Daniel S. Perrien, Ph.D.

Assistant Professor


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Faculty Appointments
Assistant Professor of Medicine
Ph.D., University of Arkansas for Medical Sciences, Little Rock, ArkansasB.A., Hendrix College, Conway, Arkansas
Office Address
Light Hall/MRB4 Rm 1225E
2215-b Garland Ave
Nashville , TN 37232
Research Description
Dr. Perrien joined the Vanderbilt Center for Bone Biology in November 2008 and the Department of Orthopaedics and Rehabilitation in August of 2009. He also administers the microCT and other x-ray based instruments in the VUIIS. After receiving his Doctorate in Physiology and Biophysics from the University of Arkansas for Medical Sciences, he worked for an orthopaedic tissue engineering company, developing growth factor based drug/device therapies to enhance musculoskeletal repair. His lab now focuses on developing therapeutics and discovering new therapeutic targets for heterotopic ossification and a rare disease, fibrodysplasia ossificans progressiva. In addition, his efforts include mathematical modeling of structure-function relationships in bone, understanding the skeletal response to mechanical load, and endocrine control of bone mass and turnover.
Research Keywords
Fibrodysplasia ossificans progressiva, Heterotopic Ossification, Alk2, BMP, Bone Physiology, Fracture Repair, Distraction Osteogenesis, Imaging, Adaptation to Mechanical Load, Endocrinology, Sirtuin, TGFbRIII, betaglycan, TNF-alpha, microCT, Imaging
Lima GM, Corazza BJ, Moraes RM, de Oliveira FE, de Oliveira LD, Franco GC, Perrien DS, Elefteriou F, Anbinder AL. The effect of an inhibitor of gut serotonin (LP533401) during the induction of periodontal disease. J. Periodont. Res [print-electronic]. 2016 Oct; 51(5): 661-8. PMID: 26740292, DOI: 10.1111/jre.12346, ISSN: 1600-0765.

Uppuganti S, Granke M, Manhard MK, Does MD, Perrien DS, Lee DH, Nyman JS. Differences in sensitivity to microstructure between cyclic- and impact-based microindentation of human cortical bone. J. Orthop. Res [print-electronic]. 2016 Aug 8/11/2016; PMID: 27513922, DOI: 10.1002/jor.23392, ISSN: 1554-527X.

Perrien DS, Saleh MA, Takahashi K, Madhur MS, Harrison DG, Harris RC, Takahashi T. Novel methods for microCT-based analyses of vasculature in the renal cortex reveal a loss of perfusable arterioles and glomeruli in eNOS-/- mice. BMC Nephrol. 2016 Mar 3/2/2016; 17: 24. PMID: 26936597, PMCID: PMC4776352, PII: 10.1186/s12882-016-0235-5, DOI: 10.1186/s12882-016-0235-5, ISSN: 1471-2369.

Vignaux G, Ndong JD, Perrien DS, Elefteriou F. Inner Ear Vestibular Signals Regulate Bone Remodeling via the Sympathetic Nervous System. J. Bone Miner. Res. 2015 Jun; 30(6): 1103-11. PMID: 25491117, PMCID: PMC4772960, DOI: 10.1002/jbmr.2426, ISSN: 1523-4681.

de la Croix Ndong J, Stevens DM, Vignaux G, Uppuganti S, Perrien DS, Yang X, Nyman JS, Harth E, Elefteriou F. Combined MEK inhibition and BMP2 treatment promotes osteoblast differentiation and bone healing in Nf1Osx -/- mice. J. Bone Miner. Res. 2015 Jan; 30(1): 55-63. PMID: 25043591, PMCID: PMC4280331, DOI: 10.1002/jbmr.2316, ISSN: 1523-4681.

Nyman JS, Uppuganti S, Makowski AJ, Rowland BJ, Merkel AR, Sterling JA, Bredbenner TL, Perrien DS. Predicting mouse vertebra strength with micro-computed tomography-derived finite element analysis. Bonekey Rep. 2015; 4: 664. PMID: 25908967, PMCID: PMC4407510, DOI: 10.1038/bonekey.2015.31.

de la Croix Ndong J, Makowski AJ, Uppuganti S, Vignaux G, Ono K, Perrien DS, Joubert S, Baglio SR, Granchi D, Stevenson DA, Rios JJ, Nyman JS, Elefteriou F. Asfotase-a improves bone growth, mineralization and strength in mouse models of neurofibromatosis type-1. Nat. Med [print-electronic]. 2014 Aug; 20(8): 904-10. PMID: 24997609, PMCID: PMC4126855, PII: nm.3583, DOI: 10.1038/nm.3583, ISSN: 1546-170X.

