Tina M. Iverson, Ph.D.



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Faculty Appointments
Professor of Pharmacology Professor of BiochemistryLouise B. McGavock Chair
Ph.D., Biochemistry, California Institute of Technology, Pasadena, CaliforniaB.S., Chemistry, St. John's University, Jamaica, New York
Office Address
460 RRB; Department of Pharmacology
Vanderbilt University Medical Center
23rd Ave @ Pierce
Nashville, TN 37232-6600
Research Description
The fundamental ability of an organism to respond to information in the environment is critical to many broad areas of biology. For example, during organ and tissue development, how do cells determine when to grow or when to die? How does a host know that a pathogen is present, and how does a pathogen identify an appropriate host? Proteins commonly store information, and these proteins may respond to changes in information via changes in shape (conformational changes) or changes in oligomerization. To reveal how information is encoded and transferred, we use a molecules-to-organisms approach where we first determine structures of the proteins that regulate any given process. We then investigate how these structures change in the presence of a signal. We develop hypotheses for how the information is encoded, and test these in vitro, in cells, and sometimes in whole organisms. We find that there are distinct guiding principles for information encoding that may be common to many signaling systems.

Research Keywords
Biological information, structural biology, cryoEM, crystallography, signaling, chemotaxis, glycobiology, protein engineering, energy transduction, mitochondrial function
Sharna P, Maklashina E, Voehler M, Balintova S, Dvorakova S, Kraus M, Hadrava Vanova K, Nahacka Z, Zobalova R, Boukalova S, Cunatova K, Mracek T, Ghayee HK, Pacek K, Rohlena J, Neuzil J, Cecchini G, Iverson TM, et al. Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners. Nat communications. 2024 Jan 1/11/2024; 15: 473.

Dulin CC, Sharma P, Frigo L, Voehler MW, Iverson TM, Bachmann BO. EvdS6 is a bifunctional decarboxylase from the everninomicin gene cluster. J Biol Chem [print-electronic]. 2023 Jul; 299(7): 104893. PMID: 37286037, PMCID: PMC10338323, PII: S0021-9258(23)01921-X, DOI: 10.1016/j.jbc.2023.104893, ISSN: 1083-351X.

Iverson TM, Singh PK, Cecchini G. An evolving view of complex II-noncanonical complexes, megacomplexes, respiration, signaling, and beyond. J Biol Chem [print-electronic]. 2023 Jun; 299(6): 104761. PMID: 37119852, PMCID: PMC10238741, PII: S0021-9258(23)01789-1, DOI: 10.1016/j.jbc.2023.104761, ISSN: 1083-351X.

Tomar R, Minko IG, Sharma P, Kellum AH, Lei L, Harp JM, Iverson TM, Lloyd RS, Egli M, Stone MP. Base excision repair of the N-(2-deoxy-d-erythro-pentofuranosyl)-urea lesion by the hNEIL1 glycosylase. Nucleic Acids Res. 2023 May 5/8/2023; 51(8): 3754-69. PMID: 37014002, PMCID: PMC10164570, PII: 7103208, DOI: 10.1093/nar/gkad164, ISSN: 1362-4962.

Singh PK, Cecchini G, Nakagawa T, Iverson TM. CryoEM structure of a post-assembly MS-ring reveals plasticity in stoichiometry and conformation. PLoS One. 2023; 18(5): e0285343. PMID: 37205674, PMCID: PMC10198558, PII: PONE-D-23-07069, DOI: 10.1371/journal.pone.0285343, ISSN: 1932-6203.

Maklashina E, Iverson TM, Cecchini G. How an assembly factor enhances covalent FAD attachment to the flavoprotein subunit of complex II. J Biol Chem [print-electronic]. 2022 Oct; 298(10): 102472. PMID: 36089066, PMCID: PMC9557727, PII: S0021-9258(22)00915-2, DOI: 10.1016/j.jbc.2022.102472, ISSN: 1083-351X.

Perry-Hauser NA, Kaoud TS, Stoy H, Zhan X, Chen Q, Dalby KN, Iverson TM, Gurevich VV, Gurevich EV. Short Arrestin-3-Derived Peptides Activate JNK3 in Cells. Int J Mol Sci. 2022 Aug 8/4/2022; 23(15): PMID: 35955810, PMCID: PMC9368909, PII: ijms23158679, DOI: 10.3390/ijms23158679, ISSN: 1422-0067.

Bensing BA, Stubbs HE, Agarwal R, Yamakawa I, Luong K, Solakyildirim K, Yu H, Hadadianpour A, Castro MA, Fialkowski KP, Morrison KM, Wawrzak Z, Chen X, Lebrilla CB, Baudry J, Smith JC, Sullam PM, Iverson TM. Origins of glycan selectivity in streptococcal Siglec-like adhesins suggest mechanisms of receptor adaptation. Nat Commun. 2022 May 5/18/2022; 13(1): 2753. PMID: 35585145, PMCID: PMC9117288, PII: 10.1038/s41467-022-30509-y, DOI: 10.1038/s41467-022-30509-y, ISSN: 2041-1723.

