Masakazu Shiota, D.V.M., Ph.D.

Associate Professor
Faculty Appointments
Associate Professor of Molecular Physiology and Biophysics
Ph.D., Veterinary Physiology, Osaka Prefecture University, Osaka, JapanM.S., Veterinary Physiology, Osaka Prefecture University, Osaka, JapanD.V.M., Ministry of Agriculture, Forestry and Fisheries, Tokyo, JapanB.S., Veterinary Science, Rakuno Gakuen University, Hokkaido, Japan
Office Address
762 Robinson Research Building
TN 0615
Research Description
The liver plays a central role in glucose homeostasis. During the postabsorptive and fasting period, >90% of glucose production is derived from the liver. When glucose enters the blood following feeding, the liver switches from net glucose production to net glucose uptake to lessen the rise in blood glucose. Such switching is regulated by raised blood glucose per se and plasma insulin. Impaired suppression of hepatic glucose production and a defect in hepatic glucose uptake in response to the raise in plasma glucose and insulin are major pathogenesis in fasting and excessive postprandial hyperglycemia that are common feature in obesity and diabetes. Our laboratory is interested in the mechanism of the unresponsiveness of hepatic glucose flux to changes in blood glucose and plasma insulin in obesity and diabetes.
Glucokinase (GK) plays a critical role in determining net hepatic glucose flux by catalyzing glucose phosphorylation, the first step of glucose utilization by the liver, and by opposing glucose-6-phosphatase, which catalyze glucose de-phosphorylation, the last step of glucose production by the liver. GK activity is regulated allosterically by its regulatory protein (GKRP). GKRP binds to the allosteric site in GK and inhibits GK decreasing the apparent affinity of the enzyme for glucose. We found that in normal rats, GK, but not GKRP, translocated rapidly from the nucleus to the cytoplasm in response to physiological increases in plasma glucose and/or insulin while both proteins co-located in the nucleus in the presence of basal levels of plasma glucose and insulin. We also found that the unresponsiveness of hepatic glucose flux to the rise in plasma glucose and/or insulin seen in Zucker diabetic fatty rats, type 2 diabetes model with obesity, was associated with impaired trasnlocation of GK in the early stage of diabetes and abnormal localization of GKRP in the cytoplasm together with GK in the later stage of diabetes.
To understand pathogenic role for altered regulation of GK activity by GKRP in impaired hepatic glucose flux in obesity and diabetes, our laboratory focuses on 1) mechanism by which the subcellular localization of GK and GKRP is determined in normal animals, 2) the way in which the GK translocation is impaired in the early stage of diabetes, 3) the relationship between altered distribution of GKRP and impaired glucose phosphorylation by GK in later stage of diabetes, and 4) effect of increasing free (unbound) GK by transfecting GK gene to improve impaired responsiveness of hepatic glucose flux to the rise in plasma glucose and/or insulin and to reduce hyperglycemia in diabetic animals.
Research Keywords
Hepatic Glucose Flux Regulation in Obesity and Type 2 Diabetes.
Pino MF, Kim KA, Shelton KD, Lindner J, Odili S, Li C, Collins HW, Shiota M, Matschinsky FM, Magnuson MA. Glucokinase thermolability and hepatic regulatory protein binding are essential factors for predicting the blood glucose phenotype of missense mutations. J. Biol. Chem [print-electronic]. 2007 May 5/4/2007; 282(18): 13906-16. PMID: 17353190, PII: M610094200, DOI: 10.1074/jbc.M610094200, ISSN: 0021-9258.

Shin JS, Torres TP, Catlin RL, Donahue EP, Shiota M. A defect in glucose-induced dissociation of glucokinase from the regulatory protein in Zucker diabetic fatty rats in the early stage of diabetes. Am. J. Physiol. Regul. Integr. Comp. Physiol [print-electronic]. 2007 Apr; 292(4): R1381-90. PMID: 17204595, PII: 00260.2006, DOI: 10.1152/ajpregu.00260.2006, ISSN: 0363-6119.

