Kathleen L. Gould, Ph.D.



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Faculty Appointments
Professor of Cell and Developmental Biology Louise B. McGavock Chair
Ph.D., Biology, University of California, San Diego, CaliforniaA.B., Biochemistry, University of California, Berkeley, California
Office Address
B-2309 MCN
1161 21st Ave S
Nashville 37232-8240
Research Description
The goals of my laboratory are two-fold: 1) to understand the molecular mechanisms driving cytokinesis and 2) to learn how cytokinesis is coordinated with other events of mitosis including chromosome segregation. We are asking a) what is the nano-architecture of a contractile ring, b) how is the cytokinetic machinery precisely localized between segregating chromosomes, c) what molecular signal triggers constriction of the acto-myosin machinery, and d) what ensures the correct timing of cytokinesis with respect to chromosome segregation?

To address these questions expeditiously, we use yeast as a model organism. Yeast offers many experimental advantages for the study of cell cycle regulation including facile genetics and a fully sequenced and annotated genome. Using super-resolution imaging, proteomics, live cell imaging, genetics, biochemistry, and structural biology we are unraveling the sequence of events that directs formation and then constriction of the cytokinetic ring. We also study protein phosphorylation-based signaling pathways and ubiquitin ligases that control the spatial and temporal precision of cytokinesis.
Research Keywords
cell cycle, cytokinesis, phosphoproteomics/mass spectrometry, super-resolution imaging, signal transduction, checkpoint signaling, ubiquitination, protein kinases
Snider CE, Willet AH, Chen JS, Arpag G, Zanic M, Gould KL. Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis. J. Cell Biol [print-electronic]. 2017 Aug 8/7/2017; PMID: 28784611, PII: jcb.201705070, DOI: 10.1083/jcb.201705070, ISSN: 1540-8140.

Petrie KA, Carnahan RH, Brown AM, Gould KL. Providing Experiential Business and Management Training for Biomedical Research Trainees. CBE Life Sci Educ. 2017; 16(3): PMID: 28798213, PII: 16/3/ar51, DOI: 10.1187/cbe.17-05-0074, ISSN: 1931-7913.

Pyburn TM, Foegeding NJ, González-Rivera C, McDonald NA, Gould KL, Cover TL, Ohi MD. Structural organization of membrane-inserted hexamers formed by Helicobacter pylori VacA toxin. Mol. Microbiol [print-electronic]. 2016 Jun 6/16/2016; PMID: 27309820, DOI: 10.1111/mmi.13443, ISSN: 1365-2958.

McDonald NA, Gould KL. Linking up at the BAR: Oligomerization and F-BAR protein function. Cell Cycle [print-electronic]. 2016 May 5/31/2016; 1-9. PMID: 27245932, DOI: 10.1080/15384101.2016.1190893, ISSN: 1551-4005.

Chen JS, Beckley JR, Ren L, Feoktistova A, Jensen MA, Rhind N, Gould KL. Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains. Yeast [print-electronic]. 2016 May 5/11/2016; PMID: 27168121, DOI: 10.1002/yea.3172, ISSN: 1097-0061.

McDonald NA, Takizawa Y, Feoktistova A, Xu P, Ohi MD, Vander Kooi CW, Gould KL. The Tubulation Activity of a Fission Yeast F-BAR Protein Is Dispensable for Its Function in Cytokinesis. Cell Rep [print-electronic]. 2016 Jan 1/26/2016; 14(3): 534-46. PMID: 26776521, PMCID: PMC4731314, PII: S2211-1247(15)01503-X, DOI: 10.1016/j.celrep.2015.12.062, ISSN: 2211-1247.

Makarova M, Gu Y, Chen JS, Beckley JR, Gould KL, Oliferenko S. Temporal Regulation of Lipin Activity Diverged to Account for Differences in Mitotic Programs. Curr. Biol [print-electronic]. 2016 Jan 1/25/2016; 26(2): 237-43. PMID: 26774782, PII: S0960-9822(15)01509-2, DOI: 10.1016/j.cub.2015.11.061, ISSN: 1879-0445.

McDonald NA, Gould KL. Characterization of Cytokinetic F-BARs and Other Membrane-Binding Proteins. Methods Mol. Biol. 2016; 1369: 181-9. PMID: 26519313, DOI: 10.1007/978-1-4939-3145-3_13, ISSN: 1940-6029.

McDonald NA, Vander Kooi CW, Ohi MD, Gould KL. Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Motility and Cytokinesis. Dev. Cell. 2015 Dec 12/21/2015; 35(6): 725-36. PMID: 26702831, PMCID: PMC4691284, PII: S1534-5807(15)00758-3, DOI: 10.1016/j.devcel.2015.11.023, ISSN: 1878-1551.

Meyers FJ, Mathur A, Fuhrmann CN, O'Brien TC, Wefes I, Labosky PA, Duncan DS, August A, Feig A, Gould KL, Friedlander MJ, Schaffer CB, Van Wart A, Chalkley R. The origin and implementation of the Broadening Experiences in Scientific Training programs: an NIH common fund initiative. FASEB J [print-electronic]. 2015 Oct 10/2/2015; PMID: 26432783, PII: fj.15-276139, DOI: 10.1096/fj.15-276139, ISSN: 1530-6860.

Beckley JR, Chen JS, Yang Y, Peng J, Gould KL. A degenerate cohort of yeast membrane trafficking DUBs mediates cell polarity and survival. Mol. Cell Proteomics [print-electronic]. 2015 Sep 9/27/2015; PMID: 26412298, PII: M115.050039, DOI: 10.1074/mcp.M115.050039, ISSN: 1535-9484.

Willet AH, McDonald NA, Gould KL. Regulation of contractile ring formation and septation in Schizosaccharomyces pombe. Curr. Opin. Microbiol [print-electronic]. 2015 Sep 9/1/2015; 28: 46-52. PMID: 26340438, PII: S1369-5274(15)00106-X, DOI: 10.1016/j.mib.2015.08.001, ISSN: 1879-0364.

Chen JS, Beckley JR, McDonald NA, Ren L, Mangione M, Jang SJ, Elmore ZC, Rachfall N, Feoktistova A, Jones CM, Willet AH, Guillen R, Bitton DA, Bähler J, Jensen MA, Rhind N, Gould KL. Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains. G3 (Bethesda). 2015 Mar; 5(3): 361-70. PMID: 25552606, PMCID: PMC4349090, PII: g3.114.015701, DOI: 10.1534/g3.114.015701, ISSN: 2160-1836.

Willet AH, McDonald NA, Bohnert KA, Baird MA, Allen JR, Davidson MW, Gould KL. The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc12. J. Cell Biol. 2015 Feb 2/16/2015; 208(4): 391-9. PMID: 25688133, PMCID: PMC4332253, PII: jcb.201411097, DOI: 10.1083/jcb.201411097, ISSN: 1540-8140.

