Charles E. Cobb, Ph.D.

Faculty Appointments
Professor of Molecular Physiology & Biophysics
Ph.D., Physiology, Vanderbilt University, Nashville, TennesseeM.S., Biochemistry, Michigan Technological University , Houghton, MichiganB.S., Biochemistry, Michigan Technological University , Houghton, Michigan
Office Address
735 A Light Hall
Nashville, TN 0615
Research Description
Research Keywords
Membrane Protein Structure and Function, Antioxidant Vitamins, ESR Spectroscopy, Fluorescence Spectroscopy
Edwards SJ, Moth CW, Kim S, Brandon S, Zhou Z, Cobb CE, Hustedt EJ, Beth AH, Smith JA, Lybrand TP. Automated structure refinement for a protein heterodimer complex using limited EPR spectroscopic data and a rigid-body docking algorithm: a three-dimensional model for an ankyrin-CDB3 complex. J Phys Chem B [print-electronic]. 2014 May 5/8/2014; 118(18): 4717-26. PMID: 24758720, PMCID: PMC4018176, DOI: 10.1021/jp4099705, ISSN: 1520-5207.

Dixit M, Kim S, Matthews GF, Erreger K, Galli A, Cobb CE, Hustedt EJ, Beth AH. Structural arrangement of the intracellular Ca2+ binding domains of the cardiac Na+/Ca2+ exchanger (NCX1.1): effects of Ca2+ binding. J. Biol. Chem [print-electronic]. 2013 Feb 2/8/2013; 288(6): 4194-207. PMID: 23233681, PMCID: PMC3567668, PII: M112.423293, DOI: 10.1074/jbc.M112.423293, ISSN: 1083-351X.

Kim S, Brandon S, Zhou Z, Cobb CE, Edwards SJ, Moth CW, Parry CS, Smith JA, Lybrand TP, Hustedt EJ, Beth AH. Determination of structural models of the complex between the cytoplasmic domain of erythrocyte band 3 and ankyrin-R repeats 13-24. J. Biol. Chem [print-electronic]. 2011 Jun 6/10/2011; 286(23): 20746-57. PMID: 21493712, PMCID: PMC3121456, PII: M111.230326, DOI: 10.1074/jbc.M111.230326, ISSN: 1083-351X.

Zhou Z, DeSensi SC, Stein RA, Brandon S, Song L, Cobb CE, Hustedt EJ, Beth AH. Structure of the cytoplasmic domain of erythrocyte band 3 hereditary spherocytosis variant P327R: band 3 Tuscaloosa. Biochemistry [print-electronic]. 2007 Sep 9/11/2007; 46(36): 10248-57. PMID: 17696498, DOI: 10.1021/bi700948p, ISSN: 0006-2960.

May JM, Li L, Qu ZC, Cobb CE. Mitochondrial recycling of ascorbic acid as a mechanism for regenerating cellular ascorbate. Biofactors. 2007; 30(1): 35-48. PMID: 18198400, ISSN: 0951-6433.

Zhou Z, DeSensi SC, Stein RA, Brandon S, Dixit M, McArdle EJ, Warren EM, Kroh HK, Song L, Cobb CE, Hustedt EJ, Beth AH. Solution structure of the cytoplasmic domain of erythrocyte membrane band 3 determined by site-directed spin labeling. Biochemistry. 2005 Nov 11/22/2005; 44(46): 15115-28. PMID: 16285715, DOI: 10.1021/bi050931t, ISSN: 0006-2960.

May JM, Qu ZC, Juliao S, Cobb CE. Ascorbic acid decreases oxidant stress in endothelial cells caused by the nitroxide tempol. Free Radic. Res. 2005 Feb; 39(2): 195-202. PMID: 15763967, PII: JKQ36HM3D3NPQ9U0, DOI: 10.1080/10715760400019661, ISSN: 1071-5762.

May JM, Qu ZC, Cobb CE. Reduction and uptake of methylene blue by human erythrocytes. Am. J. Physiol., Cell Physiol [print-electronic]. 2004 Jun; 286(6): C1390-8. PMID: 14973146, PII: 00512.2003, DOI: 10.1152/ajpcell.00512.2003, ISSN: 0363-6143.