Brissova M, Shostak A, Shiota M, Wiebe PO, Poffenberger G, Kantz J, Chen Z, Carr C, Jerome WG, Chen J, Baldwin HS, Nicholson W, Bader DM, Jetton T, Gannon M, Powers AC. Pancreatic islet production of vascular endothelial growth factor--a is essential for islet vascularization, revascularization, and function. Diabetes. 2006 Nov; 55(11): 2974-85. PMID: 17065333, PII: 55/11/2974, DOI: 10.2337/db06-0690, ISSN: 0012-1797.

Fujimoto Y, Torres TP, Donahue EP, Shiota M. Glucose toxicity is responsible for the development of impaired regulation of endogenous glucose production and hepatic glucokinase in Zucker diabetic fatty rats. Diabetes. 2006 Sep; 55(9): 2479-90. PMID: 16936196, PII: 55/9/2479, DOI: 10.2337/db05-1511, ISSN: 0012-1797.

Uno K, Katagiri H, Yamada T, Ishigaki Y, Ogihara T, Imai J, Hasegawa Y, Gao J, Kaneko K, Iwasaki H, Ishihara H, Sasano H, Inukai K, Mizuguchi H, Asano T, Shiota M, Nakazato M, Oka Y. Neuronal pathway from the liver modulates energy expenditure and systemic insulin sensitivity. Science. 2006 Jun 6/16/2006; 312(5780): 1656-9. PMID: 16778057, PII: 312/5780/1656, DOI: 10.1126/science.1126010, ISSN: 1095-9203.

Fujitani Y, Fujitani S, Boyer DF, Gannon M, Kawaguchi Y, Ray M, Shiota M, Stein RW, Magnuson MA, Wright CV. Targeted deletion of a cis-regulatory region reveals differential gene dosage requirements for Pdx1 in foregut organ differentiation and pancreas formation. Genes Dev. 2006 Jan 1/15/2006; 20(2): 253-66. PMID: 16418487, PMCID: PMC1356115, PII: 20/2/253, DOI: 10.1101/gad.1360106, ISSN: 0890-9369.

Shiota C, Rocheleau JV, Shiota M, Piston DW, Magnuson MA. Impaired glucagon secretory responses in mice lacking the type 1 sulfonylurea receptor. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2005 Oct; 289(4): E570-7. PMID: 15941784, PII: 00102.2005, DOI: 10.1152/ajpendo.00102.2005, ISSN: 0193-1849.

Shiota M, Galassetti P, Igawa K, Neal DW, Cherrington AD. Inclusion of low amounts of fructose with an intraportal glucose load increases net hepatic glucose uptake in the presence of relative insulin deficiency in dog. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2005 Jun; 288(6): E1160-7. PMID: 15671083, PII: 00391.2004, DOI: 10.1152/ajpendo.00391.2004, ISSN: 0193-1849.

Jones JR, Barrick C, Kim KA, Lindner J, Blondeau B, Fujimoto Y, Shiota M, Kesterson RA, Kahn BB, Magnuson MA. Deletion of PPARgamma in adipose tissues of mice protects against high fat diet-induced obesity and insulin resistance. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2005 Apr 4/26/2005; 102(17): 6207-12. PMID: 15833818, PMCID: PMC556131, PII: 0306743102, DOI: 10.1073/pnas.0306743102, ISSN: 0027-8424.

Fowler M, Virostko J, Chen Z, Poffenberger G, Radhika A, Brissova M, Shiota M, Nicholson WE, Shi Y, Hirshberg B, Harlan DM, Jansen ED, Powers AC. Assessment of pancreatic islet mass after islet transplantation using in vivo bioluminescence imaging. Transplantation. 2005 Apr 4/15/2005; 79(7): 768-76. PMID: 15818318, PII: 00007890-200504150-00005, ISSN: 0041-1337.

Brissova M, Blaha M, Spear C, Nicholson W, Radhika A, Shiota M, Charron MJ, Wright CV, Powers AC. Reduced PDX-1 expression impairs islet response to insulin resistance and worsens glucose homeostasis. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2005 Apr; 288(4): E707-14. PMID: 15562255, PII: 00252.2004, DOI: 10.1152/ajpendo.00252.2004, ISSN: 0193-1849.