Ren L, Willet AH, Roberts-Galbraith RH, McDonald NA, Feoktistova A, Chen JS, Huang H, Guillen R, Boone C, Sidhu SS, Beckley JR, Gould KL. The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast. Mol. Biol. Cell [print-electronic]. 2015 Jan 1/15/2015; 26(2): 256-69. PMID: 25428987, PMCID: PMC4294673, PII: mbc.E14-10-1451, DOI: 10.1091/mbc.E14-10-1451, ISSN: 1939-4586.

Collier SE, Voehler M, Peng D, Ohi R, Gould KL, Reiter NJ, Ohi MD. Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Biochemistry [print-electronic]. 2014 Oct 10/21/2014; 53(41): 6439-51. PMID: 25263959, PMCID: PMC4204884, DOI: 10.1021/bi5008639, ISSN: 1520-4995.

Elmore ZC, Beckley JR, Chen JS, Gould KL. Histone H2B ubiquitination promotes the function of the anaphase-promoting complex/cyclosome in Schizosaccharomyces pombe. G3 (Bethesda). 2014 Aug; 4(8): 1529-38. PMID: 24948786, PMCID: PMC4132182, PII: g3.114.012625, DOI: 10.1534/g3.114.012625, ISSN: 2160-1836.

Rachfall N, Johnson AE, Mehta S, Chen JS, Gould KL. Cdk1 promotes cytokinesis in fission yeast through activation of the septation initiation network. Mol. Biol. Cell [print-electronic]. 2014 Aug 8/1/2014; 25(15): 2250-9. PMID: 24920823, PMCID: PMC4116299, PII: mbc.E14-04-0936, DOI: 10.1091/mbc.E14-04-0936, ISSN: 1939-4586.

Bohnert KA, Willet AH, Kovar DR, Gould KL. Formin-based control of the actin cytoskeleton during cytokinesis. Biochem. Soc. Trans. 2013 Dec; 41(6): 1750-4. PMID: 24256286, PII: BST20130208, DOI: 10.1042/BST20130208, ISSN: 1470-8752.

Johnson AE, Chen JS, Gould KL. CK1 is required for a mitotic checkpoint that delays cytokinesis. Curr. Biol [print-electronic]. 2013 Oct 10/7/2013; 23(19): 1920-6. PMID: 24055157, PMCID: PMC4078987, PII: S0960-9822(13)00938-X, DOI: 10.1016/j.cub.2013.07.077, ISSN: 1879-0445.

Bohnert KA, Grzegorzewska AP, Willet AH, Vander Kooi CW, Kovar DR, Gould KL. SIN-dependent phosphoinhibition of formin multimerization controls fission yeast cytokinesis. Genes Dev. 2013 Oct 10/1/2013; 27(19): 2164-77. PMID: 24115772, PMCID: PMC3850099, PII: 27/19/2164, DOI: 10.1101/gad.224154.113, ISSN: 1549-5477.

Chen JS, Broadus MR, McLean JR, Feoktistova A, Ren L, Gould KL. Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase. Mol. Cell Proteomics [print-electronic]. 2013 May; 12(5): 1074-86. PMID: 23297348, PMCID: PMC3650322, PII: M112.025924, DOI: 10.1074/mcp.M112.025924, ISSN: 1535-9484.

Dephoure N, Gould KL, Gygi SP, Kellogg DR. Mapping and analysis of phosphorylation sites: a quick guide for cell biologists. Mol. Biol. Cell. 2013 Mar; 24(5): 535-42. PMID: 23447708, PMCID: PMC3583658, PII: 24/5/535, DOI: 10.1091/mbc.E12-09-0677, ISSN: 1939-4586.

Gupta S, Mana-Capelli S, McLean JR, Chen CT, Ray S, Gould KL, McCollum D. Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways. Curr. Biol [print-electronic]. 2013 Feb 2/18/2013; 23(4): 333-8. PMID: 23394829, PMCID: PMC3580008, PII: S0960-9822(13)00017-1, DOI: 10.1016/j.cub.2013.01.014, ISSN: 1879-0445.

Bajpai A, Feoktistova A, Chen JS, McCollum D, Sato M, Carazo-Salas RE, Gould KL, Csikász-Nagy A. Dynamics of SIN asymmetry establishment. PLoS Comput. Biol [print-electronic]. 2013; 9(7): e1003147. PMID: 23874188, PMCID: PMC3708865, PII: PCOMPBIOL-D-13-00179, DOI: 10.1371/journal.pcbi.1003147, ISSN: 1553-7358.

Clément A, Solnica-Krezel L, Gould KL. Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis. Dev. Dyn [print-electronic]. 2012 Dec; 241(12): 1911-21. PMID: 23027426, PMCID: PMC3508521, DOI: 10.1002/dvdy.23876, ISSN: 1097-0177.

Mana-Capelli S, McLean JR, Chen CT, Gould KL, McCollum D. The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning. Mol. Biol. Cell [print-electronic]. 2012 Dec; 23(23): 4592-600. PMID: 23087209, PMCID: PMC3510020, PII: mbc.E12-07-0532, DOI: 10.1091/mbc.E12-07-0532, ISSN: 1939-4586.

Johnson AE, McCollum D, Gould KL. Polar opposites: Fine-tuning cytokinesis through SIN asymmetry. Cytoskeleton (Hoboken) [print-electronic]. 2012 Oct; 69(10): 686-99. PMID: 22786806, PMCID: PMC3478943, DOI: 10.1002/cm.21044, ISSN: 1949-3592.

Broadus MR, Gould KL. Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon genotoxic stress. Mol. Biol. Cell [print-electronic]. 2012 Oct; 23(20): 4118-28. PMID: 22918952, PMCID: PMC3469525, PII: mbc.E12-06-0475, DOI: 10.1091/mbc.E12-06-0475, ISSN: 1939-4586.

Wang Y, Li WZ, Johnson AE, Luo ZQ, Sun XL, Feoktistova A, McDonald WH, McLeod I, Yates JR, Gould KL, McCollum D, Jin QW. Dnt1 acts as a mitotic inhibitor of the spindle checkpoint protein dma1 in fission yeast. Mol. Biol. Cell [print-electronic]. 2012 Sep; 23(17): 3348-56. PMID: 22809626, PMCID: PMC3431938, PII: mbc.E11-12-1020, DOI: 10.1091/mbc.E11-12-1020, ISSN: 1939-4586.

Johnson AE, Collier SE, Ohi MD, Gould KL. Fission yeast Dma1 requires RING domain dimerization for its ubiquitin ligase activity and mitotic checkpoint function. J. Biol. Chem [print-electronic]. 2012 Jul 7/27/2012; 287(31): 25741-8. PMID: 22669973, PMCID: PMC3406662, PII: M112.349712, DOI: 10.1074/jbc.M112.349712, ISSN: 1083-351X.

Lu LX, Domingo-Sananes MR, Huzarska M, Novak B, Gould KL. Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Jun 6/19/2012; 109(25): 9899-904. PMID: 22665807, PMCID: PMC3382524, PII: 1201366109, DOI: 10.1073/pnas.1201366109, ISSN: 1091-6490.

