Joyce Cheung-Flynn, Ph.D.

Research Associate Professor
Faculty Appointments
Research Associate Professor of Vascular Surgery
Ph.D., Molecular and Cellular Biology, Arizona State University, Tempe, ArizonaM.S., Biology, Northern Arizona University , Flagstaff, ArizonaB.S., Northern Arizona University , Flagstaff, Arizona
Office Address
D-5237 MCN
Nashville, TN 2735
Research Keywords
Vascular biology; peptide therapeutics; purinergic receptor; vein graft preservation; intimal hyperplasia; subarachnoid hemorrhage; sepsis; animal vascular disease models; translational medicine
Morone PJ, Yan W, Adcock J, Komalavilas P, Mocco J, Thompson RC, Brophy C, Cheung-Flynn J. Vasorelaxing cell permeant phosphopeptide mimetics for subarachnoid hemorrhage. Eur J Pharmacol [print-electronic]. 2021 Jun 6/5/2021; 900: 174038. PMID: 33737008, PII: S0014-2999(21)00191-6, DOI: 10.1016/j.ejphar.2021.174038, ISSN: 1879-0712.

Yim TW, Perling D, Polcz M, Komalavilas P, Brophy C, Cheung-Flynn J. A cell permeant phosphopeptide mimetic of Niban inhibits p38 MAPK and restores endothelial function after injury. FASEB J [print-electronic]. 2020 Jul; 34(7): 9180-91. PMID: 32396246, PMCID: PMC7383822, DOI: 10.1096/fj.201902745R, ISSN: 1530-6860.

Meegan JE, Shaver CM, Putz ND, Jesse JJ, Landstreet SR, Lee HNR, Sidorova TN, McNeil JB, Wynn JL, Cheung-Flynn J, Komalavilas P, Brophy CM, Ware LB, Bastarache JA. Cell-free hemoglobin increases inflammation, lung apoptosis, and microvascular permeability in murine polymicrobial sepsis. PLoS One. 2020; 15(2): e0228727. PMID: 32012200, PMCID: PMC6996826, PII: PONE-D-19-12903, DOI: 10.1371/journal.pone.0228727, ISSN: 1932-6203.

Evans BC, Fletcher RB, Kilchrist KV, Dailing EA, Mukalel AJ, Colazo JM, Oliver M, Cheung-Flynn J, Brophy CM, Tierney JW, Isenberg JS, Hankenson KD, Ghimire K, Lander C, Gersbach CA, Duvall CL. An anionic, endosome-escaping polymer to potentiate intracellular delivery of cationic peptides, biomacromolecules, and nanoparticles. Nat Commun. 2019 Nov 11/1/2019; 10(1): 5012. PMID: 31676764, PMCID: PMC6825215, PII: 10.1038/s41467-019-12906-y, DOI: 10.1038/s41467-019-12906-y, ISSN: 2041-1723.

Hocking KM, Evans BC, Komalavilas P, Cheung-Flynn J, Duvall CL, Brophy CM. Nanotechnology Enabled Modulation of Signaling Pathways Affects Physiologic Responses in Intact Vascular Tissue. Tissue Eng Part A [print-electronic]. 2019 Mar; 25(5-6): 416-26. PMID: 30132374, PMCID: PMC6450456, DOI: 10.1089/ten.TEA.2018.0169, ISSN: 1937-335X.

Cheung-Flynn J, Alvis BD, Hocking KM, Guth CM, Luo W, McCallister R, Chadalavada K, Polcz M, Komalavilas P, Brophy CM. Normal Saline solutions cause endothelial dysfunction through loss of membrane integrity, ATP release, and inflammatory responses mediated by P2X7R/p38 MAPK/MK2 signaling pathways. PLoS One. 2019; 14(8): e0220893. PMID: 31412063, PMCID: PMC6693757, PII: PONE-D-19-09635, DOI: 10.1371/journal.pone.0220893, ISSN: 1932-6203.

