Faculty Appointments
Professor of Medicine
Professor of Cell & Developmental BiologyRuth King Scoville Chair in MedicineProfessor of Pharmacology
Education
M.D., Medicine, Vanderbilt University, Nashville, TennesseePh.D., Pharmacology, Vanderbilt University, Nashville, TennesseeB.S., Chemistry, Georgetown College, Georgetown, Kentucky
Office Address
S-3223 MCN
TN 37221
TN 37221
Research Description
Gene transfer to the kidney remains understudied
This is unfortunate as chronic kidney disease plagues up to 14% of the U.S. population. Dialysis remains the mainline treatment for end stage renal disease (ESRD) as kidney transplants are very limited given organ availability. The mortality of patients on dialysis (5 year survival ~20% for patients with ESRD and diabetes) is equivalent if not worse than many cancers which receive quite a bit of support for cell and gene therapy approaches.
Gene transfer approaches that could cure or limit progression of kidney disease would have a high impact on eliminating the widespread mortality and morbidity associated with kidney disease.
We use a combination of approaches to attack kidney disease including:
Nonviral (transposons)
Viral (adeno-associated viruses)
Genome editing (CRISPR/Cas9)
New technologies
Animal models of inherited kidney diseases
lab website: https://medsites.vumc.org/mattwilsonlab
This is unfortunate as chronic kidney disease plagues up to 14% of the U.S. population. Dialysis remains the mainline treatment for end stage renal disease (ESRD) as kidney transplants are very limited given organ availability. The mortality of patients on dialysis (5 year survival ~20% for patients with ESRD and diabetes) is equivalent if not worse than many cancers which receive quite a bit of support for cell and gene therapy approaches.
Gene transfer approaches that could cure or limit progression of kidney disease would have a high impact on eliminating the widespread mortality and morbidity associated with kidney disease.
We use a combination of approaches to attack kidney disease including:
Nonviral (transposons)
Viral (adeno-associated viruses)
Genome editing (CRISPR/Cas9)
New technologies
Animal models of inherited kidney diseases
lab website: https://medsites.vumc.org/mattwilsonlab
Research Keywords
We use cutting-edge technologies to develop new therapies for kidney disease.
Publications
Qian ES, Bejoy J, Welch RC, Farry JM, Hartert J, Cartailler JP, Gibson-Corley KN, Wilson MH, Woodard LE. Urine-derived stem cell extracellular vesicles promote recovery from acute kidney injury in mice and human kidney organoids. Am J Physiol Renal Physiol [print-electronic]. 2026 Aug 8/1/2026; 331(2): F100-F117-F117. PMID: 42222936, DOI: 10.1152/ajprenal.00304.2025, ISSN: 1522-1466.
Carty JS, Selvasingh J, Zuchowski Y, Nam HJ, Pénalva C, Nanayakkara G, Jennings EQ, Voss K, Adame ET, Tossberg JT, Yap WS, Melzer M, Viquez O, McCall AS, Piotrowski ER, Bessho R, Cao S, Leaptrot KL, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, Trapani JB, Cottam MA, Wan M, Shrivastava D, Delker DA, Wilson MH, Hasenour CM, Lantier L, Chernova I, Young JD, Haase VH, Vazquez-Medina JP, Kosma DK, Kim PK, Cartailler JP, Zhang M, Zent R, Harris RC, Watts JA, Terker AS, Bock F, Rathmell JC, Rodan AR, Arroyo JP. Dehydration promotes intracellular lipid synthesis and accumulation. Nat Commun. 2026 May 5/25/2026; 17(1): PMID: 42185304, PMCID: PMC13385796, PII: 10.1038/s41467-026-73534-x, DOI: 10.1038/s41467-026-73534-x, ISSN: 2041-1723.
Hickman PG, Asokan A, Wilson MH. Rewriting Renal Fate: The Evolving Landscape of Adeno-Associated Virus-Mediated Kidney Gene Therapies. Kidney360 [print-electronic]. 2026 Feb 2/1/2026; 7(2): 422-5. PMID: 41442198, PMCID: PMC12935359, PII: 02200512-202602000-00027, DOI: 10.34067/KID.0000001119, ISSN: 2641-7650.
Peek JL, Rosales A, Qi J, Feleke L, Menshikh A, Welch RC, Woodard LE, Asokan A, Wilson MH. Efficient kidney gene transfer and proximal tubule transduction using self-complementary AAV.cc47 vectors. Mol Ther Methods Clin Dev. 2025 Dec 12/11/2025; 33(4): 101606. PMID: 41146875, PMCID: PMC12554119, PII: S2329-0501(25)00201-3, DOI: 10.1016/j.omtm.2025.101606, ISSN: 2329-0501.
M Viquez O, Melzer M, Li S, Tantengco M, Dong X, Sha E, Huang J, Krystofiak ES, Hart RC, Luo W, Warren C, Bayazid AB, Zhang R, Bessho R, Haase VH, Brakebusch C, Inoue T, Brooks C, Wilson MH, Terker AS, Arroyo JP, Pozzi A, Zent R, Bock F. Rac1 promotes proximal tubule kidney repair by coupling the actin cytoskeleton to mitochondrial function. Proc Natl Acad Sci U S A [print-electronic]. 2025 Nov 11/25/2025; 122(47): e2504565122. PMID: 41248288, PMCID: PMC12626402, DOI: 10.1073/pnas.2504565122, ISSN: 1091-6490.
Woodard LE, Welch RC, Menshikh A, Luo W, Williams FM, Peek JL, Sha F, Veach RA, Kaja A, Beckermann TM, Ikizler TA, Wilson MH. Partial correction of cystinuria type A in mice via kidney-targeted transposon delivery. Mol Ther Nucleic Acids. 2025 Sep 9/9/2025; 36(3): 102639. PMID: 40791872, PMCID: PMC12337122, PII: S2162-2531(25)00193-3, DOI: 10.1016/j.omtn.2025.102639, ISSN: 2162-2531.
Bejoy J, Welch RC, Qian ES, Williams FM, Gibson-Corley KN, Wilson MH, Paragas N, Woodard LE. Urine-derived stem cells display homing, incorporation, and regeneration in human organoid and mouse models of acute kidney injury. Mol Ther [print-electronic]. 2025 Jul 7/2/2025; 33(7): 3307-20. PMID: 40158205, PMCID: PMC12266040, PII: S1525-0016(25)00219-9, DOI: 10.1016/j.ymthe.2025.03.044, ISSN: 1525-0024.
Rosales A, Blondel LO, Hull J, Gao Q, Aykun N, Peek JL, Vargas A, Fergione S, Song M, Wilson MH, Barbas AS, Asokan A. Evolving adeno-associated viruses for gene transfer to the kidney via cross-species cycling of capsid libraries. Nat Biomed Eng [print-electronic]. 2025 Jul; 9(7): 1086-100. PMID: 39910375, PMCID: PMC12261118, PII: 10.1038/s41551-024-01341-0, DOI: 10.1038/s41551-024-01341-0, ISSN: 2157-846X.
Asokan A, Wilson MH. Filtering through AAV capsid libraries for effective kidney gene transfer. Kidney Int [print-electronic]. 2025 Apr; 107(4): 591-3. PMID: 39814263, PII: S0085-2538(25)00014-6, DOI: 10.1016/j.kint.2024.12.012, ISSN: 1523-1755.
Hickman AB, Lannes L, Furman CM, Hong C, Franklin L, Ghirlando R, Ghosh A, Luo W, Konstantinidou P, Lorenzi HA, Grove A, Haase AD, Wilson MH, Dyda F. Activity of the mammalian DNA transposon piggyBat from Myotis lucifugus is restricted by its own transposon ends. Nat Commun. 2025 Jan 1/7/2025; 16(1): 458. PMID: 39774116, PMCID: PMC11707139, PII: 10.1038/s41467-024-55784-9, DOI: 10.1038/s41467-024-55784-9, ISSN: 2041-1723.
Cao S, Pan Y, Terker AS, Arroyo Ornelas JP, Wang Y, Tang J, Niu A, Kar SA, Jiang M, Luo W, Dong X, Fan X, Wang S, Wilson MH, Fogo A, Zhang MZ, Harris RC. Epidermal growth factor receptor activation is essential for kidney fibrosis development. Nat Commun. 2023 Nov 11/14/2023; 14(1): 7357. PMID: 37963889, PMCID: PMC10645887, PII: 10.1038/s41467-023-43226-x, DOI: 10.1038/s41467-023-43226-x, ISSN: 2041-1723.
Peek JL, Wilson MH. Cell and gene therapy for kidney disease. Nat Rev Nephrol [print-electronic]. 2023 Jul; 19(7): 451-62. PMID: 36973494, PMCID: PMC10339687, PII: 10.1038/s41581-023-00702-3, DOI: 10.1038/s41581-023-00702-3, ISSN: 1759-507X.
