David Cortez, Ph.D.



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Faculty Appointments
Chair, Department of Biochemistry Professor of Biochemistry Richard N. Armstrong, Ph.D. Chair for Innovation in BiochemistryIngram Professor of Cancer Research
Ph.D., Molecular Cancer Biology, Duke University, Durham, North CarolinaB.S., Biochemistry, University of Illinois, Urbana-Champaign, Illinois
Office Address
613B Light Hall
TN 0146
Research Description
Billions of base pairs of DNA must be replicated trillions of times during a human lifetime. Adding to the difficulty, thousands of DNA lesions happen in each cell of our body every day. Furthermore, replication is challenged by difficult to replicate sequences and conflicts with transcription. DNA damage response mechanisms act to repair the damaged DNA, signal checkpoint activation, ensure completion of DNA replication, and maintain genome stability. Defects in these mechanisms can cause developmental abnormalities, premature aging, and cancer.

The Cortez lab is dedicated to understanding the mechanisms that maintain genome integrity and promote the complete and accurate replication of the genome.

Current projects in the lab include:

1. Discovery of new replication, repair, and DNA damage response proteins using genetic screens and proteomics
2. Characterization of replication fork remodeling and fork protection proteins related to the BRCA1/BRCA2 pathway
3. Understanding sources of genome instability in normal and cancer cells
4. Analysis of DNA damage signaling pathways controlled by the ATR kinase
5. Development of cancer therapeutic approaches targeting the DNA damage response
6. Characterization of mechanisms that repair abasic sites generated by endogenous and environmental mutagens

We use a multi-disciplinary approach including genetics, biochemistry, cell biology, proteomics, and structural biology. The lab is part of the Genome Maintenance Program within the Vanderbilt-Ingram Cancer Center, Center in Molecular Toxicology, Vanderbilt Institute of Chemical Biology, and Department of Biochemistry.

Since DNA damage is continuously produced as a byproduct of normal cell metabolism and DNA replication, any deficiency in responding to and repairing this damage can cause chromosomal alterations that may lead to cancer, developmental abnormalities, and other diseases. In addition many cancer therapies including radiation therapy and most chemotherapeutic strategies cause DNA damage. Therefore, manipulating the DNA damage response may be one means of improving the outcomes of these therapies. Discovering the mechanisms by which cells respond to DNA damage and other types of replication stress will provide insights into the etiology of diseases like cancer as well as identify opportunities for therapeutic intervention.

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Research Keywords
Genome maintenance by the DNA damage response, cell cycle, DNA replication, DNA repair, cancer, proteomics, cell biology, genome editing, biochemistry, genetics, mass spectrometry, CRISPR-Cas9, signaling, kinase, toxicology, chemical biology
Paulin KA, Cortez D, Eichman BF. The SOS response-associated peptidase (SRAP) domain of YedK catalyzes ring opening of abasic sites and reversal of its DNA-protein crosslink. J Biol Chem [print-electronic]. 2022 Aug 8/4/2022; 102307. PMID: 35934051, PII: S0021-9258(22)00749-9, DOI: 10.1016/j.jbc.2022.102307, ISSN: 1083-351X.

Van Ravenstein SX, Mehta KP, Kavlashvili T, Byl JAW, Zhao R, Osheroff N, Cortez D, Dewar JM. Topoisomerase II poisons inhibit vertebrate DNA replication through distinct mechanisms. EMBO J [print-electronic]. 2022 Jun 6/14/2022; 41(12): e110632. PMID: 35578785, PMCID: PMC9194788, DOI: 10.15252/embj.2022110632, ISSN: 1460-2075.

Mehta KPM, Thada V, Zhao R, Krishnamoorthy A, Leser M, Lindsey Rose K, Cortez D. CHK1 phosphorylates PRIMPOL to promote replication stress tolerance. Sci Adv [print-electronic]. 2022 Apr; 8(13): eabm0314. PMID: 35353580, PMCID: PMC8967226, DOI: 10.1126/sciadv.abm0314, ISSN: 2375-2548.

Mohamed T, Adolph MB, Cortez D. Oligomerization of DNA replication regulatory protein RADX is essential to maintain replication fork stability. J Biol Chem [print-electronic]. 2022 Mar; 298(3): 101672. PMID: 35120927, PMCID: PMC8902620, PII: S0021-9258(22)00112-0, DOI: 10.1016/j.jbc.2022.101672, ISSN: 1083-351X.

Krishnamoorthy A, Jackson J, Mohamed T, Adolph M, Vindigni A, Cortez D. RADX prevents genome instability by confining replication fork reversal to stalled forks. Mol Cell [print-electronic]. 2021 May 5/29/2021; PMID: 34107305, PII: S1097-2765(21)00390-7, DOI: 10.1016/j.molcel.2021.05.014, ISSN: 1097-4164.

Adolph MB, Mohamed TM, Balakrishnan S, Xue C, Morati F, Modesti M, Greene EC, Chazin WJ, Cortez D. RADX controls RAD51 filament dynamics to regulate replication fork stability. Mol Cell [print-electronic]. 2021 Mar 3/4/2021; 81(5): 1074-1083.e5. PMID: 33453169, PMCID: PMC7935748, PII: S1097-2765(20)30957-6, DOI: 10.1016/j.molcel.2020.12.036, ISSN: 1097-4164.

Thada V, Cortez D. ATR activation is regulated by dimerization of ATR activating proteins. J Biol Chem [print-electronic]. 2021 Feb 2/23/2021; 100455. PMID: 33636182, PMCID: PMC7994790, PII: S0021-9258(21)00228-3, DOI: 10.1016/j.jbc.2021.100455, ISSN: 1083-351X.

