Faculty Appointments
Professor of Cell and Developmental Biology
Professor of Biochemistry
Education
Ph.D., Biology , University of Sydney, Sydney, AustraliaB.Sc., Biology, University of Sydney, Sydney, Australia
Office Address
Vanderbilt University Medical Center
Suite 4140 MRB III
Nashville, TN 37232-8240
Suite 4140 MRB III
Nashville, TN 37232-8240
Research Description
Research Description: We study transcriptional regulation in normal and cancer cells. Research in the laboratory is focused squarely on understanding how the oncoprotein transcription factor MYC works and how it can be targeted for therapeutic effect in cancer cells.
The term “MYC” refers to a family of three related oncogenes that are broadly over-expressed in cancer and contribute to an estimated 100,000 cancer deaths annually in the United States alone. MYC proteins function as transcriptional regulators, controlling the expression of thousands of genes linked to cell growth, proliferation, metabolism, and genomic instability. MYC proteins are highly-validated but challenging drug targets for cancer therapy. Our work seeks to expose basic mechanisms of MYC action that can lead to new strategies to target MYC in the clinic. We study interactions of MYC with chromatin regulators in multiple cancer contexts, and collaborate to develop small molecule inhibitors of one particular chromatin-associated MYC co-factor, WDR5, and to explore their utility as anti-cancer agents.
The term “MYC” refers to a family of three related oncogenes that are broadly over-expressed in cancer and contribute to an estimated 100,000 cancer deaths annually in the United States alone. MYC proteins function as transcriptional regulators, controlling the expression of thousands of genes linked to cell growth, proliferation, metabolism, and genomic instability. MYC proteins are highly-validated but challenging drug targets for cancer therapy. Our work seeks to expose basic mechanisms of MYC action that can lead to new strategies to target MYC in the clinic. We study interactions of MYC with chromatin regulators in multiple cancer contexts, and collaborate to develop small molecule inhibitors of one particular chromatin-associated MYC co-factor, WDR5, and to explore their utility as anti-cancer agents.
Research Keywords
Cancer, chromatin, transcription, gene expression, cancer therapy
Publications
Moe KC, Maxwell JN, Wang J, Jones CA, Csaki GT, Florian AC, Romer AS, Bryant DL, Farone AL, Liu Q, Tansey WP, Weissmiller AM. The SWI/SNF ATPase BRG1 facilitates multiple pro-tumorigenic gene expression programs in SMARCB1-deficient cancer cells. Oncogenesis. 2022 Jun 6/1/2022; 11(1): 30. PMID: 35650187, PMCID: PMC9160003, PII: 10.1038/s41389-022-00406-6, DOI: 10.1038/s41389-022-00406-6, ISSN: 2157-9024.
Metz EP, Wilder PJ, Popay TM, Wang J, Liu Q, Kalluchi A, Rowley MJ, Tansey WP, Rizzino A. Elevating SOX2 Downregulates MYC through a SOX2:MYC Signaling Axis and Induces a Slowly Cycling Proliferative State in Human Tumor Cells. Cancers (Basel). 2022 Apr 4/12/2022; 14(8): PMID: 35454854, PMCID: PMC9025961, PII: cancers14081946, DOI: 10.3390/cancers14081946, ISSN: 2072-6694.
Florian AC, Woodley CM, Wang J, Grieb BC, Slota MJ, Guerrazzi K, Hsu CY, Matlock BK, Flaherty DK, Lorey SL, Fesik SW, Howard GC, Liu Q, Weissmiller AM, Tansey WP. Synergistic action of WDR5 and HDM2 inhibitors in SMARCB1-deficient cancer cells. NAR Cancer. 2022 Mar; 4(1): zcac007. PMID: 35252869, PMCID: PMC8892060, PII: zcac007, DOI: 10.1093/narcan/zcac007, ISSN: 2632-8674.
Siladi AJ, Wang J, Florian AC, Thomas LR, Creighton JH, Matlock BK, Flaherty DK, Lorey SL, Howard GC, Fesik SW, Weissmiller AM, Liu Q, Tansey WP. WIN site inhibition disrupts a subset of WDR5 function. Sci Rep. 2022 Feb 2/3/2022; 12(1): 1848. PMID: 35115608, PMCID: PMC8813994, PII: 10.1038/s41598-022-05947-9, DOI: 10.1038/s41598-022-05947-9, ISSN: 2045-2322.
Jones CA, Tansey WP, Weissmiller AM. Emerging Themes in Mechanisms of Tumorigenesis by SWI/SNF Subunit Mutation. Epigenet Insights. 2022; 15: 25168657221115656. PMID: 35911061, PMCID: PMC9329810, PII: 10.1177_25168657221115656, DOI: 10.1177/25168657221115656, ISSN: 2516-8657.
Guarnaccia AD, Weissmiller AM, Tansey WP. Gene-specific quantification of nascent transcription following targeted degradation of endogenous proteins in cultured cells. STAR Protoc. 2021 Dec 12/17/2021; 2(4): 101000. PMID: 34917979, PMCID: PMC8669106, PII: S2666-1667(21)00706-1, DOI: 10.1016/j.xpro.2021.101000, ISSN: 2666-1667.
Woodley CM, Romer AS, Wang J, Guarnaccia AD, Elion DL, Maxwell JN, Guerrazzi K, McCann TS, Popay TM, Matlock BK, Flaherty DK, Lorey SL, Liu Q, Tansey WP, Weissmiller AM. Multiple interactions of the oncoprotein transcription factor MYC with the SWI/SNF chromatin remodeler. Oncogene [print-electronic]. 2021 May; 40(20): 3593-609. PMID: 33931740, PMCID: PMC8141032, PII: 10.1038/s41388-021-01804-7, DOI: 10.1038/s41388-021-01804-7, ISSN: 1476-5594.
Wolpaw AJ, Bayliss R, Büchel G, Dang CV, Eilers M, Gustafson WC, Hansen GM, Jura N, Knapp S, Lemmon MA, Levens D, Maris JM, Marmorstein R, Metallo SJ, Park JR, Penn LZ, Rape M, Roussel MF, Shokat KM, Tansey WP, Verba KA, Vos SM, Weiss WA, Wolf E, Mossé YP. Drugging the 'undruggable' MYCN oncogenic transcription factor: Overcoming previous obstacles to impact childhood cancers. Cancer Res [print-electronic]. 2021 Jan 1/28/2021; PMID: 33509943, PII: 0008-5472.CAN-20-3108, DOI: 10.1158/0008-5472.CAN-20-3108, ISSN: 1538-7445.
Guarnaccia AD, Rose KL, Wang J, Zhao B, Popay TM, Wang CE, Guerrazzi K, Hill S, Woodley CM, Hansen TJ, Lorey SL, Shaw JG, Payne WG, Weissmiller AM, Olejniczak ET, Fesik SW, Liu Q, Tansey WP. Impact of WIN site inhibitor on the WDR5 interactome. Cell Rep. 2021 Jan 1/19/2021; 34(3): 108636. PMID: 33472061, PMCID: PMC7871196, PII: S2211-1247(20)31625-9, DOI: 10.1016/j.celrep.2020.108636, ISSN: 2211-1247.
Popay TM, Wang J, Adams CM, Howard GC, Codreanu SG, Sherrod SD, McLean JA, Thomas LR, Lorey SL, Machida YJ, Weissmiller AM, Eischen CM, Liu Q, Tansey WP. MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with host cell factor-1. Elife. 2021 Jan 1/8/2021; 10: PMID: 33416496, PMCID: PMC7793627, PII: 60191, DOI: 10.7554/eLife.60191, ISSN: 2050-084X.
Chacón Simon S, Wang F, Thomas LR, Phan J, Zhao B, Olejniczak ET, Macdonald JD, Shaw JG, Schlund C, Payne W, Creighton J, Stauffer SR, Waterson AG, Tansey WP, Fesik SW. Discovery of WD Repeat-Containing Protein 5 (WDR5)-MYC Inhibitors Using Fragment-Based Methods and Structure-Based Design. J Med Chem [print-electronic]. 2020 Apr 4/23/2020; 63(8): 4315-33. PMID: 32223236, PMCID: PMC7187413, DOI: 10.1021/acs.jmedchem.0c00224, ISSN: 1520-4804.
Bryan AF, Wang J, Howard GC, Guarnaccia AD, Woodley CM, Aho ER, Rellinger EJ, Matlock BK, Flaherty DK, Lorey SL, Chung DH, Fesik SW, Liu Q, Weissmiller AM, Tansey WP. WDR5 is a conserved regulator of protein synthesis gene expression. Nucleic Acids Res. 2020 Apr 4/6/2020; 48(6): 2924-41. PMID: 31996893, PMCID: PMC7102967, PII: 5717749, DOI: 10.1093/nar/gkaa051, ISSN: 1362-4962.
Thomas LR, Adams CM, Fesik SW, Eischen CM, Tansey WP. Targeting MYC through WDR5. Mol Cell Oncol. 2020; 7(2): 1709388. PMID: 32158922, PMCID: PMC7051159, PII: 1709388, DOI: 10.1080/23723556.2019.1709388, ISSN: 2372-3556.
