Faculty Appointments
Research Instructor in Medicine
Education
Ph.D., Mississippi State University, Starkville, MississippiM.S., Mississippi State University, Starkville, MississippiB.S., Mississippi State University, Starkville, Mississippi
Office Address
4140 MRBIII
TN 8355
TN 8355
Publications
Shah AT, Cannon TM, Higginbotham JN, Coffey RJ, Skala MC. Autofluorescence flow sorting of breast cancer cell metabolism. J Biophotonics [print-electronic]. 2017 Aug; 10(8): 1026-33. PMID: 27730745, PMCID: PMC5547001, DOI: 10.1002/jbio.201600128, ISSN: 1864-0648.
Dou Y, Cha DJ, Franklin JL, Higginbotham JN, Jeppesen DK, Weaver AM, Prasad N, Levy S, Coffey RJ, Patton JG, Zhang B. Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes. Sci Rep. 2016 Nov 11/28/2016; 6: 37982. PMID: 27892494, PMCID: PMC5125100, PII: srep37982, DOI: 10.1038/srep37982, ISSN: 2045-2322.
Higginbotham JN, Zhang Q, Jeppesen DK, Scott AM, Manning HC, Ochieng J, Franklin JL, Coffey RJ. Identification and characterization of EGF receptor in individual exosomes by fluorescence-activated vesicle sorting. J Extracell Vesicles. 2016; 5: 29254. PMID: 27345057, PMCID: PMC4921784, PII: 29254, ISSN: 2001-3078.
Cha DJ, Franklin JL, Dou Y, Liu Q, Higginbotham JN, Demory Beckler M, Weaver AM, Vickers K, Prasad N, Levy S, Zhang B, Coffey RJ, Patton JG. KRAS-dependent sorting of miRNA to exosomes. Elife. 2015 Jul 7/1/2015; 4: e07197. PMID: 26132860, PMCID: PMC4510696, DOI: 10.7554/eLife.07197, ISSN: 2050-084X.
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Mol. Cell Proteomics [print-electronic]. 2013 Feb; 12(2): 343-55. PMID: 23161513, PMCID: PMC3567858, PII: M112.022806, DOI: 10.1074/mcp.M112.022806, ISSN: 1535-9484.
Shifrin DA, McConnell RE, Nambiar R, Higginbotham JN, Coffey RJ, Tyska MJ. Enterocyte microvillus-derived vesicles detoxify bacterial products and regulate epithelial-microbial interactions. Curr. Biol [print-electronic]. 2012 Apr 4/10/2012; 22(7): 627-31. PMID: 22386311, PMCID: PMC3326206, PII: S0960-9822(12)00171-6, DOI: 10.1016/j.cub.2012.02.022, ISSN: 1879-0445.
Powell AE, Wang Y, Li Y, Poulin EJ, Means AL, Washington MK, Higginbotham JN, Juchheim A, Prasad N, Levy SE, Guo Y, Shyr Y, Aronow BJ, Haigis KM, Franklin JL, Coffey RJ. The pan-ErbB negative regulator Lrig1 is an intestinal stem cell marker that functions as a tumor suppressor. Cell. 2012 Mar 3/30/2012; 149(1): 146-58. PMID: 22464327, PMCID: PMC3563328, PII: S0092-8674(12)00280-2, DOI: 10.1016/j.cell.2012.02.042, ISSN: 1097-4172.
Gephart JD, Singh B, Higginbotham JN, Franklin JL, Gonzalez A, Fölsch H, Coffey RJ. Identification of a novel mono-leucine basolateral sorting motif within the cytoplasmic domain of amphiregulin. Traffic [print-electronic]. 2011 Dec; 12(12): 1793-804. PMID: 21917092, PMCID: PMC3886124, DOI: 10.1111/j.1600-0854.2011.01282.x, ISSN: 1600-0854.
Higginbotham JN, Demory Beckler M, Gephart JD, Franklin JL, Bogatcheva G, Kremers GJ, Piston DW, Ayers GD, McConnell RE, Tyska MJ, Coffey RJ. Amphiregulin exosomes increase cancer cell invasion. Curr. Biol [print-electronic]. 2011 May 5/10/2011; 21(9): 779-86. PMID: 21514161, PMCID: PMC3417320, PII: S0960-9822(11)00347-2, DOI: 10.1016/j.cub.2011.03.043, ISSN: 1879-0445.
