F. Peter Guengerich, Ph.D.

638B RRB
TN 0146

Mechanisms of activation and detoxication of chemical carcinogens and toxicants; characterization of enzymes involved in these processes

Major areas of interest include the metabolism of carcinogens and drugs by cytochrome P450 enzymes, the bioactivation of halogenated hydrocarbons, and polymerase interactions with carcinogen-modified DNA. Several approaches are being used to understand the catalytic mechanisms of the human cytochrome P450 enzymes, including site-directed and random mutagenesis, kinetic analysis, isotope effects, and substrate-activity relationships. Work on halogenated hydrocarbons is done with trichloroethylene, ethylene dibromide, and methylene dihalides and the adducts they form. Glutathione transferase-mediated bioactivation is under investigation, as well as in the case of some bifunctional electrophiles. Studies on the polymerases involve the use of modified oligonucleotides, steady-state and pre-steady-state kinetics, and physical approaches such as x-ray diffraction. A major goal is to understand how carcinogen-DNA adducts cause polymerases to stall and misincorporate bases. See website at https://my.vanderbilt.edu/guengerichlab/.

N/A

Enzymology Cytochrome P450 DNA polymerases Steroid metabolism Drug metabolism Chemical carcinogenesis Enzyme kinetics Chemical synthesis Mass spectrometry Analytical biochemistry X-ray crystallography

Koinuma K, Noto K, Morita T, Uekusa Y, Kikuchi H, Shimoji M, Seki H, Yamazaki H, Guengerich FP, Nakamura K, Yamamoto K, Imaoka A, Akiyoshi T, Ohtani H. Kinetics of the inhibition of CYP3A4 and CYP2C19 activity by jabara juice and identification of the responsible inhibitory components. J Pharm Sci [print-electronic]. 2024 Oct 10/30/2024; PMID: 39481475, PII: S0022-3549(24)00487-8, DOI: 10.1016/j.xphs.2024.10.037, ISSN: 1520-6017.

Guengerich FP. Roles of Individual Human Cytochrome P450 Enzymes in Drug Metabolism. Pharmacol Rev. 2024 Oct 10/16/2024; 76(6): 1104-32. PMID: 39054072, PMCID: PMC11549934, PII: pharmrev.124.001173, DOI: 10.1124/pharmrev.124.001173, ISSN: 1521-0081.

Tomar R, Ghodke PP, Patra A, Smyth E, Pontarelli A, Copp W, Guengerich FP, Chaput JC, Wilds CJ, Stone MP, Egli M. DNA Replication across a-l-(3'-2')-Threofuranosyl Nucleotides Mediated by Human DNA Polymerase ¿. Biochemistry [print-electronic]. 2024 Oct 10/1/2024; 63(19): 2425-39. PMID: 39259676, PMCID: PMC11447838, DOI: 10.1021/acs.biochem.4c00387, ISSN: 1520-4995.

Guengerich FP, Tateishi Y, McCarty KD, Yoshimoto FK. Updates on Mechanisms of Cytochrome P450 Catalysis of Complex Steroid Oxidations. Int J Mol Sci. 2024 Aug 8/20/2024; 25(16): PMID: 39201706, PMCID: PMC11354347, PII: ijms25169020, DOI: 10.3390/ijms25169020, ISSN: 1422-0067.

Tateishi Y, McCarty KD, Martin MV, Yoshimoto FK, Guengerich FP. Roles of Ferric Peroxide Anion Intermediates (Fe³⁺O₂ ^-, Compound 0) in Cytochrome P450 19A1 Steroid Aromatization and a Cytochrome P450 2B4 Secosteroid Oxidation Model. Angew Chem Int Ed Engl [print-electronic]. 2024 Aug 8/12/2024; 63(33): e202406542. PMID: 38820076, PMCID: PMC11519728, DOI: 10.1002/anie.202406542, ISSN: 1521-3773.

Rendic SP, Guengerich FP. Formation of potentially toxic metabolites of drugs in reactions catalyzed by human drug-metabolizing enzymes. Arch Toxicol [print-electronic]. 2024 Jun; 98(6): 1581-628. PMID: 38520539, PMCID: PMC11539061, PII: 10.1007/s00204-024-03710-9, DOI: 10.1007/s00204-024-03710-9, ISSN: 1432-0738.

Uehara S, Murayama N, Higuchi Y, Shimizu M, Suemizu H, Guengerich FP, Yamazaki H. In Vivo and In Vitro Induction of Cytochrome P450 3A4 by Thalidomide in Humanized-Liver Mice and Experimental Human Hepatocyte HepaSH cells. Chem Res Toxicol [print-electronic]. 2024 May 5/20/2024; 37(5): 671-4. PMID: 38626399, PMCID: PMC11492454, DOI: 10.1021/acs.chemrestox.4c00069, ISSN: 1520-5010.

Guengerich FP. Cytochrome P450 Enzymes as Drug Targets in Human Disease. Drug Metab Dispos. 2024 May 5/16/2024; 52(6): 493-7. PMID: 37793784, PMCID: PMC11114603, PII: dmd.123.001431, DOI: 10.1124/dmd.123.001431, ISSN: 1521-009X.

Hargrove TY, Lamb DC, Wawrzak Z, Hull M, Kelly SL, Guengerich FP, Lepesheva GI. Identification of Potent and Selective Inhibitors of Acanthamoeba: Structural Insights into Sterol 14a-Demethylase as a Key Drug Target. J Med Chem [print-electronic]. 2024 May 5/9/2024; 67(9): 7443-57. PMID: 38683753, PMCID: PMC11089504, DOI: 10.1021/acs.jmedchem.4c00303, ISSN: 1520-4804.

Tateishi Y, Webb SN, Li B, Liu L, Lindsey Rose K, Leser M, Patel P, Guengerich FP. Proteomics, modeling, and fluorescence assays delineate cytochrome b₅ residues involved in binding and stimulation of cytochrome P450 17A1 17,20-lyase. J Biol Chem [print-electronic]. 2024 Mar; 300(3): 105688. PMID: 38280431, PMCID: PMC10878793, PII: S0021-9258(24)00064-4, DOI: 10.1016/j.jbc.2024.105688, ISSN: 1083-351X.

McCarty KD, Tateishi Y, Hargrove TY, Lepesheva GI, Guengerich FP. Oxygen-18 Labeling Reveals a Mixed Fe-O Mechanism in the Last Step of Cytochrome P450 51 Sterol 14a-Demethylation. Angew Chem Int Ed Engl [print-electronic]. 2024 Feb 2/26/2024; 63(9): e202317711. PMID: 38206808, PMCID: PMC11494732, DOI: 10.1002/anie.202317711, ISSN: 1521-3773.

Tateishi Y, McCarty KD, Martin MV, Guengerich FP. Oxygen-18 Labeling Defines a Ferric Peroxide (Compound 0) Mechanism in the Oxidative Deformylation of Aldehydes by Cytochrome P450 2B4. ACS Catal. 2024 Feb 2/16/2024; 14(4): 2388-94. PMID: 38384943, PMCID: PMC10877606, DOI: 10.1021/acscatal.4c00106, ISSN: 2155-5435.

Guengerich FP. Ninety-eight semesters of cytochrome P450 enzymes and related topics-What have I taught and learned?. J Biol Chem [print-electronic]. 2024 Feb; 300(2): 105625. PMID: 38185246, PMCID: PMC10847173, PII: S0021-9258(24)00001-2, DOI: 10.1016/j.jbc.2024.105625, ISSN: 1083-351X.

McCarty KD, Liu L, Tateishi Y, Wapshott-Stehli HL, Guengerich FP. The multistep oxidation of cholesterol to pregnenolone by human cytochrome P450 11A1 is highly processive. J Biol Chem [print-electronic]. 2024 Jan; 300(1): 105495. PMID: 38006947, PMCID: PMC10716780, PII: S0021-9258(23)02523-1, DOI: 10.1016/j.jbc.2023.105495, ISSN: 1083-351X.

Davidsen JM, Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Rosol TJ, Harman CL, Ramanan D, Taylor SV. In response to the Letter to the Editor from Xiao regarding our article, "FEMA GRAS assessment of natural flavor complexes: Asafetida oil, garlic oil and onion oil" [letter]. Food Chem Toxicol [print-electronic]. 2023 Dec; 182: 114040. PMID: 37726071, PII: S0278-6915(23)00442-8, DOI: 10.1016/j.fct.2023.114040, ISSN: 1873-6351.

Nagayoshi H, Murayama N, Kim V, Kim D, Takenaka S, Yamazaki H, Guengerich FP, Shimada T. Oxidation of Naringenin, Apigenin, and Genistein by Human Family 1 Cytochrome P450 Enzymes and Comparison of Interaction of Apigenin with Human P450 1B1.1 and Scutellaria P450 82D.1. Chem Res Toxicol [print-electronic]. 2023 Nov 11/20/2023; 36(11): 1778-88. PMID: 37783573, PMCID: PMC11497155, DOI: 10.1021/acs.chemrestox.3c00229, ISSN: 1520-5010.

Gooderham NJ, Cohen SM, Eisenbrand G, Fukushima S, Guengerich FP, Hecht SS, Rietjens IMCM, Rosol TJ, Davidsen JM, Harman CL, Kelly SE, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Sage oil, Orris Root Extract and Tagetes Oil and related flavoring ingredients. Food Chem Toxicol [print-electronic]. 2023 Sep; 179: 113940. PMID: 37487858, PII: S0278-6915(23)00342-3, DOI: 10.1016/j.fct.2023.113940, ISSN: 1873-6351.

Guengerich FP. Michael R. Waterman and Cytochrome P450. J Inorg Biochem [print-electronic]. 2023 Jul 7/26/2023; 247: 112328. PMID: 37506603, PII: S0162-0134(23)00210-6, DOI: 10.1016/j.jinorgbio.2023.112328, ISSN: 1873-3344.

Glass SM, Tateishi Y, Guengerich FP, Wang HJ. 3,4-Desaturation of retinoic acid by cytochrome P450 27C1 prevents P450-mediated catabolism. Arch Biochem Biophys [print-electronic]. 2023 Jul 7/15/2023; 743: 109669. PMID: 37356607, PMCID: PMC11500474, PII: S0003-9861(23)00168-6, DOI: 10.1016/j.abb.2023.109669, ISSN: 1096-0384.

McCarty KD, Sullivan ME, Tateishi Y, Hargrove TY, Lepesheva GI, Guengerich FP. Processive kinetics in the three-step lanosterol 14a-demethylation reaction catalyzed by human cytochrome P450 51A1. J Biol Chem [print-electronic]. 2023 Jul; 299(7): 104841. PMID: 37209823, PMCID: PMC10285260, PII: S0021-9258(23)01869-0, DOI: 10.1016/j.jbc.2023.104841, ISSN: 1083-351X.

Jian JY, McCarty KD, Byl JAW, Guengerich FP, Neuman KC, Osheroff N. Basis for the discrimination of supercoil handedness during DNA cleavage by human and bacterial type II topoisomerases. Nucleic Acids Res. 2023 May 5/8/2023; 51(8): 3888-902. PMID: 36999602, PMCID: PMC10164583, PII: 7097661, DOI: 10.1093/nar/gkad190, ISSN: 1362-4962.

Davidsen JM, Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Rosol TJ, Harman CL, Taylor SV. FEMA GRAS assessment of derivatives of basil, nutmeg, parsley, tarragon and related allylalkoxybenzene-containing natural flavor complexes. Food Chem Toxicol [print-electronic]. 2023 May; 175: 113646. PMID: 36804339, PII: S0278-6915(23)00048-0, DOI: 10.1016/j.fct.2023.113646, ISSN: 1873-6351.

Rosol TJ, Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Davidsen JM, Harman CL, Kelly S, Ramanan D, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Lemongrass oil, chamomile oils, citronella oil and related flavoring ingredients. Food Chem Toxicol [print-electronic]. 2023 May; 175: 113697. PMID: 36870670, PII: S0278-6915(23)00099-6, DOI: 10.1016/j.fct.2023.113697, ISSN: 1873-6351.

Kojima M, Machida K, Cho S, Watanabe D, Seki H, Shimoji M, Imaoka A, Yamazaki H, Guengerich FP, Nakamura K, Yamamoto K, Akiyoshi T, Ohtani H. The influence of temperature on the metabolic activity of CYP2C9, CYP2C19, and CYP3A4 genetic variants in vitro. Xenobiotica [print-electronic]. 2023 May; 53(5): 357-65. PMID: 37584614, PMCID: PMC11549676, DOI: 10.1080/00498254.2023.2248498, ISSN: 1366-5928.

Rietjens IMCM, Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rosol TJ, Davidsen JM, Harman CL, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Allspice, anise, fennel-derived and related flavoring ingredients. Food Chem Toxicol [print-electronic]. 2023 Apr; 174: 113643. PMID: 36739890, PII: S0278-6915(23)00045-5, DOI: 10.1016/j.fct.2023.113643, ISSN: 1873-6351.

Yeom M, Hong JK, Shin JH, Lee Y, Guengerich FP, Choi JY. Identification of Three Human POLH Germline Variants Defective in Complementing the UV- and Cisplatin-Sensitivity of POLH-Deficient Cells. Int J Mol Sci. 2023 Mar 3/8/2023; 24(6): PMID: 36982269, PMCID: PMC10048814, PII: ijms24065198, DOI: 10.3390/ijms24065198, ISSN: 1422-0067.

Davidsen JM, Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Rosol TJ, Harman CL, Ramanan D, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Asafetida oil, garlic oil and onion oil. Food Chem Toxicol [print-electronic]. 2023 Mar; 173: 113580. PMID: 36610475, PII: S0278-6915(22)00778-5, DOI: 10.1016/j.fct.2022.113580, ISSN: 1873-6351.

Lee SG, Kim V, Lee GH, Kim C, Jeong E, Guengerich FP, Kim D. Hydroxylation and lyase reactions of steroids catalyzed by mouse cytochrome P450 17A1 (Cyp17a1). J Inorg Biochem [print-electronic]. 2023 Mar; 240: 112085. PMID: 36640554, PMCID: PMC9892303, PII: S0162-0134(22)00374-9, DOI: 10.1016/j.jinorgbio.2022.112085, ISSN: 1873-3344.

Guengerich FP, McCarty KD, Tateishi Y, Liu L. Steroid 17a-hydroxylase/17, 20-lyase (cytochrome P450 17A1). Methods Enzymol [print-electronic]. 2023; 689: 39-63. PMID: 37802581, PMCID: PMC11512470, PII: S0076-6879(23)00125-8, DOI: 10.1016/bs.mie.2023.04.001, ISSN: 1557-7988.

Chakraborty S, Mallick D, Goswami M, Guengerich FP, Chakrabarty A, Chowdhury G. The Natural Products Withaferin A and Withanone from the Medicinal Herb Withania somnifera Are Covalent Inhibitors of the SARS-CoV-2 Main Protease. J Nat Prod [print-electronic]. 2022 Oct 10/28/2022; 85(10): 2340-50. PMID: 36098617, PMCID: PMC9491402, DOI: 10.1021/acs.jnatprod.2c00521, ISSN: 1520-6025.

Ghodke PP, Matse JH, Dawson S, Guengerich FP. Nucleophilic Thiol Proteins Bind Covalently to Abasic Sites in DNA. Chem Res Toxicol [print-electronic]. 2022 Oct 10/17/2022; 35(10): 1805-8. PMID: 35482010, DOI: 10.1021/acs.chemrestox.2c00068, ISSN: 1520-5010.

Guengerich FP. On 'Evidence for the participation of cytochrome b5 in hepatic microsomal mixed-function oxidation reactions' by Alfred Hildebrandt and Ronald W. Estabrook. Arch Biochem Biophys [print-electronic]. 2022 Sep 9/15/2022; 726: 109177. PMID: 35305998, PII: S0003-9861(22)00062-5, DOI: 10.1016/j.abb.2022.109177, ISSN: 1096-0384.

Rendic SP, Crouch RD, Guengerich FP. Roles of selected non-P450 human oxidoreductase enzymes in protective and toxic effects of chemicals: review and compilation of reactions. Arch Toxicol [print-electronic]. 2022 Aug; 96(8): 2145-246. PMID: 35648190, PMCID: PMC9159052, PII: 10.1007/s00204-022-03304-3, DOI: 10.1007/s00204-022-03304-3, ISSN: 1432-0738.

Guengerich FP. Drug metabolism: a half-century plus of progress, continued needs, and new opportunities. Drug Metab Dispos [print-electronic]. 2022 Jul 7/22/2022; PMID: 35868640, PII: dmd.121.000739, DOI: 10.1124/dmd.121.000739, ISSN: 1521-009X.

Shimada T, Nagayoshi H, Murayama N, Sawai A, Kim V, Kim D, Yamazaki H, Guengerich FP, Takenaka S. Oxidation of 3'-methoxyflavone, 4'-methoxyflavone, and 3',4'-dimethoxyflavone and their derivatives having 5,7-dihydroxyl moieties by human cytochromes P450 1B1 and 2A13. Xenobiotica [print-electronic]. 2022 Feb; 52(2): 134-45. PMID: 35387543, DOI: 10.1080/00498254.2022.2062486, ISSN: 1366-5928.

Guengerich FP. Inhibition of Cytochrome P450 Enzymes by Drugs-Molecular Basis and Practical Applications. Biomol Ther (Seoul). 2022 Jan 1/1/2022; 30(1): 1-18. PMID: 34475272, PMCID: PMC8724836, PII: biomolther.2021.102, DOI: 10.4062/biomolther.2021.102, ISSN: 1976-9148.

Guengerich FP. Roles of cytochrome P450 enzymes in pharmacology and toxicology: Past, present, and future. Adv Pharmacol [print-electronic]. 2022; 95: 1-47. PMID: 35953152, PII: S1054-3589(21)00066-1, DOI: 10.1016/bs.apha.2021.12.001, ISSN: 1557-8925.

Glass SM, Webb SN, Guengerich FP. Binding of cytochrome P450 27C1, a retinoid desaturase, to its accessory protein adrenodoxin. Arch Biochem Biophys [print-electronic]. 2021 Dec 12/15/2021; 714: 109076. PMID: 34732331, PMCID: PMC8629852, PII: S0003-9861(21)00325-8, DOI: 10.1016/j.abb.2021.109076, ISSN: 1096-0384.

Hargrove TY, Wawrzak Z, Rachakonda G, Nes WD, Villalta F, Guengerich FP, Lepesheva GI. Relaxed Substrate Requirements of Sterol 14a-Demethylase from Naegleria fowleri Are Accompanied by Resistance to Inhibition. J Med Chem [print-electronic]. 2021 Dec 12/9/2021; 64(23): 17511-22. PMID: 34842434, PMCID: PMC8667612, DOI: 10.1021/acs.jmedchem.1c01710, ISSN: 1520-4804.

Kim D, Kim V, Tateishi Y, Guengerich FP. Cytochrome b ₅ Binds Tightly to Several Human Cytochrome P450 Enzymes. Drug Metab Dispos [print-electronic]. 2021 Oct; 49(10): 902-9. PMID: 34330716, PMCID: PMC8626640, PII: dmd.121.000475, DOI: 10.1124/dmd.121.000475, ISSN: 1521-009X.

Ghodke PP, Guengerich FP. DNA polymerases ¿ and ¿ bypass N²-guanine-O⁶-alkylguanine DNA alkyltransferase cross-linked DNA-peptides. J Biol Chem [print-electronic]. 2021 Oct; 297(4): 101124. PMID: 34461101, PMCID: PMC8463853, PII: S0021-9258(21)00925-X, DOI: 10.1016/j.jbc.2021.101124, ISSN: 1083-351X.

Glass SM, Guengerich FP. Cellular retinoid-binding proteins transfer retinoids to human cytochrome P450 27C1 for desaturation. J Biol Chem [print-electronic]. 2021 Oct; 297(4): 101142. PMID: 34480899, PMCID: PMC8511960, PII: S0021-9258(21)00943-1, DOI: 10.1016/j.jbc.2021.101142, ISSN: 1083-351X.

Nagayoshi H, Murayama N, Takenaka S, Kim V, Kim D, Komori M, Yamazaki H, Guengerich FP, Shimada T. Roles of cytochrome P450 2A6 in the oxidation of flavone, 4'-hydroxyflavone, and 4'-, 3'-, and 2'-methoxyflavones by human liver microsomes. Xenobiotica [print-electronic]. 2021 Sep; 51(9): 995-1009. PMID: 34224301, PMCID: PMC8719450, DOI: 10.1080/00498254.2021.1950866, ISSN: 1366-5928.

Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Rosol TJ, Davidsen JM, Harman CL, Lu V, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Origanum oil, thyme oil and related phenol derivative-containing flavoring ingredients. Food Chem Toxicol [print-electronic]. 2021 Sep; 155: 112378. PMID: 34217738, PII: S0278-6915(21)00411-7, DOI: 10.1016/j.fct.2021.112378, ISSN: 1873-6351.

Eisenbrand G, Cohen SM, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Rosol TJ, Davidsen JM, Harman CL, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Eucalyptus oil and other cyclic ether-containing flavoring ingredients. Food Chem Toxicol [print-electronic]. 2021 Sep; 155: 112357. PMID: 34217737, PII: S0278-6915(21)00390-2, DOI: 10.1016/j.fct.2021.112357, ISSN: 1873-6351.

Guengerich FP, McCarty KD, Chapman JG, Tateishi Y. Stepwise binding of inhibitors to human cytochrome P450 17A1 and rapid kinetics of inhibition of androgen biosynthesis. J Biol Chem [print-electronic]. 2021 Aug; 297(2): 100969. PMID: 34273352, PMCID: PMC8350020, PII: S0021-9258(21)00770-5, DOI: 10.1016/j.jbc.2021.100969, ISSN: 1083-351X.

Guengerich FP, Ghodke PP. Etheno adducts: from tRNA modifications to DNA adducts and back to miscoding ribonucleotides. Genes Environ. 2021 Jun 6/16/2021; 43(1): 24. PMID: 34130743, PMCID: PMC8207595, PII: 10.1186/s41021-021-00199-x, DOI: 10.1186/s41021-021-00199-x, ISSN: 1880-7046.

Yamaguchi Y, Akiyoshi T, Kawamura G, Imaoka A, Miyazaki M, Guengerich FP, Nakamura K, Yamamoto K, Ohtani H. Comparison of the inhibitory effects of azole antifungals on cytochrome P450 3A4 genetic variants. Drug Metab Pharmacokinet [print-electronic]. 2021 Jun; 38: 100384. PMID: 33826998, PMCID: PMC8144059, PII: S1347-4367(21)00005-7, DOI: 10.1016/j.dmpk.2021.100384, ISSN: 1880-0920.

Glass SM, Reddish MJ, Child SA, Wilkey CJ, Stec DF, Guengerich FP. Characterization of human adrenal cytochrome P450 11B2 products of progesterone and androstenedione oxidation. J Steroid Biochem Mol Biol [print-electronic]. 2021 Apr; 208: 105787. PMID: 33189850, PMCID: PMC7954869, PII: S0960-0760(20)30312-5, DOI: 10.1016/j.jsbmb.2020.105787, ISSN: 1879-1220.

Shimada T, Nagayoshi H, Murayama N, Takenaka S, Katahira J, Kim V, Kim D, Komori M, Yamazaki H, Guengerich FP. Liquid chromatography-tandem mass spectrometry analysis of oxidation of 2'-, 3'-, 4'- and 6-hydroxyflavanones by human cytochrome P450 enzymes. Xenobiotica [print-electronic]. 2021 Feb; 51(2): 139-54. PMID: 33047997, PMCID: PMC7875482, DOI: 10.1080/00498254.2020.1836433, ISSN: 1366-5928.

Rendic SP, Guengerich FP. Human Family 1-4 cytochrome P450 enzymes involved in the metabolic activation of xenobiotic and physiological chemicals: an update. Arch Toxicol [print-electronic]. 2021 Feb; 95(2): 395-472. PMID: 33459808, PMCID: PMC7880545, PII: 10.1007/s00204-020-02971-4, DOI: 10.1007/s00204-020-02971-4, ISSN: 1432-0738.

Guengerich FP, Child SA, Barckhausen IR, Goldfarb MH. Kinetic Evidence for an Induced Fit Mechanism in the Binding of the Substrate Camphor by Cytochrome P450_cam. ACS Catal [print-electronic]. 2021 Jan 1/15/2021; 11(2): 639-49. PMID: 34327042, PMCID: PMC8318206, DOI: 10.1021/acscatal.0c04455, ISSN: 2155-5435.

Guengerich FP. A history of the roles of cytochrome P450 enzymes in the toxicity of drugs. Toxicol Res. 2021 Jan; 37(1): 1-23. PMID: 32837681, PMCID: PMC7431904, PII: 56, DOI: 10.1007/s43188-020-00056-z, ISSN: 1976-8257.

Guengerich FP, McCarty KD, Chapman JG. Kinetics of cytochrome P450 3A4 inhibition by heterocyclic drugs defines a general sequential multistep binding process. J Biol Chem [print-electronic]. 2021 Jan; 296: 100223. PMID: 33449875, PMCID: PMC7948456, PII: S0021-9258(20)00338-5, DOI: 10.1074/jbc.RA120.016855, ISSN: 1083-351X.

Ghodke PP, Gonzalez-Vasquez G, Wang H, Johnson KM, Sedgeman CA, Guengerich FP. Enzymatic bypass of an N⁶-deoxyadenosine DNA-ethylene dibromide-peptide cross-link by translesion DNA polymerases. J Biol Chem [print-electronic]. 2021 Jan; 296: 100444. PMID: 33617883, PMCID: PMC8024977, PII: S0021-9258(21)00217-9, DOI: 10.1016/j.jbc.2021.100444, ISSN: 1083-351X.

Kim D, Kim V, McCarty KD, Guengerich FP. Tight binding of cytochrome b₅ to cytochrome P450 17A1 is a critical feature of stimulation of C21 steroid lyase activity and androgen synthesis. J Biol Chem [print-electronic]. 2021 Jan; 296: 100571. PMID: 33753170, PMCID: PMC8080067, PII: S0021-9258(21)00349-5, DOI: 10.1016/j.jbc.2021.100571, ISSN: 1083-351X.

Ghodke PP, Mali JR, Patra A, Rizzo CJ, Guengerich FP, Egli M. Enzymatic bypass and the structural basis of miscoding opposite the DNA adduct 1,N²-ethenodeoxyguanosine by human DNA translesion polymerase ¿. J Biol Chem [print-electronic]. 2021 Jan; 296: 100642. PMID: 33839151, PMCID: PMC8121704, PII: S0021-9258(21)00428-2, DOI: 10.1016/j.jbc.2021.100642, ISSN: 1083-351X.

Child SA, Reddish MJ, Glass SM, Goldfarb MH, Barckhausen IR, Guengerich FP. Functional interactions of adrenodoxin with several human mitochondrial cytochrome P450 enzymes. Arch Biochem Biophys [print-electronic]. 2020 Nov 11/15/2020; 694: 108596. PMID: 32980349, PMCID: PMC7863566, PII: S0003-9861(20)30605-6, DOI: 10.1016/j.abb.2020.108596, ISSN: 1096-0384.

Gooderham NJ, Cohen SM, Eisenbrand G, Fukushima S, Guengerich FP, Hecht SS, Rietjens IMCM, Rosol TJ, Davidsen JM, Harman CL, Murray IJ, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Clove, cinnamon leaf and West Indian bay leaf-derived flavoring ingredients. Food Chem Toxicol [print-electronic]. 2020 Nov; 145: 111585. PMID: 32702506, PII: S0278-6915(20)30475-0, DOI: 10.1016/j.fct.2020.111585, ISSN: 1873-6351.

Fukushima S, Cohen SM, Eisenbrand G, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Rosol TJ, Davidsen JM, Harman CL, Lu V, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Lavender, Guaiac Coriander-derived and related flavoring ingredients. Food Chem Toxicol [print-electronic]. 2020 Nov; 145: 111584. PMID: 32682832, PII: S0278-6915(20)30474-9, DOI: 10.1016/j.fct.2020.111584, ISSN: 1873-6351.

Ahmed N, Chakrabarty A, Guengerich FP, Chowdhury G. Protective Role of Glutathione against Peroxynitrite-Mediated DNA Damage During Acute Inflammation. Chem Res Toxicol [print-electronic]. 2020 Oct 10/19/2020; 33(10): 2668-74. PMID: 32894672, PMCID: PMC9297005, DOI: 10.1021/acs.chemrestox.0c00299, ISSN: 1520-5010.

