Danyvid Olivares-Villagomez, Ph.D.

Assistant Professor

Faculty Appointments
Assistant Professor of Pathology, Microbiology and Immunology
Ph.D., PATHOLOGY, New York University, New York, New YorkM.S., Biology , Vanderbilt University, Nashville, TennesseeB.S., Universidad Nacional Autónoma de México, Mexico City, Mexico
Office Address
MCN A5211
21 Ave and Garland, 811 Light Hall. Nashville TN 37232
Nashville, TN 37232
Research Description
Our bodies are constantly exposed to infectious agents wanting to invade and proliferate within us. One of the areas with high exposure to foreign antigens is the mucosa of the digestive tract. The mucosal immune system protects the intestines from pathogenic invaders and at the same time discriminates those organisms that are important for our overall homeostasis. My research focuses on the role of intestinal intraepithelial lymphocytes, IEL (a group of immune cells present in the intestinal epithelium) during immune responses in the mucosa.

Our current research involves a novel IEL population discovered in our lab known as innate CD8alpha-alpha cells (or iCD8alpha). We have shown that this population of cells is important for controlling the colonization of pathogenic bacteria, such as Citrobacter rodentium. We believe that one of the roles of these cells is to provide survival signals to other IEL via the cytokine osteopontin. Moreover, we have also observed that iCD8alpha cells mediate mucosal immune responses through secreted granzymes.

These are exciting areas of research that once better understood will provide evidence of how mucosal immune responses occur in the intestinal epithelium, and will provide foundations for therapeutic interventions of gastrointestinal diseases.

Research Keywords
Mucosal immunology, IEL, iCD8alpha, osteopontin, granzyme
Hoek KL, Greer MJ, McClanahan KG, Nazmi A, Piazuelo MB, Singh K, Wilson KT, Olivares-Villagómez D. Granzyme B prevents aberrant IL-17 production and intestinal pathogenicity in CD4+ T cells. Mucosal Immunol [print-electronic]. 2021 Sep; 14(5): 1088-99. PMID: 34183776, PMCID: PMC8380717, PII: 10.1038/s41385-021-00427-1, DOI: 10.1038/s41385-021-00427-1, ISSN: 1935-3456.

Nazmi A, McClanahan KG, Olivares-Villagomez D. Unconventional Intestinal Intraepithelial Lymphocytes in Health and Disease. Crit Rev Immunol. 2021; 41(4): 23-38. PMID: 35381141, PII: 256bb987559bc247,79a368be1098d5b6, DOI: 10.1615/CritRevImmunol.2021039957, ISSN: 1040-8401.

Jayawardena D, Tyagi S, Nazmi A, Olivares-Villagómez D, Dudeja PK. Ion Transport Basis of Diarrhea in a Mouse Model of Adoptive T Cell Transfer Colitis. Dig Dis Sci [print-electronic]. 2020 Jun; 65(6): 1700-9. PMID: 31741140, PMCID: PMC7230007, PII: 10.1007/s10620-019-05945-4, DOI: 10.1007/s10620-019-05945-4, ISSN: 1573-2568.

Nazmi A, Greer MJ, Hoek KL, Piazuelo MB, Weitkamp JH, Olivares-Villagómez D. Osteopontin and iCD8a Cells Promote Intestinal Intraepithelial Lymphocyte Homeostasis. J Immunol [print-electronic]. 2020 Apr 4/1/2020; 204(7): 1968-81. PMID: 32102904, PMCID: PMC7175606, PII: jimmunol.1901168, DOI: 10.4049/jimmunol.1901168, ISSN: 1550-6606.

Yasmin S, Dixon BREA, Olivares-Villagómez D, Algood HMS. Interleukin-21 (IL-21) Downregulates Dendritic Cell Cytokine Responses to Helicobacter pylori and Modulates T Lymphocyte IL-17A Expression in Peyer's Patches during Infection. Infect Immun [electronic-print]. 2019 Nov; 87(11): PMID: 31383743, PMCID: PMC6803323, PII: IAI.00237-19, DOI: 10.1128/IAI.00237-19, ISSN: 1098-5522.