Lapierre LA, Caldwell CM, Higginbotham JN, Avant KM, Hall J, Beauchamp RD, Goldenring JR. Transformation of rat intestinal epithelial cells by overexpression of Rab25 is microtubule dependent. Cytoskeleton (Hoboken) [print-electronic]. 2011 Feb; 68(2): 97-111. PMID: 21246754, PMCID: PMC3885764, DOI: 10.1002/cm.20497, ISSN: 1949-3592.
McConnell RE, Higginbotham JN, Shifrin DA, Tabb DL, Coffey RJ, Tyska MJ. The enterocyte microvillus is a vesicle-generating organelle. J. Cell Biol. 2009 Jun 6/29/2009; 185(7): 1285-98. PMID: 19564407, PMCID: PMC2712962, PII: jcb.200902147, DOI: 10.1083/jcb.200902147, ISSN: 1540-8140.
Cao Z, Li C, Higginbotham JN, Franklin JL, Tabb DL, Graves-Deal R, Hill S, Cheek K, Jerome WG, Lapierre LA, Goldenring JR, Ham AJ, Coffey RJ. Use of fluorescence-activated vesicle sorting for isolation of Naked2-associated, basolaterally targeted exocytic vesicles for proteomics analysis. Mol. Cell Proteomics [print-electronic]. 2008 Sep; 7(9): 1651-67. PMID: 18504258, PMCID: PMC2528074, PII: M700155-MCP200, DOI: 10.1074/mcp.M700155-MCP200, ISSN: 1535-9484.
Tian C, Luskin GK, Dischert KM, Higginbotham JN, Shepherd BE, Crowe JE. Immunodominance of the VH1-46 antibody gene segment in the primary repertoire of human rotavirus-specific B cells is reduced in the memory compartment through somatic mutation of nondominant clones. J. Immunol. 2008 Mar 3/1/2008; 180(5): 3279-88. PMID: 18292552, PII: 180/5/3279, ISSN: 0022-1767.
Hartig SM, Greene RR, Carlesso G, Higginbotham JN, Khan WN, Prokop A, Davidson JM. Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells. Biomaterials [print-electronic]. 2007 Sep; 28(26): 3843-55. PMID: 17560645, PMCID: PMC2000344, PII: S0142-9612(07)00346-8, DOI: 10.1016/j.biomaterials.2007.04.027, ISSN: 0142-9612.
Moore ML, Chi MH, Zhou W, Goleniewska K, O'Neal JF, Higginbotham JN, Peebles RS. Cutting Edge: Oseltamivir decreases T cell GM1 expression and inhibits clearance of respiratory syncytial virus: potential role of endogenous sialidase in antiviral immunity. J. Immunol. 2007 Mar 3/1/2007; 178(5): 2651-4. PMID: 17312105, PII: 178/5/2651, ISSN: 0022-1767.
Tian C, Luskin GK, Dischert KM, Higginbotham JN, Shepherd BE, Crowe JE. Evidence for preferential Ig gene usage and differential TdT and exonuclease activities in human naïve and memory B cells. Mol. Immunol [print-electronic]. 2007 Mar; 44(9): 2173-83. PMID: 17196657, PMCID: PMC1859862, PII: S0161-5890(06)00701-2, DOI: 10.1016/j.molimm.2006.11.020, ISSN: 0161-5890.
Tian C, Kron GK, Dischert KM, Higginbotham JN, Crowe JE. Low expression of the interleukin (IL)-4 receptor alpha chain and reduced signalling via the IL-4 receptor complex in human neonatal B cells. Immunology [print-electronic]. 2006 Sep; 119(1): 54-62. PMID: 16764687, PMCID: PMC1782340, PII: IMM2405, DOI: 10.1111/j.1365-2567.2006.02405.x, ISSN: 0019-2805.