Nagayoshi H, Murayama N, Tsujino M, Takenaka S, Katahira J, Kim V, Kim D, Komori M, Yamazaki H, Guengerich FP, Shimada T. Preference for O-demethylation reactions in the oxidation of 2'-, 3'-, and 4'-methoxyflavones by human cytochrome P450 enzymes. Xenobiotica [print-electronic]. 2020 Oct; 50(10): 1158-69. PMID: 32312164, PMCID: PMC7881365, DOI: 10.1080/00498254.2020.1759157, ISSN: 1366-5928.

Yeom M, Hong JK, Kim JK, Guengerich FP, Choi JY. Three Human Pol ¿ Variants with Impaired Polymerase Activity Fail to Rescue H₂O₂ Sensitivity in POLI-Deficient Cells. Chem Res Toxicol [print-electronic]. 2020 Aug 8/17/2020; 33(8): 2120-9. PMID: 32635723, PMCID: PMC9284323, DOI: 10.1021/acs.chemrestox.0c00127, ISSN: 1520-5010.

Hargrove TY, Wawrzak Z, Guengerich FP, Lepesheva GI. A requirement for an active proton delivery network supports a compound I-mediated C-C bond cleavage in CYP51 catalysis. J Biol Chem [print-electronic]. 2020 Jul 7/17/2020; 295(29): 9998-10007. PMID: 32493730, PMCID: PMC7380200, PII: S0021-9258(17)48939-3, DOI: 10.1074/jbc.RA120.014064, ISSN: 1083-351X.

Mast N, Verwilst P, Wilkey CJ, Guengerich FP, Pikuleva IA. In Vitro Activation of Cytochrome P450 46A1 (CYP46A1) by Efavirenz-Related Compounds. J Med Chem [print-electronic]. 2020 Jun 6/25/2020; 63(12): 6477-88. PMID: 31617715, PMCID: PMC7226586, DOI: 10.1021/acs.jmedchem.9b01383, ISSN: 1520-4804.

Child SA, Guengerich FP. Multistep Binding of the Non-Steroidal Inhibitors Orteronel and Seviteronel to Human Cytochrome P450 17A1 and Relevance to Inhibition of Enzyme Activity. J Med Chem [print-electronic]. 2020 Jun 6/25/2020; 63(12): 6513-22. PMID: 32223238, PMCID: PMC7875674, DOI: 10.1021/acs.jmedchem.9b01849, ISSN: 1520-4804.

Ghodke PP, Guengerich FP. Impact of 1,N ⁶-ethenoadenosine, a damaged ribonucleotide in DNA, on translesion synthesis and repair. J Biol Chem [print-electronic]. 2020 May 5/1/2020; 295(18): 6092-107. PMID: 32213600, PMCID: PMC7196658, PII: S0021-9258(17)48238-X, DOI: 10.1074/jbc.RA120.012829, ISSN: 1083-351X.

Guengerich FP. Cytochrome P450 2E1 and its roles in disease. Chem Biol Interact [print-electronic]. 2020 May 5/1/2020; 322: 109056. PMID: 32198084, PMCID: PMC7217708, PII: S0009-2797(19)31126-3, DOI: 10.1016/j.cbi.2020.109056, ISSN: 1872-7786.

Guengerich FP, Fekry MI. Methylene Oxidation of Alkyl Sulfates by Cytochrome P450_BM-3 and a Role for Conformational Selection in Substrate Recognition. ACS Catal [print-electronic]. 2020 May 5/1/2020; 10(9): 5008-22. PMID: 34327041, PMCID: PMC8317952, DOI: 10.1021/acscatal.0c00677, ISSN: 2155-5435.

Li Z, Jiang Y, Guengerich FP, Ma L, Li S, Zhang W. Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications. J Biol Chem [print-electronic]. 2020 Jan 1/17/2020; 295(3): 833-49. PMID: 31811088, PMCID: PMC6970918, PII: S0021-9258(17)49939-X, DOI: 10.1074/jbc.REV119.008758, ISSN: 1083-351X.

Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Bastaki M, Davidsen JM, Harman CL, McGowen MM, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Mint, buchu, dill and caraway derived flavoring ingredients. Food Chem Toxicol [print-electronic]. 2020 Jan; 135: 110870. PMID: 31604112, PII: S0278-6915(19)30660-X, DOI: 10.1016/j.fct.2019.110870, ISSN: 1873-6351.

Gooderham NJ, Cohen SM, Eisenbrand G, Fukushima S, Guengerich FP, Hecht SS, Rietjens IMCM, Rosol TJ, Bastaki M, Linman MJ, Taylor SV. The safety evaluation of food flavoring substances: the role of genotoxicity studies. Crit Rev Toxicol [print-electronic]. 2020 Jan; 50(1): 1-27. PMID: 32162576, DOI: 10.1080/10408444.2020.1712589, ISSN: 1547-6898.

Rietjens IMCM, Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rosol TJ, Davidsen JM, Harman CL, Murray IJ, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Cinnamomum and Myroxylon-derived flavoring ingredients. Food Chem Toxicol [print-electronic]. 2020 Jan; 135: 110949. PMID: 31751643, PII: S0278-6915(19)30739-2, DOI: 10.1016/j.fct.2019.110949, ISSN: 1873-6351.

Rendic S, Guengerich FP. Metabolism and Interactions of Chloroquine and Hydroxychloroquine with Human Cytochrome P450 Enzymes and Drug Transporters. Curr Drug Metab. 2020; 21(14): 1127-35. PMID: 33292107, PMCID: PMC8717092, PII: CDM-EPUB-112198, DOI: 10.2174/1389200221999201208211537, ISSN: 1875-5453.

Friggeri L, Hargrove TY, Wawrzak Z, Guengerich FP, Lepesheva GI. Validation of Human Sterol 14a-Demethylase (CYP51) Druggability: Structure-Guided Design, Synthesis, and Evaluation of Stoichiometric, Functionally Irreversible Inhibitors. J Med Chem [print-electronic]. 2019 Nov 11/27/2019; 62(22): 10391-401. PMID: 31663733, PMCID: PMC6881533, DOI: 10.1021/acs.jmedchem.9b01485, ISSN: 1520-4804.

Fekry MI, Xiao Y, Berg JZ, Guengerich FP. A Role for the Orphan Human Cytochrome P450 2S1 in Polyunsaturated Fatty Acid ¿-1 Hydroxylation Using an Untargeted Metabolomic Approach. Drug Metab Dispos [print-electronic]. 2019 Nov; 47(11): 1325-32. PMID: 31511258, PMCID: PMC6800448, PII: dmd.119.089086, DOI: 10.1124/dmd.119.089086, ISSN: 1521-009X.

Guengerich FP. Kinetic Modeling of Steady-State Situations in Cytochrome P450 Enzyme Reactions. Drug Metab Dispos [print-electronic]. 2019 Nov; 47(11): 1232-9. PMID: 31427434, PMCID: PMC6815944, PII: dmd.119.088732, DOI: 10.1124/dmd.119.088732, ISSN: 1521-009X.

Reddish MJ, Guengerich FP. Human cytochrome P450 11B2 produces aldosterone by a processive mechanism due to the lactol form of the intermediate 18-hydroxycorticosterone. J Biol Chem [print-electronic]. 2019 Aug 8/30/2019; 294(35): 12975-91. PMID: 31296661, PMCID: PMC6721951, PII: S0021-9258(20)43234-X, DOI: 10.1074/jbc.RA119.009830, ISSN: 1083-351X.

Nagayoshi H, Murayama N, Kakimoto K, Takenaka S, Katahira J, Lim YR, Kim V, Kim D, Yamazaki H, Komori M, Guengerich FP, Shimada T. Site-specific oxidation of flavanone and flavone by cytochrome P450 2A6 in human liver microsomes. Xenobiotica [print-electronic]. 2019 Jul; 49(7): 791-802. PMID: 30048196, PMCID: PMC6438780, DOI: 10.1080/00498254.2018.1505064, ISSN: 1366-5928.

Guengerich FP, Wilkey CJ, Phan TTN. Human cytochrome P450 enzymes bind drugs and other substrates mainly through conformational-selection modes. J. Biol. Chem [print-electronic]. 2019 May 5/30/2019; PMID: 31147443, PII: RA119.009305, DOI: 10.1074/jbc.RA119.009305, ISSN: 1083-351X.

Guengerich FP, Wilkey CJ, Phan TTN. Human cytochrome P450 enzymes bind drugs and other substrates mainly through conformational-selection modes. J. Biol. Chem [print-electronic]. 2019 May 5/30/2019; PMID: 31147443, PII: RA119.009305, DOI: 10.1074/jbc.RA119.009305, ISSN: 1083-351X.

Chattopadhyay M, Roy Chowdhury A, Feng T, Assenmacher CA, Radaelli E, Guengerich FP, Avadhani N. Mitochondria-targeted cytochrome P450 CYP2D6 is involved in monomethylamine-induced neuronal damage in mouse models. J. Biol. Chem [print-electronic]. 2019 May 5/20/2019; PMID: 31113867, PII: RA119.008848, DOI: 10.1074/jbc.RA119.008848, ISSN: 1083-351X.

Chattopadhyay M, Roy Chowdhury A, Feng T, Assenmacher CA, Radaelli E, Guengerich FP, Avadhani N. Mitochondria-targeted cytochrome P450 CYP2D6 is involved in monomethylamine-induced neuronal damage in mouse models. J. Biol. Chem [print-electronic]. 2019 May 5/20/2019; PMID: 31113867, PII: RA119.008848, DOI: 10.1074/jbc.RA119.008848, ISSN: 1083-351X.

Njuma OJ, Su Y, Guengerich FP. The abundant DNA adduct N7-methyl deoxyguanosine contributes to miscoding during replication by human DNA polymerase ¿. J. Biol. Chem [print-electronic]. 2019 May 5/17/2019; PMID: 31101656, PII: RA119.008986, DOI: 10.1074/jbc.RA119.008986, ISSN: 1083-351X.

Njuma OJ, Su Y, Guengerich FP. The abundant DNA adduct N7-methyl deoxyguanosine contributes to miscoding during replication by human DNA polymerase ¿. J. Biol. Chem [print-electronic]. 2019 May 5/17/2019; PMID: 31101656, PII: RA119.008986, DOI: 10.1074/jbc.RA119.008986, ISSN: 1083-351X.

Guengerich FP, Wilkey CJ, Glass SM, Reddish MJ. Conformational selection dominates binding of steroids to human cytochrome P450 17A1. J. Biol. Chem [print-electronic]. 2019 May 5/9/2019; PMID: 31072872, PII: RA119.008860, DOI: 10.1074/jbc.RA119.008860, ISSN: 1083-351X.

Guengerich FP, Wilkey CJ, Glass SM, Reddish MJ. Conformational selection dominates binding of steroids to human cytochrome P450 17A1. J. Biol. Chem [print-electronic]. 2019 May 5/9/2019; PMID: 31072872, PII: RA119.008860, DOI: 10.1074/jbc.RA119.008860, ISSN: 1083-351X.

Guengerich FP. Judy L. Bolton, 1962-2019. Chem. Res. Toxicol [print-electronic]. 2019 Apr 4/30/2019; PMID: 31038927, DOI: 10.1021/acs.chemrestox.9b00157, ISSN: 1520-5010.

Guengerich FP. Judy L. Bolton, 1962-2019. Chem. Res. Toxicol [print-electronic]. 2019 Apr 4/30/2019; PMID: 31038927, DOI: 10.1021/acs.chemrestox.9b00157, ISSN: 1520-5010.

Albertolle ME, Glass SM, Trefts E, Guengerich FP. Isotopic tagging of oxidized and reduced cysteines (iTORC) for detecting and quantifying sulfenic acids, disulfides, and free thiols in cells. J. Biol. Chem [print-electronic]. 2019 Apr 4/19/2019; 294(16): 6522-30. PMID: 30850396, PMCID: PMC6484142, PII: AC118.007225, DOI: 10.1074/jbc.AC118.007225, ISSN: 1083-351X.

Nagayoshi H, Murayama N, Kakimoto K, Tsujino M, Takenaka S, Katahira J, Lim YR, Kim D, Yamazaki H, Komori M, Guengerich FP, Shimada T. Oxidation of Flavone, 5-Hydroxyflavone, and 5,7-Dihydroxyflavone to Mono-, Di-, and Tri-Hydroxyflavones by Human Cytochrome P450 Enzymes. Chem. Res. Toxicol [print-electronic]. 2019 Apr 4/17/2019; PMID: 30964977, DOI: 10.1021/acs.chemrestox.9b00078, ISSN: 1520-5010.

Nagayoshi H, Murayama N, Kakimoto K, Tsujino M, Takenaka S, Katahira J, Lim YR, Kim D, Yamazaki H, Komori M, Guengerich FP, Shimada T. Oxidation of Flavone, 5-Hydroxyflavone, and 5,7-Dihydroxyflavone to Mono-, Di-, and Tri-Hydroxyflavones by Human Cytochrome P450 Enzymes. Chem. Res. Toxicol [print-electronic]. 2019 Apr 4/17/2019; PMID: 30964977, DOI: 10.1021/acs.chemrestox.9b00078, ISSN: 1520-5010.

Su Y, Ghodke PP, Egli M, Li L, Wang Y, Guengerich FP. Human DNA polymerase ¿ has reverse transcriptase activity in cellular environments. J. Biol. Chem [print-electronic]. 2019 Apr 4/12/2019; 294(15): 6073-81. PMID: 30842261, PMCID: PMC6463694, PII: RA119.007925, DOI: 10.1074/jbc.RA119.007925, ISSN: 1083-351X.

Albertolle ME, Song HD, Wilkey CJ, Segrest JP, Guengerich FP. Glutamine-451 Confers Sensitivity to Oxidative Inhibition and Heme-Thiolate Sulfenylation of Cytochrome P450 4B1. Chem. Res. Toxicol [print-electronic]. 2019 Mar 3/18/2019; 32(3): 484-92. PMID: 30701961, DOI: 10.1021/acs.chemrestox.8b00353, ISSN: 1520-5010.

Ghodke PP, Albertolle ME, Johnson KM, Guengerich FP. Synthesis and Characterization of Site-Specific O⁶ -Alkylguanine DNA-Alkyl Transferase-Oligonucleotide Crosslinks. Curr Protoc Nucleic Acid Chem [print-electronic]. 2019 Mar; 76(1): e74. PMID: 30657645, PMCID: PMC6504252, DOI: 10.1002/cpnc.74, ISSN: 1934-9289.

Guengerich FP, Georg GI, Wang S. Drug Metabolism and Toxicology Special Issue Call for Papers. J. Med. Chem [print-electronic]. 2019 Feb 2/14/2019; 62(3): 1077. PMID: 30990043, DOI: 10.1021/acs.jmedchem.9b00005, ISSN: 1520-4804.

Guengerich FP, Georg GI, Wang S. Drug Metabolism and Toxicology Special Issue Call for Papers. J. Med. Chem [print-electronic]. 2019 Feb 2/14/2019; 62(3): 1077. PMID: 30990043, DOI: 10.1021/acs.jmedchem.9b00005, ISSN: 1520-4804.

Kakimoto K, Murayama N, Takenaka S, Nagayoshi H, Lim YR, Kim V, Kim D, Yamazaki H, Komori M, Guengerich FP, Shimada T. Cytochrome P450 2A6 and other human P450 enzymes in the oxidation of flavone and flavanone. Xenobiotica [print-electronic]. 2019 Feb; 49(2): 131-42. PMID: 29310511, PMCID: PMC6430112, DOI: 10.1080/00498254.2018.1426133, ISSN: 1366-5928.

Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Bastaki M, Davidsen JM, Harman CL, McGowen M, Taylor SV. FEMA GRAS assessment of natural flavor complexes: Citrus-derived flavoring ingredients. Food Chem. Toxicol [print-electronic]. 2019 Feb; 124: 192-218. PMID: 30481573, PII: S0278-6915(18)30854-8, DOI: 10.1016/j.fct.2018.11.052, ISSN: 1873-6351.

Gierasch LM, Guengerich FP. Celebrating the scientific legacy of Herbert Tabor. J. Biol. Chem. 2019 Feb 2/1/2019; 294(5): 1635-7. PMID: 30710010, PMCID: PMC6364778, PII: 294/5/1635, DOI: 10.1074/jbc.TME119.007427, ISSN: 1083-351X.

Hargrove TY, Wawrzak Z, Fisher PM, Child SA, Nes WD, Guengerich FP, Waterman MR, Lepesheva GI. Binding of a physiological substrate causes large-scale conformational reorganization in cytochrome P450 51. J. Biol. Chem [print-electronic]. 2018 Dec 12/14/2018; 293(50): 19344-53. PMID: 30327430, PMCID: PMC6302162, PII: RA118.005850, DOI: 10.1074/jbc.RA118.005850, ISSN: 1083-351X.

Friggeri L, Hargrove TY, Rachakonda G, Blobaum AL, Fisher P, de Oliveira GM, da Silva CF, Soeiro MNC, Nes WD, Lindsley CW, Villalta F, Guengerich FP, Lepesheva GI. Sterol 14a-Demethylase Structure-Based Optimization of Drug Candidates for Human Infections with the Protozoan Trypanosomatidae. J. Med. Chem [print-electronic]. 2018 Dec 12/13/2018; 61(23): 10910-21. PMID: 30451500, PMCID: PMC6467724, DOI: 10.1021/acs.jmedchem.8b01671, ISSN: 1520-4804.

Guengerich FP. Mechanisms of Cytochrome P450-Catalyzed Oxidations. ACS Catal [print-electronic]. 2018 Dec 12/7/2018; 8(12): 10964-76. PMID: 31105987, PMCID: PMC6519473, DOI: 10.1021/acscatal.8b03401, ISSN: 2155-5435.

Guengerich FP. Mechanisms of Cytochrome P450-Catalyzed Oxidations. ACS Catal [print-electronic]. 2018 Dec 12/7/2018; 8(12): 10964-76. PMID: 31105987, PMCID: PMC6519473, DOI: 10.1021/acscatal.8b03401, ISSN: 2155-5435.

Sausville LN, Gangadhariah MH, Chiusa M, Mei S, Wei S, Zent R, Luther JM, Shuey MM, Capdevila JH, Falck JR, Guengerich FP, Williams SM, Pozzi A. The Cytochrome P450 Slow Metabolizers CYP2C9*2 and CYP2C9*3 Directly Regulate Tumorigenesis via Reduced Epoxyeicosatrienoic Acid Production. Cancer Res [print-electronic]. 2018 Sep 9/1/2018; 78(17): 4865-77. PMID: 30012669, PMCID: PMC6125168, PII: 0008-5472.CAN-17-3977, DOI: 10.1158/0008-5472.CAN-17-3977, ISSN: 1538-7445.

Guengerich FP, Yoshimoto FK. Formation and Cleavage of C-C Bonds by Enzymatic Oxidation-Reduction Reactions. Chem. Rev [print-electronic]. 2018 Jul 7/25/2018; 118(14): 6573-655. PMID: 29932643, PMCID: PMC6339258, DOI: 10.1021/acs.chemrev.8b00031, ISSN: 1520-6890.

Friggeri L, Hargrove TY, Wawrzak Z, Blobaum AL, Rachakonda G, Lindsley CW, Villalta F, Nes WD, Botta M, Guengerich FP, Lepesheva GI. Sterol 14a-Demethylase Structure-Based Design of VNI (( R)- N-(1-(2,4-Dichlorophenyl)-2-(1 H-imidazol-1-yl)ethyl)-4-(5-phenyl-1,3,4-oxadiazol-2-yl)benzamide)) Derivatives To Target Fungal Infections: Synthesis, Biological Evaluation, and Crystallographic Analysis. J. Med. Chem [print-electronic]. 2018 Jul 7/12/2018; 61(13): 5679-91. PMID: 29894182, PMCID: PMC6176729, DOI: 10.1021/acs.jmedchem.8b00641, ISSN: 1520-4804.

Smith RL, Cohen SM, Fukushima S, Gooderham NJ, Hecht SS, Guengerich FP, Rietjens IMCM, Bastaki M, Harman CL, McGowen MM, Taylor SV. The safety evaluation of food flavouring substances: the role of metabolic studies. Toxicol Res (Camb). 2018 Jul 7/1/2018; 7(4): 618-46. PMID: 30090611, PMCID: PMC6062396, PII: c7tx00254h, DOI: 10.1039/c7tx00254h, ISSN: 2045-452X.

Guengerich FP. Cytochrome P450 research and The Journal of Biological Chemistry. J. Biol. Chem [print-electronic]. 2018 Jun 6/5/2018; PMID: 29871932, PMCID: PMC6364786, PII: TM118.004144, DOI: 10.1074/jbc.TM118.004144, ISSN: 1083-351X.

Albertolle ME, Phan TTN, Pozzi A, Guengerich FP. Sulfenylation of Human Liver and Kidney Microsomal Cytochromes P450 and Other Drug-Metabolizing Enzymes as a Response to Redox Alteration. Mol. Cell Proteomics [print-electronic]. 2018 May; 17(5): 889-900. PMID: 29374135, PMCID: PMC5930400, PII: RA117.000382, DOI: 10.1074/mcp.RA117.000382, ISSN: 1535-9484.

Guengerich FP. Introduction to Metals in Biology 2018: Copper homeostasis and utilization in redox enzymes. J. Biol. Chem [print-electronic]. 2018 Mar 3/30/2018; 293(13): 4603-5. PMID: 29425098, PMCID: PMC5880126, PII: TM118.002255, DOI: 10.1074/jbc.TM118.002255, ISSN: 1083-351X.

Cohen SM, Eisenbrand G, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IMCM, Davidsen JM, Harman CL, Taylor SV. Updated procedure for the safety evaluation of natural flavor complexes used as ingredients in food. Food Chem. Toxicol [print-electronic]. 2018 Mar; 113: 171-8. PMID: 29355624, PII: S0278-6915(18)30021-8, DOI: 10.1016/j.fct.2018.01.021, ISSN: 1873-6351.

Soucek P, Vrana D, Ueng YF, Wei S, Kozevnikovova R, Guengerich FP. Selective changes in cholesterol metabolite levels in plasma of breast cancer patients after tumor removal [letter]. Clin. Chem. Lab. Med. 2018 Feb 2/23/2018; 56(3): e78-e81. PMID: 28915107, PMCID: PMC5792305, PII: /j/cclm.ahead-of-print/cclm-2017-0409/cclm-2017-0409.xml, DOI: 10.1515/cclm-2017-0409, ISSN: 1437-4331.

Gonzalez E, Johnson KM, Pallan PS, Phan TTN, Zhang W, Lei L, Wawrzak Z, Yoshimoto FK, Egli M, Guengerich FP. Inherent steroid 17a,20-lyase activity in defunct cytochrome P450 17A enzymes. J. Biol. Chem [print-electronic]. 2018 Jan 1/12/2018; 293(2): 541-56. PMID: 29212707, PMCID: PMC5767860, PII: RA117.000504, DOI: 10.1074/jbc.RA117.000504, ISSN: 1083-351X.

Rendic SP, Guengerich FP. Development and Uses of Offline and Web-Searchable Metabolism Databases - The Case of Benzo[a]pyrene. Curr. Drug Metab. 2018; 19(1): 3-46. PMID: 29219051, PMCID: PMC5886801, PII: CDM-EPUB-87298, DOI: 10.2174/1389200219666171207123939, ISSN: 1875-5453.

Murayama N, Suemizu H, Uehara S, Kusama T, Mitsui M, Kamiya Y, Shimizu M, Guengerich FP, Yamazaki H. Association of pharmacokinetic profiles of lenalidomide in human plasma simulated using pharmacokinetic data in humanized-liver mice with liver toxicity detected by human serum albumin RNA. J Toxicol Sci. 2018; 43(6): 369-75. PMID: 29877213, PMCID: PMC6348384, DOI: 10.2131/jts.43.369, ISSN: 1880-3989.

Su Y, Egli M, Guengerich FP. Human DNA polymerase ¿ accommodates RNA for strand extension. J. Biol. Chem [print-electronic]. 2017 Nov 11/3/2017; 292(44): 18044-51. PMID: 28972162, PMCID: PMC5672030, PII: M117.809723, DOI: 10.1074/jbc.M117.809723, ISSN: 1083-351X.

Shimizu M, Suemizu H, Mitsui M, Shibata N, Guengerich FP, Yamazaki H. Metabolic profiles of pomalidomide in human plasma simulated with pharmacokinetic data in control and humanized-liver mice. Xenobiotica [print-electronic]. 2017 Oct; 47(10): 844-8. PMID: 27852146, PMCID: PMC5433912, DOI: 10.1080/00498254.2016.1247218, ISSN: 1366-5928.

Wang CW, Huang CC, Chou PH, Chang YP, Wei S, Guengerich FP, Chou YC, Wang SF, Lai PS, Soucek P, Ueng YF. 7-ketocholesterol and 27-hydroxycholesterol decreased doxorubicin sensitivity in breast cancer cells: estrogenic activity and mTOR pathway. Oncotarget. 2017 Sep 9/12/2017; 8(39): 66033-50. PMID: 29029490, PMCID: PMC5630390, PII: 19789, DOI: 10.18632/oncotarget.19789, ISSN: 1949-2553.

Johnson KM, Phan TTN, Albertolle ME, Guengerich FP. Human mitochondrial cytochrome P450 27C1 is localized in skin and preferentially desaturates trans-retinol to 3,4-dehydroretinol. J. Biol. Chem [print-electronic]. 2017 Aug 8/18/2017; 292(33): 13672-87. PMID: 28701464, PII: M116.773937, DOI: 10.1074/jbc.M116.773937, ISSN: 1083-351X.

Gonzalez E, Guengerich FP. Kinetic processivity of the two-step oxidations of progesterone and pregnenolone to androgens by human cytochrome P450 17A1. J. Biol. Chem [print-electronic]. 2017 Aug 8/11/2017; 292(32): 13168-85. PMID: 28684414, PMCID: PMC5555180, PII: M117.794917, DOI: 10.1074/jbc.M117.794917, ISSN: 1083-351X.

Guengerich FP. Introduction to Metals in Biology 2017: Iron transport, storage, and the ramifications. J. Biol. Chem [print-electronic]. 2017 Aug 8/4/2017; 292(31): 12725-6. PMID: 28615455, PMCID: PMC5546012, PII: R117.801332, DOI: 10.1074/jbc.R117.801332, ISSN: 1083-351X.

Mast N, Anderson KW, Johnson KM, Phan TTN, Guengerich FP, Pikuleva IA. In vitro cytochrome P450 46A1 (CYP46A1) activation by neuroactive compounds. J. Biol. Chem [print-electronic]. 2017 Aug 8/4/2017; 292(31): 12934-46. PMID: 28642370, PMCID: PMC5546033, PII: M117.794909, DOI: 10.1074/jbc.M117.794909, ISSN: 1083-351X.

Wani TH, Chakrabarty A, Shibata N, Yamazaki H, Guengerich FP, Chowdhury G. The Dihydroxy Metabolite of the Teratogen Thalidomide Causes Oxidative DNA Damage. Chem. Res. Toxicol [print-electronic]. 2017 Aug 8/2/2017; PMID: 28745489, DOI: 10.1021/acs.chemrestox.7b00127, ISSN: 1520-5010.

Shimada T, Murayama N, Kakimoto K, Takenaka S, Lim YR, Yeom S, Kim D, Yamazaki H, Guengerich FP, Komori M. Oxidation of 1-chloropyrene by human CYP1 family and CYP2A subfamily cytochrome P450 enzymes: catalytic roles of two CYP1B1 and five CYP2A13 allelic variants. Xenobiotica [print-electronic]. 2017 Jul 7/21/2017; 1-11. PMID: 28648140, DOI: 10.1080/00498254.2017.1347306, ISSN: 1366-5928.

Albertolle ME, Kim D, Nagy LD, Yun CH, Pozzi A, Savas Ü, Johnson EF, Guengerich FP. Heme-thiolate sulfenylation of human cytochrome P450 4A11 functions as a redox switch for catalytic inhibition. J. Biol. Chem [print-electronic]. 2017 Jul 7/7/2017; 292(27): 11230-42. PMID: 28533430, PMCID: PMC5500791, PII: M117.792200, DOI: 10.1074/jbc.M117.792200, ISSN: 1083-351X.

