Claus Schneider, Ph.D.

Associate Professor

claus.schneider@vanderbilt.edu

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Faculty Appointments
Associate Professor of Pharmacology
Education
Ph.D., Food Chemistry, Universität Würzburg , Würzburg , GermanyB.A., Linguistics, Universität Würzburg , Würzburg , GermanyM.S., Food Chemistry, Universität Würzburg , Würzburg , Germany
Office Address
514 RRB
TN 6602
Research Description
We are studying biotransformations of small molecules in vitro and in vivo in the context of human health and disease. We determine the structures of novel metabolites, the enzymes and reaction mechanisms involved in their formation, and quantify their abundance in vivo in animals and humans. We also investigate the biological activity of the newly identified metabolites using cell culture and animal models.

We are currently interested in two groups of small molecule compounds, namely eicosanoids and polyphenols. Eicosanoids, including prostaglandins and leukotrienes, are derived from the ¿-6 polyunsaturated fatty acid, arachidonic acid. Polyphenols are bioactive compounds derived from plants and considered the major ingredients responsible for the health benefits of fruits and vegetables.

We are working on several specific projects:

1. Discovery of novel eicosanoids
Two major enzymes involved in the biosynthesis of eicosanoids are 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2), forming leukotrienes and prostaglandins, respectively. Leukotrienes and prostaglandins are the targets of widely used anti-asthma and anti-inflammatory/analgesic drugs. We have discovered a biosynthetic cross-over of the 5-LOX and COX-2 enzymes, resulting in the formation of novel eicosanoids that we have identified and named hemiketals (HK) HKE2 and HKD2. We are now studying the formation and role of HKE2 and HKD2 in animal and cellular models of inflammation.

2. Metabolism of eicosanoids
Prostaglandins are important lipid mediators but their abundance is difficult to quantify directly in blood or tissues. Therefore, the established approach is the quantification of their urinary metabolites. We are analyzing novel biochemical transformations involved in the metabolism of prostaglandins using purified enzymes and cultured cells but also animals and humans. Our studies have important ramifications for understanding the action of, for example, the cardioprotective activity of aspirin.

3. Biological activity of curcumin and other polyphenols
Our favorite polyphenol is curcumin, the yellow-orange ingredient of the spice curry. Curcumin has been used as an anti-inflammatory agent in traditional Asian medicine for centuries. When studying the degradation of curcumin (a rapid reaction in buffer), we discovered a large number of novel metabolites and identified their chemical structure and mechanism of formation. We are now testing the hypothesis that unstable reaction intermediates formed in the degradation of curcumin mediate the biological activity by binding to specific protein targets.

The lab uses HPLC and LC-MS techniques for analysis and quantification of metabolites. We rely on NMR for the structural identification of novel products. We use cell culture and animal models and also proteomics approaches to investigate the biological activities of novel metabolites that we discover. We maintain several active protocols to carry out studies in animals and humans.


Research Keywords
Discovery and characterization of novel lipid mediators. Mechanisms of oxygenation of fatty acids. Chemopreventive and anti-inflammatory activity of curcumin. Additional keywords: HPLC, LC-MS, NMR, reaction mechanism, quantification, isotope standards, leukocytes, chemical synthesis, inflammation, platelets
Publications
Kunihiro AG, Luis PB, Brickey JA, Frye JB, Chow HS, Schneider C, Funk JL. Beta-Glucuronidase Catalyzes Deconjugation and Activation of Curcumin-Glucuronide in Bone. J. Nat. Prod [print-electronic]. 2019 Mar 3/22/2019; 82(3): 500-9. PMID: 30794412, PMCID: PMC6528680, DOI: 10.1021/acs.jnatprod.8b00873, ISSN: 1520-6025.

Sierra JC, Suarez G, Piazuelo MB, Luis PB, Baker DR, Romero-Gallo J, Barry DP, Schneider C, Morgan DR, Peek RM, Gobert AP, Wilson KT. A-Difluoromethylornithine reduces gastric carcinogenesis by causing mutations in Helicobacter pylori cagY. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2019 Mar 3/12/2019; 116(11): 5077-85. PMID: 30804204, PMCID: PMC6421409, PII: 1814497116, DOI: 10.1073/pnas.1814497116, ISSN: 1091-6490.

Kunihiro AG, Brickey JA, Frye JB, Luis PB, Schneider C, Funk JL. Curcumin, but not curcumin-glucuronide, inhibits Smad signaling in TGFß-dependent bone metastatic breast cancer cells and is enriched in bone compared to other tissues. J. Nutr. Biochem [print-electronic]. 2019 Jan; 63: 150-6. PMID: 30393127, PMCID: PMC6296872, PII: S0955-2863(18)30470-4, DOI: 10.1016/j.jnutbio.2018.09.021, ISSN: 1873-4847.