Choo EF, Kurnik D, Muszkat M, Ohkubo T, Shay SD, Higginbotham JN, Glaeser H, Kim RB, Wood AJ, Wilkinson GR. Differential in vivo sensitivity to inhibition of P-glycoprotein located in lymphocytes, testes, and the blood-brain barrier. J. Pharmacol. Exp. Ther [print-electronic]. 2006 Jun; 317(3): 1012-8. PMID: 16537797, PII: jpet.105.099648, DOI: 10.1124/jpet.105.099648, ISSN: 0022-3565.
Dou Y, Cha DJ, Franklin JL, Higginbotham JN, Jeppesen DK, Weaver AM, Prasad N, Levy S, Coffey RJ, Patton JG, Zhang B. Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes. Sci Rep. 2016 Nov 11/28/2016; 6: 37982. PMID: 27892494, PMCID: PMC5125100, PII: srep37982, DOI: 10.1038/srep37982, ISSN: 2045-2322.
Higginbotham JN, Zhang Q, Jeppesen DK, Scott AM, Manning HC, Ochieng J, Franklin JL, Coffey RJ. Identification and characterization of EGF receptor in individual exosomes by fluorescence-activated vesicle sorting. J Extracell Vesicles. 2016; 5: 29254. PMID: 27345057, PMCID: PMC4921784, PII: 29254, ISSN: 2001-3078.
Cha DJ, Franklin JL, Dou Y, Liu Q, Higginbotham JN, Demory Beckler M, Weaver AM, Vickers K, Prasad N, Levy S, Zhang B, Coffey RJ, Patton JG. KRAS-dependent sorting of miRNA to exosomes. Elife. 2015 Jul 7/1/2015; 4: e07197. PMID: 26132860, PMCID: PMC4510696, DOI: 10.7554/eLife.07197, ISSN: 2050-084X.
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Mol. Cell Proteomics [print-electronic]. 2013 Feb; 12(2): 343-55. PMID: 23161513, PMCID: PMC3567858, PII: M112.022806, DOI: 10.1074/mcp.M112.022806, ISSN: 1535-9484.
Shifrin DA, McConnell RE, Nambiar R, Higginbotham JN, Coffey RJ, Tyska MJ. Enterocyte microvillus-derived vesicles detoxify bacterial products and regulate epithelial-microbial interactions. Curr. Biol [print-electronic]. 2012 Apr 4/10/2012; 22(7): 627-31. PMID: 22386311, PMCID: PMC3326206, PII: S0960-9822(12)00171-6, DOI: 10.1016/j.cub.2012.02.022, ISSN: 1879-0445.
Powell AE, Wang Y, Li Y, Poulin EJ, Means AL, Washington MK, Higginbotham JN, Juchheim A, Prasad N, Levy SE, Guo Y, Shyr Y, Aronow BJ, Haigis KM, Franklin JL, Coffey RJ. The pan-ErbB negative regulator Lrig1 is an intestinal stem cell marker that functions as a tumor suppressor. Cell. 2012 Mar 3/30/2012; 149(1): 146-58. PMID: 22464327, PMCID: PMC3563328, PII: S0092-8674(12)00280-2, DOI: 10.1016/j.cell.2012.02.042, ISSN: 1097-4172.
Gephart JD, Singh B, Higginbotham JN, Franklin JL, Gonzalez A, Fölsch H, Coffey RJ. Identification of a novel mono-leucine basolateral sorting motif within the cytoplasmic domain of amphiregulin. Traffic [print-electronic]. 2011 Dec; 12(12): 1793-804. PMID: 21917092, PMCID: PMC3886124, DOI: 10.1111/j.1600-0854.2011.01282.x, ISSN: 1600-0854.
Higginbotham JN, Demory Beckler M, Gephart JD, Franklin JL, Bogatcheva G, Kremers GJ, Piston DW, Ayers GD, McConnell RE, Tyska MJ, Coffey RJ. Amphiregulin exosomes increase cancer cell invasion. Curr. Biol [print-electronic]. 2011 May 5/10/2011; 21(9): 779-86. PMID: 21514161, PMCID: PMC3417320, PII: S0960-9822(11)00347-2, DOI: 10.1016/j.cub.2011.03.043, ISSN: 1879-0445.