Ding H, Nyman JS, Sterling JA, Perrien DS, Mahadevan-Jansen A, Bi X. Development of Raman spectral markers to assess metastatic bone in breast cancer. J Biomed Opt. 2014; 19(11): 111606. PMID: 24933683, PMCID: PMC4059340, PII: 1882987, DOI: 10.1117/1.JBO.19.11.111606, ISSN: 1560-2281.

Bi X, Sterling JA, Merkel AR, Perrien DS, Nyman JS, Mahadevan-Jansen A. Prostate cancer metastases alter bone mineral and matrix composition independent of effects on bone architecture in mice--a quantitative study using microCT and Raman spectroscopy. Bone [print-electronic]. 2013 Oct; 56(2): 454-60. PMID: 23867219, PMCID: PMC3799839, PII: S8756-3282(13)00266-4, DOI: 10.1016/j.bone.2013.07.006, ISSN: 1873-2763.

Cassat JE, Hammer ND, Campbell JP, Benson MA, Perrien DS, Mrak LN, Smeltzer MS, Torres VJ, Skaar EP. A secreted bacterial protease tailors the Staphylococcus aureus virulence repertoire to modulate bone remodeling during osteomyelitis. Cell Host Microbe. 2013 Jun 6/12/2013; 13(6): 759-72. PMID: 23768499, PMCID: PMC3721972, PII: S1931-3128(13)00185-6, DOI: 10.1016/j.chom.2013.05.003, ISSN: 1934-6069.

Edwards JR, Perrien DS, Fleming N, Nyman JS, Ono K, Connelly L, Moore MM, Lwin ST, Yull FE, Mundy GR, Elefteriou F. Silent information regulator (Sir)T1 inhibits NF-¿B signaling to maintain normal skeletal remodeling. J. Bone Miner. Res. 2013 Apr; 28(4): 960-9. PMID: 23172686, DOI: 10.1002/jbmr.1824, ISSN: 1523-4681.

Kawai VK, Stein CM, Perrien DS, Griffin MR. Effects of anti-tumor necrosis factor a agents on bone. Curr Opin Rheumatol. 2012 Sep; 24(5): 576-85. PMID: 22810364, PMCID: PMC3753172, DOI: 10.1097/BOR.0b013e328356d212, ISSN: 1531-6963.

Zhong J, Perrien DS, Yang HC, Kon V, Fogo AB, Ichikawa I, Ma J. Maturational regression of glomeruli determines the nephron population in normal mice. Pediatr. Res [print-electronic]. 2012 Sep; 72(3): 241-8. PMID: 22717689, PMCID: PMC3605193, PII: pr201281, DOI: 10.1038/pr.2012.81, ISSN: 1530-0447.

Campbell JP, Karolak MR, Ma Y, Perrien DS, Masood-Campbell SK, Penner NL, Munoz SA, Zijlstra A, Yang X, Sterling JA, Elefteriou F. Stimulation of host bone marrow stromal cells by sympathetic nerves promotes breast cancer bone metastasis in mice. PLoS Biol [print-electronic]. 2012 Jul; 10(7): e1001363. PMID: 22815651, PMCID: PMC3398959, PII: PBIOLOGY-D-12-00249, DOI: 10.1371/journal.pbio.1001363, ISSN: 1545-7885.

Johnson JA, Hemnes AR, Perrien DS, Schuster M, Robinson LJ, Gladson S, Loibner H, Bai S, Blackwell TR, Tada Y, Harral JW, Talati M, Lane KB, Fagan KA, West J. Cytoskeletal defects in Bmpr2-associated pulmonary arterial hypertension. Am. J. Physiol. Lung Cell Mol. Physiol [print-electronic]. 2012 Mar 3/1/2012; 302(5): L474-84. PMID: 22180660, PMCID: PMC3311512, PII: ajplung.00202.2011, DOI: 10.1152/ajplung.00202.2011, ISSN: 1522-1504.