Liu W, Krishnamoorthy A, Zhao R, Cortez D. Two replication fork remodeling pathways generate nuclease substrates for distinct fork protection factors. Sci Adv [electronic-print]. 2020 Nov; 6(46): PMID: 33188024, PMCID: PMC7673757, PII: 6/46/eabc3598, DOI: 10.1126/sciadv.abc3598, ISSN: 2375-2548.

Berti M, Cortez D, Lopes M. The plasticity of DNA replication forks in response to clinically relevant genotoxic stress. Nat Rev Mol Cell Biol [print-electronic]. 2020 Oct; 21(10): 633-51. PMID: 32612242, PII: 10.1038/s41580-020-0257-5, DOI: 10.1038/s41580-020-0257-5, ISSN: 1471-0080.

Berti M, Cortez D, Lopes M. The plasticity of DNA replication forks in response to clinically relevant genotoxic stress. Nat. Rev. Mol. Cell Biol [print-electronic]. 2020 Jul 7/1/2020; PMID: 32612242, PII: 10.1038/s41580-020-0257-5, DOI: 10.1038/s41580-020-0257-5, ISSN: 1471-0080.

Mehta KPM, Lovejoy CA, Zhao R, Heintzman DR, Cortez D. HMCES Maintains Replication Fork Progression and Prevents Double-Strand Breaks in Response to APOBEC Deamination and Abasic Site Formation. Cell Rep. 2020 Jun 6/2/2020; 31(9): 107705. PMID: 32492421, PMCID: PMC7313144, PII: S2211-1247(20)30675-6, DOI: 10.1016/j.celrep.2020.107705, ISSN: 2211-1247.

Mehta KPM, Lovejoy CA, Zhao R, Heintzman DR, Cortez D. HMCES Maintains Replication Fork Progression and Prevents Double-Strand Breaks in Response to APOBEC Deamination and Abasic Site Formation. Cell Rep. 2020 Jun 6/2/2020; 31(9): 107705. PMID: 32492421, PMCID: PMC7313144, PII: S2211-1247(20)30675-6, DOI: 10.1016/j.celrep.2020.107705, ISSN: 2211-1247.

Thompson PS, Cortez D. New insights into abasic site repair and tolerance. DNA Repair (Amst.) [print-electronic]. 2020 Jun; 90: 102866. PMID: 32417669, PMCID: PMC7299775, PII: S1568-7864(20)30114-2, DOI: 10.1016/j.dnarep.2020.102866, ISSN: 1568-7856.

Thompson PS, Cortez D. New insights into abasic site repair and tolerance. DNA Repair (Amst) [print-electronic]. 2020 Jun; 90: 102866. PMID: 32417669, PMCID: PMC7299775, PII: S1568-7864(20)30114-2, DOI: 10.1016/j.dnarep.2020.102866, ISSN: 1568-7856.

Wessel SR, Mohni KN, Luzwick JW, Dungrawala H, Cortez D. Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators. Cell Rep. 2019 Sep 9/24/2019; 28(13): 3497-3509.e4. PMID: 31553917, PII: S2211-1247(19)31099-X, DOI: 10.1016/j.celrep.2019.08.051, ISSN: 2211-1247.

Thompson PS, Amidon KM, Mohni KN, Cortez D, Eichman BF. Protection of abasic sites during DNA replication by a stable thiazolidine protein-DNA cross-link. Nat. Struct. Mol. Biol [print-electronic]. 2019 Jun 6/24/2019; PMID: 31235915, PII: 10.1038/s41594-019-0255-5, DOI: 10.1038/s41594-019-0255-5, ISSN: 1545-9985.

Cortez D. Replication-Coupled DNA Repair. Mol. Cell. 2019 Jun 6/6/2019; 74(5): 866-76. PMID: 31173722, PMCID: PMC6557297, PII: S1097-2765(19)30319-3, DOI: 10.1016/j.molcel.2019.04.027, ISSN: 1097-4164.

Thada V, Cortez D. Common motifs in ETAA1 and TOPBP1 required for ATR kinase activation. J. Biol. Chem [print-electronic]. 2019 May 5/24/2019; 294(21): 8395-402. PMID: 30940728, PMCID: PMC6544864, PII: RA119.008154, DOI: 10.1074/jbc.RA119.008154, ISSN: 1083-351X.

Bass TE, Cortez D. Quantitative phosphoproteomics reveals mitotic function of the ATR activator ETAA1. J. Cell Biol [print-electronic]. 2019 Feb 2/12/2019; PMID: 30755469, PII: jcb.201810058, DOI: 10.1083/jcb.201810058, ISSN: 1540-8140.

Mohni KN, Wessel SR, Zhao R, Wojciechowski AC, Luzwick JW, Layden H, Eichman BF, Thompson PS, Mehta KPM, Cortez D. HMCES Maintains Genome Integrity by Shielding Abasic Sites in Single-Strand DNA. Cell [print-electronic]. 2019 Jan 1/10/2019; 176(1-2): 144-153.e13. PMID: 30554877, PMCID: PMC6329640, PII: S0092-8674(18)31454-5, DOI: 10.1016/j.cell.2018.10.055, ISSN: 1097-4172.

Saldivar JC, Hamperl S, Bocek MJ, Chung M, Bass TE, Cisneros-Soberanis F, Samejima K, Xie L, Paulson JR, Earnshaw WC, Cortez D, Meyer T, Cimprich KA. An intrinsic S/G2 checkpoint enforced by ATR. Science. 2018 Aug 8/24/2018; 361(6404): 806-10. PMID: 30139873, PMCID: PMC6365305, PII: 361/6404/806, DOI: 10.1126/science.aap9346, ISSN: 1095-9203.

