Heidi E. Hamm, Ph.D.



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Faculty Appointments
Professor of Pharmacology Professor of BiochemistryProfessor of Ophthalmology and Visual SciencesProfessor of Orthopaedic SurgeryAileen M. Lange and Annie Mary Lyle Chair in Cardiovascular Research
Ph.D., Zoology, University of Texas, Austin, TexasB.A., Languages, Atlantic Union College , South Lancaster, Massachusetts
Office Address
442 Robinson Research Building
23rd Avenue South @ Pierce
Nashville, TN 37232-6600
Research Description
My work is focused on understanding the molecular basis of signaling mechanisms mediated by G proteins, which are switch proteins. G proteins are normally inactive, but a receptor that has received a specific signal can activate G proteins, leading to changes in the activity of enzymes that produce second messengers such as cyclic AMP and calcium.

The resulting changes in cellular activity underlie a large number of physiological processes. G protein-mediated signalling cascades are key regulators of many physiological processes, including processes of development, differentiation, and regulation of cell division. In the brain, many key neurotransmitters and neuromodulators mediate a myriad of functions by activation of such G protein cascades.

The research in my laboratory is aimed at understanding how G proteins become activated by receptors, how they in turn activate effector enzymes, and how they turn off. We determined the sites of interaction between proteins using a method of decomposing the proteins into small synthetic peptides and determining which peptides blocked interaction sites (Hamm et al., 1988; Rarick et al., 1992; Artemyev et al., 1993; Arshavsky et al., 1994). To understand the process more fully, we determined the atomic structure of the proteins in collaboration with the group of Paul Sigler. We used X-ray crystallography to solve the three-dimensional structures of G proteins in their inactive (GDP bound, (Lambright et al., 1994) and activated (GTPbgS-bound) forms (Noel et al., 1993). We caught a glimpse of the self-inactivating process in another crystal form, the transition state analog, Ga.GDP.AlF4- (Sondek et al., 1994). More recently, the structures of the bg subunit (Sondek et al., 1996) and the heterotrimeric G protein (Lambright et al., 1996) were solved. These high-resolution structural studies allowed us to postulate specific hypotheses regarding mechanisms of receptor:G protein interaction and activation, G protein subunit association-dissociation and effector activation.

Lab URL: https://medschool.vanderbilt.edu/hamm-lab/
Research Keywords
Structure, function and interactions of G-protein coupled receptors, Molecular mechanisms of signal transduction, Vascular Biology
Duvernay MT, Temple KJ, Maeng JG, Blobaum AL, Stauffer SR, Lindsley CW, Hamm HE. Contributions of Protease-Activated Receptors PAR1 and PAR4 to Thrombin-Induced GPIIbIIIa Activation in Human Platelets. Mol. Pharmacol [print-electronic]. 2017 Jan; 91(1): 39-47. PMID: 27784794, PMCID: PMC5198515, PII: mol.116.106666, DOI: 10.1124/mol.116.106666, ISSN: 1521-0111.

Temple KJ, Duvernay MT, Maeng JG, Blobaum AL, Stauffer SR, Hamm HE, Lindsley CW. Identification of the minimum PAR4 inhibitor pharmacophore and optimization of a series of 2-methoxy-6-arylimidazo[2,1-b][1,3,4]thiadiazoles. Bioorg. Med. Chem. Lett [print-electronic]. 2016 Nov 11/15/2016; 26(22): 5481-6. PMID: 27777004, PMCID: PMC5340293, PII: S0960-894X(16)31048-4, DOI: 10.1016/j.bmcl.2016.10.020, ISSN: 1464-3405.

Oliver KH, Duvernay MT, Hamm HE, Carneiro AM. Loss of Serotonin Transporter Function Alters ADP-mediated Glycoprotein aIIbß3 Activation through Dysregulation of the 5-HT2A Receptor. J. Biol. Chem [print-electronic]. 2016 Sep 9/16/2016; 291(38): 20210-9. PMID: 27422820, PMCID: PMC5025703, PII: M116.736983, DOI: 10.1074/jbc.M116.736983, ISSN: 1083-351X.

Kaya AI, Lokits AD, Gilbert JA, Iverson TM, Meiler J, Hamm HE. A Conserved Hydrophobic Core in Gai1 Regulates G Protein Activation and Release from Activated Receptor. J. Biol. Chem [print-electronic]. 2016 Sep 9/9/2016; 291(37): 19674-86. PMID: 27462082, PMCID: PMC5016700, PII: M116.745513, DOI: 10.1074/jbc.M116.745513, ISSN: 1083-351X.

Temple KJ, Duvernay MT, Young SE, Wen W, Wu W, Maeng JG, Blobaum AL, Stauffer SR, Hamm HE, Lindsley CW. Development of a Series of (1-Benzyl-3-(6-methoxypyrimidin-3-yl)-5-(trifluoromethoxy)-1H-indol-2-yl)methanols as Selective Protease Activated Receptor 4 (PAR4) Antagonists with in Vivo Utility and Activity Against ¿-Thrombin. J. Med. Chem [print-electronic]. 2016 Aug 8/25/2016; 59(16): 7690-5. PMID: 27482618, PMCID: PMC5775816, DOI: 10.1021/acs.jmedchem.6b00928, ISSN: 1520-4804.

Gupta A, Gomes I, Bobeck EN, Fakira AK, Massaro NP, Sharma I, Cavé A, Hamm HE, Parello J, Devi LA. Collybolide is a novel biased agonist of ¿-opioid receptors with potent antipruritic activity. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2016 May 5/24/2016; 113(21): 6041-6. PMID: 27162327, PMCID: PMC4889365, PII: 1521825113, DOI: 10.1073/pnas.1521825113, ISSN: 1091-6490.

Friedman EA, Texeira L, Delaney J, Weeke PE, Lynch DR, Kasasbeh E, Song Y, Harrell FE, Denny JC, Hamm HE, Roden DM, Cleator JH. Evaluation of the F2R IVS-14A/T PAR1 polymorphism with subsequent cardiovascular events and bleeding in patients who have undergone percutaneous coronary intervention. J. Thromb. Thrombolysis. 2016 May; 41(4): 656-62. PMID: 26446588, PII: 10.1007/s11239-015-1285-4, DOI: 10.1007/s11239-015-1285-4, ISSN: 1573-742X.

Zurawski Z, Rodriguez S, Hyde K, Alford S, Hamm HE. Gß¿ Binds to the Extreme C Terminus of SNAP25 to Mediate the Action of Gi/o-Coupled G Protein-Coupled Receptors. Mol. Pharmacol [print-electronic]. 2016 Jan; 89(1): 75-83. PMID: 26519224, PMCID: PMC4702098, PII: mol.115.101600, DOI: 10.1124/mol.115.101600, ISSN: 1521-0111.

Duvernay MT, Matafonov A, Lindsley CW, Hamm HE. Platelet Lipidomic Profiling: Novel Insight into Cytosolic Phospholipase A2a Activity and Its Role in Human Platelet Activation. Biochemistry [print-electronic]. 2015 Sep 9/15/2015; 54(36): 5578-88. PMID: 26295742, DOI: 10.1021/acs.biochem.5b00549, ISSN: 1520-4995.

Yim YY, Betke K, Hamm H. Using peptide arrays created by the SPOT method for defining protein-protein interactions. Methods Mol. Biol. 2015; 1278: 307-20. PMID: 25859957, DOI: 10.1007/978-1-4939-2425-7_18, ISSN: 1940-6029.

Kaya AI, Iverson TM, Hamm HE. Functional stability of rhodopsin in a bicelle system: evaluating G protein activation by rhodopsin in bicelles. Methods Mol. Biol. 2015; 1271: 67-76. PMID: 25697517, DOI: 10.1007/978-1-4939-2330-4_5, ISSN: 1940-6029.

Jacobson R, Mignemi N, Rose K, O'Rear L, Sarilla S, Hamm HE, Barnett JV, Verhamme IM, Schoenecker J. The hyperglycemic byproduct methylglyoxal impairs anticoagulant activity through covalent adduction of antithrombin III. Thromb. Res [print-electronic]. 2014 Dec; 134(6): 1350-7. PMID: 25307422, PMCID: PMC4337957, PII: S0049-3848(14)00523-4, DOI: 10.1016/j.thromres.2014.09.038, ISSN: 1879-2472.