Mukalel AJ, Evans BC, Kilchrist KV, Dailing EA, Burdette B, Cheung-Flynn J, Brophy CM, Duvall CL. Excipients for the lyoprotection of MAPKAP kinase 2 inhibitory peptide nano-polyplexes. J Control Release [print-electronic]. 2018 Jul 7/28/2018; 282: 110-9. PMID: 29709529, PMCID: PMC6157733, PII: S0168-3659(18)30246-3, DOI: 10.1016/j.jconrel.2018.04.045, ISSN: 1873-4995.

Luo W, Feldman D, McCallister R, Brophy C, Cheung-Flynn J. P2X7R antagonism after subfailure overstretch injury of blood vessels reverses vasomotor dysfunction and prevents apoptosis. Purinergic Signal [print-electronic]. 2017 Dec; 13(4): 579-90. PMID: 28905300, PMCID: PMC5714848, PII: 10.1007/s11302-017-9585-0, DOI: 10.1007/s11302-017-9585-0, ISSN: 1573-9546.

Lee Y, Le Thi P, Seon GM, Ryu SB, Brophy CM, Kim Y, Park JC, Park KD, Cheung-Flynn J, Sung HJ. Heparin-functionalized polymer graft surface eluting MK2 inhibitory peptide to improve hemocompatibility and anti-neointimal activity. J Control Release [print-electronic]. 2017 Nov 11/28/2017; 266: 321-30. PMID: 28987880, PMCID: PMC5723561, PII: S0168-3659(17)30889-1, DOI: 10.1016/j.jconrel.2017.10.002, ISSN: 1873-4995.

Cheung-Flynn J, Song J, Voskresensky I, Wise ES, Liu Y, Xiong Y, Eagle SS, Brophy CM, Flynn CR. Limiting Injury During Saphenous Vein Graft Preparation For Coronary Arterial Bypass Prevents Metabolic Decompensation. Sci Rep. 2017 Oct 10/27/2017; 7(1): 14179. PMID: 29079734, PMCID: PMC5660200, PII: 10.1038/s41598-017-13819-w, DOI: 10.1038/s41598-017-13819-w, ISSN: 2045-2322.

Wise ES, Hocking KM, Evans BC, Duvall CL, Cheung-Flynn J, Brophy CM. Unregulated saphenous vein graft distension decreases tissue viscoelasticity. Perfusion [print-electronic]. 2017 Sep; 32(6): 489-94. PMID: 28820033, PMCID: PMC5967241, DOI: 10.1177/0267659117697814, ISSN: 1477-111X.

Guth CM, Luo W, Jolayemi O, Chadalavada KS, Komalavilas P, Cheung-Flynn J, Brophy CM. Adenosine triphosphate as a molecular mediator of the vascular response to injury. J Surg Res [print-electronic]. 2017 Aug; 216: 80-6. PMID: 28807217, PMCID: PMC5962343, PII: S0022-4804(17)30152-X, DOI: 10.1016/j.jss.2017.03.025, ISSN: 1095-8673.

Komalavilas P, Luo W, Guth CM, Jolayemi O, Bartelson RI, Cheung-Flynn J, Brophy CM. Vascular surgical stretch injury leads to activation of P2X7 receptors and impaired endothelial function. PLoS One. 2017; 12(11): e0188069. PMID: 29136654, PMCID: PMC5685620, PII: PONE-D-17-11266, DOI: 10.1371/journal.pone.0188069, ISSN: 1932-6203.

Pedigo SL, Guth CM, Hocking KM, Banathy A, Li FD, Cheung-Flynn J, Brophy CM, Guzman RJ, Komalavilas P. Calcification of Human Saphenous Vein Associated with Endothelial Dysfunction: A Pilot Histopathophysiological and Demographical Study. Front Surg. 2017; 4: 6. PMID: 28232909, PMCID: PMC5298955, DOI: 10.3389/fsurg.2017.00006, ISSN: 2296-875X.

Boire TC, Balikov DA, Lee Y, Guth CM, Cheung-Flynn J, Sung HJ. Biomaterial-Based Approaches to Address Vein Graft and Hemodialysis Access Failures. Macromol Rapid Commun [print-electronic]. 2016 Dec; 37(23): 1860-80. PMID: 27673474, PMCID: PMC5156561, DOI: 10.1002/marc.201600412, ISSN: 1521-3927.