Fujimoto Y, Donahue EP, Shiota M. Defect in glucokinase translocation in Zucker diabetic fatty rats. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2004 Sep; 287(3): E414-23. PMID: 15138155, PII: 00575.2003, DOI: 10.1152/ajpendo.00575.2003, ISSN: 0193-1849.

Wang XL, Herzog B, Waltner-Law M, Hall RK, Shiota M, Granner DK. The synergistic effect of dexamethasone and all-trans-retinoic acid on hepatic phosphoenolpyruvate carboxykinase gene expression involves the coactivator p300. J. Biol. Chem [print-electronic]. 2004 Aug 8/13/2004; 279(33): 34191-200. PMID: 15166231, PII: M403455200, DOI: 10.1074/jbc.M403455200, ISSN: 0021-9258.

Brissova M, Fowler M, Wiebe P, Shostak A, Shiota M, Radhika A, Lin PC, Gannon M, Powers AC. Intraislet endothelial cells contribute to revascularization of transplanted pancreatic islets. Diabetes. 2004 May; 53(5): 1318-25. PMID: 15111502, ISSN: 0012-1797.

Chu CA, Fujimoto Y, Igawa K, Grimsby J, Grippo JF, Magnuson MA, Cherrington AD, Shiota M. Rapid translocation of hepatic glucokinase in response to intraduodenal glucose infusion and changes in plasma glucose and insulin in conscious rats. Am. J. Physiol. Gastrointest. Liver Physiol [print-electronic]. 2004 Apr; 286(4): G627-34. PMID: 14656711, PII: 00218.2003, DOI: 10.1152/ajpgi.00218.2003, ISSN: 0193-1857.

An J, Muoio DM, Shiota M, Fujimoto Y, Cline GW, Shulman GI, Koves TR, Stevens R, Millington D, Newgard CB.. Hepatic expression of Malonyl CoA decarboxylase reverses in muscle, liver and whole-animal insulin resistance in rats with diet-induced obesity. Nature Med. 2004; 10: 268-74.

Wu L, Nicholson W, Wu CY, Xu M, McGaha A, Shiota M, Powers AC. Engineering physiologically regulated insulin secretion in non-beta cells by expressing glucagon-like peptide 1 receptor. Gene Ther. 2003 Sep; 10(19): 1712-20. PMID: 12923570, PII: 3302055, DOI: 10.1038/, ISSN: 0969-7128.

Magnuson MA, She P, Shiota M. Gene-altered mice and metabolic flux control. J. Biol. Chem [print-electronic]. 2003 Aug 8/29/2003; 278(35): 32485-8. PMID: 12788933, PII: R300020200, DOI: 10.1074/jbc.R300020200, ISSN: 0021-9258.

She P, Burgess SC, Shiota M, Flakoll P, Donahue EP, Malloy CR, Sherry AD, Magnuson MA. Mechanisms by which liver-specific PEPCK knockout mice preserve euglycemia during starvation. Diabetes. 2003 Jul; 52(7): 1649-54. PMID: 12829628, ISSN: 0012-1797.

Nishizawa M, Moore MC, Shiota M, Gustavson SM, Snead WL, Neal DW, Cherrington AD. Effect of intraportal glucagon-like peptide-1 on glucose metabolism in conscious dogs. Am. J. Physiol. Endocrinol. Metab [print-electronic]. 2003 May; 284(5): E1027-36. PMID: 12569088, PII: 00503.2002, DOI: 10.1152/ajpendo.00503.2002, ISSN: 0193-1849.

Brissova M, Nicholson WE, Shiota M, Powers AC. Assessment of insulin secretion in the mouse. Methods Mol. Med. 2003; 83: 23-45. PMID: 12619713, DOI: 10.1385/1-59259-377-1:023, ISSN: 1543-1894.

Shiota C, Larsson O, Shelton KD, Shiota M, Efanov AM, Hoy M, Lindner J, Kooptiwut S, Juntti-Berggren L, Gromada J, Berggren PO, Magnuson MA. Sulfonylurea receptor type 1 knock-out mice have intact feeding-stimulated insulin secretion despite marked impairment in their response to glucose. J. Biol. Chem [print-electronic]. 2002 Oct 10/4/2002; 277(40): 37176-83. PMID: 12149271, PII: M206757200, DOI: 10.1074/jbc.M206757200, ISSN: 0021-9258.