Wen W, Young SE, Duvernay MT, Schulte ML, Nance KD, Melancon BJ, Engers J, Locuson CW, Wood MR, Daniels JS, Wu W, Lindsley CW, Hamm HE, Stauffer SR. Substituted indoles as selective protease activated receptor 4 (PAR-4) antagonists: Discovery and SAR of ML354. Bioorg. Med. Chem. Lett [print-electronic]. 2014 Oct 10/1/2014; 24(19): 4708-13. PMID: 25176330, PMCID: PMC5716344, PII: S0960-894X(14)00852-X, DOI: 10.1016/j.bmcl.2014.08.021, ISSN: 1464-3405.

Cleator JH, Duvernay MT, Holinstat M, Colowick NE, Hudson WJ, Song Y, Harrell FE, Hamm HE. Racial differences in resistance to P2Y12 receptor antagonists in type 2 diabetic subjects. J. Pharmacol. Exp. Ther [print-electronic]. 2014 Oct; 351(1): 33-43. PMID: 25052834, PMCID: PMC4165026, PII: jpet.114.215616, DOI: 10.1124/jpet.114.215616, ISSN: 1521-0103.

Kaya AI, Lokits AD, Gilbert JA, Iverson TM, Meiler J, Hamm HE. A conserved phenylalanine as a relay between the a5 helix and the GDP binding region of heterotrimeric Gi protein a subunit. J. Biol. Chem [print-electronic]. 2014 Aug 8/29/2014; 289(35): 24475-87. PMID: 25037222, PMCID: PMC4148873, PII: M114.572875, DOI: 10.1074/jbc.M114.572875, ISSN: 1083-351X.

Betke KM, Rose KL, Friedman DB, Baucum AJ, Hyde K, Schey KL, Hamm HE. Differential localization of G protein ß¿ subunits. Biochemistry [print-electronic]. 2014 Apr 4/15/2014; 53(14): 2329-43. PMID: 24568373, PMCID: PMC4004276, DOI: 10.1021/bi500091p, ISSN: 1520-4995.

Alexander NS, Preininger AM, Kaya AI, Stein RA, Hamm HE, Meiler J. Energetic analysis of the rhodopsin-G-protein complex links the a5 helix to GDP release. Nat. Struct. Mol. Biol [print-electronic]. 2014 Jan; 21(1): 56-63. PMID: 24292645, PMCID: PMC3947367, PII: nsmb.2705, DOI: 10.1038/nsmb.2705, ISSN: 1545-9985.

Hamid E, Church E, Wells CA, Zurawski Z, Hamm HE, Alford S. Modulation of neurotransmission by GPCRs is dependent upon the microarchitecture of the primed vesicle complex. J. Neurosci. 2014 Jan 1/1/2014; 34(1): 260-74. PMID: 24381287, PMCID: PMC3866488, PII: 34/1/260, DOI: 10.1523/JNEUROSCI.3633-12.2014, ISSN: 1529-2401.

Hamm HE, Kaya AI, Gilbert JA, Preininger AM. Linking receptor activation to changes in Sw I and II of Ga proteins. J. Struct. Biol [print-electronic]. 2013 Oct; 184(1): 63-74. PMID: 23466875, PMCID: PMC3726552, PII: S1047-8477(13)00053-1, DOI: 10.1016/j.jsb.2013.02.016, ISSN: 1095-8657.

Preininger AM, Meiler J, Hamm HE. Conformational flexibility and structural dynamics in GPCR-mediated G protein activation: a perspective. J. Mol. Biol [print-electronic]. 2013 Jul 7/10/2013; 425(13): 2288-98. PMID: 23602809, PMCID: PMC3686903, PII: S0022-2836(13)00242-8, DOI: 10.1016/j.jmb.2013.04.011, ISSN: 1089-8638.

Li X, Roszko I, Sepich DS, Ni M, Hamm HE, Marlow FL, Solnica-Krezel L. Gpr125 modulates Dishevelled distribution and planar cell polarity signaling. Development. 2013 Jul; 140(14): 3028-39. PMID: 23821037, PMCID: PMC3699285, PII: 140/14/3028, DOI: 10.1242/dev.094839, ISSN: 1477-9129.

Duvernay M, Young S, Gailani D, Schoenecker J, Hamm HE. Protease-activated receptor (PAR) 1 and PAR4 differentially regulate factor V expression from human platelets. Mol. Pharmacol [print-electronic]. 2013 Apr; 83(4): 781-92. PMID: 23307185, PMCID: PMC3608438, PII: mol.112.083477, DOI: 10.1124/mol.112.083477, ISSN: 1521-0111.

Natarajan C, Hata AN, Hamm HE, Zent R, Breyer RM. Extracellular loop II modulates GTP sensitivity of the prostaglandin EP3 receptor. Mol. Pharmacol [print-electronic]. 2013 Jan; 83(1): 206-16. PMID: 23087260, PMCID: PMC3533480, PII: mol.112.080473, DOI: 10.1124/mol.112.080473, ISSN: 1521-0111.

Young SE, Duvernay MT, Schulte ML, Lindsley CW, Hamm HE. Synthesis of indole derived protease-activated receptor 4 antagonists and characterization in human platelets. PLoS ONE [electronic-print]. 2013; 8(6): e65528. PMID: 23776495, PMCID: PMC3679140, PII: PONE-D-13-05376, DOI: 10.1371/journal.pone.0065528, ISSN: 1932-6203.

Wells CA, Zurawski Z, Betke KM, Yim YY, Hyde K, Rodriguez S, Alford S, Hamm HE. Gß¿ inhibits exocytosis via interaction with critical residues on soluble N-ethylmaleimide-sensitive factor attachment protein-25. Mol. Pharmacol [print-electronic]. 2012 Dec; 82(6): 1136-49. PMID: 22962332, PMCID: PMC3502621, PII: mol.112.080507, DOI: 10.1124/mol.112.080507, ISSN: 1521-0111.

Ichikawa J, Cole HA, Magnussen RA, Mignemi NA, Butler M, Holt GE, O'Rear L, Yuasa M, Pabla B, Haro H, Cates JM, Hamm HE, Schwartz HS, Schoenecker JG. Thrombin induces osteosarcoma growth, a function inhibited by low molecular weight heparin in vitro and in vivo: procoagulant nature of osteosarcoma. Cancer [print-electronic]. 2012 May 5/1/2012; 118(9): 2494-506. PMID: 21953059, DOI: 10.1002/cncr.26518, ISSN: 1097-0142.

Preininger AM, Kaya AI, Gilbert JA, Busenlehner LS, Armstrong RN, Hamm HE. Myristoylation exerts direct and allosteric effects on Ga conformation and dynamics in solution. Biochemistry [print-electronic]. 2012 Mar 3/6/2012; 51(9): 1911-24. PMID: 22329346, PMCID: PMC3312377, DOI: 10.1021/bi201472c, ISSN: 1520-4995.

Betke KM, Wells CA, Hamm HE. GPCR mediated regulation of synaptic transmission. Prog. Neurobiol [print-electronic]. 2012 Mar; 96(3): 304-21. PMID: 22307060, PMCID: PMC3319362, PII: S0301-0082(12)00010-X, DOI: 10.1016/j.pneurobio.2012.01.009, ISSN: 1873-5118.

Wells CA, Betke KM, Lindsley CW, Hamm HE. Label-free detection of G protein-SNARE interactions and screening for small molecule modulators. ACS Chem Neurosci. 2012 Jan 1/18/2012; 3(1): 69-78. PMID: 22368765, PMCID: PMC3285104, DOI: 10.1021/cn200102d, ISSN: 1948-7193.

Thaker TM, Kaya AI, Preininger AM, Hamm HE, Iverson TM. Allosteric mechanisms of G protein-Coupled Receptor signaling: a structural perspective. Methods Mol. Biol. 2012; 796: 133-74. PMID: 22052489, PMCID: PMC3549666, DOI: 10.1007/978-1-61779-334-9_8, ISSN: 1940-6029.

Kaya AI, Thaker TM, Preininger AM, Iverson TM, Hamm HE. Coupling efficiency of rhodopsin and transducin in bicelles. Biochemistry [print-electronic]. 2011 Apr 4/19/2011; 50(15): 3193-203. PMID: 21375271, PMCID: PMC3119548, DOI: 10.1021/bi200037j, ISSN: 1520-4995.

Lenoci L, Duvernay M, Satchell S, DiBenedetto E, Hamm HE. Mathematical model of PAR1-mediated activation of human platelets. Mol Biosyst [print-electronic]. 2011 Apr; 7(4): 1129-37. PMID: 21229145, DOI: 10.1039/c0mb00250j, ISSN: 1742-2051.