Perry-Hauser NA, Hopkins JB, Zhuo Y, Zheng C, Perez I, Schultz KM, Vishnivetskiy SA, Kaya AI, Sharma P, Dalby KN, Chung KY, Klug CS, Gurevich VV, Iverson TM. The Two Non-Visual Arrestins Engage ERK2 Differently. J Mol Biol [print-electronic]. 2022 Apr 4/15/2022; 434(7): 167465. PMID: 35077767, PMCID: PMC8977243, PII: S0022-2836(22)00029-8, DOI: 10.1016/j.jmb.2022.167465, ISSN: 1089-8638.

Perez I, Berndt S, Agarwal R, Castro MA, Vishnivetskiy SA, Smith JC, Sanders CR, Gurevich VV, Iverson TM. A Model for the Signal Initiation Complex Between Arrestin-3 and the Src Family Kinase Fgr. J Mol Biol [print-electronic]. 2022 Jan 1/30/2022; 434(2): 167400. PMID: 34902430, PMCID: PMC8752512, PII: S0022-2836(21)00637-9, DOI: 10.1016/j.jmb.2021.167400, ISSN: 1089-8638.

Qu C, Park JY, Yun MW, He QT, Yang F, Kim K, Ham D, Li RR, Iverson TM, Gurevich VV, Sun JP, Chung KY. Scaffolding mechanism of arrestin-2 in the cRaf/MEK1/ERK signaling cascade. Proc Natl Acad Sci U S A. 2021 Sep 9/14/2021; 118(37): PMID: 34507982, PMCID: PMC8449410, PII: 2026491118, DOI: 10.1073/pnas.2026491118, ISSN: 1091-6490.

Brown BL, Iverson TM. Handling heme with care. Nat Chem Biol. 2021 Jul; 17(7): 751-2. PMID: 34103722, PII: 10.1038/s41589-021-00821-7, DOI: 10.1038/s41589-021-00821-7, ISSN: 1552-4469.

Nason R, Büll C, Konstantinidi A, Sun L, Ye Z, Halim A, Du W, Sørensen DM, Durbesson F, Furukawa S, Mandel U, Joshi HJ, Dworkin LA, Hansen L, David L, Iverson TM, Bensing BA, Sullam PM, Varki A, Vries E, de Haan CAM, Vincentelli R, Henrissat B, Vakhrushev SY, Clausen H, Narimatsu Y. Display of the human mucinome with defined O-glycans by gene engineered cells. Nat Commun. 2021 Jul 7/1/2021; 12(1): 4070. PMID: 34210959, PMCID: PMC8249670, PII: 10.1038/s41467-021-24366-4, DOI: 10.1038/s41467-021-24366-4, ISSN: 2041-1723.

Chen Q, Zhuo Y, Sharma P, Perez I, Francis DJ, Chakravarthy S, Vishnivetskiy SA, Berndt S, Hanson SM, Zhan X, Brooks EK, Altenbach C, Hubbell WL, Klug CS, Iverson TM, Gurevich VV. An Eight Amino Acid Segment Controls Oligomerization and Preferred Conformation of the two Non-visual Arrestins. J Mol Biol [print-electronic]. 2021 Feb 2/19/2021; 433(4): 166790. PMID: 33387531, PMCID: PMC7870585, PII: S0022-2836(20)30715-4, DOI: 10.1016/j.jmb.2020.166790, ISSN: 1089-8638.

Limbrick EM, Yñigez-Gutierrez AE, Dulin CC, Derewacz DK, Spraggins JM, McCulloch KM, Iverson TM, Bachmann BO. Methyltransferase Contingencies in the Pathway of Everninomicin D Antibiotics and Analogues. Chembiochem [print-electronic]. 2020 Dec 12/1/2020; 21(23): 3349-58. PMID: 32686210, DOI: 10.1002/cbic.202000305, ISSN: 1439-7633.

Limbrick EM, Graf M, Derewacz DK, Nguyen F, Spraggins JM, Wieland M, Ynigez-Gutierrez AE, Reisman BJ, Zinshteyn B, McCulloch KM, Iverson TM, Green R, Wilson DN, Bachmann BO. Bifunctional Nitrone-Conjugated Secondary Metabolite Targeting the Ribosome. J Am Chem Soc [print-electronic]. 2020 Oct 10/28/2020; 142(43): 18369-77. PMID: 32709196, PMCID: PMC8129991, DOI: 10.1021/jacs.0c04675, ISSN: 1520-5126.

Stubbs HE, Bensing BA, Yamakawa I, Sharma P, Yu H, Chen X, Sullam PM, Iverson TM. Tandem sialoglycan-binding modules in a Streptococcus sanguinis serine-rich repeat adhesin create target dependent avidity effects. J Biol Chem [print-electronic]. 2020 Oct 10/23/2020; 295(43): 14737-49. PMID: 32820052, PMCID: PMC7586212, PII: S0021-9258(17)49350-1, DOI: 10.1074/jbc.RA120.014177, ISSN: 1083-351X.

Agarwal R, Bensing BA, Mi D, Vinson PN, Baudry J, Iverson TM, Smith JC. Structure based virtual screening identifies small molecule effectors for the sialoglycan binding protein Hsa. Biochem J. 2020 Oct 10/16/2020; 477(19): 3695-707. PMID: 32910185, PII: 226379, DOI: 10.1042/BCJ20200332, ISSN: 1470-8728.