Perrien DS, Nicks KM, Liu L, Akel NS, Bacon AW, Skinner RA, Swain FL, Aronson J, Suva LJ, Gaddy D. Inhibin A enhances bone formation during distraction osteogenesis. J. Orthop. Res [print-electronic]. 2012 Feb; 30(2): 288-95. PMID: 21809377, PMCID: PMC3737578, DOI: 10.1002/jor.21501, ISSN: 1554-527X.

Yu SS, Koblin RL, Zachman AL, Perrien DS, Hofmeister LH, Giorgio TD, Sung HJ. Physiologically relevant oxidative degradation of oligo(proline) cross-linked polymeric scaffolds. Biomacromolecules [print-electronic]. 2011 Dec 12/12/2011; 12(12): 4357-66. PMID: 22017359, PMCID: PMC3237771, DOI: 10.1021/bm201328k, ISSN: 1526-4602.

Brown KV, Li B, Guda T, Perrien DS, Guelcher SA, Wenke JC. Improving bone formation in a rat femur segmental defect by controlling bone morphogenetic protein-2 release. Tissue Eng Part A [print-electronic]. 2011 Jul; 17(13-14): 1735-46. PMID: 21338268, DOI: 10.1089/ten.TEA.2010.0446, ISSN: 1937-335X.

Nyman JS, Lynch CC, Perrien DS, Thiolloy S, O'Quinn EC, Patil CA, Bi X, Pharr GM, Mahadevan-Jansen A, Mundy GR. Differential effects between the loss of MMP-2 and MMP-9 on structural and tissue-level properties of bone. J. Bone Miner. Res. 2011 Jun; 26(6): 1252-60. PMID: 21611966, PMCID: PMC3312757, DOI: 10.1002/jbmr.326, ISSN: 1523-4681.

Sparks EE, Perrien DS, Huppert KA, Peterson TE, Huppert SS. Defects in hepatic Notch signaling result in disruption of the communicating intrahepatic bile duct network in mice. Dis Model Mech [print-electronic]. 2011 May; 4(3): 359-67. PMID: 21282722, PMCID: PMC3097457, PII: dmm.005793, DOI: 10.1242/dmm.005793, ISSN: 1754-8411.

Biswas S, Nyman JS, Alvarez J, Chakrabarti A, Ayres A, Sterling J, Edwards J, Rana T, Johnson R, Perrien DS, Lonning S, Shyr Y, Matrisian LM, Mundy GR. Anti-transforming growth factor ß antibody treatment rescues bone loss and prevents breast cancer metastasis to bone. PLoS ONE [print-electronic]. 2011; 6(11): e27090. PMID: 22096521, PMCID: PMC3214031, PII: PONE-D-11-14957, DOI: 10.1371/journal.pone.0027090, ISSN: 1932-6203.

Dumas JE, Davis T, Holt GE, Yoshii T, Perrien DS, Nyman JS, Boyce T, Guelcher SA. Synthesis, characterization, and remodeling of weight-bearing allograft bone/polyurethane composites in the rabbit. Acta Biomater [print-electronic]. 2010 Jul; 6(7): 2394-406. PMID: 20109586, PII: S1742-7061(10)00041-3, DOI: 10.1016/j.actbio.2010.01.030, ISSN: 1878-7568.

Nicks KM, Fowler TW, Akel NS, Perrien DS, Suva LJ, Gaddy D. Bone turnover across the menopause transition : The role of gonadal inhibins. Ann. N. Y. Acad. Sci. 2010 Mar; 1192: 153-60. PMID: 20392231, PMCID: PMC2964283, PII: NYAS5349, DOI: 10.1111/j.1749-6632.2009.05349.x, ISSN: 1749-6632.

Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, Yang X. Atf4 regulates chondrocyte proliferation and differentiation during endochondral ossification by activating Ihh transcription. Development [print-electronic]. 2009 Dec; 136(24): 4143-53. PMID: 19906842, PMCID: PMC2781050, PII: dev.043281, DOI: 10.1242/dev.043281, ISSN: 1477-9129.

Nicks KM, Perrien DS, Akel NS, Suva LJ, Gaddy D. Regulation of osteoblastogenesis and osteoclastogenesis by the other reproductive hormones, Activin and Inhibin. Mol. Cell. Endocrinol [print-electronic]. 2009 Oct 10/30/2009; 310(1-2): 11-20. PMID: 19615428, PMCID: PMC2951729, PII: S0303-7207(09)00358-X, DOI: 10.1016/j.mce.2009.07.001, ISSN: 1872-8057.