Feoktistova A, Morrell-Falvey J, Chen JS, Singh NS, Balasubramanian MK, Gould KL. The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11. Mol. Biol. Cell [print-electronic]. 2012 May; 23(9): 1636-45. PMID: 22419817, PMCID: PMC3338431, PII: mbc.E11-09-0792, DOI: 10.1091/mbc.E11-09-0792, ISSN: 1939-4586.

Bohnert KA, Gould KL. Cytokinesis-based constraints on polarized cell growth in fission yeast. PLoS Genet [print-electronic]. 2012; 8(10): e1003004. PMID: 23093943, PMCID: PMC3475658, PII: PGENETICS-D-12-00944, DOI: 10.1371/journal.pgen.1003004, ISSN: 1553-7404.

Wang, Y, Li, WZ, Johnson, AE, Luo, ZQ, Sun, L, Feoktistova, A, McDonald, WH, McLeod, I, Yates, III, JR, Gould, KL, McCollum, D, and Jin, QW. Dnt1 Acts as a Mitotic Inhibitor of the Spindle Checkpoint Protein Dma1 in Fission Yeast. Mol Biol Cell. 2012; 23((17)): 3338-56.

Ma H, McLean JR, Chao LF, Mana-Capelli S, Paramasivam M, Hagstrom KA, Gould KL, McCollum D.. A highly efficient multifunctional tandem affinity purification approachapplicable to diverse organisms. Mol Cell Proteomics. 2012; 11((8)): 501-11.

Pancaldi V, Saraç OS, Rallis C, McLean JR, Převorovský M, Gould K, Beyer A, Bähler J.. Predicting the fission yeast protein interaction network. G3: Genes, Genomes and Genetics. 2012; 2((4)): 453-67.

Chen JS, Lu LX, Ohi MD, Creamer KM, English C, Partridge JF, Ohi R, Gould KL. Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J. Cell Biol [print-electronic]. 2011 Nov 11/14/2011; 195(4): 583-93. PMID: 22065639, PMCID: PMC3257533, PII: jcb.201105074, DOI: 10.1083/jcb.201105074, ISSN: 1540-8140.

Peckys DB, Mazur P, Gould KL, de Jonge N. Fully hydrated yeast cells imaged with electron microscopy. Biophys. J. 2011 May 5/18/2011; 100(10): 2522-9. PMID: 21575587, PMCID: PMC3093553, PII: S0006-3495(11)00403-6, DOI: 10.1016/j.bpj.2011.03.045, ISSN: 1542-0086.

Bohnert KA, Gould KL. On the cutting edge: post-translational modifications in cytokinesis. Trends Cell Biol [print-electronic]. 2011 May; 21(5): 283-92. PMID: 21349716, PII: S0962-8924(11)00016-X, DOI: 10.1016/j.tcb.2011.01.006, ISSN: 1879-3088.

McLean JR, Chaix D, Ohi MD, Gould KL. State of the APC/C: organization, function, and structure. Crit. Rev. Biochem. Mol. Biol [print-electronic]. 2011 Apr; 46(2): 118-36. PMID: 21261459, DOI: 10.3109/10409238.2010.541420, ISSN: 1549-7798.

McLean JR, Kouranti I, Gould KL. Survey of the phosphorylation status of the Schizosaccharomyces pombe deubiquitinating enzyme (DUB) family. J. Proteome Res [print-electronic]. 2011 Mar 3/4/2011; 10(3): 1208-15. PMID: 21182284, PMCID: PMC3049645, DOI: 10.1021/pr100985s, ISSN: 1535-3907.

Goettel JA, Liang D, Hilliard VC, Edelblum KL, Broadus MR, Gould KL, Hanks SK, Polk DB. KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation of MEK1. Exp. Cell Res [print-electronic]. 2011 Feb 2/15/2011; 317(4): 452-63. PMID: 21144847, PMCID: PMC3038243, PII: S0014-4827(10)00547-1, DOI: 10.1016/j.yexcr.2010.11.018, ISSN: 1090-2422.

Johnson AE, Gould KL. Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase. EMBO J [print-electronic]. 2011 Jan 1/19/2011; 30(2): 341-54. PMID: 21131906, PMCID: PMC3025462, PII: emboj2010317, DOI: 10.1038/emboj.2010.317, ISSN: 1460-2075.

Clément A, Solnica-Krezel L, Gould KL. The Cdc14B phosphatase contributes to ciliogenesis in zebrafish. Development. 2011 Jan; 138(2): 291-302. PMID: 21177342, PMCID: PMC3005604, PII: 138/2/291, DOI: 10.1242/dev.055038, ISSN: 1477-9129.

Ren L, McLean JR, Hazbun TR, Fields S, Vander Kooi C, Ohi MD, Gould KL. Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. PLoS ONE. 2011; 6(2): e16719. PMID: 21386897, PMCID: PMC3046128, DOI: 10.1371/journal.pone.0016719, ISSN: 1932-6203.

Almonacid M, Celton-Morizur S, Jakubowski JL, Dingli F, Loew D, Mayeux A, Chen JS, Gould KL, Clifford DM, Paoletti A.. Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin. Cur Biol. 2011; 21((6)): 473-9.

Prevorovsky, M, Atkinson, SR, Ptackova, M, McLean, JR, Gould, KL, Folk, P, Puta, F, and Bahler, J. N-termini of fungal CSL transcription factors are dirordered, enriched in regulatory motifs and inhibit DNA binding in fission yeast. PLoS ONE. 2011; 6: e23650.

Singh NS, Shao N, McLean JR, Sevugan M, Ren L, Chew TG, Bimbo A, Sharma R, Tang X, Gould KL, Balasubramanian MK.. SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast. Curr Biol. 2011; 21((23)): 1968-78.

Roberts-Galbraith RH, Gould KL. Setting the F-BAR: functions and regulation of the F-BAR protein family. Cell Cycle [print-electronic]. 2010 Oct 10/15/2010; 9(20): 4091-7. PMID: 20948299, PII: 13587, ISSN: 1551-4005.

Roberts-Galbraith RH, Ohi MD, Ballif BA, Chen JS, McLeod I, McDonald WH, Gygi SP, Yates JR, Gould KL. Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site. Mol. Cell. 2010 Jul 7/9/2010; 39(1): 86-99. PMID: 20603077, PMCID: PMC2916701, PII: S1097-2765(10)00453-3, DOI: 10.1016/j.molcel.2010.06.012, ISSN: 1097-4164.

Vander Kooi CW, Ren L, Xu P, Ohi MD, Gould KL, Chazin WJ. The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Structure. 2010 May 5/12/2010; 18(5): 584-93. PMID: 20462492, PMCID: PMC2871033, PII: S0969-2126(10)00109-7, DOI: 10.1016/j.str.2010.02.015, ISSN: 1878-4186.

Kouranti I, McLean JR, Feoktistova A, Liang P, Johnson AE, Roberts-Galbraith RH, Gould KL. A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity. PLoS Biol. 2010; 8(9): PMID: 20838651, PMCID: PMC2935449, DOI: 10.1371/journal.pbio.1000471, ISSN: 1545-7885.