Van Eps, N, AM Preininger, N Alexander, AI Kaya, S Meier, J Meiler, HE Hamm, WL Hubbell. Interaction of a G protein with an activated receptor opens the interdomain interface in the alpha subunit. PNAS USA. 2011; 108((23)): 9420-4.

Caruso G, Bisegna P, Andreucci D, Lenoci L, Gurevich VV, Hamm HE, DiBenedetto E. Identification of key factors that reduce the variability of the single photon response. PNAS USA. 2011; 108((19)): 7804-7.

Holinstat M, Boutaud O, Apopa PL, Vesci J, Bala M, Oates JA, Hamm HE. Protease-activated receptor signaling in platelets activates cytosolic phospholipase A2α differently for cyclooxygenase-1 and 12-lipoxygenase catalysis. Arterioscler Thromb Vasc Biol. 2011; 31((2)): 435-42.

Shen L, Caruso G, Bisegna P, Andreucci D, Gurevich VV, Hamm HE, DiBenedetto E. Dynamics of mouse rod phototransduction and its sensitivity to variation of key parameters. IET Syst Biol. 2010 Jan; 4(1): 12-32. PMID: 20001089, PMCID: PMC3833298, DOI: 10.1049/iet-syb.2008.0154, ISSN: 1751-8849.

Jernigan KK, Cselenyi CS, Thorne CA, Hanson AJ, Tahinci E, Hajicek N, Oldham WM, Lee LA, Hamm HE, Hepler JR, Kozasa T, Linder ME, Lee E. Gbetagamma activates GSK3 to promote LRP6-mediated beta-catenin transcriptional activity. Sci Signal. 2010; 3(121): ra37. PMID: 20460648, PMCID: PMC3088111, PII: 3/121/ra37, DOI: 10.1126/scisignal.2000647, ISSN: 1937-9145.

Caruso G, Bisegna P, Lenoci L, Andreucci D, Gurevich VV, Hamm HE, DiBenedetto E. Kinetics of rhodopsin deactivation and its role in regulating recovery and reproducibility of rod photoresponse. PLoS Comput Biol. 2010; 6((12)): e1001031.

Marjoram RJ, Voss B, Pan Y, Dickeson SK, Zutter MM, Hamm HE, Santoro SA. Suboptimal activation of protease-activated receptors enhances alpha2beta1 integrin-mediated platelet adhesion to collagen. J. Biol. Chem [print-electronic]. 2009 Dec 12/11/2009; 284(50): 34640-7. PMID: 19815553, PMCID: PMC2787326, PII: M109.020990, DOI: 10.1074/jbc.M109.020990, ISSN: 1083-351X.

Hamm HE, Meier SM, Liao G, Preininger AM. Trp fluorescence reveals an activation-dependent cation-pi interaction in the Switch II region of Galphai proteins. Protein Sci. 2009 Nov; 18(11): 2326-35. PMID: 19760664, PMCID: PMC2788287, DOI: 10.1002/pro.243, ISSN: 1469-896X.

Holinstat M, Preininger AM, Milne SB, Hudson WJ, Brown HA, Hamm HE. Irreversible platelet activation requires protease-activated receptor 1-mediated signaling to phosphatidylinositol phosphates. Mol. Pharmacol [print-electronic]. 2009 Aug; 76(2): 301-13. PMID: 19483102, PMCID: PMC2713123, PII: mol.109.056622, DOI: 10.1124/mol.109.056622, ISSN: 1521-0111.

Preininger AM, Funk MA, Oldham WM, Meier SM, Johnston CA, Adhikary S, Kimple AJ, Siderovski DP, Hamm HE, Iverson TM. Helix dipole movement and conformational variability contribute to allosteric GDP release in Galphai subunits. Biochemistry. 2009 Mar 3/31/2009; 48(12): 2630-42. PMID: 19222191, PMCID: PMC2736342, PII: 10.1021/bi801853a, DOI: 10.1021/bi801853a, ISSN: 1520-4995.

Lin F, Chen S, Sepich DS, Panizzi JR, Clendenon SG, Marrs JA, Hamm HE, Solnica-Krezel L. Galpha12/13 regulate epiboly by inhibiting E-cadherin activity and modulating the actin cytoskeleton. J. Cell Biol. 2009 Mar 3/23/2009; 184(6): 909-21. PMID: 19307601, PMCID: PMC2664974, PII: jcb.200805148, DOI: 10.1083/jcb.200805148, ISSN: 1540-8140.

Marjoram RJ, Voss B, Pan Y, Dickeson SK, Zutter MM, Hamm HE, and Santoro, SA. Suboptimal Activation of Protease-activated Receptors Enhances alpha2beta1 Integrin-mediated Platelet Adhesion to Collagen. J Biol Chem. 2009; 284((50)): 34640-7.

Preininger AM, Funk MA, Oldham WM, Meier SM, Johnston CA, Adhikary S, Kimple AJ, Siderovski DP, Hamm HE, Iverson TM. Helix dipole movement and conformational variability contribute to allosteric GDP release in Galphai subunits. Biochemistry. 2009; 48((12)): 2630-42.

Lin F, Chen S, Sepich DS, Panizzi JR, Clendenon SG, Marrs JA, Hamm HE, Solnica-Krezel L. Galpha 12/13 regulate epiboly by inhibiting E-cadherin activity and modulating the actin cytoskeleton. J Cell Biol. 2009; 184((6)): 909-21.

Hamm HE, Meier SM, Liao G, Preininger AM. Trp fluorescence reveals an activation-dependent cation-pi interaction in the Switch II region of Galphai proteins. Protein Sci. 2009; 18((11)): 2326-35.

Mazzoni MR, Porchia F, Hamm HE. Proteolytic fragmentation for epitope mapping. Methods Mol Biol. 2009; 524: 77-86.

Wen XH, Shen L, Brush RS, Michaud N, Al-Ubaidi MR, Gurevich VV, Hamm HE, Lem J, Dibenedetto E, Anderson RE, Makino CL. Overexpression of rhodopsin alters the structure and photoresponse of rod photoreceptors. Biophys J. 2009; 96((3)): 939-50.

Preininger, AM, Funk, MA, Oldham, WM, Meier, SM, Johnston, CA, Adhikary, S, Kimple, AJ, Siderovski, DP, Hamm, HE, Iverson, TM. Helix Dipole Movement and Conformational Variability Contribute to Allosteric GDP Release in Galphai Subunits. Biochemistry. 2009; 48((12)): 2630-42.

Yoon EJ, Hamm HE, Currie KP. G protein betagamma subunits modulate the number and nature of exocytotic fusion events in adrenal chromaffin cells independent of calcium entry. J. Neurophysiol [print-electronic]. 2008 Nov; 100(5): 2929-39. PMID: 18815342, PMCID: PMC2585407, PII: 90839.2008, DOI: 10.1152/jn.90839.2008, ISSN: 0022-3077.

Preininger AM, Parello J, Meier SM, Liao G, Hamm HE. Receptor-mediated changes at the myristoylated amino terminus of Galpha(il) proteins. Biochemistry [print-electronic]. 2008 Sep 9/30/2008; 47(39): 10281-93. PMID: 18771287, PMCID: PMC2751642, DOI: 10.1021/bi800741r, ISSN: 1520-4995.

Chen S, Lin F, Shin ME, Wang F, Shen L, Hamm HE. RACK1 regulates directional cell migration by acting on G betagamma at the interface with its effectors PLC beta and PI3K gamma. Mol. Biol. Cell [print-electronic]. 2008 Sep; 19(9): 3909-22. PMID: 18596232, PMCID: PMC2526680, PII: E08-04-0433, DOI: 10.1091/mbc.E08-04-0433, ISSN: 1939-4586.

Oldham WM, Hamm HE. Heterotrimeric G protein activation by G-protein-coupled receptors. Nat. Rev. Mol. Cell Biol. 2008 Jan; 9(1): 60-71. PMID: 18043707, PII: nrm2299, DOI: 10.1038/nrm2299, ISSN: 1471-0080.

Preininger AM, Parello J, Meier SM, Liao G, Hamm HE. Receptor-Mediated Changes at the Myristoylated Amino Terminus of Galpha(il) Proteins. Biochemistry. 2008; 47((39)): 10281-93.