Kloudova A, Guengerich FP, Soucek P. The Role of Oxysterols in Human Cancer. Trends Endocrinol. Metab [print-electronic]. 2017 Jul; 28(7): 485-96. PMID: 28410994, PMCID: PMC5474130, PII: S1043-2760(17)30038-3, DOI: 10.1016/j.tem.2017.03.002, ISSN: 1879-3061.

Wang C, Pallan PS, Zhang W, Lei L, Yoshimoto FK, Waterman MR, Egli M, Guengerich FP. Functional analysis of human cytochrome P450 21A2 variants involved in congenital adrenal hyperplasia. J. Biol. Chem [print-electronic]. 2017 Jun 6/30/2017; 292(26): 10767-78. PMID: 28539365, PMCID: PMC5491764, PII: M117.792465, DOI: 10.1074/jbc.M117.792465, ISSN: 1083-351X.

Sedgeman CA, Su Y, Guengerich FP. Formation of S-[2-(N(6)-Deoxyadenosinyl)ethyl]glutathione in DNA and Replication Past the Adduct by Translesion DNA Polymerases. Chem. Res. Toxicol [print-electronic]. 2017 May 5/15/2017; 30(5): 1188-96. PMID: 28395138, DOI: 10.1021/acs.chemrestox.7b00022, ISSN: 1520-5010.

Hargrove TY, Friggeri L, Wawrzak Z, Qi A, Hoekstra WJ, Schotzinger RJ, York JD, Guengerich FP, Lepesheva GI. Structural analyses of Candida albicans sterol 14a-demethylase complexed with azole drugs address the molecular basis of azole-mediated inhibition of fungal sterol biosynthesis. J. Biol. Chem [print-electronic]. 2017 Apr 4/21/2017; 292(16): 6728-43. PMID: 28258218, PMCID: PMC5399120, PII: M117.778308, DOI: 10.1074/jbc.M117.778308, ISSN: 1083-351X.

Marsch GA, Carlson BT, Guengerich FP. 7,8-benzoflavone binding to human cytochrome P450 3A4 reveals complex fluorescence quenching, suggesting binding at multiple protein sites. J. Biomol. Struct. Dyn [print-electronic]. 2017 Mar 3/20/2017; 1-20. PMID: 28278026, DOI: 10.1080/07391102.2017.1301270, ISSN: 1538-0254.

Guengerich FP. Intersection of the Roles of Cytochrome P450 Enzymes with Xenobiotic and Endogenous Substrates: Relevance to Toxicity and Drug Interactions. Chem. Res. Toxicol [print-electronic]. 2017 Jan 1/17/2017; 30(1): 2-12. PMID: 27472660, PMCID: PMC5293730, DOI: 10.1021/acs.chemrestox.6b00226, ISSN: 1520-5010.

Cohen SM, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IM, Smith RL, Bastaki M, Harman CL, McGowen MM, Valerio LG, Taylor SV. Safety evaluation of substituted thiophenes used as flavoring ingredients. Food Chem. Toxicol [print-electronic]. 2017 Jan; 99: 40-59. PMID: 27836750, PII: S0278-6915(16)30392-1, DOI: 10.1016/j.fct.2016.10.023, ISSN: 1873-6351.

Murayama N, Kazuki Y, Satoh D, Arata K, Harada T, Shibata N, Guengerich FP, Yamazaki H. Induction of human cytochrome P450 3A enzymes in cultured placental cells by thalidomide and relevance to bioactivation and toxicity. J Toxicol Sci. 2017; 42(3): 343-8. PMID: 28496040, DOI: 10.2131/jts.42.343, ISSN: 1880-3989.

Guengerich FP. Kinetic Deuterium Isotope Effects in Cytochrome P450 Reactions. Meth. Enzymol [print-electronic]. 2017; 596: 217-38. PMID: 28911772, PMCID: PMC5776663, PII: S0076-6879(17)30219-7, DOI: 10.1016/bs.mie.2017.06.036, ISSN: 1557-7988.

Yamazaki H, Suemizu H, Mitsui M, Shimizu M, Guengerich FP. Combining Chimeric Mice with Humanized Liver, Mass Spectrometry, and Physiologically-Based Pharmacokinetic Modeling in Toxicology. Chem. Res. Toxicol [print-electronic]. 2016 Dec 12/19/2016; 29(12): 1903-11. PMID: 27337115, PMCID: PMC5292139, DOI: 10.1021/acs.chemrestox.6b00136, ISSN: 1520-5010.

Shimada T, Kakimoto K, Takenaka S, Koga N, Uehara S, Murayama N, Yamazaki H, Kim D, Guengerich FP, Komori M. Roles of Human CYP2A6 and Monkey CYP2A24 and 2A26 Cytochrome P450 Enzymes in the Oxidation of 2,5,2',5'-Tetrachlorobiphenyl. Drug Metab. Dispos [print-electronic]. 2016 Dec; 44(12): 1899-909. PMID: 27625140, PII: dmd.116.072991, DOI: 10.1124/dmd.116.072991, ISSN: 1521-009X.

Cohen SM, Fukushima S, Gooderham NJ, Guengerich FP, Hecht SS, Rietjens IM, Smith RL, Bastaki M, Harman CL, McGowen MM, Taylor SV. FEMA expert panel review of p-mentha-1,8-dien-7-al genotoxicity testing results. Food Chem. Toxicol [print-electronic]. 2016 Dec; 98(Pt B): 201-9. PMID: 27773699, PII: S0278-6915(16)30379-9, DOI: 10.1016/j.fct.2016.10.020, ISSN: 1873-6351.

Patra A, Zhang Q, Guengerich FP, Egli M. Mechanisms of Insertion of dCTP and dTTP Opposite the DNA Lesion O6-Methyl-2'-deoxyguanosine by Human DNA Polymerase ¿. J. Biol. Chem [print-electronic]. 2016 Nov 11/11/2016; 291(46): 24304-13. PMID: 27694439, PMCID: PMC5104950, PII: M116.755462, DOI: 10.1074/jbc.M116.755462, ISSN: 1083-351X.

Wickramaratne S, Ji S, Mukherjee S, Su Y, Pence MG, Lior-Hoffmann L, Fu I, Broyde S, Guengerich FP, Distefano M, Schärer OD, Sham YY, Tretyakova N. Bypass of DNA-Protein Cross-links Conjugated to the 7-Deazaguanine Position of DNA by Translesion Synthesis Polymerases. J. Biol. Chem [print-electronic]. 2016 Nov 11/4/2016; 291(45): 23589-603. PMID: 27621316, PMCID: PMC5095413, PII: M116.745257, DOI: 10.1074/jbc.M116.745257, ISSN: 1083-351X.

Kim JK, Yeom M, Hong JK, Song I, Lee YS, Guengerich FP, Choi JY. Six Germline Genetic Variations Impair the Translesion Synthesis Activity of Human DNA Polymerase ¿. Chem. Res. Toxicol [print-electronic]. 2016 Oct 10/17/2016; 29(10): 1741-54. PMID: 27603496, PMCID: PMC5116392, DOI: 10.1021/acs.chemrestox.6b00244, ISSN: 1520-5010.

Federspiel JD, Codreanu SG, Goyal S, Albertolle ME, Lowe E, Teague J, Wong H, Guengerich FP, Liebler DC. Specificity of Protein Covalent Modification by the Electrophilic Proteasome Inhibitor Carfilzomib in Human Cells. Mol. Cell Proteomics [print-electronic]. 2016 Oct; 15(10): 3233-42. PMID: 27503896, PMCID: PMC5054346, PII: M116.059709, DOI: 10.1074/mcp.M116.059709, ISSN: 1535-9484.

Choi JY, Patra A, Yeom M, Lee YS, Zhang Q, Egli M, Guengerich FP. Kinetic and Structural Impact of Metal Ions and Genetic Variations on Human DNA Polymerase ¿. J. Biol. Chem [print-electronic]. 2016 Sep 9/30/2016; 291(40): 21063-73. PMID: 27555320, PMCID: PMC5076516, PII: M116.748285, DOI: 10.1074/jbc.M116.748285, ISSN: 1083-351X.

Guengerich FP. Metals in Biology 2016: Molecular Basis of Selection of Metals by Enzymes. J. Biol. Chem [print-electronic]. 2016 Sep 9/30/2016; 291(40): 20838-9. PMID: 27462079, PMCID: PMC5076496, PII: R116.749259, DOI: 10.1074/jbc.R116.749259, ISSN: 1083-351X.

Yoshimoto FK, Jung IJ, Goyal S, Gonzalez E, Guengerich FP. Isotope-Labeling Studies Support the Electrophilic Compound I Iron Active Species, FeO(3+), for the Carbon-Carbon Bond Cleavage Reaction of the Cholesterol Side-Chain Cleavage Enzyme, Cytochrome P450 11A1. J. Am. Chem. Soc [print-electronic]. 2016 Sep 9/21/2016; 138(37): 12124-41. PMID: 27571509, PMCID: PMC5289895, DOI: 10.1021/jacs.6b04437, ISSN: 1520-5126.

Yamazaki H, Suemizu H, Kazuki Y, Oofusa K, Kuribayashi S, Shimizu M, Ninomiya S, Horie T, Shibata N, Guengerich FP. Assessment of Protein Binding of 5-Hydroxythalidomide Bioactivated in Humanized Mice with Human P450 3A-Chromosome or Hepatocytes by Two-Dimensional Electrophoresis/Accelerator Mass Spectrometry. Chem. Res. Toxicol [print-electronic]. 2016 Aug 8/15/2016; 29(8): 1279-81. PMID: 27464947, PMCID: PMC5282975, DOI: 10.1021/acs.chemrestox.6b00210, ISSN: 1520-5010.

Yoshimoto FK, Gonzalez E, Auchus RJ, Guengerich FP. Mechanism of 17a,20-Lyase and New Hydroxylation Reactions of Human Cytochrome P450 17A1: 18O LABELING AND OXYGEN SURROGATE EVIDENCE FOR A ROLE OF A PERFERRYL OXYGEN. J. Biol. Chem [print-electronic]. 2016 Aug 8/12/2016; 291(33): 17143-64. PMID: 27339894, PMCID: PMC5016118, PII: M116.732966, DOI: 10.1074/jbc.M116.732966, ISSN: 1083-351X.

Savas Ü, Wei S, Hsu MH, Falck JR, Guengerich FP, Capdevila JH, Johnson EF. 20-Hydroxyeicosatetraenoic Acid (HETE)-dependent Hypertension in Human Cytochrome P450 (CYP) 4A11 Transgenic Mice: NORMALIZATION OF BLOOD PRESSURE BY SODIUM RESTRICTION, HYDROCHLOROTHIAZIDE, OR BLOCKADE OF THE TYPE 1 ANGIOTENSIN II RECEPTOR. J. Biol. Chem [print-electronic]. 2016 Aug 8/5/2016; 291(32): 16904-19. PMID: 27298316, PMCID: PMC4974402, PII: M116.732297, DOI: 10.1074/jbc.M116.732297, ISSN: 1083-351X.

O'Flaherty DK, Patra A, Su Y, Guengerich FP, Egli M, Wilds CJ. Lesion Orientation of O(4)-Alkylthymidine Influences Replication by Human DNA Polymerase ¿. Chem Sci [print-electronic]. 2016 Aug 8/1/2016; 7(8): 4896-904. PMID: 27574558, PMCID: PMC5001687, DOI: 10.1039/C6SC00666C, ISSN: 2041-6520.

Hargrove TY, Friggeri L, Wawrzak Z, Sivakumaran S, Yazlovitskaya EM, Hiebert SW, Guengerich FP, Waterman MR, Lepesheva GI. Human sterol 14a-demethylase as a target for anticancer chemotherapy: towards structure-aided drug design. J. Lipid Res [print-electronic]. 2016 Aug; 57(8): 1552-63. PMID: 27313059, PMCID: PMC4959870, PII: jlr.M069229, DOI: 10.1194/jlr.M069229, ISSN: 1539-7262.

Guengerich FP, Waterman MR, Egli M. Recent Structural Insights into Cytochrome P450 Function. Trends Pharmacol. Sci [print-electronic]. 2016 Aug; 37(8): 625-40. PMID: 27267697, PMCID: PMC4961565, PII: S0165-6147(16)30040-2, DOI: 10.1016/j.tips.2016.05.006, ISSN: 1873-3735.

Patra A, Su Y, Zhang Q, Johnson KM, Guengerich FP, Egli M. Structural and Kinetic Analysis of Miscoding Opposite the DNA Adduct 1,N6-Ethenodeoxyadenosine by Human Translesion DNA Polymerase ¿. J. Biol. Chem [print-electronic]. 2016 Jul 7/1/2016; 291(27): 14134-45. PMID: 27226627, PMCID: PMC4933172, PII: M116.732487, DOI: 10.1074/jbc.M116.732487, ISSN: 1083-351X.

Acimovic J, Goyal S, Košir R, Golicnik M, Perše M, Belic A, Urlep Ž, Guengerich FP, Rozman D. Cytochrome P450 metabolism of the post-lanosterol intermediates explains enigmas of cholesterol synthesis. Sci Rep. 2016 Jun 6/23/2016; 6: 28462. PMID: 27334049, PMCID: PMC4917857, PII: srep28462, DOI: 10.1038/srep28462, ISSN: 2045-2322.

Shimada T, Takenaka S, Kakimoto K, Murayama N, Lim YR, Kim D, Foroozesh MK, Yamazaki H, Guengerich FP, Komori M. Structure-Function Studies of Naphthalene, Phenanthrene, Biphenyl, and Their Derivatives in Interaction with and Oxidation by Cytochromes P450 2A13 and 2A6. Chem. Res. Toxicol [print-electronic]. 2016 Jun 6/20/2016; 29(6): 1029-40. PMID: 27137136, PMCID: PMC5293596, DOI: 10.1021/acs.chemrestox.6b00083, ISSN: 1520-5010.

Kramlinger VM, Nagy LD, Fujiwara R, Johnson KM, Phan TT, Xiao Y, Enright JM, Toomey MB, Corbo JC, Guengerich FP. Human cytochrome P450 27C1 catalyzes 3,4-desaturation of retinoids [letter]. FEBS Lett [print-electronic]. 2016 May; 590(9): 1304-12. PMID: 27059013, PMCID: PMC4864060, DOI: 10.1002/1873-3468.12167, ISSN: 1873-3468.

Humphreys WG, Will Y, Guengerich FP. Toxicology Strategies for Drug Discovery - Present and Future: Introduction [editorial]. Chem. Res. Toxicol. 2016 Apr 4/18/2016; 29(4): 437. PMID: 27087588, DOI: 10.1021/acs.chemrestox.6b00049, ISSN: 1520-5010.

Liu B, Xue Q, Tang Y, Cao J, Guengerich FP, Zhang H. Mechanisms of mutagenesis: DNA replication in the presence of DNA damage. Mutat Res Rev Mutat Res [print-electronic]. 2016 Apr; 768: 53-67. PMID: 27234563, PMCID: PMC5237373, PII: S1383-5742(16)30020-5, DOI: 10.1016/j.mrrev.2016.03.006, ISSN: 1388-2139.

Yeom M, Kim IH, Kim JK, Kang K, Eoff RL, Guengerich FP, Choi JY. Effects of Twelve Germline Missense Variations on DNA Lesion and G-Quadruplex Bypass Activities of Human DNA Polymerase REV1. Chem. Res. Toxicol [print-electronic]. 2016 Mar 3/21/2016; 29(3): 367-79. PMID: 26914252, PMCID: PMC5116390, DOI: 10.1021/acs.chemrestox.5b00513, ISSN: 1520-5010.

Shimada T, Takenaka S, Murayama N, Kramlinger VM, Kim JH, Kim D, Liu J, Foroozesh MK, Yamazaki H, Guengerich FP, Komori M. Oxidation of pyrene, 1-hydroxypyrene, 1-nitropyrene and 1-acetylpyrene by human cytochrome P450 2A13. Xenobiotica [print-electronic]. 2016 Mar; 46(3): 211-24. PMID: 26247835, PII: 10.3109/00498254.2015.1069419, DOI: 10.3109/00498254.2015.1069419, ISSN: 1366-5928.

Su Y, Egli M, Guengerich FP. Mechanism of Ribonucleotide Incorporation by Human DNA Polymerase ¿. J. Biol. Chem [print-electronic]. 2016 Jan 1/6/2016; PMID: 26740629, PII: M115.706226, DOI: 10.1074/jbc.M115.706226, ISSN: 1083-351X.

O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ. Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase ¿ opposite Intrastrand Cross-Linked DNA. Biochemistry [print-electronic]. 2015 Dec 12/29/2015; 54(51): 7449-56. PMID: 26624500, DOI: 10.1021/acs.biochem.5b01078, ISSN: 1520-4995.

Xue Q, Zhong M, Liu B, Tang Y, Wei Z, Guengerich FP, Zhang H. Kinetic analysis of bypass of 7,8-dihydro-8-oxo-2'-deoxyguanosine by the catalytic core of yeast DNA polymerase ¿. Biochimie [print-electronic]. 2015 Dec 12/15/2015; PMID: 26700143, PII: S0300-9084(15)00422-8, DOI: 10.1016/j.biochi.2015.12.009, ISSN: 1638-6183.

Enright JM, Toomey MB, Sato SY, Temple SE, Allen JR, Fujiwara R, Kramlinger VM, Nagy LD, Johnson KM, Xiao Y, How MJ, Johnson SL, Roberts NW, Kefalov VJ, Guengerich FP, Corbo JC. Cyp27c1 Red-Shifts the Spectral Sensitivity of Photoreceptors by Converting Vitamin A1 into A2. Curr. Biol [print-electronic]. 2015 Dec 12/7/2015; 25(23): 3048-57. PMID: 26549260, PII: S0960-9822(15)01246-4, DOI: 10.1016/j.cub.2015.10.018, ISSN: 1879-0445.

Nishiyama S, Suemizu H, Shibata N, Guengerich FP, Yamazaki H. Simulation of Human Plasma Concentrations of Thalidomide and Primary 5-Hydroxylated Metabolites Explored with Pharmacokinetic Data in Humanized TK-NOG Mice. Chem. Res. Toxicol [print-electronic]. 2015 Nov 11/16/2015; 28(11): 2088-90. PMID: 26492539, DOI: 10.1021/acs.chemrestox.5b00381, ISSN: 1520-5010.

Appella E, Jenkins LM, Guengerich FP. Introduction to Thematic Series: Protein Interactions, Structures, and Networks. J. Biol. Chem [print-electronic]. 2015 Oct 10/30/2015; 290(44): 26393-4. PMID: 26354433, PMCID: PMC4646296, PII: R115.690370, DOI: 10.1074/jbc.R115.690370, ISSN: 1083-351X.

Hargrove TY, Wawrzak Z, Lamb DC, Guengerich FP, Lepesheva GI. Structure-Functional Characterization of Cytochrome P450 Sterol 14a-Demethylase (CYP51B) from Aspergillus fumigatus and Molecular Basis for the Development of Antifungal Drugs. J. Biol. Chem [print-electronic]. 2015 Sep 9/25/2015; 290(39): 23916-34. PMID: 26269599, PMCID: PMC4583043, PII: M115.677310, DOI: 10.1074/jbc.M115.677310, ISSN: 1083-351X.

Pallan PS, Lei L, Wang C, Waterman MR, Guengerich FP, Egli M. Research Resource: Correlating Human Cytochrome P450 21A2 Crystal Structure and Phenotypes of Mutations in Congenital Adrenal Hyperplasia. Mol. Endocrinol [print-electronic]. 2015 Sep; 29(9): 1375-84. PMID: 26172259, PMCID: PMC4552440, DOI: 10.1210/ME.2015-1127, ISSN: 1944-9917.

Guengerich FP. Introduction: Metals in Biology: a-Ketoglutarate/Iron-Dependent Dioxygenases. J. Biol. Chem [print-electronic]. 2015 Aug 8/21/2015; 290(34): 20700-1. PMID: 26152720, PMCID: PMC4543631, PII: R115.675652, DOI: 10.1074/jbc.R115.675652, ISSN: 1083-351X.

Kramlinger VM, Alvarado Rojas M, Kanamori T, Guengerich FP. Cytochrome P450 3A Enzymes Catalyze the O6-Demethylation of Thebaine, a Key Step in Endogenous Mammalian Morphine Biosynthesis. J. Biol. Chem [print-electronic]. 2015 Aug 8/14/2015; 290(33): 20200-10. PMID: 26157146, PMCID: PMC4536429, PII: M115.665331, DOI: 10.1074/jbc.M115.665331, ISSN: 1083-351X.

Guengerich FP. Introduction: Metals in Biology: METALS AT THE HOST-PATHOGEN INTERFACE. J. Biol. Chem [print-electronic]. 2015 Jul 7/31/2015; 290(31): 18943-4. PMID: 26055725, PMCID: PMC4521014, PII: R115.670265, DOI: 10.1074/jbc.R115.670265, ISSN: 1083-351X.

Kotapati S, Wickramaratne S, Esades A, Boldry EJ, Quirk Dorr D, Pence MG, Guengerich FP, Tretyakova NY. Polymerase Bypass of N(6)-Deoxyadenosine Adducts Derived from Epoxide Metabolites of 1,3-Butadiene. Chem. Res. Toxicol [print-electronic]. 2015 Jul 7/20/2015; 28(7): 1496-507. PMID: 26098310, PMCID: PMC4704788, DOI: 10.1021/acs.chemrestox.5b00166, ISSN: 1520-5010.

Su Y, Patra A, Harp JM, Egli M, Guengerich FP. Roles of Residues Arg-61 and Gln-38 of Human DNA Polymerase ¿ in Bypass of Deoxyguanosine and 7,8-Dihydro-8-oxo-2'-deoxyguanosine. J. Biol. Chem [print-electronic]. 2015 Jun 6/26/2015; 290(26): 15921-33. PMID: 25947374, PMCID: PMC4481197, PII: M115.653691, DOI: 10.1074/jbc.M115.653691, ISSN: 1083-351X.

Chang SC, Fedeles BI, Wu J, Delaney JC, Li D, Zhao L, Christov PP, Yau E, Singh V, Jost M, Drennan CL, Marnett LJ, Rizzo CJ, Levine SS, Guengerich FP, Essigmann JM. Next-generation sequencing reveals the biological significance of the N(2),3-ethenoguanine lesion in vivo. Nucleic Acids Res [print-electronic]. 2015 Jun 6/23/2015; 43(11): 5489-500. PMID: 25837992, PMCID: PMC4477646, PII: gkv243, DOI: 10.1093/nar/gkv243, ISSN: 1362-4962.

Bojic M, Sedgeman CA, Nagy LD, Guengerich FP. Aromatic hydroxylation of salicylic acid and aspirin by human cytochromes P450. Eur J Pharm Sci [print-electronic]. 2015 Jun 6/20/2015; 73: 49-56. PMID: 25840124, PMCID: PMC4414920, PII: S0928-0987(15)00115-3, DOI: 10.1016/j.ejps.2015.03.015, ISSN: 1879-0720.

Zdzalik D, Domanska A, Prorok P, Kosicki K, van den Born E, Falnes PØ, Rizzo CJ, Guengerich FP, Tudek B. Differential repair of etheno-DNA adducts by bacterial and human AlkB proteins. DNA Repair (Amst.) [print-electronic]. 2015 Jun; 30: 1-10. PMID: 25797601, PMCID: PMC4451939, PII: S1568-7864(15)00057-9, DOI: 10.1016/j.dnarep.2015.02.021, ISSN: 1568-7856.

Pallan PS, Wang C, Lei L, Yoshimoto FK, Auchus RJ, Waterman MR, Guengerich FP, Egli M. Human Cytochrome P450 21A2, the Major Steroid 21-Hydroxylase: STRUCTURE OF THE ENZYME·PROGESTERONE SUBSTRATE COMPLEX AND RATE-LIMITING C-H BOND CLEAVAGE. J. Biol. Chem [print-electronic]. 2015 May 5/22/2015; 290(21): 13128-43. PMID: 25855791, PMCID: PMC4505568, PII: M115.646307, DOI: 10.1074/jbc.M115.646307, ISSN: 1083-351X.

Lajovic A, Nagy LD, Guengerich FP, Bren U. Carcinogenesis of urethane: simulation versus experiment. Chem. Res. Toxicol [print-electronic]. 2015 Apr 4/20/2015; 28(4): 691-701. PMID: 25642734, DOI: 10.1021/tx500459t, ISSN: 1520-5010.

Patra A, Zhang Q, Lei L, Su Y, Egli M, Guengerich FP. Structural and kinetic analysis of nucleoside triphosphate incorporation opposite an abasic site by human translesion DNA polymerase ¿. J. Biol. Chem [print-electronic]. 2015 Mar 3/27/2015; 290(13): 8028-38. PMID: 25666608, PMCID: PMC4375460, PII: M115.637561, DOI: 10.1074/jbc.M115.637561, ISSN: 1083-351X.

Chowdhury G, Guengerich FP. Characterization of thioether-linked protein adducts of DNA using a Raney-Ni-mediated desulfurization method and liquid chromatography-electrospray-tandem mass spectrometry. Curr Protoc Nucleic Acid Chem. 2015 Mar 3/9/2015; 60: 10.15.1-10.15.14. PMID: 25754888, PMCID: PMC4374425, DOI: 10.1002/0471142700.nc1015s60, ISSN: 1934-9289.

Shimada T, Takenaka S, Murayama N, Yamazaki H, Kim JH, Kim D, Yoshimoto FK, Guengerich FP, Komori M. Oxidation of Acenaphthene and Acenaphthylene by Human Cytochrome P450 Enzymes. Chem. Res. Toxicol. 2015 Feb 2/16/2015; 28(2): 268-78. PMID: 25642975, DOI: 10.1021/tx500505y, ISSN: 1520-5010.

Pallan PS, Nagy LD, Lei L, Gonzalez E, Kramlinger VM, Azumaya CM, Wawrzak Z, Waterman MR, Guengerich FP, Egli M. Structural and kinetic basis of steroid 17a,20-lyase activity in teleost fish cytochrome P450 17A1 and its absence in cytochrome P450 17A2. J. Biol. Chem [print-electronic]. 2015 Feb 2/6/2015; 290(6): 3248-68. PMID: 25533464, PMCID: PMC4318999, PII: M114.627265, DOI: 10.1074/jbc.M114.627265, ISSN: 1083-351X.

Rendic S, Guengerich FP. Survey of Human Oxidoreductases and Cytochrome P450 Enzymes Involved in the Metabolism of Xenobiotic and Natural Chemicals. Chem. Res. Toxicol [print-electronic]. 2015 Jan 1/20/2015; 28(1): 38-42. PMID: 25485457, PMCID: PMC4303333, DOI: 10.1021/tx500444e, ISSN: 1520-5010.

Bajpai P, Srinivasan S, Ghosh J, Nagy LD, Wei S, Guengerich FP, Avadhani NG. Targeting of splice variants of human cytochrome P450 2C8 (CYP2C8) to mitochondria and their role in arachidonic acid metabolism and respiratory dysfunction. J. Biol. Chem [print-electronic]. 2014 Oct 10/24/2014; 289(43): 29614-30. PMID: 25160618, PMCID: PMC4207977, PII: M114.583062, DOI: 10.1074/jbc.M114.583062, ISSN: 1083-351X.

Yoshimoto FK, Guengerich FP. Mechanism of the third oxidative step in the conversion of androgens to estrogens by cytochrome P450 19A1 steroid aromatase. J. Am. Chem. Soc [print-electronic]. 2014 Oct 10/22/2014; 136(42): 15016-25. PMID: 25252141, PMCID: PMC4210144, DOI: 10.1021/ja508185d, ISSN: 1520-5126.

Guengerich FP. Thematic minireview series: metals in biology 2014. J. Biol. Chem [print-electronic]. 2014 Oct 10/10/2014; 289(41): 28094. PMID: 25160633, PMCID: PMC4192463, PII: R114.607093, DOI: 10.1074/jbc.R114.607093, ISSN: 1083-351X.

Kim D, Cha GS, Nagy LD, Yun CH, Guengerich FP. Kinetic analysis of lauric acid hydroxylation by human cytochrome P450 4A11. Biochemistry [print-electronic]. 2014 Oct 10/7/2014; 53(39): 6161-72. PMID: 25203493, PMCID: PMC4188250, DOI: 10.1021/bi500710e, ISSN: 1520-4995.