Kenigsberg Z, Welch RC, Bejoy J, Williams FM, Veach RA, Jarrett I, Thompson TK, Wilson MH, Woodard LE. Genome Engineering of Human Urine-Derived Stem Cells to Express Lactoferrin and Deoxyribonuclease. Tissue Eng Part A [print-electronic]. 2023 Jul; 29(13-14): 372-83. PMID: 37130035, PMCID: PMC10354709, DOI: 10.1089/ten.TEA.2023.0003, ISSN: 1937-335X.
Pan Y, Cao S, Terker AS, Tang J, Sasaki K, Wang Y, Niu A, Luo W, Daassi D, Fan X, Wang S, Wilson MH, Zhang MZ, Harris RC. Corrigendum to "Myeloid cyclooxygenase-2/prostaglandin E2/E-type prostanoid receptor 4 promotes transcription factor MafB-dependent inflammatory resolution in acute kidney injury." Kidney Int. 2022;101:79-91. Kidney Int. 2023 Apr; 103(4): 798. PMID: 36948771, PII: S0085-2538(23)00062-5, DOI: 10.1016/j.kint.2023.01.014, ISSN: 1523-1755.
Hunter K, Larsen JA, Love HD, Evans RC, Roy S, Zent R, Harris RC, Wilson MH, Fissell WH. Inhibition of Transforming Growth Factor-ß Improves Primary Renal Tubule Cell Differentiation in Long-Term Culture. Tissue Eng Part A [print-electronic]. 2023 Feb; 29(3-4): 102-11. PMID: 36274231, PMCID: PMC10081716, DOI: 10.1089/ten.TEA.2022.0147, ISSN: 1937-335X.
Pan Y, Cao S, Terker AS, Tang J, Sasaki K, Wang Y, Niu A, Luo W, Daasi D, Fan X, Wang S, Wilson MH, Zhang MZ, Harris RC. Corrigendum to "Myeloid cyclooxygenase-2/prostaglandin E2/E-type prostanoid receptor 4 promotes transcription factor MafB-dependent inflammatory resolution in acute kidney injury." Kidney Int. 2022;101:79-91. Kidney Int. 2023 Jan; 103(1): 229. PMID: 36603974, PMCID: PMC10003615, PII: S0085-2538(22)00909-7, DOI: 10.1016/j.kint.2022.10.005, ISSN: 1523-1755.
Arroyo JP, Terker AS, Zuchowski Y, Watts JA, Bock F, Meyer C, Luo W, Kapp ME, Gould ER, Miranda AX, Carty J, Jiang M, Vanacore RM, Hammock E, Wilson MH, Zent R, Zhang M, Bhave G, Harris RC. Kidney collecting duct cells make vasopressin in response to NaCl-induced hypertonicity. JCI Insight. 2022 Dec 12/22/2022; 7(24): PMID: 36326835, PMCID: PMC9869977, PII: 161765, DOI: 10.1172/jci.insight.161765, ISSN: 2379-3708.
Luo W, Hickman AB, Genzor P, Ghirlando R, Furman CM, Menshikh A, Haase A, Dyda F, Wilson MH. Transposase N-terminal phosphorylation and asymmetric transposon ends inhibit piggyBac transposition in mammalian cells. Nucleic Acids Res. 2022 Dec 12/9/2022; 50(22): 13128-42. PMID: 36537219, PMCID: PMC9825180, PII: 6946998, DOI: 10.1093/nar/gkac1191, ISSN: 1362-4962.
Haake SM, Plosa EJ, Kropski JA, Venton LA, Reddy A, Bock F, Chang BT, Luna AJ, Nabukhotna K, Xu ZQ, Prather RA, Lee S, Tanjore H, Polosukhin VV, Viquez OM, Jones A, Luo W, Wilson MH, Rathmell WK, Massion PP, Pozzi A, Blackwell TS, Zent R. Ligand-independent integrin ß1 signaling supports lung adenocarcinoma development. JCI Insight. 2022 Aug 8/8/2022; 7(15): PMID: 35763345, PMCID: PMC9462485, PII: 154098, DOI: 10.1172/jci.insight.154098, ISSN: 2379-3708.
Peek JL, Wilson MH. Gene therapy for kidney disease: targeting cystinuria. Curr Opin Nephrol Hypertens. 2022 Mar 3/1/2022; 31(2): 175-9. PMID: 34982522, PMCID: PMC8799525, PII: 00041552-202203000-00008, DOI: 10.1097/MNH.0000000000000768, ISSN: 1473-6543.
Pan Y, Cao S, Terker AS, Tang J, Sasaki K, Wang Y, Niu A, Luo W, Fan X, Wang S, Wilson MH, Zhang MZ, Harris RC. Myeloid cyclooxygenase-2/prostaglandin E2/E-type prostanoid receptor 4 promotes transcription factor MafB-dependent inflammatory resolution in acute kidney injury. Kidney Int [print-electronic]. 2022 Jan; 101(1): 79-91. PMID: 34774558, PMCID: PMC8741730, PII: S0085-2538(21)01032-2, DOI: 10.1016/j.kint.2021.09.033, ISSN: 1523-1755.
Wilson MH, Gottschalk S. Expect the unexpected: piggyBac and lymphoma [editorial]. Blood. 2021 Oct 10/21/2021; 138(16): 1379-80. PMID: 34673949, PII: S0006-4971(21)01729-8, DOI: 10.1182/blood.2021012349, ISSN: 1528-0020.
Beckermann TM, Luo W, Wilson CM, Veach RA, Wilson MH. Cognate restriction of transposition by piggyBac-like proteins. Nucleic Acids Res. 2021 Aug 8/20/2021; 49(14): 8135-44. PMID: 34232995, PMCID: PMC8373079, PII: 6316838, DOI: 10.1093/nar/gkab578, ISSN: 1362-4962.
Reinfeld BI, Madden MZ, Wolf MM, Chytil A, Bader JE, Patterson AR, Sugiura A, Cohen AS, Ali A, Do BT, Muir A, Lewis CA, Hongo RA, Young KL, Brown RE, Todd VM, Huffstater T, Abraham A, O'Neil RT, Wilson MH, Xin F, Tantawy MN, Merryman WD, Johnson RW, Williams CS, Mason EF, Mason FM, Beckermann KE, Vander Heiden MG, Manning HC, Rathmell JC, Rathmell WK. Cell-programmed nutrient partitioning in the tumour microenvironment. Nature [print-electronic]. 2021 May; 593(7858): 282-8. PMID: 33828302, PMCID: PMC8122068, PII: 10.1038/s41586-021-03442-1, DOI: 10.1038/s41586-021-03442-1, ISSN: 1476-4687.
Woodard LE, Williams FM, Jarrett IC, Wilson MH. Functional analysis of the catalytic triad of the hAT-family transposase TcBuster. Plasmid [print-electronic]. 2021 Mar; 114: 102554. PMID: 33476638, PMCID: PMC7946762, PII: S0147-619X(21)00001-9, DOI: 10.1016/j.plasmid.2021.102554, ISSN: 1095-9890.
Love H, Evans R, Humes HD, Roy S, Zent R, Harris R, Wilson M, Fissell WH. Metformin and Inhibition of Transforming Growth Factor-Beta Stimulate In Vitro Transport in Primary Renal Tubule Cells. Tissue Eng Part A. 2020 Oct; 26(19-20): 1091-8. PMID: 32312181, PMCID: PMC7580646, DOI: 10.1089/ten.TEA.2019.0294, ISSN: 1937-335X.
Chiusa M, Hu W, Zienkiewicz J, Chen X, Zhang MZ, Harris RC, Vanacore RM, Bentz JA, Remuzzi G, Benigni A, Fogo AB, Luo W, Mili S, Wilson MH, Zent R, Hawiger J, Pozzi A. EGF receptor-mediated FUS phosphorylation promotes its nuclear translocation and fibrotic signaling. J Cell Biol. 2020 Sep 9/7/2020; 219(9): PMID: 32678881, PMCID: PMC7480104, PII: 151955, DOI: 10.1083/jcb.202001120, ISSN: 1540-8140.
Chen Q, Luo W, Veach RA, Hickman AB, Wilson MH, Dyda F. Structural basis of seamless excision and specific targeting by piggyBac transposase. Nat Commun. 2020 Jul 7/10/2020; 11(1): 3446. PMID: 32651359, PMCID: PMC7351741, PII: 10.1038/s41467-020-17128-1, DOI: 10.1038/s41467-020-17128-1, ISSN: 2041-1723.