Chen S, Lin F, Shin ME, Wang F, Shen L, Hamm HE. RACK1 Regulates Directional Cell Migration by Acting on Gbetagamma at the Interface with Its Effectors PLCbeta and P13Kgamma. Mol Biol Cell. 2008; 19((9)): 3909-22.

Bisegna P, Caruso G, Andreucci D, Shen L, Gurevich VV, Hamm HE, DiBenedetto E. Diffusion of the second messengers in the cytoplasm acts as a variability suppressor of the single photon response in vertebrate phototransduction. Biophys J. 2008; 94((9)): 3363-83.

Yoon, EJ, Hamm, HE, Currie, KP. G protein betagamma Subunits Modulate the Number and Nature of Exocytotic Fusion Events in Adrenal Chromaffin Cells Independent of Calcium Entry. J Neurophysiol. 2008; 100((5)): 2929-39.

Yoon EJ, Gerachshenko T, Spiegelberg BD, Alford S, Hamm HE. Gbetagamma interferes with Ca2+-dependent binding of synaptotagmin to the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex. Mol. Pharmacol [print-electronic]. 2007 Nov; 72(5): 1210-9. PMID: 17715396, PII: mol.107.039446, DOI: 10.1124/mol.107.039446, ISSN: 0026-895X.

Bilodeau ML, Hamm HE. Regulation of protease-activated receptor (PAR) 1 and PAR4 signaling in human platelets by compartmentalized cyclic nucleotide actions. J. Pharmacol. Exp. Ther [print-electronic]. 2007 Aug; 322(2): 778-88. PMID: 17525299, PII: jpet.107.121830, DOI: 10.1124/jpet.107.121830, ISSN: 0022-3565.

Oldham WM, Van Eps N, Preininger AM, Hubbell WL, Hamm HE. Mapping allosteric connections from the receptor to the nucleotide-binding pocket of heterotrimeric G proteins. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2007 May 5/8/2007; 104(19): 7927-32. PMID: 17463080, PMCID: PMC1876549, PII: 0702623104, DOI: 10.1073/pnas.0702623104, ISSN: 0027-8424.

Voss B, McLaughlin JN, Holinstat M, Zent R, Hamm HE. PAR1, but not PAR4, activates human platelets through a Gi/o/phosphoinositide-3 kinase signaling axis. Mol. Pharmacol [print-electronic]. 2007 May; 71(5): 1399-406. PMID: 17303701, PII: mol.106.033365, DOI: 10.1124/mol.106.033365, ISSN: 0026-895X.

Huang K, Voss B, Kumar D, Hamm HE, Harth E. Dendritic molecular transporters provide control of delivery to intracellular compartments. Bioconjug. Chem [print-electronic]. 2007 Mar; 18(2): 403-9. PMID: 17284011, DOI: 10.1021/bc060287a, ISSN: 1043-1802.

Holinstat M, Voss B, Bilodeau ML, Hamm HE. Protease-activated receptors differentially regulate human platelet activation through a phosphatidic acid-dependent pathway. Mol. Pharmacol [print-electronic]. 2007 Mar; 71(3): 686-94. PMID: 17151288, PII: mol.106.029371, DOI: 10.1124/mol.106.029371, ISSN: 0026-895X.

Spiegelberg BD, Hamm HE. Roles of G-protein-coupled receptor signaling in cancer biology and gene transcription. Curr. Opin. Genet. Dev [print-electronic]. 2007 Feb; 17(1): 40-4. PMID: 17188489, PII: S0959-437X(06)00237-1, DOI: 10.1016/j.gde.2006.12.002, ISSN: 0959-437X.

Oldham WM, Hamm HE. How do receptors activate G proteins?. Adv. Protein Chem. 2007; 74: 67-93. PMID: 17854655, PII: S0065-3233(07)74002-0, DOI: 10.1016/S0065-3233(07)74002-0, ISSN: 0065-3233.

Yoon EJ, Gerachshenko T, Spiegelberg BD, Alford S, Hamm HE. Gbetagamma Interferes with Ca2+-Dependent Binding of Synaptotagmin to the Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptor (SNARE) Complex. Mol Pharmacol. 2007; 72((5)): 1210-9.

Oldham WM, Van Eps N, Preininger AM, Hubbell WL, Hamm HE. Mapping allosteric connections from the receptor to the nucleotide-binding pocket of heterotrimeric G proteins. Proc Natl Acad Sci USA. 2007; 104((19)): 7927-32.

Chen S, Hamm HE. DEP domains: More than just membrane anchors. Dev. Cell. 2006 Oct; 11(4): 436-8. PMID: 17011483, PII: S1534-5807(06)00404-7, DOI: 10.1016/j.devcel.2006.09.011, ISSN: 1534-5807.

Holinstat M, Voss B, Bilodeau ML, McLaughlin JN, Cleator J, Hamm HE. PAR4, but not PAR1, signals human platelet aggregation via Ca2+ mobilization and synergistic P2Y12 receptor activation. J. Biol. Chem [print-electronic]. 2006 Sep 9/8/2006; 281(36): 26665-74. PMID: 16837456, PMCID: PMC3035573, PII: M602174200, DOI: 10.1074/jbc.M602174200, ISSN: 0021-9258.

Oldham WM, Van Eps N, Preininger AM, Hubbell WL, Hamm HE. Mechanism of the receptor-catalyzed activation of heterotrimeric G proteins. Nat. Struct. Mol. Biol [print-electronic]. 2006 Sep; 13(9): 772-7. PMID: 16892066, PII: nsmb1129, DOI: 10.1038/nsmb1129, ISSN: 1545-9993.

Ding J, Guzman JN, Tkatch T, Chen S, Goldberg JA, Ebert PJ, Levitt P, Wilson CJ, Hamm HE, Surmeier DJ. RGS4-dependent attenuation of M4 autoreceptor function in striatal cholinergic interneurons following dopamine depletion. Nat. Neurosci [print-electronic]. 2006 Jun; 9(6): 832-42. PMID: 16699510, PII: nn1700, DOI: 10.1038/nn1700, ISSN: 1097-6256.

Oldham WM, Hamm HE. Structural basis of function in heterotrimeric G proteins. Q. Rev. Biophys [print-electronic]. 2006 May; 39(2): 117-66. PMID: 16923326, PII: S0033583506004306, DOI: 10.1017/S0033583506004306, ISSN: 0033-5835.

Bilodeau ML, Hamm HE. Endothelial nitric-oxide synthase reveals a new face in G protein signaling. Mol. Pharmacol [print-electronic]. 2006 Mar; 69(3): 677-9. PMID: 16377762, PII: mol.105.022038, DOI: 10.1124/mol.105.022038, ISSN: 0026-895X.

Murphy SC, Harrison T, Hamm HE, Lomasney JW, Mohandas N, Haldar K. Erythrocyte G protein as a novel target for malarial chemotherapy. PLoS Med. 2006; 3((12)): e528.

Van Eps N, Oldham WM, Hamm HE, Hubbell WL. Structural and dynamical changes in an alpha-subunit of a heterotrimeric G protein along the activation pathway. Proc Natl Acad Sci USA. 2006; 103((44)): 16194-9.

Preininger AM, Henage LG, Oldham WM, Yoon EJ, Hamm HE, Brown HA. Direct Modulation of Phospholipase D Activity by Gbetagamma. Mol Pharmacol. 2006; 70((1)): 311-8.

Caruso G, Bisegna P, Shen L, Andreucci D, Hamm HE, DiBenedetto E. Modeling the role of incisures in vertebrate phototransduction. Biophys J. 2006; 91((4)): 1192-212.

Georgoussi Z, Leontiadis L, Mazarakou G, Merkouris M, Hyde K, Hamm H. Selective interactions between G protein subunits and RGS4 with the C-terminal domains of the mu- and delta-opiod receptors regulate opioid receptor signaling. Cell Signal. 2006; 18((6)): 771-82.

Cleator JH, Zhu WQ, Vaughan DE, Hamm HE. Differential regulation of endothelial exocytosis of P-selectin and von Willebrand factor by protease-activated receptors and cAMP. Blood. 2006; 107((7)): 2736-44.

Photowala H, Blackmer T, Schwartz E, Hamm HE, Alford S. G protein betagamma-subunits activated by serotonin mediate presynaptic inhibition by regulating vesicle fusion properties. Proc Natl Acad Sci USA. 2006; 103((11)): 4281-6.

Holinstat M, Oldham WM, Hamm HE. G-protein-coupled receptors: evolving views on physiological signalling: symposium on G-protein-coupled receptors: evolving concepts and new techniques. EMBO Rep. 2006; 7((9)): 866-9.