Sharma P, Maklashina E, Cecchini G, Iverson TM. The roles of SDHAF2 and dicarboxylate in covalent flavinylation of SDHA, the human complex II flavoprotein. Proc Natl Acad Sci U S A [print-electronic]. 2020 Sep 9/22/2020; 117(38): 23548-56. PMID: 32887801, PMCID: PMC7519310, PII: 2007391117, DOI: 10.1073/pnas.2007391117, ISSN: 1091-6490.

Kaya AI, Perry NA, Gurevich VV, Iverson TM. Phosphorylation barcode-dependent signal bias of the dopamine D1 receptor. Proc Natl Acad Sci U S A [print-electronic]. 2020 Jun 6/23/2020; 117(25): 14139-49. PMID: 32503917, PMCID: PMC7321966, PII: 1918736117, DOI: 10.1073/pnas.1918736117, ISSN: 1091-6490.

Sharma P, Maklashina E, Cecchini G, Iverson TM. Maturation of the respiratory complex II flavoprotein. Curr. Opin. Struct. Biol [print-electronic]. 2019 Dec; 59: 38-46. PMID: 30851631, PMCID: PMC6731172, PII: S0959-440X(18)30142-8, DOI: 10.1016/j.sbi.2019.01.027, ISSN: 1879-033X.

Perry NA, Fialkowski KP, Kaoud TS, Kaya AI, Chen AL, Taliaferro JM, Gurevich VV, Dalby KN, Iverson TM. Arrestin-3 interaction with maternal embryonic leucine-zipper kinase. Cell. Signal [print-electronic]. 2019 Nov; 63: 109366. PMID: 31352007, PMCID: PMC6717526, PII: S0898-6568(19)30162-7, DOI: 10.1016/j.cellsig.2019.109366, ISSN: 1873-3913.

Sammons RM, Perry NA, Li Y, Cho EJ, Piserchio A, Zamora-Olivares DP, Ghose R, Kaoud TS, Debevec G, Bartholomeusz C, Gurevich VV, Iverson TM, Giulianotti M, Houghten RA, Dalby KN. A Novel Class of Common Docking Domain Inhibitors That Prevent ERK2 Activation and Substrate Phosphorylation. ACS Chem Biol [print-electronic]. 2019 Jun 6/21/2019; 14(6): 1183-94. PMID: 31058487, PMCID: PMC7105935, DOI: 10.1021/acschembio.9b00093, ISSN: 1554-8937.

Bensing BA, Li L, Yakovenko O, Wong M, Barnard KN, Iverson TM, Lebrilla CB, Parrish CR, Thomas WE, Xiong Y, Sullam PM. Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection. PLoS Pathog. 2019 Jun; 15(6): e1007896. PMID: 31233555, PMCID: PMC6611644, PII: PPATHOGENS-D-19-00300, DOI: 10.1371/journal.ppat.1007896, ISSN: 1553-7374.

McCulloch KM, Yamakawa I, Shifrin DA, McConnell RE, Foegeding NJ, Singh PK, Mao S, Tyska MJ, Iverson TM. An alternative N-terminal fold of the intestine-specific annexin A13a induces dimerization and regulates membrane-binding. J. Biol. Chem [print-electronic]. 2019 Mar 3/8/2019; 294(10): 3454-63. PMID: 30610115, PMCID: PMC6416438, PII: RA118.004571, DOI: 10.1074/jbc.RA118.004571, ISSN: 1083-351X.

Perry NA, Kaoud TS, Ortega OO, Kaya AI, Marcus DJ, Pleinis JM, Berndt S, Chen Q, Zhan X, Dalby KN, Lopez CF, Iverson TM, Gurevich VV. Arrestin-3 scaffolding of the JNK3 cascade suggests a mechanism for signal amplification. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2019 Jan 1/15/2019; 116(3): 810-5. PMID: 30591558, PMCID: PMC6338856, PII: 1819230116, DOI: 10.1073/pnas.1819230116, ISSN: 1091-6490.

Berndt S, Gurevich VV, Iverson TM. Crystal structure of the SH3 domain of human Lyn non-receptor tyrosine kinase. PLoS ONE. 2019; 14(4): e0215140. PMID: 30969999, PMCID: PMC6457566, PII: PONE-D-19-02846, DOI: 10.1371/journal.pone.0215140, ISSN: 1932-6203.

Perry NA, Zhan X, Gurevich EV, Iverson TM, Gurevich VV. Using In Vitro Pull-Down and In-Cell Overexpression Assays to Study Protein Interactions with Arrestin. Methods Mol. Biol. 2019; 1957: 107-20. PMID: 30919350, PMCID: PMC7039183, DOI: 10.1007/978-1-4939-9158-7_7, ISSN: 1940-6029.

Starbird CA, Perry NA, Chen Q, Berndt S, Yamakawa I, Loukachevitch LV, Limbrick EM, Bachmann BO, Iverson TM, McCulloch KM. The Structure of the Bifunctional Everninomicin Biosynthetic Enzyme EvdMO1 Suggests Independent Activity of the Fused Methyltransferase-Oxidase Domains. Biochemistry [print-electronic]. 2018 Dec 12/18/2018; 57(50): 6827-37. PMID: 30525509, PMCID: PMC7231510, DOI: 10.1021/acs.biochem.8b00836, ISSN: 1520-4995.

