Joel M. Harp, Ph.D.

Research Assistant Professor

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Faculty Appointments
Research Assistant Professor of Biochemistry
Ph.D., Biomedical Sciences, University of Tennessee, Knoxville, TennesseeM.S., Biology, West Texas A&M University , Canyon, TexasB.A., Biology, West Texas A&M University , Canyon, Texas
Office Address
Suite 5140 BioSci/ MRB III
465 21st Avenue South
Nashville, TN 37232-8725
Morin A, Kaufmann KW, Fortenberry C, Harp JM, Mizoue LS, Meiler J. Computational design of an endo-1,4-beta-xylanase ligand binding site. Protein Eng. Des. Sel [print-electronic]. 2011 Jun; 24(6): 503-16. PMID: 21349882, PMCID: PMC3092692, PII: gzr006, DOI: 10.1093/protein/gzr006, ISSN: 1741-0134.

Pallan PS, Greene EM, Jicman PA, Pandey RK, Manoharan M, Rozners E, Egli M. Unexpected origins of the enhanced pairing affinity of 2'-fluoro-modified RNA. Nucleic Acids Res [print-electronic]. 2011 Apr; 39(8): 3482-95. PMID: 21183463, PMCID: PMC3082899, PII: gkq1270, DOI: 10.1093/nar/gkq1270, ISSN: 1362-4962.

Manoharan M, Akinc A, Pandey RK, Qin J, Hadwiger P, John M, Mills K, Charisse K, Maier MA, Nechev L, Greene EM, Pallan PS, Rozners E, Rajeev KG, Egli M. Unique gene-silencing and structural properties of 2'-fluoro-modified siRNAs. Angew. Chem. Int. Ed. Engl [print-electronic]. 2011 Mar 3/1/2011; 50(10): 2284-8. PMID: 21351337, PMCID: PMC3516925, DOI: 10.1002/anie.201006519, ISSN: 1521-3773.

Stourman NV, Branch MC, Schaab MR, Harp JM, Ladner JE, Armstrong RN. Structure and function of YghU, a nu-class glutathione transferase related to YfcG from Escherichia coli. Biochemistry [print-electronic]. 2011 Feb 2/22/2011; 50(7): 1274-81. PMID: 21222452, PMCID: PMC3040281, DOI: 10.1021/bi101861a, ISSN: 1520-4995.

Duggan KC, Walters MJ, Musee J, Harp JM, Kiefer JR, Oates JA, Marnett LJ. Molecular basis for cyclooxygenase inhibition by the non-steroidal anti-inflammatory drug naproxen. J. Biol. Chem [print-electronic]. 2010 Nov 11/5/2010; 285(45): 34950-9. PMID: 20810665, PMCID: PMC2966109, PII: M110.162982, DOI: 10.1074/jbc.M110.162982, ISSN: 1083-351X.

Seth PP, Allerson CR, Berdeja A, Siwkowski A, Pallan PS, Gaus H, Prakash TP, Watt AT, Egli M, Swayze EE. An exocyclic methylene group acts as a bioisostere of the 2'-oxygen atom in LNA. J. Am. Chem. Soc. 2010 Oct 10/27/2010; 132(42): 14942-50. PMID: 20886816, PMCID: PMC2993159, DOI: 10.1021/ja105875e, ISSN: 1520-5126.

Egli M, Pallan PS. The many twists and turns of DNA: template, telomere, tool, and target. Curr. Opin. Struct. Biol [print-electronic]. 2010 Jun; 20(3): 262-75. PMID: 20381338, PMCID: PMC2911523, PII: S0959-440X(10)00039-4, DOI: 10.1016/, ISSN: 1879-033X.

Lepesheva GI, Park HW, Hargrove TY, Vanhollebeke B, Wawrzak Z, Harp JM, Sundaramoorthy M, Nes WD, Pays E, Chaudhuri M, Villalta F, Waterman MR. Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections. J. Biol. Chem [print-electronic]. 2010 Jan 1/15/2010; 285(3): 1773-80. PMID: 19923211, PMCID: PMC2804335, PII: M109.067470, DOI: 10.1074/jbc.M109.067470, ISSN: 1083-351X.

Egli M, Pallan PS. Crystallographic studies of chemically modified nucleic acids: a backward glance. Chem. Biodivers. 2010 Jan; 7(1): 60-89. PMID: 20087997, PMCID: PMC2905155, DOI: 10.1002/cbdv.200900177, ISSN: 1612-1880.