Macdonald JD, Chacón Simon S, Han C, Wang F, Shaw JG, Howes JE, Sai J, Yuh JP, Camper D, Alicie BM, Alvarado J, Nikhar S, Payne W, Aho ER, Bauer JA, Zhao B, Phan J, Thomas LR, Rossanese OW, Tansey WP, Waterson AG, Stauffer SR, Fesik SW. Discovery and Optimization of Salicylic Acid-Derived Sulfonamide Inhibitors of the WD Repeat-Containing Protein 5-MYC Protein-Protein Interaction. J Med Chem [print-electronic]. 2019 Dec 12/26/2019; 62(24): 11232-59. PMID: 31724864, PMCID: PMC6933084, DOI: 10.1021/acs.jmedchem.9b01411, ISSN: 1520-4804.
Thomas LR, Adams CM, Wang J, Weissmiller AM, Creighton J, Lorey SL, Liu Q, Fesik SW, Eischen CM, Tansey WP. Interaction of the oncoprotein transcription factor MYC with its chromatin cofactor WDR5 is essential for tumor maintenance. Proc Natl Acad Sci U S A [print-electronic]. 2019 Dec 12/10/2019; 116(50): 25260-8. PMID: 31767764, PMCID: PMC6911241, PII: 1910391116, DOI: 10.1073/pnas.1910391116, ISSN: 1091-6490.
Weissmiller AM, Wang J, Lorey SL, Howard GC, Martinez E, Liu Q, Tansey WP. Inhibition of MYC by the SMARCB1 tumor suppressor. Nat Commun. 2019 May 5/1/2019; 10(1): 2014. PMID: 31043611, PMCID: PMC6494882, PII: 10.1038/s41467-019-10022-5, DOI: 10.1038/s41467-019-10022-5, ISSN: 2041-1723.
Aho ER, Wang J, Gogliotti RD, Howard GC, Phan J, Acharya P, Macdonald JD, Cheng K, Lorey SL, Lu B, Wenzel S, Foshage AM, Alvarado J, Wang F, Shaw JG, Zhao B, Weissmiller AM, Thomas LR, Vakoc CR, Hall MD, Hiebert SW, Liu Q, Stauffer SR, Fesik SW, Tansey WP. Displacement of WDR5 from Chromatin by a WIN Site Inhibitor with Picomolar Affinity. Cell Rep. 2019 Mar 3/12/2019; 26(11): 2916-2928.e13. PMID: 30865883, PMCID: PMC6448596, PII: S2211-1247(19)30218-9, DOI: 10.1016/j.celrep.2019.02.047, ISSN: 2211-1247.
Aho ER, Wang J, Gogliotti RD, Howard GC, Phan J, Acharya P, Macdonald JD, Cheng K, Lorey SL, Lu B, Wenzel S, Foshage AM, Alvarado J, Wang F, Shaw JG, Zhao B, Weissmiller AM, Thomas LR, Vakoc CR, Hall MD, Hiebert SW, Liu Q, Stauffer SR, Fesik SW, Tansey WP. Displacement of WDR5 from Chromatin by a WIN Site Inhibitor with Picomolar Affinity. Cell Rep. 2019 Mar 3/12/2019; 26(11): 2916-2928.e13. PMID: 30865883, PMCID: PMC6448596, PII: S2211-1247(19)30218-9, DOI: 10.1016/j.celrep.2019.02.047, ISSN: 2211-1247.
Aho ER, Weissmiller AM, Fesik SW, Tansey WP. Targeting WDR5: A WINning Anti-Cancer Strategy?. Epigenet Insights. 2019; 12: 2516865719865282. PMID: 31360909, PMCID: PMC6640055, PII: 10.1177_2516865719865282, DOI: 10.1177/2516865719865282, ISSN: 2516-8657.
Ng VH, Hang BI, Sawyer LM, Neitzel LR, Crispi EE, Rose KL, Popay TM, Zhong A, Lee LA, Tansey WP, Huppert S, Lee E. Phosphorylation of XIAP at threonine 180 controls its activity in Wnt signaling. J. Cell. Sci [print-electronic]. 2018 Apr 4/20/2018; PMID: 29678905, PII: jcs.210575, DOI: 10.1242/jcs.210575, ISSN: 1477-9137.
Guarnaccia AD, Tansey WP. Moonlighting with WDR5: A Cellular Multitasker. J Clin Med. 2018 Jan 1/30/2018; 7(2): PMID: 29385767, PMCID: PMC5852437, PII: jcm7020021, DOI: 10.3390/jcm7020021, ISSN: 2077-0383.
Parang B, Kaz AM, Barrett CW, Short SP, Ning W, Keating CE, Mittal MK, Naik RD, Washington MK, Revetta FL, Smith JJ, Chen X, Wilson KT, Brand T, Bader DM, Tansey WP, Chen R, Brentnall TA, Grady WM, Williams CS. BVES regulates c-Myc stability via PP2A and suppresses colitis-induced tumourigenesis. Gut [print-electronic]. 2017 May; 66(5): 852-62. PMID: 28389570, PMCID: PMC5385850, PII: gutjnl-2015-310255, DOI: 10.1136/gutjnl-2015-310255, ISSN: 1468-3288.
Howard GC, Tansey WP. Interaction of Gcn4 with target gene chromatin is modulated by proteasome function. Mol. Biol. Cell [print-electronic]. 2016 Sep 9/1/2016; 27(17): 2735-41. PMID: 27385344, PMCID: PMC5007093, PII: mbc.E16-03-0192, DOI: 10.1091/mbc.E16-03-0192, ISSN: 1939-4586.
Thomas LR, Foshage AM, Weissmiller AM, Popay TM, Grieb BC, Qualls SJ, Ng V, Carboneau B, Lorey S, Eischen CM, Tansey WP. Interaction of MYC with host cell factor-1 is mediated by the evolutionarily conserved Myc box IV motif. Oncogene [print-electronic]. 2016 Jul 7/7/2016; 35(27): 3613-8. PMID: 26522729, PMCID: PMC4853269, PII: onc2015416, DOI: 10.1038/onc.2015.416, ISSN: 1476-5594.
McCann TS, Guo Y, McDonald WH, Tansey WP. Antagonistic roles for the ubiquitin ligase Asr1 and the ubiquitin-specific protease Ubp3 in subtelomeric gene silencing. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2016 Feb 2/2/2016; 113(5): 1309-14. PMID: 26787877, PMCID: PMC4747757, PII: 1518375113, DOI: 10.1073/pnas.1518375113, ISSN: 1091-6490.
Thomas LR, Foshage AM, Weissmiller AM, Tansey WP. The MYC-WDR5 Nexus and Cancer. Cancer Res [print-electronic]. 2015 Oct 10/1/2015; 75(19): 4012-5. PMID: 26383167, PMCID: PMC4592407, PII: 0008-5472.CAN-15-1216, DOI: 10.1158/0008-5472.CAN-15-1216, ISSN: 1538-7445.
Thomas LR, Wang Q, Grieb BC, Phan J, Foshage AM, Sun Q, Olejniczak ET, Clark T, Dey S, Lorey S, Alicie B, Howard GC, Cawthon B, Ess KC, Eischen CM, Zhao Z, Fesik SW, Tansey WP. Interaction with WDR5 promotes target gene recognition and tumorigenesis by MYC. Mol. Cell [print-electronic]. 2015 May 5/7/2015; 58(3): 440-52. PMID: 25818646, PMCID: PMC4427524, PII: S1097-2765(15)00142-2, DOI: 10.1016/j.molcel.2015.02.028, ISSN: 1097-4164.
Chakraborty AA, Scuoppo C, Dey S, Thomas LR, Lorey SL, Lowe SW, Tansey WP. A common functional consequence of tumor-derived mutations within c-MYC. Oncogene [print-electronic]. 2015 Apr 4/30/2015; 34(18): 2406-9. PMID: 24998853, PMCID: PMC4286529, PII: onc2014186, DOI: 10.1038/onc.2014.186, ISSN: 1476-5594.
Chakraborty AA, Tansey WP. Do changes in the c-MYC coding sequence contribute to tumorigenesis?. Mol Cell Oncol. 2015 Apr; 2(2): e965631. PMID: 27308414, PMCID: PMC4904990, PII: 965631, DOI: 10.4161/23723548.2014.965631, ISSN: 2372-3556.
McCann TS, Tansey WP. Functions of the proteasome on chromatin. Biomolecules. 2014 Nov 11/21/2014; 4(4): 1026-44. PMID: 25422899, PMCID: PMC4279168, PII: biom4041026, DOI: 10.3390/biom4041026, ISSN: 2218-273X.
Galligan JJ, Rose KL, Beavers WN, Hill S, Tallman KA, Tansey WP, Marnett LJ. Stable histone adduction by 4-oxo-2-nonenal: a potential link between oxidative stress and epigenetics. J. Am. Chem. Soc [print-electronic]. 2014 Aug 8/27/2014; 136(34): 11864-6. PMID: 25099620, PMCID: PMC4151707, DOI: 10.1021/ja503604t, ISSN: 1520-5126.