Wise ES, Hocking KM, Luo W, Feldman DL, Song J, Komalavilas P, Cheung-Flynn J, Brophy CM. Traditional graft preparation decreases physiologic responses, diminishes viscoelasticity, and reduces cellular viability of the conduit: A porcine saphenous vein model. Vasc Med [print-electronic]. 2016 Oct; 21(5): 413-21. PMID: 27216870, PMCID: PMC5115945, PII: 1358863X16649040, DOI: 10.1177/1358863X16649040, ISSN: 1477-0377.

Osgood MJ, Sexton K, Voskresensky I, Hocking K, Song J, Komalavilas P, Brophy C, Cheung-Flynn J. Use of Brilliant Blue FCF during vein graft preparation inhibits intimal hyperplasia. J Vasc Surg. 2016 Aug; 64(2): 471-8. PMID: 27763268, PMCID: PMC5480606, PII: S0741-5214(15)00278-5, DOI: 10.1016/j.jvs.2015.02.028, ISSN: 1097-6809.

Banathy A, Cheung-Flynn J, Goleniewska K, Boyd KL, Newcomb DC, Peebles RS, Komalavilas P. Heat Shock-Related Protein 20 Peptide Decreases Human Airway Constriction Downstream of ß2-Adrenergic Receptor. Am J Respir Cell Mol Biol. 2016 Aug; 55(2): 225-33. PMID: 26909644, PMCID: PMC4979360, DOI: 10.1165/rcmb.2015-0139OC, ISSN: 1535-4989.

Hocking KM, Luo W, Li FD, Komalavilas P, Brophy C, Cheung-Flynn J. Brilliant blue FCF is a nontoxic dye for saphenous vein graft marking that abrogates response to injury. J Vasc Surg [print-electronic]. 2016 Jul; 64(1): 210-8. PMID: 25704409, PMCID: PMC4544660, PII: S0741-5214(15)00061-0, DOI: 10.1016/j.jvs.2014.12.059, ISSN: 1097-6809.

Luo W, Guth CM, Jolayemi O, Duvall CL, Brophy CM, Cheung-Flynn J. Subfailure Overstretch Injury Leads to Reversible Functional Impairment and Purinergic P2X7 Receptor Activation in Intact Vascular Tissue. Front Bioeng Biotechnol. 2016; 4: 75. PMID: 27747211, PMCID: PMC5040722, DOI: 10.3389/fbioe.2016.00075, ISSN: 2296-4185.

Wise ES, Cheung-Flynn J, Brophy CM. Standard Surgical Skin Markers Should Be Avoided for Intraoperative Vein Graft Marking during Cardiac and Peripheral Bypass Operations. Front Surg. 2016; 3: 36. PMID: 27379243, PMCID: PMC4913101, DOI: 10.3389/fsurg.2016.00036, ISSN: 2296-875X.

Hocking KM, Putumbaka G, Wise ES, Cheung-Flynn J, Brophy CM, Komalavilas P. Papaverine Prevents Vasospasm by Regulation of Myosin Light Chain Phosphorylation and Actin Polymerization in Human Saphenous Vein. PLoS One. 2016; 11(5): e0154460. PMID: 27136356, PMCID: PMC4852981, PII: PONE-D-15-27329, DOI: 10.1371/journal.pone.0154460, ISSN: 1932-6203.

Wise ES, Hocking KM, Eagle S, Absi T, Komalavilas P, Cheung-Flynn J, Brophy CM. Preservation solution impacts physiologic function and cellular viability of human saphenous vein graft. Surgery [print-electronic]. 2015 Aug; 158(2): 537-46. PMID: 26003912, PMCID: PMC4492846, PII: S0039-6060(15)00243-3, DOI: 10.1016/j.surg.2015.03.036, ISSN: 1532-7361.