Igawa K, Mugavero M, Shiota M, Neal DW, Cherrington AD. Insulin sensitively controls the glucagon response to mild hypoglycemia in the dog. Diabetes. 2002 Oct; 51(10): 3033-42. PMID: 12351444, ISSN: 0012-1797.

Brissova M, Shiota M, Nicholson WE, Gannon M, Knobel SM, Piston DW, Wright CV, Powers AC. Reduction in pancreatic transcription factor PDX-1 impairs glucose-stimulated insulin secretion. J. Biol. Chem [print-electronic]. 2002 Mar 3/29/2002; 277(13): 11225-32. PMID: 11781323, PII: M111272200, DOI: 10.1074/jbc.M111272200, ISSN: 0021-9258.

Shiota M, Moore MC, Galassetti P, Monohan M, Neal DW, Shulman GI, Cherrington AD. Inclusion of low amounts of fructose with an intraduodenal glucose load markedly reduces postprandial hyperglycemia and hyperinsulinemia in the conscious dog. Diabetes. 2002 Feb; 51(2): 469-78. PMID: 11812757, ISSN: 0012-1797.

Sherck SM, Shiota M, Saccomando J, Cardin S, Allen EJ, Hastings JR, Neal DW, Williams PE, Cherrington AD. Pancreatic response to mild non-insulin-induced hypoglycemia does not involve extrinsic neural input. Diabetes. 2001 Nov; 50(11): 2487-96. PMID: 11679426, ISSN: 0012-1797.

Shiota M, Postic C, Fujimoto Y, Jetton TL, Dixon K, Pan D, Grimsby J, Grippo JF, Magnuson MA, Cherrington AD. Glucokinase gene locus transgenic mice are resistant to the development of obesity-induced type 2 diabetes. Diabetes. 2001 Mar; 50(3): 622-9. PMID: 11246883, ISSN: 0012-1797.

Flattem N, Igawa K, Shiota M, Emshwiller MG, Neal DW, Cherrington AD. Alpha- and beta-cell responses to small changes in plasma glucose in the conscious dog. Diabetes. 2001 Feb; 50(2): 367-75. PMID: 11272149, ISSN: 0012-1797.

Jetton TL, Shiota M, Knobel SM, Piston DW, Cherrington AD, Magnuson MA. Substrate-induced nuclear export and peripheral compartmentalization of hepatic glucokinase correlates with glycogen deposition. Int. J. Exp. Diabetes Res. 2001; 2(3): 173-86. PMID: 12369705, PMCID: PMC2478546, ISSN: 1560-4284.

Postic C, Shiota M, Magnuson MA. Cell-specific roles of glucokinase in glucose homeostasis. Recent Prog. Horm. Res. 2001; 56: 195-217. PMID: 11237213, ISSN: 0079-9963.

She P, Shiota M, Shelton KD, Chalkley R, Postic C, Magnuson MA. Phosphoenolpyruvate carboxykinase is necessary for the integration of hepatic energy metabolism. Mol. Cell. Biol. 2000 Sep; 20(17): 6508-17. PMID: 10938127, PMCID: PMC86125, ISSN: 0270-7306.

Shiota M, Jackson P, Galassetti P, Scott M, Neal DW, Cherrington AD. Combined intraportal infusion of acetylcholine and adrenergic blockers augments net hepatic glucose uptake. Am. J. Physiol. Endocrinol. Metab. 2000 Mar; 278(3): E544-52. PMID: 10710510, ISSN: 0193-1849.

Lu JT, Son YJ, Lee J, Jetton TL, Shiota M, Moscoso L, Niswender KD, Loewy AD, Magnuson MA, Sanes JR, Emeson RB. Mice lacking alpha-calcitonin gene-related peptide exhibit normal cardiovascular regulation and neuromuscular development. Mol. Cell. Neurosci. 1999 Aug; 14(2): 99-120. PMID: 10532808, PII: S1044-7431(99)90767-4, DOI: 10.1006/mcne.1999.0767, ISSN: 1044-7431.

