyd KL, Osteen KG, Bruner-Tran KL. Developmental dioxin exposure of either parent is associated with an increased risk of preterm birth in adult mice. Reprod. Toxicol [print-electronic]. 2011 Apr; 31(3): 351-8. PMID: 21093581, PMCID: PMC3075349, PII: S0890-6238(10)00327-8, DOI: 10.1016/j.reprotox.2010.11.003, ISSN: 1873-1708.

Bruner-Tran KL, Osteen KG. Developmental exposure to TCDD reduces fertility and negatively affects pregnancy outcomes across multiple generations. Reprod. Toxicol [print-electronic]. 2011 Apr; 31(3): 344-50. PMID: 20955784, PMCID: PMC3044210, PII: S0890-6238(10)00314-X, DOI: 10.1016/j.reprotox.2010.10.003, ISSN: 1873-1708.

Herington JL, Crispens MA, Carvalho-Macedo AC, Camargos AF, Lebovic DI, Bruner-Tran KL, Osteen KG. Development and prevention of postsurgical adhesions in a chimeric mouse model of experimental endometriosis. Fertil. Steril [print-electronic]. 2011 Mar 3/15/2011; 95(4): 1295-301.e1. PMID: 20934690, PMCID: PMC3038191, PII: S0015-0282(10)02547-1, DOI: 10.1016/j.fertnstert.2010.09.017, ISSN: 1556-5653.

Bruner-Tran KL, Osteen KG, Taylor HS, Sokalska A, Haines K, Duleba AJ. Resveratrol inhibits development of experimental endometriosis in vivo and reduces endometrial stromal cell invasiveness in vitro. Biol. Reprod [print-electronic]. 2011 Jan; 84(1): 106-12. PMID: 20844278, PMCID: PMC3012565, PII: biolreprod.110.086744, DOI: 10.1095/biolreprod.110.086744, ISSN: 1529-7268.

Bruner-Tran KL, Carvalho-Macedo AC, Duleba AJ, Crispens MA, Osteen KG. Experimental endometriosis in immunocompromised mice after adoptive transfer of human leukocytes. Fertil. Steril [print-electronic]. 2010 May 5/15/2010; 93(8): 2519-24. PMID: 19608172, PMCID: PMC2873129, PII: S0015-0282(09)01231-X, DOI: 10.1016/j.fertnstert.2009.05.076, ISSN: 1556-5653.

Bruner-Tran KL, Osteen KG. Dioxin-like PCBs and endometriosis. Syst Biol Reprod Med. 2010 Apr; 56(2): 132-46. PMID: 20377312, PMCID: PMC2867352, DOI: 10.3109/19396360903381023, ISSN: 1939-6376.

Krikun G, Hu Z, Osteen K, Bruner-Tran KL, Schatz F, Taylor HS, Toti P, Arcuri F, Konigsberg W, Garen A, Booth CJ, Lockwood CJ. The immunoconjugate "icon" targets aberrantly expressed endothelial tissue factor causing regression of endometriosis. Am. J. Pathol [print-electronic]. 2010 Feb; 176(2): 1050-6. PMID: 20042667, PMCID: PMC2808107, PII: S0002-9440(10)60414-2, DOI: 10.2353/ajpath.2010.090757, ISSN: 1525-2191.

Bruner-Tran KL, Ding T, Osteen KG. Dioxin and endometrial progesterone resistance. Semin. Reprod. Med [print-electronic]. 2010 Jan; 28(1): 59-68. PMID: 20104429, PMCID: PMC2875884, DOI: 10.1055/s-0029-1242995, ISSN: 1526-4564.

Bruner-Tran KL, Osteen KG, Duleba AJ. Simvastatin protects against the development of endometriosis in a nude mouse model. J. Clin. Endocrinol. Metab [print-electronic]. 2009 Jul; 94(7): 2489-94. PMID: 19366846, PMCID: PMC2708947, PII: jc.2008-2802, DOI: 10.1210/jc.2008-2802, ISSN: 1945-7197.

