Ana Marin Dias Carneiro, Ph.D.

Assistant Professor

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Faculty Appointments
Assistant Professor of Pharmacology
M.A., Biochemistry and Immunology, Universidade Federal de Minas Gerais , Belo Horizonte, BrazilPh.D., Biochemistry and Immunology, Universidade Federal de Minas Gerais , Belo Horizonte, BrazilB.S., Universidade Federal de Minas Gerais , Belo Horizonte, Brazil
Office Address
461 PRB
23rd South At Pierce
Nashville, TN 37232-6600
Research Description
My laboratory studies the molecular mechanisms by which integrins protein complexes influence cell function and behavior. Integrins coordinate cell surface receptors, neurotransmitter transporters and intracellular signaling proteins in a tissue-dependent manner. Therefore, first we need to figure out which signaling networks and proteins are modulated by integrins. We focus on the discovery and characterization of protein-protein interactions through biochemical and proteomic approaches. These findings are then taken to the whole animal, where we generate genetically modified mice or disrupt the protein complexes by pharmacological manipulation and measure the outcome in behavioral studies.
Research Keywords
biochemistry, integrins, neurotransmitter transporter, dopamine, serotonin, depression, stress
Mazalouskas M, Jessen T, Varney S, Sutcliffe JS, Veenstra-VanderWeele J, Cook EH, Carneiro AM. Integrin ß3 Haploinsufficiency Modulates Serotonin Transport and Antidepressant-Sensitive Behavior in Mice. Neuropsychopharmacology [print-electronic]. 2015 Jul; 40(8): 2015-24. PMID: 25684064, PII: npp201551, DOI: 10.1038/npp.2015.51, ISSN: 1740-634X.

Varney S, Polston KF, Jessen T, Carneiro AM. Mice lacking integrin ß3 expression exhibit altered response to chronic stress. Neurobiol Stress [print-electronic]. 2015; 2: 51-8. PMID: 26634222, PMCID: PMC4664197, DOI: 10.1016/j.ynstr.2015.05.002, ISSN: 2352-2895.

Whyte A, Jessen T, Varney S, Carneiro AM. Serotonin transporter and integrin beta 3 genes interact to modulate serotonin uptake in mouse brain. Neurochem. Int [print-electronic]. 2014 Jul; 73: 122-6. PMID: 24083985, PMCID: PMC3969782, PII: S0197-0186(13)00241-6, DOI: 10.1016/j.neuint.2013.09.014, ISSN: 1872-9754.

Oliver KH, Jessen T, Crawford EL, Chung CY, Sutcliffe JS, Carneiro AM. Pro32Pro33 mutations in the integrin ß3 PSI domain result in aIIbß3 priming and enhanced adhesion: reversal of the hypercoagulability phenotype by the Src inhibitor SKI-606. Mol. Pharmacol [print-electronic]. 2014 Jun; 85(6): 921-31. PMID: 24695082, PMCID: PMC4014669, PII: mol.114.091736, DOI: 10.1124/mol.114.091736, ISSN: 1521-0111.

Ye R, Carneiro AM, Han Q, Airey D, Sanders-Bush E, Zhang B, Lu L, Williams R, Blakely RD. Quantitative trait loci mapping and gene network analysis implicate protocadherin-15 as a determinant of brain serotonin transporter expression. Genes Brain Behav [print-electronic]. 2014 Mar; 13(3): 261-75. PMID: 24405699, PMCID: PMC4436591, DOI: 10.1111/gbb.12119, ISSN: 1601-183X.

Ye R, Carneiro AM, Airey D, Sanders-Bush E, Williams RW, Lu L, Wang J, Zhang B, Blakely RD. Evaluation of heritable determinants of blood and brain serotonin homeostasis using recombinant inbred mice. Genes Brain Behav [print-electronic]. 2014 Mar; 13(3): 247-60. PMID: 24102824, PMCID: PMC3979832, DOI: 10.1111/gbb.12092, ISSN: 1601-183X.

Chang JC, Tomlinson ID, Warnement MR, Ustione A, Carneiro AM, Piston DW, Blakely RD, Rosenthal SJ. Single molecule analysis of serotonin transporter regulation using antagonist-conjugated quantum dots reveals restricted, p38 MAPK-dependent mobilization underlying uptake activation. J. Neurosci. 2012 Jun 6/27/2012; 32(26): 8919-29. PMID: 22745492, PMCID: PMC3426861, PII: 32/26/8919, DOI: 10.1523/JNEUROSCI.0048-12.2012, ISSN: 1529-2401.