Postic C, Shiota M, Niswender KD, Jetton TL, Chen Y, Moates JM, Shelton KD, Lindner J, Cherrington AD, Magnuson MA. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. J. Biol. Chem. 1999 Jan 1/1/1999; 274(1): 305-15. PMID: 9867845, ISSN: 0021-9258.

Galassetti P, Shiota M, Zinker BA, Wasserman DH, Cherrington AD. A negative arterial-portal venous glucose gradient decreases skeletal muscle glucose uptake. Am. J. Physiol. 1998 Jul; 275(1 Pt 1): E101-11. PMID: 9688880, ISSN: 0002-9513.

Shiota M, Galassetti P, Monohan M, Neal DW, Cherrington AD. Small amounts of fructose markedly augment net hepatic glucose uptake in the conscious dog. Diabetes. 1998 Jun; 47(6): 867-73. PMID: 9604861, ISSN: 0012-1797.

Kimura K, Moriyama M, Nishisako M, Kannan Y, Shiota M, Sugano T. Endotoxin modulates arachidonic acid-induced glycogenolysis in the perfused rat liver. Horm. Metab. Res. 1998 Apr; 30(4): 178-81. PMID: 9623630, DOI: 10.1055/s-2007-978862, ISSN: 0018-5043.

Kimura K, Moriyama M, Nishisako M, Kannan Y, Shiota M, Sakurada K, Musashi M, Sugano T. Modulation of platelet activating factor-induced glycogenolysis in the perfused rat liver after administration of endotoxin in vivo. J. Biochem. 1998 Jan; 123(1): 142-9. PMID: 9504421, ISSN: 0021-924X.

Jackson PA, Pagliassotti MJ, Shiota M, Neal DW, Cardin S, Cherrington AD. Effects of vagal blockade on the counterregulatory response to insulin-induced hypoglycemia in the dog. Am. J. Physiol. 1997 Dec; 273(6 Pt 1): E1178-88. PMID: 9435534, ISSN: 0002-9513.

Shiota M, Jackson PA, Bischoff H, McCaleb M, Scott M, Monohan M, Neal DW, Cherrington AD. Inhibition of glycogenolysis enhances gluconeogenic precursor uptake by the liver of conscious dogs. Am. J. Physiol. 1997 Nov; 273(5 Pt 1): E868-79. PMID: 9374671, ISSN: 0002-9513.

Niswender KD, Shiota M, Postic C, Cherrington AD, Magnuson MA. Effects of increased glucokinase gene copy number on glucose homeostasis and hepatic glucose metabolism. J. Biol. Chem. 1997 Sep 9/5/1997; 272(36): 22570-5. PMID: 9278411, ISSN: 0021-9258.

Okuno A, Ikeda K, Shiota M, Fujiwara T, Yoshioka S, Sugano T, Horikoshi H. Acute effect of troglitazone on glucose metabolism in the absence or presence of insulin in perfused rat hindlimb. Metab. Clin. Exp. 1997 Jun; 46(6): 716-21. PMID: 9186311, ISSN: 0026-0495.

Niswender KD, Postic C, Shiota M, Jetton TL, Magnuson MA. Effects of altered glucokinase gene copy number on blood glucose homoeostasis. Biochem. Soc. Trans. 1997 Feb; 25(1): 113-7. PMID: 9056854, ISSN: 0300-5127.

Kimura K, Hamada M, Moriyama M, Kannan Y, Shiota M, Sakurada K, Musashi M, Sugano T. Phorbol ester, but not endotoxin, desensitizes mannan-induced glycogenolysis in the perfused rat liver. J. Biochem. 1996 Sep; 120(3): 488-93. PMID: 8902610, ISSN: 0021-924X.

Moriyama M, Makiyama I, Shiota M, Uesugi K, Kannan Y, Ohta M, Kimura K, Sugano T. Decreased ureagenesis from alanine, but not from ammonia and glutamine, in the perfused rat liver after partial hepatectomy. Hepatology. 1996 Jun; 23(6): 1584-90. PMID: 8675181, PII: S0270-9139(96)00251-0, DOI: 10.1053/jhep.1996.v23.pm0008675181, ISSN: 0270-9139.