Beckermann TM, Welch RC, Williams FM, Mortlock DP, Sha F, Ikizler TA, Woodard LE, Wilson MH. CRISPR/Cas9 engineering of albino cystinuria Type A mice. Genesis [print-electronic]. 2020 May; 58(5): e23357. PMID: 32078250, DOI: 10.1002/dvg.23357, ISSN: 1526-968X.
Wilson MH, Veach RA, Luo W, Welch RC, Roy S, Fissell WH. Genome Engineering Renal Epithelial Cells for Enhanced Volume Transport Function. Cell Mol Bioeng. 2020 Feb; 13(1): 17-26. PMID: 32030105, PMCID: PMC6981331, PII: 601, DOI: 10.1007/s12195-019-00601-3, ISSN: 1865-5025.
Woodard LE, Welch RC, Veach RA, Beckermann TM, Sha F, Weinman EJ, Ikizler TA, Tischfield JA, Sahota A, Wilson MH. Metabolic consequences of cystinuria. BMC Nephrol. 2019 Jun 6/20/2019; 20(1): 227. PMID: 31221135, PMCID: PMC6585015, PII: 10.1186/s12882-019-1417-8, DOI: 10.1186/s12882-019-1417-8, ISSN: 1471-2369.
Vanslambrouck JM, Woodard LE, Suhaimi N, Williams FM, Howden SE, Wilson SB, Lonsdale A, Er PX, Li J, Maksimovic J, Oshlack A, Wilson MH, Little MH. Direct reprogramming to human nephron progenitor-like cells using inducible piggyBac transposon expression of SNAI2-EYA1-SIX1. Kidney Int [print-electronic]. 2019 May; 95(5): 1153-66. PMID: 30827514, PMCID: PMC6478531, PII: S0085-2538(19)30038-9, DOI: 10.1016/j.kint.2018.11.041, ISSN: 1523-1755.
Wilson MH. Consider Changing the Horse for Your CAR-T?. Mol. Ther [print-electronic]. 2018 Aug 8/1/2018; 26(8): 1873-4. PMID: 30078438, PMCID: PMC6094485, PII: S1525-0016(18)30277-6, DOI: 10.1016/j.ymthe.2018.06.018, ISSN: 1525-0024.
Hamada M, Nishio N, Okuno Y, Suzuki S, Kawashima N, Muramatsu H, Tsubota S, Wilson MH, Morita D, Kataoka S, Ichikawa D, Murakami N, Taniguchi R, Suzuki K, Kojima D, Sekiya Y, Nishikawa E, Narita A, Hama A, Kojima S, Nakazawa Y, Takahashi Y. Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR. EBioMedicine [print-electronic]. 2018 Aug; 34: 18-26. PMID: 30082227, PMCID: PMC6116345, PII: S2352-3964(18)30251-2, DOI: 10.1016/j.ebiom.2018.07.008, ISSN: 2352-3964.
O'Neil RT, Saha S, Veach RA, Welch RC, Woodard LE, Rooney CM, Wilson MH. Transposon-modified antigen-specific T lymphocytes for sustained therapeutic protein delivery in vivo. Nat Commun. 2018 Apr 4/10/2018; 9(1): 1325. PMID: 29636469, PMCID: PMC5893599, PII: 10.1038/s41467-018-03787-8, DOI: 10.1038/s41467-018-03787-8, ISSN: 2041-1723.
Morita D, Nishio N, Saito S, Tanaka M, Kawashima N, Okuno Y, Suzuki S, Matsuda K, Maeda Y, Wilson MH, Dotti G, Rooney CM, Takahashi Y, Nakazawa Y. Enhanced Expression of Anti-CD19 Chimeric Antigen Receptor in piggyBac Transposon-Engineered T Cells. Mol Ther Methods Clin Dev. 2018 Mar 3/16/2018; 8: 131-40. PMID: 29687032, PMCID: PMC5907825, PII: S2329-0501(17)30128-6, DOI: 10.1016/j.omtm.2017.12.003, ISSN: 2329-0501.
Woodard LE, Welch RC, Williams FM, Luo W, Cheng J, Wilson MH. Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney. J Vis Exp. 2018 Jan 1/8/2018; (131): PMID: 29364221, PMCID: PMC5907682, DOI: 10.3791/56324, ISSN: 1940-087X.
Veach RA, Wilson MH. CRISPR/Cas9 engineering of a KIM-1 reporter human proximal tubule cell line. PLoS ONE. 2018; 13(9): e0204487. PMID: 30260998, PMCID: PMC6160069, PII: PONE-D-17-41126, DOI: 10.1371/journal.pone.0204487, ISSN: 1932-6203.
Luo W, Galvan DL, Woodard LE, Dorset D, Levy S, Wilson MH. Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells. Nucleic Acids Res. 2017 Aug 8/21/2017; 45(14): 8411-22. PMID: 28666380, PII: 3897173, DOI: 10.1093/nar/gkx572, ISSN: 1362-4962.
Woodard LE, Cheng J, Welch RC, Williams FM, Luo W, Gewin LS, Wilson MH. Kidney-specific transposon-mediated gene transfer in vivo. Sci Rep. 2017 Mar 3/20/2017; 7: 44904. PMID: 28317878, PMCID: PMC5357952, PII: srep44904, DOI: 10.1038/srep44904, ISSN: 2045-2322.
Woodard LE, Downes LM, Lee YC, Kaja A, Terefe ES, Wilson MH. Temporal self-regulation of transposition through host-independent transposase rodlet formation. Nucleic Acids Res [print-electronic]. 2017 Jan 1/9/2017; 45(1): 353-66. PMID: 27899587, PMCID: PMC5224482, PII: gkw1115, DOI: 10.1093/nar/gkw1115, ISSN: 1362-4962.
Held EP, Iglesia EA, Johnson AS, Fang JY, Wilson MH, Abel TW, Lake W, Siew ED. Neurosarcoidosis Presenting as Aseptic Meningitis in an Immunosuppressed Renal Transplant Recipient. Transplantation. 2016 Oct; 100(10): e96-e100. PMID: 26863472, DOI: 10.1097/TP.0000000000001074, ISSN: 1534-6080.
Nakazawa Y, Matsuda K, Kurata T, Sueki A, Tanaka M, Sakashita K, Imai C, Wilson MH, Koike K. Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34(+) cells of juvenile myelomonocytic leukemia. J Hematol Oncol. 2016 Mar 3/16/2016; 9: 27. PMID: 26983639, PMCID: PMC4793548, PII: 10.1186/s13045-016-0256-3, DOI: 10.1186/s13045-016-0256-3, ISSN: 1756-8722.
Woodard LE, Wilson MH. PiggyBac-ing models and new therapeutic strategies. Trends Biotechnol [print-electronic]. 2015 Sep; 33(9): 525-33. PMID: 26211958, PMCID: PMC4663986, PII: S0167-7799(15)00137-7, DOI: 10.1016/j.tibtech.2015.06.009, ISSN: 1879-3096.
Liang M, Woodard LE, Liang A, Luo J, Wilson MH, Mitch WE, Cheng J. Protective role of insulin-like growth factor-1 receptor in endothelial cells against unilateral ureteral obstruction-induced renal fibrosis. Am. J. Pathol [print-electronic]. 2015 May; 185(5): 1234-50. PMID: 25783760, PMCID: PMC4419212, PII: S0002-9440(15)00126-1, DOI: 10.1016/j.ajpath.2015.01.027, ISSN: 1525-2191.
Saha S, Woodard LE, Charron EM, Welch RC, Rooney CM, Wilson MH. Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells. Nucleic Acids Res [print-electronic]. 2015 Feb 2/18/2015; 43(3): 1770-82. PMID: 25605795, PMCID: PMC4330379, PII: gkv017, DOI: 10.1093/nar/gkv017, ISSN: 1362-4962.
Galvan DL, O'Neil RT, Foster AE, Huye L, Bear A, Rooney CM, Wilson MH. Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model. PLoS ONE. 2015; 10(10): e0140744. PMID: 26473608, PMCID: PMC4608718, PII: PONE-D-15-17152, DOI: 10.1371/journal.pone.0140744, ISSN: 1932-6203.
Saito S, Nakazawa Y, Sueki A, Matsuda K, Tanaka M, Yanagisawa R, Maeda Y, Sato Y, Okabe S, Inukai T, Sugita K, Wilson MH, Rooney CM, Koike K. Anti-leukemic potency of piggyBac-mediated CD19-specific T cells against refractory Philadelphia chromosome-positive acute lymphoblastic leukemia. Cytotherapy. 2014 Sep; 16(9): 1257-69. PMID: 25108652, PII: S1465-3249(14)00640-9, DOI: 10.1016/j.jcyt.2014.05.022, ISSN: 1477-2566.