Joseph AI, Luis PB, Schneider C. A Curcumin Degradation Product, 7-Norcyclopentadione, Formed by Aryl Migration and Loss of a Carbon from the Heptadienedione Chain. J. Nat. Prod [print-electronic]. 2018 Dec 12/28/2018; 81(12): 2756-62. PMID: 30560664, PMCID: PMC6474840, DOI: 10.1021/acs.jnatprod.8b00822, ISSN: 1520-6025.

Giménez-Bastida JA, Boeglin WE, Boutaud O, Malkowski MG, Schneider C. Residual cyclooxygenase activity of aspirin-acetylated COX-2 forms 15 R-prostaglandins that inhibit platelet aggregation. FASEB J [print-electronic]. 2018 Aug 8/10/2018; fj201801018R. PMID: 30096040, DOI: 10.1096/fj.201801018R, ISSN: 1530-6860.

Singh K, Coburn LA, Asim M, Barry DP, Allaman MM, Shi C, Washington MK, Luis PB, Schneider C, Delgado AG, Piazuelo MB, Cleveland JL, Gobert AP, Wilson KT. Ornithine Decarboxylase in Macrophages Exacerbates Colitis and Promotes Colitis-Associated Colon Carcinogenesis by Impairing M1 Immune Responses. Cancer Res [print-electronic]. 2018 Aug 8/1/2018; 78(15): 4303-15. PMID: 29853605, PMCID: PMC6072585, PII: 0008-5472.CAN-18-0116, DOI: 10.1158/0008-5472.CAN-18-0116, ISSN: 1538-7445.

Boer RE, Giménez-Bastida JA, Boutaud O, Jana S, Schneider C, Sulikowski GA. Total Synthesis and Biological Activity of the Arachidonic Acid Metabolite Hemiketal E2. Org. Lett [print-electronic]. 2018 Jul 7/6/2018; 20(13): 4020-2. PMID: 29916257, DOI: 10.1021/acs.orglett.8b01578, ISSN: 1523-7052.

Skiba MB, Luis PB, Alfafara C, Billheimer D, Schneider C, Funk JL. Curcuminoid Content and Safety-Related Markers of Quality of Turmeric Dietary Supplements Sold in an Urban Retail Marketplace in the United States. Mol Nutr Food Res [print-electronic]. 2018 May 5/29/2018; e1800143. PMID: 29808963, DOI: 10.1002/mnfr.201800143, ISSN: 1613-4133.

Joseph AI, Edwards RL, Luis PB, Presley SH, Porter NA, Schneider C. Stability and anti-inflammatory activity of the reduction-resistant curcumin analog, 2,6-dimethyl-curcumin. Org. Biomol. Chem. 2018 May 5/2/2018; 16(17): 3273-81. PMID: 29664496, PMCID: PMC5932260, DOI: 10.1039/c8ob00639c, ISSN: 1477-0539.

Luis PB, Boeglin WE, Schneider C. Thiol Reactivity of Curcumin and Its Oxidation Products. Chem. Res. Toxicol [print-electronic]. 2018 Apr 4/16/2018; 31(4): 269-76. PMID: 29569909, DOI: 10.1021/acs.chemrestox.7b00326, ISSN: 1520-5010.

Nakashima F, Shibata T, Kamiya K, Yoshitake J, Kikuchi R, Matsushita T, Ishii I, Giménez-Bastida JA, Schneider C, Uchida K. Structural and functional insights into S-thiolation of human serum albumins. Sci Rep. 2018 Jan 1/17/2018; 8(1): 932. PMID: 29343798, PMCID: PMC5772555, PII: 10.1038/s41598-018-19610-9, DOI: 10.1038/s41598-018-19610-9, ISSN: 2045-2322.

Gobert AP, Al-Greene NT, Singh K, Coburn LA, Sierra JC, Verriere TG, Luis PB, Schneider C, Asim M, Allaman MM, Barry DP, Cleveland JL, Destefano Shields CE, Casero RA, Washington MK, Piazuelo MB, Wilson KT. Distinct Immunomodulatory Effects of Spermine Oxidase in Colitis Induced by Epithelial Injury or Infection. Front Immunol. 2018; 9: 1242. PMID: 29922289, PMCID: PMC5996034, DOI: 10.3389/fimmu.2018.01242, ISSN: 1664-3224.

Humpf HU, Schneider C, Stevens JF, Mayer C. MNF's Upcoming Topics, Structure, and Standards in 2018 [editorial]. Mol Nutr Food Res. 2018 Jan; 62(1): PMID: 29319238, DOI: 10.1002/mnfr.201870014, ISSN: 1613-4133.

Edwards RL, Luis PB, Varuzza PV, Joseph AI, Presley SH, Chaturvedi R, Schneider C. The anti-inflammatory activity of curcumin is mediated by its oxidative metabolites. J. Biol. Chem [print-electronic]. 2017 Dec 12/29/2017; 292(52): 21243-52. PMID: 29097552, PMCID: PMC5766941, PII: RA117.000123, DOI: 10.1074/jbc.RA117.000123, ISSN: 1083-351X.