Caruso G, Khanal H, Alexiades V, Rieke F, Hamm HE, DiBenedetto E. Mathematical and computational modelling of spatio-temporal signalling in rod phototransduction. Syst Biol (Stevenage). 2005 Sep; 152(3): 119-37. PMID: 16986276, ISSN: 1741-2471.

McLaughlin JN, Shen L, Holinstat M, Brooks JD, Dibenedetto E, Hamm HE. Functional selectivity of G protein signaling by agonist peptides and thrombin for the protease-activated receptor-1. J. Biol. Chem [print-electronic]. 2005 Jul 7/1/2005; 280(26): 25048-59. PMID: 15878870, PII: M414090200, DOI: 10.1074/jbc.M414090200, ISSN: 0021-9258.

McLaughlin JN, Mazzoni MR, Cleator JH, Earls L, Perdigoto AL, Brooks JD, Muldowney JA, Vaughan DE, Hamm HE. Thrombin modulates the expression of a set of genes including thrombospondin-1 in human microvascular endothelial cells. J. Biol. Chem [print-electronic]. 2005 Jun 6/10/2005; 280(23): 22172-80. PMID: 15817447, PII: M500721200, DOI: 10.1074/jbc.M500721200, ISSN: 0021-9258.

Spiegelberg BD, Hamm HE. Gbetagamma Binds Histone Deacetylase 5 (HDAC5) and Inhibits Its Transcriptional Co-repression Activity. J Biol Chem. 2005; 280((50)): 41769-76.

Chen S, Lin F, Hamm HE. RACK1 Binds to a Signal Transfer Region of Gbetagamma and Inhibits Phospholipase C beta2 activation. J Biol Chem. 2005; 280((39)): 33445-52.

Lin F, Sepich DS, Chen S, Topczewski J, Yin C, Solnica-Krezel L, Hamm H. Essential roles of Galpha 12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements. J Cell Biol. 2005; 169((5)): 777-87.

Blackmer T, Larsen EC, Bartleson C, Kowalchyk JA, Yoon EJ, Preininger AM, Alford S, Hamm HE, Martin TF. G protein betagamma directly regulates SNARE protein fusion machinery for secretory granule exocytosis. Nat Neurosci. 2005; 8((4)): 421-5.

Gerachshenko T, Blackmer T, Yoon EJ, Bartleson C, Hamm HE, Alford S. Gbetagamma acts at the C terminus of SNAP-25 to mediate presynaptic inhibition. Nat Neurosci. 2005; 8((5)): 597-605.

Lukov GL, Hu T, McLaughlin JN, Hamm HE, Willardson BM. Phosducin-like protein acts as a molecular chaperone for G protein betagamma dimer assembly. EMBO J. 2005; 24((11)): 1965-75.

Chen S, Spiegelberg BD, Lin F, Dell EJ, Hamm HE. Interaction of Gbetagamma with RACK1 and other WD40 repeat proteins. J. Mol. Cell. Cardiol. 2004 Aug; 37(2): 399-406. PMID: 15276010, PII: S0022282804001373, DOI: 10.1016/j.yjmcc.2004.04.019, ISSN: 0022-2828.

Srinivasan C, Toon J, Amari L, Abukhdeir AM, Hamm H, Geraldes CF, Ho YK, Mota de Freitas D. Competition between lithium and magnesium ions for the G-protein transducin in the guanosine 5'-diphosphate bound conformation. J. Inorg. Biochem. 2004 May; 98(5): 691-701. PMID: 15134914, PII: S0162013404000054, DOI: 10.1016/j.jinorgbio.2003.12.023, ISSN: 0162-0134.

Preininger AM, Hamm HE. G protein signaling: insights from new structures. Sci. STKE. 2004 Feb 2/3/2004; 2004(218): re3. PMID: 14762218, PII: stke.2182004re3, DOI: 10.1126/stke.2182004re3, ISSN: 1525-8882.

Cabrera-Vera TM, Hernandez S, Earls LR, Medkova M, Sundgren-Andersson AK, Surmeier DJ, Hamm HE. RGS9-2 modulates D2 dopamine receptor-mediated Ca2+ channel inhibition in rat striatal cholinergic interneurons. Proc Natl Acad Sci USA. 2004; 16((46)): 16339-44.

Chen S, Dell EJ, Lin F, Sai J, Hamm HE. RACK1 Regulates Specific Functions of Gbetagamma. J Bio Chem. 2004; 279((17)): 17861-8.

Slessareva JE, Ma H, Depree KM, Flood LA, Bae H, Cabrera-Vera TM, Hamm HE, Graber SG. Closely related G-protein-coupled receptors use multiple and distinct domains on G-protein alpha-subunits for selective coupling. J. Biol. Chem [print-electronic]. 2003 Dec 12/12/2003; 278(50): 50530-6. PMID: 14525988, PII: M304417200, DOI: 10.1074/jbc.M304417200, ISSN: 0021-9258.

Cabrera-Vera TM, Vanhauwe J, Thomas TO, Medkova M, Preininger A, Mazzoni MR, Hamm HE. Insights into G protein structure, function, and regulation. Endocr. Rev. 2003 Dec; 24(6): 765-81. PMID: 14671004, DOI: 10.1210/er.2000-0026, ISSN: 0163-769X.

Harrison T, Samuel BU, Akompong T, Hamm H, Mohandas N, Lomasney JW, Haldar K. Erythrocyte G protein-coupled receptor signaling in malarial infection. Science. 2003 Sep 9/19/2003; 301(5640): 1734-6. PMID: 14500986, PII: 301/5640/1734, DOI: 10.1126/science.1089324, ISSN: 1095-9203.

Preininger AM, Van Eps N, Yu NJ, Medkova M, Hubbell WL, Hamm HE. The myristoylated amino terminus of Galpha(i)(1) plays a critical role in the structure and function of Galpha(i)(1) subunits in solution. Biochemistry. 2003 Jul 7/8/2003; 42(26): 7931-41. PMID: 12834345, DOI: 10.1021/bi0345438, ISSN: 0006-2960.

Andreucci D, Bisegna P, Caruso G, Hamm HE, DiBenedetto E. Mathematical model of the spatio-temporal dynamics of second messengers in visual transduction. Biophys J. 2003; 85((3)): 1358-76.

Chen NF, Yu JZ, Skiba NP, Hamm HE, Rasenick MM. A Specific Domain of Gialpha Required for the Transactivation of Gialpha by Tubulin Is Implicated in the Organization of Cellular Microtubules. J Biol Chem. 2003; 278((17)): 15285-90.

Dell EJ, Connor J, Chen S, Stebbins EG, Skiba NP, Mochly-Rosen D, Hamm HE. The betagamma subunit of heterotrimeric G proteins interacts with RACK1 and two other WD repeat proteins. J. Biol. Chem [print-electronic]. 2002 Dec 12/20/2002; 277(51): 49888-95. PMID: 12359736, PII: M202755200, DOI: 10.1074/jbc.M202755200, ISSN: 0021-9258.

Medkova M, Preininger AM, Yu NJ, Hubbell WL, Hamm HE. Conformational changes in the amino-terminal helix of the G protein alpha(i1) following dissociation from Gbetagamma subunit and activation. Biochemistry. 2002 Aug 8/6/2002; 41(31): 9962-72. PMID: 12146960, PII: bi0255726, ISSN: 0006-2960.

Mukherjee S, Gurevich VV, Preninger A, Hamm HE, Bader MF, Fazleabas AT, Birnbaumer L, Hunzicker-Dunn M. Aspartic acid 564 in the third cytoplasmic loop of the luteinizing hormone/choriogonadotropin receptor is crucial for phosphorylation-independent interaction with arrestin2. J. Biol. Chem [print-electronic]. 2002 May 5/17/2002; 277(20): 17916-27. PMID: 11867621, PII: M110479200, DOI: 10.1074/jbc.M110479200, ISSN: 0021-9258.

Gilchrist A, Li A, Hamm HE. G alpha COOH-terminal minigene vectors dissect heterotrimeric G protein signaling. Sci. STKE. 2002 Feb 2/5/2002; 2002(118): pl1. PMID: 11836477, PII: 2002/118/pl1, DOI: 10.1126/stke.2002.118.pl1, ISSN: 1525-8882.

Schey KL, Busman M, Cook LA, Hamm HE, Hildebrandt JD. Structural characterization of intact G protein gamma subunits by mass spectrometry. Meth. Enzymol. 2002; 344: 586-97. PMID: 11771413, ISSN: 0076-6879.