Bonizec M, Hérissant L, Pokrzywa W, Geng F, Wenzel S, Howard GC, Rodriguez P, Krause S, Tansey WP, Hoppe T, Dargemont C. The ubiquitin-selective chaperone Cdc48/p97 associates with Ubx3 to modulate monoubiquitylation of histone H2B. Nucleic Acids Res [print-electronic]. 2014; 42(17): 10975-86. PMID: 25183520, PMCID: PMC4176170, PII: gku786, DOI: 10.1093/nar/gku786, ISSN: 1362-4962.
Geng F, Tansey WP. Similar temporal and spatial recruitment of native 19S and 20S proteasome subunits to transcriptionally active chromatin. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Apr 4/17/2012; 109(16): 6060-5. PMID: 22474342, PMCID: PMC3341028, PII: 1200854109, DOI: 10.1073/pnas.1200854109, ISSN: 1091-6490.
Howard GC, Collins GA, Tansey WP. Letter to the editor. Chemical-genetic strategy for inhibiting proteasome function in Saccharomyces cerevisiae. Yeast [print-electronic]. 2012 Feb; 29(2): 93-4. PMID: 22162073, PMCID: PMC3657583, DOI: 10.1002/yea.1919, ISSN: 1097-0061.
Geng F, Wenzel S, Tansey WP. Ubiquitin and proteasomes in transcription. Annu. Rev. Biochem [print-electronic]. 2012; 81: 177-201. PMID: 22404630, PMCID: PMC3637986, DOI: 10.1146/annurev-biochem-052110-120012, ISSN: 1545-4509.
Leung A, Cajigas I, Jia P, Ezhkova E, Brickner JH, Zhao Z, Geng F, Tansey WP. Histone H2B ubiquitylation and H3 lysine 4 methylation prevent ectopic silencing of euchromatic loci important for the cellular response to heat. Mol. Biol. Cell [print-electronic]. 2011 Aug 8/1/2011; 22(15): 2741-53. PMID: 21680712, PMCID: PMC3145549, PII: mbc.E11-05-0426, DOI: 10.1091/mbc.E11-05-0426, ISSN: 1939-4586.
Simpson DR, Yu M, Zheng S, Zhao Z, Muthuswamy SK, Tansey WP. Epithelial cell organization suppresses Myc function by attenuating Myc expression. Cancer Res [print-electronic]. 2011 Jun 6/1/2011; 71(11): 3822-30. PMID: 21610111, PMCID: PMC3347713, PII: 0008-5472.CAN-10-3782, DOI: 10.1158/0008-5472.CAN-10-3782, ISSN: 1538-7445.
Lee SB, Kim JJ, Chung JS, Lee MS, Lee KH, Kim BS, Do Yoo Y. Romo1 is a negative-feedback regulator of Myc. J. Cell. Sci [print-electronic]. 2011 Jun 6/1/2011; 124(Pt 11): 1911-24. PMID: 21558421, PII: jcs.079996, DOI: 10.1242/jcs.079996, ISSN: 1477-9137.
Thomas LR, Tansey WP. Proteolytic control of the oncoprotein transcription factor Myc. Adv. Cancer Res. 2011; 110: 77-106. PMID: 21704229, PII: B978-0-12-386469-7.00004-9, DOI: 10.1016/B978-0-12-386469-7.00004-9, ISSN: 0065-230X.
Zheng S, Tansey WP, Hiebert SW, Zhao Z. Integrative network analysis identifies key genes and pathways in the progression of hepatitis C virus induced hepatocellular carcinoma. BMC Med Genomics. 2011; 4: 62. PMID: 21824427, PMCID: PMC3212927, PII: 1755-8794-4-62, DOI: 10.1186/1755-8794-4-62, ISSN: 1755-8794.
Collins GA, Gomez TA, Deshaies RJ, Tansey WP. Combined chemical and genetic approach to inhibit proteolysis by the proteasome. Yeast. 2010 Nov; 27(11): 965-74. PMID: 20625982, PMCID: PMC3566228, DOI: 10.1002/yea.1805, ISSN: 1097-0061.
Wang X, Muratani M, Tansey WP, Ptashne M. Proteolytic instability and the action of nonclassical transcriptional activators. Curr. Biol [print-electronic]. 2010 May 5/11/2010; 20(9): 868-71. PMID: 20417106, PMCID: PMC3033761, PII: S0960-9822(10)00354-4, DOI: 10.1016/j.cub.2010.03.029, ISSN: 1879-0445.
Collins GA, Lipford JR, Deshaies RJ, Tansey WP. Gal4 turnover and transcription activation. Nature. 2009 Oct 10/8/2009; 461(7265): E7; discussion E8. PMID: 19812621, PMCID: PMC3072683, PII: nature08406, DOI: 10.1038/nature08406, ISSN: 1476-4687.
Chakraborty AA, Tansey WP. Inference of cell cycle-dependent proteolysis by laser scanning cytometry. Exp. Cell Res [print-electronic]. 2009 Jun 6/10/2009; 315(10): 1772-8. PMID: 19331831, PMCID: PMC2680938, PII: S0014-4827(09)00035-4, DOI: 10.1016/j.yexcr.2009.01.011, ISSN: 1090-2422.
Daulny A, Tansey WP. Damage control: DNA repair, transcription, and the ubiquitin-proteasome system. DNA Repair (Amst.) [print-electronic]. 2009 Apr 4/5/2009; 8(4): 444-8. PMID: 19272841, PII: S1568-7864(09)00009-3, DOI: 10.1016/j.dnarep.2009.01.017, ISSN: 1568-7864.
Chakraborty AA, Tansey WP. Adenoviral E1A function through Myc. Cancer Res. 2009 Jan 1/1/2009; 69(1): 6-9. PMID: 19117980, PII: 69/1/6, DOI: 10.1158/0008-5472.CAN-08-3026, ISSN: 1538-7445.
Daulny A, Geng F, Muratani M, Geisinger JM, Salghetti SE, Tansey WP. Modulation of RNA polymerase II subunit composition by ubiquitylation. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2008 Dec 12/16/2008; 105(50): 19649-54. PMID: 19064926, PMCID: PMC2604917, PII: 0809372105, DOI: 10.1073/pnas.0809372105, ISSN: 1091-6490.
Geng F, Tansey WP. Polyubiquitylation of histone H2B. Mol. Biol. Cell [print-electronic]. 2008 Sep; 19(9): 3616-24. PMID: 18562693, PMCID: PMC2526708, PII: E08-01-0050, DOI: 10.1091/mbc.E08-01-0050, ISSN: 1939-4586.
Kurland JF, Tansey WP. Myc-mediated transcriptional repression by recruitment of histone deacetylase. Cancer Res. 2008 May 5/15/2008; 68(10): 3624-9. PMID: 18483244, PII: 68/10/3624, DOI: 10.1158/0008-5472.CAN-07-6552, ISSN: 1538-7445.
Tworkowski KA, Chakraborty AA, Samuelson AV, Seger YR, Narita M, Hannon GJ, Lowe SW, Tansey WP. Adenovirus E1A targets p400 to induce the cellular oncoprotein Myc. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2008 Apr 4/22/2008; 105(16): 6103-8. PMID: 18413597, PMCID: PMC2329713, PII: 0802095105, DOI: 10.1073/pnas.0802095105, ISSN: 1091-6490.
Leung A, Geng F, Daulny A, Collins G, Guzzardo P, Tansey WP. Transcriptional control and the ubiquitin-proteasome system. Ernst Schering Found Symp Proc. 2008; (1): 75-97. PMID: 19198065.
Pal B, Chan NC, Helfenbaum L, Tan K, Tansey WP, Gething MJ. SCFCdc4-mediated degradation of the Hac1p transcription factor regulates the unfolded protein response in Saccharomyces cerevisiae. Mol. Biol. Cell [print-electronic]. 2007 Feb; 18(2): 426-40. PMID: 17108329, PMCID: PMC1783797, PII: E06-04-0304, DOI: 10.1091/mbc.E06-04-0304, ISSN: 1059-1524.
Tansey WP. Denaturing protein immunoprecipitation from yeast. CSH Protoc. 2007; 2007: pdb.prot4643. PMID: 21356925.
Tansey WP. Yeast Chromatin Immunoprecipitation (ChIP) Assay. CSH Protoc. 2007; 2007: pdb.prot4642. PMID: 21356924.
Tansey WP. Pulse-chase assay for measuring protein stability in yeast. CSH Protoc. 2007; 2007: pdb.prot4641. PMID: 21356923.
Tansey WP. Nondenaturing protein immunoprecipitation from Mammalian cells. CSH Protoc. 2007; 2007: pdb.prot4620. PMID: 21356922.
Collins GA, Tansey WP. The proteasome: a utility tool for transcription?. Curr. Opin. Genet. Dev [print-electronic]. 2006 Apr; 16(2): 197-202. PMID: 16503126, PII: S0959-437X(06)00031-1, DOI: 10.1016/j.gde.2006.02.009, ISSN: 0959-437X.