Kolarovic A, Schweizer E, Greene E, Gironda M, Pallan PS, Egli M, Rozners E. Interplay of structure, hydration and thermal stability in formacetal modified oligonucleotides: RNA may tolerate nonionic modifications better than DNA. J. Am. Chem. Soc. 2009 Oct 10/21/2009; 131(41): 14932-7. PMID: 19824732, PMCID: PMC2823299, DOI: 10.1021/ja904926e, ISSN: 1520-5126.

Pallan PS, Egli M. Pairing geometry of the hydrophobic thymine analogue 2,4-difluorotoluene in duplex DNA as analyzed by X-ray crystallography. J. Am. Chem. Soc. 2009 Sep 9/9/2009; 131(35): 12548-9. PMID: 19685868, PMCID: PMC2757930, DOI: 10.1021/ja905739j, ISSN: 1520-5126.

Wadington MC, Ladner JE, Stourman NV, Harp JM, Armstrong RN. Analysis of the structure and function of YfcG from Escherichia coli reveals an efficient and unique disulfide bond reductase. Biochemistry. 2009 Jul 7/21/2009; 48(28): 6559-61. PMID: 19537707, PMCID: PMC2996727, DOI: 10.1021/bi9008825, ISSN: 1520-4995.

Pallan PS, Prakash TP, Li F, Eoff RL, Manoharan M, Egli M. A conformational transition in the structure of a 2'-thiomethyl-modified DNA visualized at high resolution. Chem. Commun. (Camb.) [print-electronic]. 2009 Apr 4/21/2009; (15): 2017-9. PMID: 19333476, PMCID: PMC2909737, DOI: 10.1039/b822781k, ISSN: 1359-7345.

Pallan PS, Kreutz C, Bosio S, Micura R, Egli M. Effects of N2,N2-dimethylguanosine on RNA structure and stability: crystal structure of an RNA duplex with tandem m2 2G:A pairs. RNA [print-electronic]. 2008 Oct; 14(10): 2125-35. PMID: 18772248, PMCID: PMC2553729, PII: rna.1078508, DOI: 10.1261/rna.1078508, ISSN: 1469-9001.

Pallan PS, Egli M. Insights into RNA/DNA hybrid recognition and processing by RNase H from the crystal structure of a non-specific enzyme-dsDNA complex. Cell Cycle [print-electronic]. 2008 Aug 8/15/2008; 7(16): 2562-9. PMID: 18719385, PMCID: PMC3689302, PII: 6461, ISSN: 1551-4005.

Pallan PS, Ittig D, Héroux A, Wawrzak Z, Leumann CJ, Egli M. Crystal structure of tricyclo-DNA: an unusual compensatory change of two adjacent backbone torsion angles. Chem. Commun. (Camb.) [print-electronic]. 2008 Feb 2/21/2008; (7): 883-5. PMID: 18253536, DOI: 10.1039/b716390h, ISSN: 1359-7345.

Leuba SH, Anand SP, Harp JM, Khan SA. Expedient placement of two fluorescent dyes for investigating dynamic DNA protein interactions in real time. Chromosome Res. 2008; 16(3): 451-67. PMID: 18461484, PMCID: PMC2413326, DOI: 10.1007/s10577-008-1235-5, ISSN: 0967-3849.

Irimia A, Eoff RL, Pallan PS, Guengerich FP, Egli M. Structure and activity of Y-class DNA polymerase DPO4 from Sulfolobus solfataricus with templates containing the hydrophobic thymine analog 2,4-difluorotoluene. J. Biol. Chem [print-electronic]. 2007 Dec 12/14/2007; 282(50): 36421-33. PMID: 17951245, PII: M707267200, DOI: 10.1074/jbc.M707267200, ISSN: 0021-9258.

Pallan PS, Lubini P, Egli M. A left-handed supramolecular assembly around a right-handed screw axis in the crystal structure of homo-DNA. Chem. Commun. (Camb.) [print-electronic]. 2007 Apr 4/14/2007; (14): 1447-9. PMID: 17389988, DOI: 10.1039/b614983a, ISSN: 1359-7345.

Pallan PS, Lubini P, Bolli M, Egli M. Backbone-base inclination as a fundamental determinant of nucleic acid self- and cross-pairing. Nucleic Acids Res [print-electronic]. 2007; 35(19): 6611-24. PMID: 17905816, PMCID: PMC2095819, PII: gkm612, DOI: 10.1093/nar/gkm612, ISSN: 1362-4962.