Bruner-Tran KL, Yeaman GR, Crispens MA, Igarashi TM, Osteen KG. Dioxin may promote inflammation-related development of endometriosis. Fertil. Steril [print-electronic]. 2008 May; 89(5 Suppl): 1287-98. PMID: 18394613, PMCID: PMC2430157, PII: S0015-0282(08)00367-1, DOI: 10.1016/j.fertnstert.2008.02.102, ISSN: 1556-5653.

Nayyar T, Bruner-Tran KL, Piestrzeniewicz-Ulanska D, Osteen KG. Developmental exposure of mice to TCDD elicits a similar uterine phenotype in adult animals as observed in women with endometriosis. Reprod. Toxicol [print-electronic]. 2007 Apr; 23(3): 326-36. PMID: 17056225, PMCID: PMC1885235, PII: S0890-6238(06)00238-3, DOI: 10.1016/j.reprotox.2006.09.007, ISSN: 0890-6238.

Bruner-Tran KL, Zhang Z, Eisenberg E, Winneker RC, Osteen KG. Down-regulation of endometrial matrix metalloproteinase-3 and -7 expression in vitro and therapeutic regression of experimental endometriosis in vivo by a novel nonsteroidal progesterone receptor agonist, tanaproget. J. Clin. Endocrinol. Metab [print-electronic]. 2006 Apr; 91(4): 1554-60. PMID: 16418212, PII: jc.2005-2024, DOI: 10.1210/jc.2005-2024, ISSN: 0021-972X.

Igarashi TM, Bruner-Tran KL, Yeaman GR, Lessey BA, Edwards DP, Eisenberg E, Osteen KG. Reduced expression of progesterone receptor-B in the endometrium of women with endometriosis and in cocultures of endometrial cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. Fertil. Steril. 2005 Jul; 84(1): 67-74. PMID: 16009159, PII: S0015-0282(05)00593-5, DOI: 10.1016/j.fertnstert.2005.01.113, ISSN: 1556-5653.

Osteen KG, Bruner-Tran KL, Eisenberg E. Endometrial biology and the etiology of endometriosis. Fertil. Steril. 2005 Jul; 84(1): 33-4; discussion 38. PMID: 16009151, PII: S0015-0282(05)00663-1, DOI: 10.1016/j.fertnstert.2005.01.124, ISSN: 1556-5653.

Kurita T, Medina R, Schabel AB, Young P, Gama P, Parekh TV, Brody J, Cunha GR, Osteen KG, Bruner-Tran KL, Gold LI. The activation function-1 domain of estrogen receptor alpha in uterine stromal cells is required for mouse but not human uterine epithelial response to estrogen. Differentiation. 2005 Jul; 73(6): 313-22. PMID: 16138832, PII: S0301-4681(09)60342-X, DOI: 10.1111/j.1432-0436.2005.00033.x, ISSN: 0301-4681.

Harris HA, Bruner-Tran KL, Zhang X, Osteen KG, Lyttle CR. A selective estrogen receptor-beta agonist causes lesion regression in an experimentally induced model of endometriosis. Hum. Reprod [print-electronic]. 2005 Apr; 20(4): 936-41. PMID: 15618247, PII: deh711, DOI: 10.1093/humrep/deh711, ISSN: 0268-1161.

Osteen KG, Bruner-Tran KL, Eisenberg E. Reduced progesterone action during endometrial maturation: a potential risk factor for the development of endometriosis. Fertil. Steril. 2005 Mar; 83(3): 529-37. PMID: 15749474, PII: S0015-0282(04)03187-5, DOI: 10.1016/j.fertnstert.2004.11.026, ISSN: 0015-0282.

Osteen KG, Igarashi TM, Yeaman GR, Bruner-Tran KL. Steroid and cytokine regulation of matrix metalloproteinases and the pathophysiology of endometriosis. Gynecol. Obstet. Invest. 2004; 57(1): 53-4. PMID: 14974460, ISSN: 0378-7346.

Fortin M, Lépine M, Pagé M, Osteen K, Massie B, Hugo P, Steff AM. An improved mouse model for endometriosis allows noninvasive assessment of lesion implantation and development. Fertil. Steril. 2003 Sep; 80 Suppl 2: 832-8. PMID: 14505761, PII: S0015028203009865, ISSN: 0015-0282.