Fernández-Del-Río L, Nag A, Gutiérrez Casado E, Ariza J, Awad AM, Joseph AI, Kwon O, Verdin E, de Cabo R, Schneider C, Torres JZ, Burón MI, Clarke CF, Villalba JM. Kaempferol increases levels of coenzyme Q in kidney cells and serves as a biosynthetic ring precursor. Free Radic. Biol. Med [print-electronic]. 2017 Sep; 110: 176-87. PMID: 28603085, PMCID: PMC5539908, PII: S0891-5849(17)30629-9, DOI: 10.1016/j.freeradbiomed.2017.06.006, ISSN: 1873-4596.

Teder T, Boeglin WE, Schneider C, Brash AR. A fungal catalase reacts selectively with the 13S fatty acid hydroperoxide products of the adjacent lipoxygenase gene and exhibits 13S-hydroperoxide-dependent peroxidase activity. Biochim. Biophys. Acta [print-electronic]. 2017 Jul; 1862(7): 706-15. PMID: 28363790, PMCID: PMC5481161, PII: S1388-1981(17)30059-8, DOI: 10.1016/j.bbalip.2017.03.011, ISSN: 0006-3002.

Luis PB, Gordon ON, Nakashima F, Joseph AI, Shibata T, Uchida K, Schneider C. Oxidative metabolism of curcumin-glucuronide by peroxidases and isolated human leukocytes. Biochem. Pharmacol [print-electronic]. 2017 May 5/15/2017; 132: 143-9. PMID: 28274615, PMCID: PMC5390522, PII: S0006-2952(17)30117-X, DOI: 10.1016/j.bcp.2017.03.002, ISSN: 1873-2968.

Giménez-Bastida JA, Shibata T, Uchida K, Schneider C. Roles of 5-lipoxygenase and cyclooxygenase-2 in the biosynthesis of hemiketals E2 and D2 by activated human leukocytes. FASEB J [print-electronic]. 2017 May; 31(5): 1867-78. PMID: 28096231, PMCID: PMC5388546, PII: fj.201601136R, DOI: 10.1096/fj.201601136R, ISSN: 1530-6860.

Lu J, Guo S, Xue X, Chen Q, Ge J, Zhuo Y, Zhong H, Chen B, Zhao M, Han W, Suzuki T, Zhu M, Xia L, Schneider C, Blackwell TS, Porter NA, Zheng L, Tsimikas S, Yin H. Identification of a novel series of anti-inflammatory and anti-oxidative phospholipid oxidation products containing the cyclopentenone moiety in vitro and in vivo: Implication in atherosclerosis. J. Biol. Chem [print-electronic]. 2017 Mar 3/31/2017; 292(13): 5378-91. PMID: 28202546, PMCID: PMC5392682, PII: M116.751909, DOI: 10.1074/jbc.M116.751909, ISSN: 1083-351X.

Hardbower DM, Asim M, Luis PB, Singh K, Barry DP, Yang C, Steeves MA, Cleveland JL, Schneider C, Piazuelo MB, Gobert AP, Wilson KT. Ornithine decarboxylase regulates M1 macrophage activation and mucosal inflammation via histone modifications. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2017 Jan 1/31/2017; 114(5): E751-E760. PMID: 28096401, PMCID: PMC5293075, PII: 1614958114, DOI: 10.1073/pnas.1614958114, ISSN: 1091-6490.

Giménez-Bastida JA, Suzuki T, Sprinkel KC, Boeglin WE, Schneider C. Biomimetic synthesis of hemiketal eicosanoids for biological testing. Prostaglandins Other Lipid Mediat [print-electronic]. 2016 Sep 9/3/2016; PMID: 27596332, PMCID: PMC5336528, PII: S1098-8823(16)30107-1, DOI: 10.1016/j.prostaglandins.2016.09.001, ISSN: 1098-8823.

Schneider C, Gordon ON, Edwards RL, Luis PB. Degradation of Curcumin: From Mechanism to Biological Implications. J. Agric. Food Chem [print-electronic]. 2015 Sep 9/9/2015; 63(35): 7606-14. PMID: 25817068, PMCID: PMC4752206, DOI: 10.1021/acs.jafc.5b00244, ISSN: 1520-5118.

Gordon ON, Luis PB, Ashley RE, Osheroff N, Schneider C. Oxidative Transformation of Demethoxy- and Bisdemethoxycurcumin: Products, Mechanism of Formation, and Poisoning of Human Topoisomerase IIa. Chem. Res. Toxicol [print-electronic]. 2015 May 5/18/2015; 28(5): 989-96. PMID: 25806475, PMCID: PMC4437832, DOI: 10.1021/acs.chemrestox.5b00009, ISSN: 1520-5010.

Gordon ON, Luis PB, Sintim HO, Schneider C. Unraveling curcumin degradation: autoxidation proceeds through spiroepoxide and vinylether intermediates en route to the main bicyclopentadione. J. Biol. Chem [print-electronic]. 2015 Feb 2/20/2015; 290(8): 4817-28. PMID: 25564617, PMCID: PMC4335222, PII: M114.618785, DOI: 10.1074/jbc.M114.618785, ISSN: 1083-351X.