Galvan DL, Kettlun CS, Wilson MH. Targeting piggyBac transposon integrations in the human genome. Methods Mol. Biol. 2014; 1114: 143-61. PMID: 24557901, DOI: 10.1007/978-1-62703-761-7_9, ISSN: 1940-6029.
Doherty JE, Woodard LE, Bear AS, Foster AE, Wilson MH. An adaptable system for improving transposon-based gene expression in vivo via transient transgene repression. FASEB J [print-electronic]. 2013 Sep; 27(9): 3753-62. PMID: 23752206, PMCID: PMC3752539, PII: fj.13-232090, DOI: 10.1096/fj.13-232090, ISSN: 1530-6860.
Nakazawa Y, Saha S, Galvan DL, Huye L, Rollins L, Rooney CM, Wilson MH. Evaluation of long-term transgene expression in piggyBac-modified human T lymphocytes. J. Immunother. 2013 Jan; 36(1): 3-10. PMID: 23211626, PMCID: PMC3521868, DOI: 10.1097/CJI.0b013e3182791234, ISSN: 1537-4513.
Liang A, Wang Y, Woodard LE, Wilson MH, Sharma R, Awasthi YC, Du J, Mitch WE, Cheng J. Loss of glutathione S-transferase A4 accelerates obstruction-induced tubule damage and renal fibrosis. J. Pathol [print-electronic]. 2012 Dec; 228(4): 448-58. PMID: 22711583, PMCID: PMC3760987, DOI: 10.1002/path.4067, ISSN: 1096-9896.
Doherty JE, Huye LE, Yusa K, Zhou L, Craig NL, Wilson MH. Hyperactive piggyBac gene transfer in human cells and in vivo. Hum. Gene Ther [print-electronic]. 2012 Mar; 23(3): 311-20. PMID: 21992617, PMCID: PMC3300075, DOI: 10.1089/hum.2011.138, ISSN: 1557-7422.
Woodard LE, Li X, Malani N, Kaja A, Hice RH, Atkinson PW, Bushman FD, Craig NL, Wilson MH. Comparative analysis of the recently discovered hAT transposon TcBuster in human cells. PLoS ONE [print-electronic]. 2012; 7(11): e42666. PMID: 23166581, PMCID: PMC3499496, PII: PONE-D-12-05320, DOI: 10.1371/journal.pone.0042666, ISSN: 1932-6203.
Saha S, Nakazawa Y, Huye LE, Doherty JE, Galvan DL, Rooney CM, Wilson MH. PiggyBac transposon system modification of primary human T cells. J Vis Exp. 2012; (69): e4235. PMID: 23149543, PMCID: PMC3514050, PII: 4235, DOI: 10.3791/4235, ISSN: 1940-087X.
Huye LE, Nakazawa Y, Patel MP, Yvon E, Sun J, Savoldo B, Wilson MH, Dotti G, Rooney CM. Combining mTor inhibitors with rapamycin-resistant T cells: a two-pronged approach to tumor elimination. Mol. Ther [print-electronic]. 2011 Dec; 19(12): 2239-48. PMID: 21878902, PMCID: PMC3242659, PII: mt2011179, DOI: 10.1038/mt.2011.179, ISSN: 1525-0024.
Nakazawa Y, Huye LE, Salsman VS, Leen AM, Ahmed N, Rollins L, Dotti G, Gottschalk SM, Wilson MH, Rooney CM. PiggyBac-mediated cancer immunotherapy using EBV-specific cytotoxic T-cells expressing HER2-specific chimeric antigen receptor. Mol. Ther [print-electronic]. 2011 Dec; 19(12): 2133-43. PMID: 21772253, PMCID: PMC3242651, PII: mt2011131, DOI: 10.1038/mt.2011.131, ISSN: 1525-0024.
Kettlun C, Galvan DL, George AL, Kaja A, Wilson MH. Manipulating piggyBac transposon chromosomal integration site selection in human cells. Mol. Ther [print-electronic]. 2011 Sep; 19(9): 1636-44. PMID: 21730970, PMCID: PMC3182353, PII: mt2011129, DOI: 10.1038/mt.2011.129, ISSN: 1525-0024.
Wang J, Wang Y, Long J, Chang BH, Wilson MH, Overbeek P, Danesh FR. Tamoxifen-inducible podocyte-specific iCre recombinase transgenic mouse provides a simple approach for modulation of podocytes in vivo. Genesis. 2010 Jul; 48(7): 446-51. PMID: 20641128, PMCID: PMC3014324, DOI: 10.1002/dvg.20635, ISSN: 1526-968X.
Manuri PV, Wilson MH, Maiti SN, Mi T, Singh H, Olivares S, Dawson MJ, Huls H, Lee DA, Rao PH, Kaminski JM, Nakazawa Y, Gottschalk S, Kebriaei P, Shpall EJ, Champlin RE, Cooper LJ. PiggyBac transposon/transposase system to generate CD19-specific T cells for the treatment of B-lineage malignancies. Hum. Gene Ther. 2010 Apr; 21(4): 427-37. PMID: 19905893, PMCID: PMC2938363, DOI: 10.1089/hum.2009.114, ISSN: 1557-7422.
Kahlig KM, Saridey SK, Kaja A, Daniels MA, George AL, Wilson MH. Multiplexed transposon-mediated stable gene transfer in human cells. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2010 Jan 1/26/2010; 107(4): 1343-8. PMID: 20080581, PMCID: PMC2824351, PII: 0910383107, DOI: 10.1073/pnas.0910383107, ISSN: 1091-6490.
Wilson MH, George AL. Designing and testing chimeric zinc finger transposases. Methods Mol. Biol. 2010; 649: 353-63. PMID: 20680846, DOI: 10.1007/978-1-60761-753-2_22, ISSN: 1940-6029.
Saridey SK, Liu L, Doherty JE, Kaja A, Galvan DL, Fletcher BS, Wilson MH. PiggyBac transposon-based inducible gene expression in vivo after somatic cell gene transfer. Mol. Ther [print-electronic]. 2009 Dec; 17(12): 2115-20. PMID: 19809403, PMCID: PMC2814386, PII: mt2009234, DOI: 10.1038/mt.2009.234, ISSN: 1525-0024.
Nakazawa Y, Huye LE, Dotti G, Foster AE, Vera JF, Manuri PR, June CH, Rooney CM, Wilson MH. Optimization of the PiggyBac transposon system for the sustained genetic modification of human T lymphocytes. J. Immunother. 2009 Oct; 32(8): 826-36. PMID: 19752751, PMCID: PMC2796278, DOI: 10.1097/CJI.0b013e3181ad762b, ISSN: 1537-4513.
Galvan DL, Nakazawa Y, Kaja A, Kettlun C, Cooper LJ, Rooney CM, Wilson MH. Genome-wide mapping of PiggyBac transposon integrations in primary human T cells. J. Immunother. 2009 Oct; 32(8): 837-44. PMID: 19752750, PMCID: PMC2796288, DOI: 10.1097/CJI.0b013e3181b2914c, ISSN: 1537-4513.
Wilson MH, Coates CJ, George AL. PiggyBac transposon-mediated gene transfer in human cells. Mol. Ther. 2007 Jan; 15(1): 139-45. PMID: 17164785, PII: 6300028, DOI: 10.1038/sj.mt.6300028, ISSN: 1525-0024.
Wilson MH, Kaminski JM, George AL. Functional zinc finger/sleeping beauty transposase chimeras exhibit attenuated overproduction inhibition. FEBS Lett [print-electronic]. 2005 Nov 11/7/2005; 579(27): 6205-9. PMID: 16243318, PII: S0014-5793(05)01239-1, DOI: 10.1016/j.febslet.2005.10.004, ISSN: 0014-5793.
Johnson KB, Serwint JR, Fagan LM, Thompson RE, Wilson MH. Computer-based documentation: effect on parent and physician satisfaction during a pediatric health maintenance encounter. Arch Pediatr Adolesc Med. 2005 Mar; 159(3): 250-4. PMID: 15753268, PII: 159/3/250, DOI: 10.1001/archpedi.159.3.250, ISSN: 1072-4710.
O'Brochta DA, Sethuraman N, Wilson R, Hice RH, Pinkerton AC, Levesque CS, Bideshi DK, Jasinskiene N, Coates CJ, James AA, Lehane MJ, Atkinson PW. Gene vector and transposable element behavior in mosquitoes. J. Exp. Biol. 2003 Nov; 206(Pt 21): 3823-34. PMID: 14506218, ISSN: 0022-0949.
Tan CM, Wilson MH, MacMillan LB, Kobilka BK, Limbird LE. Heterozygous alpha 2A-adrenergic receptor mice unveil unique therapeutic benefits of partial agonists. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2002 Sep 9/17/2002; 99(19): 12471-6. PMID: 12205290, PMCID: PMC129469, PII: 122368499, DOI: 10.1073/pnas.122368499, ISSN: 0027-8424.