Papadopoulou K, Chen JS, Mead E, Feoktistova A, Petit C, Agarwal M, Jamal M, Malik A, Spanos A, Sedgwick SG, Karagiannis J, Balasubramanian MK, Gould KL, McInerny CJ.. Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p. J Cell Sci. 2010; 123((Pt 24)): 4374-81.

Bohnert KA, Chen JS, Clifford DM, Vander Kooi CW, Gould KL. A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission yeast borealin-like protein. Mol. Biol. Cell [print-electronic]. 2009 Aug; 20(16): 3646-59. PMID: 19570910, PMCID: PMC2777925, PII: E09-04-0289, DOI: 10.1091/mbc.E09-04-0289, ISSN: 1939-4586.

Roberts-Galbraith RH, Chen JS, Wang J, Gould KL.. The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring. J Cell Biol. 2009; 184(1): 113-27.

Roberts-Galbraith RH, Gould KL. Stepping into the ring: the SIN takes on contractile ring assembly. Genes Dev. 2008 Nov 11/15/2008; 22(22): 3082-8. PMID: 19056889, PII: 22/22/3082, DOI: 10.1101/gad.1748908, ISSN: 0890-9369.

Clifford DM, Chen CT, Roberts RH, Feoktistova A, Wolfe BA, Chen JS, McCollum D, Gould KL. The role of Cdc14 phosphatases in the control of cell division. Biochem. Soc. Trans. 2008 Jun; 36(Pt 3): 436-8. PMID: 18481975, PII: BST0360436, DOI: 10.1042/BST0360436, ISSN: 0300-5127.

Clifford DM, Wolfe BA, Roberts-Galbraith RH, McDonald WH, Yates JR, Gould KL. The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1. J. Cell Biol [print-electronic]. 2008 Apr 4/7/2008; 181(1): 79-88. PMID: 18378776, PMCID: PMC2287289, PII: jcb.200709060, DOI: 10.1083/jcb.200709060, ISSN: 1540-8140.

Sczaniecka M, Feoktistova A, May KM, Chen JS, Blyth J, Gould KL, Hardwick KG.. The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem. 2008; 283(34): 23039-47.

Chen CT, Feoktistova A, Chen JS, Shim YS, Clifford DM, Gould KL, McCollum D.. The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1. Curr Biol. 2008; 18((20)): 1594-9.

Ohi MD, Ren L, Wall JS, Gould KL, Walz T. Structural characterization of the fission yeast U5.U2/U6 spliceosome complex. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2007 Feb 2/27/2007; 104(9): 3195-200. PMID: 17360628, PMCID: PMC1805518, PII: 0611591104, DOI: 10.1073/pnas.0611591104, ISSN: 0027-8424.

Ohi, M.D., Feoktistova, A. Ren, L., Yip, C., Cheng, Y., Chen, J.-S., Yoon, H.-J., Wall, J.S., Huang, Z., Penczek, P.A., Gould, K.L.*, and Walz, T.*. Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Mol Cell. 2007; 28: 871-85.

Yoon HJ, Feoktistova A, Chen JS, Jennings JL, Link AJ, Gould KL. Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function. J. Biol. Chem [print-electronic]. 2006 Oct 10/27/2006; 281(43): 32284-93. PMID: 16950791, PII: M603867200, DOI: 10.1074/jbc.M603867200, ISSN: 0021-9258.

Wolfe BA, McDonald WH, Yates JR, Gould KL. Phospho-regulation of the Cdc14/Clp1 phosphatase delays late mitotic events in S. pombe. Dev. Cell. 2006 Sep; 11(3): 423-30. PMID: 16950131, PII: S1534-5807(06)00345-5, DOI: 10.1016/j.devcel.2006.07.016, ISSN: 1534-5807.

Rosenberg JA, Tomlin GC, McDonald WH, Snydsman BE, Muller EG, Yates JR, Gould KL. Ppc89 links multiple proteins, including the septation initiation network, to the core of the fission yeast spindle-pole body. Mol. Biol. Cell [print-electronic]. 2006 Sep; 17(9): 3793-805. PMID: 16775007, PMCID: PMC1593159, PII: E06-01-0039, DOI: 10.1091/mbc.E06-01-0039, ISSN: 1059-1524.

Mehta S, Gould KL. Identification of functional domains within the septation initiation network kinase, Cdc7. J. Biol. Chem [print-electronic]. 2006 Apr 4/14/2006; 281(15): 9935-41. PMID: 16469735, PII: M600160200, DOI: 10.1074/jbc.M600160200, ISSN: 0021-9258.

Vander Kooi CW, Ohi MD, Rosenberg JA, Oldham ML, Newcomer ME, Gould KL, Chazin WJ. The Prp19 U-box crystal structure suggests a common dimeric architecture for a class of oligomeric E3 ubiquitin ligases. Biochemistry. 2006 Jan 1/10/2006; 45(1): 121-30. PMID: 16388587, PMCID: PMC2570371, DOI: 10.1021/bi051787e, ISSN: 0006-2960.

Petit CS, Mehta S, Roberts RH, Gould KL. Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression. J. Cell. Sci [print-electronic]. 2005 Dec 12/15/2005; 118(Pt 24): 5731-42. PMID: 16317047, PII: jcs.02687, DOI: 10.1242/jcs.02687, ISSN: 0021-9533.

Morrell-Falvey JL, Ren L, Feoktistova A, Haese GD, Gould KL. Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p. J. Cell. Sci [print-electronic]. 2005 Dec 12/1/2005; 118(Pt 23): 5563-73. PMID: 16291723, PII: jcs.02664, DOI: 10.1242/jcs.02664, ISSN: 0021-9533.

Martín-Cuadrado AB, Morrell JL, Konomi M, An H, Petit C, Osumi M, Balasubramanian M, Gould KL, Del Rey F, de Aldana CR. Role of septins and the exocyst complex in the function of hydrolytic enzymes responsible for fission yeast cell separation. Mol. Biol. Cell [print-electronic]. 2005 Oct; 16(10): 4867-81. PMID: 16079182, PMCID: PMC1237089, PII: E04-12-1114, DOI: 10.1091/mbc.E04-12-1114, ISSN: 1059-1524.

Venkatram S, Jennings JL, Link A, Gould KL. Mto2p, a novel fission yeast protein required for cytoplasmic microtubule organization and anchoring of the cytokinetic actin ring. Mol. Biol. Cell [print-electronic]. 2005 Jun; 16(6): 3052-63. PMID: 15800064, PMCID: PMC1142447, PII: E04-12-1043, DOI: 10.1091/mbc.E04-12-1043, ISSN: 1059-1524.

Ren L, Feoktistova A, McDonald WH, Haese GD, Morrell JL, Gould KL. Analysis of the role of phosphorylation in fission yeast Cdc13p/cyclinB function. J. Biol. Chem [print-electronic]. 2005 Apr 4/15/2005; 280(15): 14591-6. PMID: 15705571, PII: M500560200, DOI: 10.1074/jbc.M500560200, ISSN: 0021-9258.