Curry TE, Osteen KG. The matrix metalloproteinase system: changes, regulation, and impact throughout the ovarian and uterine reproductive cycle. Endocr. Rev. 2003 Aug; 24(4): 428-65. PMID: 12920150, DOI: 10.1210/er.2002-0005, ISSN: 0163-769X.

Hull ML, Charnock-Jones DS, Chan CL, Bruner-Tran KL, Osteen KG, Tom BD, Fan TP, Smith SK. Antiangiogenic agents are effective inhibitors of endometriosis. J. Clin. Endocrinol. Metab. 2003 Jun; 88(6): 2889-99. PMID: 12788903, DOI: 10.1210/jc.2002-021912, ISSN: 0021-972X.

Osteen KG, Yeaman GR, Bruner-Tran KL. Matrix metalloproteinases and endometriosis. Semin. Reprod. Med. 2003 May; 21(2): 155-64. PMID: 12917785, DOI: 10.1055/s-2003-41322, ISSN: 1526-8004.

Osteen KG, Igarashi TM, Bruner-Tran KL. Progesterone action in the human endometrium: induction of a unique tissue environment which limits matrix metalloproteinase (MMP) expression. Front. Biosci. 2003 Jan 1/1/2003; 8: d78-86. PMID: 12456342, ISSN: 1093-9946.

Kao LC, Germeyer A, Tulac S, Lobo S, Yang JP, Taylor RN, Osteen K, Lessey BA, Giudice, LC. Expression profiling of endometrium from women with endometriosis reveals candidate genes for disease-based implantation failure and infertility. Endocrinology. 2003; 144: 2870-81.

Fortin M, Lépine M, Pagé M, Osteen K, Massie B, Hugo P, Steff A-M. An improved mouse model for endometriosis allows noninvasive assessment of lesion implantation and development. Fertil and Steril. 2003; 80,Suppl 2: 832-8.

Hull ML, Charnock-Jones DS, Chan CL, Bruner-Tran KL, Osteen KG, Tom BD, Fan TP, Smith SK. Antiangiogenic agents are effective inhibitors of endometriosis. J Clin Endocrinol Metab. 2003; 88: 2889-99.

Apparao, KB, Illera MJ, Beyler SA, Olson GE, Osteen KG, Corjay MH, Boggess K, Lessey, BA. Regulated expression of osteopontin in the peri-implantation rabbit uterus. Biol Reprod. 2003; 68: 1484-90.

Bruner-Tran KL, Eisenberg E, Yeaman GR, Anderson TA, McBean J, Osteen KG. Steroid and cytokine regulation of matrix metalloproteinase expression in endometriosis and the establishment of experimental endometriosis in nude mice. J. Clin. Endocrinol. Metab. 2002 Oct; 87(10): 4782-91. PMID: 12364474, DOI: 10.1210/jc.2002-020418, ISSN: 0021-972X.

Osteen KG, Bruner-Tran KL, Keller NR, Eisenberg E. Progesterone-mediated endometrial maturation limits matrix metalloproteinase (MMP) expression in an inflammatory-like environment: a regulatory system altered in endometriosis. Ann. N. Y. Acad. Sci. 2002 Mar; 955: 37-47; discussion 86. PMID: 11949963, ISSN: 0077-8923.

Bruner-Tran KL, Webster-Clair D, Osteen KG. Experimental endometriosis: the nude mouse as a xenographic host. Ann. N. Y. Acad. Sci. 2002 Mar; 955: 328-39; discussion 340. PMID: 11949959, ISSN: 0077-8923.

Osteen KG, Bruner-Tran KL, Ong D, Eisenberg E. Paracrine mediators of endometrial matrix metalloproteinase expression: potential targets for progestin-based treatment of endometriosis. Ann. N. Y. Acad. Sci. 2002 Mar; 955: 139-46; discussion 157. PMID: 11949943, ISSN: 0077-8923.