Li F, Pallan PS, Maier MA, Rajeev KG, Mathieu SL, Kreutz C, Fan Y, Sanghvi J, Micura R, Rozners E, Manoharan M, Egli M. Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme. Nucleic Acids Res [print-electronic]. 2007; 35(19): 6424-38. PMID: 17881374, PMCID: PMC2095806, PII: gkm664, DOI: 10.1093/nar/gkm664, ISSN: 1362-4962.

Pallan PS, Egli M. Selenium modification of nucleic acids: preparation of oligonucleotides with incorporated 2'-SeMe-uridine for crystallographic phasing of nucleic acid structures. Nat Protoc. 2007; 2(3): 647-51. PMID: 17406626, PII: nprot.2007.75, DOI: 10.1038/nprot.2007.75, ISSN: 1750-2799.

Pallan PS, Egli M. Selenium modification of nucleic acids: preparation of phosphoroselenoate derivatives for crystallographic phasing of nucleic acid structures. Nat Protoc. 2007; 2(3): 640-6. PMID: 17406625, PII: nprot.2007.74, DOI: 10.1038/nprot.2007.74, ISSN: 1750-2799.

Egli M, Pallan PS. Insights from crystallographic studies into the structural and pairing properties of nucleic acid analogs and chemically modified DNA and RNA oligonucleotides. Annu Rev Biophys Biomol Struct. 2007; 36: 281-305. PMID: 17288535, DOI: 10.1146/annurev.biophys.36.040306.132556, ISSN: 1056-8700.

Egli M, Lubini P, Pallan PS. The long and winding road to the structure of homo-DNA. Chem Soc Rev [print-electronic]. 2007 Jan; 36(1): 31-45. PMID: 17173143, DOI: 10.1039/b606807c, ISSN: 0306-0012.

Egli M, Pallan PS, Pattanayek R, Wilds CJ, Lubini P, Minasov G, Dobler M, Leumann CJ, Eschenmoser A. Crystal structure of homo-DNA and nature's choice of pentose over hexose in the genetic system. J. Am. Chem. Soc. 2006 Aug 8/23/2006; 128(33): 10847-56. PMID: 16910680, DOI: 10.1021/ja062548x, ISSN: 0002-7863.

Pallan, P. D., Marshall, W. S., Harp, J. M., Jewett, F. C. III, Wawrzak, Z., Brown, B. A. III, Rich, A. & Egli, M.. Crystal structure of a luteoviral RNA pseudoknot and model for a minimal ribosomal frameshifting motif. Biochemistry. 2005; 44: 11315-22.

Hanson BL, Langan P, Katz AK, Li X, Harp JM, Glusker JP, Schoenborn BP, Bunick GJ. A preliminary time-of-flight neutron diffraction study of Streptomyces rubiginosus D-xylose isomerase. Acta Crystallogr. D Biol. Crystallogr [print-electronic]. 2004 Feb; 60(Pt 2): 241-9. PMID: 14747699, PII: S0907444903025873, DOI: 10.1107/S0907444903025873, ISSN: 0907-4449.

Hanson BL, Alexander C, Harp JM, Bunick GJ. Preparation and crystallization of nucleosome core particle. Meth. Enzymol. 2004; 375: 44-62. PMID: 14870658, ISSN: 0076-6879.

Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F. Structure of the heterodimeric ecdysone receptor DNA-binding complex. EMBO J. 2003 Nov 11/3/2003; 22(21): 5827-40. PMID: 14592980, PMCID: PMC275426, DOI: 10.1093/emboj/cdg569, ISSN: 0261-4189.

Mi LZ, Devarakonda S, Harp JM, Han Q, Pellicciari R, Willson TM, Khorasanizadeh S, Rastinejad F. Structural basis for bile acid binding and activation of the nuclear receptor FXR. Mol. Cell. 2003 Apr; 11(4): 1093-100. PMID: 12718893, PII: S1097276503001126, ISSN: 1097-2765.

Hanson BL, Harp JM, Bunick GJ. The well-tempered protein crystal: annealing macromolecular crystals. Meth. Enzymol. 2003; 368: 217-35. PMID: 14674276, ISSN: 0076-6879.

Hanson BL, Harp JM, Kirschbaum K, Schall CA, DeWitt K, Howard A, Pinkerton AA, Bunick GJ. Experiments testing the abatement of radiation damage in D-xylose isomerase crystals with cryogenic helium. J Synchrotron Radiat [print-electronic]. 2002 Nov 11/1/2002; 9(Pt 6): 375-81. PMID: 12409625, PII: S0909049502014577, ISSN: 0909-0495.