Ezhkova E, Tansey WP. Chromatin immunoprecipitation to study protein-DNA interactions in budding yeast. Methods Mol. Biol. 2006; 313: 225-44. PMID: 16118437, PII: 1-59259-958-3:225, DOI: 10.1385/1-59259-958-3:225, ISSN: 1064-3745.
Lee D, Ezhkova E, Li B, Pattenden SG, Tansey WP, Workman JL. The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators. Cell. 2005 Nov 11/4/2005; 123(3): 423-36. PMID: 16269334, PII: S0092-8674(05)00819-6, DOI: 10.1016/j.cell.2005.08.015, ISSN: 0092-8674.
Hemann MT, Bric A, Teruya-Feldstein J, Herbst A, Nilsson JA, Cordon-Cardo C, Cleveland JL, Tansey WP, Lowe SW. Evasion of the p53 tumour surveillance network by tumour-derived MYC mutants. Nature. 2005 Aug 8/11/2005; 436(7052): 807-11. PMID: 16094360, PMCID: PMC4599579, PII: nature03845, DOI: 10.1038/nature03845, ISSN: 1476-4687.
Muratani M, Kung C, Shokat KM, Tansey WP. The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing. Cell. 2005 Mar 3/25/2005; 120(6): 887-99. PMID: 15797387, PII: S0092-8674(04)01250-4, DOI: 10.1016/j.cell.2004.12.025, ISSN: 0092-8674.
Herbst A, Hemann MT, Tworkowski KA, Salghetti SE, Lowe SW, Tansey WP. A conserved element in Myc that negatively regulates its proapoptotic activity. EMBO Rep. 2005 Feb; 6(2): 177-83. PMID: 15678160, PMCID: PMC1299243, PII: 7400333, DOI: 10.1038/sj.embor.7400333, ISSN: 1469-221X.
Tansey WP. Death, destruction, and the proteasome. N. Engl. J. Med. 2004 Jul 7/22/2004; 351(4): 393-4. PMID: 15269322, PII: 351/4/393, DOI: 10.1056/NEJMcibr041505, ISSN: 1533-4406.
Herbst A, Salghetti SE, Kim SY, Tansey WP. Multiple cell-type-specific elements regulate Myc protein stability. Oncogene. 2004 May 5/6/2004; 23(21): 3863-71. PMID: 15021906, PII: 1207492, DOI: 10.1038/sj.onc.1207492, ISSN: 0950-9232.
Kurland JF, Tansey WP. Crashing waves of destruction: the cell cycle and APC(Cdh1) regulation of SCF(Skp2) [editorial]. Cancer Cell. 2004 Apr; 5(4): 305-6. PMID: 15093536, PII: S1535610804000911, ISSN: 1535-6108.
Tubon TC, Tansey WP, Herr W. A nonconserved surface of the TFIIB zinc ribbon domain plays a direct role in RNA polymerase II recruitment. Mol. Cell. Biol. 2004 Apr; 24(7): 2863-74. PMID: 15024075, PMCID: PMC371104, ISSN: 0270-7306.
Janicki SM, Tsukamoto T, Salghetti SE, Tansey WP, Sachidanandam R, Prasanth KV, Ried T, Shav-Tal Y, Bertrand E, Singer RH, Spector DL. From silencing to gene expression: real-time analysis in single cells. Cell. 2004 Mar 3/5/2004; 116(5): 683-98. PMID: 15006351, PII: S0092867404001710, ISSN: 0092-8674.
Ezhkova E, Tansey WP. Proteasomal ATPases link ubiquitylation of histone H2B to methylation of histone H3. Mol. Cell. 2004 Feb 2/13/2004; 13(3): 435-42. PMID: 14967150, PII: S1097276504000267, ISSN: 1097-2765.
Kim SY, Herbst A, Tworkowski KA, Salghetti SE, Tansey WP. Skp2 regulates Myc protein stability and activity. Mol. Cell. 2003 May; 11(5): 1177-88. PMID: 12769843, PII: S1097276503001734, ISSN: 1097-2765.
Muratani M, Tansey WP. How the ubiquitin-proteasome system controls transcription. Nat. Rev. Mol. Cell Biol. 2003 Mar; 4(3): 192-201. PMID: 12612638, PII: nrm1049, DOI: 10.1038/nrm1049, ISSN: 1471-0072.
Tworkowski KA, Salghetti SE, Tansey WP. Stable and unstable pools of Myc protein exist in human cells. Oncogene. 2002 Dec 12/5/2002; 21(55): 8515-20. PMID: 12466972, DOI: 10.1038/sj.onc.1205976, ISSN: 0950-9232.
Alarcon-Vargas D, Tansey WP, Ronai Z. Regulation of c-myc stability by selective stress conditions and by MEKK1 requires aa 127-189 of c-myc. Oncogene. 2002 Jun 6/27/2002; 21(28): 4384-91. PMID: 12080469, DOI: 10.1038/sj.onc.1205543, ISSN: 0950-9232.
Méndez J, Zou-Yang XH, Kim SY, Hidaka M, Tansey WP, Stillman B. Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication. Mol. Cell. 2002 Mar; 9(3): 481-91. PMID: 11931757, PII: S1097276502004677, ISSN: 1097-2765.
Ejkova E, Tansey WP. Old dogs and new tricks: meeting on mechanisms of eukaryotic transcription. EMBO Rep. 2002 Mar; 3(3): 219-23. PMID: 11882540, PMCID: PMC1084018, PII: 3/3/219, DOI: 10.1093/embo-reports/kvf054, ISSN: 1469-221X.
Salghetti SE, Caudy AA, Chenoweth JG, Tansey WP. Regulation of transcriptional activation domain function by ubiquitin. Science [print-electronic]. 2001 Aug 8/31/2001; 293(5535): 1651-3. PMID: 11463878, PII: 1062079, DOI: 10.1126/science.1062079, ISSN: 0036-8075.
Tansey WP. Transcriptional activation: risky business. Genes Dev. 2001 May 5/1/2001; 15(9): 1045-50. PMID: 11331599, DOI: 10.1101/gad.896501, ISSN: 0890-9369.
Tansey WP. Transcriptional regulation: RUPture in the ER. Nature cell biology. 2000 Oct; 2(10) Sect. E175-7.
Salghetti SE, Muratani M, Wijnen H, Futcher B, Tansey WP. Functional overlap of sequences that activate transcription and signal ubiquitin-mediated proteolysis. Proc. Natl. Acad. Sci. U.S.A. 2000 Mar 3/28/2000; 97(7): 3118-23. PMID: 10706616, PMCID: PMC16202, PII: 050007597, DOI: 10.1073/pnas.050007597, ISSN: 0027-8424.
Herbst A, Tansey WP. HAM: a new epitope-tag for in vivo protein labeling. Mol. Biol. Rep. 2000; 27(4): 203-8. PMID: 11455955, ISSN: 0301-4851.
Tansey WP. How cells use proteolysis to control their growth. Mol. Med. 1999 Dec; 5(12): 773-82. PMID: 10666476, PMCID: PMC2230494, PII: 0209, ISSN: 1076-1551.
Salghetti SE, Kim SY, Tansey WP. Destruction of Myc by ubiquitin-mediated proteolysis: cancer-associated and transforming mutations stabilize Myc. EMBO J. 1999 Feb 2/1/1999; 18(3): 717-26. PMID: 9927431, PMCID: PMC1171164, DOI: 10.1093/emboj/18.3.717, ISSN: 0261-4189.
Tansey WP, Herr W. TAFs: guilt by association?. Cell. 1997 Mar 3/21/1997; 88(6): 729-32. PMID: 9118213, PII: S0092-8674(00)81916-9, ISSN: 0092-8674.
Tansey WP, Herr W. Selective use of TBP and TFIIB revealed by a TATA-TBP-TFIIB array with altered specificity. Science. 1997 Feb 2/7/1997; 275(5301): 829-31. PMID: 9012349, ISSN: 0036-8075.
Pendergrast PS, Morrison D, Tansey WP, Hernandez N. Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly. J. Virol. 1996 Aug; 70(8): 5025-34. PMID: 8764009, PMCID: PMC190456, ISSN: 0022-538X.
Tansey WP, Herr W. The ability to associate with activation domains in vitro is not required for the TATA box-binding protein to support activated transcription in vivo. Proc. Natl. Acad. Sci. U.S.A. 1995 Nov 11/7/1995; 92(23): 10550-4. PMID: 7479838, PMCID: PMC40649, ISSN: 0027-8424.
Tansey WP, Ruppert S, Tjian R, Herr W. Multiple regions of TBP participate in the response to transcriptional activators in vivo. Genes Dev. 1994 Nov 11/15/1994; 8(22): 2756-69. PMID: 7958931, ISSN: 0890-9369.