Cornet PB, Picquet C, Lemoine P, Osteen KG, Bruner-Tran KL, Tabibzadeh S, Courtoy PJ, Eeckhout Y, Marbaix E, Henriet P. Regulation and function of LEFTY-A/EBAF in the human endometrium. mRNA expression during the menstrual cycle, control by progesterone, and effect on matrix metalloprotineases. Biol Chem. 2002; 277: 42496-504.

Bofinger DP, Feng L, Chi LH, Love J, Stephen FD, Sutter TR, Osteen KG, Costich TG, Batt RE, Koury ST, Olson JR. Effect of TCDD exposure on CYP1A1 and CYP1B1 expression in explant cultures of human endometrium. Toxicol Sci. 2001; 62: 299-314.

Curry TE Jr, Osteen KG. Cyclic changes in the matrix metalloproteinase system in the ovary and uterus. Biol Reprod. 2001; 64: 1285-96.

Keller NR, Sierra-Rivera E, Eisenberg E, Osteen KG. Progesterone exposure prevents matrix metalloproteinase-3 (MMP-3) stimulation by interleukin-1alpha in human endometrial stromal cells. J. Clin. Endocrinol. Metab. 2000 Apr; 85(4): 1611-9. PMID: 10770206, DOI: 10.1210/jcem.85.4.6502, ISSN: 0021-972X.

Zheng WL, Sierra-Rivera E, Luan J, Osteen KG, Ong DE. Retinoic acid synthesis and expression of cellular retinol-binding protein and cellular retinoic acid-binding protein type II are concurrent with decidualization of rat uterine stromal cells. Endocrinology. 2000 Feb; 141(2): 802-8. PMID: 10650963, DOI: 10.1210/endo.141.2.7323, ISSN: 0013-7227.

Bruner KL, Eisenberg E, Gorstein F, Osteen KG. Progesterone and transforming growth factor-beta coordinately regulate suppression of endometrial matrix metalloproteinases in a model of experimental endometriosis. Steroids. 1999 Sep; 64(9): 648-53. PMID: 10503723, PII: S0039-128X(99)00048-3, ISSN: 0039-128X.

Bruner-Tran KL, Rier SE, Eisenberg E, Osteen KG. The potential role of environmental toxins in the pathophysiology of endometriosis. Gynecol. Obstet. Invest. 1999; 48 Suppl 1: 45-56. PMID: 10559664, PII: 52868, DOI: 52868, ISSN: 0378-7346.

Osteen KG, Keller NR, Feltus FA, Melner MH. Paracrine regulation of matrix metalloproteinase expression in the normal human endometrium. Gynecol. Obstet. Invest. 1999; 48 Suppl 1: 2-13. PMID: 10559659, PII: 52863, DOI: 52863, ISSN: 0378-7346.

Zheng WL, Bucco RA, Sierra-Rievera E, Osteen KG, Melner MH, Ong DE. Synthesis of retinoic acid by rat ovarian cells that express cellular retinoic acid-binding protein-II. Biol. Reprod. 1999 Jan; 60(1): 110-4. PMID: 9858493, ISSN: 0006-3363.

Osteen KG, Sierra-Rivera E, Keller NR, Fox DB. Interleukin-1 alpha opposes progesterone-mediated suppression of MMP-7. A possible role of this cytokine during human implantation. Ann. N. Y. Acad. Sci. 1997 Sep 9/26/1997; 828: 137-45. PMID: 9329832, ISSN: 0077-8923.

Bruner KL, Matrisian LM, Rodgers WH, Gorstein F, Osteen KG. Suppression of matrix metalloproteinases inhibits establishment of ectopic lesions by human endometrium in nude mice. J. Clin. Invest. 1997 Jun 6/15/1997; 99(12): 2851-7. PMID: 9185507, PMCID: PMC508135, DOI: 10.1172/JCI119478, ISSN: 0021-9738.

Bucco RA, Zheng WL, Davis JT, Sierra-Rivera E, Osteen KG, Chaudhary AK, Ong DE. Cellular retinoic acid-binding protein(II) presence in rat uterine epithelial cells correlates with their synthesis of retinoic acid. Biochemistry. 1997 Apr 4/1/1997; 36(13): 4009-14. PMID: 9092831, PII: bi962094o, DOI: 10.1021/bi962094o, ISSN: 0006-2960.