Alonso-Nuñez ML, An H, Martín-Cuadrado AB, Mehta S, Petit C, Sipiczki M, del Rey F, Gould KL, de Aldana CR. Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe. Mol. Biol. Cell [print-electronic]. 2005 Apr; 16(4): 2003-17. PMID: 15689498, PMCID: PMC1073678, PII: E04-06-0442, DOI: 10.1091/mbc.E04-06-0442, ISSN: 1059-1524.

Carnahan RH, Feoktistova A, Ren L, Niessen S, Yates JR, Gould KL. Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex. Eukaryotic Cell. 2005 Mar; 4(3): 577-87. PMID: 15755920, PMCID: PMC1087801, PII: 4/3/577, DOI: 10.1128/EC.4.3.577-587.2005, ISSN: 1535-9778.

Wolfe BA, Gould KL. Split decisions: coordinating cytokinesis in yeast. Trends Cell Biol. 2005 Jan; 15(1): 10-8. PMID: 15653073, PII: S0962-8924(04)00317-4, DOI: 10.1016/j.tcb.2004.11.006, ISSN: 0962-8924.

Ohi MD, Vander Kooi CW, Rosenberg JA, Ren L, Hirsch JP, Chazin WJ, Walz T, Gould KL. Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol. Cell. Biol. 2005 Jan; 25(1): 451-60. PMID: 15601865, PMCID: PMC538785, PII: 25/1/451, DOI: 10.1128/MCB.25.1.451-460.2005, ISSN: 0270-7306.

An H, Morrell JL, Jennings JL, Link AJ, Gould KL. Requirements of fission yeast septins for complex formation, localization, and function. Mol. Biol. Cell [print-electronic]. 2004 Dec; 15(12): 5551-64. PMID: 15385632, PMCID: PMC532033, PII: E04-07-0640, DOI: 10.1091/mbc.E04-07-0640, ISSN: 1059-1524.

Morrell JL, Nichols CB, Gould KL. The GIN4 family kinase, Cdr2p, acts independently of septins in fission yeast. J. Cell. Sci [print-electronic]. 2004 Oct 10/15/2004; 117(Pt 22): 5293-302. PMID: 15454577, PII: jcs.01409, DOI: 10.1242/jcs.01409, ISSN: 0021-9533.

Gould KL, Ren L, Feoktistova AS, Jennings JL, Link AJ. Tandem affinity purification and identification of protein complex components. Methods. 2004 Jul; 33(3): 239-44. PMID: 15157891, PII: S1046202303003189, DOI: 10.1016/j.ymeth.2003.11.019, ISSN: 1046-2023.

Gould KL. Protocols for experimentation with Schizosaccharomyces pombe [editorial]. Methods. 2004 Jul; 33(3): 187-8. PMID: 15157884, PII: S1046202303003116, DOI: 10.1016/j.ymeth.2003.11.012, ISSN: 1046-2023.

Venkatram S, Tasto JJ, Feoktistova A, Jennings JL, Link AJ, Gould KL. Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function. Mol. Biol. Cell [print-electronic]. 2004 May; 15(5): 2287-301. PMID: 15004232, PMCID: PMC404023, PII: E03-10-0728, DOI: 10.1091/mbc.E03-10-0728, ISSN: 1059-1524.

Wolfe BA, Gould KL. Inactivating Cdc25, mitotic style. Cell Cycle [print-electronic]. 2004 May; 3(5): 601-3. PMID: 15107615, PII: 891, ISSN: 1538-4101.

Morrell JL, Tomlin GC, Rajagopalan S, Venkatram S, Feoktistova AS, Tasto JJ, Mehta S, Jennings JL, Link A, Balasubramanian MK, Gould KL. Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB. Curr. Biol. 2004 Apr 4/6/2004; 14(7): 579-84. PMID: 15062098, PII: S096098220400209X, DOI: 10.1016/j.cub.2004.03.036, ISSN: 0960-9822.

Wolfe BA, Gould KL. Fission yeast Clp1p phosphatase affects G2/M transition and mitotic exit through Cdc25p inactivation. EMBO J [print-electronic]. 2004 Feb 2/25/2004; 23(4): 919-29. PMID: 14765109, PMCID: PMC381010, PII: 7600103, DOI: 10.1038/sj.emboj.7600103, ISSN: 0261-4189.

Carnahan RH, Gould KL. The PCH family protein, Cdc15p, recruits two F-actin nucleation pathways to coordinate cytokinetic actin ring formation in Schizosaccharomyces pombe. J. Cell Biol [print-electronic]. 2003 Sep 9/1/2003; 162(5): 851-62. PMID: 12939254, PMCID: PMC2172828, PII: jcb.200305012, DOI: 10.1083/jcb.200305012, ISSN: 0021-9525.

Zhang YZ, Cheng H, Gould KL, Golemis EA, Roder H. Structure, stability, and function of hDim1 investigated by NMR, circular dichroism, and mutational analysis. Biochemistry. 2003 Aug 8/19/2003; 42(32): 9609-18. PMID: 12911302, DOI: 10.1021/bi034486i, ISSN: 0006-2960.

Ohi MD, Vander Kooi CW, Rosenberg JA, Chazin WJ, Gould KL. Structural insights into the U-box, a domain associated with multi-ubiquitination. Nat. Struct. Biol. 2003 Apr; 10(4): 250-5. PMID: 12627222, PII: nsb906, DOI: 10.1038/nsb906, ISSN: 1072-8368.

Tasto JJ, Morrell JL, Gould KL. An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation. J. Cell Biol. 2003 Mar 3/31/2003; 160(7): 1093-103. PMID: 12668659, PMCID: PMC2172762, PII: jcb.200211126, DOI: 10.1083/jcb.200211126, ISSN: 0021-9525.

Yoon HJ, Feoktistova A, Wolfe BA, Jennings JL, Link AJ, Gould KL. Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr. Biol. 2002 Dec 12/10/2002; 12(23): 2048-54. PMID: 12477395, PII: S0960982202013313, ISSN: 0960-9822.

Guertin DA, Venkatram S, Gould KL, McCollum D. Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN). Dev. Cell. 2002 Dec; 3(6): 779-90. PMID: 12479804, PII: S1534580702003672, ISSN: 1534-5807.

Schadick K, Fourcade HM, Boumenot P, Seitz JJ, Morrell JL, Chang L, Gould KL, Partridge JF, Allshire RC, Kitagawa K, Hieter P, Hoffman CS. Schizosaccharomyces pombe Git7p, a member of the Saccharomyces cerevisiae Sgtlp family, is required for glucose and cyclic AMP signaling, cell wall integrity, and septation. Eukaryotic Cell. 2002 Aug; 1(4): 558-67. PMID: 12456004, PMCID: PMC118005, ISSN: 1535-9778.

MacCoss MJ, McDonald WH, Saraf A, Sadygov R, Clark JM, Tasto JJ, Gould KL, Wolters D, Washburn M, Weiss A, Clark JI, Yates JR. Shotgun identification of protein modifications from protein complexes and lens tissue. Proc. Natl. Acad. Sci. U.S.A. 2002 Jun 6/11/2002; 99(12): 7900-5. PMID: 12060738, PMCID: PMC122992, PII: 99/12/7900, DOI: 10.1073/pnas.122231399, ISSN: 0027-8424.