Shiota M, Green R, Colburn CA, Mitchell G, Cherrington AD. Inability of hyperglycemia to counter the ability of glucagon to increase net glucose output and activate glycogen phosphorylase in the perfused rat liver. Metab. Clin. Exp. 1996 Apr; 45(4): 481-5. PMID: 8609835, ISSN: 0026-0495.

Kimura K, Okamoto Y, Sashiwa H, Saimoto H, Shigemasa Y, Moriyama M, Shiota M, Sugano T. Partially deacetylated chitin and hyaluronan induce glycogenolysis in perfused rat liver. J. Biochem. 1995 Nov; 118(5): 953-8. PMID: 8749312, ISSN: 0021-924X.

Shiota M, Inagami M, Fujimoto Y, Moriyama M, Kimura K, Sugano T. Cold acclimation induces zonal heterogeneity in gluconeogenic responses to glucagon in rat liver lobule. Am. J. Physiol. 1995 Jun; 268(6 Pt 1): E1184-91. PMID: 7611395, ISSN: 0002-9513.

Shiota M, Chinzei F, Moriyama M, Kimura K, Sugano T. Effects of an increase in perfusion pressure and of the direction of flow on carbohydrate metabolism in the perfused rat liver. J. Biochem. 1995 May; 117(5): 958-64. PMID: 8586640, ISSN: 0021-924X.

Shiota M, Fujimoto J, Takenaga M, Satoh H, Ichinohasama R, Abe M, Nakano M, Yamamoto T, Mori S. Diagnosis of t(2;5)(p23;q35)-associated Ki-1 lymphoma with immunohistochemistry. Blood. 1994 Dec 12/1/1994; 84(11): 3648-52. PMID: 7949120, ISSN: 0006-4971.

Shiota M, Hiramatsu M, Fujimoto Y, Moriyama M, Kimura K, Ohta M, Sugano T. The capacity of the malate-aspartate shuttle differs between periportal and perivenous hepatocytes from rats. Arch. Biochem. Biophys. 1994 Feb 2/1/1994; 308(2): 349-56. PMID: 8109964, PII: S0003-9861(84)71050-2, DOI: 10.1006/abbi.1994.1050, ISSN: 0003-9861.

Shiota M, Fujimoto Y, Inagami M, Hiramatsu M, Moriyama M, Kimura K, Ohta M, Sugano T. Adaptive changes in zonation for gluconeogenic capacity in liver lobules of cold-exposed rats. Am. J. Physiol. 1993 Oct; 265(4 Pt 1): E559-64. PMID: 8238330, ISSN: 0002-9513.

Matsui Y, Ishibashi H, Kimura K, Shiota M, Ohta M, Sugano T. Responses to catecholamines in perfused livers of hypothalamic-lesioned rats. Am. J. Physiol. 1993 Jul; 265(1 Pt 2): R117-23. PMID: 8342675, ISSN: 0002-9513.

Shiota M, Kurano Y, Mochizuki Y, Kimura K, Ohta M, Sugano T. Effects of nerve stimulation and zymosan on glycogenolysis in perfused livers from cold-exposed rats. Am. J. Physiol. 1992 Sep; 263(3 Pt 1): G353-9. PMID: 1415548, ISSN: 0002-9513.

Kimura K, Shiota M, Mochizuki K, Ohta M, Sugano T. Different preparations of zymosan induce glycogenolysis independently in the perfused rat liver. Involvement of mannose receptors, peptide-leukotrienes and prostaglandins. Biochem. J. 1992 May 5/1/1992; 283 ( Pt 3): 773-9. PMID: 1317164, PMCID: PMC1130953, ISSN: 0264-6021.

Hamatani Y, Inoue M, Kimura K, Shiota M, Ohta M, Sugano T. Effects of calmodulin antagonists on hydrogen-translocating shuttles in perfused rat liver. Am. J. Physiol. 1991 Sep; 261(3 Pt 1): E325-31. PMID: 1887879, ISSN: 0002-9513.

Noda K, Shiota M, Tanizawa O, Inoue M, Tenjin Y, Sugishita T, Yakushiji M, Miyoshi T, Nozawa S, Sakuma T. [Clinical significance of serum sialyl Tn antigen in patients with gynecological cancer. STN Study Group]. Gan To Kagaku Ryoho. 1991 Jul; 18(8): 1287-96. PMID: 2069400, ISSN: 0385-0684.