Chen Q, Iverson TM, Gurevich VV. Structural Basis of Arrestin-Dependent Signal Transduction. Trends Biochem. Sci [print-electronic]. 2018 Jun; 43(6): 412-23. PMID: 29636212, PMCID: PMC5959776, PII: S0968-0004(18)30058-6, DOI: 10.1016/j.tibs.2018.03.005, ISSN: 0968-0004.

Maklashina E, Rajagukguk S, Iverson TM, Cecchini G. The unassembled flavoprotein subunits of human and bacterial complex II have impaired catalytic activity and generate only minor amounts of ROS. J. Biol. Chem [print-electronic]. 2018 May 5/18/2018; 293(20): 7754-65. PMID: 29610278, PMCID: PMC5961047, PII: RA118.001977, DOI: 10.1074/jbc.RA118.001977, ISSN: 1083-351X.

Starbird CA, Tomasiak TM, Singh PK, Yankovskaya V, Maklashina E, Eisenbach M, Cecchini G, Iverson TM. New crystal forms of the integral membrane Escherichia coli quinol:fumarate reductase suggest that ligands control domain movement. J. Struct. Biol [print-electronic]. 2018 Apr; 202(1): 100-4. PMID: 29158068, PMCID: PMC5835405, PII: S1047-8477(17)30193-4, DOI: 10.1016/j.jsb.2017.11.004, ISSN: 1095-8657.

Sharma P, Maklashina E, Cecchini G, Iverson TM. Crystal structure of an assembly intermediate of respiratory Complex II. Nat Commun. 2018 Jan 1/18/2018; 9(1): 274. PMID: 29348404, PMCID: PMC5773532, PII: 10.1038/s41467-017-02713-8, DOI: 10.1038/s41467-017-02713-8, ISSN: 2041-1723.

Tso SC, Chen Q, Vishnivetskiy SA, Gurevich VV, Iverson TM, Brautigam CA. Using two-site binding models to analyze microscale thermophoresis data. Anal. Biochem [print-electronic]. 2018 Jan 1/1/2018; 540-541: 64-75. PMID: 29054528, PMCID: PMC5906060, PII: S0003-2697(17)30398-6, DOI: 10.1016/j.ab.2017.10.013, ISSN: 1096-0309.

Chen Q, Perry NA, Vishnivetskiy SA, Berndt S, Gilbert NC, Zhuo Y, Singh PK, Tholen J, Ohi MD, Gurevich EV, Brautigam CA, Klug CS, Gurevich VV, Iverson TM. Structural basis of arrestin-3 activation and signaling. Nat Commun. 2017 Nov 11/10/2017; 8(1): 1427. PMID: 29127291, PMCID: PMC5681653, PII: 10.1038/s41467-017-01218-8, DOI: 10.1038/s41467-017-01218-8, ISSN: 2041-1723.

Starbird CA, Maklashina E, Sharma P, Qualls-Histed S, Cecchini G, Iverson TM. Structural and biochemical analyses reveal insights into covalent flavinylation of the Escherichia coli Complex II homolog quinol:fumarate reductase. J. Biol. Chem [print-electronic]. 2017 Aug 8/4/2017; 292(31): 12921-33. PMID: 28615448, PMCID: PMC5546032, PII: M117.795120, DOI: 10.1074/jbc.M117.795120, ISSN: 1083-351X.

Starbird CA, Maklashina E, Sharma P, Qualls-Histed S, Cecchini G, Iverson TM. Structural and biochemical analyses reveal insights into covalent flavinylation of the Escherichia coli Complex II homolog quinol:fumarate reductase. J. Biol. Chem [print-electronic]. 2017 Aug 8/4/2017; 292(31): 12921-33. PMID: 28615448, PMCID: PMC5546032, PII: M117.795120, DOI: 10.1074/jbc.M117.795120, ISSN: 1083-351X.

Prokop S, Perry NA, Vishnivetskiy SA, Toth AD, Inoue A, Milligan G, Iverson TM, Hunyady L, Gurevich VV. Differential manipulation of arrestin-3 binding to basal and agonist-activated G protein-coupled receptors. Cell. Signal [print-electronic]. 2017 Aug; 36: 98-107. PMID: 28461104, PII: S0898-6568(17)30121-3, DOI: 10.1016/j.cellsig.2017.04.021, ISSN: 1873-3913.

Prokop S, Perry NA, Vishnivetskiy SA, Toth AD, Inoue A, Milligan G, Iverson TM, Hunyady L, Gurevich VV. Differential manipulation of arrestin-3 binding to basal and agonist-activated G protein-coupled receptors. Cell. Signal [print-electronic]. 2017 Aug; 36: 98-107. PMID: 28461104, PMCID: PMC5797668, PII: S0898-6568(17)30121-3, DOI: 10.1016/j.cellsig.2017.04.021, ISSN: 1873-3913.

Loukachevitch LV, Bensing BA, Yu H, Zeng J, Chen X, Sullam PM, Iverson TM. Structures of the Streptococcus sanguinis SrpA Binding Region with Human Sialoglycans Suggest Features of the Physiological Ligand. Biochemistry [print-electronic]. 2016 Oct 10/11/2016; PMID: 27685666, DOI: 10.1021/acs.biochem.6b00704, ISSN: 1520-4995.