Gilbert MT, Sun J, Yan Y, Oddoux C, Lazarus A, Tansey WP, Lavin TN, Catanzaro DF. Renin gene promoter activity in GC cells is regulated by cAMP and thyroid hormone through Pit-1-dependent mechanisms. J. Biol. Chem. 1994 Nov 11/11/1994; 269(45): 28049-54. PMID: 7961740, ISSN: 0021-9258.
Tansey WP, Schaufele F, Heslewood M, Handford C, Reudelhuber TL, Catanzaro DF. Distance-dependent interactions between basal, cyclic AMP, and thyroid hormone response elements in the rat growth hormone promoter. J. Biol. Chem. 1993 Jul 7/15/1993; 268(20): 14906-11. PMID: 8392063, ISSN: 0021-9258.
Henderson BR, Tansey WP, Phillips SM, Ramshaw IA, Kefford RF. Transcriptional and posttranscriptional activation of urokinase plasminogen activator gene expression in metastatic tumor cells. Cancer Res. 1992 May 5/1/1992; 52(9): 2489-96. PMID: 1568219, ISSN: 0008-5472.
Tansey WP, Catanzaro DF. Sp1 and thyroid hormone receptor differentially activate expression of human growth hormone and chorionic somatomammotropin genes. J. Biol. Chem. 1991 May 5/25/1991; 266(15): 9805-13. PMID: 2033067, ISSN: 0021-9258.
Metz EP, Wilder PJ, Popay TM, Wang J, Liu Q, Kalluchi A, Rowley MJ, Tansey WP, Rizzino A. Elevating SOX2 Downregulates MYC through a SOX2:MYC Signaling Axis and Induces a Slowly Cycling Proliferative State in Human Tumor Cells. Cancers (Basel). 2022 Apr 4/12/2022; 14(8): PMID: 35454854, PMCID: PMC9025961, PII: cancers14081946, DOI: 10.3390/cancers14081946, ISSN: 2072-6694.
Florian AC, Woodley CM, Wang J, Grieb BC, Slota MJ, Guerrazzi K, Hsu CY, Matlock BK, Flaherty DK, Lorey SL, Fesik SW, Howard GC, Liu Q, Weissmiller AM, Tansey WP. Synergistic action of WDR5 and HDM2 inhibitors in SMARCB1-deficient cancer cells. NAR Cancer. 2022 Mar; 4(1): zcac007. PMID: 35252869, PMCID: PMC8892060, PII: zcac007, DOI: 10.1093/narcan/zcac007, ISSN: 2632-8674.
Siladi AJ, Wang J, Florian AC, Thomas LR, Creighton JH, Matlock BK, Flaherty DK, Lorey SL, Howard GC, Fesik SW, Weissmiller AM, Liu Q, Tansey WP. WIN site inhibition disrupts a subset of WDR5 function. Sci Rep. 2022 Feb 2/3/2022; 12(1): 1848. PMID: 35115608, PMCID: PMC8813994, PII: 10.1038/s41598-022-05947-9, DOI: 10.1038/s41598-022-05947-9, ISSN: 2045-2322.
Jones CA, Tansey WP, Weissmiller AM. Emerging Themes in Mechanisms of Tumorigenesis by SWI/SNF Subunit Mutation. Epigenet Insights. 2022; 15: 25168657221115656. PMID: 35911061, PMCID: PMC9329810, PII: 10.1177_25168657221115656, DOI: 10.1177/25168657221115656, ISSN: 2516-8657.
Guarnaccia AD, Weissmiller AM, Tansey WP. Gene-specific quantification of nascent transcription following targeted degradation of endogenous proteins in cultured cells. STAR Protoc. 2021 Dec 12/17/2021; 2(4): 101000. PMID: 34917979, PMCID: PMC8669106, PII: S2666-1667(21)00706-1, DOI: 10.1016/j.xpro.2021.101000, ISSN: 2666-1667.
Woodley CM, Romer AS, Wang J, Guarnaccia AD, Elion DL, Maxwell JN, Guerrazzi K, McCann TS, Popay TM, Matlock BK, Flaherty DK, Lorey SL, Liu Q, Tansey WP, Weissmiller AM. Multiple interactions of the oncoprotein transcription factor MYC with the SWI/SNF chromatin remodeler. Oncogene [print-electronic]. 2021 May; 40(20): 3593-609. PMID: 33931740, PMCID: PMC8141032, PII: 10.1038/s41388-021-01804-7, DOI: 10.1038/s41388-021-01804-7, ISSN: 1476-5594.
Wolpaw AJ, Bayliss R, Büchel G, Dang CV, Eilers M, Gustafson WC, Hansen GM, Jura N, Knapp S, Lemmon MA, Levens D, Maris JM, Marmorstein R, Metallo SJ, Park JR, Penn LZ, Rape M, Roussel MF, Shokat KM, Tansey WP, Verba KA, Vos SM, Weiss WA, Wolf E, Mossé YP. Drugging the 'undruggable' MYCN oncogenic transcription factor: Overcoming previous obstacles to impact childhood cancers. Cancer Res [print-electronic]. 2021 Jan 1/28/2021; PMID: 33509943, PII: 0008-5472.CAN-20-3108, DOI: 10.1158/0008-5472.CAN-20-3108, ISSN: 1538-7445.
Guarnaccia AD, Rose KL, Wang J, Zhao B, Popay TM, Wang CE, Guerrazzi K, Hill S, Woodley CM, Hansen TJ, Lorey SL, Shaw JG, Payne WG, Weissmiller AM, Olejniczak ET, Fesik SW, Liu Q, Tansey WP. Impact of WIN site inhibitor on the WDR5 interactome. Cell Rep. 2021 Jan 1/19/2021; 34(3): 108636. PMID: 33472061, PMCID: PMC7871196, PII: S2211-1247(20)31625-9, DOI: 10.1016/j.celrep.2020.108636, ISSN: 2211-1247.
Popay TM, Wang J, Adams CM, Howard GC, Codreanu SG, Sherrod SD, McLean JA, Thomas LR, Lorey SL, Machida YJ, Weissmiller AM, Eischen CM, Liu Q, Tansey WP. MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with host cell factor-1. Elife. 2021 Jan 1/8/2021; 10: PMID: 33416496, PMCID: PMC7793627, PII: 60191, DOI: 10.7554/eLife.60191, ISSN: 2050-084X.
Chacón Simon S, Wang F, Thomas LR, Phan J, Zhao B, Olejniczak ET, Macdonald JD, Shaw JG, Schlund C, Payne W, Creighton J, Stauffer SR, Waterson AG, Tansey WP, Fesik SW. Discovery of WD Repeat-Containing Protein 5 (WDR5)-MYC Inhibitors Using Fragment-Based Methods and Structure-Based Design. J Med Chem [print-electronic]. 2020 Apr 4/23/2020; 63(8): 4315-33. PMID: 32223236, PMCID: PMC7187413, DOI: 10.1021/acs.jmedchem.0c00224, ISSN: 1520-4804.
Bryan AF, Wang J, Howard GC, Guarnaccia AD, Woodley CM, Aho ER, Rellinger EJ, Matlock BK, Flaherty DK, Lorey SL, Chung DH, Fesik SW, Liu Q, Weissmiller AM, Tansey WP. WDR5 is a conserved regulator of protein synthesis gene expression. Nucleic Acids Res. 2020 Apr 4/6/2020; 48(6): 2924-41. PMID: 31996893, PMCID: PMC7102967, PII: 5717749, DOI: 10.1093/nar/gkaa051, ISSN: 1362-4962.
Thomas LR, Adams CM, Fesik SW, Eischen CM, Tansey WP. Targeting MYC through WDR5. Mol Cell Oncol. 2020; 7(2): 1709388. PMID: 32158922, PMCID: PMC7051159, PII: 1709388, DOI: 10.1080/23723556.2019.1709388, ISSN: 2372-3556.
Macdonald JD, Chacón Simon S, Han C, Wang F, Shaw JG, Howes JE, Sai J, Yuh JP, Camper D, Alicie BM, Alvarado J, Nikhar S, Payne W, Aho ER, Bauer JA, Zhao B, Phan J, Thomas LR, Rossanese OW, Tansey WP, Waterson AG, Stauffer SR, Fesik SW. Discovery and Optimization of Salicylic Acid-Derived Sulfonamide Inhibitors of the WD Repeat-Containing Protein 5-MYC Protein-Protein Interaction. J Med Chem [print-electronic]. 2019 Dec 12/26/2019; 62(24): 11232-59. PMID: 31724864, PMCID: PMC6933084, DOI: 10.1021/acs.jmedchem.9b01411, ISSN: 1520-4804.
Thomas LR, Adams CM, Wang J, Weissmiller AM, Creighton J, Lorey SL, Liu Q, Fesik SW, Eischen CM, Tansey WP. Interaction of the oncoprotein transcription factor MYC with its chromatin cofactor WDR5 is essential for tumor maintenance. Proc Natl Acad Sci U S A [print-electronic]. 2019 Dec 12/10/2019; 116(50): 25260-8. PMID: 31767764, PMCID: PMC6911241, PII: 1910391116, DOI: 10.1073/pnas.1910391116, ISSN: 1091-6490.