Orlando BJ, McDougle DR, Lucido MJ, Eng ET, Graham LA, Schneider C, Stokes DL, Das A, Malkowski MG. Cyclooxygenase-2 catalysis and inhibition in lipid bilayer nanodiscs. Arch. Biochem. Biophys [print-electronic]. 2014 Mar 3/15/2014; 546: 33-40. PMID: 24503478, PMCID: PMC3967765, PII: S0003-9861(14)00053-8, DOI: 10.1016/j.abb.2014.01.026, ISSN: 1096-0384.

Gordon ON, Graham LA, Schneider C. Facile synthesis of deuterated and [(14) C]labeled analogs of vanillin and curcumin for use as mechanistic and analytical tools. J Labelled Comp Radiopharm [print-electronic]. 2013 Dec; 56(14): 696-9. PMID: 24339007, PMCID: PMC3943166, DOI: 10.1002/jlcr.3102, ISSN: 1099-1344.

Brash AR, Boeglin WE, Stec DF, Voehler M, Schneider C, Cha JK. Isolation and characterization of two geometric allene oxide isomers synthesized from 9S-hydroperoxylinoleic acid by cytochrome P450 CYP74C3: stereochemical assignment of natural fatty acid allene oxides. J. Biol. Chem [print-electronic]. 2013 Jul 7/19/2013; 288(29): 20797-806. PMID: 23709224, PMCID: PMC3774351, PII: M113.482521, DOI: 10.1074/jbc.M113.482521, ISSN: 1083-351X.

Ketron AC, Gordon ON, Schneider C, Osheroff N. Oxidative metabolites of curcumin poison human type II topoisomerases. Biochemistry [print-electronic]. 2013 Jan 1/8/2013; 52(1): 221-7. PMID: 23253398, PMCID: PMC3541001, DOI: 10.1021/bi3014455, ISSN: 1520-4995.

Brash AR, Schneider C, Hamberg M. Applications of stereospecifically-labeled Fatty acids in oxygenase and desaturase biochemistry. Lipids [print-electronic]. 2012 Feb; 47(2): 101-16. PMID: 21971646, PMCID: PMC3315059, DOI: 10.1007/s11745-011-3612-7, ISSN: 1558-9307.

Gordon, ON, Schneider, C. Vanillin and ferulic acid: not the major degradation products of curcumin. 2012.

Tejera N, Boeglin WE, Suzuki T, Schneider C. COX-2-dependent and -independent biosynthesis of dihydroxy-arachidonic acids in activated human leukocytes. J. Lipid Res [print-electronic]. 2012 Jan; 53(1): 87-94. PMID: 22068350, PMCID: PMC3243484, PII: jlr.M017822, DOI: 10.1194/jlr.M017822, ISSN: 0022-2275.

McElroy SJ, Hobbs S, Kallen M, Tejera N, Rosen MJ, Grishin A, Matta P, Schneider C, Upperman J, Ford H, Polk DB, Weitkamp JH. Transactivation of EGFR by LPS induces COX-2 expression in enterocytes. PLoS ONE [print-electronic]. 2012; 7(5): e38373. PMID: 22675459, PMCID: PMC3364993, PII: PONE-D-11-25419, DOI: 10.1371/journal.pone.0038373, ISSN: 1932-6203.

Schneider C, Pozzi A. Cyclooxygenases and lipoxygenases in cancer. Cancer Metastasis Rev. 2011 Dec; 30(3-4): 277-94. PMID: 22002716, PMCID: PMC3798028, DOI: 10.1007/s10555-011-9310-3, ISSN: 1573-7233.

Lee SH, Schneider C, Higdon AN, Darley-Usmar VM, Chung CY. Role of iPLA(2) in the regulation of Src trafficking and microglia chemotaxis. Traffic [print-electronic]. 2011 Jul; 12(7): 878-89. PMID: 21438970, PMCID: PMC3115410, DOI: 10.1111/j.1600-0854.2011.01195.x, ISSN: 1600-0854.

Griesser M, Suzuki T, Tejera N, Mont S, Boeglin WE, Pozzi A, Schneider C. Biosynthesis of hemiketal eicosanoids by cross-over of the 5-lipoxygenase and cyclooxygenase-2 pathways. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2011 Apr 4/26/2011; 108(17): 6945-50. PMID: 21482803, PMCID: PMC3084063, PII: 1019473108, DOI: 10.1073/pnas.1019473108, ISSN: 1091-6490.

Griesser M, Pistis V, Suzuki T, Tejera N, Pratt DA, Schneider C. Autoxidative and cyclooxygenase-2 catalyzed transformation of the dietary chemopreventive agent curcumin. J. Biol. Chem [print-electronic]. 2011 Jan 1/14/2011; 286(2): 1114-24. PMID: 21071447, PMCID: PMC3020718, PII: M110.178806, DOI: 10.1074/jbc.M110.178806, ISSN: 1083-351X.