Kaya AI, Lokits AD, Gilbert JA, Iverson TM, Meiler J, Hamm HE. A conserved hydrophobic core in Gai1 regulates G protein activation and release from activated receptor. J. Biol. Chem [print-electronic]. 2016 Jul 7/26/2016; PMID: 27462082, PII: M116.745513, DOI: 10.1074/jbc.M116.745513, ISSN: 1083-351X.

Bensing BA, Loukachevitch LV, McCulloch KM, Yu H, Vann KR, Wawrzak Z, Anderson S, Chen X, Sullam PM, Iverson TM. Structural Basis for Sialoglycan Binding by the Streptococcus sanguinis SrpA Adhesin. J. Biol. Chem [print-electronic]. 2016 Apr 4/1/2016; 291(14): 7230-40. PMID: 26833566, PII: M115.701425, DOI: 10.1074/jbc.M115.701425, ISSN: 1083-351X.

Bensing BA, Khedri Z, Deng L, Yu H, Prakobphol A, Fisher SJ, Chen X, Iverson TM, Varki A, Sullam PM. Novel aspects of sialoglycan recognition by the Siglec-like domains of streptococcal SRR glycoproteins. Glycobiology [print-electronic]. 2016 Apr 4/1/2016; PMID: 27037304, PII: cww042, DOI: 10.1093/glycob/cww042, ISSN: 1460-2423.

Maklashina E, Rajagukguk S, Starbird CA, McDonald WH, Koganitsky A, Eisenbach M, Iverson TM, Cecchini G. Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II. J. Biol. Chem [print-electronic]. 2016 Feb 2/5/2016; 291(6): 2904-16. PMID: 26644464, PMCID: PMC4742753, PII: M115.690396, DOI: 10.1074/jbc.M115.690396, ISSN: 1083-351X.

Zhan X, Stoy H, Kaoud TS, Perry NA, Chen Q, Perez A, Els-Heindl S, Slagis JV, Iverson TM, Beck-Sickinger AG, Gurevich EV, Dalby KN, Gurevich VV. Peptide mini-scaffold facilitates JNK3 activation in cells. Sci Rep. 2016; 6: 21025. PMID: 26868142, PMCID: PMC4751492, PII: srep21025, DOI: 10.1038/srep21025, ISSN: 2045-2322.

McCulloch KM, McCranie EK, Smith JA, Sarwar M, Mathieu JL, Gitschlag BL, Du Y, Bachmann BO, Iverson TM. Oxidative cyclizations in orthosomycin biosynthesis expand the known chemistry of an oxygenase superfamily. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Sep 9/15/2015; 112(37): 11547-52. PMID: 26240321, PMCID: PMC4577193, PII: 1500964112, DOI: 10.1073/pnas.1500964112, ISSN: 1091-6490.

Chen Q, Vishnivetskiy SA, Zhuang T, Cho MK, Thaker TM, Sanders CR, Gurevich VV, Iverson TM. The rhodopsin-arrestin-1 interaction in bicelles. Methods Mol. Biol. 2015; 1271: 77-95. PMID: 25697518, PMCID: PMC4520306, DOI: 10.1007/978-1-4939-2330-4_6, ISSN: 1940-6029.

Kaya AI, Iverson TM, Hamm HE. Functional stability of rhodopsin in a bicelle system: evaluating G protein activation by rhodopsin in bicelles. Methods Mol. Biol. 2015; 1271: 67-76. PMID: 25697517, DOI: 10.1007/978-1-4939-2330-4_5, ISSN: 1940-6029.

Kaya AI, Lokits AD, Gilbert JA, Iverson TM, Meiler J, Hamm HE. A conserved phenylalanine as a relay between the a5 helix and the GDP binding region of heterotrimeric Gi protein a subunit. J. Biol. Chem [print-electronic]. 2014 Aug 8/29/2014; 289(35): 24475-87. PMID: 25037222, PMCID: PMC4148873, PII: M114.572875, DOI: 10.1074/jbc.M114.572875, ISSN: 1083-351X.

Birmingham WR, Starbird CA, Panosian TD, Nannemann DP, Iverson TM, Bachmann BO. Bioretrosynthetic construction of a didanosine biosynthetic pathway. Nat. Chem. Biol [print-electronic]. 2014 May; 10(5): 392-9. PMID: 24657930, PMCID: PMC4017637, PII: nchembio.1494, DOI: 10.1038/nchembio.1494, ISSN: 1552-4469.

Thaker TM, Sarwar M, Preininger AM, Hamm HE, Iverson TM. A transient interaction between the phosphate binding loop and switch I contributes to the allosteric network between receptor and nucleotide in Gai1. J. Biol. Chem [print-electronic]. 2014 Apr 4/18/2014; 289(16): 11331-41. PMID: 24596087, PMCID: PMC4036270, PII: M113.539064, DOI: 10.1074/jbc.M113.539064, ISSN: 1083-351X.

Vishnivetskiy SA, Zhan X, Chen Q, Iverson TM, Gurevich VV. Arrestin expression in E. coli and purification. Curr Protoc Pharmacol. 2014; 67: Unit 2.11.. PMID: 25446290, PMCID: PMC4260927, DOI: 10.1002/0471141755.ph0211s67, ISSN: 1934-8290.