Bhat KP, Krishnamoorthy A, Dungrawala H, Garcin EB, Modesti M, Cortez D. RADX Modulates RAD51 Activity to Control Replication Fork Protection. Cell Rep. 2018 Jul 7/17/2018; 24(3): 538-45. PMID: 30021152, PMCID: PMC6086571, PII: S2211-1247(18)30988-4, DOI: 10.1016/j.celrep.2018.06.061, ISSN: 2211-1247.

Carvajal-Maldonado D, Byrum AK, Jackson J, Wessel S, Lemaçon D, Guitton-Sert L, Quinet A, Tirman S, Graziano S, Masson JY, Cortez D, Gonzalo S, Mosammaparast N, Vindigni A. Perturbing cohesin dynamics drives MRE11 nuclease-dependent replication fork slowing. Nucleic Acids Res [print-electronic]. 2018 Jun 6/18/2018; PMID: 29917110, PII: 5039657, DOI: 10.1093/nar/gky519, ISSN: 1362-4962.

Bhat KP, Cortez D. RPA and RAD51: fork reversal, fork protection, and genome stability. Nat. Struct. Mol. Biol [print-electronic]. 2018 Jun; 25(6): 446-53. PMID: 29807999, PMCID: PMC6006513, PII: 10.1038/s41594-018-0075-z, DOI: 10.1038/s41594-018-0075-z, ISSN: 1545-9985.

Poole LA, Cortez D. Functions of SMARCAL1, ZRANB3, and HLTF in maintaining genome stability. Crit. Rev. Biochem. Mol. Biol [print-electronic]. 2017 Dec; 52(6): 696-714. PMID: 28954549, PMCID: PMC5962345, DOI: 10.1080/10409238.2017.1380597, ISSN: 1549-7798.

Saldivar JC, Cortez D, Cimprich KA. The essential kinase ATR: ensuring faithful duplication of a challenging genome. Nat. Rev. Mol. Cell Biol [print-electronic]. 2017 Oct; 18(10): 622-36. PMID: 28811666, PMCID: PMC5796526, PII: nrm.2017.67, DOI: 10.1038/nrm.2017.67, ISSN: 1471-0080.

Vujanovic M, Krietsch J, Raso MC, Terraneo N, Zellweger R, Schmid JA, Taglialatela A, Huang JW, Holland CL, Zwicky K, Herrador R, Jacobs H, Cortez D, Ciccia A, Penengo L, Lopes M. Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity. Mol. Cell. 2017 Sep 9/7/2017; 67(5): 882-890.e5. PMID: 28886337, PMCID: PMC5594246, PII: S1097-2765(17)30607-X, DOI: 10.1016/j.molcel.2017.08.010, ISSN: 1097-4164.

Dungrawala H, Bhat KP, Le Meur R, Chazin WJ, Ding X, Sharan SK, Wessel SR, Sathe AA, Zhao R, Cortez D. RADX Promotes Genome Stability and Modulates Chemosensitivity by Regulating RAD51 at Replication Forks. Mol. Cell [print-electronic]. 2017 Aug 8/3/2017; 67(3): 374-386.e5. PMID: 28735897, PMCID: PMC5548441, PII: S1097-2765(17)30457-4, DOI: 10.1016/j.molcel.2017.06.023, ISSN: 1097-4164.

Puccetti MV, Fischer MA, Arrate MP, Boyd KL, Duszynski RJ, Bétous R, Cortez D, Eischen CM. Defective replication stress response inhibits lymphomagenesis and impairs lymphocyte reconstitution. Oncogene [print-electronic]. 2017 May 5/4/2017; 36(18): 2553-64. PMID: 27797382, PMCID: PMC5562353, PII: onc2016408, DOI: 10.1038/onc.2016.408, ISSN: 1476-5594.

Reynolds JJ, Bicknell LS, Carroll P, Higgs MR, Shaheen R, Murray JE, Papadopoulos DK, Leitch A, Murina O, Tarnauskaite Ž, Wessel SR, Zlatanou A, Vernet A, von Kriegsheim A, Mottram RM, Logan CV, Bye H, Li Y, Brean A, Maddirevula S, Challis RC, Skouloudaki K, Almoisheer A, Alsaif HS, Amar A, Prescott NJ, Bober MB, Duker A, Faqeih E, Seidahmed MZ, Al Tala S, Alswaid A, Ahmed S, Al-Aama JY, Altmüller J, Al Balwi M, Brady AF, Chessa L, Cox H, Fischetto R, Heller R, Henderson BD, Hobson E, Nürnberg P, Percin EF, Peron A, Spaccini L, Quigley AJ, Thakur S, Wise CA, Yoon G, Alnemer M, Tomancak P, Yigit G, Taylor AM, Reijns MA, Simpson MA, Cortez D, Alkuraya FS, Mathew CG, Jackson AP, Stewart GS. Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism. Nat. Genet [print-electronic]. 2017 Apr; 49(4): 537-49. PMID: 28191891, PMCID: PMC5450907, PII: ng.3790, DOI: 10.1038/ng.3790, ISSN: 1546-1718.

Cortez D. Proteomic Analyses of the Eukaryotic Replication Machinery. Meth. Enzymol [print-electronic]. 2017; 591: 33-53. PMID: 28645376, PMCID: PMC5962280, PII: S0076-6879(17)30083-6, DOI: 10.1016/bs.mie.2017.03.002, ISSN: 1557-7988.