Ohi MD, Gould KL. Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA. 2002 Jun; 8(6): 798-815. PMID: 12088152, PMCID: PMC1370298, ISSN: 1355-8382.

Tomlin GC, Morrell JL, Gould KL. The spindle pole body protein Cdc11p links Sid4p to the fission yeast septation initiation network. Mol. Biol. Cell. 2002 Apr; 13(4): 1203-14. PMID: 11950932, PMCID: PMC102262, DOI: 10.1091/mbc.01-09-0455, ISSN: 1059-1524.

Ohi MD, Link AJ, Ren L, Jennings JL, McDonald WH, Gould KL. Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol. Cell. Biol. 2002 Apr; 22(7): 2011-24. PMID: 11884590, PMCID: PMC133674, ISSN: 0270-7306.

Burns CG, Ohi R, Mehta S, O'Toole ET, Winey M, Clark TA, Sugnet CW, Ares M, Gould KL. Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol. Cell. Biol. 2002 Feb; 22(3): 801-15. PMID: 11784857, PMCID: PMC133559, ISSN: 0270-7306.

Guertin, D.A., Venkatram, S., Gould, K.L., and McCollum, D. .. Dma1 prevents mitotic exit and cytokinesis by inhibiting the Septation Initiation Network (SIN). Dev. Cell. 2002; 3((8)): 779-90.

Chang L, Morrell JL, Feoktistova A, Gould KL. Study of cyclin proteolysis in anaphase-promoting complex (APC) mutant cells reveals the requirement for APC function in the final steps of the fission yeast septation initiation network. Mol. Cell. Biol. 2001 Oct; 21(19): 6681-94. PMID: 11533255, PMCID: PMC99813, ISSN: 0270-7306.

Feoktistova A, Magnelli P, Abeijon C, Perez P, Lester RL, Dickson RC, Gould KL. Coordination between fission yeast glucan formation and growth requires a sphingolipase activity. Genetics. 2001 Aug; 158(4): 1397-411. PMID: 11514435, PMCID: PMC1461765, ISSN: 0016-6731.

Ochotorena IL, Hirata D, Kominami K, Potashkin J, Sahin F, Wentz-Hunter K, Gould KL, Sato K, Yoshida Y, Vardy L, Toda T. Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation. J. Cell. Sci. 2001 Aug; 114(Pt 16): 2911-20. PMID: 11686295, ISSN: 0021-9533.

Trautmann S, Wolfe BA, Jorgensen P, Tyers M, Gould KL, McCollum D. Fission yeast Clp1p phosphatase regulates G2/M transition and coordination of cytokinesis with cell cycle progression. Curr. Biol. 2001 Jun 6/26/2001; 11(12): 931-40. PMID: 11448769, PII: S0960-9822(01)00268-8, ISSN: 0960-9822.

Tasto JJ, Carnahan RH, McDonald WH, Gould KL. Vectors and gene targeting modules for tandem affinity purification in Schizosaccharomyces pombe. Yeast. 2001 May; 18(7): 657-62. PMID: 11329175, DOI: 10.1002/yea.713, ISSN: 0749-503X.

McCollum D, Gould KL. Timing is everything: regulation of mitotic exit and cytokinesis by the MEN and SIN. Trends Cell Biol. 2001 Feb; 11(2): 89-95. PMID: 11166217, PII: S0962-8924(00)01901-2, ISSN: 0962-8924.

Behlke MA, Dames SA, McDonald WH, Gould KL, Devor EJ, Walder JA. Use of high specific activity StarFire oligonucleotide probes to visualize low-abundance pre-mRNA splicing intermediates in S. pombe. BioTechniques. 2000 Oct; 29(4): 892-7. PMID: 11056821, ISSN: 0736-6205.

Bishop DT, McDonald WH, Gould KL, Forsburg SL. Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosis. Nucleic Acids Res. 2000 Jun 6/1/2000; 28(11): 2214-20. PMID: 10871341, PMCID: PMC102626, ISSN: 1362-4962.

Chang L, Gould KL. Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 2000 May 5/9/2000; 97(10): 5249-54. PMID: 10805785, PMCID: PMC25814, PII: 97/10/5249, ISSN: 0027-8424.

Guertin DA, Chang L, Irshad F, Gould KL, McCollum D. The role of the sid1p kinase and cdc14p in regulating the onset of cytokinesis in fission yeast. EMBO J. 2000 Apr 4/17/2000; 19(8): 1803-15. PMID: 10775265, PMCID: PMC302011, DOI: 10.1093/emboj/19.8.1803, ISSN: 0261-4189.

Morrell JL, Morphew M, Gould KL. A mutant of Arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast. Mol. Biol. Cell. 1999 Dec; 10(12): 4201-15. PMID: 10588653, PMCID: PMC25753, ISSN: 1059-1524.

Burns CG, Ohi R, Krainer AR, Gould KL. Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Proc. Natl. Acad. Sci. U.S.A. 1999 Nov 11/23/1999; 96(24): 13789-94. PMID: 10570151, PMCID: PMC24143, ISSN: 0027-8424.

Zhang YZ, Gould KL, Dunbrack RL JR, Cheng H, Roder H, Golemis EA. The evolutionarily conserved Dim1 protein defines a novel branch of the thioredoxin fold superfamily. Physiol. Genomics. 1999 Nov 11/11/1999; 1(3): 109-18. PMID: 11015569, PII: 1/3/109, ISSN: 1531-2267.

McDonald WH, Ohi R, Smelkova N, Frendewey D, Gould KL. Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing. Mol. Cell. Biol. 1999 Aug; 19(8): 5352-62. PMID: 10409726, PMCID: PMC84378, ISSN: 0270-7306.

Feoktistova A, McCollum D, Ohi R, Gould KL. Identification and characterization of Schizosaccharomyces pombe asp1(+), a gene that interacts with mutations in the Arp2/3 complex and actin. Genetics. 1999 Jul; 152(3): 895-908. PMID: 10388810, PMCID: PMC1460656, ISSN: 0016-6731.

McCollum D, Feoktistova A, Gould KL. Phosphorylation of the myosin-II light chain does not regulate the timing of cytokinesis in fission yeast. J. Biol. Chem. 1999 Jun 6/18/1999; 274(25): 17691-5. PMID: 10364209, ISSN: 0021-9258.

Ohi R, Gould KL. Regulating the onset of mitosis. Curr. Opin. Cell Biol. 1999 Apr; 11(2): 267-73. PMID: 10209160, PII: S0955-0674(99)80036-2, ISSN: 0955-0674.

Berry LD, Feoktistova A, Wright MD, Gould KL. The schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component lid1(+) and is required for APC/C function. Mol. Cell. Biol. 1999 Apr; 19(4): 2535-46. PMID: 10082519, PMCID: PMC84046, ISSN: 0270-7306.