Gilchrist A, Li A, Hamm HE. Design and use of C-terminal minigene vectors for studying role of heterotrimeric G proteins. Meth. Enzymol. 2002; 344: 58-69. PMID: 11771412, ISSN: 0076-6879.

Dell EJ, Blackmer T, Skiba NP, Daaka Y, Shekter LR, Rosal R, Reuveny E, Hamm HE. Defining G protein beta gamma specificity for effector recognition. Meth. Enzymol. 2002; 344: 421-34. PMID: 11771401, ISSN: 0076-6879.

Cabrera-Vera TM, Thomas TO, Vanhauwe J, Depree KM, Graber SG, Hamm HE. Dissecting receptor-G protein specificity using G alpha chimeras. Meth. Enzymol. 2002; 344: 69-81. PMID: 11771421, ISSN: 0076-6879.

Vanhauwe JF, Thomas TO, Minshall RD, Tiruppathi C, Li A, Gilchrist A, Yoon EJ, Malik AB, Hamm HE. Thrombin Receptors Activate Go Proteins in Endothelial Cells to Regulate Intracellular Calcium and Cell Shape Changes. J Biol Chem. 2002; 277((37)): 34143-9.

Gilchrist A, Vanhauwe JF, Li A, Thomas TO, Voyno-Yasenetskaya T, Hamm HE. G alpha minigenes expressing C-terminal peptides serve as specific inhibitors of thrombin-mediated endothelial activation. J. Biol. Chem [print-electronic]. 2001 Jul 7/13/2001; 276(28): 25672-9. PMID: 11274183, PII: M100914200, DOI: 10.1074/jbc.M100914200, ISSN: 0021-9258.

Blackmer T, Larsen EC, Takahashi M, Martin TF, Alford S, Hamm HE. G protein betagamma subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry. Science. 2001 Apr 4/13/2001; 292(5515): 293-7. PMID: 11303105, PII: 292/5515/293, DOI: 10.1126/science.1058803, ISSN: 0036-8075.

Minadeo N, Layden B, Amari LV, Thomas V, Radloff K, Srinivasan C, Hamm HE, de Freitas DM. Effect of Li+ upon the Mg2+-dependent activation of recombinant Gialpha1. Arch. Biochem. Biophys. 2001 Apr 4/1/2001; 388(1): 7-12. PMID: 11361143, PII: S0003986101922829, ISSN: 0003-9861.

Aris L, Gilchrist A, Rens-Domiano S, Meyer C, Schatz PJ, Dratz EA, Hamm HE. Structural requirements for the stabilization of metarhodopsin II by the C terminus of the alpha subunit of transducin. J. Biol. Chem [print-electronic]. 2001 Jan 1/26/2001; 276(4): 2333-9. PMID: 11018024, PII: M002533200, DOI: 10.1074/jbc.M002533200, ISSN: 0021-9258.

Lopez I, Mak EC, Ding J, Hamm HE, Lomasney JW. A novel bifunctional phospholipase c that is regulated by Galpha 12 and stimulates the Ras/mitogen-activated protein kinase pathway. J. Biol. Chem [print-electronic]. 2001 Jan 1/26/2001; 276(4): 2758-65. PMID: 11022047, PII: M008119200, DOI: 10.1074/jbc.M008119200, ISSN: 0021-9258.

Hamm HE. How activated receptors couple to G proteins. Proc Natl Acad Sci USA. 2001; 98((9)): 4819-21.

Thulin CD, Savage JR, McLaughlin JN, Truscott SM, Old WM, Ahn NG, Resing KA, Hamm HE, Bitensky MW, nd Willardson BM. Modulation of the G Protein Regulator Phosducin by Ca 2+ /Calmodulin-dependent Protein Kinase II Phosphorylation and 14-3-3 Protein Binding. J Biol Chem. 2001; 276((26)): 23805-15.

Thomas TO, Hamm HE. An Intramolecular Contact in Galpha Transducin That Participates in Maintaining Its Intrinsic GDP Release Rate. Mol Cell Biol Res Commun. 2001; 4((5)): 282-91.

Savage JR, McLaughlin JN, Skiba NP, Hamm HE, Willardson BM. Functional roles of the two domains of phosducin and phosducin-like protein. J. Biol. Chem. 2000 Sep 9/29/2000; 275(39): 30399-407. PMID: 10896945, PII: M005120200, DOI: 10.1074/jbc.M005120200, ISSN: 0021-9258.

Mazzoni MR, Taddei S, Giusti L, Rovero P, Galoppini C, D'Ursi A, Albrizio S, Triolo A, Novellino E, Greco G, Lucacchini A, Hamm HE. A galpha(s) carboxyl-terminal peptide prevents G(s) activation by the A(2A) adenosine receptor. Mol. Pharmacol. 2000 Jul; 58(1): 226-36. PMID: 10860945, ISSN: 0026-895X.

Skiba NP, Thomas TO, Hamm HE. G alpha t/G alpha i1 chimeras used to define structural basis of specific functions of G alpha t. Meth. Enzymol. 2000; 315: 502-24. PMID: 10736723, ISSN: 0076-6879.

Yang CS, Skiba NP, Mazzoni MR, Thomas TO, Hamm HE. Fluorescent probes as indicators of conformation changes in transducin on activation. Meth. Enzymol. 2000; 315: 490-502. PMID: 10736722, ISSN: 0076-6879.

Gilchrist A, Li A, Hamm HE. Use of peptides-on-plasmids combinatorial library to identify high-affinity peptides that bind rhodopsin. Meth. Enzymol. 2000; 315: 388-404. PMID: 10736715, ISSN: 0076-6879.

Minshall RD, Tiruppathi C, Vogel SM, Niles WD, Gilchrist A, Hamm HE, Malik AB. Endothelial Cell-surface gp60 Activates Vesicle Formation and Trafficking via Gi-coupled Src Kinase Signaling Pathway. J Cell Biol. 2000; 150((5)): 1057-70.

Gilchrist A, Bünemann M, Li A, Hosey MM, Hamm HE. A dominant-negative strategy for studying roles of G proteins in vivo. J. Biol. Chem. 1999 Mar 3/5/1999; 274(10): 6610-6. PMID: 10037756, ISSN: 0021-9258.

Bae H, Cabrera-Vera TM, Depree KM, Graber SG, Hamm HE. Two Amino Acids within the alpha4 Helix of Galpha(i)(1) mediate Coupling with 5-Hydroxytryptamine 1B Receptors. J Biol Chem. 1999; 274((21)): 14963-71.

Yang CS, Skiba NP, Mazzoni MR, Hamm HE. Conformational Changes at The Carboxyl Terminus of Galpha Occur during G Protein Activation. J Biol Chem. 1999; 274((4)): 2379-85.

Skiba NP, Yang CS, Huang T, Bae H, Hamm HE. The alpha-Helical Domain of Galpha(t) Determines Specific Interaction with Regulator of G Protein Signaling 9. J Biol Chem. 1999; 274((13)): 8770-8.

Ellis CA, Malik AB, Gilchrist A, Hamm HE, Sandoval R, Voyno-Yasenetskaya T, Tiruppathi c. Thrombin Induces Proteinase-activated Receptor-1 Gene Expression in Endothelial Cells via Activation of Gi-linked Ras/ Mitogen-activated Protein Kinase Pathway*. J Biol Chem. 1999; 274((19)): 13718-27.

Gilchrist A, Mazzoni MR, Dineen B, Dice A, Linden J, Proctor WR, Lupica CR, Dunwiddie TV, Hamm HE. Antagonists of the receptor-G protein interface block Gi-coupled signal transduction. J. Biol. Chem. 1998 Jun 6/12/1998; 273(24): 14912-9. PMID: 9614095, ISSN: 0021-9258.

Ford CE, Skiba NP, Bae H, Daaka Y, Reuveny E, Shekter LR, Rosal R, Weng G, Yang CS, Iyengar R, Miller RJ, Jan LY, Lefkowitz RJ, Hamm HE. Molecular basis for interactions of G protein betagamma subunits with effectors. Science. 1998 May 5/22/1998; 280(5367): 1271-4. PMID: 9596582, ISSN: 0036-8075.

Skiba NP, Hamm HE. How Gsalpha activates adenylyl cyclase. Nature structural biology. 1998 Feb; 5(2) Sect. 88,92.