Flynn CR, Albaugh VL, Cai S, Cheung-Flynn J, Williams PE, Brucker RM, Bordenstein SR, Guo Y, Wasserman DH, Abumrad NN. Bile diversion to the distal small intestine has comparable metabolic benefits to bariatric surgery. Nat Commun. 2015 Jul 7/21/2015; 6: 7715. PMID: 26197299, PMCID: PMC4518285, PII: ncomms8715, DOI: 10.1038/ncomms8715, ISSN: 2041-1723.

Wise ES, Hocking KM, Feldman D, Komalavilas P, Cheung-Flynn J, Brophy CM. An Optimized Preparation Technique for Saphenous Vein Graft. Am Surg. 2015 Jul; 81(7): E274-6. PMID: 26140874, PMCID: PMC4635024, ISSN: 1555-9823.

Voskresensky IV, Wise ES, Hocking KM, Li FD, Osgood MJ, Komalavilas P, Brophy C, Cheung-Flynn J. Brilliant blue FCF as an alternative dye for saphenous vein graft marking: effect on conduit function. JAMA Surg. 2014 Nov; 149(11): 1176-81. PMID: 25251505, PMCID: PMC4237645, PII: 1906151, DOI: 10.1001/jamasurg.2014.2029, ISSN: 2168-6262.

Li FD, Eagle S, Brophy C, Hocking KM, Osgood M, Komalavilas P, Cheung-Flynn J. Pressure control during preparation of saphenous veins. JAMA Surg. 2014 Jul; 149(7): 655-62. PMID: 24759942, PMCID: PMC4102634, PII: 1862292, DOI: 10.1001/jamasurg.2013.5067, ISSN: 2168-6262.

Osgood MJ, Hocking KM, Voskresensky IV, Li FD, Komalavilas P, Cheung-Flynn J, Brophy CM. Surgical vein graft preparation promotes cellular dysfunction, oxidative stress, and intimal hyperplasia in human saphenous vein. J Vasc Surg [print-electronic]. 2014 Jul; 60(1): 202-11. PMID: 23911244, PMCID: PMC3926896, PII: S0741-5214(13)01145-2, DOI: 10.1016/j.jvs.2013.06.004, ISSN: 1097-6809.

Li FD, Sexton KW, Hocking KM, Osgood MJ, Eagle S, Cheung-Flynn J, Brophy CM, Komalavilas P. Intimal thickness associated with endothelial dysfunction in human vein grafts. J Surg Res [print-electronic]. 2013 Mar; 180(1): e55-62. PMID: 22763213, PMCID: PMC3515722, PII: S0022-4804(12)00548-3, DOI: 10.1016/j.jss.2012.06.017, ISSN: 1095-8673.

Hocking KM, Baudenbacher FJ, Putumbaka G, Venkatraman S, Cheung-Flynn J, Brophy CM, Komalavilas P. Role of cyclic nucleotide-dependent actin cytoskeletal dynamics:Ca(2+)](i) and force suppression in forskolin-pretreated porcine coronary arteries. PLoS One [electronic-print]. 2013; 8(4): e60986. PMID: 23593369, PMCID: PMC3625185, PII: PONE-D-12-33807, DOI: 10.1371/journal.pone.0060986, ISSN: 1932-6203.

Osgood MJ, Harrison DG, Sexton KW, Hocking KM, Voskresensky IV, Komalavilas P, Cheung-Flynn J, Guzman RJ, Brophy CM. Role of the renin-angiotensin system in the pathogenesis of intimal hyperplasia: therapeutic potential for prevention of vein graft failure?. Ann Vasc Surg [print-electronic]. 2012 Nov; 26(8): 1130-44. PMID: 22445245, PMCID: PMC4189806, PII: S0890-5096(12)00052-0, DOI: 10.1016/j.avsg.2011.12.001, ISSN: 1615-5947.

Sexton KW, Hocking KM, Wise E, Osgood MJ, Cheung-Flynn J, Komalavilas P, Campbell KE, Dattilo JB, Brophy CM. Women in academic surgery: the pipeline is busted. J Surg Educ [print-electronic]. 2012 Jan; 69(1): 84-90. PMID: 22208838, PMCID: PMC3458116, PII: S1931-7204(11)00229-7, DOI: 10.1016/j.jsurg.2011.07.008, ISSN: 1878-7452.