Bass Thomas, Luzwick Jessica, Kavanaugh Gina, Carroll Clinton, Dungrawala Huzefa, Glick Gloria, Feldkamp Michael, Putney Reid, Chazin Walter, Cortez David. ETAA1 acts at stalled replication forks to maintain genome integrity. Nature Cell Biology. 2016 Oct 10/10/2016; PMID: 27723720.

Chaudhuri Arnab Ray, Callen Elsa, Ding Xia, Cortez David, Cantor Sharon, Fernandez-Capetillo Oscar, Ge Kai, Jonkers Jos, Rlottenberg Sven, Sharan Shyam, Nussenzweig Andre, et al. Replication fork stability confers chemoresistance in BRCA-deficient cells. Nature. 2016 Jul 7/20/2016; 535: 382-7. PMID: 27443740.

Badu-Nkansah Akosua, Mason Aaron, Eichman Brandt, Cortez David. Identification of a Substrate Recognition Domain in the Replication Stress Response Protein Zinc Finger Ran-binding Domain-containing Protein 3 (ZRANB3). J Biol Chem. 2016 Feb 2/16/2016; 291(15): 8251-7. PMID: 26884333.

Poole LA, Zhao R, Glick GG, Lovejoy CA, Eischen CM, Cortez D. SMARCAL1 maintains telomere integrity during DNA replication. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Dec 12/1/2015; 112(48): 14864-9. PMID: 26578802, PMCID: PMC4672769, PII: 1510750112, DOI: 10.1073/pnas.1510750112, ISSN: 1091-6490.

Cortez D, Zhou Z, Sanchez Y. Stephen Elledge and the DNA damage response. DNA Repair (Amst.). 2015 Nov; 35: 156-7. PMID: 26574138, PII: S1568-7864(15)00216-5, DOI: 10.1016/j.dnarep.2015.11.001, ISSN: 1568-7856.

Kavanaugh G, Ye F, Mohni KN, Luzwick JW, Glick G, Cortez D. A whole genome RNAi screen identifies replication stress response genes. DNA Repair (Amst.) [print-electronic]. 2015 Nov; 35: 55-62. PMID: 26454783, PMCID: PMC4651756, PII: S1568-7864(15)30004-5, DOI: 10.1016/j.dnarep.2015.09.024, ISSN: 1568-7856.

Carroll C, Hunley TE, Guo Y, Cortez D. A novel splice site mutation in SMARCAL1 results in aberrant exon definition in a child with schimke immunoosseous dysplasia. Am. J. Med. Genet. A [print-electronic]. 2015 Oct; 167A(10): 2260-4. PMID: 25943327, DOI: 10.1002/ajmg.a.37146, ISSN: 1552-4833.

Dungrawala H, Rose KL, Bhat KP, Mohni KN, Glick GG, Couch FB, Cortez D. The Replication Checkpoint Prevents Two Types of Fork Collapse without Regulating Replisome Stability. Mol. Cell [print-electronic]. 2015 Sep 9/17/2015; 59(6): 998-1010. PMID: 26365379, PMCID: PMC4575883, PII: S1097-2765(15)00588-2, DOI: 10.1016/j.molcel.2015.07.030, ISSN: 1097-4164.

Kavanaugh G, Zhao R, Guo Y, Mohni KN, Glick G, Lacy ME, Hutson MS, Ascano M, Cortez D. Enhancer of Rudimentary Homolog Affects the Replication Stress Response through Regulation of RNA Processing. Mol. Cell. Biol [print-electronic]. 2015 Sep 9/1/2015; 35(17): 2979-90. PMID: 26100022, PMCID: PMC4525316, PII: MCB.01276-14, DOI: 10.1128/MCB.01276-14, ISSN: 1098-5549.

Cortez D. Preventing replication fork collapse to maintain genome integrity. DNA Repair (Amst.) [print-electronic]. 2015 Aug; 32: 149-57. PMID: 25957489, PMCID: PMC4522347, PII: S1568-7864(15)00118-4, DOI: 10.1016/j.dnarep.2015.04.026, ISSN: 1568-7856.

Bhat KP, Bétous R, Cortez D. High-affinity DNA-binding domains of replication protein A (RPA) direct SMARCAL1-dependent replication fork remodeling. J. Biol. Chem [print-electronic]. 2015 Feb 2/13/2015; 290(7): 4110-7. PMID: 25552480, PMCID: PMC4326820, PII: M114.627083, DOI: 10.1074/jbc.M114.627083, ISSN: 1083-351X.

Mohni KN, Thompson PS, Luzwick JW, Glick GG, Pendleton CS, Lehmann BD, Pietenpol JA, Cortez D. A Synthetic Lethal Screen Identifies DNA Repair Pathways that Sensitize Cancer Cells to Combined ATR Inhibition and Cisplatin Treatments. PLoS ONE. 2015; 10(5): e0125482. PMID: 25965342, PMCID: PMC4428765, PII: PONE-D-14-51553, DOI: 10.1371/journal.pone.0125482, ISSN: 1932-6203.

Ning B, Feldkamp MD, Cortez D, Chazin WJ, Friedman KL, Fanning E. Simian virus Large T antigen interacts with the N-terminal domain of the 70 kD subunit of Replication Protein A in the same mode as multiple DNA damage response factors. PLoS ONE. 2015; 10(2): e0116093. PMID: 25706313, PMCID: PMC4337903, PII: PONE-D-14-45260, DOI: 10.1371/journal.pone.0116093, ISSN: 1932-6203.