Hamm HE. The many faces of G protein signaling. J. Biol. Chem. 1998 Jan 1/9/1998; 273(2): 669-72. PMID: 9422713, ISSN: 0021-9258.

Bae H, Anderson K, Flood LA, Skiba NP, Hamm HE, Graber SG. Molecular determinants of selectivity in 5-hydroxytryptamine1B receptor-G protein interactions. J. Biol. Chem. 1997 Dec 12/19/1997; 272(51): 32071-7. PMID: 9405403, ISSN: 0021-9258.

Nekrasova ER, Berman DM, Rustandi RR, Hamm HE, Gilman AG, Arshavsky VY. Activation of transducin guanosine triphosphatase by two proteins of the RGS family. Biochemistry. 1997 Jun 6/24/1997; 36(25): 7638-43. PMID: 9201904, PII: bi970427r, DOI: 10.1021/bi970427r, ISSN: 0006-2960.

Mazzoni MR, Hamm HE. Interaction of transducin with light-activated rhodopsin protects It from proteolytic digestion by trypsin. J. Biol. Chem. 1996 Nov 11/22/1996; 271(47): 30034-40. PMID: 8939950, ISSN: 0021-9258.

Artemyev NO, Surendran R, Lee JC, Hamm HE. Subunit structure of rod cGMP-phosphodiesterase. J. Biol. Chem. 1996 Oct 10/11/1996; 271(41): 25382-8. PMID: 8810304, ISSN: 0021-9258.

Hamm HE, Gilchrist A. Heterotrimeric G proteins. Curr. Opin. Cell Biol. 1996 Apr; 8(2): 189-96. PMID: 8791425, PII: S0955-0674(96)80065-2, ISSN: 0955-0674.

Sondek J, Bohm A, Lambright DG, Hamm HE, Sigler PB. Crystal structure of a G-protein beta gamma dimer at 2.1A resolution. Nature. 1996 Jan 1/25/1996; 379(6563): 369-74. PMID: 8552196, DOI: 10.1038/379369a0, ISSN: 0028-0836.

Lambright DG, Sondek J, Bohm A, Skiba NP, Hamm HE, Sigler PB. The 2.0 A crystal structure of a heterotrimeric G protein. Nature. 1996 Jan 1/25/1996; 379(6563): 311-9. PMID: 8552184, DOI: 10.1038/379311a0, ISSN: 0028-0836.

Suh KH, Hamm HE. Cyclic AMP-dependent phosphoprotein components I and II interact with beta gamma subunits of transducin in frog rod outer segments. Biochemistry. 1996 Jan 1/9/1996; 35(1): 290-8. PMID: 8555187, PII: bi9518656, DOI: 10.1021/bi9518656, ISSN: 0006-2960.

Skiba NP, Bae H, Hamm HE. Mapping of effector binding sites of transducin alpha-subunit using G alpha t/G alpha i1 chimeras. J. Biol. Chem. 1996 Jan 1/5/1996; 271(1): 413-24. PMID: 8550597, ISSN: 0021-9258.

Martin EL, Rens-Domiano S, Schatz PJ, Hamm HE. Potent peptide analogues of a G protein receptor-binding region obtained with a combinatorial library. J. Biol. Chem. 1996 Jan 1/5/1996; 271(1): 361-6. PMID: 8550587, ISSN: 0021-9258.

Mazzoni MR, Artemyev NO, Hamm HE. Proteolytic fragmentation for epitope mapping. Methods Mol. Biol. 1996; 66: 109-20. PMID: 8959709, DOI: 10.1385/0-89603-375-9:109, ISSN: 1064-3745.

Artemyev NO, Natochin M, Busman M,Schey KL, Hamm HE. Mechanism of photoreceptor cGMP phosphodiesterase inhibition by its gamma subunits. Biochemistry. 1996; 93((11)): 5407-12.

Rens-Domiano S, Hamm HE. Structural and functional relationships of heterotrimeric G-proteins. FASEB J. 1995 Aug; 9(11): 1059-66. PMID: 7649405, ISSN: 0892-6638.

Skiba NP, Artemyev NO, Hamm HE. The carboxyl terminus of the gamma-subunit of rod cGMP phosphodiesterase contains distinct sites of interaction with the enzyme catalytic subunits and the alpha-subunit of transducin. J. Biol. Chem. 1995 Jun 6/2/1995; 270(22): 13210-5. PMID: 7768919, ISSN: 0021-9258.

Slepak, V Z, Artemyev, N O, Zhu, Y, Dumke, C L, Sabacan, L, Sondek, J, Hamm, H E, Bownds, M D, and Arshavsky, V Y. An Effector Site That Stimulates G-protein GTPase in Photoreceptors. J Biol Chem. 1995; 270((24)): 14319-24.

Sondek J, Lambright DG, Noel JP, Hamm HE, Sigler PB. GTPase mechanism of Gproteins from the 1.7-A crystal structure of transducin alpha-GDP-AIF-4. Nature. 1994 Nov 11/17/1994; 372(6503): 276-9. PMID: 7969474, DOI: 10.1038/372276a0, ISSN: 0028-0836.

Artemyev NO, Hamm HE. Probing G-protein function. Nature structural biology. 1994 Nov; 1(11) Sect. 752,4.

Rasenick MM, Watanabe M, Lazarevic MB, Hatta S, Hamm HE. Synthetic peptides as probes for G protein function. Carboxyl-terminal G alpha s peptides mimic Gs and evoke high affinity agonist binding to beta-adrenergic receptors. J. Biol. Chem. 1994 Aug 8/26/1994; 269(34): 21519-25. PMID: 8063788, ISSN: 0021-9258.

Arshavsky VY, Dumke CL, Zhu Y, Artemyev NO, Skiba NP, Hamm HE, Bownds MD. Regulation of transducin GTPase activity in bovine rod outer segments. J. Biol. Chem. 1994 Aug 8/5/1994; 269(31): 19882-7. PMID: 8051070, ISSN: 0021-9258.

Lambright DG, Noel JP, Hamm HE, Sigler PB. Structural determinants for activation of the alpha-subunit of a heterotrimeric G protein. Nature. 1994 Jun 6/23/1994; 369(6482): 621-8. PMID: 8208289, DOI: 10.1038/369621a0, ISSN: 0028-0836.

Krupnick JG, Gurevich VV, Schepers T, Hamm HE, Benovic JL. Arrestin-rhodopsin interaction. Multi-site binding delineated by peptide inhibition. J. Biol. Chem. 1994 Feb 2/4/1994; 269(5): 3226-32. PMID: 8106358, ISSN: 0021-9258.

Hamm HE, Rarick HM. Specific peptide probes for G-protein interactions with receptors. Meth. Enzymol. 1994; 237: 423-36. PMID: 7935015, PII: S0076-6879(94)37079-6, ISSN: 0076-6879.

Rarick HM, Artemyev NO, Mills JS, Skiba NP, Hamm HE. Specific peptide probes for G-protein interaction with effectors. Meth. Enzymol. 1994; 238: 13-28. PMID: 7799779, ISSN: 0076-6879.

Noel JP, Hamm HE, Sigler PB. The 2.2 A crystal structure of transducin-alpha complexed with GTP gamma S. Nature. 1993 Dec 12/16/1993; 366(6456): 654-63. PMID: 8259210, DOI: 10.1038/366654a0, ISSN: 0028-0836.

Artemyev NO, Mills JS, Thornburg KR, Knapp DR, Schey KL, Hamm HE. A site on transducin alpha-subunit of interaction with the polycationic region of cGMP phosphodiesterase inhibitory subunit. J. Biol. Chem. 1993 Nov 11/5/1993; 268(31): 23611-5. PMID: 8226888, ISSN: 0021-9258.

Mazzoni MR, Hamm HE. Tryptophan207 is involved in the GTP-dependent conformational switch in the alpha subunit of the G protein transducin: chymotryptic digestion patterns of the GTP gamma S and GDP-bound forms. J. Protein Chem. 1993 Apr; 12(2): 215-21. PMID: 8489707, ISSN: 0277-8033.

Hargrave PA, Hamm HE, Hofmann KP. Interaction of rhodopsin with the G-protein, transducin. Bioessays. 1993 Jan; 15(1): 43-50. PMID: 8466475, DOI: 10.1002/bies.950150107, ISSN: 0265-9247.

Dratz, EA, Furstenau, JE, Lambert, CG, Thireault, DL, Rarick, HR, Schepers, T, Pakhlevaniants, S., and H.E. Hamm. NMR structure of a receptor bound G protein peptide. Nature. 1993; 363((6426)): 276-81.