Weissmiller AM, Wang J, Lorey SL, Howard GC, Martinez E, Liu Q, Tansey WP. Inhibition of MYC by the SMARCB1 tumor suppressor. Nat Commun. 2019 May 5/1/2019; 10(1): 2014. PMID: 31043611, PMCID: PMC6494882, PII: 10.1038/s41467-019-10022-5, DOI: 10.1038/s41467-019-10022-5, ISSN: 2041-1723.
Aho ER, Wang J, Gogliotti RD, Howard GC, Phan J, Acharya P, Macdonald JD, Cheng K, Lorey SL, Lu B, Wenzel S, Foshage AM, Alvarado J, Wang F, Shaw JG, Zhao B, Weissmiller AM, Thomas LR, Vakoc CR, Hall MD, Hiebert SW, Liu Q, Stauffer SR, Fesik SW, Tansey WP. Displacement of WDR5 from Chromatin by a WIN Site Inhibitor with Picomolar Affinity. Cell Rep. 2019 Mar 3/12/2019; 26(11): 2916-2928.e13. PMID: 30865883, PMCID: PMC6448596, PII: S2211-1247(19)30218-9, DOI: 10.1016/j.celrep.2019.02.047, ISSN: 2211-1247.
Aho ER, Wang J, Gogliotti RD, Howard GC, Phan J, Acharya P, Macdonald JD, Cheng K, Lorey SL, Lu B, Wenzel S, Foshage AM, Alvarado J, Wang F, Shaw JG, Zhao B, Weissmiller AM, Thomas LR, Vakoc CR, Hall MD, Hiebert SW, Liu Q, Stauffer SR, Fesik SW, Tansey WP. Displacement of WDR5 from Chromatin by a WIN Site Inhibitor with Picomolar Affinity. Cell Rep. 2019 Mar 3/12/2019; 26(11): 2916-2928.e13. PMID: 30865883, PMCID: PMC6448596, PII: S2211-1247(19)30218-9, DOI: 10.1016/j.celrep.2019.02.047, ISSN: 2211-1247.
Aho ER, Weissmiller AM, Fesik SW, Tansey WP. Targeting WDR5: A WINning Anti-Cancer Strategy?. Epigenet Insights. 2019; 12: 2516865719865282. PMID: 31360909, PMCID: PMC6640055, PII: 10.1177_2516865719865282, DOI: 10.1177/2516865719865282, ISSN: 2516-8657.
Ng VH, Hang BI, Sawyer LM, Neitzel LR, Crispi EE, Rose KL, Popay TM, Zhong A, Lee LA, Tansey WP, Huppert S, Lee E. Phosphorylation of XIAP at threonine 180 controls its activity in Wnt signaling. J. Cell. Sci [print-electronic]. 2018 Apr 4/20/2018; PMID: 29678905, PII: jcs.210575, DOI: 10.1242/jcs.210575, ISSN: 1477-9137.
Guarnaccia AD, Tansey WP. Moonlighting with WDR5: A Cellular Multitasker. J Clin Med. 2018 Jan 1/30/2018; 7(2): PMID: 29385767, PMCID: PMC5852437, PII: jcm7020021, DOI: 10.3390/jcm7020021, ISSN: 2077-0383.
Parang B, Kaz AM, Barrett CW, Short SP, Ning W, Keating CE, Mittal MK, Naik RD, Washington MK, Revetta FL, Smith JJ, Chen X, Wilson KT, Brand T, Bader DM, Tansey WP, Chen R, Brentnall TA, Grady WM, Williams CS. BVES regulates c-Myc stability via PP2A and suppresses colitis-induced tumourigenesis. Gut [print-electronic]. 2017 May; 66(5): 852-62. PMID: 28389570, PMCID: PMC5385850, PII: gutjnl-2015-310255, DOI: 10.1136/gutjnl-2015-310255, ISSN: 1468-3288.
Howard GC, Tansey WP. Interaction of Gcn4 with target gene chromatin is modulated by proteasome function. Mol. Biol. Cell [print-electronic]. 2016 Sep 9/1/2016; 27(17): 2735-41. PMID: 27385344, PMCID: PMC5007093, PII: mbc.E16-03-0192, DOI: 10.1091/mbc.E16-03-0192, ISSN: 1939-4586.
Thomas LR, Foshage AM, Weissmiller AM, Popay TM, Grieb BC, Qualls SJ, Ng V, Carboneau B, Lorey S, Eischen CM, Tansey WP. Interaction of MYC with host cell factor-1 is mediated by the evolutionarily conserved Myc box IV motif. Oncogene [print-electronic]. 2016 Jul 7/7/2016; 35(27): 3613-8. PMID: 26522729, PMCID: PMC4853269, PII: onc2015416, DOI: 10.1038/onc.2015.416, ISSN: 1476-5594.
McCann TS, Guo Y, McDonald WH, Tansey WP. Antagonistic roles for the ubiquitin ligase Asr1 and the ubiquitin-specific protease Ubp3 in subtelomeric gene silencing. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2016 Feb 2/2/2016; 113(5): 1309-14. PMID: 26787877, PMCID: PMC4747757, PII: 1518375113, DOI: 10.1073/pnas.1518375113, ISSN: 1091-6490.
Thomas LR, Foshage AM, Weissmiller AM, Tansey WP. The MYC-WDR5 Nexus and Cancer. Cancer Res [print-electronic]. 2015 Oct 10/1/2015; 75(19): 4012-5. PMID: 26383167, PMCID: PMC4592407, PII: 0008-5472.CAN-15-1216, DOI: 10.1158/0008-5472.CAN-15-1216, ISSN: 1538-7445.
Thomas LR, Wang Q, Grieb BC, Phan J, Foshage AM, Sun Q, Olejniczak ET, Clark T, Dey S, Lorey S, Alicie B, Howard GC, Cawthon B, Ess KC, Eischen CM, Zhao Z, Fesik SW, Tansey WP. Interaction with WDR5 promotes target gene recognition and tumorigenesis by MYC. Mol. Cell [print-electronic]. 2015 May 5/7/2015; 58(3): 440-52. PMID: 25818646, PMCID: PMC4427524, PII: S1097-2765(15)00142-2, DOI: 10.1016/j.molcel.2015.02.028, ISSN: 1097-4164.
Chakraborty AA, Scuoppo C, Dey S, Thomas LR, Lorey SL, Lowe SW, Tansey WP. A common functional consequence of tumor-derived mutations within c-MYC. Oncogene [print-electronic]. 2015 Apr 4/30/2015; 34(18): 2406-9. PMID: 24998853, PMCID: PMC4286529, PII: onc2014186, DOI: 10.1038/onc.2014.186, ISSN: 1476-5594.
Chakraborty AA, Tansey WP. Do changes in the c-MYC coding sequence contribute to tumorigenesis?. Mol Cell Oncol. 2015 Apr; 2(2): e965631. PMID: 27308414, PMCID: PMC4904990, PII: 965631, DOI: 10.4161/23723548.2014.965631, ISSN: 2372-3556.
McCann TS, Tansey WP. Functions of the proteasome on chromatin. Biomolecules. 2014 Nov 11/21/2014; 4(4): 1026-44. PMID: 25422899, PMCID: PMC4279168, PII: biom4041026, DOI: 10.3390/biom4041026, ISSN: 2218-273X.
Galligan JJ, Rose KL, Beavers WN, Hill S, Tallman KA, Tansey WP, Marnett LJ. Stable histone adduction by 4-oxo-2-nonenal: a potential link between oxidative stress and epigenetics. J. Am. Chem. Soc [print-electronic]. 2014 Aug 8/27/2014; 136(34): 11864-6. PMID: 25099620, PMCID: PMC4151707, DOI: 10.1021/ja503604t, ISSN: 1520-5126.
Bonizec M, Hérissant L, Pokrzywa W, Geng F, Wenzel S, Howard GC, Rodriguez P, Krause S, Tansey WP, Hoppe T, Dargemont C. The ubiquitin-selective chaperone Cdc48/p97 associates with Ubx3 to modulate monoubiquitylation of histone H2B. Nucleic Acids Res [print-electronic]. 2014; 42(17): 10975-86. PMID: 25183520, PMCID: PMC4176170, PII: gku786, DOI: 10.1093/nar/gku786, ISSN: 1362-4962.
Geng F, Tansey WP. Similar temporal and spatial recruitment of native 19S and 20S proteasome subunits to transcriptionally active chromatin. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Apr 4/17/2012; 109(16): 6060-5. PMID: 22474342, PMCID: PMC3341028, PII: 1200854109, DOI: 10.1073/pnas.1200854109, ISSN: 1091-6490.
Howard GC, Collins GA, Tansey WP. Letter to the editor. Chemical-genetic strategy for inhibiting proteasome function in Saccharomyces cerevisiae. Yeast [print-electronic]. 2012 Feb; 29(2): 93-4. PMID: 22162073, PMCID: PMC3657583, DOI: 10.1002/yea.1919, ISSN: 1097-0061.