Naqvi NI, Eng K, Gould KL, Balasubramanian MK. Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast. EMBO J. 1999 Feb 2/15/1999; 18(4): 854-62. PMID: 10022828, PMCID: PMC1171178, DOI: 10.1093/emboj/18.4.854, ISSN: 0261-4189.

Machesky LM, Gould KL. The Arp2/3 complex: a multifunctional actin organizer. Curr. Opin. Cell Biol. 1999 Feb; 11(1): 117-21. PMID: 10047519, PII: S0955-0674(99)80014-3, ISSN: 0955-0674.

Burns CG, Gould KL. Connections between pre-mRNA processing and regulation of the eukaryotic cell cycle. Front Horm Res. 1999; 25: 59-82. PMID: 10941402, ISSN: 0301-3073.

Bähler J, Steever AB, Wheatley S, Wang Yl, Pringle JR, Gould KL, McCollum D. Role of polo kinase and Mid1p in determining the site of cell division in fission yeast. J. Cell Biol. 1998 Dec 12/14/1998; 143(6): 1603-16. PMID: 9852154, PMCID: PMC2132972, ISSN: 0021-9525.

Breeding CS, Hudson J, Balasubramanian MK, Hemmingsen SM, Young PG, Gould KL. The cdr2(+) gene encodes a regulator of G2/M progression and cytokinesis in Schizosaccharomyces pombe. Mol. Biol. Cell. 1998 Dec; 9(12): 3399-415. PMID: 9843577, PMCID: PMC25645, ISSN: 1059-1524.

Gould KL, Feoktistova A, Fleig U. A phosphorylation site mutant of Schizosaccharomyces pombe cdc2p fails to promote the metaphase to anaphase transition. Mol. Gen. Genet. 1998 Sep; 259(4): 437-48. PMID: 9790601, ISSN: 0026-8925.

Balasubramanian MK, McCollum D, Chang L, Wong KC, Naqvi NI, He X, Sazer S, Gould KL. Isolation and characterization of new fission yeast cytokinesis mutants. Genetics. 1998 Jul; 149(3): 1265-75. PMID: 9649519, PMCID: PMC1460233, ISSN: 0016-6731.

Gould KL, Burns CG, Feoktistova A, Hu CP, Pasion SG, Forsburg SL. Fission yeast cdc24(+) encodes a novel replication factor required for chromosome integrity. Genetics. 1998 Jul; 149(3): 1221-33. PMID: 9649516, PMCID: PMC1460225, ISSN: 0016-6731.

Ohi R, Feoktistova A, McCann S, Valentine V, Look AT, Lipsick JS, Gould KL. Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes. Mol. Cell. Biol. 1998 Jul; 18(7): 4097-108. PMID: 9632794, PMCID: PMC108994, ISSN: 0270-7306.

Gould KL, Simanis V. The control of septum formation in fission yeast. Genes Dev. 1997 Nov 11/15/1997; 11(22): 2939-51. PMID: 9367977, ISSN: 0890-9369.

Berry LD, Gould KL. Fission yeast dim1(+) encodes a functionally conserved polypeptide essential for mitosis. J. Cell Biol. 1997 Jun 6/16/1997; 137(6): 1337-54. PMID: 9182666, PMCID: PMC2132542, ISSN: 0021-9525.

Kemp JT, Balasubramanian MK, Gould KL. A wat1 mutant of fission yeast is defective in cell morphology. Mol. Gen. Genet. 1997 Mar 3/26/1997; 254(2): 127-38. PMID: 9108274, ISSN: 0026-8925.

Plyte SE, Feoktistova A, Burke JD, Woodgett JR, Gould KL. Schizosaccharomyces pombe skp1+ encodes a protein kinase related to mammalian glycogen synthase kinase 3 and complements a cdc14 cytokinesis mutant. Mol. Cell. Biol. 1997 Mar; 17(3): 1756. PMID: 9064503, PMCID: PMC231900, ISSN: 0270-7306.

Balasubramanian MK, McCollum D, Gould KL. Cytokinesis in fission yeast Schizosaccharomyces pombe. Meth. Enzymol. 1997; 283: 494-506. PMID: 9251043, PII: S0076-6879(97)83039-X, ISSN: 0076-6879.

McCollum D, Feoktistova A, Morphew M, Balasubramanian M, Gould KL. The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin. EMBO J. 1996 Dec 12/2/1996; 15(23): 6438-46. PMID: 8978671, PMCID: PMC452469, ISSN: 0261-4189.

Balasubramanian MK, Feoktistova A, McCollum D, Gould KL. Fission yeast Sop2p: a novel and evolutionarily conserved protein that interacts with Arp3p and modulates profilin function. EMBO J. 1996 Dec 12/2/1996; 15(23): 6426-37. PMID: 8978670, PMCID: PMC452468, ISSN: 0261-4189.

Ohi R, Feoktistova A, Gould KL. Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe. Gene. 1996 Oct 10/3/1996; 174(2): 315-8. PMID: 8890754, PII: 0378111996000856, ISSN: 0378-1119.

Gould KL, Feoktistova A. Characterization of novel mutations at the Schizosaccharomyces pombe cdc2 regulatory phosphorylation site, tyrosine 15. Mol. Biol. Cell. 1996 Oct; 7(10): 1573-86. PMID: 8898363, PMCID: PMC276007, ISSN: 1059-1524.

Berry LD, Gould KL. Novel alleles of cdc13 and cdc2 isolated as suppressors of mitotic catastrophe in Schizosaccharomyces pombe. Mol. Gen. Genet. 1996 Jul 7/26/1996; 251(6): 635-46. PMID: 8757394, ISSN: 0026-8925.

Berry LD, Gould KL. Regulation of Cdc2 activity by phosphorylation at T14/Y15. Prog Cell Cycle Res. 1996; 2: 99-105. PMID: 9552387, ISSN: 1087-2957.

Plyte SE, Feoktistova A, Burke JD, Woodgett JR, Gould KL. Schizosaccharomyces pombe skp1+ encodes a protein kinase related to mammalian glycogen synthase kinase 3 and complements a cdc14 cytokinesis mutant. Mol. Cell. Biol. 1996 Jan; 16(1): 179-91. PMID: 8524294, PMCID: PMC230991, ISSN: 0270-7306.

McCollum D, Balasubramanian MK, Pelcher LE, Hemmingsen SM, Gould KL. Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis. J. Cell Biol. 1995 Aug; 130(3): 651-60. PMID: 7622565, PMCID: PMC2120525, ISSN: 0021-9525.

Den Haese GJ, Walworth N, Carr AM, Gould KL. The Wee1 protein kinase regulates T14 phosphorylation of fission yeast Cdc2. Mol. Biol. Cell. 1995 Apr; 6(4): 371-85. PMID: 7626804, PMCID: PMC301198, ISSN: 1059-1524.

Balasubramanian MK, Hirani BR, Burke JD, Gould KL. The Schizosaccharomyces pombe cdc3+ gene encodes a profilin essential for cytokinesis. J. Cell Biol. 1994 Jun; 125(6): 1289-301. PMID: 8207058, PMCID: PMC2290924, ISSN: 0021-9525.