Lapierre LA, Caldwell CM, Higginbotham JN, Avant KM, Hall J, Beauchamp RD, Goldenring JR. Transformation of rat intestinal epithelial cells by overexpression of Rab25 is microtubule dependent. Cytoskeleton (Hoboken) [print-electronic]. 2011 Feb; 68(2): 97-111. PMID: 21246754, PMCID: PMC3885764, DOI: 10.1002/cm.20497, ISSN: 1949-3592.
McConnell RE, Higginbotham JN, Shifrin DA, Tabb DL, Coffey RJ, Tyska MJ. The enterocyte microvillus is a vesicle-generating organelle. J. Cell Biol. 2009 Jun 6/29/2009; 185(7): 1285-98. PMID: 19564407, PMCID: PMC2712962, PII: jcb.200902147, DOI: 10.1083/jcb.200902147, ISSN: 1540-8140.
Cao Z, Li C, Higginbotham JN, Franklin JL, Tabb DL, Graves-Deal R, Hill S, Cheek K, Jerome WG, Lapierre LA, Goldenring JR, Ham AJ, Coffey RJ. Use of fluorescence-activated vesicle sorting for isolation of Naked2-associated, basolaterally targeted exocytic vesicles for proteomics analysis. Mol. Cell Proteomics [print-electronic]. 2008 Sep; 7(9): 1651-67. PMID: 18504258, PMCID: PMC2528074, PII: M700155-MCP200, DOI: 10.1074/mcp.M700155-MCP200, ISSN: 1535-9484.
Tian C, Luskin GK, Dischert KM, Higginbotham JN, Shepherd BE, Crowe JE. Immunodominance of the VH1-46 antibody gene segment in the primary repertoire of human rotavirus-specific B cells is reduced in the memory compartment through somatic mutation of nondominant clones. J. Immunol. 2008 Mar 3/1/2008; 180(5): 3279-88. PMID: 18292552, PII: 180/5/3279, ISSN: 0022-1767.
Hartig SM, Greene RR, Carlesso G, Higginbotham JN, Khan WN, Prokop A, Davidson JM. Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells. Biomaterials [print-electronic]. 2007 Sep; 28(26): 3843-55. PMID: 17560645, PMCID: PMC2000344, PII: S0142-9612(07)00346-8, DOI: 10.1016/j.biomaterials.2007.04.027, ISSN: 0142-9612.
Moore ML, Chi MH, Zhou W, Goleniewska K, O'Neal JF, Higginbotham JN, Peebles RS. Cutting Edge: Oseltamivir decreases T cell GM1 expression and inhibits clearance of respiratory syncytial virus: potential role of endogenous sialidase in antiviral immunity. J. Immunol. 2007 Mar 3/1/2007; 178(5): 2651-4. PMID: 17312105, PII: 178/5/2651, ISSN: 0022-1767.
Tian C, Luskin GK, Dischert KM, Higginbotham JN, Shepherd BE, Crowe JE. Evidence for preferential Ig gene usage and differential TdT and exonuclease activities in human naïve and memory B cells. Mol. Immunol [print-electronic]. 2007 Mar; 44(9): 2173-83. PMID: 17196657, PMCID: PMC1859862, PII: S0161-5890(06)00701-2, DOI: 10.1016/j.molimm.2006.11.020, ISSN: 0161-5890.
Tian C, Kron GK, Dischert KM, Higginbotham JN, Crowe JE. Low expression of the interleukin (IL)-4 receptor alpha chain and reduced signalling via the IL-4 receptor complex in human neonatal B cells. Immunology [print-electronic]. 2006 Sep; 119(1): 54-62. PMID: 16764687, PMCID: PMC1782340, PII: IMM2405, DOI: 10.1111/j.1365-2567.2006.02405.x, ISSN: 0019-2805.
Choo EF, Kurnik D, Muszkat M, Ohkubo T, Shay SD, Higginbotham JN, Glaeser H, Kim RB, Wood AJ, Wilkinson GR. Differential in vivo sensitivity to inhibition of P-glycoprotein located in lymphocytes, testes, and the blood-brain barrier. J. Pharmacol. Exp. Ther [print-electronic]. 2006 Jun; 317(3): 1012-8. PMID: 16537797, PII: jpet.105.099648, DOI: 10.1124/jpet.105.099648, ISSN: 0022-3565.