Liu W, Porter NA, Schneider C, Brash AR, Yin H. Formation of 4-hydroxynonenal from cardiolipin oxidation: Intramolecular peroxyl radical addition and decomposition. Free Radic. Biol. Med [print-electronic]. 2011 Jan 1/1/2011; 50(1): 166-78. PMID: 21047551, PMCID: PMC3014443, PII: S0891-5849(10)01348-1, DOI: 10.1016/j.freeradbiomed.2010.10.709, ISSN: 1873-4596.

Mulugeta S, Suzuki T, Hernandez NT, Griesser M, Boeglin WE, Schneider C. Identification and absolute configuration of dihydroxy-arachidonic acids formed by oxygenation of 5S-HETE by native and aspirin-acetylated COX-2. J. Lipid Res [print-electronic]. 2010 Mar; 51(3): 575-85. PMID: 19752399, PMCID: PMC2817587, PII: jlr.M001719, DOI: 10.1194/jlr.M001719, ISSN: 0022-2275.

Griesser M, Boeglin WE, Suzuki T, Schneider C. Convergence of the 5-LOX and COX-2 pathways: heme-catalyzed cleavage of the 5S-HETE-derived di-endoperoxide into aldehyde fragments. J. Lipid Res [print-electronic]. 2009 Dec; 50(12): 2455-62. PMID: 19553698, PMCID: PMC2781317, PII: M900181-JLR200, DOI: 10.1194/jlr.M900181-JLR200, ISSN: 0022-2275.

Gao B, Boeglin WE, Zheng Y, Schneider C, Brash AR. Evidence for an ionic intermediate in the transformation of fatty acid hydroperoxide by a catalase-related allene oxide synthase from the Cyanobacterium Acaryochloris marina. J. Biol. Chem [print-electronic]. 2009 Aug 8/14/2009; 284(33): 22087-98. PMID: 19531485, PMCID: PMC2755933, PII: M109.013151, DOI: 10.1074/jbc.M109.013151, ISSN: 0021-9258.

Niisuke K, Boeglin WE, Murray JJ, Schneider C, Brash AR. Biosynthesis of a linoleic acid allylic epoxide: mechanistic comparison with its chemical synthesis and leukotriene A biosynthesis. J. Lipid Res [print-electronic]. 2009 Jul; 50(7): 1448-55. PMID: 19244216, PMCID: PMC2694342, PII: M900025-JLR200, DOI: 10.1194/jlr.M900025-JLR200, ISSN: 0022-2275.

Schneider C. An update on products and mechanisms of lipid peroxidation. Mol Nutr Food Res. 2009 Mar; 53(3): 315-21. PMID: 19006094, PMCID: PMC2868362, DOI: 10.1002/mnfr.200800131, ISSN: 1613-4133.

Schneider C, Porter NA, Brash AR. Routes to 4-hydroxynonenal: fundamental issues in the mechanisms of lipid peroxidation. J. Biol. Chem [print-electronic]. 2008 Jun 6/6/2008; 283(23): 15539-43. PMID: 18285327, PMCID: PMC2414272, PII: R800001200, DOI: 10.1074/jbc.R800001200, ISSN: 0021-9258.

Schneider C, Boeglin WE, Yin H, Porter NA, Brash AR. Intermolecular peroxyl radical reactions during autoxidation of hydroxy and hydroperoxy arachidonic acids generate a novel series of epoxidized products. Chem. Res. Toxicol [print-electronic]. 2008 Apr; 21(4): 895-903. PMID: 18324788, DOI: 10.1021/tx700357u, ISSN: 0893-228X.

Zheng Y, Boeglin WE, Schneider C, Brash AR. A 49-kDa mini-lipoxygenase from Anabaena sp. PCC 7120 retains catalytically complete functionality. J. Biol. Chem [print-electronic]. 2008 Feb 2/22/2008; 283(8): 5138-47. PMID: 18070874, PII: M705780200, DOI: 10.1074/jbc.M705780200, ISSN: 0021-9258.

Schneider C, Niisuke K, Boeglin WE, Voehler M, Stec DF, Porter NA, Brash AR. Enzymatic synthesis of a bicyclobutane fatty acid by a hemoprotein lipoxygenase fusion protein from the cyanobacterium Anabaena PCC 7120. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2007 Nov 11/27/2007; 104(48): 18941-5. PMID: 18025466, PMCID: PMC2141887, PII: 0707148104, DOI: 10.1073/pnas.0707148104, ISSN: 1091-6490.

Brash AR, Yu Z, Boeglin WE, Schneider C. The hepoxilin connection in the epidermis. FEBS J [print-electronic]. 2007 Jul; 274(14): 3494-502. PMID: 17608720, PII: EJB5909, DOI: 10.1111/j.1742-4658.2007.05909.x, ISSN: 1742-464X.

Yu Z, Schneider C, Boeglin WE, Brash AR. Epidermal lipoxygenase products of the hepoxilin pathway selectively activate the nuclear receptor PPARalpha. Lipids [print-electronic]. 2007 Jun; 42(6): 491-7. PMID: 17436029, DOI: 10.1007/s11745-007-3054-4, ISSN: 0024-4201.