May JM, Qu ZC, Cobb CE. Human erythrocyte recycling of ascorbic acid: relative contributions from the ascorbate free radical and dehydroascorbic acid. J. Biol. Chem [print-electronic]. 2004 Apr 4/9/2004; 279(15): 14975-82. PMID: 14752116, PII: M312548200, DOI: 10.1074/jbc.M312548200, ISSN: 0021-9258.

Li X, Cobb CE, May JM. Mitochondrial recycling of ascorbic acid from dehydroascorbic acid: dependence on the electron transport chain. Arch. Biochem. Biophys. 2002 Jul 7/1/2002; 403(1): 103-10. PMID: 12061807, PII: S0003-9861(02)00205-9, DOI: 10.1016/S0003-9861(02)00205-9, ISSN: 0003-9861.

Blackman SM, Hustedt EJ, Cobb CE, Beth AH. Flexibility of the cytoplasmic domain of the anion exchange protein, band 3, in human erythrocytes. Biophys. J. 2001 Dec; 81(6): 3363-76. PMID: 11720999, PMCID: PMC1301793, PII: S0006-3495(01)75969-3, DOI: 10.1016/S0006-3495(01)75969-3, ISSN: 0006-3495.

May JM, Qu Z, Cobb CE. Recycling of the ascorbate free radical by human erythrocyte membranes. Free Radic. Biol. Med. 2001 Jul 7/1/2001; 31(1): 117-24. PMID: 11425497, PII: S0891584901005664, ISSN: 0891-5849.

Li X, Cobb CE, Hill KE, Burk RF, May JM. Mitochondrial uptake and recycling of ascorbic acid. Arch. Biochem. Biophys. 2001 Mar 3/1/2001; 387(1): 143-53. PMID: 11368176, PII: S0003-9861(00)92245-8, DOI: 10.1006/abbi.2000.2245, ISSN: 0003-9861.

Blackman, S.M, Hustedt, E.J., Cobb, C.E., and Beth, A.H. Flexibility of the cytoplasmic domain of the anion exchange protein, band 3, in human erythrocytes. 2001.

May JM, Qu ZC, Xia L, Cobb CE. Nitrite uptake and metabolism and oxidant stress in human erythrocytes. Am. J. Physiol., Cell Physiol. 2000 Dec; 279(6): C1946-54. PMID: 11078710, ISSN: 0363-6143.

May JM, Qu Z, Morrow JD, Cobb CE. Ascorbate-dependent protection of human erythrocytes against oxidant stress generated by extracellular diazobenzene sulfonate. Biochem. Pharmacol. 2000 Jul 7/1/2000; 60(1): 47-53. PMID: 10807944, PII: S0006295200003129, ISSN: 0006-2952.

May JM, Qu Zc, Cobb CE. Extracellular reduction of the ascorbate free radical by human erythrocytes. Biochem. Biophys. Res. Commun. 2000 Jan 1/7/2000; 267(1): 118-23. PMID: 10623584, PII: S0006291X9991906X, DOI: 10.1006/bbrc.1999.1906, ISSN: 0006-291X.

Ross D, Mendiratta S, Qu ZC, Cobb CE, May JM. Ascorbate 6-palmitate protects human erythrocytes from oxidative damage. Free Radic. Biol. Med. 1999 Jan; 26(1-2): 81-9. PMID: 9890643, PII: S0891-5849(98)00198-1, ISSN: 0891-5849.

May JM, Cobb CE, Mendiratta S, Hill KE, Burk RF. Reduction of the ascorbyl free radical to ascorbate by thioredoxin reductase. J. Biol. Chem. 1998 Sep 9/4/1998; 273(36): 23039-45. PMID: 9722529, ISSN: 0021-9258.

Hustedt EJ, Smirnov AI, Laub CF, Cobb CE, Beth AH. Molecular distances from dipolar coupled spin-labels: the global analysis of multifrequency continuous wave electron paramagnetic resonance data. Biophys. J. 1997 Apr; 72(4): 1861-77. PMID: 9083690, PMCID: PMC1184380, PII: S0006-3495(97)78832-5, DOI: 10.1016/S0006-3495(97)78832-5, ISSN: 0006-3495.