Artemyev NO, Rarick HM, Mills JS, Skiba NP, Hamm HE. Sites of interaction between rod G-protein alpha-subunit and cGMP-phosphodiesterase gamma-subunit. Implications for the phosphodiesterase activation mechanism. J. Biol. Chem. 1992 Dec 12/15/1992; 267(35): 25067-72. PMID: 1334079, ISSN: 0021-9258.

Stieve H, Niemeyer B, Aktories K, Hamm HE. Disturbing GTP-binding protein function through microinjection into the visual cell of Limulus. Z. Naturforsch., C, J. Biosci. 1992 Nov; 47(11-12): 915-21. PMID: 1285831, ISSN: 0939-5075.

Artemyev, N O, Hamm, H E. Two-site high-affinity interaction between inhibitory and catalytic subunits of rod cyclic GMP phosphodiesterase. Biochem J. 1992; 283((Pt 1)): 273-9.

Mangels, L A, Neubig, R R, Hamm, H E, Gnegy, M E. Calmodulin binding distinguishes between betagamma subunits of activated G proteins and transducin. Biochem J. 1992; 283((Pt 3)): 683-90.

Rarick HM, Artemyev NO, Hamm HE. A site on rod G protein alpha subunit that mediates effector activation. Science. 1992; 256((5059)): 1031-3.

Hamm HE. Molecular interactions between the photoreceptor G protein and rhodopsin. Cell. Mol. Neurobiol. 1991 Dec; 11(6): 563-78. PMID: 1782650, ISSN: 0272-4340.

Mazzoni MR, Malinski JA, Hamm HE. Structural analysis of rod GTP-binding protein, Gt. Limited proteolytic digestion pattern of Gt with four proteases defines monoclonal antibody epitope. J. Biol. Chem. 1991 Jul 7/25/1991; 266(21): 14072-81. PMID: 1713215, ISSN: 0021-9258.

Warpeha, K M, Hamm, H E, Rasenick, M M, Kaufman, L S. A blue-light-activated GTP-binding protein in the plasma membranes of etiolated peas. Proc Natl Acad Sci USA. 1991; 88: 8925-9.

Hamm HE, Rarick H, Mazzoni M, Malinski J, Suh KH. The molecular basis of GTP-binding protein interaction with receptors. Biochem. Soc. Symp. 1990; 56: 35-44. PMID: 2256961, ISSN: 0067-8694.

Hamm HE. Surfaces of interaction between Gt and rhodopsin in the GDP-bound and empty-pocket configurations. Adv. Second Messenger Phosphoprotein Res. 1990; 24: 76-82. PMID: 2119661, ISSN: 1040-7952.

Robinson, P R, Wood, S F, Szuts, E Z, Fein, A, Hamm, H E, Lisman, J E. Light-dependent GTP-binding proteins in squid photoreceptors. Biochem j. 1990; 272((1)): 79-85.

Mazzoni MR, Hamm HE. Effect of monoclonal antibody binding on alpha-beta gamma subunit interactions in the rod outer segment G protein, Gt. Biochemistry. 1989 Dec 12/12/1989; 28(25): 9873-80. PMID: 2482080, ISSN: 0006-2960.

Hamm HE, Deretic D, Mazzoni MR, Moore CA, Takahashi JS, Rasenick MM. A monoclonal antibody against the rod outer segment guanyl nucleotide-binding protein, transducin, blocks the stimulatory and inhibitory G proteins of adenylate cyclase. J. Biol. Chem. 1989 Jul 7/5/1989; 264(19): 11475-82. PMID: 2544596, ISSN: 0021-9258.

Pepe IM, Panfoli I, Hamm HE. Visual transduction in vertebrate photoreceptors. Light activation of guanylate cyclase. Cell Biophys. 1989 Apr; 14(2): 129-37. PMID: 2472205, DOI: 10.1007/BF02797130, ISSN: 0163-4992.

Hamm HE, Deretic D, Arendt A, Hargrave PA, Koenig B, Hofmann KP. Site of G protein binding to rhodopsin mapped with synthetic peptides from the alpha subunit. Science. 1988 Aug 8/12/1988; 241(4867): 832-5. PMID: 3136547, ISSN: 0036-8075.

Yatani A, Hamm H, Codina J, Mazzoni MR, Birnbaumer L, Brown AM. A monoclonal antibody to the alpha subunit of Gk blocks muscarinic activation of atrial K+ channels. Science. 1988 Aug 8/12/1988; 241(4867): 828-31. PMID: 2457252, ISSN: 0036-8075.

Deretic D, Hamm HE. Topographic analysis of antigenic determinants recognized by monoclonal antibodies to the photoreceptor guanyl nucleotide-binding protein, transducin. J. Biol. Chem. 1987 Aug 8/5/1987; 262(22): 10839-47. PMID: 2440876, ISSN: 0021-9258.

Hamm HE, Deretic D, Hofmann KP, Schleicher A, Kohl B. Mechanism of action of monoclonal antibodies that block the light activation of the guanyl nucleotide-binding protein, transducin. J. Biol. Chem. 1987 Aug 8/5/1987; 262(22): 10831-8. PMID: 2440875, ISSN: 0021-9258.

Hamm HE, Bownds MD. Protein complement of rod outer segments of frog retina. Biochemistry. 1986 Aug 8/12/1986; 25(16): 4512-23. PMID: 3021191, ISSN: 0006-2960.

Hamm, H E, Bownds, M D. A monoclonal antibody to guanine nucleotide binding protein inhibits the light-activated cyclic GMP pathway in frog rod outer segments. J Gen Physiol. 1984; 84((2)): 265-80.

Witt, P L, Hamm, H E, Bownds, M D. Preparation and characterization of monoclonal antibodies to several frog rod outer segment proteins. J Gen Physiol. 1984; 84((2)): 251-63.

Hamm HE, Takahashi JS, Menaker M. Light-induced decrease of serotonin N-acetyltransferase activity and melatonin in the chicken pineal gland and retina. Brain Res. 1983 May 5/5/1983; 266(2): 287-93. PMID: 6871664, PII: 0006-8993(83)90660-1, ISSN: 0006-8993.

Hermolin, J, Karell, M A, Hamm, H E, Bownds, M D. Calcium and cyclic GMP regulation of light-sensitive protein phosphorylation in frog photoreceptor membranes. J Gen Physiol. 1982; 79((4)): 633-55.

Hamm HE, Menaker M. Pineal and retinal serotonin N-acetyltransferase activity: modulation by phosphate. J. Neurochem. 1981 Dec; 37(6): 1567-72. PMID: 7334376, ISSN: 0022-3042.

Hamm, H E, Menaker, M. Retinal rhythms in chicks: circadian variation in melantonin and serotonin N-acetyltransferase activity. Proc Natl Acad Sci USA. 1980; 77((8)): 4998-5002.

Available Postdoctoral Position Details
Posted Position

Seeking postdoctoral fellows to study PAR4 signaling in platelets. We are interested in PAR4 as a target for treatment of inflammatory, cardiovascular and cerebrovascular diseases. We are also developing antagonists of human PAR4 as well as mouse PAR4 for animal studies. JOB REQUIREMENTS: Applicant should have a recent PhD in cell biology, pharmacology, biochemistry, molecular biology or neuroscience; with good background in one or more of the following: (1) training in pharmacology 2) translational studies in platelet biochemistry or signaling 3) training in inflammation APPLICATION REQUIREMENTS: Send CV, bibliography, and a brief statement of your background and research interests to Dr. Heidi Hamm. Please send the names and addresses of three references with the application. APPLICATION DEADLINE: June 1, 2019, but could start earlier. Please submit your CV with contact information for 3 references to Dr. Heidi Hamm


A postdoctoral position is available in the laboratory of Heidi Hamm, Ph.D,. We are looking for an individual whose research interests include exocytosis and receptor signaling. The project entails characterization of the effect of Gi/o-coupled GPCR signaling on the release of neurotransmitters and hormones from primary cells using genetic and pharmacological manipulation. A background in molecular biology and cellular neuroscience is desirable. Preference will be given to applicants with experience in the production and utilization of viral transduction systems, including lentivirus, adenovirus, or adeno-associated virus. Qualified applicants must possess a doctoral degree with a strong background in biomedical research, including publications in peer-reviewed academic journals. Candidates should submit a curriculum vitae and three letters of reference to heidi.hamm@vanderbilt.edu.