Seo HS, Minasov G, Seepersaud R, Doran KS, Dubrovska I, Shuvalova L, Anderson WF, Iverson TM, Sullam PM. Characterization of fibrinogen binding by glycoproteins Srr1 and Srr2 of Streptococcus agalactiae. J. Biol. Chem [print-electronic]. 2013 Dec 12/13/2013; 288(50): 35982-96. PMID: 24165132, PMCID: PMC3861647, PII: M113.513358, DOI: 10.1074/jbc.M113.513358, ISSN: 1083-351X.

Singh PK, Sarwar M, Maklashina E, Kotlyar V, Rajagukguk S, Tomasiak TM, Cecchini G, Iverson TM. Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase. J. Biol. Chem [print-electronic]. 2013 Aug 8/23/2013; 288(34): 24293-301. PMID: 23836905, PMCID: PMC3750132, PII: M113.487082, DOI: 10.1074/jbc.M113.487082, ISSN: 1083-351X.

Iverson TM. Catalytic mechanisms of complex II enzymes: a structural perspective. Biochim. Biophys. Acta [print-electronic]. 2013 May; 1827(5): 648-57. PMID: 22995215, PMCID: PMC3537904, PII: S0005-2728(12)01031-6, DOI: 10.1016/j.bbabio.2012.09.008, ISSN: 0006-3002.

Vishnivetskiy SA, Chen Q, Palazzo MC, Brooks EK, Altenbach C, Iverson TM, Hubbell WL, Gurevich VV. Engineering visual arrestin-1 with special functional characteristics. J. Biol. Chem [print-electronic]. 2013 Feb 2/1/2013; 288(5): 3394-405. PMID: 23250748, PMCID: PMC3561558, PII: M112.445437, DOI: 10.1074/jbc.M112.445437, ISSN: 1083-351X.

Thaker TM, Tanabe M, Fowler ML, Preininger AM, Ingram-Smith C, Smith KS, Iverson TM. Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans. J. Struct. Biol [print-electronic]. 2013 Feb; 181(2): 185-9. PMID: 23159802, PMCID: PMC3565045, PII: S1047-8477(12)00313-9, DOI: 10.1016/j.jsb.2012.11.001, ISSN: 1095-8657.

Zhuang T, Chen Q, Cho MK, Vishnivetskiy SA, Iverson TM, Gurevich VV, Sanders CR. Involvement of distinct arrestin-1 elements in binding to different functional forms of rhodopsin. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Jan 1/15/2013; 110(3): 942-7. PMID: 23277586, PMCID: PMC3549108, PII: 1215176110, DOI: 10.1073/pnas.1215176110, ISSN: 1091-6490.

Iverson TM, Maklashina E, Cecchini G. Structural basis for malfunction in complex II. J. Biol. Chem [print-electronic]. 2012 Oct 10/12/2012; 287(42): 35430-8. PMID: 22904323, PMCID: PMC3471735, PII: R112.408419, DOI: 10.1074/jbc.R112.408419, ISSN: 1083-351X.

Iverson TM, Panosian TD, Birmingham WR, Nannemann DP, Bachmann BO. Molecular differences between a mutase and a phosphatase: investigations of the activation step in Bacillus cereus phosphopentomutase. Biochemistry [print-electronic]. 2012 Mar 3/6/2012; 51(9): 1964-75. PMID: 22329805, PMCID: PMC3302354, DOI: 10.1021/bi201761h, ISSN: 1520-4995.

Thaker TM, Kaya AI, Preininger AM, Hamm HE, Iverson TM. Allosteric mechanisms of G protein-Coupled Receptor signaling: a structural perspective. Methods Mol. Biol. 2012; 796: 133-74. PMID: 22052489, PMCID: PMC3549666, DOI: 10.1007/978-1-61779-334-9_8, ISSN: 1940-6029.

Pyburn TM, Bensing BA, Xiong YQ, Melancon BJ, Tomasiak TM, Ward NJ, Yankovskaya V, Oliver KM, Cecchini G, Sulikowski GA, Tyska MJ, Sullam PM, Iverson TM. A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors. PLoS Pathog [print-electronic]. 2011 Jul; 7(7): e1002112. PMID: 21765814, PMCID: PMC3131266, PII: PPATHOGENS-D-11-00123, DOI: 10.1371/journal.ppat.1002112, ISSN: 1553-7374.

Kaya AI, Thaker TM, Preininger AM, Iverson TM, Hamm HE. Coupling efficiency of rhodopsin and transducin in bicelles. Biochemistry [print-electronic]. 2011 Apr 4/19/2011; 50(15): 3193-203. PMID: 21375271, PMCID: PMC3119548, DOI: 10.1021/bi200037j, ISSN: 1520-4995.

Panosian TD, Nannemann DP, Watkins GR, Phelan VV, McDonald WH, Wadzinski BE, Bachmann BO, Iverson TM. Bacillus cereus phosphopentomutase is an alkaline phosphatase family member that exhibits an altered entry point into the catalytic cycle. J. Biol. Chem [print-electronic]. 2011 Mar 3/11/2011; 286(10): 8043-54. PMID: 21193409, PMCID: PMC3048691, PII: M110.201350, DOI: 10.1074/jbc.M110.201350, ISSN: 1083-351X.