Hartmann J, Wagner KV, Liebl C, Scharf SH, Wang XD, Wolf M, Hausch F, Rein T, Schmidt U, Touma C, Cheung-Flynn J, Cox MB, Smith DF, Holsboer F, Müller MB, Schmidt MV. The involvement of FK506-binding protein 51 (FKBP5) in the behavioral and neuroendocrine effects of chronic social defeat stress. Neuropharmacology [print-electronic]. 2012 Jan; 62(1): 332-9. PMID: 21839098, PII: S0028-3908(11)00329-7, DOI: 10.1016/j.neuropharm.2011.07.041, ISSN: 1873-7064.

Touma C, Gassen NC, Herrmann L, Cheung-Flynn J, Büll DR, Ionescu IA, Heinzmann JM, Knapman A, Siebertz A, Depping AM, Hartmann J, Hausch F, Schmidt MV, Holsboer F, Ising M, Cox MB, Schmidt U, Rein T. FK506 binding protein 5 shapes stress responsiveness: modulation of neuroendocrine reactivity and coping behavior. Biol. Psychiatry [print-electronic]. 2011 Nov 11/15/2011; 70(10): 928-36. PMID: 21907973, PII: S0006-3223(11)00767-0, DOI: 10.1016/j.biopsych.2011.07.023, ISSN: 1873-2402.

Eagle S, Brophy CM, Komalavilas P, Hocking K, Putumbaka G, Osgood M, Sexton K, Leacche M, Cheung-Flynn J. Surgical skin markers impair human saphenous vein graft smooth muscle and endothelial function. Am Surg. 2011 Jul; 77(7): 922-8. PMID: 21944360, PMCID: PMC3957273, ISSN: 1555-9823.

Hocking KM, Brophy C, Rizvi SZ, Komalavilas P, Eagle S, Leacche M, Balaguer JM, Cheung-Flynn J. Detrimental effects of mechanical stretch on smooth muscle function in saphenous veins. J. Vasc. Surg [print-electronic]. 2011 Feb; 53(2): 454-60. PMID: 21146345, PMCID: PMC3053010, PII: S0741-5214(10)02184-1, DOI: 10.1016/j.jvs.2010.09.010, ISSN: 1097-6809.

O'Leary JC, Dharia S, Blair LJ, Brady S, Johnson AG, Peters M, Cheung-Flynn J, Cox MB, de Erausquin G, Weeber EJ, Jinwal UK, Dickey CA. A new anti-depressive strategy for the elderly: ablation of FKBP5/FKBP51. PLoS ONE [print-electronic]. 2011; 6(9): e24840. PMID: 21935478, PMCID: PMC3174203, PII: PONE-D-11-12700, DOI: 10.1371/journal.pone.0024840, ISSN: 1932-6203.

Derkach DN, Wadekar SA, Perkins KB, Rousseau E, Dreiza CM, Cheung-Flynn J, Ramos HC, Ugarova TP, Sheller MR. RGD-dependent binding of TP508 to integrin alphavbeta3 mediates cell adhesion and induction of nitric oxide. Thromb. Haemost [print-electronic]. 2010 Jul; 104(1): 172-82. PMID: 20508901, PMCID: PMC4540356, PII: 09-07-0447, DOI: 10.1160/TH09-07-0447, ISSN: 0340-6245.

Flynn CR, Cheung-Flynn J, Smoke CC, Lowry D, Roberson R, Sheller MR, Brophy CM. Internalization and intracellular trafficking of a PTD-conjugated anti-fibrotic peptide, AZX100, in human dermal keloid fibroblasts. J Pharm Sci. 2010 Jul; 99(7): 3100-21. PMID: 20140957, DOI: 10.1002/jps.22087, ISSN: 1520-6017.