Nazmi A, Hoek KL, Greer MJ, Piazuelo MB, Minato N, Olivares-Villagómez D. Innate CD8aa+ cells promote ILC1-like intraepithelial lymphocyte homeostasis and intestinal inflammation. PLoS One. 2019; 14(7): e0215883. PMID: 31291255, PMCID: PMC6619599, PII: PONE-D-19-09774, DOI: 10.1371/journal.pone.0215883, ISSN: 1932-6203.

Van Kaer L, Olivares-Villagómez D. Development, Homeostasis, and Functions of Intestinal Intraepithelial Lymphocytes. J. Immunol. 2018 Apr 4/1/2018; 200(7): 2235-44. PMID: 29555677, PMCID: PMC5863587, PII: 200/7/2235, DOI: 10.4049/jimmunol.1701704, ISSN: 1550-6606.

Olivares-Villagómez D, Van Kaer L. Intestinal Intraepithelial Lymphocytes: Sentinels of the Mucosal Barrier. Trends Immunol [print-electronic]. 2017 Dec 12/5/2017; PMID: 29221933, PII: S1471-4906(17)30213-2, DOI: 10.1016/j.it.2017.11.003, ISSN: 1471-4981.

Kumar AA, Delgado AG, Piazuelo MB, Van Kaer L, Olivares-Villagómez D. Innate CD8aa(+) lymphocytes enhance anti-CD40 antibody-mediated colitis in mice. Immun Inflamm Dis [print-electronic]. 2017 Jun; 5(2): 109-23. PMID: 28474503, PMCID: PMC5418141, DOI: 10.1002/iid3.146, ISSN: 2050-4527.

Uzhachenko R, Boyd K, Olivares-Villagomez D, Zhu Y, Goodwin JS, Rana T, Shanker A, Tan WJ, Bondar T, Medzhitov R, Ivanova AV. Mitochondrial protein Fus1/Tusc2 in premature aging and age-related pathologies: critical roles of calcium and energy homeostasis. Aging (Albany NY). 2017 Mar 3/26/2017; 9(3): 627-49. PMID: 28351997, PMCID: PMC5391223, PII: 101213, DOI: 10.18632/aging.101213, ISSN: 1945-4589.

Pabbisetty SK, Rabacal W, Volanakis EJ, Parekh VV, Olivares-Villagómez D, Cendron D, Boyd KL, Van Kaer L, Sebzda E. Peripheral tolerance can be modified by altering KLF2-regulated Treg migration. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2016 Aug 8/9/2016; 113(32): E4662-70. PMID: 27462110, PMCID: PMC4987800, PII: 1605849113, DOI: 10.1073/pnas.1605849113, ISSN: 1091-6490.

Olivares-Villagómez D, Van Kaer L. ICD8a cells: living at the edge of the intestinal immune system [editorial]. Oncotarget. 2015 Aug 8/21/2015; 6(24): 19964-5. PMID: 26197090, PMCID: PMC4652978, PII: 4782, DOI: 10.18632/oncotarget.4782, ISSN: 1949-2553.

Ahmad R, Chaturvedi R, Olivares-Villagómez D, Habib T, Asim M, Shivesh P, Polk DB, Wilson KT, Washington MK, Van Kaer L, Dhawan P, Singh AB. Targeted colonic claudin-2 expression renders resistance to epithelial injury, induces immune suppression, and protects from colitis. Mucosal Immunol [print-electronic]. 2014 Nov; 7(6): 1340-53. PMID: 24670427, PMCID: PMC4221190, PII: mi201421, DOI: 10.1038/mi.2014.21, ISSN: 1935-3456.

Van Kaer L, Algood HM, Singh K, Parekh VV, Greer MJ, Piazuelo MB, Weitkamp JH, Matta P, Chaturvedi R, Wilson KT, Olivares-Villagómez D. CD8aa¿ innate-type lymphocytes in the intestinal epithelium mediate mucosal immunity. Immunity [print-electronic]. 2014 Sep 9/18/2014; 41(3): 451-64. PMID: 25220211, PMCID: PMC4169715, PII: S1074-7613(14)00304-5, DOI: 10.1016/j.immuni.2014.08.010, ISSN: 1097-4180.