Kuchtey J, Olson LM, Rinkoski T, Mackay EO, Iverson TM, Gelatt KN, Haines JL, Kuchtey RW. Mapping of the disease locus and identification of ADAMTS10 as a candidate gene in a canine model of primary open angle glaucoma. PLoS Genet [print-electronic]. 2011 Feb; 7(2): e1001306. PMID: 21379321, PMCID: PMC3040645, DOI: 10.1371/journal.pgen.1001306, ISSN: 1553-7404.

Tomasiak TM, Archuleta TL, Andréll J, Luna-Chávez C, Davis TA, Sarwar M, Ham AJ, McDonald WH, Yankovskaya V, Stern HA, Johnston JN, Maklashina E, Cecchini G, Iverson TM. Geometric restraint drives on- and off-pathway catalysis by the Escherichia coli menaquinol:fumarate reductase. J. Biol. Chem [print-electronic]. 2011 Jan 1/28/2011; 286(4): 3047-56. PMID: 21098488, PMCID: PMC3024798, PII: M110.192849, DOI: 10.1074/jbc.M110.192849, ISSN: 1083-351X.

Vey JL, Al-Mestarihi A, Hu Y, Funk MA, Bachmann BO, Iverson TM. Structure and mechanism of ORF36, an amino sugar oxidizing enzyme in everninomicin biosynthesis. Biochemistry. 2010 Nov 11/2/2010; 49(43): 9306-17. PMID: 20866105, PMCID: PMC2964426, DOI: 10.1021/bi101336u, ISSN: 1520-4995.

Pyburn TM, Yankovskaya V, Bensing BA, Cecchini G, Sullam PM, Iverson TM. Purification, crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding region of the Streptococcus gordonii adhesin GspB. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun [print-electronic]. 2010 Nov 11/1/2010; 66(Pt 11): 1503-7. PMID: 21045307, PMCID: PMC3001660, PII: S1744309110036535, DOI: 10.1107/S1744309110036535, ISSN: 1744-3091.

Panosian TD, Nannemann DP, Bachmann BO, Iverson TM. Crystallization and preliminary X-ray analysis of a phosphopentomutase from Bacillus cereus. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun [print-electronic]. 2010 Jul 7/1/2010; 66(Pt 7): 811-4. PMID: 20606280, PMCID: PMC2898468, PII: S1744309110017549, DOI: 10.1107/S1744309110017549, ISSN: 1744-3091.

Tanabe M, Nimigean CM, Iverson TM. Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2010 Apr 4/13/2010; 107(15): 6811-6. PMID: 20351243, PMCID: PMC2872391, PII: 0912115107, DOI: 10.1073/pnas.0912115107, ISSN: 1091-6490.

Thompson AN, Kim I, Panosian TD, Iverson TM, Allen TW, Nimigean CM. Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore. Nat. Struct. Mol. Biol [print-electronic]. 2009 Dec; 16(12): 1317-24. PMID: 19946269, PMCID: PMC2825899, PII: nsmb.1703, DOI: 10.1038/nsmb.1703, ISSN: 1545-9985.

Tanabe M, Iverson TM. Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun [print-electronic]. 2009 Oct 10/1/2009; 65(Pt 10): 996-1000. PMID: 19851005, PMCID: PMC2765884, PII: S1744309109032333, DOI: 10.1107/S1744309109032333, ISSN: 1744-3091.

Preininger AM, Funk MA, Oldham WM, Meier SM, Johnston CA, Adhikary S, Kimple AJ, Siderovski DP, Hamm HE, Iverson TM. Helix dipole movement and conformational variability contribute to allosteric GDP release in Galphai subunits. Biochemistry. 2009 Mar 3/31/2009; 48(12): 2630-42. PMID: 19222191, PMCID: PMC2736342, PII: 10.1021/bi801853a, DOI: 10.1021/bi801853a, ISSN: 1520-4995.

Adler DH, Phillips JA, Cogan JD, Iverson TM, Schnetz-Boutaud N, Stein JA, Brenner DA, Milne GL, Morrow JD, Boutaud O, Oates JA. The enteropathy of prostaglandin deficiency. J. Gastroenterol [print-electronic]. 2009; 44 Suppl 19: 1-7. PMID: 19148786, PMCID: PMC2799331, DOI: 10.1007/s00535-008-2253-y, ISSN: 0944-1174.

Adler DH, Cogan JD, Phillips JA, Schnetz-Boutaud N, Milne GL, Iverson T, Stein JA, Brenner DA, Morrow JD, Boutaud O, Oates JA. Inherited human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunction. J. Clin. Invest. 2008 Jun; 118(6): 2121-31. PMID: 18451993, PMCID: PMC2350426, DOI: 10.1172/JCI30473, ISSN: 0021-9738.

Tomasiak TM, Maklashina E, Cecchini G, Iverson TM. A threonine on the active site loop controls transition state formation in Escherichia coli respiratory complex II. J. Biol. Chem [print-electronic]. 2008 May 5/30/2008; 283(22): 15460-8. PMID: 18385138, PMCID: PMC2397489, PII: M801372200, DOI: 10.1074/jbc.M801372200, ISSN: 0021-9258.