Wilson MH, Highfield HA, Limbird LE. The role of a conserved inter-transmembrane domain interface in regulating alpha(2a)-adrenergic receptor conformational stability and cell-surface turnover. Mol. Pharmacol. 2001 Apr; 59(4): 929-38. PMID: 11259639, ISSN: 0026-895X.
Wilson MH, Limbird LE. Mechanisms regulating the cell surface residence time of the alpha 2A-adrenergic receptor. Biochemistry. 2000 Feb 2/1/2000; 39(4): 693-700. PMID: 10651634, PII: bi9920275, ISSN: 0006-2960.
Wilson MH, Puranam RS, Ottman R, Gilliam C, Limbird LE, George AL, McNamara JO. Evaluation of the alpha(2A)-adrenergic receptor gene in a heritable form of temporal lobe epilepsy. Neurology. 1998 Dec; 51(6): 1730-1. PMID: 9855534, ISSN: 0028-3878.
Carty JS, Selvasingh J, Zuchowski Y, Nam HJ, Pénalva C, Nanayakkara G, Jennings EQ, Voss K, Adame ET, Tossberg JT, Yap WS, Melzer M, Viquez O, McCall AS, Piotrowski ER, Bessho R, Cao S, Leaptrot KL, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, Trapani JB, Cottam MA, Wan M, Shrivastava D, Delker DA, Wilson MH, Hasenour CM, Lantier L, Chernova I, Young JD, Haase VH, Vazquez-Medina JP, Kosma DK, Kim PK, Cartailler JP, Zhang M, Zent R, Harris RC, Watts JA, Terker AS, Bock F, Rathmell JC, Rodan AR, Arroyo JP. Dehydration promotes intracellular lipid synthesis and accumulation. Nat Commun. 2026 May 5/25/2026; 17(1): PMID: 42185304, PMCID: PMC13385796, PII: 10.1038/s41467-026-73534-x, DOI: 10.1038/s41467-026-73534-x, ISSN: 2041-1723.
Hickman PG, Asokan A, Wilson MH. Rewriting Renal Fate: The Evolving Landscape of Adeno-Associated Virus-Mediated Kidney Gene Therapies. Kidney360 [print-electronic]. 2026 Feb 2/1/2026; 7(2): 422-5. PMID: 41442198, PMCID: PMC12935359, PII: 02200512-202602000-00027, DOI: 10.34067/KID.0000001119, ISSN: 2641-7650.
Peek JL, Rosales A, Qi J, Feleke L, Menshikh A, Welch RC, Woodard LE, Asokan A, Wilson MH. Efficient kidney gene transfer and proximal tubule transduction using self-complementary AAV.cc47 vectors. Mol Ther Methods Clin Dev. 2025 Dec 12/11/2025; 33(4): 101606. PMID: 41146875, PMCID: PMC12554119, PII: S2329-0501(25)00201-3, DOI: 10.1016/j.omtm.2025.101606, ISSN: 2329-0501.
M Viquez O, Melzer M, Li S, Tantengco M, Dong X, Sha E, Huang J, Krystofiak ES, Hart RC, Luo W, Warren C, Bayazid AB, Zhang R, Bessho R, Haase VH, Brakebusch C, Inoue T, Brooks C, Wilson MH, Terker AS, Arroyo JP, Pozzi A, Zent R, Bock F. Rac1 promotes proximal tubule kidney repair by coupling the actin cytoskeleton to mitochondrial function. Proc Natl Acad Sci U S A [print-electronic]. 2025 Nov 11/25/2025; 122(47): e2504565122. PMID: 41248288, PMCID: PMC12626402, DOI: 10.1073/pnas.2504565122, ISSN: 1091-6490.
Woodard LE, Welch RC, Menshikh A, Luo W, Williams FM, Peek JL, Sha F, Veach RA, Kaja A, Beckermann TM, Ikizler TA, Wilson MH. Partial correction of cystinuria type A in mice via kidney-targeted transposon delivery. Mol Ther Nucleic Acids. 2025 Sep 9/9/2025; 36(3): 102639. PMID: 40791872, PMCID: PMC12337122, PII: S2162-2531(25)00193-3, DOI: 10.1016/j.omtn.2025.102639, ISSN: 2162-2531.
Bejoy J, Welch RC, Qian ES, Williams FM, Gibson-Corley KN, Wilson MH, Paragas N, Woodard LE. Urine-derived stem cells display homing, incorporation, and regeneration in human organoid and mouse models of acute kidney injury. Mol Ther [print-electronic]. 2025 Jul 7/2/2025; 33(7): 3307-20. PMID: 40158205, PMCID: PMC12266040, PII: S1525-0016(25)00219-9, DOI: 10.1016/j.ymthe.2025.03.044, ISSN: 1525-0024.
Rosales A, Blondel LO, Hull J, Gao Q, Aykun N, Peek JL, Vargas A, Fergione S, Song M, Wilson MH, Barbas AS, Asokan A. Evolving adeno-associated viruses for gene transfer to the kidney via cross-species cycling of capsid libraries. Nat Biomed Eng [print-electronic]. 2025 Jul; 9(7): 1086-100. PMID: 39910375, PMCID: PMC12261118, PII: 10.1038/s41551-024-01341-0, DOI: 10.1038/s41551-024-01341-0, ISSN: 2157-846X.
Asokan A, Wilson MH. Filtering through AAV capsid libraries for effective kidney gene transfer. Kidney Int [print-electronic]. 2025 Apr; 107(4): 591-3. PMID: 39814263, PII: S0085-2538(25)00014-6, DOI: 10.1016/j.kint.2024.12.012, ISSN: 1523-1755.
Hickman AB, Lannes L, Furman CM, Hong C, Franklin L, Ghirlando R, Ghosh A, Luo W, Konstantinidou P, Lorenzi HA, Grove A, Haase AD, Wilson MH, Dyda F. Activity of the mammalian DNA transposon piggyBat from Myotis lucifugus is restricted by its own transposon ends. Nat Commun. 2025 Jan 1/7/2025; 16(1): 458. PMID: 39774116, PMCID: PMC11707139, PII: 10.1038/s41467-024-55784-9, DOI: 10.1038/s41467-024-55784-9, ISSN: 2041-1723.
Cao S, Pan Y, Terker AS, Arroyo Ornelas JP, Wang Y, Tang J, Niu A, Kar SA, Jiang M, Luo W, Dong X, Fan X, Wang S, Wilson MH, Fogo A, Zhang MZ, Harris RC. Epidermal growth factor receptor activation is essential for kidney fibrosis development. Nat Commun. 2023 Nov 11/14/2023; 14(1): 7357. PMID: 37963889, PMCID: PMC10645887, PII: 10.1038/s41467-023-43226-x, DOI: 10.1038/s41467-023-43226-x, ISSN: 2041-1723.
Peek JL, Wilson MH. Cell and gene therapy for kidney disease. Nat Rev Nephrol [print-electronic]. 2023 Jul; 19(7): 451-62. PMID: 36973494, PMCID: PMC10339687, PII: 10.1038/s41581-023-00702-3, DOI: 10.1038/s41581-023-00702-3, ISSN: 1759-507X.
Kenigsberg Z, Welch RC, Bejoy J, Williams FM, Veach RA, Jarrett I, Thompson TK, Wilson MH, Woodard LE. Genome Engineering of Human Urine-Derived Stem Cells to Express Lactoferrin and Deoxyribonuclease. Tissue Eng Part A [print-electronic]. 2023 Jul; 29(13-14): 372-83. PMID: 37130035, PMCID: PMC10354709, DOI: 10.1089/ten.TEA.2023.0003, ISSN: 1937-335X.
Pan Y, Cao S, Terker AS, Tang J, Sasaki K, Wang Y, Niu A, Luo W, Daassi D, Fan X, Wang S, Wilson MH, Zhang MZ, Harris RC. Corrigendum to "Myeloid cyclooxygenase-2/prostaglandin E2/E-type prostanoid receptor 4 promotes transcription factor MafB-dependent inflammatory resolution in acute kidney injury." Kidney Int. 2022;101:79-91. Kidney Int. 2023 Apr; 103(4): 798. PMID: 36948771, PII: S0085-2538(23)00062-5, DOI: 10.1016/j.kint.2023.01.014, ISSN: 1523-1755.
Hunter K, Larsen JA, Love HD, Evans RC, Roy S, Zent R, Harris RC, Wilson MH, Fissell WH. Inhibition of Transforming Growth Factor-ß Improves Primary Renal Tubule Cell Differentiation in Long-Term Culture. Tissue Eng Part A [print-electronic]. 2023 Feb; 29(3-4): 102-11. PMID: 36274231, PMCID: PMC10081716, DOI: 10.1089/ten.TEA.2022.0147, ISSN: 1937-335X.