Carbo A, Olivares-Villagómez D, Hontecillas R, Bassaganya-Riera J, Chaturvedi R, Piazuelo MB, Delgado A, Washington MK, Wilson KT, Algood HM. Systems modeling of the role of interleukin-21 in the maintenance of effector CD4+ T cell responses during chronic Helicobacter pylori infection. MBio. 2014 Jul 7/22/2014; 5(4): e01243-14. PMID: 25053783, PMCID: PMC4120195, PII: mBio.01243-14, DOI: 10.1128/mBio.01243-14, ISSN: 2150-7511.

Parekh VV, Wu L, Boyd KL, Williams JA, Gaddy JA, Olivares-Villagómez D, Cover TL, Zong WX, Zhang J, Van Kaer L. Impaired autophagy, defective T cell homeostasis, and a wasting syndrome in mice with a T cell-specific deletion of Vps34. J. Immunol [print-electronic]. 2013 May 5/15/2013; 190(10): 5086-101. PMID: 23596309, PMCID: PMC3646937, PII: jimmunol.1202071, DOI: 10.4049/jimmunol.1202071, ISSN: 1550-6606.

Parekh VV, Wu L, Olivares-Villagómez D, Wilson KT, Van Kaer L. Activated invariant NKT cells control central nervous system autoimmunity in a mechanism that involves myeloid-derived suppressor cells. J. Immunol [print-electronic]. 2013 Mar 3/1/2013; 190(5): 1948-60. PMID: 23345328, PMCID: PMC3577977, PII: jimmunol.1201718, DOI: 10.4049/jimmunol.1201718, ISSN: 1550-6606.

Van Kaer L, Rabacal WA, Scott Algood HM, Parekh VV, Olivares-Villagómez D. In vitro induction of regulatory CD4+CD8a+ T cells by TGF-ß, IL-7 and IFN-¿. PLoS ONE [electronic-print]. 2013; 8(7): e67821. PMID: 23844100, PMCID: PMC3701067, PII: PONE-D-13-11341, DOI: 10.1371/journal.pone.0067821, ISSN: 1932-6203.

Olivares-Villagómez D, Algood HM, Singh K, Parekh VV, Ryan KE, Piazuelo MB, Wilson KT, Van Kaer L. Intestinal epithelial cells modulate CD4 T cell responses via the thymus leukemia antigen. J. Immunol [print-electronic]. 2011 Oct 10/15/2011; 187(8): 4051-60. PMID: 21900182, PMCID: PMC3186831, PII: jimmunol.1101052, DOI: 10.4049/jimmunol.1101052, ISSN: 1550-6606.

Goettel JA, Scott Algood HM, Olivares-Villagómez D, Washington MK, Chaturvedi R, Wilson KT, Van Kaer L, Polk DB. KSR1 protects from interleukin-10 deficiency-induced colitis in mice by suppressing T-lymphocyte interferon-¿ production. Gastroenterology [print-electronic]. 2011 Jan; 140(1): 265-74. PMID: 20875416, PMCID: PMC3008308, PII: S0016-5085(10)01421-6, DOI: 10.1053/j.gastro.2010.09.041, ISSN: 1528-0012.

Olivares-Villagómez D, Van Kaer L. TL and CD8aa: Enigmatic partners in mucosal immunity. Immunol. Lett [print-electronic]. 2010 Nov 11/30/2010; 134(1): 1-6. PMID: 20850477, PMCID: PMC2967663, PII: S0165-2478(10)00224-5, DOI: 10.1016/j.imlet.2010.09.004, ISSN: 1879-0542.

Olivares-Villagómez D, Mendez-Fernandez YV, Parekh VV, Lalani S, Vincent TL, Cheroutre H, Van Kaer L. Thymus leukemia antigen controls intraepithelial lymphocyte function and inflammatory bowel disease. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2008 Nov 11/18/2008; 105(46): 17931-6. PMID: 19004778, PMCID: PMC2584730, PII: 0808242105, DOI: 10.1073/pnas.0808242105, ISSN: 1091-6490.

Yan J, Parekh VV, Mendez-Fernandez Y, Olivares-Villagómez D, Dragovic S, Hill T, Roopenian DC, Joyce S, Van Kaer L. In vivo role of ER-associated peptidase activity in tailoring peptides for presentation by MHC class Ia and class Ib molecules. J. Exp. Med [print-electronic]. 2006 Mar 3/20/2006; 203(3): 647-59. PMID: 16505142, PMCID: PMC2118255, PII: jem.20052271, DOI: 10.1084/jem.20052271, ISSN: 0022-1007.