Dungrawala H, Cortez D. Purification of proteins on newly synthesized DNA using iPOND. Methods Mol. Biol. 2015; 1228: 123-31. PMID: 25311126, PMCID: PMC4384176, DOI: 10.1007/978-1-4939-1680-1_10, ISSN: 1940-6029.

Sowd GA, Mody D, Eggold J, Cortez D, Friedman KL, Fanning E. SV40 utilizes ATM kinase activity to prevent non-homologous end joining of broken viral DNA replication products. PLoS Pathog. 2014 Dec; 10(12): e1004536. PMID: 25474690, PMCID: PMC4256475, PII: PPATHOGENS-D-14-01844, DOI: 10.1371/journal.ppat.1004536, ISSN: 1553-7374.

Mason AC, Rambo RP, Greer B, Pritchett M, Tainer JA, Cortez D, Eichman BF. A structure-specific nucleic acid-binding domain conserved among DNA repair proteins. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2014 May 5/27/2014; 111(21): 7618-23. PMID: 24821763, PMCID: PMC4040553, PII: 1324143111, DOI: 10.1073/pnas.1324143111, ISSN: 1091-6490.

Mohni KN, Kavanaugh GM, Cortez D. ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency. Cancer Res [print-electronic]. 2014 May 5/15/2014; 74(10): 2835-45. PMID: 24662920, PMCID: PMC4043842, PII: 0008-5472.CAN-13-3229, DOI: 10.1158/0008-5472.CAN-13-3229, ISSN: 1538-7445.

Frank AO, Vangamudi B, Feldkamp MD, Souza-Fagundes EM, Luzwick JW, Cortez D, Olejniczak ET, Waterson AG, Rossanese OW, Chazin WJ, Fesik SW. Discovery of a potent stapled helix peptide that binds to the 70N domain of replication protein A. J. Med. Chem [print-electronic]. 2014 Mar 3/27/2014; 57(6): 2455-61. PMID: 24491171, PMCID: PMC3969094, DOI: 10.1021/jm401730y, ISSN: 1520-4804.

Carroll C, Bansbach CE, Zhao R, Jung SY, Qin J, Cortez D. Phosphorylation of a C-terminal auto-inhibitory domain increases SMARCAL1 activity. Nucleic Acids Res [print-electronic]. 2014 Jan; 42(2): 918-25. PMID: 24150942, PMCID: PMC3902923, PII: gkt929, DOI: 10.1093/nar/gkt929, ISSN: 1362-4962.

Luzwick JW, Nam EA, Zhao R, Cortez D. Mutation of serine 1333 in the ATR HEAT repeats creates a hyperactive kinase. PLoS ONE. 2014; 9(6): e99397. PMID: 24901225, PMCID: PMC4047089, PII: PONE-D-14-10562, DOI: 10.1371/journal.pone.0099397, ISSN: 1932-6203.

Couch FB, Cortez D. Fork reversal, too much of a good thing [editorial]. Cell Cycle [print-electronic]. 2014; 13(7): 1049-50. PMID: 24553113, PMCID: PMC4013150, PII: 28212, DOI: 10.4161/cc.28212, ISSN: 1551-4005.

Carroll C, Badu-Nkansah A, Hunley T, Baradaran-Heravi A, Cortez D, Frangoul H. Schimke Immunoosseous Dysplasia associated with undifferentiated carcinoma and a novel SMARCAL1 mutation in a child. Pediatr Blood Cancer [print-electronic]. 2013 Sep; 60(9): E88-90. PMID: 23630135, PMCID: PMC3713188, DOI: 10.1002/pbc.24542, ISSN: 1545-5017.

Sirbu BM, Cortez D. DNA damage response: three levels of DNA repair regulation. Cold Spring Harb Perspect Biol. 2013 Aug; 5(8): a012724. PMID: 23813586, PMCID: PMC3721278, PII: cshperspect.a012724, DOI: 10.1101/cshperspect.a012724, ISSN: 1943-0264.

Couch FB, Bansbach CE, Driscoll R, Luzwick JW, Glick GG, Bétous R, Carroll CM, Jung SY, Qin J, Cimprich KA, Cortez D. ATR phosphorylates SMARCAL1 to prevent replication fork collapse. Genes Dev. 2013 Jul 7/15/2013; 27(14): 1610-23. PMID: 23873943, PMCID: PMC3731549, PII: 27/14/1610, DOI: 10.1101/gad.214080.113, ISSN: 1549-5477.

Summers AR, Fischer MA, Stengel KR, Zhao Y, Kaiser JF, Wells CE, Hunt A, Bhaskara S, Luzwick JW, Sampathi S, Chen X, Thompson MA, Cortez D, Hiebert SW. HDAC3 is essential for DNA replication in hematopoietic progenitor cells. J. Clin. Invest [print-electronic]. 2013 Jul; 123(7): 3112-23. PMID: 23921131, PMCID: PMC3696547, PII: 60806, DOI: 10.1172/JCI60806, ISSN: 1558-8238.

Bétous R, Couch FB, Mason AC, Eichman BF, Manosas M, Cortez D. Substrate-selective repair and restart of replication forks by DNA translocases. Cell Rep [print-electronic]. 2013 Jun 6/27/2013; 3(6): 1958-69. PMID: 23746452, PMCID: PMC3700663, PII: S2211-1247(13)00219-2, DOI: 10.1016/j.celrep.2013.05.002, ISSN: 2211-1247.