Zhao L, Pence MG, Eoff RL, Yuan S, Fercu CA, Guengerich FP. Elucidation of kinetic mechanisms of human translesion DNA polymerase ¿ using tryptophan mutants. FEBS J [print-electronic]. 2014 Oct; 281(19): 4394-410. PMID: 25065501, PMCID: PMC4182141, DOI: 10.1111/febs.12947, ISSN: 1742-4658.

Goyal S, Xiao Y, Porter NA, Xu L, Guengerich FP. Oxidation of 7-dehydrocholesterol and desmosterol by human cytochrome P450 46A1. J. Lipid Res [print-electronic]. 2014 Sep; 55(9): 1933-43. PMID: 25017465, PMCID: PMC4617353, PII: jlr.M051508, DOI: 10.1194/jlr.M051508, ISSN: 0022-2275.

Bojic M, Barbero L, Dolgos H, Freisleben A, Gallemann D, Riva S, Guengerich FP. Time- and NADPH-dependent inhibition of cytochrome P450 3A4 by the cyclopentapeptide cilengitide: significance of the guanidine group and accompanying spectral changes. Drug Metab. Dispos [print-electronic]. 2014 Sep; 42(9): 1438-46. PMID: 24985702, PII: dmd.114.059295, DOI: 10.1124/dmd.114.059295, ISSN: 1521-009X.

Patra A, Nagy LD, Zhang Q, Su Y, Müller L, Guengerich FP, Egli M. Kinetics, structure, and mechanism of 8-Oxo-7,8-dihydro-2'-deoxyguanosine bypass by human DNA polymerase ¿. J. Biol. Chem [print-electronic]. 2014 Jun 6/13/2014; 289(24): 16867-82. PMID: 24759104, PMCID: PMC4059130, PII: M114.551820, DOI: 10.1074/jbc.M114.551820, ISSN: 1083-351X.

Björkhem I, Diczfalusy U, Lövgren-Sandblom A, Starck L, Jonsson M, Tallman K, Schirmer H, Ousager LB, Crick PJ, Wang Y, Griffiths WJ, Guengerich FP. On the formation of 7-ketocholesterol from 7-dehydrocholesterol in patients with CTX and SLO. J. Lipid Res [print-electronic]. 2014 Apr 4/25/2014; 55(6): 1165-72. PMID: 24771866, PMCID: PMC4031947, PII: jlr.P048603, DOI: 10.1194/jlr.P048603, ISSN: 0022-2275.

Siller M, Goyal S, Yoshimoto FK, Xiao Y, Wei S, Guengerich FP. Oxidation of endogenous N-arachidonoylserotonin by human cytochrome P450 2U1. J. Biol. Chem [print-electronic]. 2014 Apr 4/11/2014; 289(15): 10476-87. PMID: 24563460, PMCID: PMC4036169, PII: M114.550004, DOI: 10.1074/jbc.M114.550004, ISSN: 1083-351X.

Murayama N, van Beuningen R, Suemizu H, Guillouzo CG, Shibata N, Yajima K, Utoh M, Shimizu M, Chesné C, Nakamura M, Guengerich FP, Houtman R, Yamazaki H. Thalidomide increases human hepatic cytochrome P450 3A enzymes by direct activation of the pregnane X receptor. Chem. Res. Toxicol [print-electronic]. 2014 Feb 2/17/2014; 27(2): 304-8. PMID: 24460184, PMCID: PMC4020009, DOI: 10.1021/tx4004374, ISSN: 1520-5010.

Chowdhury G, Shibata N, Yamazaki H, Guengerich FP. Human cytochrome P450 oxidation of 5-hydroxythalidomide and pomalidomide, an amino analogue of thalidomide. Chem. Res. Toxicol [print-electronic]. 2014 Jan 1/21/2014; 27(1): 147-56. PMID: 24350712, PMCID: PMC3929586, DOI: 10.1021/tx4004215, ISSN: 1520-5010.

Maddukuri L, Shuck SC, Eoff RL, Zhao L, Rizzo CJ, Guengerich FP, Marnett LJ. Replication, repair, and translesion polymerase bypass of N6-oxopropenyl-2'-deoxyadenosine. Biochemistry [print-electronic]. 2013 Dec 12/3/2013; 52(48): 8766-76. PMID: 24171480, PMCID: PMC3925671, DOI: 10.1021/bi401103k, ISSN: 1520-4995.

Chowdhury G, Cho SH, Pegg AE, Guengerich FP. Detection and characterization of 1,2-dibromoethane-derived DNA crosslinks formed with O(6) -alkylguanine-DNA alkyltransferase. Angew. Chem. Int. Ed. Engl [print-electronic]. 2013 Dec 12/2/2013; 52(49): 12879-82. PMID: 24130045, PMCID: PMC4048925, DOI: 10.1002/anie.201307580, ISSN: 1521-3773.

Cho SH, Guengerich FP. In vivo roles of conjugation with glutathione and O6-alkylguanine DNA-alkyltransferase in the mutagenicity of the bis-electrophiles 1,2-dibromoethane and 1,2,3,4-diepoxybutane in mice. Chem. Res. Toxicol [print-electronic]. 2013 Nov 11/18/2013; 26(11): 1765-74. PMID: 24191644, PMCID: PMC3889014, DOI: 10.1021/tx4003534, ISSN: 1520-5010.

Guengerich FP. Kinetic deuterium isotope effects in cytochrome P450 oxidation reactions. J Labelled Comp Radiopharm [print-electronic]. 2013 Jul; 56(9-10): 428-31. PMID: 24285515, DOI: 10.1002/jlcr.3031, ISSN: 1099-1344.

Cho SH, Guengerich FP. Replication past the butadiene diepoxide-derived DNA adduct S-[4-(N(6)-deoxyadenosinyl)-2,3-dihydroxybutyl]glutathione by DNA polymerases. Chem. Res. Toxicol [print-electronic]. 2013 Jun 6/17/2013; 26(6): 1005-13. PMID: 23701509, PMCID: PMC4049171, DOI: 10.1021/tx400145e, ISSN: 1520-5010.

Wang K, Guengerich FP. Reduction of aromatic and heterocyclic aromatic N-hydroxylamines by human cytochrome P450 2S1. Chem. Res. Toxicol [print-electronic]. 2013 Jun 6/17/2013; 26(6): 993-1004. PMID: 23682735, PMCID: PMC3707514, DOI: 10.1021/tx400139p, ISSN: 1520-5010.

Guengerich FP, Munro AW. Unusual cytochrome p450 enzymes and reactions. J. Biol. Chem [print-electronic]. 2013 Jun 6/14/2013; 288(24): 17065-73. PMID: 23632016, PMCID: PMC3682512, PII: R113.462275, DOI: 10.1074/jbc.R113.462275, ISSN: 1083-351X.

Guengerich FP. New trends in cytochrome p450 research at the half-century mark. J. Biol. Chem [print-electronic]. 2013 Jun 6/14/2013; 288(24): 17063-4. PMID: 23632015, PMCID: PMC3682511, PII: R113.466821, DOI: 10.1074/jbc.R113.466821, ISSN: 1083-351X.

Guengerich FP. Thematic minireview series: metals in biology 2013. J. Biol. Chem [print-electronic]. 2013 May 5/10/2013; 288(19): 13164. PMID: 23539620, PMCID: PMC3650354, PII: R113.467712, DOI: 10.1074/jbc.R113.467712, ISSN: 1083-351X.

Shimada T, Murayama N, Yamazaki H, Tanaka K, Takenaka S, Komori M, Kim D, Guengerich FP. Metabolic activation of polycyclic aromatic hydrocarbons and aryl and heterocyclic amines by human cytochromes P450 2A13 and 2A6. Chem. Res. Toxicol [print-electronic]. 2013 Apr 4/15/2013; 26(4): 529-37. PMID: 23432465, PMCID: PMC3713097, DOI: 10.1021/tx3004906, ISSN: 1520-5010.

Shimada T, Kim D, Murayama N, Tanaka K, Takenaka S, Nagy LD, Folkman LM, Foroozesh MK, Komori M, Yamazaki H, Guengerich FP. Binding of diverse environmental chemicals with human cytochromes P450 2A13, 2A6, and 1B1 and enzyme inhibition. Chem. Res. Toxicol [print-electronic]. 2013 Apr 4/15/2013; 26(4): 517-28. PMID: 23432429, PMCID: PMC4026275, DOI: 10.1021/tx300492j, ISSN: 1520-5010.

Tian Z, Cheng Q, Yoshimoto FK, Lei L, Lamb DC, Guengerich FP. Cytochrome P450 107U1 is required for sporulation and antibiotic production in Streptomyces coelicolor. Arch. Biochem. Biophys [print-electronic]. 2013 Feb 2/15/2013; 530(2): 101-7. PMID: 23357279, PMCID: PMC3600146, PII: S0003-9861(13)00006-4, DOI: 10.1016/j.abb.2013.01.001, ISSN: 1096-0384.

Cheng Q, Guengerich FP. High-throughput fluorescence assay of cytochrome P450 3A4. Methods Mol. Biol. 2013; 987: 157-62. PMID: 23475676, PMCID: PMC4024206, DOI: 10.1007/978-1-62703-321-3_14, ISSN: 1940-6029.

Cheng Q, Guengerich FP. Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches. Methods Mol. Biol. 2013; 987: 71-7. PMID: 23475668, PMCID: PMC3846702, DOI: 10.1007/978-1-62703-321-3_6, ISSN: 1940-6029.

Chowdhury G, Calcutt MW, Nagy LD, Guengerich FP. Oxidation of methyl and ethyl nitrosamines by cytochrome P450 2E1 and 2B1. Biochemistry [print-electronic]. 2012 Dec 12/18/2012; 51(50): 9995-10007. PMID: 23186213, PMCID: PMC3525961, DOI: 10.1021/bi301092c, ISSN: 1520-4995.

Zhao L, Pence MG, Christov PP, Wawrzak Z, Choi JY, Rizzo CJ, Egli M, Guengerich FP. Basis of miscoding of the DNA adduct N2,3-ethenoguanine by human Y-family DNA polymerases. J. Biol. Chem [print-electronic]. 2012 Oct 10/12/2012; 287(42): 35516-26. PMID: 22910910, PMCID: PMC3471744, PII: M112.403253, DOI: 10.1074/jbc.M112.403253, ISSN: 1083-351X.

Cheng Q, Sohl CD, Yoshimoto FK, Guengerich FP. Oxidation of dihydrotestosterone by human cytochromes P450 19A1 and 3A4. J. Biol. Chem [print-electronic]. 2012 Aug 8/24/2012; 287(35): 29554-67. PMID: 22773874, PMCID: PMC3436178, PII: M112.390047, DOI: 10.1074/jbc.M112.390047, ISSN: 1083-351X.

Wang K, Guengerich FP. Bioactivation of fluorinated 2-aryl-benzothiazole antitumor molecules by human cytochrome P450s 1A1 and 2W1 and deactivation by cytochrome P450 2S1. Chem. Res. Toxicol [print-electronic]. 2012 Aug 8/20/2012; 25(8): 1740-51. PMID: 22734839, PMCID: PMC3850770, DOI: 10.1021/tx3001994, ISSN: 1520-5010.

Christov PP, Yamanaka K, Choi JY, Takata K, Wood RD, Guengerich FP, Lloyd RS, Rizzo CJ. Replication of the 2,6-diamino-4-hydroxy-N(5)-(methyl)-formamidopyrimidine (MeFapy-dGuo) adduct by eukaryotic DNA polymerases. Chem. Res. Toxicol [print-electronic]. 2012 Aug 8/20/2012; 25(8): 1652-61. PMID: 22721435, PMCID: PMC3682502, DOI: 10.1021/tx300113e, ISSN: 1520-5010.

Xiao Y, Guengerich FP. Metabolomic analysis and identification of a role for the orphan human cytochrome P450 2W1 in selective oxidation of lysophospholipids. J. Lipid Res [print-electronic]. 2012 Aug; 53(8): 1610-7. PMID: 22591743, PMCID: PMC3540858, PII: jlr.M027185, DOI: 10.1194/jlr.M027185, ISSN: 0022-2275.

Cho SH, Guengerich FP. Mutation spectra of S-(2-hydroxy-3,4-epoxybutyl)glutathione: comparison with 1,3-butadiene and its metabolites in the Escherichia coli rpoB gene. Chem. Res. Toxicol [print-electronic]. 2012 Jul 7/16/2012; 25(7): 1522-30. PMID: 22670845, PMCID: PMC3848527, DOI: 10.1021/tx3002109, ISSN: 1520-5010.

Zhao L, Christov PP, Kozekov ID, Pence MG, Pallan PS, Rizzo CJ, Egli M, Guengerich FP. Replication of N2,3-ethenoguanine by DNA polymerases. Angew. Chem. Int. Ed. Engl [print-electronic]. 2012 May 5/29/2012; 51(22): 5466-9. PMID: 22488769, PMCID: PMC3671877, DOI: 10.1002/anie.201109004, ISSN: 1521-3773.

Guengerich FP. Thematic minireview series: Metals in Biology 2012. J. Biol. Chem [print-electronic]. 2012 Apr 4/20/2012; 287(17): 13508-9. PMID: 22389507, PMCID: PMC3340140, PII: R112.355933, DOI: 10.1074/jbc.R112.355933, ISSN: 1083-351X.

Zhao B, Lei L, Kagawa N, Sundaramoorthy M, Banerjee S, Nagy LD, Guengerich FP, Waterman MR. Three-dimensional structure of steroid 21-hydroxylase (cytochrome P450 21A2) with two substrates reveals locations of disease-associated variants. J. Biol. Chem [print-electronic]. 2012 Mar 3/23/2012; 287(13): 10613-22. PMID: 22262854, PMCID: PMC3323056, PII: M111.323501, DOI: 10.1074/jbc.M111.323501, ISSN: 1083-351X.

Cho SH, Guengerich FP. Conjugation of butadiene diepoxide with glutathione yields DNA adducts in vitro and in vivo. Chem. Res. Toxicol [print-electronic]. 2012 Mar 3/19/2012; 25(3): 706-12. PMID: 22181695, PMCID: PMC3850785, DOI: 10.1021/tx200471x, ISSN: 1520-5010.

Chowdhury G, Guengerich FP. Liquid chromatography-mass spectrometry analysis of DNA polymerase reaction products. Curr Protoc Nucleic Acid Chem. 2011 Dec; Chapter 7: Unit 7.16.1-11. PMID: 22147421, PMCID: PMC3678528, DOI: 10.1002/0471142700.nc0716s47, ISSN: 1934-9289.

Salamanca-Pinzón SG, Guengerich FP. A tricistronic human adrenodoxin reductase-adrenodoxin-cytochrome P450 27A1 vector system for substrate hydroxylation in Escherichia coli. Protein Expr. Purif [print-electronic]. 2011 Oct; 79(2): 231-6. PMID: 21621619, PMCID: PMC3155662, PII: S1046-5928(11)00122-7, DOI: 10.1016/j.pep.2011.05.008, ISSN: 1096-0279.

Shinkyo R, Xu L, Tallman KA, Cheng Q, Porter NA, Guengerich FP. Conversion of 7-dehydrocholesterol to 7-ketocholesterol is catalyzed by human cytochrome P450 7A1 and occurs by direct oxidation without an epoxide intermediate. J. Biol. Chem [print-electronic]. 2011 Sep 9/23/2011; 286(38): 33021-8. PMID: 21813643, PMCID: PMC3190903, PII: M111.282434, DOI: 10.1074/jbc.M111.282434, ISSN: 1083-351X.

Choi JY, Eoff RL, Pence MG, Wang J, Martin MV, Kim EJ, Folkmann LM, Guengerich FP. Roles of the four DNA polymerases of the crenarchaeon Sulfolobus solfataricus and accessory proteins in DNA replication. J. Biol. Chem [print-electronic]. 2011 Sep 9/9/2011; 286(36): 31180-93. PMID: 21784862, PMCID: PMC3173079, PII: M111.258038, DOI: 10.1074/jbc.M111.258038, ISSN: 1083-351X.

Guengerich FP, Cheng Q. Orphans in the human cytochrome P450 superfamily: approaches to discovering functions and relevance in pharmacology. Pharmacol. Rev [print-electronic]. 2011 Sep; 63(3): 684-99. PMID: 21737533, PMCID: PMC3141877, PII: pr.110.003525, DOI: 10.1124/pr.110.003525, ISSN: 1521-0081.

Guengerich FP. Thematic minireview series on biological applications of mass spectrometry. J. Biol. Chem [print-electronic]. 2011 Jul 7/22/2011; 286(29): 25417. PMID: 21632545, PMCID: PMC3138286, PII: R111.266700, DOI: 10.1074/jbc.R111.266700, ISSN: 1083-351X.

Shanmugam G, Kozekov ID, Guengerich FP, Rizzo CJ, Stone MP. 1,N2-Etheno-2'-deoxyguanosine adopts the syn conformation about the glycosyl bond when mismatched with deoxyadenosine. Chem. Res. Toxicol [print-electronic]. 2011 Jul 7/18/2011; 24(7): 1071-9. PMID: 21675798, PMCID: PMC3138413, DOI: 10.1021/tx200089v, ISSN: 1520-5010.

Xiao Y, Shinkyo R, Guengerich FP. Cytochrome P450 2S1 is reduced by NADPH-cytochrome P450 reductase. Drug Metab. Dispos [print-electronic]. 2011 Jun; 39(6): 944-6. PMID: 21430234, PMCID: PMC3100906, PII: dmd.111.039321, DOI: 10.1124/dmd.111.039321, ISSN: 1521-009X.

Shinkyo R, Guengerich FP. Inhibition of human cytochrome P450 3A4 by cholesterol. J. Biol. Chem [print-electronic]. 2011 May 5/27/2011; 286(21): 18426-33. PMID: 21471209, PMCID: PMC3099659, PII: M111.240457, DOI: 10.1074/jbc.M111.240457, ISSN: 1083-351X.

Guengerich FP, Sohl CD, Chowdhury G. Multi-step oxidations catalyzed by cytochrome P450 enzymes: Processive vs. distributive kinetics and the issue of carbonyl oxidation in chemical mechanisms. Arch. Biochem. Biophys [print-electronic]. 2011 Mar 3/1/2011; 507(1): 126-34. PMID: 20804723, PMCID: PMC3010332, PII: S0003-9861(10)00352-8, DOI: 10.1016/j.abb.2010.08.017, ISSN: 1096-0384.

Shinkyo R, Guengerich FP. Cytochrome P450 7A1 cholesterol 7alpha-hydroxylation: individual reaction steps in the catalytic cycle and rate-limiting ferric iron reduction. J. Biol. Chem [print-electronic]. 2011 Feb 2/11/2011; 286(6): 4632-43. PMID: 21147774, PMCID: PMC3039343, PII: M110.193409, DOI: 10.1074/jbc.M110.193409, ISSN: 1083-351X.

Guengerich FP. Mechanisms of drug toxicity and relevance to pharmaceutical development. Drug Metab. Pharmacokinet [print-electronic]. 2011; 26(1): 3-14. PMID: 20978361, PMCID: PMC4707670, PII: JST.JSTAGE/dmpk/DMPK-10-RV-062, ISSN: 1880-0920.

Guengerich FP, Tang Z, Cheng Q, Salamanca-Pinzón SG. Approaches to deorphanization of human and microbial cytochrome P450 enzymes. Biochim. Biophys. Acta [print-electronic]. 2011 Jan; 1814(1): 139-45. PMID: 20493973, PMCID: PMC2939962, PII: S1570-9639(10)00130-5, DOI: 10.1016/j.bbapap.2010.05.005, ISSN: 0006-3002.

Pence MG, Choi JY, Egli M, Guengerich FP. Structural basis for proficient incorporation of dTTP opposite O6-methylguanine by human DNA polymerase iota. J. Biol. Chem [print-electronic]. 2010 Dec 12/24/2010; 285(52): 40666-72. PMID: 20961860, PMCID: PMC3003365, PII: M110.183665, DOI: 10.1074/jbc.M110.183665, ISSN: 1083-351X.

Cheng Q, Lamb DC, Kelly SL, Lei L, Guengerich FP. Cyclization of a cellular dipentaenone by Streptomyces coelicolor cytochrome P450 154A1 without oxidation/reduction. J. Am. Chem. Soc. 2010 Nov 11/3/2010; 132(43): 15173-5. PMID: 20979426, PMCID: PMC3118511, DOI: 10.1021/ja107801v, ISSN: 1520-5126.

Cho SH, Loecken EM, Guengerich FP. Mutagenicity of a glutathione conjugate of butadiene diepoxide. Chem. Res. Toxicol [print-electronic]. 2010 Oct 10/18/2010; 23(10): 1544-6. PMID: 20879737, PMCID: PMC3848503, DOI: 10.1021/tx100304f, ISSN: 1520-5010.

Eoff RL, McGrath CE, Maddukuri L, Salamanca-Pinzón SG, Marquez VE, Marnett LJ, Guengerich FP, Egli M. Selective modulation of DNA polymerase activity by fixed-conformation nucleoside analogues. Angew. Chem. Int. Ed. Engl. 2010 Oct 10/4/2010; 49(41): 7481-5. PMID: 20814997, PMCID: PMC3011974, DOI: 10.1002/anie.201003168, ISSN: 1521-3773.

Maddukuri L, Eoff RL, Choi JY, Rizzo CJ, Guengerich FP, Marnett LJ. In vitro bypass of the major malondialdehyde- and base propenal-derived DNA adduct by human Y-family DNA polymerases ¿, ¿, and Rev1. Biochemistry. 2010 Sep 9/28/2010; 49(38): 8415-24. PMID: 20726503, PMCID: PMC2943251, DOI: 10.1021/bi1009024, ISSN: 1520-4995.

Tang Z, Guengerich FP. Dansylation of unactivated alcohols for improved mass spectral sensitivity and application to analysis of cytochrome P450 oxidation products in tissue extracts. Anal. Chem. 2010 Sep 9/15/2010; 82(18): 7706-12. PMID: 20795636, PMCID: PMC2941762, DOI: 10.1021/ac1015497, ISSN: 1520-6882.

Irimia A, Loukachevitch LV, Eoff RL, Guengerich FP, Egli M. Metal-ion dependence of the active-site conformation of the translesion DNA polymerase Dpo4 from Sulfolobus solfataricus. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun [print-electronic]. 2010 Sep 9/1/2010; 66(Pt 9): 1013-8. PMID: 20823515, PMCID: PMC2935216, PII: S1744309110029374, DOI: 10.1107/S1744309110029374, ISSN: 1744-3091.

Guengerich FP. Thematic minireview series: metals in biology 2010. J. Biol. Chem [print-electronic]. 2010 Aug 8/27/2010; 285(35): 26727. PMID: 20522544, PMCID: PMC2930669, PII: R110.150870, DOI: 10.1074/jbc.R110.150870, ISSN: 1083-351X.

Christov PP, Petrova KV, Shanmugam G, Kozekov ID, Kozekova A, Guengerich FP, Stone MP, Rizzo CJ. Comparison of the in vitro replication of the 7-(2-oxoheptyl)-1,N2-etheno-2'-deoxyguanosine and 1,N2-etheno-2'-deoxyguanosine lesions by Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4). Chem. Res. Toxicol. 2010 Aug 8/16/2010; 23(8): 1330-41. PMID: 20578729, PMCID: PMC2922457, DOI: 10.1021/tx100082e, ISSN: 1520-5010.

Christov PP, Chowdhury G, Garmendia CA, Wang F, Stover JS, Elmquist CE, Kozekova A, Angel KC, Turesky RJ, Stone MP, Guengerich FP, Rizzo CJ. The C8-2'-deoxyguanosine adduct of 2-amino-3-methylimidazo[1,2-d]naphthalene, a carbocyclic analogue of the potent mutagen 2-amino-3-methylimidazo[4,5-f]quinoline, is a block to replication in vitro. Chem. Res. Toxicol. 2010 Jun 6/21/2010; 23(6): 1076-88. PMID: 20377178, PMCID: PMC2891123, DOI: 10.1021/tx100053n, ISSN: 1520-5010.

Sohl CD, Guengerich FP. Kinetic analysis of the three-step steroid aromatase reaction of human cytochrome P450 19A1. J. Biol. Chem [print-electronic]. 2010 Jun 6/4/2010; 285(23): 17734-43. PMID: 20385561, PMCID: PMC2878537, PII: M110.123711, DOI: 10.1074/jbc.M110.123711, ISSN: 1083-351X.

Guengerich FP, Tang Z, Salamanca-Pinzón SG, Cheng Q. Characterizing proteins of unknown function: orphan cytochrome p450 enzymes as a paradigm. Mol. Interv. 2010 Jun; 10(3): 153-63. PMID: 20539034, PMCID: PMC2895278, PII: 10/3/153, DOI: 10.1124/mi.10.3.6, ISSN: 1543-2548.

Shanmugam G, Kozekov ID, Guengerich FP, Rizzo CJ, Stone MP. Structure of the 1,N(2)-etheno-2'-deoxyguanosine lesion in the 3'-G(epsilon dG)T-5' sequence opposite a one-base deletion. Biochemistry. 2010 Mar 3/30/2010; 49(12): 2615-26. PMID: 20201499, PMCID: PMC2844103, DOI: 10.1021/bi901516d, ISSN: 1520-4995.

Guengerich FP, Rendic S. Update Information on Drug Metabolism Systems-2009, Part I. Curr. Drug Metab [print-electronic]. 2010 Mar 3/18/2010; PMID: 20236049, PII: BSP/CDM/E-Pub/00046, ISSN: 1875-5453.

Chowdhury G, Calcutt MW, Guengerich FP. Oxidation of N-Nitrosoalkylamines by human cytochrome P450 2A6: sequential oxidation to aldehydes and carboxylic acids and analysis of reaction steps. J. Biol. Chem [print-electronic]. 2010 Mar 3/12/2010; 285(11): 8031-44. PMID: 20061389, PMCID: PMC2832954, PII: M109.088039, DOI: 10.1074/jbc.M109.088039, ISSN: 1083-351X.

Zhang H, Guengerich FP. Effect of N2-guanyl modifications on early steps in catalysis of polymerization by Sulfolobus solfataricus P2 DNA polymerase Dpo4 T239W. J. Mol. Biol [print-electronic]. 2010 Feb 2/5/2010; 395(5): 1007-18. PMID: 19969000, PMCID: PMC2814591, PII: S0022-2836(09)01465-X, DOI: 10.1016/j.jmb.2009.11.071, ISSN: 1089-8638.

Tang Z, Salamanca-Pinzón SG, Wu ZL, Xiao Y, Guengerich FP. Human cytochrome P450 4F11: heterologous expression in bacteria, purification, and characterization of catalytic function. Arch. Biochem. Biophys [print-electronic]. 2010 Feb 2/1/2010; 494(1): 86-93. PMID: 19932081, PMCID: PMC2812615, PII: S0003-9861(09)00388-9, DOI: 10.1016/j.abb.2009.11.017, ISSN: 1096-0384.

Eoff RL, Choi JY, Guengerich FP. Mechanistic Studies with DNA Polymerases Reveal Complex Outcomes following Bypass of DNA Damage. J Nucleic Acids. 2010; 2010: PMID: 20936119, PMCID: PMC2948923, DOI: 10.4061/2010/830473, ISSN: 2090-021X.

Guengerich FP, Rendic S. Update information on drug metabolism systems--2009, part I. Curr. Drug Metab. 2010 Jan; 11(1): 1-3. PMID: 20307256, ISSN: 1875-5453.

Guengerich FP. Cataloging the Repertoire of Nature's Blowtorch, P450. Chem. Biol. 2009 Dec 12/24/2009; 16(12): 1215-6. PMID: 20064429, PII: S1074-5521(09)00410-4, DOI: 10.1016/j.chembiol.2009.12.002, ISSN: 1879-1301.

Zhang H, Beckman JW, Guengerich FP. Frameshift deletion by Sulfolobus solfataricus P2 DNA polymerase Dpo4 T239W is selective for purines and involves normal conformational change followed by slow phosphodiester bond formation. J. Biol. Chem [print-electronic]. 2009 Dec 12/11/2009; 284(50): 35144-53. PMID: 19837980, PMCID: PMC2787375, PII: M109.067397, DOI: 10.1074/jbc.M109.067397, ISSN: 1083-351X.