Osteen KG, Sierra-Rivera E. Does disruption of immune and endocrine systems by environmental toxins contribute to development of endometriosis?. Semin. Reprod. Endocrinol. 1997; 15(3): 301-8. PMID: 9383839, DOI: 10.1055/s-2008-1068760, ISSN: 0734-8630.

Osteen KG, Bruner KL, Sharpe-Timms KL. Steroid and growth factor regulation of matrix metalloproteinase expression and endometriosis. Semin. Reprod. Endocrinol. 1996 Aug; 14(3): 247-55. PMID: 8885055, DOI: 10.1055/s-2007-1016334, ISSN: 0734-8630.

Bucco RA, Zheng WL, Wardlaw SA, Davis JT, Sierra-Rivera E, Osteen KG, Melner MH, Kakkad BP, Ong DE. Regulation and localization of cellular retinol-binding protein, retinol-binding protein, cellular retinoic acid-binding protein (CRABP), and CRABP II in the uterus of the pseudopregnant rat. Endocrinology. 1996 Jul; 137(7): 3111-22. PMID: 8770937, DOI: 10.1210/endo.137.7.8770937, ISSN: 0013-7227.

Bruner KL, Rodgers WH, Gold LI, Korc M, Hargrove JT, Matrisian LM, Osteen KG. Transforming growth factor beta mediates the progesterone suppression of an epithelial metalloproteinase by adjacent stroma in the human endometrium. Proc. Natl. Acad. Sci. U.S.A. 1995 Aug 8/1/1995; 92(16): 7362-6. PMID: 7638197, PMCID: PMC41339, ISSN: 0027-8424.

Osteen KG, Rodgers WH, Gaire M, Hargrove JT, Gorstein F, Matrisian LM. Stromal-epithelial interaction mediates steroidal regulation of metalloproteinase expression in human endometrium. Proc. Natl. Acad. Sci. U.S.A. 1994 Oct 10/11/1994; 91(21): 10129-33. PMID: 7937850, PMCID: PMC44971, ISSN: 0027-8424.

Rodgers, WH, Matrisian, LM, Giudice, LC, Dsupin, B, Cannon, P, Svitek, C, Gorstein, F, and KG Osteen. Patterns of matrix metalloproteinase expression in cycling endometrium imply differential functions and regulation by steroids. 1994.

Matrisian LM, Gaire M, Rodgers WH, Osteen KG. Metalloproteinase expression and hormonal regulation during tissue remodeling in the cycling human endometrium. Contrib Nephrol. 1994; 107: 94-100. PMID: 8004979, ISSN: 0302-5144.

Vasquez JM, Eisenberg E, Osteen KG, Hickerson D, Diamond MP. Laparoscopic ablation of endometriosis using the cavitational ultrasonic surgical aspirator. J Am Assoc Gynecol Laparosc. 1993 Nov; 1(1): 36-42. PMID: 9050458, ISSN: 1074-3804.

Bastias MC, Kamijo H, Osteen KG. Assessment of human sperm functional changes after in-vitro coincubation with cells retrieved from the human female reproductive tract. Hum. Reprod. 1993 Oct; 8(10): 1670-7. PMID: 8300826, ISSN: 0268-1161.

Rodgers WH, Osteen KG, Matrisian LM, Navre M, Giudice LC, Gorstein F. Expression and localization of matrilysin, a matrix metalloproteinase, in human endometrium during the reproductive cycle. Am. J. Obstet. Gynecol. 1993 Jan; 168(1 Pt 1): 253-60. PMID: 8420336, ISSN: 0002-9378.

Freeman MR, Bastias MC, Hill GA, Osteen KG. Coculture of mouse embryos with cells isolated from the human ovarian follicle, oviduct, and uterine endometrium. Fertil. Steril. 1993 Jan; 59(1): 138-42. PMID: 8419201, ISSN: 0015-0282.

Osteen KG, Anderson TL, Schwartz K, Hargrove JT, Gorstein F. Distribution of tumor-associated glycoprotein-72 (TAG-72) expression throughout the normal female reproductive tract. Int. J. Gynecol. Pathol. 1992 Jul; 11(3): 216-20. PMID: 1399229, ISSN: 0277-1691.