Pan Y, Cao S, Terker AS, Tang J, Sasaki K, Wang Y, Niu A, Luo W, Daasi D, Fan X, Wang S, Wilson MH, Zhang MZ, Harris RC. Corrigendum to "Myeloid cyclooxygenase-2/prostaglandin E2/E-type prostanoid receptor 4 promotes transcription factor MafB-dependent inflammatory resolution in acute kidney injury." Kidney Int. 2022;101:79-91. Kidney Int. 2023 Jan; 103(1): 229. PMID: 36603974, PMCID: PMC10003615, PII: S0085-2538(22)00909-7, DOI: 10.1016/j.kint.2022.10.005, ISSN: 1523-1755.
Arroyo JP, Terker AS, Zuchowski Y, Watts JA, Bock F, Meyer C, Luo W, Kapp ME, Gould ER, Miranda AX, Carty J, Jiang M, Vanacore RM, Hammock E, Wilson MH, Zent R, Zhang M, Bhave G, Harris RC. Kidney collecting duct cells make vasopressin in response to NaCl-induced hypertonicity. JCI Insight. 2022 Dec 12/22/2022; 7(24): PMID: 36326835, PMCID: PMC9869977, PII: 161765, DOI: 10.1172/jci.insight.161765, ISSN: 2379-3708.
Luo W, Hickman AB, Genzor P, Ghirlando R, Furman CM, Menshikh A, Haase A, Dyda F, Wilson MH. Transposase N-terminal phosphorylation and asymmetric transposon ends inhibit piggyBac transposition in mammalian cells. Nucleic Acids Res. 2022 Dec 12/9/2022; 50(22): 13128-42. PMID: 36537219, PMCID: PMC9825180, PII: 6946998, DOI: 10.1093/nar/gkac1191, ISSN: 1362-4962.
Haake SM, Plosa EJ, Kropski JA, Venton LA, Reddy A, Bock F, Chang BT, Luna AJ, Nabukhotna K, Xu ZQ, Prather RA, Lee S, Tanjore H, Polosukhin VV, Viquez OM, Jones A, Luo W, Wilson MH, Rathmell WK, Massion PP, Pozzi A, Blackwell TS, Zent R. Ligand-independent integrin ß1 signaling supports lung adenocarcinoma development. JCI Insight. 2022 Aug 8/8/2022; 7(15): PMID: 35763345, PMCID: PMC9462485, PII: 154098, DOI: 10.1172/jci.insight.154098, ISSN: 2379-3708.
Peek JL, Wilson MH. Gene therapy for kidney disease: targeting cystinuria. Curr Opin Nephrol Hypertens. 2022 Mar 3/1/2022; 31(2): 175-9. PMID: 34982522, PMCID: PMC8799525, PII: 00041552-202203000-00008, DOI: 10.1097/MNH.0000000000000768, ISSN: 1473-6543.
Pan Y, Cao S, Terker AS, Tang J, Sasaki K, Wang Y, Niu A, Luo W, Fan X, Wang S, Wilson MH, Zhang MZ, Harris RC. Myeloid cyclooxygenase-2/prostaglandin E2/E-type prostanoid receptor 4 promotes transcription factor MafB-dependent inflammatory resolution in acute kidney injury. Kidney Int [print-electronic]. 2022 Jan; 101(1): 79-91. PMID: 34774558, PMCID: PMC8741730, PII: S0085-2538(21)01032-2, DOI: 10.1016/j.kint.2021.09.033, ISSN: 1523-1755.
Wilson MH, Gottschalk S. Expect the unexpected: piggyBac and lymphoma [editorial]. Blood. 2021 Oct 10/21/2021; 138(16): 1379-80. PMID: 34673949, PII: S0006-4971(21)01729-8, DOI: 10.1182/blood.2021012349, ISSN: 1528-0020.
Beckermann TM, Luo W, Wilson CM, Veach RA, Wilson MH. Cognate restriction of transposition by piggyBac-like proteins. Nucleic Acids Res. 2021 Aug 8/20/2021; 49(14): 8135-44. PMID: 34232995, PMCID: PMC8373079, PII: 6316838, DOI: 10.1093/nar/gkab578, ISSN: 1362-4962.
Reinfeld BI, Madden MZ, Wolf MM, Chytil A, Bader JE, Patterson AR, Sugiura A, Cohen AS, Ali A, Do BT, Muir A, Lewis CA, Hongo RA, Young KL, Brown RE, Todd VM, Huffstater T, Abraham A, O'Neil RT, Wilson MH, Xin F, Tantawy MN, Merryman WD, Johnson RW, Williams CS, Mason EF, Mason FM, Beckermann KE, Vander Heiden MG, Manning HC, Rathmell JC, Rathmell WK. Cell-programmed nutrient partitioning in the tumour microenvironment. Nature [print-electronic]. 2021 May; 593(7858): 282-8. PMID: 33828302, PMCID: PMC8122068, PII: 10.1038/s41586-021-03442-1, DOI: 10.1038/s41586-021-03442-1, ISSN: 1476-4687.
Woodard LE, Williams FM, Jarrett IC, Wilson MH. Functional analysis of the catalytic triad of the hAT-family transposase TcBuster. Plasmid [print-electronic]. 2021 Mar; 114: 102554. PMID: 33476638, PMCID: PMC7946762, PII: S0147-619X(21)00001-9, DOI: 10.1016/j.plasmid.2021.102554, ISSN: 1095-9890.
Love H, Evans R, Humes HD, Roy S, Zent R, Harris R, Wilson M, Fissell WH. Metformin and Inhibition of Transforming Growth Factor-Beta Stimulate In Vitro Transport in Primary Renal Tubule Cells. Tissue Eng Part A. 2020 Oct; 26(19-20): 1091-8. PMID: 32312181, PMCID: PMC7580646, DOI: 10.1089/ten.TEA.2019.0294, ISSN: 1937-335X.
Chiusa M, Hu W, Zienkiewicz J, Chen X, Zhang MZ, Harris RC, Vanacore RM, Bentz JA, Remuzzi G, Benigni A, Fogo AB, Luo W, Mili S, Wilson MH, Zent R, Hawiger J, Pozzi A. EGF receptor-mediated FUS phosphorylation promotes its nuclear translocation and fibrotic signaling. J Cell Biol. 2020 Sep 9/7/2020; 219(9): PMID: 32678881, PMCID: PMC7480104, PII: 151955, DOI: 10.1083/jcb.202001120, ISSN: 1540-8140.
Chen Q, Luo W, Veach RA, Hickman AB, Wilson MH, Dyda F. Structural basis of seamless excision and specific targeting by piggyBac transposase. Nat Commun. 2020 Jul 7/10/2020; 11(1): 3446. PMID: 32651359, PMCID: PMC7351741, PII: 10.1038/s41467-020-17128-1, DOI: 10.1038/s41467-020-17128-1, ISSN: 2041-1723.
Beckermann TM, Welch RC, Williams FM, Mortlock DP, Sha F, Ikizler TA, Woodard LE, Wilson MH. CRISPR/Cas9 engineering of albino cystinuria Type A mice. Genesis [print-electronic]. 2020 May; 58(5): e23357. PMID: 32078250, DOI: 10.1002/dvg.23357, ISSN: 1526-968X.
Wilson MH, Veach RA, Luo W, Welch RC, Roy S, Fissell WH. Genome Engineering Renal Epithelial Cells for Enhanced Volume Transport Function. Cell Mol Bioeng. 2020 Feb; 13(1): 17-26. PMID: 32030105, PMCID: PMC6981331, PII: 601, DOI: 10.1007/s12195-019-00601-3, ISSN: 1865-5025.
Woodard LE, Welch RC, Veach RA, Beckermann TM, Sha F, Weinman EJ, Ikizler TA, Tischfield JA, Sahota A, Wilson MH. Metabolic consequences of cystinuria. BMC Nephrol. 2019 Jun 6/20/2019; 20(1): 227. PMID: 31221135, PMCID: PMC6585015, PII: 10.1186/s12882-019-1417-8, DOI: 10.1186/s12882-019-1417-8, ISSN: 1471-2369.
Vanslambrouck JM, Woodard LE, Suhaimi N, Williams FM, Howden SE, Wilson SB, Lonsdale A, Er PX, Li J, Maksimovic J, Oshlack A, Wilson MH, Little MH. Direct reprogramming to human nephron progenitor-like cells using inducible piggyBac transposon expression of SNAI2-EYA1-SIX1. Kidney Int [print-electronic]. 2019 May; 95(5): 1153-66. PMID: 30827514, PMCID: PMC6478531, PII: S0085-2538(19)30038-9, DOI: 10.1016/j.kint.2018.11.041, ISSN: 1523-1755.