Tranguch S, Cheung-Flynn J, Daikoku T, Prapapanich V, Cox MB, Xie H, Wang H, Das SK, Smith DF, Dey SK. Cochaperone immunophilin FKBP52 is critical to uterine receptivity for embryo implantation. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2005 Oct 10/4/2005; 102(40): 14326-31. PMID: 16176985, PMCID: PMC1242310, PII: 0505775102, DOI: 10.1073/pnas.0505775102, ISSN: 0027-8424.

Cheung-Flynn J, Prapapanich V, Cox MB, Riggs DL, Suarez-Quian C, Smith DF. Physiological role for the cochaperone FKBP52 in androgen receptor signaling. Mol. Endocrinol [print-electronic]. 2005 Jun; 19(6): 1654-66. PMID: 15831525, PII: me.2005-0071, DOI: 10.1210/me.2005-0071, ISSN: 0888-8809.

Riggs DL, Cox MB, Cheung-Flynn J, Prapapanich V, Carrigan PE, Smith DF. Functional specificity of co-chaperone interactions with Hsp90 client proteins. Crit. Rev. Biochem. Mol. Biol. 2004 Sep; 39(5-6): 279-95. PMID: 15763706, PII: UA72K1EHB3YJ1QEJ, DOI: 10.1080/10409230490892513, ISSN: 1040-9238.

Hubler TR, Denny WB, Valentine DL, Cheung-Flynn J, Smith DF, Scammell JG. The FK506-binding immunophilin FKBP51 is transcriptionally regulated by progestin and attenuates progestin responsiveness. Endocrinology. 2003 Jun; 144(6): 2380-7. PMID: 12746298, DOI: 10.1210/en.2003-0092, ISSN: 0013-7227.

Cheung-Flynn J, Roberts PJ, Riggs DL, Smith DF. C-terminal sequences outside the tetratricopeptide repeat domain of FKBP51 and FKBP52 cause differential binding to Hsp90. J. Biol. Chem [print-electronic]. 2003 May 5/9/2003; 278(19): 17388-94. PMID: 12611898, PII: M300955200, DOI: 10.1074/jbc.M300955200, ISSN: 0021-9258.

Riggs DL, Roberts PJ, Chirillo SC, Cheung-Flynn J, Prapapanich V, Ratajczak T, Gaber R, Picard D, Smith DF. The Hsp90-binding peptidylprolyl isomerase FKBP52 potentiates glucocorticoid signaling in vivo. EMBO J. 2003 Mar 3/3/2003; 22(5): 1158-67. PMID: 12606580, PMCID: PMC150341, DOI: 10.1093/emboj/cdg108, ISSN: 0261-4189.

Sinars CR, Cheung-Flynn J, Rimerman RA, Scammell JG, Smith DF, Clardy J. Structure of the large FK506-binding protein FKBP51, an Hsp90-binding protein and a component of steroid receptor complexes. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2003 Feb 2/4/2003; 100(3): 868-73. PMID: 12538866, PMCID: PMC298693, PII: 0231020100, DOI: 10.1073/pnas.0231020100, ISSN: 0027-8424.

Cheung J, Smith DF. Molecular chaperone interactions with steroid receptors: an update. Mol. Endocrinol. 2000 Jul; 14(7): 939-46. PMID: 10894145, DOI: 10.1210/mend.14.7.0489, ISSN: 0888-8809.

Fajardo DA, Cheung J, Ito C, Sugawara E, Nikaido H, Misra R. Biochemistry and regulation of a novel Escherichia coli K-12 porin protein, OmpG, which produces unusually large channels. J. Bacteriol. 1998 Sep; 180(17): 4452-9. PMID: 9721282, PMCID: PMC107454, ISSN: 0021-9193.

Cheung J, Danna KJ, O'Connor EM, Price LB, Shand RF. Isolation, sequence, and expression of the gene encoding halocin H4, a bacteriocin from the halophilic archaeon Haloferax mediterranei R4. J. Bacteriol. 1997 Jan; 179(2): 548-51. PMID: 8990311, PMCID: PMC178729, ISSN: 0021-9193.