Chowdhury G, Dostalek M, Hsu EL, Nguyen LP, Stec DF, Bradfield CA, Guengerich FP. Structural identification of Diindole agonists of the aryl hydrocarbon receptor derived from degradation of indole-3-pyruvic acid. Chem. Res. Toxicol. 2009 Dec; 22(12): 1905-12. PMID: 19860413, PMCID: PMC2801742, DOI: 10.1021/tx9000418, ISSN: 1520-5010.

Deweese JE, Guengerich FP, Burgin AB, Osheroff N. Metal ion interactions in the DNA cleavage/ligation active site of human topoisomerase IIalpha. Biochemistry. 2009 Sep 9/29/2009; 48(38): 8940-7. PMID: 19697956, PMCID: PMC2782489, DOI: 10.1021/bi900875c, ISSN: 1520-4995.

Zhang H, Bren U, Kozekov ID, Rizzo CJ, Stec DF, Guengerich FP. Steric and electrostatic effects at the C2 atom substituent influence replication and miscoding of the DNA deamination product deoxyxanthosine and analogs by DNA polymerases. J. Mol. Biol [print-electronic]. 2009 Sep 9/18/2009; 392(2): 251-69. PMID: 19607842, PMCID: PMC2755575, PII: S0022-2836(09)00841-9, DOI: 10.1016/j.jmb.2009.07.019, ISSN: 1089-8638.

Irimia A, Eoff RL, Guengerich FP, Egli M. Structural and functional elucidation of the mechanism promoting error-prone synthesis by human DNA polymerase kappa opposite the 7,8-dihydro-8-oxo-2'-deoxyguanosine adduct. J. Biol. Chem [print-electronic]. 2009 Aug 8/14/2009; 284(33): 22467-80. PMID: 19542228, PMCID: PMC2755968, PII: M109.003905, DOI: 10.1074/jbc.M109.003905, ISSN: 0021-9258.

Eoff RL, Stafford JB, Szekely J, Rizzo CJ, Egli M, Guengerich FP, Marnett LJ. Structural and functional analysis of Sulfolobus solfataricus Y-family DNA polymerase Dpo4-catalyzed bypass of the malondialdehyde-deoxyguanosine adduct. Biochemistry. 2009 Aug 8/4/2009; 48(30): 7079-88. PMID: 19492857, PMCID: PMC2717710, DOI: 10.1021/bi9003588, ISSN: 1520-4995.

Eoff RL, Sanchez-Ponce R, Guengerich FP. Conformational changes during nucleotide selection by Sulfolobus solfataricus DNA polymerase Dpo4. J. Biol. Chem [print-electronic]. 2009 Jul 7/31/2009; 284(31): 21090-9. PMID: 19515847, PMCID: PMC2742873, PII: M109.009506, DOI: 10.1074/jbc.M109.009506, ISSN: 1083-351X.

Guengerich FP. Thematic minireview series: metals in biology. J. Biol. Chem [print-electronic]. 2009 Jul 7/10/2009; 284(28): 18557. PMID: 19363036, PMCID: PMC2707207, PII: M109.003863, DOI: 10.1074/jbc.M109.003863, ISSN: 0021-9258.

Voehler MW, Eoff RL, McDonald WH, Guengerich FP, Stone MP. Modulation of the structure, catalytic activity, and fidelity of African swine fever virus DNA polymerase X by a reversible disulfide switch. J. Biol. Chem [print-electronic]. 2009 Jul 7/3/2009; 284(27): 18434-44. PMID: 19419958, PMCID: PMC2709365, PII: M109.012542, DOI: 10.1074/jbc.M109.012542, ISSN: 1083-351X.

Chowdhury G, Guengerich FP. Tandem mass spectrometry-based detection of c4'-oxidized abasic sites at specific positions in DNA fragments. Chem. Res. Toxicol. 2009 Jul; 22(7): 1310-9. PMID: 19496605, PMCID: PMC3838791, DOI: 10.1021/tx900115z, ISSN: 1520-5010.

Zhang H, Eoff RL, Kozekov ID, Rizzo CJ, Egli M, Guengerich FP. Structure-function relationships in miscoding by Sulfolobus solfataricus DNA polymerase Dpo4: guanine N2,N2-dimethyl substitution produces inactive and miscoding polymerase complexes. J. Biol. Chem. 2009 Jun 6/26/2009; 284(26): 17687-99. PMID: 19542237, PMCID: PMC2719408, PII: 284/26/17687, DOI: 10.1074/jbc.M109014274, ISSN: 0021-9258.

Christov PP, Angel KC, Guengerich FP, Rizzo CJ. Replication past the N5-methyl-formamidopyrimidine lesion of deoxyguanosine by DNA polymerases and an improved procedure for sequence analysis of in vitro bypass products by mass spectrometry. Chem. Res. Toxicol. 2009 Jun; 22(6): 1086-95. PMID: 19397282, PMCID: PMC2754150, DOI: 10.1021/tx900047c, ISSN: 1520-5010.

Loecken EM, Dasari S, Hill S, Tabb DL, Guengerich FP. The bis-electrophile diepoxybutane cross-links DNA to human histones but does not result in enhanced mutagenesis in recombinant systems. Chem. Res. Toxicol. 2009 Jun; 22(6): 1069-76. PMID: 19364102, PMCID: PMC2696559, DOI: 10.1021/tx900037u, ISSN: 1520-5010.

Brown KL, Bren U, Stone MP, Guengerich FP. Inherent stereospecificity in the reaction of aflatoxin B(1) 8,9-epoxide with deoxyguanosine and efficiency of DNA catalysis. Chem. Res. Toxicol. 2009 May; 22(5): 913-7. PMID: 19301826, PMCID: PMC3141577, DOI: 10.1021/tx900002g, ISSN: 1520-5010.

Tang Z, Martin MV, Guengerich FP. Elucidation of functions of human cytochrome P450 enzymes: identification of endogenous substrates in tissue extracts using metabolomic and isotopic labeling approaches. Anal. Chem. 2009 Apr 4/15/2009; 81(8): 3071-8. PMID: 19301915, PMCID: PMC3354960, DOI: 10.1021/ac900021a, ISSN: 1520-6882.

Zhang H, Eoff RL, Kozekov ID, Rizzo CJ, Egli M, Guengerich FP. Versatility of Y-family Sulfolobus solfataricus DNA polymerase Dpo4 in translesion synthesis past bulky N2-alkylguanine adducts. J. Biol. Chem [print-electronic]. 2009 Feb 2/6/2009; 284(6): 3563-76. PMID: 19059910, PMCID: PMC2636699, PII: M807778200, DOI: 10.1074/jbc.M807778200, ISSN: 0021-9258.

Knutson CG, Rubinson EH, Akingbade D, Anderson CS, Stec DF, Petrova KV, Kozekov ID, Guengerich FP, Rizzo CJ, Marnett LJ. Oxidation and glycolytic cleavage of etheno and propano DNA base adducts. Biochemistry. 2009 Feb 2/3/2009; 48(4): 800-9. PMID: 19132922, PMCID: PMC2975463, PII: 10.1021/bi801654j, DOI: 10.1021/bi801654j, ISSN: 1520-4995.

Stafford JB, Eoff RL, Kozekova A, Rizzo CJ, Guengerich FP, Marnett LJ. Translesion DNA synthesis by human DNA polymerase eta on templates containing a pyrimidopurinone deoxyguanosine adduct, 3-(2'-deoxy-beta-d-erythro-pentofuranosyl)pyrimido-[1,2-a]purin-10(3H)-one. Biochemistry. 2009 Jan 1/20/2009; 48(2): 471-80. PMID: 19108641, PMCID: PMC2651650, PII: 10.1021/bi801591a, DOI: 10.1021/bi801591a, ISSN: 1520-4995.

Guengerich FP. Thematic series: metals in biology. J. Biol. Chem [print-electronic]. 2009 Jan 1/9/2009; 284(2): 709. PMID: 18757360, PII: R800060200, DOI: 10.1074/jbc.R800060200, ISSN: 0021-9258.

Cheng Q, Sohl CD, Guengerich FP. High-throughput fluorescence assay of cytochrome P450 3A4. Nat Protoc [print-electronic]. 2009; 4(9): 1258-61. PMID: 19661996, PMCID: PMC3843962, PII: nprot.2009.123, DOI: 10.1038/nprot.2009.123, ISSN: 1750-2799.

Sohl CD, Cheng Q, Guengerich FP. Chromatographic assays of drug oxidation by human cytochrome P450 3A4. Nat Protoc [print-electronic]. 2009; 4(9): 1252-7. PMID: 19661995, PMCID: PMC3883453, PII: nprot.2009.122, DOI: 10.1038/nprot.2009.122, ISSN: 1750-2799.

Guengerich FP, Martin MV, Sohl CD, Cheng Q. Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase. Nat Protoc [print-electronic]. 2009; 4(9): 1245-51. PMID: 19661994, PMCID: PMC3843963, PII: nprot.2009.121, DOI: 10.1038/nprot.2009.121, ISSN: 1750-2799.

Beckman JW, Wang Q, Guengerich FP. Kinetic analysis of correct nucleotide insertion by a Y-family DNA polymerase reveals conformational changes both prior to and following phosphodiester bond formation as detected by tryptophan fluorescence. J. Biol. Chem [print-electronic]. 2008 Dec 12/26/2008; 283(52): 36711-23. PMID: 18984592, PMCID: PMC2605995, PII: M806785200, DOI: 10.1074/jbc.M806785200, ISSN: 0021-9258.

Stark K, Wu ZL, Bartleson CJ, Guengerich FP. MRNA distribution and heterologous expression of orphan cytochrome P450 20A1. Drug Metab. Dispos [print-electronic]. 2008 Sep; 36(9): 1930-7. PMID: 18541694, PII: dmd.108.022020, DOI: 10.1124/dmd.108.022020, ISSN: 1521-009X.

Shanmugam G, Kozekov ID, Guengerich FP, Rizzo CJ, Stone MP. Structure of the 1,N2-ethenodeoxyguanosine adduct opposite cytosine in duplex DNA: Hoogsteen base pairing at pH 5.2. Chem. Res. Toxicol [print-electronic]. 2008 Sep; 21(9): 1795-805. PMID: 18693701, PMCID: PMC2646752, DOI: 10.1021/tx8001466, ISSN: 1520-5010.

Stark K, Dostalek M, Guengerich FP. Expression and purification of orphan cytochrome P450 4X1 and oxidation of anandamide. FEBS J. 2008 Jul; 275(14): 3706-17. PMID: 18549450, PII: EJB6518, DOI: 10.1111/j.1742-4658.2008.06518.x, ISSN: 1742-464X.

Dostalek M, Hardy KD, Milne GL, Morrow JD, Chen C, Gonzalez FJ, Gu J, Ding X, Johnson DA, Johnson JA, Martin MV, Guengerich FP. Development of oxidative stress by cytochrome P450 induction in rodents is selective for barbiturates and related to loss of pyridine nucleotide-dependent protective systems. J. Biol. Chem [print-electronic]. 2008 Jun 6/20/2008; 283(25): 17147-57. PMID: 18442974, PMCID: PMC2427356, PII: M802447200, DOI: 10.1074/jbc.M802447200, ISSN: 0021-9258.

Isin EM, Sohl CD, Eoff RL, Guengerich FP. Cooperativity of cytochrome P450 1A2: interactions of 1,4-phenylene diisocyanide and 1-isopropoxy-4-nitrobenzene. Arch. Biochem. Biophys [print-electronic]. 2008 May 5/1/2008; 473(1): 69-75. PMID: 18328798, PMCID: PMC4662254, PII: S0003-9861(08)00106-9, DOI: 10.1016/j.abb.2008.02.033, ISSN: 1096-0384.

Bodreddigari S, Jones LK, Egner PA, Groopman JD, Sutter CH, Roebuck BD, Guengerich FP, Kensler TW, Sutter TR. Protection against aflatoxin B1-induced cytotoxicity by expression of the cloned aflatoxin B1-aldehyde reductases rat AKR7A1 and human AKR7A3. Chem. Res. Toxicol [print-electronic]. 2008 May; 21(5): 1134-42. PMID: 18416522, PMCID: PMC3838790, DOI: 10.1021/tx7004458, ISSN: 1520-5010.

Sohl CD, Isin EM, Eoff RL, Marsch GA, Stec DF, Guengerich FP. Cooperativity in oxidation reactions catalyzed by cytochrome P450 1A2: highly cooperative pyrene hydroxylation and multiphasic kinetics of ligand binding. J. Biol. Chem [print-electronic]. 2008 Mar 3/14/2008; 283(11): 7293-308. PMID: 18187423, PII: M709783200, DOI: 10.1074/jbc.M709783200, ISSN: 0021-9258.

Loecken EM, Guengerich FP. Reactions of glyceraldehyde 3-phosphate dehydrogenase sulfhydryl groups with bis-electrophiles produce DNA-protein cross-links but not mutations. Chem. Res. Toxicol [print-electronic]. 2008 Feb; 21(2): 453-8. PMID: 18163542, PMCID: PMC4049368, DOI: 10.1021/tx7003618, ISSN: 0893-228X.

Chowdhury G, Guengerich FP. Direct detection and mapping of sites of base modification in DNA fragments by tandem mass spectrometry. Angew. Chem. Int. Ed. Engl. 2008; 47(2): 381-4. PMID: 18022988, DOI: 10.1002/anie.200703942, ISSN: 1521-3773.

Guengerich FP. Cytochrome p450 and chemical toxicology. Chem. Res. Toxicol [print-electronic]. 2008 Jan; 21(1): 70-83. PMID: 18052394, DOI: 10.1021/tx700079z, ISSN: 0893-228X.

Irimia A, Eoff RL, Pallan PS, Guengerich FP, Egli M. Structure and activity of Y-class DNA polymerase DPO4 from Sulfolobus solfataricus with templates containing the hydrophobic thymine analog 2,4-difluorotoluene. J. Biol. Chem [print-electronic]. 2007 Dec 12/14/2007; 282(50): 36421-33. PMID: 17951245, PII: M707267200, DOI: 10.1074/jbc.M707267200, ISSN: 0021-9258.

Dostalek M, Brooks JD, Hardy KD, Milne GL, Moore MM, Sharma S, Morrow JD, Guengerich FP. In vivo oxidative damage in rats is associated with barbiturate response but not other cytochrome P450 inducers. Mol. Pharmacol [print-electronic]. 2007 Dec; 72(6): 1419-24. PMID: 17898314, PII: mol.107.040238, DOI: 10.1124/mol.107.040238, ISSN: 1521-0111.

Watanabe K, Liberman RG, Skipper PL, Tannenbaum SR, Guengerich FP. Analysis of DNA adducts formed in vivo in rats and mice from 1,2-dibromoethane, 1,2-dichloroethane, dibromomethane, and dichloromethane using HPLC/accelerator mass spectrometry and relevance to risk estimates. Chem. Res. Toxicol [print-electronic]. 2007 Nov; 20(11): 1594-600. PMID: 17907789, DOI: 10.1021/tx700125p, ISSN: 0893-228X.

Shanmugam G, Goodenough AK, Kozekov ID, Guengerich FP, Rizzo CJ, Stone MP. Structure of the 1,N2-etheno-2'-deoxyguanosine adduct in duplex DNA at pH 8.6. Chem. Res. Toxicol [print-electronic]. 2007 Nov; 20(11): 1601-11. PMID: 17941687, PMCID: PMC2546360, DOI: 10.1021/tx7001788, ISSN: 0893-228X.

Eoff RL, Irimia A, Angel KC, Egli M, Guengerich FP. Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4. J. Biol. Chem [print-electronic]. 2007 Jul 7/6/2007; 282(27): 19831-43. PMID: 17468100, PII: M702290200, DOI: 10.1074/jbc.M702290200, ISSN: 0021-9258.

Chun YJ, Shimada T, Sanchez-Ponce R, Martin MV, Lei L, Zhao B, Kelly SL, Waterman MR, Lamb DC, Guengerich FP. Electron transport pathway for a Streptomyces cytochrome P450: cytochrome P450 105D5-catalyzed fatty acid hydroxylation in Streptomyces coelicolor A3(2). J. Biol. Chem [print-electronic]. 2007 Jun 6/15/2007; 282(24): 17486-500. PMID: 17446171, PII: M700863200, DOI: 10.1074/jbc.M700863200, ISSN: 0021-9258.

Eoff RL, Angel KC, Egli M, Guengerich FP. Molecular basis of selectivity of nucleoside triphosphate incorporation opposite O6-benzylguanine by sulfolobus solfataricus DNA polymerase Dpo4: steady-state and pre-steady-state kinetics and x-ray crystallography of correct and incorrect pairing. J. Biol. Chem [print-electronic]. 2007 May 5/4/2007; 282(18): 13573-84. PMID: 17337730, PII: M700656200, DOI: 10.1074/jbc.M700656200, ISSN: 0021-9258.

Sanchez-Ponce R, Guengerich FP. Untargeted analysis of mass spectrometry data for elucidation of metabolites and function of enzymes. Anal. Chem [print-electronic]. 2007 May 5/1/2007; 79(9): 3355-62. PMID: 17408243, PMCID: PMC3357132, DOI: 10.1021/ac0622781, ISSN: 0003-2700.

Isin EM, Guengerich FP. Multiple sequential steps involved in the binding of inhibitors to cytochrome P450 3A4. J. Biol. Chem [print-electronic]. 2007 Mar 3/2/2007; 282(9): 6863-74. PMID: 17200113, PII: M610346200, DOI: 10.1074/jbc.M610346200, ISSN: 0021-9258.

Guengerich FP, MacDonald JS. Applying mechanisms of chemical toxicity to predict drug safety. Chem. Res. Toxicol [print-electronic]. 2007 Mar; 20(3): 344-69. PMID: 17302443, DOI: 10.1021/tx600260a, ISSN: 0893-228X.

Shimada T, Murayama N, Okada K, Funae Y, Yamazaki H, Guengerich FP. Different mechanisms for inhibition of human cytochromes P450 1A1, 1A2, and 1B1 by polycyclic aromatic inhibitors. Chem. Res. Toxicol [print-electronic]. 2007 Mar; 20(3): 489-96. PMID: 17291012, DOI: 10.1021/tx600299p, ISSN: 0893-228X.

Isin EM, Guengerich FP. Complex reactions catalyzed by cytochrome P450 enzymes. Biochim. Biophys. Acta [print-electronic]. 2007 Mar; 1770(3): 314-29. PMID: 17239540, PII: S0304-4165(06)00199-1, DOI: 10.1016/j.bbagen.2006.07.003, ISSN: 0006-3002.

Eoff RL, Irimia A, Egli M, Guengerich FP. Sulfolobus solfataricus DNA polymerase Dpo4 is partially inhibited by "wobble" pairing between O6-methylguanine and cytosine, but accurate bypass is preferred. J. Biol. Chem [print-electronic]. 2007 Jan 1/12/2007; 282(2): 1456-67. PMID: 17105728, PII: M609661200, DOI: 10.1074/jbc.M609661200, ISSN: 0021-9258.

Guengerich FP. Mechanisms of cytochrome P450 substrate oxidation: MiniReview. J. Biochem. Mol. Toxicol. 2007; 21(4): 163-8. PMID: 17936929, ISSN: 1095-6670.

Stark K, Guengerich FP. Characterization of orphan human cytochromes P450. Drug Metab. Rev. 2007; 39(2-3): 627-37. PMID: 17786643, PII: 781832530, DOI: 10.1080/03602530701467708, ISSN: 0360-2532.

Choi JY, Chowdhury G, Zang H, Angel KC, Vu CC, Peterson LA, Guengerich FP. Translesion synthesis across O6-alkylguanine DNA adducts by recombinant human DNA polymerases. J. Biol. Chem [print-electronic]. 2006 Dec 12/15/2006; 281(50): 38244-56. PMID: 17050527, PII: M608369200, DOI: 10.1074/jbc.M608369200, ISSN: 0021-9258.

Chun YJ, Shimada T, Waterman MR, Guengerich FP. Understanding electron transport systems of Streptomyces cytochrome P450. Biochem. Soc. Trans. 2006 Dec; 34(Pt 6): 1183-5. PMID: 17073781, PII: BST0341183, DOI: 10.1042/BST0341183, ISSN: 0300-5127.

Stover JS, Chowdhury G, Zang H, Guengerich FP, Rizzo CJ. Translesion synthesis past the C8- and N2-deoxyguanosine adducts of the dietary mutagen 2-Amino-3-methylimidazo[4,5-f]quinoline in the NarI recognition sequence by prokaryotic DNA polymerases. Chem. Res. Toxicol. 2006 Nov; 19(11): 1506-17. PMID: 17112239, PMCID: PMC3150502, DOI: 10.1021/tx0601455, ISSN: 0893-228X.

Guengerich FP. A malleable catalyst dominates the metabolism of drugs. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2006 Sep 9/12/2006; 103(37): 13565-6. PMID: 16954196, PMCID: PMC1564253, PII: 0606333103, DOI: 10.1073/pnas.0606333103, ISSN: 0027-8424.

Choi JY, Stover JS, Angel KC, Chowdhury G, Rizzo CJ, Guengerich FP. Biochemical basis of genotoxicity of heterocyclic arylamine food mutagens: Human DNA polymerase eta selectively produces a two-base deletion in copying the N2-guanyl adduct of 2-amino-3-methylimidazo[4,5-f]quinoline but not the C8 adduct at the NarI G3 site. J. Biol. Chem [print-electronic]. 2006 Sep 9/1/2006; 281(35): 25297-306. PMID: 16835218, PII: M605699200, DOI: 10.1074/jbc.M605699200, ISSN: 0021-9258.

Watanabe K, Guengerich FP. Limited reactivity of formyl chloride with glutathione and relevance to metabolism and toxicity of dichloromethane. Chem. Res. Toxicol. 2006 Aug; 19(8): 1091-6. PMID: 16918250, DOI: 10.1021/tx060087n, ISSN: 0893-228X.

Choi JY, Angel KC, Guengerich FP. Translesion synthesis across bulky N2-alkyl guanine DNA adducts by human DNA polymerase kappa. J. Biol. Chem [print-electronic]. 2006 Jul 7/28/2006; 281(30): 21062-72. PMID: 16751196, PII: M602246200, DOI: 10.1074/jbc.M602246200, ISSN: 0021-9258.

Choi JY, Zang H, Angel KC, Kozekov ID, Goodenough AK, Rizzo CJ, Guengerich FP. Translesion synthesis across 1,N2-ethenoguanine by human DNA polymerases. Chem. Res. Toxicol. 2006 Jun; 19(6): 879-86. PMID: 16780368, PMCID: PMC3130186, DOI: 10.1021/tx060051v, ISSN: 0893-228X.

Zang H, Chowdhury G, Angel KC, Harris TM, Guengerich FP. Translesion synthesis across polycyclic aromatic hydrocarbon diol epoxide adducts of deoxyadenosine by Sulfolobus solfataricus DNA polymerase Dpo4. Chem. Res. Toxicol. 2006 Jun; 19(6): 859-67. PMID: 16780366, DOI: 10.1021/tx060056s, ISSN: 0893-228X.

Wu ZL, Sohl CD, Shimada T, Guengerich FP. Recombinant enzymes overexpressed in bacteria show broad catalytic specificity of human cytochrome P450 2W1 and limited activity of human cytochrome P450 2S1. Mol. Pharmacol [print-electronic]. 2006 Jun; 69(6): 2007-14. PMID: 16551781, PII: mol.106.023648, DOI: 10.1124/mol.106.023648, ISSN: 0026-895X.

Irimia A, Zang H, Loukachevitch LV, Eoff RL, Guengerich FP, Egli M. Calcium is a cofactor of polymerization but inhibits pyrophosphorolysis by the Sulfolobus solfataricus DNA polymerase Dpo4. Biochemistry. 2006 May 5/16/2006; 45(19): 5949-56. PMID: 16681366, DOI: 10.1021/bi052511+, ISSN: 0006-2960.

Choi JY, Guengerich FP. Kinetic evidence for inefficient and error-prone bypass across bulky N2-guanine DNA adducts by human DNA polymerase iota. J. Biol. Chem [print-electronic]. 2006 May 5/5/2006; 281(18): 12315-24. PMID: 16527824, PII: M600112200, DOI: 10.1074/jbc.M600112200, ISSN: 0021-9258.

Isin EM, Guengerich FP. Kinetics and thermodynamics of ligand binding by cytochrome P450 3A4. J. Biol. Chem [print-electronic]. 2006 Apr 4/7/2006; 281(14): 9127-36. PMID: 16467307, PII: M511375200, DOI: 10.1074/jbc.M511375200, ISSN: 0021-9258.

Shimada T, Guengerich FP. Inhibition of human cytochrome P450 1A1-, 1A2-, and 1B1-mediated activation of procarcinogens to genotoxic metabolites by polycyclic aromatic hydrocarbons. Chem. Res. Toxicol. 2006 Feb; 19(2): 288-94. PMID: 16485905, DOI: 10.1021/tx050291v, ISSN: 0893-228X.

Guengerich FP. Interactions of carcinogen-bound DNA with individual DNA polymerases. Chem. Rev. 2006 Feb; 106(2): 420-52. PMID: 16464013, DOI: 10.1021/cr0404693, ISSN: 0009-2665.

Lamb DC, Guengerich FP, Kelly SL, Waterman MR. Exploiting Streptomyces coelicolor A3(2) P450s as a model for application in drug discovery. Expert Opin Drug Metab Toxicol. 2006 Feb; 2(1): 27-40. PMID: 16863466, DOI: 10.1517/17425255.2.1.27, ISSN: 1744-7607.

Zang H, Irimia A, Choi JY, Angel KC, Loukachevitch LV, Egli M, Guengerich FP. Efficient and high fidelity incorporation of dCTP opposite 7,8-dihydro-8-oxodeoxyguanosine by Sulfolobus solfataricus DNA polymerase Dpo4. J. Biol. Chem [print-electronic]. 2006 Jan 1/27/2006; 281(4): 2358-72. PMID: 16306039, PII: M510889200, DOI: 10.1074/jbc.M510889200, ISSN: 0021-9258.

Wu ZL, Bartleson CJ, Ham AJ, Guengerich FP. Heterologous expression, purification, and properties of human cytochrome P450 27C1. Arch. Biochem. Biophys [print-electronic]. 2006 Jan 1/1/2006; 445(1): 138-46. PMID: 16360114, PII: S0003-9861(05)00457-1, DOI: 10.1016/j.abb.2005.11.002, ISSN: 0003-9861.

Guengerich FP, Martin MV. Purification of cytochromes P450: products of bacterial recombinant expression systems. Methods Mol. Biol. 2006; 320: 31-7. PMID: 16719372, DOI: 10.1385/1-59259-998-2:31, ISSN: 1064-3745.

Guengerich FP. Cytochrome P450s and other enzymes in drug metabolism and toxicity. AAPS J. 2006; 8(1): E101-11. PMID: 16584116, PMCID: PMC2751428, DOI: 10.1208/aapsj080112, ISSN: 1550-7416.

Guengerich FP, Martin MV. Purification of Cytochromes P450 : Products of Bacterial Recombinant Expression Systems. Methods Mol. Biol. 2006; 320: 31-8. PMID: 27699677, DOI: 10.1385/1-59259-998-2:31, ISSN: 1940-6029.

Zhao B, Guengerich FP, Voehler M, Waterman MR. Role of active site water molecules and substrate hydroxyl groups in oxygen activation by cytochrome P450 158A2: a new mechanism of proton transfer. J. Biol. Chem [print-electronic]. 2005 Dec 12/23/2005; 280(51): 42188-97. PMID: 16239228, PII: M509220200, DOI: 10.1074/jbc.M509220200, ISSN: 0021-9258.

Wu ZL, Podust LM, Guengerich FP. Expansion of substrate specificity of cytochrome P450 2A6 by random and site-directed mutagenesis. J. Biol. Chem [print-electronic]. 2005 Dec 12/9/2005; 280(49): 41090-100. PMID: 16215230, PII: M508182200, DOI: 10.1074/jbc.M508182200, ISSN: 0021-9258.

Guengerich FP, Wu ZL, Bartleson CJ. Function of human cytochrome P450s: characterization of the orphans. Biochem. Biophys. Res. Commun [print-electronic]. 2005 Dec 12/9/2005; 338(1): 465-9. PMID: 16126164, PII: S0006-291X(05)01794-8, DOI: 10.1016/j.bbrc.2005.08.079, ISSN: 0006-291X.