Geng F, Wenzel S, Tansey WP. Ubiquitin and proteasomes in transcription. Annu. Rev. Biochem [print-electronic]. 2012; 81: 177-201. PMID: 22404630, PMCID: PMC3637986, DOI: 10.1146/annurev-biochem-052110-120012, ISSN: 1545-4509.
Leung A, Cajigas I, Jia P, Ezhkova E, Brickner JH, Zhao Z, Geng F, Tansey WP. Histone H2B ubiquitylation and H3 lysine 4 methylation prevent ectopic silencing of euchromatic loci important for the cellular response to heat. Mol. Biol. Cell [print-electronic]. 2011 Aug 8/1/2011; 22(15): 2741-53. PMID: 21680712, PMCID: PMC3145549, PII: mbc.E11-05-0426, DOI: 10.1091/mbc.E11-05-0426, ISSN: 1939-4586.
Simpson DR, Yu M, Zheng S, Zhao Z, Muthuswamy SK, Tansey WP. Epithelial cell organization suppresses Myc function by attenuating Myc expression. Cancer Res [print-electronic]. 2011 Jun 6/1/2011; 71(11): 3822-30. PMID: 21610111, PMCID: PMC3347713, PII: 0008-5472.CAN-10-3782, DOI: 10.1158/0008-5472.CAN-10-3782, ISSN: 1538-7445.
Lee SB, Kim JJ, Chung JS, Lee MS, Lee KH, Kim BS, Do Yoo Y. Romo1 is a negative-feedback regulator of Myc. J. Cell. Sci [print-electronic]. 2011 Jun 6/1/2011; 124(Pt 11): 1911-24. PMID: 21558421, PII: jcs.079996, DOI: 10.1242/jcs.079996, ISSN: 1477-9137.
Thomas LR, Tansey WP. Proteolytic control of the oncoprotein transcription factor Myc. Adv. Cancer Res. 2011; 110: 77-106. PMID: 21704229, PII: B978-0-12-386469-7.00004-9, DOI: 10.1016/B978-0-12-386469-7.00004-9, ISSN: 0065-230X.
Zheng S, Tansey WP, Hiebert SW, Zhao Z. Integrative network analysis identifies key genes and pathways in the progression of hepatitis C virus induced hepatocellular carcinoma. BMC Med Genomics. 2011; 4: 62. PMID: 21824427, PMCID: PMC3212927, PII: 1755-8794-4-62, DOI: 10.1186/1755-8794-4-62, ISSN: 1755-8794.
Collins GA, Gomez TA, Deshaies RJ, Tansey WP. Combined chemical and genetic approach to inhibit proteolysis by the proteasome. Yeast. 2010 Nov; 27(11): 965-74. PMID: 20625982, PMCID: PMC3566228, DOI: 10.1002/yea.1805, ISSN: 1097-0061.
Wang X, Muratani M, Tansey WP, Ptashne M. Proteolytic instability and the action of nonclassical transcriptional activators. Curr. Biol [print-electronic]. 2010 May 5/11/2010; 20(9): 868-71. PMID: 20417106, PMCID: PMC3033761, PII: S0960-9822(10)00354-4, DOI: 10.1016/j.cub.2010.03.029, ISSN: 1879-0445.
Collins GA, Lipford JR, Deshaies RJ, Tansey WP. Gal4 turnover and transcription activation. Nature. 2009 Oct 10/8/2009; 461(7265): E7; discussion E8. PMID: 19812621, PMCID: PMC3072683, PII: nature08406, DOI: 10.1038/nature08406, ISSN: 1476-4687.
Chakraborty AA, Tansey WP. Inference of cell cycle-dependent proteolysis by laser scanning cytometry. Exp. Cell Res [print-electronic]. 2009 Jun 6/10/2009; 315(10): 1772-8. PMID: 19331831, PMCID: PMC2680938, PII: S0014-4827(09)00035-4, DOI: 10.1016/j.yexcr.2009.01.011, ISSN: 1090-2422.
Daulny A, Tansey WP. Damage control: DNA repair, transcription, and the ubiquitin-proteasome system. DNA Repair (Amst.) [print-electronic]. 2009 Apr 4/5/2009; 8(4): 444-8. PMID: 19272841, PII: S1568-7864(09)00009-3, DOI: 10.1016/j.dnarep.2009.01.017, ISSN: 1568-7864.
Chakraborty AA, Tansey WP. Adenoviral E1A function through Myc. Cancer Res. 2009 Jan 1/1/2009; 69(1): 6-9. PMID: 19117980, PII: 69/1/6, DOI: 10.1158/0008-5472.CAN-08-3026, ISSN: 1538-7445.
Daulny A, Geng F, Muratani M, Geisinger JM, Salghetti SE, Tansey WP. Modulation of RNA polymerase II subunit composition by ubiquitylation. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2008 Dec 12/16/2008; 105(50): 19649-54. PMID: 19064926, PMCID: PMC2604917, PII: 0809372105, DOI: 10.1073/pnas.0809372105, ISSN: 1091-6490.
Geng F, Tansey WP. Polyubiquitylation of histone H2B. Mol. Biol. Cell [print-electronic]. 2008 Sep; 19(9): 3616-24. PMID: 18562693, PMCID: PMC2526708, PII: E08-01-0050, DOI: 10.1091/mbc.E08-01-0050, ISSN: 1939-4586.
Kurland JF, Tansey WP. Myc-mediated transcriptional repression by recruitment of histone deacetylase. Cancer Res. 2008 May 5/15/2008; 68(10): 3624-9. PMID: 18483244, PII: 68/10/3624, DOI: 10.1158/0008-5472.CAN-07-6552, ISSN: 1538-7445.
Tworkowski KA, Chakraborty AA, Samuelson AV, Seger YR, Narita M, Hannon GJ, Lowe SW, Tansey WP. Adenovirus E1A targets p400 to induce the cellular oncoprotein Myc. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2008 Apr 4/22/2008; 105(16): 6103-8. PMID: 18413597, PMCID: PMC2329713, PII: 0802095105, DOI: 10.1073/pnas.0802095105, ISSN: 1091-6490.
Leung A, Geng F, Daulny A, Collins G, Guzzardo P, Tansey WP. Transcriptional control and the ubiquitin-proteasome system. Ernst Schering Found Symp Proc. 2008; (1): 75-97. PMID: 19198065.
Pal B, Chan NC, Helfenbaum L, Tan K, Tansey WP, Gething MJ. SCFCdc4-mediated degradation of the Hac1p transcription factor regulates the unfolded protein response in Saccharomyces cerevisiae. Mol. Biol. Cell [print-electronic]. 2007 Feb; 18(2): 426-40. PMID: 17108329, PMCID: PMC1783797, PII: E06-04-0304, DOI: 10.1091/mbc.E06-04-0304, ISSN: 1059-1524.
Tansey WP. Denaturing protein immunoprecipitation from yeast. CSH Protoc. 2007; 2007: pdb.prot4643. PMID: 21356925.
Tansey WP. Yeast Chromatin Immunoprecipitation (ChIP) Assay. CSH Protoc. 2007; 2007: pdb.prot4642. PMID: 21356924.
Tansey WP. Pulse-chase assay for measuring protein stability in yeast. CSH Protoc. 2007; 2007: pdb.prot4641. PMID: 21356923.
Tansey WP. Nondenaturing protein immunoprecipitation from Mammalian cells. CSH Protoc. 2007; 2007: pdb.prot4620. PMID: 21356922.
Collins GA, Tansey WP. The proteasome: a utility tool for transcription?. Curr. Opin. Genet. Dev [print-electronic]. 2006 Apr; 16(2): 197-202. PMID: 16503126, PII: S0959-437X(06)00031-1, DOI: 10.1016/j.gde.2006.02.009, ISSN: 0959-437X.
Ezhkova E, Tansey WP. Chromatin immunoprecipitation to study protein-DNA interactions in budding yeast. Methods Mol. Biol. 2006; 313: 225-44. PMID: 16118437, PII: 1-59259-958-3:225, DOI: 10.1385/1-59259-958-3:225, ISSN: 1064-3745.
Lee D, Ezhkova E, Li B, Pattenden SG, Tansey WP, Workman JL. The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators. Cell. 2005 Nov 11/4/2005; 123(3): 423-36. PMID: 16269334, PII: S0092-8674(05)00819-6, DOI: 10.1016/j.cell.2005.08.015, ISSN: 0092-8674.
Hemann MT, Bric A, Teruya-Feldstein J, Herbst A, Nilsson JA, Cordon-Cardo C, Cleveland JL, Tansey WP, Lowe SW. Evasion of the p53 tumour surveillance network by tumour-derived MYC mutants. Nature. 2005 Aug 8/11/2005; 436(7052): 807-11. PMID: 16094360, PMCID: PMC4599579, PII: nature03845, DOI: 10.1038/nature03845, ISSN: 1476-4687.
Muratani M, Kung C, Shokat KM, Tansey WP. The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing. Cell. 2005 Mar 3/25/2005; 120(6): 887-99. PMID: 15797387, PII: S0092-8674(04)01250-4, DOI: 10.1016/j.cell.2004.12.025, ISSN: 0092-8674.