Blackman SM, Cobb CE, Beth AH, Piston DW. The orientation of eosin-5-maleimide on human erythrocyte band 3 measured by fluorescence polarization microscopy. Biophys. J. 1996 Jul; 71(1): 194-208. PMID: 8804603, PMCID: PMC1233471, PII: S0006-3495(96)79216-0, DOI: 10.1016/S0006-3495(96)79216-0, ISSN: 0006-3495.

Scothorn DJ, Wojcicki WE, Hustedt EJ, Beth AH, Cobb CE. Synthesis and characterization of a novel spin-labeled affinity probe of human erythrocyte band 3: characteristics of the stilbenedisulfonate binding site. Biochemistry. 1996 May 5/28/1996; 35(21): 6931-43. PMID: 8639645, PII: bi960150f, DOI: 10.1021/bi960150f, ISSN: 0006-2960.

May JM, Qu ZC, Whitesell RR, Cobb CE. Ascorbate recycling in human erythrocytes: role of GSH in reducing dehydroascorbate. Free Radic. Biol. Med. 1996; 20(4): 543-51. PMID: 8904295, PII: 0891584995021302, ISSN: 0891-5849.

May JM, Qu ZC, Cobb CE. Accessibility and reactivity of ascorbate 6-palmitate bound to erythrocyte membranes. Free Radic. Biol. Med. 1996; 21(4): 471-80. PMID: 8886797, PII: 0891584996001232, ISSN: 0891-5849.

Hustedt EJ, Cobb CE, Beth AH, Beechem JM. Measurement of rotational dynamics by the simultaneous nonlinear analysis of optical and EPR data. Biophys. J. 1993 Mar; 64(3): 614-21. PMID: 7682452, PMCID: PMC1262373, PII: S0006-3495(93)81420-6, DOI: 10.1016/S0006-3495(93)81420-6, ISSN: 0006-3495.

Cobb CE, Hustedt EJ, Beechem JM, Beth AH. Protein rotational dynamics investigated with a dual EPR/optical molecular probe. Spin-labeled eosin. Biophys. J. 1993 Mar; 64(3): 605-13. PMID: 7682451, PMCID: PMC1262372, PII: S0006-3495(93)81419-X, DOI: 10.1016/S0006-3495(93)81419-X, ISSN: 0006-3495.

Cobb CE, Juliao S, Balasubramanian K, Staros JV, Beth AH. Effects of diethyl ether on membrane lipid ordering and on rotational dynamics of the anion exchange protein in intact human erythrocytes: correlations with anion exchange function. Biochemistry. 1990 Dec 12/4/1990; 29(48): 10799-806. PMID: 2176884, ISSN: 0006-2960.

Cobb CE, Beth AH. Identification of the eosinyl-5-maleimide reaction site on the human erythrocyte anion-exchange protein: overlap with the reaction sites of other chemical probes. Biochemistry. 1990 Sep 9/11/1990; 29(36): 8283-90. PMID: 1701324, ISSN: 0006-2960.

Anjaneyulu PS, Beth AH, Cobb CE, Juliao SF, Sweetman BJ, Staros JV. Bis(sulfo-N-succinimidyl) doxyl-2-spiro-5'-azelate: synthesis, characterization, and reaction with the anion-exchange channel in intact human erythrocytes. Biochemistry. 1989 Aug 8/8/1989; 28(16): 6583-90. PMID: 2477061, ISSN: 0006-2960.

Corbin JD, Cobb CE, Beebe SJ, Granner DK, Koch SR, Gettys TW, Blackmore PF, Francis SH, Wells JN. Mechanism and function of cAMP- and cGMP-dependent protein kinases. Adv. Second Messenger Phosphoprotein Res. 1988; 21: 75-86. PMID: 3137961, ISSN: 1040-7952.

Cobb CE, Beth AH, Corbin JD. Purification and characterization of an inactive form of cAMP-dependent protein kinase containing bound cAMP. J. Biol. Chem. 1987 Dec 12/5/1987; 262(34): 16566-74. PMID: 2824499, ISSN: 0021-9258.