Kobayashi T, Iwai H, Uchimoto R, Ohta M, Shiota M, Sugano T. Gluconeogenesis in perfused livers from dexamethasone-treated chickens. Am. J. Physiol. 1989 Apr; 256(4 Pt 2): R907-14. PMID: 2705579, ISSN: 0002-9513.

Sugano T, Nishimura K, Sogabe N, Shiota M, Oyama N, Noda S, Ohta M. Ca2+-dependent activation of the malate-aspartate shuttle by norepinephrine and vasopressin in perfused rat liver. Arch. Biochem. Biophys. 1988 Jul; 264(1): 144-54. PMID: 2899418, PII: 0003-9861(88)90579-6, ISSN: 0003-9861.

Shiota M, Masumi S. Effect of norepinephrine on consumption of oxygen in perfused skeletal muscle from cold-exposed rats. Am. J. Physiol. 1988 Apr; 254(4 Pt 1): E482-9. PMID: 3354663, ISSN: 0002-9513.

Yamada T, Shiota M, Sugano T. Effects of cold exposure on inhibition by isoproterenol of release of alanine in the rat. J. Appl. Physiol. 1988 Mar; 64(3): 906-12. PMID: 2835350, ISSN: 8750-7587.

Yamano T, Yorita K, Fujii H, Uchimoto R, Shiota M, Ohta M, Sugano T. Gluconeogenesis in perfused chicken kidney. Effects of feeding and starvation. Comp. Biochem. Physiol., B. 1988; 91(4): 701-6. PMID: 3224508, ISSN: 0305-0491.

Yorita K, Yamano T, Ikeda K, Kobayashi T, Shiota M, Sugano T. Distribution of glycolysis and gluconeogenesis in perfused chicken kidney. Am. J. Physiol. 1987 Nov; 253(5 Pt 2): R679-86. PMID: 3688272, ISSN: 0002-9513.

Shiota M, Sugano T. Characteristics of rat hindlimbs perfused with erythrocyte- and albumin-free medium. Am. J. Physiol. 1986 Jul; 251(1 Pt 1): C78-84. PMID: 3524256, ISSN: 0002-9513.

Shiota M, Tanaka T, Sugano T. Effect of norepinephrine on gluconeogenesis in perfused livers of cold-exposed rats. Am. J. Physiol. 1985 Sep; 249(3 Pt 1): E281-6. PMID: 4037079, ISSN: 0002-9513.

Shiota M, Sugano T. [Mechanisms involved in metabolic responses to cold exposure]. Seikagaku. 1985 Jan; 57(1): 36-47. PMID: 3889179, ISSN: 0037-1017.

Shiota M, Golden S, Katz J. Lactate metabolism in the perfused rat hindlimb. Biochem. J. 1984 Sep 9/1/1984; 222(2): 281-92. PMID: 6383357, PMCID: PMC1144177, ISSN: 0264-6021.

Sugano T, Shiota M, Khono H, Shimada M. Stimulation of gluconeogenesis by glucagon and norepinephrine in the perfused chicken liver. J. Biochem. 1982 Jul; 92(1): 111-20. PMID: 7118865, ISSN: 0021-924X.

Sugano T, Shiota M, Khono H, Shimada M. Intracellular redox state and control of gluconeogenesis in perfused chicken liver. J. Biochem. 1982 Jun; 91(6): 1917-29. PMID: 7118853, ISSN: 0021-924X.

Sugano T, Shiota M, Khono H, Shimada M, Oshino N. Effects of calcium ions on the activation of gluconeogenesis by norepinephrine in perfused rat liver. J. Biochem. 1980 Feb; 87(2): 465-72. PMID: 7358649, ISSN: 0021-924X.

Sugano T, Shiota M, Tanaka T, Miyamae Y, Shimada M, Oshino N. Intracellular redox state and stimulation of gluconeogenesis by glucagon and norepinephrine in the perfused rat liver. J. Biochem. 1980 Jan; 87(1): 153-66. PMID: 7358625, ISSN: 0021-924X.