Shen S, Ding Y, Tadokoro CE, Olivares-Villagómez D, Camps-Ramírez M, Curotto de Lafaille MA, Lafaille JJ. Control of homeostatic proliferation by regulatory T cells. J. Clin. Invest [print-electronic]. 2005 Dec; 115(12): 3517-26. PMID: 16294223, PMCID: PMC1283941, DOI: 10.1172/JCI25463, ISSN: 0021-9738.

Parekh VV, Wilson MT, Olivares-Villagómez D, Singh AK, Wu L, Wang CR, Joyce S, Van Kaer L. Glycolipid antigen induces long-term natural killer T cell anergy in mice. J. Clin. Invest. 2005 Sep; 115(9): 2572-83. PMID: 16138194, PMCID: PMC1193878, DOI: 10.1172/JCI24762, ISSN: 0021-9738.

Curotto de Lafaille MA, Shen S, Olivares-Villagómez D, Camps-Ramírez M, Lafaille JJ. Do regulatory T cells play a role in the control of homeostatic proliferation?. Int. Rev. Immunol. 2005 May; 24(3-4): 269-84. PMID: 16036378, PII: M107311R17237855, DOI: 10.1080/08830180590935001, ISSN: 0883-0185.

Parekh VV, Singh AK, Wilson MT, Olivares-Villagómez D, Bezbradica JS, Inazawa H, Ehara H, Sakai T, Serizawa I, Wu L, Wang CR, Joyce S, Van Kaer L. Quantitative and qualitative differences in the in vivo response of NKT cells to distinct alpha- and beta-anomeric glycolipids. J. Immunol. 2004 Sep 9/15/2004; 173(6): 3693-706. PMID: 15356115, PII: 173/6/3693, ISSN: 0022-1767.

Zhang J, Dong M, Li L, Fan Y, Pathre P, Dong J, Lou D, Wells JM, Olivares-Villagómez D, Van Kaer L, Wang X, Xu M. Endonuclease G is required for early embryogenesis and normal apoptosis in mice. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2003 Dec 12/23/2003; 100(26): 15782-7. PMID: 14663139, PMCID: PMC307645, PII: 2636393100, DOI: 10.1073/pnas.2636393100, ISSN: 0027-8424.

Wilson MT, Johansson C, Olivares-Villagómez D, Singh AK, Stanic AK, Wang CR, Joyce S, Wick MJ, Van Kaer L. The response of natural killer T cells to glycolipid antigens is characterized by surface receptor down-modulation and expansion. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2003 Sep 9/16/2003; 100(19): 10913-8. PMID: 12960397, PMCID: PMC196902, PII: 1833166100, DOI: 10.1073/pnas.1833166100, ISSN: 0027-8424.

Lane JE, Ribeiro-Rodrigues R, Olivares-Villagómez D, Vnencak-Jones CL, McCurley TL, Carter CE. Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction. Mem. Inst. Oswaldo Cruz [print-electronic]. 2003 Apr; 98(3): 373-6. PMID: 12886417, PII: S0074-02762003000300013, ISSN: 0074-0276.

Singh AK, Wilson MT, Hong S, Olivares-Villagómez D, Du C, Stanic AK, Joyce S, Sriram S, Koezuka Y, Van Kaer L. Natural killer T cell activation protects mice against experimental autoimmune encephalomyelitis. J. Exp. Med. 2001 Dec 12/17/2001; 194(12): 1801-11. PMID: 11748281, PMCID: PMC2193577, ISSN: 0022-1007.

Curotto de Lafaille MA, Muriglan S, Sunshine MJ, Lei Y, Kutchukhidze N, Furtado GC, Wensky AK, Olivares-Villagómez D, Lafaille JJ. Hyper immunoglobulin E response in mice with monoclonal populations of B and T lymphocytes. J. Exp. Med. 2001 Nov 11/5/2001; 194(9): 1349-59. PMID: 11696599, PMCID: PMC2195981, ISSN: 0022-1007.