Schneider C, Pratt DA, Porter NA, Brash AR. Control of oxygenation in lipoxygenase and cyclooxygenase catalysis. Chem. Biol. 2007 May; 14(5): 473-88. PMID: 17524979, PMCID: PMC2692746, PII: S1074-5521(07)00145-7, DOI: 10.1016/j.chembiol.2007.04.007, ISSN: 1074-5521.

Schneider C, Yu Z, Boeglin WE, Zheng Y, Brash AR. Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatography. Meth. Enzymol. 2007; 433: 145-57. PMID: 17954233, PII: S0076-6879(07)33008-5, DOI: 10.1016/S0076-6879(07)33008-5, ISSN: 0076-6879.

Yu Z, Schneider C, Boeglin WE, Brash AR. Human and mouse eLOX3 have distinct substrate specificities: implications for their linkage with lipoxygenases in skin. Arch. Biochem. Biophys [print-electronic]. 2006 Nov 11/15/2006; 455(2): 188-96. PMID: 17045234, PMCID: PMC2636205, PII: S0003-9861(06)00331-6, DOI: 10.1016/j.abb.2006.09.002, ISSN: 0003-9861.

Furse KE, Pratt DA, Schneider C, Brash AR, Porter NA, Lybrand TP. Molecular dynamics simulations of arachidonic acid-derived pentadienyl radical intermediate complexes with COX-1 and COX-2: insights into oxygenation regio- and stereoselectivity. Biochemistry. 2006 Mar 3/14/2006; 45(10): 3206-18. PMID: 16519515, PMCID: PMC2504531, DOI: 10.1021/bi052338h, ISSN: 0006-2960.

Schneider C, Boeglin WE, Yin H, Stec DF, Voehler M. Convergent oxygenation of arachidonic acid by 5-lipoxygenase and cyclooxygenase-2. J. Am. Chem. Soc. 2006 Jan 1/25/2006; 128(3): 720-1. PMID: 16417352, PMCID: PMC2532595, DOI: 10.1021/ja056517y, ISSN: 0002-7863.

Coffa G, Schneider C, Brash AR. A comprehensive model of positional and stereo control in lipoxygenases. Biochem. Biophys. Res. Commun [print-electronic]. 2005 Dec 12/9/2005; 338(1): 87-92. PMID: 16111652, PII: S0006-291X(05)01688-8, DOI: 10.1016/j.bbrc.2005.07.185, ISSN: 0006-291X.

Coffa G, Imber AN, Maguire BC, Laxmikanthan G, Schneider C, Gaffney BJ, Brash AR. On the relationships of substrate orientation, hydrogen abstraction, and product stereochemistry in single and double dioxygenations by soybean lipoxygenase-1 and its Ala542Gly mutant. J. Biol. Chem [print-electronic]. 2005 Nov 11/18/2005; 280(46): 38756-66. PMID: 16157595, PMCID: PMC1351262, PII: M504870200, DOI: 10.1074/jbc.M504870200, ISSN: 0021-9258.

Schneider C, Boeglin WE, Yin H, Ste DF, Hachey DL, Porter NA, Brash AR. Synthesis of dihydroperoxides of linoleic and linolenic acids and studies on their transformation to 4-hydroperoxynonenal. Lipids. 2005 Nov; 40(11): 1155-62. PMID: 16459928, ISSN: 0024-4201.

Chang MS, Schneider C, Roberts RL, Shappell SB, Haselton FR, Boeglin WE, Brash AR. Detection and subcellular localization of two 15S-lipoxygenases in human cornea. Invest. Ophthalmol. Vis. Sci. 2005 Mar; 46(3): 849-56. PMID: 15728540, PII: 46/3/849, DOI: 10.1167/iovs.04-1166, ISSN: 0146-0404.

Yu Z, Schneider C, Boeglin WE, Brash AR. Mutations associated with a congenital form of ichthyosis (NCIE) inactivate the epidermal lipoxygenases 12R-LOX and eLOX3. Biochim. Biophys. Acta. 2005 Jan 1/5/2005; 1686(3): 238-47. PMID: 15629692, PII: S1388-1981(04)00184-2, DOI: 10.1016/j.bbalip.2004.10.007, ISSN: 0006-3002.

Schneider C, Boeglin WE, Brash AR. Human cyclo-oxygenase-1 and an alternative splice variant: contrasts in expression of mRNA, protein and catalytic activities. Biochem. J. 2005 Jan 1/1/2005; 385(Pt 1): 57-64. PMID: 15361066, PMCID: PMC1134673, PII: BJ20041115, DOI: 10.1042/BJ20041115, ISSN: 1470-8728.

Schneider C. Chemistry and biology of vitamin E. Mol Nutr Food Res. 2005 Jan; 49(1): 7-30. PMID: 15580660, DOI: 10.1002/mnfr.200400049, ISSN: 1613-4125.