Wilson MH. Consider Changing the Horse for Your CAR-T?. Mol. Ther [print-electronic]. 2018 Aug 8/1/2018; 26(8): 1873-4. PMID: 30078438, PMCID: PMC6094485, PII: S1525-0016(18)30277-6, DOI: 10.1016/j.ymthe.2018.06.018, ISSN: 1525-0024.
Hamada M, Nishio N, Okuno Y, Suzuki S, Kawashima N, Muramatsu H, Tsubota S, Wilson MH, Morita D, Kataoka S, Ichikawa D, Murakami N, Taniguchi R, Suzuki K, Kojima D, Sekiya Y, Nishikawa E, Narita A, Hama A, Kojima S, Nakazawa Y, Takahashi Y. Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR. EBioMedicine [print-electronic]. 2018 Aug; 34: 18-26. PMID: 30082227, PMCID: PMC6116345, PII: S2352-3964(18)30251-2, DOI: 10.1016/j.ebiom.2018.07.008, ISSN: 2352-3964.
O'Neil RT, Saha S, Veach RA, Welch RC, Woodard LE, Rooney CM, Wilson MH. Transposon-modified antigen-specific T lymphocytes for sustained therapeutic protein delivery in vivo. Nat Commun. 2018 Apr 4/10/2018; 9(1): 1325. PMID: 29636469, PMCID: PMC5893599, PII: 10.1038/s41467-018-03787-8, DOI: 10.1038/s41467-018-03787-8, ISSN: 2041-1723.
Morita D, Nishio N, Saito S, Tanaka M, Kawashima N, Okuno Y, Suzuki S, Matsuda K, Maeda Y, Wilson MH, Dotti G, Rooney CM, Takahashi Y, Nakazawa Y. Enhanced Expression of Anti-CD19 Chimeric Antigen Receptor in piggyBac Transposon-Engineered T Cells. Mol Ther Methods Clin Dev. 2018 Mar 3/16/2018; 8: 131-40. PMID: 29687032, PMCID: PMC5907825, PII: S2329-0501(17)30128-6, DOI: 10.1016/j.omtm.2017.12.003, ISSN: 2329-0501.
Woodard LE, Welch RC, Williams FM, Luo W, Cheng J, Wilson MH. Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney. J Vis Exp. 2018 Jan 1/8/2018; (131): PMID: 29364221, PMCID: PMC5907682, DOI: 10.3791/56324, ISSN: 1940-087X.
Veach RA, Wilson MH. CRISPR/Cas9 engineering of a KIM-1 reporter human proximal tubule cell line. PLoS ONE. 2018; 13(9): e0204487. PMID: 30260998, PMCID: PMC6160069, PII: PONE-D-17-41126, DOI: 10.1371/journal.pone.0204487, ISSN: 1932-6203.
Luo W, Galvan DL, Woodard LE, Dorset D, Levy S, Wilson MH. Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells. Nucleic Acids Res. 2017 Aug 8/21/2017; 45(14): 8411-22. PMID: 28666380, PII: 3897173, DOI: 10.1093/nar/gkx572, ISSN: 1362-4962.
Woodard LE, Cheng J, Welch RC, Williams FM, Luo W, Gewin LS, Wilson MH. Kidney-specific transposon-mediated gene transfer in vivo. Sci Rep. 2017 Mar 3/20/2017; 7: 44904. PMID: 28317878, PMCID: PMC5357952, PII: srep44904, DOI: 10.1038/srep44904, ISSN: 2045-2322.
Woodard LE, Downes LM, Lee YC, Kaja A, Terefe ES, Wilson MH. Temporal self-regulation of transposition through host-independent transposase rodlet formation. Nucleic Acids Res [print-electronic]. 2017 Jan 1/9/2017; 45(1): 353-66. PMID: 27899587, PMCID: PMC5224482, PII: gkw1115, DOI: 10.1093/nar/gkw1115, ISSN: 1362-4962.
Held EP, Iglesia EA, Johnson AS, Fang JY, Wilson MH, Abel TW, Lake W, Siew ED. Neurosarcoidosis Presenting as Aseptic Meningitis in an Immunosuppressed Renal Transplant Recipient. Transplantation. 2016 Oct; 100(10): e96-e100. PMID: 26863472, DOI: 10.1097/TP.0000000000001074, ISSN: 1534-6080.
Nakazawa Y, Matsuda K, Kurata T, Sueki A, Tanaka M, Sakashita K, Imai C, Wilson MH, Koike K. Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34(+) cells of juvenile myelomonocytic leukemia. J Hematol Oncol. 2016 Mar 3/16/2016; 9: 27. PMID: 26983639, PMCID: PMC4793548, PII: 10.1186/s13045-016-0256-3, DOI: 10.1186/s13045-016-0256-3, ISSN: 1756-8722.
Woodard LE, Wilson MH. PiggyBac-ing models and new therapeutic strategies. Trends Biotechnol [print-electronic]. 2015 Sep; 33(9): 525-33. PMID: 26211958, PMCID: PMC4663986, PII: S0167-7799(15)00137-7, DOI: 10.1016/j.tibtech.2015.06.009, ISSN: 1879-3096.
Liang M, Woodard LE, Liang A, Luo J, Wilson MH, Mitch WE, Cheng J. Protective role of insulin-like growth factor-1 receptor in endothelial cells against unilateral ureteral obstruction-induced renal fibrosis. Am. J. Pathol [print-electronic]. 2015 May; 185(5): 1234-50. PMID: 25783760, PMCID: PMC4419212, PII: S0002-9440(15)00126-1, DOI: 10.1016/j.ajpath.2015.01.027, ISSN: 1525-2191.
Saha S, Woodard LE, Charron EM, Welch RC, Rooney CM, Wilson MH. Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells. Nucleic Acids Res [print-electronic]. 2015 Feb 2/18/2015; 43(3): 1770-82. PMID: 25605795, PMCID: PMC4330379, PII: gkv017, DOI: 10.1093/nar/gkv017, ISSN: 1362-4962.
Galvan DL, O'Neil RT, Foster AE, Huye L, Bear A, Rooney CM, Wilson MH. Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model. PLoS ONE. 2015; 10(10): e0140744. PMID: 26473608, PMCID: PMC4608718, PII: PONE-D-15-17152, DOI: 10.1371/journal.pone.0140744, ISSN: 1932-6203.
Saito S, Nakazawa Y, Sueki A, Matsuda K, Tanaka M, Yanagisawa R, Maeda Y, Sato Y, Okabe S, Inukai T, Sugita K, Wilson MH, Rooney CM, Koike K. Anti-leukemic potency of piggyBac-mediated CD19-specific T cells against refractory Philadelphia chromosome-positive acute lymphoblastic leukemia. Cytotherapy. 2014 Sep; 16(9): 1257-69. PMID: 25108652, PII: S1465-3249(14)00640-9, DOI: 10.1016/j.jcyt.2014.05.022, ISSN: 1477-2566.
Galvan DL, Kettlun CS, Wilson MH. Targeting piggyBac transposon integrations in the human genome. Methods Mol. Biol. 2014; 1114: 143-61. PMID: 24557901, DOI: 10.1007/978-1-62703-761-7_9, ISSN: 1940-6029.
Doherty JE, Woodard LE, Bear AS, Foster AE, Wilson MH. An adaptable system for improving transposon-based gene expression in vivo via transient transgene repression. FASEB J [print-electronic]. 2013 Sep; 27(9): 3753-62. PMID: 23752206, PMCID: PMC3752539, PII: fj.13-232090, DOI: 10.1096/fj.13-232090, ISSN: 1530-6860.
Nakazawa Y, Saha S, Galvan DL, Huye L, Rollins L, Rooney CM, Wilson MH. Evaluation of long-term transgene expression in piggyBac-modified human T lymphocytes. J. Immunother. 2013 Jan; 36(1): 3-10. PMID: 23211626, PMCID: PMC3521868, DOI: 10.1097/CJI.0b013e3182791234, ISSN: 1537-4513.
Liang A, Wang Y, Woodard LE, Wilson MH, Sharma R, Awasthi YC, Du J, Mitch WE, Cheng J. Loss of glutathione S-transferase A4 accelerates obstruction-induced tubule damage and renal fibrosis. J. Pathol [print-electronic]. 2012 Dec; 228(4): 448-58. PMID: 22711583, PMCID: PMC3760987, DOI: 10.1002/path.4067, ISSN: 1096-9896.
Doherty JE, Huye LE, Yusa K, Zhou L, Craig NL, Wilson MH. Hyperactive piggyBac gene transfer in human cells and in vivo. Hum. Gene Ther [print-electronic]. 2012 Mar; 23(3): 311-20. PMID: 21992617, PMCID: PMC3300075, DOI: 10.1089/hum.2011.138, ISSN: 1557-7422.