Bétous R, Glick GG, Zhao R, Cortez D. Identification and characterization of SMARCAL1 protein complexes. PLoS ONE [electronic-print]. 2013; 8(5): e63149. PMID: 23671665, PMCID: PMC3650004, PII: PONE-D-13-02305, DOI: 10.1371/journal.pone.0063149, ISSN: 1932-6203.

Wells CE, Bhaskara S, Stengel KR, Zhao Y, Sirbu B, Chagot B, Cortez D, Khabele D, Chazin WJ, Cooper A, Jacques V, Rusche J, Eischen CM, McGirt LY, Hiebert SW. Inhibition of histone deacetylase 3 causes replication stress in cutaneous T cell lymphoma. PLoS ONE [electronic-print]. 2013; 8(7): e68915. PMID: 23894374, PMCID: PMC3718806, PII: PONE-D-13-03947, DOI: 10.1371/journal.pone.0068915, ISSN: 1932-6203.

Sirbu BM, Couch FB, Cortez D. Monitoring the spatiotemporal dynamics of proteins at replication forks and in assembled chromatin using isolation of proteins on nascent DNA. Nat Protoc. 2012 Mar; 7(3): 594-605. PMID: 22383038, PMCID: PMC3671908, PII: nprot.2012.010, DOI: 10.1038/nprot.2012.010, ISSN: 1750-2799.

Bétous R, Mason AC, Rambo RP, Bansbach CE, Badu-Nkansah A, Sirbu BM, Eichman BF, Cortez D. SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication. Genes Dev. 2012 Jan 1/15/2012; 26(2): 151-62. PMID: 22279047, PMCID: PMC3273839, PII: 26/2/151, DOI: 10.1101/gad.178459.111, ISSN: 1549-5477.

Nam EA, Zhao R, Cortez D. Analysis of mutations that dissociate G(2) and essential S phase functions of human ataxia telangiectasia-mutated and Rad3-related (ATR) protein kinase. J. Biol. Chem [print-electronic]. 2011 Oct 10/28/2011; 286(43): 37320-7. PMID: 21908846, PMCID: PMC3199479, PII: M111.276113, DOI: 10.1074/jbc.M111.276113, ISSN: 1083-351X.

Nam EA, Zhao R, Glick GG, Bansbach CE, Friedman DB, Cortez D. Thr-1989 phosphorylation is a marker of active ataxia telangiectasia-mutated and Rad3-related (ATR) kinase. J. Biol. Chem [print-electronic]. 2011 Aug 8/19/2011; 286(33): 28707-14. PMID: 21705319, PMCID: PMC3190678, PII: M111.248914, DOI: 10.1074/jbc.M111.248914, ISSN: 1083-351X.

Bansbach CE, Cortez D. Defining genome maintenance pathways using functional genomic approaches. Crit. Rev. Biochem. Mol. Biol. 2011 Aug; 46(4): 327-41. PMID: 21787120, PMCID: PMC3144553, DOI: 10.3109/10409238.2011.588938, ISSN: 1549-7798.

Sirbu BM, Couch FB, Feigerle JT, Bhaskara S, Hiebert SW, Cortez D. Analysis of protein dynamics at active, stalled, and collapsed replication forks. Genes Dev. 2011 Jun 6/15/2011; 25(12): 1320-7. PMID: 21685366, PMCID: PMC3127432, PII: 25/12/1320, DOI: 10.1101/gad.2053211, ISSN: 1549-5477.

Nam EA, Cortez D. ATR signalling: more than meeting at the fork. Biochem. J. 2011 Jun 6/15/2011; 436(3): 527-36. PMID: 21615334, PMCID: PMC3678388, PII: BJ20102162, DOI: 10.1042/BJ20102162, ISSN: 1470-8728.

Yu DS, Zhao R, Hsu EL, Cayer J, Ye F, Guo Y, Shyr Y, Cortez D. Cyclin-dependent kinase 9-cyclin K functions in the replication stress response. EMBO Rep [print-electronic]. 2010 Nov; 11(11): 876-82. PMID: 20930849, PMCID: PMC2966956, PII: embor2010153, DOI: 10.1038/embor.2010.153, ISSN: 1469-3178.

Wiltshire TD, Lovejoy CA, Wang T, Xia F, O'Connor MJ, Cortez D. Sensitivity to poly(ADP-ribose) polymerase (PARP) inhibition identifies ubiquitin-specific peptidase 11 (USP11) as a regulator of DNA double-strand break repair. J. Biol. Chem [print-electronic]. 2010 May 5/7/2010; 285(19): 14565-71. PMID: 20233726, PMCID: PMC2863164, PII: M110.104745, DOI: 10.1074/jbc.M110.104745, ISSN: 1083-351X.

Bansbach CE, Boerkoel CF, Cortez D. SMARCAL1 and replication stress: an explanation for SIOD?. Nucleus [print-electronic]. 2010 May; 1(3): 245-8. PMID: 21327070, PMCID: PMC3027029, DOI: 10.4161/nucl.1.3.11739, ISSN: 1949-1042.

Lovejoy CA, Xu X, Bansbach CE, Glick GG, Zhao R, Ye F, Sirbu BM, Titus LC, Shyr Y, Cortez D. Functional genomic screens identify CINP as a genome maintenance protein. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2009 Nov 11/17/2009; 106(46): 19304-9. PMID: 19889979, PMCID: PMC2780779, PII: 0909345106, DOI: 10.1073/pnas.0909345106, ISSN: 1091-6490.