Valmsen K, Boeglin WE, Järving I, Schneider C, Varvas K, Brash AR, Samel N. Structural and functional comparison of 15S- and 15R-specific cyclooxygenases from the coral Plexaura homomalla. Eur. J. Biochem. 2004 Sep; 271(17): 3533-8. PMID: 15317588, PII: EJB4289, DOI: 10.1111/j.0014-2956.2004.04289.x, ISSN: 0014-2956.

Schneider C, Porter NA, Brash AR. Autoxidative transformation of chiral omega6 hydroxy linoleic and arachidonic acids to chiral 4-hydroxy-2E-nonenal. Chem. Res. Toxicol. 2004 Jul; 17(7): 937-41. PMID: 15257619, DOI: 10.1021/tx049913n, ISSN: 0893-228X.

West JD, Ji C, Duncan ST, Amarnath V, Schneider C, Rizzo CJ, Brash AR, Marnett LJ. Induction of apoptosis in colorectal carcinoma cells treated with 4-hydroxy-2-nonenal and structurally related aldehydic products of lipid peroxidation. Chem. Res. Toxicol. 2004 Apr; 17(4): 453-62. PMID: 15089087, DOI: 10.1021/tx034248o, ISSN: 0893-228X.

Schneider C, Strayhorn WD, Brantley DM, Nanney LB, Yull FE, Brash AR. Upregulation of 8-lipoxygenase in the dermatitis of IkappaB-alpha-deficient mice. J. Invest. Dermatol. 2004 Mar; 122(3): 691-8. PMID: 15086555, PII: JID22329, DOI: 10.1111/j.0022-202X.2004.22329.x, ISSN: 0022-202X.

Schneider C, Boeglin WE, Brash AR. Identification of two cyclooxygenase active site residues, Leucine 384 and Glycine 526, that control carbon ring cyclization in prostaglandin biosynthesis. J. Biol. Chem [print-electronic]. 2004 Feb 2/6/2004; 279(6): 4404-14. PMID: 14594816, PII: M307431200, DOI: 10.1074/jbc.M307431200, ISSN: 0021-9258.

Yu Z, Schneider C, Boeglin WE, Marnett LJ, Brash AR. The lipoxygenase gene ALOXE3 implicated in skin differentiation encodes a hydroperoxide isomerase. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2003 Aug 8/5/2003; 100(16): 9162-7. PMID: 12881489, PMCID: PMC170889, PII: 1633612100, DOI: 10.1073/pnas.1633612100, ISSN: 0027-8424.

Brash, Alan R., Jisaka, Mitsuo, Boeglin, William E., Schneider, Claus. On the basis for the positional specificity and stereo specificity of lipoxygenases. Int. Congress Series. 2003; 1233: 297-305.

Schneider C, Brash AR. Lipoxygenase-catalyzed formation of R-configuration hydroperoxides. Prostaglandins Other Lipid Mediat. 2002 Aug; 68-69: 291-301. PMID: 12432924, ISSN: 1098-8823.

Schneider C, Manier ML, Hachey DL, Brash AR. Detection of the 15-acetate of prostaglandin E2 methyl ester as a prominent component of the prostaglandins in the gorgonian coral Plexaura homomalla. Lipids. 2002 Feb; 37(2): 217-21. PMID: 11908914, ISSN: 0024-4201.

Schneider C, Boeglin WE, Prusakiewicz JJ, Rowlinson SW, Marnett LJ, Samel N, Brash AR. Control of prostaglandin stereochemistry at the 15-carbon by cyclooxygenases-1 and -2. A critical role for serine 530 and valine 349. J. Biol. Chem [print-electronic]. 2002 Jan 1/4/2002; 277(1): 478-85. PMID: 11677234, PII: M107471200, DOI: 10.1074/jbc.M107471200, ISSN: 0021-9258.

Schneider C, Boeglin WE, Brash AR. Analysis of cyclooxygenase-substrate interactions using stereospecifically-labeled arachidonic acids. Adv. Exp. Med. Biol. 2002; 507: 49-53. PMID: 12664563, ISSN: 0065-2598.

Kozak KR, Prusakiewicz JJ, Rowlinson SW, Schneider C, Marnett LJ. Amino acid determinants in cyclooxygenase-2 oxygenation of the endocannabinoid 2-arachidonylglycerol. J. Biol. Chem [print-electronic]. 2001 Aug 8/10/2001; 276(32): 30072-7. PMID: 11402053, PII: M104467200, DOI: 10.1074/jbc.M104467200, ISSN: 0021-9258.

Schneider C, Tallman KA, Porter NA, Brash AR. Two distinct pathways of formation of 4-hydroxynonenal. Mechanisms of nonenzymatic transformation of the 9- and 13-hydroperoxides of linoleic acid to 4-hydroxyalkenals. J. Biol. Chem [print-electronic]. 2001 Jun 6/15/2001; 276(24): 20831-8. PMID: 11259420, PII: M101821200, DOI: 10.1074/jbc.M101821200, ISSN: 0021-9258.