Veenstra-VanderWeele J, Muller CL, Iwamoto H, Sauer JE, Owens WA, Shah CR, Cohen J, Mannangatti P, Jessen T, Thompson BJ, Ye R, Kerr TM, Carneiro AM, Crawley JN, Sanders-Bush E, McMahon DG, Ramamoorthy S, Daws LC, Sutcliffe JS, Blakely RD. Autism gene variant causes hyperserotonemia, serotonin receptor hypersensitivity, social impairment and repetitive behavior. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Apr 4/3/2012; 109(14): 5469-74. PMID: 22431635, PMCID: PMC3325657, PII: 1112345109, DOI: 10.1073/pnas.1112345109, ISSN: 1091-6490.

Thompson BJ, Jessen T, Henry LK, Field JR, Gamble KL, Gresch PJ, Carneiro AM, Horton RE, Chisnell PJ, Belova Y, McMahon DG, Daws LC, Blakely RD. Transgenic elimination of high-affinity antidepressant and cocaine sensitivity in the presynaptic serotonin transporter. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2011 Mar 3/1/2011; 108(9): 3785-90. PMID: 21282638, PMCID: PMC3048100, PII: 1011920108, DOI: 10.1073/pnas.1011920108, ISSN: 1091-6490.

Carter MD, Shah CR, Muller CL, Crawley JN, Carneiro AM, Veenstra-VanderWeele J. Absence of preference for social novelty and increased grooming in integrin ß3 knockout mice: initial studies and future directions. Autism Res [print-electronic]. 2011 Feb; 4(1): 57-67. PMID: 21254450, PMCID: PMC3073711, DOI: 10.1002/aur.180, ISSN: 1939-3806.

Carneiro AM. The emerging role of integrins in neuropsychiatric disorders. Neuropsychopharmacology. 2010 Jan; 35(1): 338-9. PMID: 20010705, PMCID: PMC3055418, PII: npp2009134, DOI: 10.1038/npp.2009.134, ISSN: 1740-634X.

Carneiro AM, Airey DC, Thompson B, Zhu CB, Lu L, Chesler EJ, Erikson KM, Blakely RD. Functional coding variation in recombinant inbred mouse lines reveals multiple serotonin transporter-associated phenotypes. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2009 Feb 2/10/2009; 106(6): 2047-52. PMID: 19179283, PMCID: PMC2632716, PII: 0809449106, DOI: 10.1073/pnas.0809449106, ISSN: 1091-6490.

Steiner JA, Carneiro AM, Wright J, Matthies HJ, Prasad HC, Nicki CK, Dostmann WR, Buchanan CC, Corbin JD, Francis SH, Blakely RD. CGMP-dependent protein kinase Ialpha associates with the antidepressant-sensitive serotonin transporter and dictates rapid modulation of serotonin uptake. Mol Brain. 2009; 2: 26. PMID: 19656393, PMCID: PMC2731736, PII: 1756-6606-2-26, DOI: 10.1186/1756-6606-2-26, ISSN: 1756-6606.

Steiner JA, Carneiro AM, Blakely RD. Going with the flow: trafficking-dependent and -independent regulation of serotonin transport. Traffic [print-electronic]. 2008 Sep; 9(9): 1393-402. PMID: 18445122, PMCID: PMC2773847, PII: TRA757, DOI: 10.1111/j.1600-0854.2008.00757.x, ISSN: 1600-0854.

Carneiro AM, Cook EH, Murphy DL, Blakely RD. Interactions between integrin alphaIIbbeta3 and the serotonin transporter regulate serotonin transport and platelet aggregation in mice and humans. J. Clin. Invest. 2008 Apr; 118(4): 1544-52. PMID: 18317590, PMCID: PMC2260909, DOI: 10.1172/JCI33374, ISSN: 0021-9738.

Carneiro AM, Blakely RD. Serotonin-, protein kinase C-, and Hic-5-associated redistribution of the platelet serotonin transporter. J. Biol. Chem [print-electronic]. 2006 Aug 8/25/2006; 281(34): 24769-80. PMID: 16803896, PMCID: PMC3875312, PII: M603877200, DOI: 10.1074/jbc.M603877200, ISSN: 0021-9258.

Zhu CB, Carneiro AM, Dostmann WR, Hewlett WA, Blakely RD. P38 MAPK activation elevates serotonin transport activity via a trafficking-independent, protein phosphatase 2A-dependent process. J. Biol. Chem [print-electronic]. 2005 Apr 4/22/2005; 280(16): 15649-58. PMID: 15728187, PII: M410858200, DOI: 10.1074/jbc.M410858200, ISSN: 0021-9258.