Bansbach CE, Bétous R, Lovejoy CA, Glick GG, Cortez D. The annealing helicase SMARCAL1 maintains genome integrity at stalled replication forks. Genes Dev [print-electronic]. 2009 Oct 10/15/2009; 23(20): 2405-14. PMID: 19793861, PMCID: PMC2764496, PII: gad.1839909, DOI: 10.1101/gad.1839909, ISSN: 1549-5477.

Lovejoy CA, Cortez D. Common mechanisms of PIKK regulation. DNA Repair (Amst.) [print-electronic]. 2009 Sep 9/2/2009; 8(9): 1004-8. PMID: 19464237, PMCID: PMC2725225, PII: S1568-7864(09)00100-1, DOI: 10.1016/j.dnarep.2009.04.006, ISSN: 1568-7856.

Nam EA, Cortez D. SOSS1/2: Sensors of single-stranded DNA at a break. Mol. Cell. 2009 Aug 8/14/2009; 35(3): 258-9. PMID: 19683490, PMCID: PMC2820400, PII: S1097-2765(09)00512-7, DOI: 10.1016/j.molcel.2009.07.016, ISSN: 1097-4164.

Mordes DA, Nam EA, Cortez D. Dpb11 activates the Mec1-Ddc2 complex. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2008 Dec 12/2/2008; 105(48): 18730-4. PMID: 19028869, PMCID: PMC2596233, PII: 0806621105, DOI: 10.1073/pnas.0806621105, ISSN: 1091-6490.

Xu X, Vaithiyalingam S, Glick GG, Mordes DA, Chazin WJ, Cortez D. The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling. Mol. Cell. Biol [print-electronic]. 2008 Dec; 28(24): 7345-53. PMID: 18936170, PMCID: PMC2593429, PII: MCB.01079-08, DOI: 10.1128/MCB.01079-08, ISSN: 1098-5549.

Mordes DA, Cortez D. Activation of ATR and related PIKKs. Cell Cycle [print-electronic]. 2008 Sep 9/15/2008; 7(18): 2809-12. PMID: 18769153, PMCID: PMC2672405, PII: 6689, ISSN: 1551-4005.

Cimprich KA, Cortez D. ATR: an essential regulator of genome integrity. Nat. Rev. Mol. Cell Biol [print-electronic]. 2008 Aug; 9(8): 616-27. PMID: 18594563, PMCID: PMC2663384, PII: nrm2450, DOI: 10.1038/nrm2450, ISSN: 1471-0080.

Mordes DA, Glick GG, Zhao R, Cortez D. TopBP1 activates ATR through ATRIP and a PIKK regulatory domain. Genes Dev. 2008 Jun 6/1/2008; 22(11): 1478-89. PMID: 18519640, PMCID: PMC2418584, PII: 22/11/1478, DOI: 10.1101/gad.1666208, ISSN: 0890-9369.

Bhaskara S, Chyla BJ, Amann JM, Knutson SK, Cortez D, Sun ZW, Hiebert SW. Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control. Mol. Cell. 2008 Apr 4/11/2008; 30(1): 61-72. PMID: 18406327, PMCID: PMC2373760, PII: S1097-2765(08)00207-4, DOI: 10.1016/j.molcel.2008.02.030, ISSN: 1097-4164.

Myers JS, Zhao R, Xu X, Ham AJ, Cortez D. Cyclin-dependent kinase 2 dependent phosphorylation of ATRIP regulates the G2-M checkpoint response to DNA damage. Cancer Res. 2007 Jul 7/15/2007; 67(14): 6685-90. PMID: 17638878, PMCID: PMC2728292, PII: 67/14/6685, DOI: 10.1158/0008-5472.CAN-07-0495, ISSN: 0008-5472.

Chen X, Zhao R, Glick GG, Cortez D. Function of the ATR N-terminal domain revealed by an ATM/ATR chimera. Exp. Cell Res [print-electronic]. 2007 May 5/1/2007; 313(8): 1667-74. PMID: 17376433, PMCID: PMC1855264, PII: S0014-4827(07)00084-5, DOI: 10.1016/j.yexcr.2007.02.015, ISSN: 0014-4827.

Ball HL, Ehrhardt MR, Mordes DA, Glick GG, Chazin WJ, Cortez D. Function of a conserved checkpoint recruitment domain in ATRIP proteins. Mol. Cell. Biol [print-electronic]. 2007 May; 27(9): 3367-77. PMID: 17339343, PMCID: PMC1899971, PII: MCB.02238-06, DOI: 10.1128/MCB.02238-06, ISSN: 0270-7306.

Lovejoy CA, Lock K, Yenamandra A, Cortez D. DDB1 maintains genome integrity through regulation of Cdt1. Mol. Cell. Biol [print-electronic]. 2006 Nov; 26(21): 7977-90. PMID: 16940174, PMCID: PMC1636754, PII: MCB.00819-06, DOI: 10.1128/MCB.00819-06, ISSN: 0270-7306.

Myers JS, Cortez D. Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J. Biol. Chem [print-electronic]. 2006 Apr 4/7/2006; 281(14): 9346-50. PMID: 16431910, PMCID: PMC1821075, PII: M513265200, DOI: 10.1074/jbc.M513265200, ISSN: 0021-9258.

Ball HL, Cortez D. ATRIP oligomerization is required for ATR-dependent checkpoint signaling. J. Biol. Chem [print-electronic]. 2005 Sep 9/9/2005; 280(36): 31390-6. PMID: 16027118, PMCID: PMC1360181, PII: M504961200, DOI: 10.1074/jbc.M504961200, ISSN: 0021-9258.