Woodard LE, Li X, Malani N, Kaja A, Hice RH, Atkinson PW, Bushman FD, Craig NL, Wilson MH. Comparative analysis of the recently discovered hAT transposon TcBuster in human cells. PLoS ONE [print-electronic]. 2012; 7(11): e42666. PMID: 23166581, PMCID: PMC3499496, PII: PONE-D-12-05320, DOI: 10.1371/journal.pone.0042666, ISSN: 1932-6203.
Saha S, Nakazawa Y, Huye LE, Doherty JE, Galvan DL, Rooney CM, Wilson MH. PiggyBac transposon system modification of primary human T cells. J Vis Exp. 2012; (69): e4235. PMID: 23149543, PMCID: PMC3514050, PII: 4235, DOI: 10.3791/4235, ISSN: 1940-087X.
Huye LE, Nakazawa Y, Patel MP, Yvon E, Sun J, Savoldo B, Wilson MH, Dotti G, Rooney CM. Combining mTor inhibitors with rapamycin-resistant T cells: a two-pronged approach to tumor elimination. Mol. Ther [print-electronic]. 2011 Dec; 19(12): 2239-48. PMID: 21878902, PMCID: PMC3242659, PII: mt2011179, DOI: 10.1038/mt.2011.179, ISSN: 1525-0024.
Nakazawa Y, Huye LE, Salsman VS, Leen AM, Ahmed N, Rollins L, Dotti G, Gottschalk SM, Wilson MH, Rooney CM. PiggyBac-mediated cancer immunotherapy using EBV-specific cytotoxic T-cells expressing HER2-specific chimeric antigen receptor. Mol. Ther [print-electronic]. 2011 Dec; 19(12): 2133-43. PMID: 21772253, PMCID: PMC3242651, PII: mt2011131, DOI: 10.1038/mt.2011.131, ISSN: 1525-0024.
Kettlun C, Galvan DL, George AL, Kaja A, Wilson MH. Manipulating piggyBac transposon chromosomal integration site selection in human cells. Mol. Ther [print-electronic]. 2011 Sep; 19(9): 1636-44. PMID: 21730970, PMCID: PMC3182353, PII: mt2011129, DOI: 10.1038/mt.2011.129, ISSN: 1525-0024.
Wang J, Wang Y, Long J, Chang BH, Wilson MH, Overbeek P, Danesh FR. Tamoxifen-inducible podocyte-specific iCre recombinase transgenic mouse provides a simple approach for modulation of podocytes in vivo. Genesis. 2010 Jul; 48(7): 446-51. PMID: 20641128, PMCID: PMC3014324, DOI: 10.1002/dvg.20635, ISSN: 1526-968X.
Manuri PV, Wilson MH, Maiti SN, Mi T, Singh H, Olivares S, Dawson MJ, Huls H, Lee DA, Rao PH, Kaminski JM, Nakazawa Y, Gottschalk S, Kebriaei P, Shpall EJ, Champlin RE, Cooper LJ. PiggyBac transposon/transposase system to generate CD19-specific T cells for the treatment of B-lineage malignancies. Hum. Gene Ther. 2010 Apr; 21(4): 427-37. PMID: 19905893, PMCID: PMC2938363, DOI: 10.1089/hum.2009.114, ISSN: 1557-7422.
Kahlig KM, Saridey SK, Kaja A, Daniels MA, George AL, Wilson MH. Multiplexed transposon-mediated stable gene transfer in human cells. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2010 Jan 1/26/2010; 107(4): 1343-8. PMID: 20080581, PMCID: PMC2824351, PII: 0910383107, DOI: 10.1073/pnas.0910383107, ISSN: 1091-6490.
Wilson MH, George AL. Designing and testing chimeric zinc finger transposases. Methods Mol. Biol. 2010; 649: 353-63. PMID: 20680846, DOI: 10.1007/978-1-60761-753-2_22, ISSN: 1940-6029.
Saridey SK, Liu L, Doherty JE, Kaja A, Galvan DL, Fletcher BS, Wilson MH. PiggyBac transposon-based inducible gene expression in vivo after somatic cell gene transfer. Mol. Ther [print-electronic]. 2009 Dec; 17(12): 2115-20. PMID: 19809403, PMCID: PMC2814386, PII: mt2009234, DOI: 10.1038/mt.2009.234, ISSN: 1525-0024.
Nakazawa Y, Huye LE, Dotti G, Foster AE, Vera JF, Manuri PR, June CH, Rooney CM, Wilson MH. Optimization of the PiggyBac transposon system for the sustained genetic modification of human T lymphocytes. J. Immunother. 2009 Oct; 32(8): 826-36. PMID: 19752751, PMCID: PMC2796278, DOI: 10.1097/CJI.0b013e3181ad762b, ISSN: 1537-4513.
Galvan DL, Nakazawa Y, Kaja A, Kettlun C, Cooper LJ, Rooney CM, Wilson MH. Genome-wide mapping of PiggyBac transposon integrations in primary human T cells. J. Immunother. 2009 Oct; 32(8): 837-44. PMID: 19752750, PMCID: PMC2796288, DOI: 10.1097/CJI.0b013e3181b2914c, ISSN: 1537-4513.
Wilson MH, Coates CJ, George AL. PiggyBac transposon-mediated gene transfer in human cells. Mol. Ther. 2007 Jan; 15(1): 139-45. PMID: 17164785, PII: 6300028, DOI: 10.1038/sj.mt.6300028, ISSN: 1525-0024.
Wilson MH, Kaminski JM, George AL. Functional zinc finger/sleeping beauty transposase chimeras exhibit attenuated overproduction inhibition. FEBS Lett [print-electronic]. 2005 Nov 11/7/2005; 579(27): 6205-9. PMID: 16243318, PII: S0014-5793(05)01239-1, DOI: 10.1016/j.febslet.2005.10.004, ISSN: 0014-5793.
Johnson KB, Serwint JR, Fagan LM, Thompson RE, Wilson MH. Computer-based documentation: effect on parent and physician satisfaction during a pediatric health maintenance encounter. Arch Pediatr Adolesc Med. 2005 Mar; 159(3): 250-4. PMID: 15753268, PII: 159/3/250, DOI: 10.1001/archpedi.159.3.250, ISSN: 1072-4710.
O'Brochta DA, Sethuraman N, Wilson R, Hice RH, Pinkerton AC, Levesque CS, Bideshi DK, Jasinskiene N, Coates CJ, James AA, Lehane MJ, Atkinson PW. Gene vector and transposable element behavior in mosquitoes. J. Exp. Biol. 2003 Nov; 206(Pt 21): 3823-34. PMID: 14506218, ISSN: 0022-0949.
Tan CM, Wilson MH, MacMillan LB, Kobilka BK, Limbird LE. Heterozygous alpha 2A-adrenergic receptor mice unveil unique therapeutic benefits of partial agonists. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2002 Sep 9/17/2002; 99(19): 12471-6. PMID: 12205290, PMCID: PMC129469, PII: 122368499, DOI: 10.1073/pnas.122368499, ISSN: 0027-8424.
Wilson MH, Highfield HA, Limbird LE. The role of a conserved inter-transmembrane domain interface in regulating alpha(2a)-adrenergic receptor conformational stability and cell-surface turnover. Mol. Pharmacol. 2001 Apr; 59(4): 929-38. PMID: 11259639, ISSN: 0026-895X.
Wilson MH, Limbird LE. Mechanisms regulating the cell surface residence time of the alpha 2A-adrenergic receptor. Biochemistry. 2000 Feb 2/1/2000; 39(4): 693-700. PMID: 10651634, PII: bi9920275, ISSN: 0006-2960.
Wilson MH, Puranam RS, Ottman R, Gilliam C, Limbird LE, George AL, McNamara JO. Evaluation of the alpha(2A)-adrenergic receptor gene in a heritable form of temporal lobe epilepsy. Neurology. 1998 Dec; 51(6): 1730-1. PMID: 9855534, ISSN: 0028-3878.
Available Postdoctoral Position Details
Posted: 8/29/2022
NIH funded postdoctoral positions are available for research in developing cell and gene therapies for kidney diseases. Individuals with a strong background in molecular biology, cell culture, and a willingness to work with animals are encouraged to apply. The researcher will learn a variety of novel techniques for in vivo and ex vivo gene transfer including transposon technology, CRISPR/Cas9, and adeno-associated viral (AAV) vectors. Current projects are focused on developing a cell therapy for Fabry disease, engineering new AAV vectors, and advancing non-viral genome engineering and gene delivery technologies.
Interested applicants can contact Dr. Wilson directly for consideration at matthew.wilson@vumc.org