Kim D, Wu ZL, Guengerich FP. Analysis of coumarin 7-hydroxylation activity of cytochrome P450 2A6 using random mutagenesis. J. Biol. Chem [print-electronic]. 2005 Dec 12/2/2005; 280(48): 40319-27. PMID: 16207711, PII: M508171200, DOI: 10.1074/jbc.M508171200, ISSN: 0021-9258.

Goodenough AK, Kozekov ID, Zang H, Choi JY, Guengerich FP, Harris TM, Rizzo CJ. Site specific synthesis and polymerase bypass of oligonucleotides containing a 6-hydroxy-3,5,6,7-tetrahydro-9H-imidazo[1,2-a]purin-9-one base, an intermediate in the formation of 1,N2-etheno-2'-deoxyguanosine. Chem. Res. Toxicol. 2005 Nov; 18(11): 1701-14. PMID: 16300379, PMCID: PMC3135970, DOI: 10.1021/tx050141k, ISSN: 0893-228X.

Guengerich FP. How I became a biochemist. IUBMB Life. 2005 Oct; 57(10): 705-7. PMID: 16223712, PII: MN8Q4746133212V1, DOI: 10.1080/15216540500264604, ISSN: 1521-6543.

Choi JY, Guengerich FP. Adduct size limits efficient and error-free bypass across bulky N2-guanine DNA lesions by human DNA polymerase eta. J. Mol. Biol. 2005 Sep 9/9/2005; 352(1): 72-90. PMID: 16061253, PII: S0022-2836(05)00764-3, DOI: 10.1016/j.jmb.2005.06.079, ISSN: 0022-2836.

Zang H, Fang Q, Pegg AE, Guengerich FP. Kinetic analysis of steps in the repair of damaged DNA by human O6-alkylguanine-DNA alkyltransferase. J. Biol. Chem [print-electronic]. 2005 Sep 9/2/2005; 280(35): 30873-81. PMID: 16000301, PII: M505283200, DOI: 10.1074/jbc.M505283200, ISSN: 0021-9258.

Zang H, Goodenough AK, Choi JY, Irimia A, Loukachevitch LV, Kozekov ID, Angel KC, Rizzo CJ, Egli M, Guengerich FP. DNA adduct bypass polymerization by Sulfolobus solfataricus DNA polymerase Dpo4: analysis and crystal structures of multiple base pair substitution and frameshift products with the adduct 1,N2-ethenoguanine. J. Biol. Chem [print-electronic]. 2005 Aug 8/19/2005; 280(33): 29750-64. PMID: 15965231, PII: M504756200, DOI: 10.1074/jbc.M504756200, ISSN: 0021-9258.

Guengerich FP. Reduction of cytochrome b5 by NADPH-cytochrome P450 reductase. Arch. Biochem. Biophys. 2005 Aug 8/15/2005; 440(2): 204-11. PMID: 16055078, PII: S0003-9861(05)00263-8, DOI: 10.1016/j.abb.2005.06.019, ISSN: 0003-9861.

Krauser JA, Guengerich FP. Cytochrome P450 3A4-catalyzed testosterone 6beta-hydroxylation stereochemistry, kinetic deuterium isotope effects, and rate-limiting steps. J. Biol. Chem [print-electronic]. 2005 May 5/20/2005; 280(20): 19496-506. PMID: 15772082, PII: M501854200, DOI: 10.1074/jbc.M501854200, ISSN: 0021-9258.

Liebler DC, Guengerich FP. Elucidating mechanisms of drug-induced toxicity. Nat Rev Drug Discov. 2005 May; 4(5): 410-20. PMID: 15864270, PII: nrd1720, DOI: 10.1038/nrd1720, ISSN: 1474-1776.

VanderVeen LA, Druckova A, Riggins JN, Sorrells JL, Guengerich FP, Marnett LJ. Differential DNA recognition and cleavage by EcoRI dependent on the dynamic equilibrium between the two forms of the malondialdehyde-deoxyguanosine adduct. Biochemistry. 2005 Apr 4/5/2005; 44(13): 5024-33. PMID: 15794640, DOI: 10.1021/bi0472898, ISSN: 0006-2960.

Yun CH, Kim KH, Calcutt MW, Guengerich FP. Kinetic analysis of oxidation of coumarins by human cytochrome P450 2A6. J. Biol. Chem [print-electronic]. 2005 Apr 4/1/2005; 280(13): 12279-91. PMID: 15665333, PII: M411019200, DOI: 10.1074/jbc.M411019200, ISSN: 0021-9258.

Zhao B, Guengerich FP, Bellamine A, Lamb DC, Izumikawa M, Lei L, Podust LM, Sundaramoorthy M, Kalaitzis JA, Reddy LM, Kelly SL, Moore BS, Stec D, Voehler M, Falck JR, Shimada T, Waterman MR. Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of Streptomyces coelicolor A3(2) cytochrome P450 158A2. J. Biol. Chem [print-electronic]. 2005 Mar 3/25/2005; 280(12): 11599-607. PMID: 15659395, PII: M410933200, DOI: 10.1074/jbc.M410933200, ISSN: 0021-9258.

Vélez-Cruz R, Riggins JN, Daniels JS, Cai H, Guengerich FP, Marnett LJ, Osheroff N. Exocyclic DNA lesions stimulate DNA cleavage mediated by human topoisomerase II alpha in vitro and in cultured cells. Biochemistry. 2005 Mar 3/15/2005; 44(10): 3972-81. PMID: 15751973, DOI: 10.1021/bi0478289, ISSN: 0006-2960.

Shimada T, Mernaugh RL, Guengerich FP. Interactions of mammalian cytochrome P450, NADPH-cytochrome P450 reductase, and cytochrome b(5) enzymes. Arch. Biochem. Biophys. 2005 Mar 3/1/2005; 435(1): 207-16. PMID: 15680923, PII: S0003-9861(04)00699-X, DOI: 10.1016/j.abb.2004.12.008, ISSN: 0003-9861.

Zang H, Harris TM, Guengerich FP. Kinetics of nucleotide incorporation opposite polycyclic aromatic hydrocarbon-DNA adducts by processive bacteriophage T7 DNA polymerase. Chem. Res. Toxicol. 2005 Feb; 18(2): 389-400. PMID: 15720147, DOI: 10.1021/tx049683c, ISSN: 0893-228X.

Guengerich FP. Principles of covalent binding of reactive metabolites and examples of activation of bis-electrophiles by conjugation. Arch. Biochem. Biophys. 2005 Jan 1/15/2005; 433(2): 369-78. PMID: 15581593, PII: S0003-9861(04)00436-9, DOI: 10.1016/j.abb.2004.07.035, ISSN: 0003-9861.

Zang H, Harris TM, Guengerich FP. Kinetics of nucleotide incorporation opposite DNA bulky guanine N2 adducts by processive bacteriophage T7 DNA polymerase (exonuclease-) and HIV-1 reverse transcriptase. J. Biol. Chem [print-electronic]. 2005 Jan 1/14/2005; 280(2): 1165-78. PMID: 15533946, PII: M405996200, DOI: 10.1074/jbc.M405996200, ISSN: 0021-9258.

Guengerich FP. Activation of alkyl halides by glutathione transferases. Meth. Enzymol. 2005; 401: 342-53. PMID: 16399396, PII: S0076-6879(05)01021-9, DOI: 10.1016/S0076-6879(05)01021-9, ISSN: 0076-6879.

Guengerich FP. Life and times in biochemical toxicology. Int. J. Toxicol. 2005 Jan; 24(1): 5-21. PMID: 15981736, ISSN: 1091-5818.

Guengerich FP. Generation of reactive intermediates. J. Biochem. Mol. Toxicol. 2005; 19(3): 173-4. PMID: 15977199, DOI: 10.1002/jbt.20072, ISSN: 1095-6670.

Kim D, Guengerich FP. Cytochrome P450 activation of arylamines and heterocyclic amines. Annu. Rev. Pharmacol. Toxicol. 2005; 45: 27-49. PMID: 15822170, DOI: 10.1146/annurev.pharmtox.45.120403.100010, ISSN: 0362-1642.

Wu ZL, Aryal P, Lozach O, Meijer L, Guengerich FP. Biosynthesis of new indigoid inhibitors of protein kinases using recombinant cytochrome P450 2A6. Chem. Biodivers. 2005 Jan; 2(1): 51-65. PMID: 17191919, DOI: 10.1002/cbdv.200490166, ISSN: 1612-1880.

Kim D, Guengerich FP. Enhancement of 7-methoxyresorufin O-demethylation activity of human cytochrome P450 1A2 by molecular breeding. Arch. Biochem. Biophys. 2004 Dec 12/1/2004; 432(1): 102-8. PMID: 15519301, PII: S0003-9861(04)00501-6, DOI: 10.1016/j.abb.2004.09.001, ISSN: 0003-9861.

Guengerich FP. Where is toxicology headed in the future?. Drug Metab. Rev. 2004 Oct; 36(3-4): 475-86. PMID: 15554230, DOI: 10.1081/DMR-200033409, ISSN: 0360-2532.

Krauser JA, Voehler M, Tseng LH, Schefer AB, Godejohann M, Guengerich FP. Testosterone 1 beta-hydroxylation by human cytochrome P450 3A4. Eur. J. Biochem. 2004 Oct; 271(19): 3962-9. PMID: 15373842, PII: EJB4339, DOI: 10.1111/j.1432-1033.2004.04339.x, ISSN: 0014-2956.

Guengerich FP, Krauser JA, Johnson WW. Rate-limiting steps in oxidations catalyzed by rabbit cytochrome P450 1A2. Biochemistry. 2004 Aug 8/24/2004; 43(33): 10775-88. PMID: 15311939, DOI: 10.1021/bi0491393, ISSN: 0006-2960.

Valadez JG, Liu L, Loktionova NA, Pegg AE, Guengerich FP. Activation of bis-electrophiles to mutagenic conjugates by human O6-alkylguanine-DNA alkyltransferase. Chem. Res. Toxicol. 2004 Jul; 17(7): 972-82. PMID: 15257623, DOI: 10.1021/tx049897u, ISSN: 0893-228X.

Guengerich FP, Sorrells JL, Schmitt S, Krauser JA, Aryal P, Meijer L. Generation of new protein kinase inhibitors utilizing cytochrome p450 mutant enzymes for indigoid synthesis. J. Med. Chem. 2004 Jun 6/3/2004; 47(12): 3236-41. PMID: 15163202, DOI: 10.1021/jm030561b, ISSN: 0022-2623.

Guengerich FP. Cytochrome P450: what have we learned and what are the future issues?. Drug Metab. Rev. 2004 May; 36(2): 159-97. PMID: 15237850, DOI: 10.1081/DMR-120033996, ISSN: 0360-2532.

Choi JY, Guengerich FP. Analysis of the effect of bulk at N2-alkylguanine DNA adducts on catalytic efficiency and fidelity of the processive DNA polymerases bacteriophage T7 exonuclease- and HIV-1 reverse transcriptase. J. Biol. Chem [print-electronic]. 2004 Apr 4/30/2004; 279(18): 19217-29. PMID: 14985330, PII: M313759200, DOI: 10.1074/jbc.M313759200, ISSN: 0021-9258.

Valadez JG, Guengerich FP. S-(2-chloroethyl)glutathione-generated p53 mutation spectra are influenced by differential repair rates more than sites of initial dna damage. J. Biol. Chem [print-electronic]. 2004 Apr 4/2/2004; 279(14): 13435-46. PMID: 14715658, PII: M312358200, DOI: 10.1074/jbc.M312358200, ISSN: 0021-9258.

Kim D, Kadlubar FF, Teitel CH, Guengerich FP. Formation and reduction of aryl and heterocyclic nitroso compounds and significance in the flux of hydroxylamines. Chem. Res. Toxicol. 2004 Apr; 17(4): 529-36. PMID: 15089095, DOI: 10.1021/tx034267y, ISSN: 0893-228X.

Kim D, Guengerich FP. Selection of human cytochrome P450 1A2 mutants with enhanced catalytic activity for heterocyclic amine N-hydroxylation. Biochemistry. 2004 Feb 2/3/2004; 43(4): 981-8. PMID: 14744142, DOI: 10.1021/bi035593f, ISSN: 0006-2960.

Marsch GA, Botta S, Martin MV, McCormick WA, Guengerich FP. Formation and mass spectrometric analysis of DNA and nucleoside adducts by S-(1-acetoxymethyl)glutathione and by glutathione S-transferase-mediated activation of dihalomethanes. Chem. Res. Toxicol. 2004 Jan; 17(1): 45-54. PMID: 14727918, DOI: 10.1021/tx034156z, ISSN: 0893-228X.

Guengerich FP, McCormick WA, Wheeler JB. Analysis of the kinetic mechanism of haloalkane conjugation by mammalian theta-class glutathione transferases. Chem. Res. Toxicol. 2003 Nov; 16(11): 1493-9. PMID: 14615977, DOI: 10.1021/tx034157r, ISSN: 0893-228X.

Guengerich FP, Fang Q, Liu L, Hachey DL, Pegg AE. O6-alkylguanine-DNA alkyltransferase: low pKa and high reactivity of cysteine 145. Biochemistry. 2003 Sep 9/23/2003; 42(37): 10965-70. PMID: 12974631, DOI: 10.1021/bi034937z, ISSN: 0006-2960.

Guengerich FP. Cytochromes P450, drugs, and diseases. Mol. Interv. 2003 Jun; 3(4): 194-204. PMID: 14993447, PII: 3/4/194, DOI: 10.1124/mi.3.4.194, ISSN: 1534-0384.

Guengerich FP, Hanna IH, Martin MV, Gillam EM. Role of glutamic acid 216 in cytochrome P450 2D6 substrate binding and catalysis. Biochemistry. 2003 Feb 2/11/2003; 42(5): 1245-53. PMID: 12564927, DOI: 10.1021/bi027085w, ISSN: 0006-2960.

Guengerich FP. Activation of dihaloalkanes by thiol-dependent mechanisms. J. Biochem. Mol. Biol. 2003 Jan 1/31/2003; 36(1): 20-7. PMID: 12542971, ISSN: 1225-8687.

Guengerich FP. Cytochrome P450 oxidations in the generation of reactive electrophiles: epoxidation and related reactions. Arch. Biochem. Biophys. 2003 Jan 1/1/2003; 409(1): 59-71. PMID: 12464245, PII: S0003986102004150, ISSN: 0003-9861.

Guengerich FP, Wheeler JB, Chun YJ, Kim D, Shimada T, Aryal P, Oda Y, Gillam EM. Use of heterologously-expressed cytochrome P450 and glutathione transferase enzymes in toxicity assays. Toxicology. 2002 Dec 12/27/2002; 181-182: 261-4. PMID: 12505322, PII: S0300483X02002937, ISSN: 0300-483X.

Yoshioka T, Krauser JA, Guengerich FP. Microsomal oxidation of tribromoethylene and reactions of tribromoethylene oxide. Chem. Res. Toxicol. 2002 Nov; 15(11): 1414-20. PMID: 12437332, PII: tx020047t, ISSN: 0893-228X.

Guengerich FP, Miller GP, Hanna IH, Sato H, Martin MV. Oxidation of methoxyphenethylamines by cytochrome P450 2D6. Analysis of rate-limiting steps. J. Biol. Chem [print-electronic]. 2002 Sep 9/13/2002; 277(37): 33711-9. PMID: 12093814, PII: M205146200, DOI: 10.1074/jbc.M205146200, ISSN: 0021-9258.

Guengerich FP, Miller GP, Hanna IH, Martin MV, Léger S, Black C, Chauret N, Silva JM, Trimble LA, Yergey JA, Nicoll-Griffith DA. Diversity in the oxidation of substrates by cytochrome P450 2D6: lack of an obligatory role of aspartate 301-substrate electrostatic bonding. Biochemistry. 2002 Sep 9/10/2002; 41(36): 11025-34. PMID: 12206675, PII: bi020341k, ISSN: 0006-2960.

Guengerich FP. N-hydroxyarylamines. Drug Metab. Rev. 2002 Aug; 34(3): 607-23. PMID: 12214670, DOI: 10.1081/DMR-120005663, ISSN: 0360-2532.

Yoshioka T, Krauser JA, Guengerich FP. Tetrachloroethylene oxide: hydrolytic products and reactions with phosphate and lysine. Chem. Res. Toxicol. 2002 Aug; 15(8): 1096-105. PMID: 12184794, PII: tx020028j, ISSN: 0893-228X.

Guengerich FP, Voehler M, Williams KM, Deng Z, Harris TM. Structure of the aflatoxin B(1) dialdehyde adduct formed from reaction with methylamine. Chem. Res. Toxicol. 2002 Jun; 15(6): 793-8. PMID: 12067246, PII: tx0200055, ISSN: 0893-228X.

Guengerich FP, Arneson KO, Williams KM, Deng Z, Harris TM. Reaction of aflatoxin B(1) oxidation products with lysine. Chem. Res. Toxicol. 2002 Jun; 15(6): 780-92. PMID: 12067245, PII: tx010156s, ISSN: 0893-228X.

Guengerich FP. Cytochrome P450 enzymes in the generation of commercial products. Nat Rev Drug Discov. 2002 May; 1(5): 359-66. PMID: 12120411, DOI: 10.1038/nrd792, ISSN: 1474-1776.

Guengerich FP. Update information on human P450s. Drug Metab. Rev. 2002 Feb; 34(1-2): 7-15. PMID: 11996014, DOI: 10.1081/DMR-120001386, ISSN: 0360-2532.

Woodside AM, Guengerich FP. Effect of the O6 substituent on misincorporation kinetics catalyzed by DNA polymerases at O(6)-methylguanine and O(6)-benzylguanine. Biochemistry. 2002 Jan 1/22/2002; 41(3): 1027-38. PMID: 11790127, PII: bi011495n, ISSN: 0006-2960.

Woodside AM, Guengerich FP. Misincorporation and stalling at O(6)-methylguanine and O(6)-benzylguanine: evidence for inactive polymerase complexes. Biochemistry. 2002 Jan 1/22/2002; 41(3): 1039-50. PMID: 11790128, PII: bi011496f, ISSN: 0006-2960.

Kim D, Guengerich FP. Random mutagenesis by whole-plasmid PCR amplification. Methods Mol. Biol. 2002; 192: 241-5. PMID: 12494656, PII: 1-59259-177-9-241, DOI: 10.1385/1-59259-177-9:241, ISSN: 1064-3745.

Bell-Parikh LC, Hosea NA, Martin MV, Guengerich FP. Purification of cytochrome P-450 enzymes. Curr Protoc Toxicol. 2002; Chapter 4: Unit4.2. PMID: 23045082, DOI: 10.1002/0471140856.tx0402s12, ISSN: 1934-9262.

Miller GP, Hanna IH, Nishimura Y, Guengerich FP. Oxidation of phenethylamine derivatives by cytochrome P450 2D6: the issue of substrate protonation in binding and catalysis. Biochemistry. 2001 Nov 11/27/2001; 40(47): 14215-23. PMID: 11714275, PII: bi0110037, ISSN: 0006-2960.

Nakamura K, Martin MV, Guengerich FP. Random mutagenesis of human cytochrome p450 2A6 and screening with indole oxidation products. Arch. Biochem. Biophys. 2001 Nov 11/1/2001; 395(1): 25-31. PMID: 11673862, PII: S000398610192569X, DOI: 10.1006/abbi.2001.2569, ISSN: 0003-9861.

Hanna IH, Krauser JA, Cai H, Kim MS, Guengerich FP. Diversity in mechanisms of substrate oxidation by cytochrome P450 2D6. Lack of an allosteric role of NADPH-cytochrome P450 reductase in catalytic regioselectivity. J. Biol. Chem [print-electronic]. 2001 Oct 10/26/2001; 276(43): 39553-61. PMID: 11509577, PII: M106841200, DOI: 10.1074/jbc.M106841200, ISSN: 0021-9258.

Hanna IH, Kim MS, Guengerich FP. Heterologous expression of cytochrome P450 2D6 mutants, electron transfer, and catalysis of bufuralol hydroxylation: the role of aspartate 301 in structural integrity. Arch. Biochem. Biophys. 2001 Sep 9/15/2001; 393(2): 255-61. PMID: 11556812, PII: S0003-9861(01)92510-X, DOI: 10.1006/abbi.2001.2510, ISSN: 0003-9861.

Guengerich FP. Functional genomics and proteomics applied to the study of nutritional metabolism. Nutr. Rev. 2001 Aug; 59(8 Pt 1): 259-63. PMID: 11518180, ISSN: 0029-6643.

Wheeler JB, Stourman NV, Thier R, Dommermuth A, Vuilleumier S, Rose JA, Armstrong RN, Guengerich FP. Conjugation of haloalkanes by bacterial and mammalian glutathione transferases: mono- and dihalomethanes. Chem. Res. Toxicol. 2001 Aug; 14(8): 1118-27. PMID: 11511186, PII: tx010019v, ISSN: 0893-228X.

Wheeler JB, Stourman NV, Armstrong RN, Guengerich FP. Conjugation of haloalkanes by bacterial and mammalian glutathione transferases: mono- and vicinal dihaloethanes. Chem. Res. Toxicol. 2001 Aug; 14(8): 1107-17. PMID: 11511185, PII: tx0100183, ISSN: 0893-228X.

Guengerich FP. Forging the links between metabolism and carcinogenesis. Mutat. Res. 2001 Jul; 488(3): 195-209. PMID: 11397649, PII: S138357420100059X, ISSN: 0027-5107.

Miller GP, Guengerich FP. Binding and oxidation of alkyl 4-nitrophenyl ethers by rabbit cytochrome P450 1A2: evidence for two binding sites. Biochemistry. 2001 Jun 6/19/2001; 40(24): 7262-72. PMID: 11401574, PII: bi010402z, ISSN: 0006-2960.

Guengerich FP. Uncommon P450-catalyzed reactions. Curr. Drug Metab. 2001 Jun; 2(2): 93-115. PMID: 11469727, ISSN: 1389-2002.

Guengerich FP, Cai H, McMahon M, Hayes JD, Sutter TR, Groopman JD, Deng Z, Harris TM. Reduction of aflatoxin B1 dialdehyde by rat and human aldo-keto reductases. Chem. Res. Toxicol. 2001 Jun; 14(6): 727-37. PMID: 11409944, PII: tx010005p, ISSN: 0893-228X.

Guengerich FP. Common and uncommon cytochrome P450 reactions related to metabolism and chemical toxicity. Chem. Res. Toxicol. 2001 Jun; 14(6): 611-50. PMID: 11409933, PII: tx0002583, ISSN: 0893-228X.

Nakamura K, Hanna IH, Cai H, Nishimura Y, Williams KM, Guengerich FP. Coumarin substrates for cytochrome P450 2D6 fluorescence assays. Anal. Biochem. 2001 May 5/15/2001; 292(2): 280-6. PMID: 11355862, PII: S0003-2697(01)95098-5, DOI: 10.1006/abio.2001.5098, ISSN: 0003-2697.

Marsch GA, Mundkowski RG, Morris BJ, Manier ML, Hartman MK, Guengerich FP. Characterization of nucleoside and DNA adducts formed by S-(1-acetoxymethyl)glutathione and implications for dihalomethane-glutathione conjugates. Chem. Res. Toxicol. 2001 May; 14(5): 600-8. PMID: 11368561, PII: tx010006h, ISSN: 0893-228X.

Yun CH, Miller GP, Guengerich FP. Oxidations of p-alkoxyacylanilides catalyzed by human cytochrome P450 1A2: structure-activity relationships and simulation of rate constants of individual steps in catalysis. Biochemistry. 2001 Apr 4/10/2001; 40(14): 4521-30. PMID: 11284709, PII: bi002906n, ISSN: 0006-2960.

Cai H, Guengerich FP. Reaction of trichloroethylene and trichloroethylene oxide with cytochrome P450 enzymes: inactivation and sites of modification. Chem. Res. Toxicol. 2001 Apr; 14(4): 451-8. PMID: 11304134, PII: tx0002280, ISSN: 0893-228X.

Einolf HJ, Guengerich FP. Fidelity of nucleotide insertion at 8-oxo-7,8-dihydroguanine by mammalian DNA polymerase delta. Steady-state and pre-steady-state kinetic analysis. J. Biol. Chem [print-electronic]. 2001 Feb 2/9/2001; 276(6): 3764-71. PMID: 11110788, PII: M006696200, DOI: 10.1074/jbc.M006696200, ISSN: 0021-9258.

Guengerich FP, Cai H, Johnson WW, Parikh A. Reactive intermediates in biological systems: what have we learned and where are we going?. Adv. Exp. Med. Biol. 2001; 500: 639-50. PMID: 11765010, ISSN: 0065-2598.

Cai H, Guengerich FP. Reaction of trichloroethylene oxide with proteins and dna: instability of adducts and modulation of functions. Chem. Res. Toxicol. 2001 Jan; 14(1): 54-61. PMID: 11170508, PII: tx000185n, ISSN: 0893-228X.

Yun CH, Miller GP, Guengerich FP. Rate-determining steps in phenacetin oxidations by human cytochrome P450 1A2 and selected mutants. Biochemistry. 2000 Sep 9/19/2000; 39(37): 11319-29. PMID: 10985777, PII: bi000869u, ISSN: 0006-2960.

Guengerich FP, Parikh A, Yun CH, Kim D, Nakamura K, Notley LM, Gillam EM. What makes P450s work? Searches for answers with known and new P450s. Drug Metab. Rev. 2000 Aug; 32(3-4): 267-81. PMID: 11139129, DOI: 10.1081/DMR-100102334, ISSN: 0360-2532.

Wandel C, Kim RB, Guengerich FP, Wood AJ. Mibefradil is a P-glycoprotein substrate and a potent inhibitor of both P-glycoprotein and CYP3A in vitro. Drug Metab. Dispos. 2000 Aug; 28(8): 895-8. PMID: 10901697, ISSN: 0090-9556.

Hanna IH, Dawling S, Roodi N, Guengerich FP, Parl FF. Cytochrome P450 1B1 (CYP1B1) pharmacogenetics: association of polymorphisms with functional differences in estrogen hydroxylation activity. Cancer Res. 2000 Jul 7/1/2000; 60(13): 3440-4. PMID: 10910054, ISSN: 0008-5472.

Einolf HJ, Guengerich FP. Kinetic analysis of nucleotide incorporation by mammalian DNA polymerase delta. J. Biol. Chem. 2000 May 5/26/2000; 275(21): 16316-22. PMID: 10748013, PII: M001291200, DOI: 10.1074/jbc.M001291200, ISSN: 0021-9258.

Hosea NA, Miller GP, Guengerich FP. Elucidation of distinct ligand binding sites for cytochrome P450 3A4. Biochemistry. 2000 May 5/23/2000; 39(20): 5929-39. PMID: 10821664, PII: bi992765t, ISSN: 0006-2960.

Cai H, Guengerich FP. Acylation of protein lysines by trichloroethylene oxide. Chem. Res. Toxicol. 2000 May; 13(5): 327-35. PMID: 10813648, PII: tx000003p, ISSN: 0893-228X.

Plastaras JP, Guengerich FP, Nebert DW, Marnett LJ. Xenobiotic-metabolizing cytochromes P450 convert prostaglandin endoperoxide to hydroxyheptadecatrienoic acid and the mutagen, malondialdehyde. J. Biol. Chem. 2000 Apr 4/21/2000; 275(16): 11784-90. PMID: 10766802, ISSN: 0021-9258.

Hanna IH, Reed JR, Guengerich FP, Hollenberg PF. Expression of human cytochrome P450 2B6 in Escherichia coli: characterization of catalytic activity and expression levels in human liver. Arch. Biochem. Biophys. 2000 Apr 4/1/2000; 376(1): 206-16. PMID: 10729207, PII: S0003-9861(00)91708-9, DOI: 10.1006/abbi.2000.1708, ISSN: 0003-9861.

Guengerich FP. Metabolism of chemical carcinogens. Carcinogenesis. 2000 Mar; 21(3): 345-51. PMID: 10688854, ISSN: 0143-3334.

Guengerich FP, Mundkowski RG, Voehler M, Kadlubar FF. Formation and reactions of N(7)-aminoguanosine and derivatives. Chem. Res. Toxicol. 1999 Oct; 12(10): 906-16. PMID: 10525265, PII: tx990094u, ISSN: 0893-228X.