Furtado GC, Olivares-Villagómez D, Curotto de Lafaille MA, Wensky AK, Latkowski JA, Lafaille JJ. Regulatory T cells in spontaneous autoimmune encephalomyelitis. Immunol. Rev. 2001 Aug; 182: 122-34. PMID: 11722629, PII: 1820110, ISSN: 0105-2896.

Olivares-Villagómez D, Wensky AK, Wang Y, Lafaille JJ. Repertoire requirements of CD4+ T cells that prevent spontaneous autoimmune encephalomyelitis. J. Immunol. 2000 May 5/15/2000; 164(10): 5499-507. PMID: 10799918, PII: ji_v164n10p5499, ISSN: 0022-1767.

Olivares-Villagómez D, Wang Y, Lafaille JJ. Regulatory CD4(+) T cells expressing endogenous T cell receptor chains protect myelin basic protein-specific transgenic mice from spontaneous autoimmune encephalomyelitis. J. Exp. Med. 1998 Nov 11/16/1998; 188(10): 1883-94. PMID: 9815266, PMCID: PMC2212402, ISSN: 0022-1007.

Olivares-Villagómez D, McCurley TL, Vnencak-Jones CL, Correa-Oliveira R, Colley DG, Carter CE. Polymerase chain reaction amplification of three different Trypanosoma cruzi DNA sequences from human chagasic cardiac tissue. Am. J. Trop. Med. Hyg. 1998 Oct; 59(4): 563-70. PMID: 9790431, ISSN: 0002-9637.

Lane JE, Olivares-Villagomez D, Vnencak-Jones CL, McCurley TL, Carter CE. Detection of Trypanosoma cruzi with the polymerase chain reaction and in situ hybridization in infected murine cardiac tissue. Am. J. Trop. Med. Hyg. 1997 Jun; 56(6): 588-95. PMID: 9230784, ISSN: 0002-9637.

Olivares-Villagómez, D., Algood, H.M., Parekh, V.V., Singh, K., Ryan, K., M. Blanca Piazuelo, Wilson, K.T., and Van Kaer, L. Intestinal epithelial cells modulate CD4 T cell responses via the thymus leukemia antigen. Journal of Immunology.

Huang, Y., Park, Y., Wang-Zhu, Y., Larange, A., Arens, R., Bernardol, I., Olivares-Villagómez, D., Herndler-Brandstetter, D., Abraham, N., Grubeck-Loebenstein, B., Schoenberger, S.P., Van Kaer, L., Kronenberg, M., Teitell, A., and Cheroutre, H. Mucosal CD8 memory T cells are selected in the periphery by an MHC class I molecule. Nature Immunology.

Available Postdoctoral Position Details
Posted: 12/17/2020

The laboratory of Dr. Olivares-Villagómez is looking for a candidate for a 3-year postdoctoral position. This individual should or plan to have a Ph.D. degree or equivalent, preferentially with expertise on the area of molecular and/or cellular immunology. The candidate should be able to work independently, be capable of fully grasping the relevant literature on the field, formulate appropriate hypothesis and experiments, and be creative. Experience with mouse models is preferred, although not a requirement. The candidate should possess excellent English conversational and writing skills. All the members in the laboratory of Dr. Olivares-Villagómez work as a team, and we expect the candidate to have a positive attitude and be a good lab citizen. Dr. Olivares-Villagómez investigates different aspects of mucosal immunology. One of the main projects relates to the biology of intestinal intraepithelial lymphocytes (IEL), with special emphasis on inflammatory diseases. His laboratory also studies the role of two molecules in intestinal homeostasis: osteopontin and granzyme B. The laboratory of Dr. Olivares-Villagómez is part of the Department of Pathology, Microbiology and Immunology at Vanderbilt University Medical Center and the Vanderbilt Institute for Infection, Immunology and Inflammation. VUMC is at the vanguard of biomedical research with a vibrant scientific community and within the top 20 universities with substantial funding from the National Institutes of Health. VUMC is well equipped for biomedical research and possess state-of-the-art cores for sequencing, flow cytometry, mass spectrometry, etc. VUMC is located in Nashville, Tennessee, an energetic city recognized for its eclectic music, friendly Southern atmosphere, high standard of living, great food and extensive green areas. Individuals interested in this position should send their CV, brief statement of research experience/interests, and contact information for at least two references. Please submit applications to: danyvid.olivares-villagomez@vumc.org