Hill GA, Osteen KG. The effect of insulin on in vitro progesterone production of human granulosa cells. Int. J. Fertil. 1992 Mar; 37(2): 93-8. PMID: 1349597, ISSN: 0020-725X.

Osteen KG, Anderson TL. Effect of estrogen on human endometrial epithelial cell growth and differentiation in vitro. Steroids. 1991 May; 56(5): 279-83. PMID: 1715101, ISSN: 0039-128X.

Segars JH, Hill GA, Bryan SH, Herbert CM, Osteen KG, Rogers BJ, Wentz AC. The use of gonadotropin releasing hormone agonist (GnRHa) in good responders undergoing repeat in vitro fertilization/embryo transfer (IVF/ET). J In Vitro Fert Embryo Transf. 1990 Dec; 7(6): 327-31. PMID: 2127603, ISSN: 0740-7769.

Osteen KG, Anderson TL, Hargrove JT, Hill GA, Gorstein F. Epithelial cells from normal human endometrium express a tumor-associated glycoprotein (TAG-72) epitope in vitro. Am. J. Obstet. Gynecol. 1990 Aug; 163(2): 479-84. PMID: 1696780, PII: 0002-9378(90)91179-G, ISSN: 0002-9378.

Anderson TL, Gorstein F, Osteen KG. Stromal-epithelial cell communication, growth factors, and tissue regulation. Lab. Invest. 1990 May; 62(5): 519-21. PMID: 2188045, ISSN: 0023-6837.

Herbert CM, Hill GA, Maxson WS, Wentz AC, Osteen KG. Use of a sensitive urine pregnancy test before endometrial biopsies taken in the late luteal phase. Fertil. Steril. 1990 Jan; 53(1): 162-4. PMID: 2295336, ISSN: 0015-0282.

Osteen KG, Hill GA, Hargrove JT, Gorstein F. Development of a method to isolate and culture highly purified populations of stromal and epithelial cells from human endometrial biopsy specimens. Fertil. Steril. 1989 Dec; 52(6): 965-72. PMID: 2687030, ISSN: 0015-0282.

Hill GA, Freeman M, Bastias MC, Rogers BJ, Herbert CM, Osteen KG, Wentz AC. The influence of oocyte maturity and embryo quality on pregnancy rate in a program for in vitro fertilization-embryo transfer. Fertil. Steril. 1989 Nov; 52(5): 801-6. PMID: 2509252, ISSN: 0015-0282.

Blackburn DG, Osteen KG, Winfrey VP, Hoffman LH. Obplacental giant cells of the domestic rabbit: development, morphology, and intermediate filament composition. J. Morphol. 1989 Nov; 202(2): 185-203. PMID: 2479758, DOI: 10.1002/jmor.1052020207, ISSN: 0362-2525.

Segars JH, Niblack GD, Osteen KG, Rogers BJ, Wentz AC. The human blastocyst produces a soluble factor(s) that interferes with lymphocyte proliferation. Fertil. Steril. 1989 Sep; 52(3): 381-7. PMID: 2528475, ISSN: 0015-0282.

Hill GA, Osteen KG. Follicular fluid steroid content and in vitro steroid secretion by granulosa-lutein cells from individual follicles among different stimulation protocols for in vitro fertilization-embryo transfer. J In Vitro Fert Embryo Transf. 1989 Aug; 6(4): 201-6. PMID: 2614215, ISSN: 0740-7769.

Aleshire SL, Osteen KG, Maxson WS, Entman SS, Bradley CA, Parl FF. Localization of transferrin and its receptor in ovarian follicular cells: morphologic studies in relation to follicular development. Fertil. Steril. 1989 Mar; 51(3): 444-9. PMID: 2646156, ISSN: 0015-0282.

Skinner MK, Osteen KG. Developmental and hormonal regulation of bovine granulosa cell function in the preovulatory follicle. Endocrinology. 1988 Sep; 123(3): 1668-75. PMID: 3402401, DOI: 10.1210/endo-123-3-1668, ISSN: 0013-7227.