Bell-Parikh LC, Guengerich FP. Kinetics of cytochrome P450 2E1-catalyzed oxidation of ethanol to acetic acid via acetaldehyde. J. Biol. Chem. 1999 Aug 8/20/1999; 274(34): 23833-40. PMID: 10446146, ISSN: 0021-9258.

Guengerich FP, Parikh A, Turesky RJ, Josephy PD. Inter-individual differences in the metabolism of environmental toxicants: cytochrome P450 1A2 as a prototype. Mutat. Res. 1999 Jul 7/16/1999; 428(1-2): 115-24. PMID: 10517985, PII: S1383-5742(99)00039-3, ISSN: 0027-5107.

Akasaka S, Guengerich FP. Mutagenicity of site-specifically located 1,N2-ethenoguanine in Chinese hamster ovary cell chromosomal DNA. Chem. Res. Toxicol. 1999 Jun; 12(6): 501-7. PMID: 10368312, PII: tx980259j, DOI: 10.1021/tx980259j, ISSN: 0893-228X.

Parikh A, Josephy PD, Guengerich FP. Selection and characterization of human cytochrome P450 1A2 mutants with altered catalytic properties. Biochemistry. 1999 Apr 4/27/1999; 38(17): 5283-9. PMID: 10220313, PII: bi990142+, DOI: 10.1021/bi990142+, ISSN: 0006-2960.

Furge LL, Guengerich FP. Explanation of pre-steady-state kinetics and decreased burst amplitude of HIV-1 reverse transcriptase at sites of modified DNA bases with an additional, nonproductive enzyme-DNA-nucleotide complex. Biochemistry. 1999 Apr 4/13/1999; 38(15): 4818-25. PMID: 10200170, PII: bi982163u, DOI: 10.1021/bi982163u, ISSN: 0006-2960.

Guengerich FP, Johnson WW. Kinetics of hydrolysis and reaction of aflatoxin B1 exo-8,9-epoxide and relevance to toxicity and detoxication. Drug Metab. Rev. 1999 Feb; 31(1): 141-58. PMID: 10065369, DOI: 10.1081/DMR-100101911, ISSN: 0360-2532.

Guengerich FP. The Vanderbilt University Center in Molecular Toxicology: the first 30 years. Drug Metab. Rev. 1999 Feb; 31(1): 1-11. PMID: 10065362, DOI: 10.1081/DMR-100101904, ISSN: 0360-2532.

Guengerich FP, Langouët S, Mican AN, Akasaka S, Müller M, Persmark M. Formation of etheno adducts and their effects on DNA polymerases. IARC Sci. Publ. 1999; (150): 137-45. PMID: 10626215, ISSN: 0300-5038.

Guengerich FP. Cytochrome P-450 3A4: regulation and role in drug metabolism. Annu. Rev. Pharmacol. Toxicol. 1999; 39: 1-17. PMID: 10331074, DOI: 10.1146/annurev.pharmtox.39.1.1, ISSN: 0362-1642.

Guengerich FP, Hosea NA, Parikh A, Bell-Parikh LC, Johnson WW, Gillam EM, Shimada T. Twenty years of biochemistry of human P450s: purification, expression, mechanism, and relevance to drugs. Drug Metab. Dispos. 1998 Dec; 26(12): 1175-8. PMID: 9860923, ISSN: 0090-9556.

Einolf HJ, Schnetz-Boutaud N, Guengerich FP. Steady-state and pre-steady-state kinetic analysis of 8-oxo-7,8-dihydroguanosine triphosphate incorporation and extension by replicative and repair DNA polymerases. Biochemistry. 1998 Sep 9/22/1998; 37(38): 13300-12. PMID: 9748338, PII: bi981346d, DOI: 10.1021/bi981346d, ISSN: 0006-2960.

Shimada T, Yamazaki H, Foroozesh M, Hopkins NE, Alworth WL, Guengerich FP. Selectivity of polycyclic inhibitors for human cytochrome P450s 1A1, 1A2, and 1B1. Chem. Res. Toxicol. 1998 Sep; 11(9): 1048-56. PMID: 9760279, PII: tx980090+, DOI: 10.1021/tx980090+, ISSN: 0893-228X.

Shimada T, Wunsch RM, Hanna IH, Sutter TR, Guengerich FP, Gillam EM. Recombinant human cytochrome P450 1B1 expression in Escherichia coli. Arch. Biochem. Biophys. 1998 Sep 9/1/1998; 357(1): 111-20. PMID: 9721189, PII: S0003-9861(98)90808-6, DOI: 10.1006/abbi.1998.0808, ISSN: 0003-9861.

Guengerich FP. The Environmental Genome Project: functional analysis of polymorphisms. Environ. Health Perspect. 1998 Jul; 106(7): 365-8. PMID: 9637792, PMCID: PMC1533131, ISSN: 0091-6765.

Guengerich FP, Johnson WW, Shimada T, Ueng YF, Yamazaki H, Langouët S. Activation and detoxication of aflatoxin B1. Mutat. Res. 1998 Jun 6/18/1998; 402(1-2): 121-8. PMID: 9675258, PII: S0027-5107(97)00289-3, ISSN: 0027-5107.

Guengerich FP, Shimada T. Activation of procarcinogens by human cytochrome P450 enzymes. Mutat. Res. 1998 May 5/25/1998; 400(1-2): 201-13. PMID: 9685642, ISSN: 0027-5107.

Hosea NA, Guengerich FP. Oxidation of nonionic detergents by cytochrome P450 enzymes. Arch. Biochem. Biophys. 1998 May 5/15/1998; 353(2): 365-73. PMID: 9606971, PII: S0003-9861(98)90659-2, DOI: 10.1006/abbi.1998.0659, ISSN: 0003-9861.

Langouët S, Mican AN, Müller M, Fink SP, Marnett LJ, Muhle SA, Guengerich FP. Misincorporation of nucleotides opposite five-membered exocyclic ring guanine derivatives by escherichia coli polymerases in vitro and in vivo: 1,N2-ethenoguanine, 5,6,7,9-tetrahydro-9-oxoimidazo[1, 2-a]purine, and 5,6,7,9-tetrahydro-7-hydroxy-9-oxoimidazo[1, 2-a]purine. Biochemistry. 1998 Apr 4/14/1998; 37(15): 5184-93. PMID: 9548749, PII: bi972327r, DOI: 10.1021/bi972327r, ISSN: 0006-2960.

Kim MS, Guengerich FP. Polymerase blockage and misincorporation of dNTPs opposite the ethylene dibromide-derived DNA adducts S-[2-(N7-guanyl)ethyl]glutathione, S-[2-(N2-guanyl)ethyl]glutathione, and S-[2-(O6-guanyl)ethyl]glutathione. Chem. Res. Toxicol. 1998 Apr; 11(4): 311-6. PMID: 9548801, PII: tx970206m, DOI: 10.1021/tx970206m, ISSN: 0893-228X.

Furge LL, Guengerich FP. Pre-steady-state kinetics of nucleotide insertion following 8-oxo-7,8-dihydroguanine base pair mismatches by bacteriophage T7 DNA polymerase exo-. Biochemistry. 1998 Mar 3/10/1998; 37(10): 3567-74. PMID: 9521678, PII: bi9722094, DOI: 10.1021/bi9722094, ISSN: 0006-2960.

Parikh A, Guengerich FP. Random mutagenesis by whole-plasmid PCR amplification. BioTechniques. 1998 Mar; 24(3): 428-31. PMID: 9526653, ISSN: 0736-6205.

Guengerich FP, Hosea NA, Martin MV. Purification of cytochromes P450: products of bacterial recombinant expression systems. Methods Mol. Biol. 1998; 107: 77-83. PMID: 14577214, DOI: 10.1385/0-89603-519-0:77, ISSN: 1064-3745.

Guengerich FP, Martin MV. Purification of cytochromes P450. Rat and human hepatic forms. Methods Mol. Biol. 1998; 107: 35-53. PMID: 14577211, DOI: 10.1385/0-89603-519-0:35, ISSN: 1064-3745.

Guengerich FP, Johnson WW. Kinetics of ferric cytochrome P450 reduction by NADPH-cytochrome P450 reductase: rapid reduction in the absence of substrate and variations among cytochrome P450 systems. Biochemistry. 1997 Dec 12/2/1997; 36(48): 14741-50. PMID: 9398194, PII: bi9719399, DOI: 10.1021/bi9719399, ISSN: 0006-2960.

Bell LC, Guengerich FP. Oxidation kinetics of ethanol by human cytochrome P450 2E1. Rate-limiting product release accounts for effects of isotopic hydrogen substitution and cytochrome b5 on steady-state kinetics. J. Biol. Chem. 1997 Nov 11/21/1997; 272(47): 29643-51. PMID: 9368031, ISSN: 0021-9258.

Guengerich FP, Parikh A, Johnson EF, Richardson TH, von Wachenfeldt C, Cosme J, Jung F, Strassburg CP, Manns MP, Tukey RH, Pritchard M, Fournel-Gigleux S, Burchell B. Heterologous expression of human drug-metabolizing enzymes. Drug Metab. Dispos. 1997 Nov; 25(11): 1234-41. PMID: 9351898, ISSN: 0090-9556.

Guengerich FP. Comparisons of catalytic selectivity of cytochrome P450 subfamily enzymes from different species. Chem. Biol. Interact. 1997 Oct 10/24/1997; 106(3): 161-82. PMID: 9413544, PII: S0009-2797(97)00068-9, ISSN: 0009-2797.

Kim MS, Guengerich FP. Synthesis of oligonucleotides containing the ethylene dibromide-derived DNA adducts S-[2-(N7-guanyl)ethyl]glutathione, S-[2-(N2-guanyl)ethyl]glutathione, and S-[2-(O6-guanyl)ethyl]glutathione at a single site. Chem. Res. Toxicol. 1997 Oct; 10(10): 1133-43. PMID: 9348436, PII: tx9701081, DOI: 10.1021/tx9701081, ISSN: 0893-228X.

Guengerich FP, Parikh A. Expression of drug-metabolizing enzymes. Curr. Opin. Biotechnol. 1997 Oct; 8(5): 623-8. PMID: 9353222, PII: S0958-1669(97)80039-0, ISSN: 0958-1669.

Parikh A, Gillam EM, Guengerich FP. Drug metabolism by Escherichia coli expressing human cytochromes P450. Nat. Biotechnol. 1997 Aug; 15(8): 784-8. PMID: 9255795, DOI: 10.1038/nbt0897-784, ISSN: 1087-0156.

Johnson WW, Guengerich FP. Reaction of aflatoxin B1 exo-8,9-epoxide with DNA: kinetic analysis of covalent binding and DNA-induced hydrolysis. Proc. Natl. Acad. Sci. U.S.A. 1997 Jun 6/10/1997; 94(12): 6121-5. PMID: 9177180, PMCID: PMC21012, ISSN: 0027-8424.

Johnson WW, Yamazaki H, Shimada T, Ueng YF, Guengerich FP. Aflatoxin B1 8,9-epoxide hydrolysis in the presence of rat and human epoxide hydrolase. Chem. Res. Toxicol. 1997 Jun; 10(6): 672-6. PMID: 9208174, PII: tx960209j, DOI: 10.1021/tx960209j, ISSN: 0893-228X.

Furge LL, Guengerich FP. Analysis of nucleotide insertion and extension at 8-oxo-7,8-dihydroguanine by replicative T7 polymerase exo- and human immunodeficiency virus-1 reverse transcriptase using steady-state and pre-steady-state kinetics. Biochemistry. 1997 May 5/27/1997; 36(21): 6475-87. PMID: 9174365, PII: bi9627267, DOI: 10.1021/bi9627267, ISSN: 0006-2960.

Langouët S, Müller M, Guengerich FP. Misincorporation of dNTPs opposite 1,N2-ethenoguanine and 5,6,7,9-tetrahydro-7-hydroxy-9-oxoimidazo[1,2-a]purine in oligonucleotides by Escherichia coli polymerases I exo- and II exo-, T7 polymerase exo-, human immunodeficiency virus-1 reverse transcriptase, and rat polymerase beta. Biochemistry. 1997 May 5/20/1997; 36(20): 6069-79. PMID: 9166777, PII: bi962526v, DOI: 10.1021/bi962526v, ISSN: 0006-2960.

Parikh A, Guengerich FP. Expression, purification, and characterization of a catalytically active human cytochrome P450 1A2:rat NADPH-cytochrome P450 reductase fusion protein. Protein Expr. Purif. 1997 Apr; 9(3): 346-54. PMID: 9126606, PII: S1046-5928(97)90721-X, DOI: 10.1006/prep.1997.0721, ISSN: 1046-5928.

Johnson WW, Ueng YF, Widersten M, Mannervik B, Hayes JD, Sherratt PJ, Ketterer B, Guengerich FP. Conjugation of highly reactive aflatoxin B1 exo-8,9-epoxide catalyzed by rat and human glutathione transferases: estimation of kinetic parameters. Biochemistry. 1997 Mar 3/18/1997; 36(11): 3056-60. PMID: 9115980, PII: bi962537o, DOI: 10.1021/bi962537o, ISSN: 0006-2960.

Müller M, Belas FJ, Blair IA, Guengerich FP. Analysis of 1,N2-ethenoguanine and 5,6,7,9-tetrahydro-7-hydroxy-9-oxoimidazo[1,2-a]purine in DNA treated with 2-chlorooxirane by high performance liquid chromatography/electrospray mass spectrometry and comparison of amounts to other DNA adducts. Chem. Res. Toxicol. 1997 Feb; 10(2): 242-7. PMID: 9049437, PII: tx960124i, DOI: 10.1021/tx960124i, ISSN: 0893-228X.

Ueng YF, Kuwabara T, Chun YJ, Guengerich FP. Cooperativity in oxidations catalyzed by cytochrome P450 3A4. Biochemistry. 1997 Jan 1/14/1997; 36(2): 370-81. PMID: 9003190, PII: bi962359z, DOI: 10.1021/bi962359z, ISSN: 0006-2960.

Tateishi T, Soucek P, Caraco Y, Guengerich FP, Wood AJ. Colchicine biotransformation by human liver microsomes. Identification of CYP3A4 as the major isoform responsible for colchicine demethylation. Biochem. Pharmacol. 1997 Jan 1/10/1997; 53(1): 111-6. PMID: 8960070, PII: S0006295296006934, ISSN: 0006-2952.

Guengerich FP. Role of cytochrome P450 enzymes in drug-drug interactions. Adv. Pharmacol. 1997; 43: 7-35. PMID: 9342171, ISSN: 1054-3589.

Guengerich FP, Vaz AD, Raner GN, Pernecky SJ, Coon MJ. Evidence for a role of a perferryl-oxygen complex, FeO3+, in the N-oxygenation of amines by cytochrome P450 enzymes. Mol. Pharmacol. 1997 Jan; 51(1): 147-51. PMID: 9016357, ISSN: 0026-895X.

Guengerich FP, Kim MS, Müller M, Lowe LG. Chemical mechanisms of formation of DNA-carcinogen adducts, elucidation of potential of adducts for mutagenicity, and mechanisms of polymerase fidelity and mutation in the presence of adducts. Recent Results Cancer Res. 1997; 143: 49-63. PMID: 8912411, ISSN: 0080-0015.

Yamazaki H, Johnson WW, Ueng YF, Shimada T, Guengerich FP. Lack of electron transfer from cytochrome b5 in stimulation of catalytic activities of cytochrome P450 3A4. Characterization of a reconstituted cytochrome P450 3A4/NADPH-cytochrome P450 reductase system and studies with apo-cytochrome b5. J. Biol. Chem. 1996 Nov 11/1/1996; 271(44): 27438-44. PMID: 8910324, ISSN: 0021-9258.

Guengerich FP, Yun CH, Macdonald TL. Evidence for a 1-electron oxidation mechanism in N-dealkylation of N,N-dialkylanilines by cytochrome P450 2B1. Kinetic hydrogen isotope effects, linear free energy relationships, comparisons with horseradish peroxidase, and studies with oxygen surrogates. J. Biol. Chem. 1996 Nov 11/1/1996; 271(44): 27321-9. PMID: 8910308, ISSN: 0021-9258.

Guengerich FP. In vitro techniques for studying drug metabolism. J Pharmacokinet Biopharm. 1996 Oct; 24(5): 521-33. PMID: 9131489, ISSN: 0090-466X.

Müller M, Hutchinson LK, Guengerich FP. Addition of deoxyribose to guanine and modified DNA based by Lactobacillus helveticus trans-N-deoxyribosylase. Chem. Res. Toxicol. 1996 Oct; 9(7): 1140-4. PMID: 8902269, PII: tx9600661, DOI: 10.1021/tx9600661, ISSN: 0893-228X.

Kim RB, Yamazaki H, Chiba K, O'Shea D, Mimura M, Guengerich FP, Ishizaki T, Shimada T, Wilkinson GR. In vivo and in vitro characterization of CYP2E1 activity in Japanese and Caucasians. J. Pharmacol. Exp. Ther. 1996 Oct; 279(1): 4-11. PMID: 8858968, ISSN: 0022-3565.

Guengerich FP, Gillam EM, Shimada T. New applications of bacterial systems to problems in toxicology. Crit. Rev. Toxicol. 1996 Sep; 26(5): 551-83. PMID: 8891430, DOI: 10.3109/10408449609037477, ISSN: 1040-8444.

Dong MS, Bell LC, Guo Z, Phillips DR, Blair IA, Guengerich FP. Identification of retained N-formylmethionine in bacterial recombinant mammalian cytochrome P450 proteins with the N-terminal sequence MALLLAVFL...: roles of residues 3-5 in retention and membrane topology. Biochemistry. 1996 Aug 8/6/1996; 35(31): 10031-40. PMID: 8756465, PII: bi960873z, DOI: 10.1021/bi960873z, ISSN: 0006-2960.

Lowe LG, Guengerich FP. Steady-state and pre-steady-state kinetic analysis of dNTP insertion opposite 8-oxo-7,8-dihydroguanine by Escherichia coli polymerases I exo- and II exo-. Biochemistry. 1996 Jul 7/30/1996; 35(30): 9840-9. PMID: 8703958, PII: bi960485x, DOI: 10.1021/bi960485x, ISSN: 0006-2960.

Caraco Y, Tateishi T, Guengerich FP, Wood AJ. Microsomal codeine N-demethylation: cosegregation with cytochrome P4503A4 activity. Drug Metab. Dispos. 1996 Jul; 24(7): 761-4. PMID: 8818573, ISSN: 0090-9556.

Shimada T, Hayes CL, Yamazaki H, Amin S, Hecht SS, Guengerich FP, Sutter TR. Activation of chemically diverse procarcinogens by human cytochrome P-450 1B1. Cancer Res. 1996 Jul 7/1/1996; 56(13): 2979-84. PMID: 8674051, ISSN: 0008-5472.

Chun YJ, Shimada T, Guengerich FP. Construction of a human cytochrome P450 1A1: rat NADPH-cytochrome P450 reductase fusion protein cDNA and expression in Escherichia coli, purification, and catalytic properties of the enzyme in bacterial cells and after purification. Arch. Biochem. Biophys. 1996 Jun 6/1/1996; 330(1): 48-58. PMID: 8651703, PII: S0003-9861(96)90224-6, DOI: 10.1006/abbi.1996.0224, ISSN: 0003-9861.

Guengerich FP, Johnson WW, Ueng YF, Yamazaki H, Shimada T. Involvement of cytochrome P450, glutathione S-transferase, and epoxide hydrolase in the metabolism of aflatoxin B1 and relevance to risk of human liver cancer. Environ. Health Perspect. 1996 May; 104 Suppl 3: 557-62. PMID: 8781383, PMCID: PMC1469621, ISSN: 0091-6765.

Dong MS, Yamazaki H, Guo Z, Guengerich FP. Recombinant human cytochrome P450 1A2 and an N-terminal-truncated form: construction, purification, aggregation properties, and interactions with flavodoxin, ferredoxin, and NADPH-cytochrome P450 reductase. Arch. Biochem. Biophys. 1996 Mar 3/1/1996; 327(1): 11-9. PMID: 8615680, PII: S0003-9861(96)90086-7, DOI: 10.1006/abbi.1996.0086, ISSN: 0003-9861.

Anzenbacher P, Niwa T, Tolbert LM, Sirimanne SR, Guengerich FP. Oxidation of 9-alkylanthracenes by cytochrome P450 2B1, horseradish peroxidase, and iron tetraphenylporphine/iodosylbenzene systems: anaerobic and aerobic mechanisms. Biochemistry. 1996 Feb 2/27/1996; 35(8): 2512-20. PMID: 8611554, PII: bi952330f, DOI: 10.1021/bi952330f, ISSN: 0006-2960.

Chang MS, Boeglin WE, Guengerich FP, Brash AR. Cytochrome P450-dependent transformations of 15R- and 15S-hydroperoxyeicosatetraenoic acids: stereoselective formation of epoxy alcohol products. Biochemistry. 1996 Jan 1/16/1996; 35(2): 464-71. PMID: 8555216, PII: bi952081v, DOI: 10.1021/bi952081v, ISSN: 0006-2960.

Müller M, Belas F, Ueno H, Guengerich FP. Development of a mass spectrometric assay for 5,6,7,9-tetrahydro-7-hydroxy-9-oximidazo[1,2-alpha] purine in DNA modified by 2-chloro-oxirane. Adv. Exp. Med. Biol. 1996; 387: 31-6. PMID: 8794191, ISSN: 0065-2598.

Guengerich FP, Martin MV, Guo Z, Chun YJ. Purification of functional recombinant P450s from bacteria. Meth. Enzymol. 1996; 272: 35-44. PMID: 8791760, PII: S0076-6879(96)72006-2, ISSN: 0076-6879.

Tateishi T, Krivoruk Y, Ueng YF, Wood AJ, Guengerich FP, Wood M. Identification of human liver cytochrome P-450 3A4 as the enzyme responsible for fentanyl and sufentanil N-dealkylation. Anesth. Analg. 1996 Jan; 82(1): 167-72. PMID: 8712396, ISSN: 0003-2999.

Lang CC, Brown RM, Kinirons MT, Deathridge MA, Guengerich FP, Kelleher D, O'Briain DS, Ghishan FK, Wood AJ. Decreased intestinal CYP3A in celiac disease: reversal after successful gluten-free diet: a potential source of interindividual variability in first-pass drug metabolism. Clin. Pharmacol. Ther. 1996 Jan; 59(1): 41-6. PMID: 8549032, PII: S0009-9236(96)90022-3, DOI: 10.1016/S0009-9236(96)90022-3, ISSN: 0009-9236.

Soucek P, Martin MV, Ueng YF, Guengerich FP. Identification of a common cytochrome P450 epitope near the conserved heme-binding petide with antibodies raised against recombinant cytochrome P450 family 2 proteins. Biochemistry. 1995 Dec 12/12/1995; 34(49): 16013-21. PMID: 8519757, ISSN: 0006-2960.

Tateishi T, Wood AJ, Guengerich FP, Wood M. Biotransformation of tritiated fentanyl in human liver microsomes. Monitoring metabolism using phenylacetic acid and 2-phenylethanol. Biochem. Pharmacol. 1995 Nov 11/27/1995; 50(11): 1921-4. PMID: 8615873, PII: 0006-2952(95)02088-8, ISSN: 0006-2952.

Yamazaki H, Ueng YF, Shimada T, Guengerich FP. Roles of divalent metal ions in oxidations catalyzed by recombinant cytochrome P450 3A4 and replacement of NADPH--cytochrome P450 reductase with other flavoproteins, ferredoxin, and oxygen surrogates. Biochemistry. 1995 Jul 7/4/1995; 34(26): 8380-9. PMID: 7599128, ISSN: 0006-2960.

Guengerich FP, Okazaki O, Seto Y, Macdonald TL. Radical cation intermediates in N-dealkylation reactions. Xenobiotica. 1995 Jul; 25(7): 689-709. PMID: 7483667, DOI: 10.3109/00498259509061886, ISSN: 0049-8254.

Guengerich FP. Cytochrome P450 proteins and potential utilization in biodegradation. Environ. Health Perspect. 1995 Jun; 103 Suppl 5: 25-8. PMID: 8565903, PMCID: PMC1519292, ISSN: 0091-6765.

Thier R, Müller M, Taylor JB, Pemble SE, Ketterer B, Guengerich FP. Enhancement of bacterial mutagenicity of bifunctional alkylating agents by expression of mammalian glutathione S-transferase. Chem. Res. Toxicol. 1995 Apr; 8(3): 465-72. PMID: 7578934, ISSN: 0893-228X.

Guengerich FP. Influence of nutrients and other dietary materials on cytochrome P-450 enzymes. Am. J. Clin. Nutr. 1995 Mar; 61(3 Suppl): 651S-658S. PMID: 7879733, ISSN: 0002-9165.

Ueng YF, Shimada T, Yamazaki H, Guengerich FP. Oxidation of aflatoxin B1 by bacterial recombinant human cytochrome P450 enzymes. Chem. Res. Toxicol. 1995 Mar; 8(2): 218-25. PMID: 7766804, ISSN: 0893-228X.

Yamazaki H, Guo Z, Guengerich FP. Selectivity of cytochrome P4502E1 in chlorzoxazone 6-hydroxylation. Drug Metab. Dispos. 1995 Mar; 23(3): 438-40. PMID: 7628313, ISSN: 0090-9556.

Guengerich FP, Humphreys WG, Yun CH, Hammons GJ, Kadlubar FF, Seto Y, Okazaki O, Martin MV. Mechanisms of cytochrome P450 1A2-mediated formation of N-hydroxy arylamines and heterocyclic amines and their reaction with guanyl residues. Int. Symp. Princess Takamatsu Cancer Res. Fund. 1995; 23: 78-84. PMID: 8844798.

Guengerich FP, Bell LC, Okazaki O. Interpretations of cytochrome P450 mechanisms from kinetic studies. Biochimie. 1995; 77(7-8): 573-80. PMID: 8589068, PII: 0300-9084(96)88173-9, ISSN: 0300-9084.

Bauer E, Guo Z, Ueng YF, Bell LC, Zeldin D, Guengerich FP. Oxidation of benzo[a]pyrene by recombinant human cytochrome P450 enzymes. Chem. Res. Toxicol. 1995 Jan; 8(1): 136-42. PMID: 7703357, ISSN: 0893-228X.

Guengerich FP, Thier R, Persmark M, Taylor JB, Pemble SE, Ketterer B. Conjugation of carcinogens by theta class glutathione s-transferases: mechanisms and relevance to variations in human risk. Pharmacogenetics. 1995; 5 Spec No: S103-7. PMID: 7581478, ISSN: 0960-314X.

Shimada T, Gillam EM, Sandhu P, Guo Z, Tukey RH, Guengerich FP. Activation of procarcinogens by human cytochrome P450 enzymes expressed in Escherichia coli. Simplified bacterial systems for genotoxicity assays. Carcinogenesis. 1994 Nov; 15(11): 2523-9. PMID: 7955101, ISSN: 0143-3334.

Guengerich FP, Shimada T, Yun CH, Yamazaki H, Raney KD, Thier R, Coles B, Harris TM. Interactions of ingested food, beverage, and tobacco components involving human cytochrome P4501A2, 2A6, 2E1, and 3A4 enzymes. Environ. Health Perspect. 1994 Nov; 102 Suppl 9: 49-53. PMID: 7698084, PMCID: PMC1566791, ISSN: 0091-6765.

Guo Z, Gillam EM, Ohmori S, Tukey RH, Guengerich FP. Expression of modified human cytochrome P450 1A1 in Escherichia coli: effects of 5' substitution, stabilization, purification, spectral characterization, and catalytic properties. Arch. Biochem. Biophys. 1994 Aug 8/1/1994; 312(2): 436-46. PMID: 8037457, PII: S0003986184713300, ISSN: 0003-9861.

Persmark M, Guengerich FP. Spectroscopic and thermodynamic characterization of the interaction of N7-guanyl thioether derivatives of d(TGCTG*CAAG) with potential complements. Biochemistry. 1994 Jul 7/26/1994; 33(29): 8662-72. PMID: 8038155, ISSN: 0006-2960.

Gillam EM, Guo Z, Guengerich FP. Expression of modified human cytochrome P450 2E1 in Escherichia coli, purification, and spectral and catalytic properties. Arch. Biochem. Biophys. 1994 Jul; 312(1): 59-66. PMID: 8031147, PII: S000398618471280X, ISSN: 0003-9861.