Tijet N, Schneider C, Muller BL, Brash AR. Biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (CYP74C) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids. Arch. Biochem. Biophys. 2001 Feb 2/15/2001; 386(2): 281-9. PMID: 11368353, PII: S0003-9861(00)92218-5, DOI: 10.1006/abbi.2000.2218, ISSN: 0003-9861.

Schneider C, Keeney DS, Boeglin WE, Brash AR. Detection and cellular localization of 12R-lipoxygenase in human tonsils. Arch. Biochem. Biophys. 2001 Feb 2/15/2001; 386(2): 268-74. PMID: 11368351, PII: S0003-9861(00)92217-3, DOI: 10.1006/abbi.2000.2217, ISSN: 0003-9861.

Shappell SB, Gupta RA, Manning S, Whitehead R, Boeglin WE, Schneider C, Case T, Price J, Jack GS, Wheeler TM, Matusik RJ, Brash AR, Dubois RN. 15S-Hydroxyeicosatetraenoic acid activates peroxisome proliferator-activated receptor gamma and inhibits proliferation in PC3 prostate carcinoma cells. Cancer Res. 2001 Jan 1/15/2001; 61(2): 497-503. PMID: 11212240, ISSN: 0008-5472.

Schneider C, Boeglin WE, Brash AR. Enantiomeric separation of hydroxy eicosanoids by chiral column chromatography: effect of the alcohol modifier. Anal. Biochem. 2000 Dec 12/1/2000; 287(1): 186-9. PMID: 11078601, PII: S0003-2697(00)94847-4, DOI: 10.1006/abio.2000.4847, ISSN: 0003-2697.

Schneider C, Boeglin WE, Lai S, Cha JK, Brash AR. Synthesis and applications of stereospecifically (3)H-labeled arachidonic acids as mechanistic probes for lipoxygenase and cyclooxygenase catalysis. Anal. Biochem. 2000 Aug 8/15/2000; 284(1): 125-35. PMID: 10933865, PII: S0003269700946700, DOI: 10.1006/abio.2000.4670, ISSN: 0003-2697.

Rowlinson SW, Crews BC, Goodwin DC, Schneider C, Gierse JK, Marnett LJ. Spatial requirements for 15-(R)-hydroxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid synthesis within the cyclooxygenase active site of murine COX-2. Why acetylated COX-1 does not synthesize 15-(R)-hete. J. Biol. Chem. 2000 Mar 3/3/2000; 275(9): 6586-91. PMID: 10692466, ISSN: 0021-9258.

Schneider C, Brash AR. Stereospecificity of hydrogen abstraction in the conversion of arachidonic acid to 15R-HETE by aspirin-treated cyclooxygenase-2. Implications for the alignment of substrate in the active site. J. Biol. Chem. 2000 Feb 2/18/2000; 275(7): 4743-6. PMID: 10671506, ISSN: 0021-9258.

Schneider, C. and Schreier, P. Catalytic properties of allene oxide synthase from flaxseed (Linum usitatissimum L.). Lipids. 1998; 33: 191-6.

Schneider, C., Amberg, A., Feurle, J., Ross, A., Roth, M., Toth, G., and Schreier, P. 2-[4'-Hydroxy-3'-methoxy)-phenoxy]-4-(4"-hydroxy-3"-methoxy-phenyl)-8-hydroxy-6-oxo-3-oxabicyclo[3.3.0]-7-octene: Unusual product of the soybean lipoxygenase-catalyzed oxygenation of curcumin. J. Mol. Catal. B: Enzymatic. 1998; 4: 219-27.

Herderich, M., Richling, E., Roscher, R., Schneider, C., Schwab, W., Humpf, H.-U., and Schreier, P. Application of atmospheric pressure ionization HPLC-MS-MS for the analysis of natural products. Chromatographia. 1997; 45: 127-32.

Schneider, C., Schreier, P., and Herderich, M. Analysis of lipoxygenase-derived fatty acid hydroperoxides by electrospray ionization tandem mass spectrometry. Lipids. 1997; 32: 331-6.

Schneider, C., Wein, M., Harmsen, D., and Schreier, P. A fatty acid alpha-ketol, a product of the plant lipoxygenase pathway, is oxidized to 3(Z)-dodecendioic acid by a bacterial monooxygenase. Biochem. Biophys. Res. Commun. 1997; 232: 364-6.

Schneider, C., Schreier, P., and Humpf, H.-U. Exciton-coupled circular dichroism (ECCD) in acyclic hydroxylated dienes: A sensitive method for the direct stereochemical assignment of lipoxygenase products. Chirality. 1997; 9: 563-7.

Available Postdoctoral Position Details
Posted: 2/19/2014
NIH-funded positions available for a start date of October 1, 2013. Candidates should have experience in structural analysis of small molecules using HPLC, NMR, LC-MS. A background in lipid or natural product biochemistry would be a big plus. We also welcome candidates that, in addition, are proficient in cell culture and molecular biology.
Our laboratory encourages international students to apply for post-graduate research. In particular, we are interested in recruiting Brazilian Science without Borders fellows. For information please contact the PI.