Herbst A, Hemann MT, Tworkowski KA, Salghetti SE, Lowe SW, Tansey WP. A conserved element in Myc that negatively regulates its proapoptotic activity. EMBO Rep. 2005 Feb; 6(2): 177-83. PMID: 15678160, PMCID: PMC1299243, PII: 7400333, DOI: 10.1038/sj.embor.7400333, ISSN: 1469-221X.
Tansey WP. Death, destruction, and the proteasome. N. Engl. J. Med. 2004 Jul 7/22/2004; 351(4): 393-4. PMID: 15269322, PII: 351/4/393, DOI: 10.1056/NEJMcibr041505, ISSN: 1533-4406.
Herbst A, Salghetti SE, Kim SY, Tansey WP. Multiple cell-type-specific elements regulate Myc protein stability. Oncogene. 2004 May 5/6/2004; 23(21): 3863-71. PMID: 15021906, PII: 1207492, DOI: 10.1038/sj.onc.1207492, ISSN: 0950-9232.
Kurland JF, Tansey WP. Crashing waves of destruction: the cell cycle and APC(Cdh1) regulation of SCF(Skp2) [editorial]. Cancer Cell. 2004 Apr; 5(4): 305-6. PMID: 15093536, PII: S1535610804000911, ISSN: 1535-6108.
Tubon TC, Tansey WP, Herr W. A nonconserved surface of the TFIIB zinc ribbon domain plays a direct role in RNA polymerase II recruitment. Mol. Cell. Biol. 2004 Apr; 24(7): 2863-74. PMID: 15024075, PMCID: PMC371104, ISSN: 0270-7306.
Janicki SM, Tsukamoto T, Salghetti SE, Tansey WP, Sachidanandam R, Prasanth KV, Ried T, Shav-Tal Y, Bertrand E, Singer RH, Spector DL. From silencing to gene expression: real-time analysis in single cells. Cell. 2004 Mar 3/5/2004; 116(5): 683-98. PMID: 15006351, PII: S0092867404001710, ISSN: 0092-8674.
Ezhkova E, Tansey WP. Proteasomal ATPases link ubiquitylation of histone H2B to methylation of histone H3. Mol. Cell. 2004 Feb 2/13/2004; 13(3): 435-42. PMID: 14967150, PII: S1097276504000267, ISSN: 1097-2765.
Kim SY, Herbst A, Tworkowski KA, Salghetti SE, Tansey WP. Skp2 regulates Myc protein stability and activity. Mol. Cell. 2003 May; 11(5): 1177-88. PMID: 12769843, PII: S1097276503001734, ISSN: 1097-2765.
Muratani M, Tansey WP. How the ubiquitin-proteasome system controls transcription. Nat. Rev. Mol. Cell Biol. 2003 Mar; 4(3): 192-201. PMID: 12612638, PII: nrm1049, DOI: 10.1038/nrm1049, ISSN: 1471-0072.
Tworkowski KA, Salghetti SE, Tansey WP. Stable and unstable pools of Myc protein exist in human cells. Oncogene. 2002 Dec 12/5/2002; 21(55): 8515-20. PMID: 12466972, DOI: 10.1038/sj.onc.1205976, ISSN: 0950-9232.
Alarcon-Vargas D, Tansey WP, Ronai Z. Regulation of c-myc stability by selective stress conditions and by MEKK1 requires aa 127-189 of c-myc. Oncogene. 2002 Jun 6/27/2002; 21(28): 4384-91. PMID: 12080469, DOI: 10.1038/sj.onc.1205543, ISSN: 0950-9232.
Méndez J, Zou-Yang XH, Kim SY, Hidaka M, Tansey WP, Stillman B. Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication. Mol. Cell. 2002 Mar; 9(3): 481-91. PMID: 11931757, PII: S1097276502004677, ISSN: 1097-2765.
Ejkova E, Tansey WP. Old dogs and new tricks: meeting on mechanisms of eukaryotic transcription. EMBO Rep. 2002 Mar; 3(3): 219-23. PMID: 11882540, PMCID: PMC1084018, PII: 3/3/219, DOI: 10.1093/embo-reports/kvf054, ISSN: 1469-221X.
Salghetti SE, Caudy AA, Chenoweth JG, Tansey WP. Regulation of transcriptional activation domain function by ubiquitin. Science [print-electronic]. 2001 Aug 8/31/2001; 293(5535): 1651-3. PMID: 11463878, PII: 1062079, DOI: 10.1126/science.1062079, ISSN: 0036-8075.
Tansey WP. Transcriptional activation: risky business. Genes Dev. 2001 May 5/1/2001; 15(9): 1045-50. PMID: 11331599, DOI: 10.1101/gad.896501, ISSN: 0890-9369.
Tansey WP. Transcriptional regulation: RUPture in the ER. Nature cell biology. 2000 Oct; 2(10) Sect. E175-7.
Salghetti SE, Muratani M, Wijnen H, Futcher B, Tansey WP. Functional overlap of sequences that activate transcription and signal ubiquitin-mediated proteolysis. Proc. Natl. Acad. Sci. U.S.A. 2000 Mar 3/28/2000; 97(7): 3118-23. PMID: 10706616, PMCID: PMC16202, PII: 050007597, DOI: 10.1073/pnas.050007597, ISSN: 0027-8424.
Herbst A, Tansey WP. HAM: a new epitope-tag for in vivo protein labeling. Mol. Biol. Rep. 2000; 27(4): 203-8. PMID: 11455955, ISSN: 0301-4851.
Tansey WP. How cells use proteolysis to control their growth. Mol. Med. 1999 Dec; 5(12): 773-82. PMID: 10666476, PMCID: PMC2230494, PII: 0209, ISSN: 1076-1551.
Salghetti SE, Kim SY, Tansey WP. Destruction of Myc by ubiquitin-mediated proteolysis: cancer-associated and transforming mutations stabilize Myc. EMBO J. 1999 Feb 2/1/1999; 18(3): 717-26. PMID: 9927431, PMCID: PMC1171164, DOI: 10.1093/emboj/18.3.717, ISSN: 0261-4189.
Tansey WP, Herr W. TAFs: guilt by association?. Cell. 1997 Mar 3/21/1997; 88(6): 729-32. PMID: 9118213, PII: S0092-8674(00)81916-9, ISSN: 0092-8674.
Tansey WP, Herr W. Selective use of TBP and TFIIB revealed by a TATA-TBP-TFIIB array with altered specificity. Science. 1997 Feb 2/7/1997; 275(5301): 829-31. PMID: 9012349, ISSN: 0036-8075.
Pendergrast PS, Morrison D, Tansey WP, Hernandez N. Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly. J. Virol. 1996 Aug; 70(8): 5025-34. PMID: 8764009, PMCID: PMC190456, ISSN: 0022-538X.
Tansey WP, Herr W. The ability to associate with activation domains in vitro is not required for the TATA box-binding protein to support activated transcription in vivo. Proc. Natl. Acad. Sci. U.S.A. 1995 Nov 11/7/1995; 92(23): 10550-4. PMID: 7479838, PMCID: PMC40649, ISSN: 0027-8424.
Tansey WP, Ruppert S, Tjian R, Herr W. Multiple regions of TBP participate in the response to transcriptional activators in vivo. Genes Dev. 1994 Nov 11/15/1994; 8(22): 2756-69. PMID: 7958931, ISSN: 0890-9369.
Gilbert MT, Sun J, Yan Y, Oddoux C, Lazarus A, Tansey WP, Lavin TN, Catanzaro DF. Renin gene promoter activity in GC cells is regulated by cAMP and thyroid hormone through Pit-1-dependent mechanisms. J. Biol. Chem. 1994 Nov 11/11/1994; 269(45): 28049-54. PMID: 7961740, ISSN: 0021-9258.
Tansey WP, Schaufele F, Heslewood M, Handford C, Reudelhuber TL, Catanzaro DF. Distance-dependent interactions between basal, cyclic AMP, and thyroid hormone response elements in the rat growth hormone promoter. J. Biol. Chem. 1993 Jul 7/15/1993; 268(20): 14906-11. PMID: 8392063, ISSN: 0021-9258.
Henderson BR, Tansey WP, Phillips SM, Ramshaw IA, Kefford RF. Transcriptional and posttranscriptional activation of urokinase plasminogen activator gene expression in metastatic tumor cells. Cancer Res. 1992 May 5/1/1992; 52(9): 2489-96. PMID: 1568219, ISSN: 0008-5472.
Tansey WP, Catanzaro DF. Sp1 and thyroid hormone receptor differentially activate expression of human growth hormone and chorionic somatomammotropin genes. J. Biol. Chem. 1991 May 5/25/1991; 266(15): 9805-13. PMID: 2033067, ISSN: 0021-9258.
Available Postdoctoral Position Details
Posted: 9/18/2013
Postdoctoral fellows wanted to study basic mechanisms of MYC function and ways to inhibit them in cancer cells. See www.tanseylab.com for more details.