Ohi R, McCollum D, Hirani B, Den Haese GJ, Zhang X, Burke JD, Turner K, Gould KL. The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb. EMBO J. 1994 Jan 1/15/1994; 13(2): 471-83. PMID: 8313892, PMCID: PMC394831, ISSN: 0261-4189.

Burke JD, Gould KL. Molecular cloning and characterization of the Schizosaccharomyces pombe his3 gene for use as a selectable marker. Mol. Gen. Genet. 1994 Jan; 242(2): 169-76. PMID: 8159167, ISSN: 0026-8925.

Meddins AK, Nurse P, Gould KL. Analysis of 5' flanking sequences from the Schizosaccharomyces pombe cdc2 gene. Gene. 1993 May 5/15/1993; 127(1): 145-8. PMID: 8486281, PII: 0378-1119(93)90630-L, ISSN: 0378-1119.

Fleig UN, Gould KL, Nurse P. A dominant negative allele of p34cdc2 shows altered phosphoamino acid content and sequesters p56cdc13 cyclin. Mol. Cell. Biol. 1992 May; 12(5): 2295-301. PMID: 1533272, PMCID: PMC364401, ISSN: 0270-7306.

Gould KL, Moreno S, Owen DJ, Sazer S, Nurse P. Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function. EMBO J. 1991 Nov; 10(11): 3297-309. PMID: 1655416, PMCID: PMC453056, ISSN: 0261-4189.

Fleig UN, Gould KL. Regulation of cdc2 activity in Schizosaccharomyces pombe: the role of phosphorylation. Semin. Cell Biol. 1991 Aug; 2(4): 195-204. PMID: 1842338, ISSN: 1043-4682.

Enoch T, Gould KL, Nurse P. Mitotic checkpoint control in fission yeast. Cold Spring Harb. Symp. Quant. Biol. 1991; 56: 409-16. PMID: 1819501, ISSN: 0091-7451.

Gould KL, Moreno S, Tonks NK, Nurse P. Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase. Science. 1990 Dec 12/14/1990; 250(4987): 1573-6. PMID: 1703321, ISSN: 0036-8075.

Gould KL, Bretscher A, Esch FS, Hunter T. CDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1. EMBO J. 1989 Dec 12/20/1989; 8(13): 4133-42. PMID: 2591371, PMCID: PMC401598, ISSN: 0261-4189.

Gould KL, Nurse P. Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. Nature. 1989 Nov 11/2/1989; 342(6245): 39-45. PMID: 2682257, DOI: 10.1038/342039a0, ISSN: 0028-0836.

Gould KL, Hunter T. Platelet-derived growth factor induces multisite phosphorylation of pp60c-src and increases its protein-tyrosine kinase activity. Mol. Cell. Biol. 1988 Aug; 8(8): 3345-56. PMID: 2463476, PMCID: PMC363570, ISSN: 0270-7306.

Creutz CE, Zaks WJ, Hamman HC, Crane S, Martin WH, Gould KL, Oddie KM, Parsons SJ. Identification of chromaffin granule-binding proteins. Relationship of the chromobindins to calelectrin, synhibin, and the tyrosine kinase substrates p35 and p36. J. Biol. Chem. 1987 Feb 2/5/1987; 262(4): 1860-8. PMID: 2948960, ISSN: 0021-9258.

Woodgett JR, Gould KL, Hunter T. Substrate specificity of protein kinase C. Use of synthetic peptides corresponding to physiological sites as probes for substrate recognition requirements. Eur. J. Biochem. 1986 Nov 11/17/1986; 161(1): 177-84. PMID: 3023081, ISSN: 0014-2956.

Isacke CM, Meisenhelder J, Brown KD, Gould KL, Gould SJ, Hunter T. Early phosphorylation events following the treatment of Swiss 3T3 cells with bombesin and the mammalian bombesin-related peptide, gastrin-releasing peptide. EMBO J. 1986 Nov; 5(11): 2889-98. PMID: 2431903, PMCID: PMC1167239, ISSN: 0261-4189.

Gould KL, Woodgett JR, Isacke CM, Hunter T. The protein-tyrosine kinase substrate p36 is also a substrate for protein kinase C in vitro and in vivo. Mol. Cell. Biol. 1986 Jul; 6(7): 2738-44. PMID: 2946940, PMCID: PMC367834, ISSN: 0270-7306.

Gould KL, Cooper JA, Bretscher A, Hunter T. The protein-tyrosine kinase substrate, p81, is homologous to a chicken microvillar core protein. J. Cell Biol. 1986 Feb; 102(2): 660-9. PMID: 2418035, PMCID: PMC2114067, ISSN: 0021-9525.

Gould KL, Woodgett JR, Cooper JA, Buss JE, Shalloway D, Hunter T. Protein kinase C phosphorylates pp60src at a novel site. Cell. 1985 Oct; 42(3): 849-57. PMID: 2996780, PII: 0092-8674(85)90281-8, ISSN: 0092-8674.

Gould KL, Cooper JA, Hunter T. The 46,000-dalton tyrosine protein kinase substrate is widespread, whereas the 36,000-dalton substrate is only expressed at high levels in certain rodent tissues. J. Cell Biol. 1984 Feb; 98(2): 487-97. PMID: 6319429, PMCID: PMC2113118, ISSN: 0021-9525.

Available Postdoctoral Position Details
Posted: 8/21/2017

Applications are invited for Postdoctoral Fellows in the laboratory of Dr. Kathy Gould at Vanderbilt University School of Medicine in Nashville, Tennessee. The Gould Lab is interested in the mechanisms and regulation of cytokinesis. We use cutting edge approaches in proteomics/mass spectrometry, super-resolution imaging and live cell imaging, in combination with genetics and structural biology to answer mechanistic questions surrounding late cell cycle events.

Recently, we found that casein kinase 1 (CK1) family members mediate a cytokinesis checkpoint. CK1 family members have many functions, but the mechanisms regulating these enzymes remain unclear. We invite applications from postdoctoral scientists with expertise in structural biology to determine the structure of active CK1 enzymes. This feat has not been accomplished for any family member and will provide broadly relevant insight the CK1 family. The Vanderbilt Center for Structural Biology has state-of-the-art facilities for protein purification, crystallization, characterization, structural analysis and computation (http://structbio.vanderbilt.edu/).

Furthermore, we invite applications from postdoctoral scientists with a background in biochemistry and/or yeast genetics who share our interest in cytokinesis. We have projects available in all of our research areas. Successful applicants will have a proven ability to perform basic research in structural biology, biochemistry and/or yeast genetics, at least one first-author publication, and less than 3 years of relevant postdoctoral experience. To apply for a position, please send a cover letter, CV and the names and contact information for 3 references to Dr. Kathy Gould at kathy.gould@vanderbilt.edu. General information about being a postdoctoral fellow at Vanderbilt University can be obtained at https://medschool.vanderbilt.edu/postdoc/.