Ball HL, Myers JS, Cortez D. ATRIP binding to replication protein A-single-stranded DNA promotes ATR-ATRIP localization but is dispensable for Chk1 phosphorylation. Mol. Biol. Cell [print-electronic]. 2005 May; 16(5): 2372-81. PMID: 15743907, PMCID: PMC1087242, PII: E04-11-1006, DOI: 10.1091/mbc.E04-11-1006, ISSN: 1059-1524.

Cortez D. Unwind and slow down: checkpoint activation by helicase and polymerase uncoupling. Genes Dev. 2005 May 5/1/2005; 19(9): 1007-12. PMID: 15879550, PMCID: PMC1360198, PII: 19/9/1007, DOI: 10.1101/gad.1316905, ISSN: 0890-9369.

Cortez D, Glick G, Elledge SJ. Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2004 Jul 7/6/2004; 101(27): 10078-83. PMID: 15210935, PMCID: PMC454167, PII: 0403410101, DOI: 10.1073/pnas.0403410101, ISSN: 0027-8424.

Cortez D. Caffeine inhibits checkpoint responses without inhibiting the ataxia-telangiectasia-mutated (ATM) and ATM- and Rad3-related (ATR) protein kinases. J. Biol. Chem [print-electronic]. 2003 Sep 9/26/2003; 278(39): 37139-45. PMID: 12847089, PII: M307088200, DOI: 10.1074/jbc.M307088200, ISSN: 0021-9258.

Zou L, Cortez D, Elledge SJ. Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatin. Genes Dev. 2002 Jan 1/15/2002; 16(2): 198-208. PMID: 11799063, PMCID: PMC155323, DOI: 10.1101/gad.950302, ISSN: 0890-9369.

Cortez D, Guntuku S, Qin J, Elledge SJ. ATR and ATRIP: partners in checkpoint signaling. Science. 2001 Nov 11/23/2001; 294(5547): 1713-6. PMID: 11721054, PII: 294/5547/1713, DOI: 10.1126/science.1065521, ISSN: 0036-8075.

Paull T.T., Cortez D., Bowers B., Elledge S.J., Gellert M. Direct DNA binding by Brca1. Proc. Natl. Acad. Sci. USA. 2001; 98: 6086-91.

Liu Q, Guntuku S, Cui XS, Matsuoka S, Cortez D, Tamai K, Luo G, Carattini-Rivera S, DeMayo F, Bradley A, Donehower LA, Elledge SJ. Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint. Genes Dev. 2000 Jun 6/15/2000; 14(12): 1448-59. PMID: 10859164, PMCID: PMC316686, ISSN: 0890-9369.

Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 2000 Apr 4/15/2000; 14(8): 927-39. PMID: 10783165, PMCID: PMC316544, ISSN: 0890-9369.

Tibbetts, R.S., Cortez, D., Brumbaugh, K.M., Scully, R., Livingston, D., Elledge, S.J., Abraham, R.T. Functional interactions betweeen BRCA1 and the checkpoint kinase ATR during genotoxic stress. Genes and Development. 2000; 14: 2989-3002.

Cortez D, Elledge SJ. Conducting the mitotic symphony. Nature. 2000; 406: 354-6.

Cortez D, Wang Y, Qin J, Elledge SJ. Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science. 1999 Nov 11/5/1999; 286(5442): 1162-6. PMID: 10550055, PII: 7959, ISSN: 0036-8075.

Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ. The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes. Curr. Biol. 1998 Dec 12/3/1998; 8(24): 1300-9. PMID: 9843682, PII: S0960-9822(07)00560-X, ISSN: 0960-9822.

Dai, Z., Quackenbush, R.C., Courtney, K.D. Grove, M., Cortez, D., Reuther, G.W., Pendergast, A.M. Oncogenic Abl and Src tyrosine kinases elicit the ubiquitin-dependent degradation of target proteins through a Ras-independent pathway. Genes and Development. 1998; 12: 1415-24.

Reuther, J., Reuther, G., Cortez, D., Pendergast, A.M., Baldwin, S.A. Activation of NF-kB is required for Bcr-Abl-mediated tumorigenesis. Genes and Development. 1998; 12: 968-81.

Cortez, D., Reuther, G., and Pendergast, A.M. The Bcr-Abl tyrosine kinase activates mitogenic signaling pathways and stimulates G1-to-S phase transition in hematopoietic cells. Oncogene. 1997; 15: 2333-42.

Cortez, D., Stoica, G., Pierce, J.H., Pendergast, A.M. The BCR-ABL tyrosine kinase inhibits apoptosis by activating a Ras-dependent signaling pathway. Oncogene. 1996; 13: 2589-94.

Ghizisky, M.L., Cortez, D., Pendergast, A.M. Grb2 mutant proteins reverse BCR-ABL-induced transformation. Proc. Natl. Acad. Sci. USA. 1995; 92: 10889-93.

Cortez, D., Kadlec, L., Pendergast, A.M. Structural and signaling requirements for BCR-ABL-mediated transformation and inhibition of apoptosis. Mol. Cell. Biol. 1995; 15: 5531-41.

Seo KY, Riley J, Cortez D, Wagner ED, Plewa MJ. Characterization of stable high molecular weight mutagenic product(s) of plant-activated m-phenylenediamine. Mutat. Res. 1993 Apr; 299(2): 111-20. PMID: 7680425, PII: 0165-1218(93)90088-U, ISSN: 0027-5107.

Available Postdoctoral Position Details
Posted: 8/6/2013
Postdoctoral positions are available in the areas of molecular cancer biology, signal transduction, cell cycle, and DNA repair. Please submit a CV and three letters of recommendation.