Guengerich FP, Persmark M. Mechanism of formation of ethenoguanine adducts from 2-haloacetaldehydes: 13C-labeling patterns with 2-bromoacetaldehyde. Chem. Res. Toxicol. 1994 Mar; 7(2): 205-8. PMID: 8199310, ISSN: 0893-228X.

Sandhu P, Guo Z, Baba T, Martin MV, Tukey RH, Guengerich FP. Expression of modified human cytochrome P450 1A2 in Escherichia coli: stabilization, purification, spectral characterization, and catalytic activities of the enzyme. Arch. Biochem. Biophys. 1994 Feb 2/15/1994; 309(1): 168-77. PMID: 8117105, PII: S000398618471099X, ISSN: 0003-9861.

Guengerich FP. Catalytic selectivity of human cytochrome P450 enzymes: relevance to drug metabolism and toxicity. Toxicol. Lett. 1994 Feb 2/1/1994; 70(2): 133-8. PMID: 8296317, ISSN: 0378-4274.

Guengerich FP. Mechanisms of formation of DNA adducts from ethylene dihalides, vinyl halides, and arylamines. Drug Metab. Rev. 1994; 26(1-2): 47-66. PMID: 8082581, DOI: 10.3109/03602539409029784, ISSN: 0360-2532.

Guengerich FP. Metabolism and genotoxicity of dihaloalkanes. Adv. Pharmacol. 1994; 27: 211-36. PMID: 8068554, ISSN: 1054-3589.

Guengerich FP, Min KS, Persmark M, Kim MS, Humphreys WG, Cmarik JM, Thier R. Dihaloalkanes and polyhaloalkenes. IARC Sci. Publ. 1994; (125): 57-72. PMID: 7806341, ISSN: 0300-5038.

Persmark M, Humphreys WG, Okazaki O, Raney VM, Guengerich FP. Mechanisms of formation of 1,N6-ethenoadenine, 3,N4-ethenocytosine, 1,N2-ethenoguanine and N2,3-ethenoguanine DNA adducts from substituted oxiranes. IARC Sci. Publ. 1994; (125): 437-41. PMID: 7806335, ISSN: 0300-5038.

Sandhu P, Baba T, Guengerich FP. Expression of modified cytochrome P450 2C10 (2C9) in Escherichia coli, purification, and reconstitution of catalytic activity. Arch. Biochem. Biophys. 1993 Nov 11/1/1993; 306(2): 443-50. PMID: 8215449, PII: S0003-9861(83)71536-5, DOI: 10.1006/abbi.1993.1536, ISSN: 0003-9861.

Kim MS, Guengerich FP. Interactions of N7-guanyl methyl- and thioether-substituted d(CATGCCT) derivatives with d(AGGNATG). Chem. Res. Toxicol. 1993 Nov; 6(6): 900-5. PMID: 8117931, ISSN: 0893-228X.

Guengerich FP, Gillam EM, Ohmori S, Sandhu P, Brian WR, Sari MA, Iwasaki M. Expression of human cytochrome P450 enzymes in yeast and bacteria and relevance to studies on catalytic specificity. Toxicology. 1993 Oct 10/5/1993; 82(1-3): 21-37. PMID: 8236276, ISSN: 0300-483X.

Thier R, Taylor JB, Pemble SE, Humphreys WG, Persmark M, Ketterer B, Guengerich FP. Expression of mammalian glutathione S-transferase 5-5 in Salmonella typhimurium TA1535 leads to base-pair mutations upon exposure to dihalomethanes. Proc. Natl. Acad. Sci. U.S.A. 1993 Sep 9/15/1993; 90(18): 8576-80. PMID: 8378332, PMCID: PMC47400, ISSN: 0027-8424.

Seto Y, Guengerich FP. Liquid chromatographic determination of para-substituted N,N-dialkylaniline N-oxides. J. Chromatogr. 1993 Sep 9/8/1993; 619(1): 71-7. PMID: 8245165.

Guengerich FP, Persmark M, Humphreys WG. Formation of 1,N2- and N2,3-ethenoguanine from 2-halooxiranes: isotopic labeling studies and isolation of a hemiaminal derivative of N2-(2-oxoethyl)guanine. Chem. Res. Toxicol. 1993 Sep; 6(5): 635-48. PMID: 8292741, ISSN: 0893-228X.

Gillam EM, Baba T, Kim BR, Ohmori S, Guengerich FP. Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme. Arch. Biochem. Biophys. 1993 Aug 8/15/1993; 305(1): 123-31. PMID: 8342945, PII: S0003-9861(83)71401-3, DOI: 10.1006/abbi.1993.1401, ISSN: 0003-9861.

Karara A, Makita K, Jacobson HR, Falck JR, Guengerich FP, DuBois RN, Capdevila JH. Molecular cloning, expression, and enzymatic characterization of the rat kidney cytochrome P-450 arachidonic acid epoxygenase. J. Biol. Chem. 1993 Jun 6/25/1993; 268(18): 13565-70. PMID: 8514789, ISSN: 0021-9258.

Seto Y, Guengerich FP. Partitioning between N-dealkylation and N-oxygenation in the oxidation of N,N-dialkylarylamines catalyzed by cytochrome P450 2B1. J. Biol. Chem. 1993 May 5/15/1993; 268(14): 9986-97. PMID: 8486725, ISSN: 0021-9258.

Yun CH, Okerholm RA, Guengerich FP. Oxidation of the antihistaminic drug terfenadine in human liver microsomes. Role of cytochrome P-450 3A(4) in N-dealkylation and C-hydroxylation. Drug Metab. Dispos. 1993 May; 21(3): 403-9. PMID: 8100494, ISSN: 0090-9556.

Okazaki O, Persmark M, Guengerich FP. N-nitroso-N-methylvinylamine: reaction of the epoxide with guanyl and adenyl moieties to yield adducts derived from both parts of the molecule. Chem. Res. Toxicol. 1993 Mar; 6(2): 168-73. PMID: 8477007, ISSN: 0893-228X.

Okazaki O, Guengerich FP. Evidence for specific base catalysis in N-dealkylation reactions catalyzed by cytochrome P450 and chloroperoxidase. Differences in rates of deprotonation of aminium radicals as an explanation for high kinetic hydrogen isotope effects observed with peroxidases. J. Biol. Chem. 1993 Jan 1/25/1993; 268(3): 1546-52. PMID: 8380572, ISSN: 0021-9258.

Guengerich FP. The 1992 Bernard B. Brodie Award Lecture. Bioactivation and detoxication of toxic and carcinogenic chemicals. Drug Metab. Dispos. 1993 Jan; 21(1): 1-6. PMID: 8095200, ISSN: 0090-9556.

Yun CH, Hammons GJ, Jones G, Martin MV, Hopkins NE, Alworth WL, Guengerich FP. Modification of cytochrome P450 1A2 enzymes by the mechanism-based inactivator 2-ethynylnaphthalene and the photoaffinity label 4-azidobiphenyl. Biochemistry. 1992 Nov 11/3/1992; 31(43): 10556-63. PMID: 1420171, ISSN: 0006-2960.

Guengerich FP, Shimada T, Raney KD, Yun CH, Meyer DJ, Ketterer B, Harris TM, Groopman JD, Kadlubar FF. Elucidation of catalytic specificities of human cytochrome P450 and glutathione S-transferase enzymes and relevance to molecular epidemiology. Environ. Health Perspect. 1992 Nov; 98: 75-80. PMID: 1486866, PMCID: PMC1519604, ISSN: 0091-6765.

Capdevila JH, Wei S, Yan J, Karara A, Jacobson HR, Falck JR, Guengerich FP, DuBois RN. Cytochrome P-450 arachidonic acid epoxygenase. Regulatory control of the renal epoxygenase by dietary salt loading. J. Biol. Chem. 1992 Oct 10/25/1992; 267(30): 21720-6. PMID: 1400482, ISSN: 0021-9258.

Yun CH, Wood M, Wood AJ, Guengerich FP. Identification of the pharmacogenetic determinants of alfentanil metabolism: cytochrome P-450 3A4. An explanation of the variable elimination clearance. Anesthesiology. 1992 Sep; 77(3): 467-74. PMID: 1519785, ISSN: 0003-3022.

Humphreys WG, Kadlubar FF, Guengerich FP. Mechanism of C8 alkylation of guanine residues by activated arylamines: evidence for initial adduct formation at the N7 position. Proc. Natl. Acad. Sci. U.S.A. 1992 Sep 9/1/1992; 89(17): 8278-82. PMID: 1518858, PMCID: PMC49901, ISSN: 0027-8424.

Raney KD, Meyer DJ, Ketterer B, Harris TM, Guengerich FP. Glutathione conjugation of aflatoxin B1 exo- and endo-epoxides by rat and human glutathione S-transferases. Chem. Res. Toxicol. 1992 Jul; 5(4): 470-8. PMID: 1391613, ISSN: 0893-228X.

Fleming CM, Branch RA, Wilkinson GR, Guengerich FP. Human liver microsomal N-hydroxylation of dapsone by cytochrome P-4503A4. Mol. Pharmacol. 1992 May; 41(5): 975-80. PMID: 1588928, ISSN: 0026-895X.

Shimada T, Yun CH, Yamazaki H, Gautier JC, Beaune PH, Guengerich FP. Characterization of human lung microsomal cytochrome P-450 1A1 and its role in the oxidation of chemical carcinogens. Mol. Pharmacol. 1992 May; 41(5): 856-64. PMID: 1588920, ISSN: 0026-895X.

Raney KD, Coles B, Guengerich FP, Harris TM. The endo-8,9-epoxide of aflatoxin B1: a new metabolite. Chem. Res. Toxicol. 1992 May; 5(3): 333-5. PMID: 1504254, ISSN: 0893-228X.

Cmarik JL, Humphreys WG, Bruner KL, Lloyd RS, Tibbetts C, Guengerich FP. Mutation spectrum and sequence alkylation selectivity resulting from modification of bacteriophage M13mp18 DNA with S-(2-chloroethyl)glutathione. Evidence for a role of S-(2-N7-guanyl)ethyl)glutathione as a mutagenic lesion formed from ethylene dibromide. J. Biol. Chem. 1992 Apr 4/5/1992; 267(10): 6672-9. PMID: 1551876, ISSN: 0021-9258.

Yun CH, Shimada T, Guengerich FP. Roles of human liver cytochrome P4502C and 3A enzymes in the 3-hydroxylation of benzo(a)pyrene. Cancer Res. 1992 Apr 4/1/1992; 52(7): 1868-74. PMID: 1551116, ISSN: 0008-5472.

Raney KD, Shimada T, Kim DH, Groopman JD, Harris TM, Guengerich FP. Oxidation of aflatoxins and sterigmatocystin by human liver microsomes: significance of aflatoxin Q1 as a detoxication product of aflatoxin B1. Chem. Res. Toxicol. 1992 Mar; 5(2): 202-10. PMID: 1643250, ISSN: 0893-228X.

Yun CH, Shimada T, Guengerich FP. Contributions of human liver cytochrome P450 enzymes to the N-oxidation of 4,4'-methylene-bis(2-chloroaniline). Carcinogenesis. 1992 Feb; 13(2): 217-22. PMID: 1740010, ISSN: 0143-3334.

Guengerich FP. Characterization of human cytochrome P450 enzymes. FASEB J. 1992 Jan 1/6/1992; 6(2): 745-8. PMID: 1537465, ISSN: 0892-6638.

Guengerich FP. Cytochrome P450: advances and prospects. FASEB J. 1992 Jan 1/6/1992; 6(2): 667-8. PMID: 1537453, ISSN: 0892-6638.

Guengerich FP, Raney KD, Kim DH, Shimada T, Meyer DJ, Ketterer B, Harris TM. Oxidation and conjugation of aflatoxins by humans and experimental animals. Prog. Clin. Biol. Res. 1992; 374: 157-65. PMID: 1620701, ISSN: 0361-7742.

Guengerich FP. Roles of the vinyl chloride oxidation products 1-chlorooxirane and 2-chloroacetaldehyde in the in vitro formation of etheno adducts of nucleic acid bases [corrected]. Chem. Res. Toxicol. 1992 Jan; 5(1): 2-5. PMID: 1581532, ISSN: 0893-228X.

Guengerich FP. Human cytochrome P-450 enzymes. Life Sci. 1992; 50(20): 1471-8. PMID: 1579042, ISSN: 0024-3205.

Guengerich FP. Metabolic activation of carcinogens. Pharmacol. Ther. 1992; 54(1): 17-61. PMID: 1528954, ISSN: 0163-7258.

Yun CH, Shimada T, Guengerich FP. Purification and characterization of human liver microsomal cytochrome P-450 2A6. Mol. Pharmacol. 1991 Nov; 40(5): 679-85. PMID: 1944238, ISSN: 0026-895X.

Humphreys WG, Guengerich FP. Structure of formamidopyrimidine adducts as determined by NMR using specifically 15N-labeled guanosine. Chem. Res. Toxicol. 1991 Nov; 4(6): 632-6. PMID: 1807445, ISSN: 0893-228X.

Oida T, Humphreys WG, Guengerich FP. Preparation and characterization of oligonucleotides containing S-[2-(N7-guanyl)ethyl]glutathione. Biochemistry. 1991 Oct 10/29/1991; 30(43): 10513-22. PMID: 1657147, ISSN: 0006-2960.

Shimada T, Guengerich FP. Activation of amino-alpha-carboline, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and a copper phthalocyanine cellulose extract of cigarette smoke condensate by cytochrome P-450 enzymes in rat and human liver microsomes. Cancer Res. 1991 Oct 10/1/1991; 51(19): 5284-91. PMID: 1913651, ISSN: 0008-5472.

Guengerich FP. Molecular advances for the cytochrome P-450 superfamily. Trends Pharmacol. Sci. 1991 Aug; 12(8): 281-3. PMID: 1949194, ISSN: 0165-6147.

Humphreys WG, Kim DH, Guengerich FP. Isolation and characterization of N7-guanyl adducts derived from 1,2-dibromo-3-chloropropane. Chem. Res. Toxicol. 1991 Jul; 4(4): 445-53. PMID: 1912332, ISSN: 0893-228X.

Guengerich FP, Kim DH. Enzymatic oxidation of ethyl carbamate to vinyl carbamate and its role as an intermediate in the formation of 1,N6-ethenoadenosine. Chem. Res. Toxicol. 1991 Jul; 4(4): 413-21. PMID: 1912327, ISSN: 0893-228X.

Guengerich FP, Shimada T. Oxidation of toxic and carcinogenic chemicals by human cytochrome P-450 enzymes. Chem. Res. Toxicol. 1991 Jul; 4(4): 391-407. PMID: 1912325, ISSN: 0893-228X.

Srivastava PK, Yun CH, Beaune PH, Ged C, Guengerich FP. Separation of human liver microsomal tolbutamide hydroxylase and (S)-mephenytoin 4'-hydroxylase cytochrome P-450 enzymes. Mol. Pharmacol. 1991 Jul; 40(1): 69-79. PMID: 1857342, ISSN: 0026-895X.

Guengerich FP. Reactions and significance of cytochrome P-450 enzymes. J. Biol. Chem. 1991 Jun 6/5/1991; 266(16): 10019-22. PMID: 2037557, ISSN: 0021-9258.

Guengerich FP, Brian WR, Iwasaki M, Sari MA, Bäärnhielm C, Berntsson P. Oxidation of dihydropyridine calcium channel blockers and analogues by human liver cytochrome P-450 IIIA4. J. Med. Chem. 1991 Jun; 34(6): 1838-44. PMID: 2061924, ISSN: 0022-2623.

Guengerich FP, Turvy CG. Comparison of levels of several human microsomal cytochrome P-450 enzymes and epoxide hydrolase in normal and disease states using immunochemical analysis of surgical liver samples. J. Pharmacol. Exp. Ther. 1991 Mar; 256(3): 1189-94. PMID: 2005581, ISSN: 0022-3565.

Guengerich FP, Kim DH, Iwasaki M. Role of human cytochrome P-450 IIE1 in the oxidation of many low molecular weight cancer suspects. Chem. Res. Toxicol. 1991 Mar; 4(2): 168-79. PMID: 1664256, ISSN: 0893-228X.

Guengerich FP, Shimada T, Bondon A, Macdonald TL. Cytochrome P-450 oxidations and the generation of biologically reactive intermediates. Adv. Exp. Med. Biol. 1991; 283: 1-11. PMID: 2068975, ISSN: 0065-2598.

Oida T, Humphreys WG, Guengerich FP. Effects of adduct formation derived from ethylene dibromide on DNA conformation. Nucleic Acids Symp. Ser. 1991; (25): 135-6. PMID: 1842056, ISSN: 0261-3166.

Brian WR, Sari MA, Iwasaki M, Shimada T, Kaminsky LS, Guengerich FP. Catalytic activities of human liver cytochrome P-450 IIIA4 expressed in Saccharomyces cerevisiae. Biochemistry. 1990 Dec 12/25/1990; 29(51): 11280-92. PMID: 2271712, ISSN: 0006-2960.

Guengerich FP, Kim DH. In vitro inhibition of dihydropyridine oxidation and aflatoxin B1 activation in human liver microsomes by naringenin and other flavonoids. Carcinogenesis. 1990 Dec; 11(12): 2275-9. PMID: 2265479, ISSN: 0143-3334.

Guengerich FP. Inhibition of oral contraceptive steroid-metabolizing enzymes by steroids and drugs. Am. J. Obstet. Gynecol. 1990 Dec; 163(6 Pt 2): 2159-63. PMID: 2256525, ISSN: 0002-9378.

Humphreys WG, Kim DH, Cmarik JL, Shimada T, Guengerich FP. Comparison of the DNA-alkylating properties and mutagenic responses of a series of S-(2-haloethyl)-substituted cysteine and glutathione derivatives. Biochemistry. 1990 Nov 11/13/1990; 29(45): 10342-50. PMID: 2261477, ISSN: 0006-2960.

Kim DH, Humphreys WG, Guengerich FP. Characterization of S-[2-(N1-adenyl)ethyl]glutathione as an adduct formed in RNA and DNA from 1,2-dibromoethane. Chem. Res. Toxicol. 1990 Nov; 3(6): 587-94. PMID: 1715767, ISSN: 0893-228X.

Guengerich FP. Mechanism-based inactivation of human liver microsomal cytochrome P-450 IIIA4 by gestodene. Chem. Res. Toxicol. 1990 Jul; 3(4): 363-71. PMID: 2133086, ISSN: 0893-228X.

Cmarik JL, Inskeep PB, Meredith MJ, Meyer DJ, Ketterer B, Guengerich FP. Selectivity of rat and human glutathione S-transferases in activation of ethylene dibromide by glutathione conjugation and DNA binding and induction of unscheduled DNA synthesis in human hepatocytes. Cancer Res. 1990 May 5/1/1990; 50(9): 2747-52. PMID: 2328501, ISSN: 0008-5472.

Guengerich FP, MacDonald TL. Mechanisms of cytochrome P-450 catalysis. FASEB J. 1990 May; 4(8): 2453-9. PMID: 2185971, ISSN: 0892-6638.

Shimada T, Guengerich FP. Inactivation of 1,3-, 1,6-, and 1,8-dinitropyrene by cytochrome P-450 enzymes in human and rat liver microsomes. Cancer Res. 1990 Apr 4/1/1990; 50(7): 2036-43. PMID: 2180560, ISSN: 0008-5472.

Kim DH, Guengerich FP. Formation of the DNA adduct S-[2-(N7-guanyl)ethyl]glutathione from ethylene dibromide: effects of modulation of glutathione and glutathione S-transferase levels and lack of a role for sulfation. Carcinogenesis. 1990 Mar; 11(3): 419-24. PMID: 2311185, ISSN: 0143-3334.

Guengerich FP. Purification and characterization of xenobiotic-metabolizing enzymes from lung tissue. Pharmacol. Ther. 1990; 45(3): 299-307. PMID: 2405438, ISSN: 0163-7258.

Guengerich FP, Shimada T, Iwasaki M, Butler MA, Kadlubar FF. Activation of carcinogens by human liver cytochromes P-450. Basic Life Sci. 1990; 53: 381-96. PMID: 2282045, ISSN: 0090-5542.

Guengerich FP. Metabolism of 17 alpha-ethynylestradiol in humans. Life Sci. 1990; 47(22): 1981-8. PMID: 2273938, ISSN: 0024-3205.

Guengerich FP, Shimada T, Iwasaki M, Martin MV. Activation of mutagens by human cytochrome P-450 enzymes. Prog. Clin. Biol. Res. 1990; 340B: 87-96. PMID: 2168051, ISSN: 0361-7742.

Guengerich FP, Humphreys WG, Kim DH, Oida T, Cmarik JL. DNA-glutathione adducts derived from vic-dihaloalkanes: mechanisms of mutagenesis. Int. Symp. Princess Takamatsu Cancer Res. Fund. 1990; 21: 101-7. PMID: 2134669.

Guengerich FP. Low kinetic hydrogen isotope effects in the dehydrogenation of 1,4-dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethyl ester (nifedipine) by cytochrome P-450 enzymes are consistent with an electron/proton/electron transfer mechanism. Chem. Res. Toxicol. 1990 Jan; 3(1): 21-6. PMID: 2131820, ISSN: 0893-228X.

Shimada T, Martin MV, Pruess-Schwartz D, Marnett LJ, Guengerich FP. Roles of individual human cytochrome P-450 enzymes in the bioactivation of benzo(a)pyrene, 7,8-dihydroxy-7,8-dihydrobenzo(a)pyrene, and other dihydrodiol derivatives of polycyclic aromatic hydrocarbons. Cancer Res. 1989 Nov 11/15/1989; 49(22): 6304-12. PMID: 2509067, ISSN: 0008-5472.

Kim DH, Guengerich FP. Excretion of the mercapturic acid S-[2-(N7-guanyl)ethyl]-N-acetylcysteine in urine following administration of ethylene dibromide to rats. Cancer Res. 1989 Nov 11/1/1989; 49(21): 5843-7. PMID: 2790795, ISSN: 0008-5472.

Guengerich FP. Oxidation of halogenated compounds by cytochrome P-450, peroxidases, and model metalloporphyrins. J. Biol. Chem. 1989 Oct 10/15/1989; 264(29): 17098-205. PMID: 2793846, ISSN: 0021-9258.

Shimada T, Iwasaki M, Martin MV, Guengerich FP. Human liver microsomal cytochrome P-450 enzymes involved in the bioactivation of procarcinogens detected by umu gene response in Salmonella typhimurium TA 1535/pSK1002. Cancer Res. 1989 Jun 6/15/1989; 49(12): 3218-28. PMID: 2655891, ISSN: 0008-5472.

Brian WR, Srivastava PK, Umbenhauer DR, Lloyd RS, Guengerich FP. Expression of a human liver cytochrome P-450 protein with tolbutamide hydroxylase activity in Saccharomyces cerevisiae. Biochemistry. 1989 Jun 6/13/1989; 28(12): 4993-9. PMID: 2669966, ISSN: 0006-2960.

Baertschi SW, Raney KD, Shimada T, Harris TM, Guengerich FP. Comparison of rates of enzymatic oxidation of aflatoxin B1, aflatoxin G1, and sterigmatocystin and activities of the epoxides in forming guanyl-N7 adducts and inducing different genetic responses. Chem. Res. Toxicol. 1989 Mar; 2(2): 114-2. PMID: 2519710, ISSN: 0893-228X.

Bondon A, Macdonald TL, Harris TM, Guengerich FP. Oxidation of cycloalkylamines by cytochrome P-450. Mechanism-based inactivation, adduct formation, ring expansion, and nitrone formation. J. Biol. Chem. 1989 Feb 2/5/1989; 264(4): 1988-97. PMID: 2914890, ISSN: 0021-9258.

Bork RW, Muto T, Beaune PH, Srivastava PK, Lloyd RS, Guengerich FP. Characterization of mRNA species related to human liver cytochrome P-450 nifedipine oxidase and the regulation of catalytic activity. J. Biol. Chem. 1989 Jan 1/15/1989; 264(2): 910-9. PMID: 2463251, ISSN: 0021-9258.

Brian WR, Ged C, Bellew TM, Srivastava PK, Bork RW, Umbenhauer DR, Lloyd RS, Guengerich FP. Human liver mephenytoin 4'-hydroxylase cytochrome P-450 proteins and genes. Drug Metab. Rev. 1989; 20(2-4): 449-65. PMID: 2680390, DOI: 10.3109/03602538909103552, ISSN: 0360-2532.

Wilkinson GR, Guengerich FP, Branch RA. Genetic polymorphism of S-mephenytoin hydroxylation. Pharmacol. Ther. 1989; 43(1): 53-76. PMID: 2675129, ISSN: 0163-7258.

Guengerich FP. Characterization of human microsomal cytochrome P-450 enzymes. Annu. Rev. Pharmacol. Toxicol. 1989; 29: 241-64. PMID: 2658771, DOI: 10.1146/annurev.pa.29.040189.001325, ISSN: 0362-1642.

Shimada T, Guengerich FP. Evidence for cytochrome P-450NF, the nifedipine oxidase, being the principal enzyme involved in the bioactivation of aflatoxins in human liver. Proc. Natl. Acad. Sci. U.S.A. 1989 Jan; 86(2): 462-5. PMID: 2492107, PMCID: PMC286490, ISSN: 0027-8424.

Ged C, Umbenhauer DR, Bellew TM, Bork RW, Srivastava PK, Shinriki N, Lloyd RS, Guengerich FP. Characterization of cDNAs, mRNAs, and proteins related to human liver microsomal cytochrome P-450 (S)-mephenytoin 4'-hydroxylase. Biochemistry. 1988 Sep 9/6/1988; 27(18): 6929-40. PMID: 3196692, ISSN: 0006-2960.

Guengerich FP, Peterson LA, Böcker RH. Cytochrome P-450-catalyzed hydroxylation and carboxylic acid ester cleavage of Hantzsch pyridine esters. J. Biol. Chem. 1988 Jun 6/15/1988; 263(17): 8176-83. PMID: 3372518, ISSN: 0021-9258.

Guengerich FP, Böcker RH. Cytochrome P-450-catalyzed dehydrogenation of 1,4-dihydropyridines. J. Biol. Chem. 1988 Jun 6/15/1988; 263(17): 8168-75. PMID: 3372517, ISSN: 0021-9258.

Guengerich FP. Roles of cytochrome P-450 enzymes in chemical carcinogenesis and cancer chemotherapy. Cancer Res. 1988 Jun 6/1/1988; 48(11): 2946-54. PMID: 3284639, ISSN: 0008-5472.

Knodell RG, Dubey RK, Wilkinson GR, Guengerich FP. Oxidative metabolism of hexobarbital in human liver: relationship to polymorphic S-mephenytoin 4-hydroxylation. J. Pharmacol. Exp. Ther. 1988 Jun; 245(3): 845-9. PMID: 3385644, ISSN: 0022-3565.

Guengerich FP. Oxidation of 17 alpha-ethynylestradiol by human liver cytochrome P-450. Mol. Pharmacol. 1988 May; 33(5): 500-8. PMID: 3285175, ISSN: 0026-895X.

Guengerich FP. Cytochromes P-450. Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol. 1988; 89(1): 1-4. PMID: 2894265, ISSN: 0742-8413.

Guengerich FP, Peterson LA, Cmarik JL, Koga N, Inskeep PB. Activation of dihaloalkanes by glutathione conjugation and formation of DNA adducts. Environ. Health Perspect. 1987 Dec; 76: 15-8. PMID: 3329096, PMCID: PMC1474475, ISSN: 0091-6765.

Guengerich FP, Churchill PF, Jung CY, Fleischer S. Target inactivation analysis applied to determination of molecular weights of rat liver proteins in the purified state and in microsomal membranes. Biochim. Biophys. Acta. 1987 Sep 9/24/1987; 915(2): 246-53. PMID: 3115294, ISSN: 0006-3002.

Guengerich FP, Beaune PH, Umbenhauer DR, Churchill PF, Bork RW, Dannan GA, Knodell RG, Lloyd RS, Martin MV. Cytochrome P-450 enzymes involved in genetic polymorphism of drug oxidation in humans. Biochem. Soc. Trans. 1987 Aug; 15(4): 576-8. PMID: 3678578, ISSN: 0300-5127.

Posted: 10/25/2010

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Please apply by e-mail to f.guengerich@vanderbilt.edu sending CV and names of three references.