Tomasiak TM, Cecchini G, Iverson TM. Succinate as Donor; Fumarate as Acceptor. EcoSal Plus. 2007 Apr; 2(2): PMID: 26443593, DOI: 10.1128/ecosal.3.2.6, ISSN: 2324-6200.

Maklashina E, Iverson TM, Sher Y, Kotlyar V, Andréll J, Mirza O, Hudson JM, Armstrong FA, Rothery RA, Weiner JH, Cecchini G. Fumarate reductase and succinate oxidase activity of Escherichia coli complex II homologs are perturbed differently by mutation of the flavin binding domain. J. Biol. Chem [print-electronic]. 2006 Apr 4/21/2006; 281(16): 11357-65. PMID: 16484232, PII: M512544200, DOI: 10.1074/jbc.M512544200, ISSN: 0021-9258.

Iverson TM. Evolution and unique bioenergetic mechanisms in oxygenic photosynthesis. Curr Opin Chem Biol [print-electronic]. 2006 Apr; 10(2): 91-100. PMID: 16504567, PII: S1367-5931(06)00031-7, DOI: 10.1016/j.cbpa.2006.02.013, ISSN: 1367-5931.

Ferreira KN, Iverson TM, Maghlaoui K, Barber J, Iwata S. Architecture of the photosynthetic oxygen-evolving center. Science [print-electronic]. 2004 Mar 3/19/2004; 303(5665): 1831-8. PMID: 14764885, PII: 1093087, DOI: 10.1126/science.1093087, ISSN: 1095-9203.

Cecchini G, Maklashina E, Yankovskaya V, Iverson TM, Iwata S. Variation in proton donor/acceptor pathways in succinate:quinone oxidoreductases. FEBS Lett. 2003 Jun 6/12/2003; 545(1): 31-8. PMID: 12788489, PII: S0014579303003909, ISSN: 0014-5793.

Doukov TI, Iverson TM, Seravalli J, Ragsdale SW, Drennan CL. A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase. Science. 2002 Oct 10/18/2002; 298(5593): 567-72. PMID: 12386327, PII: 298/5593/567, DOI: 10.1126/science.1075843, ISSN: 1095-9203.

Iyer R, Iverson TM, Accardi A, Miller C. A biological role for prokaryotic ClC chloride channels. Nature. 2002 Oct 10/17/2002; 419(6908): 715-8. PMID: 12384697, PII: nature01000, DOI: 10.1038/nature01000, ISSN: 0028-0836.

Iverson TM, Luna-Chavez C, Croal LR, Cecchini G, Rees DC. Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site. J. Biol. Chem [print-electronic]. 2002 May 5/3/2002; 277(18): 16124-30. PMID: 11850430, PII: M200815200, DOI: 10.1074/jbc.M200815200, ISSN: 0021-9258.

Iverson TM, Arciero DM, Hooper AB, Rees DC. High-resolution structures of the oxidized and reduced states of cytochrome c554 from Nitrosomonas europaea. J. Biol. Inorg. Chem. 2001 Apr; 6(4): 390-7. PMID: 11372197, ISSN: 0949-8257.

Strop P, Smith KS, Iverson TM, Ferry JG, Rees DC. Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum. J. Biol. Chem [print-electronic]. 2001 Mar 3/30/2001; 276(13): 10299-305. PMID: 11096105, PII: M009182200, DOI: 10.1074/jbc.M009182200, ISSN: 0021-9258.

Iverson TM, Alber BE, Kisker C, Ferry JG, Rees DC. A closer look at the active site of gamma-class carbonic anhydrases: high-resolution crystallographic studies of the carbonic anhydrase from Methanosarcina thermophila. Biochemistry. 2000 Aug 8/8/2000; 39(31): 9222-31. PMID: 10924115, PII: bi000204s, ISSN: 0006-2960.

Iverson TM, Luna-Chavez C, Schröder I, Cecchini G, Rees DC. Analyzing your complexes: structure of the quinol-fumarate reductase respiratory complex. Curr. Opin. Struct. Biol. 2000 Aug; 10(4): 448-55. PMID: 10981634, PII: S0959-440X(00)00113-5, ISSN: 0959-440X.

Luna-Chavez C, Iverson TM, Rees DC, Cecchini G. Overexpression, purification, and crystallization of the membrane-bound fumarate reductase from Escherichia coli. Protein Expr. Purif. 2000 Jun; 19(1): 188-96. PMID: 10833406, PII: S1046-5928(00)91238-5, DOI: 10.1006/prep.2000.1238, ISSN: 1046-5928.

Iverson TM, Luna-Chavez C, Cecchini G, Rees DC. Structure of the Escherichia coli fumarate reductase respiratory complex. Science. 1999 Jun 6/18/1999; 284(5422): 1961-6. PMID: 10373108, PII: 7600, ISSN: 0036-8075.

Iverson TM, Arciero DM, Hsu BT, Logan MS, Hooper AB, Rees DC. Heme packing motifs revealed by the crystal structure of the tetra-heme cytochrome c554 from Nitrosomonas europaea. Nat. Struct. Biol. 1998 Nov; 5(11): 1005-12. PMID: 9808046, DOI: 10.1038/2975, ISSN: 1072-8368.