Jacobs SA, Harp JM, Devarakonda S, Kim Y, Rastinejad F, Khorasanizadeh S. The active site of the SET domain is constructed on a knot. Nat. Struct. Biol. 2002 Nov; 9(11): 833-8. PMID: 12389038, PII: nsb861, DOI: 10.1038/nsb861, ISSN: 1072-8368.

Hanson BL, Bunick GJ, Harp JM, Edmundson AB. Mcg in 2030: new techniques for atomic position determination of immune complexes. J. Mol. Recognit. 2002 Sep; 15(5): 297-305. PMID: 12447907, DOI: 10.1002/jmr.591, ISSN: 0952-3499.

Schurter BT, Koh SS, Chen D, Bunick GJ, Harp JM, Hanson BL, Henschen-Edman A, Mackay DR, Stallcup MR, Aswad DW. Methylation of histone H3 by coactivator-associated arginine methyltransferase 1. Biochemistry. 2001 May 5/15/2001; 40(19): 5747-56. PMID: 11341840, PII: bi002631b, ISSN: 0006-2960.

Harp JM, Hanson BL, Timm DE, Bunick GJ. Asymmetries in the nucleosome core particle at 2.5 A resolution. Acta Crystallogr. D Biol. Crystallogr. 2000 Dec; 56(Pt 12): 1513-34. PMID: 11092917, PII: S0907444900011847, ISSN: 0907-4449.

Timm DE, Mueller HA, Bhanumoorthy P, Harp JM, Bunick GJ. Crystal structure and mechanism of a carbon-carbon bond hydrolase. Structure. 1999 Sep 9/15/1999; 7(9): 1023-33. PMID: 10508789, PII: S0969-2126(99)80170-1, ISSN: 0969-2126.

Harp JM, Hanson BL, Timm DE, Bunick GJ. Macromolecular crystal annealing: evaluation of techniques and variables. Acta Crystallogr. D Biol. Crystallogr. 1999 Jul; 55(Pt 7): 1329-34. PMID: 10393299, PII: GR0906, ISSN: 0907-4449.

Harp JM, Timm DE, Bunick GJ. Macromolecular crystal annealing: overcoming increased mosaicity associated with cryocrystallography. Acta Crystallogr. D Biol. Crystallogr. 1998 Jul 7/1/1998; 54(Pt 4): 622-8. PMID: 9761858, ISSN: 0907-4449.

Harp JM, Uberbacher EC, Roberson AE, Palmer EL, Gewiess A, Bunick GJ. X-ray diffraction analysis of crystals containing twofold symmetric nucleosome core particles. Acta Crystallogr. D Biol. Crystallogr. 1996 Mar 3/1/1996; 52(Pt 2): 283-8. PMID: 15299701, PII: S0907444995009139, DOI: 10.1107/S0907444995009139, ISSN: 0907-4449.

Palmer EL, Gewiess A, Harp JM, York MH, Bunick GJ. Large-scale production of palindrome DNA fragments. Anal. Biochem. 1995 Oct 10/10/1995; 231(1): 109-14. PMID: 8678288, PII: S0003-2697(85)71509-6, DOI: 10.1006/abio.1995.1509, ISSN: 0003-2697.

Harp JM, Palmer EL, York MH, Gewiess A, Davis M, Bunick GJ. Preparative separation of nucleosome core particles containing defined-sequence DNA in multiple translational phases. Electrophoresis. 1995 Oct; 16(10): 1861-4. PMID: 8586054, ISSN: 0173-0835.

Uberbacher EC, Harp JM, Bunick GJ. DNA sequence patterns in precisely positioned nucleosomes. J. Biomol. Struct. Dyn. 1988 Aug; 6(1): 105-20. PMID: 3271513, DOI: 10.1080/07391102.1988.10506485, ISSN: 0739-1102.

Uberbacher EC, Harp JM, Wilkinson-Singley E, Bunick GJ. Shape analysis of the histone octamer in solution. Science. 1986 Jun 6/6/1986; 232(4755): 1247-9. PMID: 3704649, ISSN: 0036-8075.

Allen RL, Olins AL, Harp JM, Olins DE. Isolation and characterization of chromatin replication bands and macronuclei from Euplotes eurystomus. Eur. J. Cell Biol. 1985 Nov; 39(1): 217-23. PMID: 2417842, ISSN: 0171-9335.

[place, publisher, date unknown].