Fowlkes JL, Bunn RC, Liu L, Wahl EC, Coleman HN, Cockrell GE, Perrien DS, Lumpkin CK, Thrailkill KM. Runt-related transcription factor 2 (RUNX2) and RUNX2-related osteogenic genes are down-regulated throughout osteogenesis in type 1 diabetes mellitus. Endocrinology [print-electronic]. 2008 Apr; 149(4): 1697-704. PMID: 18162513, PMCID: PMC2276714, PII: en.2007-1408, DOI: 10.1210/en.2007-1408, ISSN: 0013-7227.

Shankar K, Hidestrand M, Liu X, Chen JR, Haley R, Perrien DS, Skinner RA, Lumpkin CK, Badger TM, Ronis MJ. Chronic ethanol consumption inhibits postlactational anabolic bone rebuilding in female rats. J. Bone Miner. Res. 2008 Mar; 23(3): 338-49. PMID: 17967133, DOI: 10.1359/jbmr.071023, ISSN: 1523-4681.

Wahl EC, Aronson J, Liu L, Liu Z, Perrien DS, Skinner RA, Badger TM, Ronis MJ, Lumpkin CK. Chronic ethanol exposure inhibits distraction osteogenesis in a mouse model: role of the TNF signaling axis. Toxicol. Appl. Pharmacol [print-electronic]. 2007 May 5/1/2007; 220(3): 302-10. PMID: 17391719, PMCID: PMC1892174, PII: S0041-008X(07)00073-7, DOI: 10.1016/j.taap.2007.02.011, ISSN: 0041-008X.

Perrien DS, Akel NS, Edwards PK, Carver AA, Bendre MS, Swain FL, Skinner RA, Hogue WR, Nicks KM, Pierson TM, Suva LJ, Gaddy D. Inhibin A is an endocrine stimulator of bone mass and strength. Endocrinology [print-electronic]. 2007 Apr; 148(4): 1654-65. PMID: 17194739, PII: en.2006-0848, DOI: 10.1210/en.2006-0848, ISSN: 0013-7227.

Liu Z, Aronson J, Wahl EC, Liu L, Perrien DS, Kern PA, Fowlkes JL, Thrailkill KM, Bunn RC, Cockrell GE, Skinner RA, Lumpkin CK. A novel rat model for the study of deficits in bone formation in type-2 diabetes. Acta Orthop. 2007 Feb; 78(1): 46-55. PMID: 17453392, PII: 776344008, DOI: 10.1080/17453670610013411, ISSN: 1745-3674.

Perrien DS, Akel NS, Dupont-Versteegden EE, Skinner RA, Siegel ER, Suva LJ, Gaddy D. Aging alters the skeletal response to disuse in the rat. Am. J. Physiol. Regul. Integr. Comp. Physiol [print-electronic]. 2007 Feb; 292(2): R988-96. PMID: 17068163, PII: 00302.2006, DOI: 10.1152/ajpregu.00302.2006, ISSN: 0363-6119.

Fowlkes JL, Thrailkill KM, Liu L, Wahl EC, Bunn RC, Cockrell GE, Perrien DS, Aronson J, Lumpkin CK. Effects of systemic and local administration of recombinant human IGF-I (rhIGF-I) on de novo bone formation in an aged mouse model. J. Bone Miner. Res. 2006 Sep; 21(9): 1359-66. PMID: 16939394, PMCID: PMC2424402, DOI: 10.1359/jbmr.060618, ISSN: 0884-0431.

Wahl EC, Liu L, Perrien DS, Aronson J, Hogue WR, Skinner RA, Hidestrand M, Ronis MJ, Badger TM, Lumpkin CK. A novel mouse model for the study of the inhibitory effects of chronic ethanol exposure on direct bone formation. Alcohol [print-electronic]. 2006 Jul; 39(3): 159-67. PMID: 17127135, PII: S0741-8329(06)00168-6, DOI: 10.1016/j.alcohol.2006.08.004, ISSN: 0741-8329.

Perrien DS, Achenbach SJ, Bledsoe SE, Walser B, Suva LJ, Khosla S, Gaddy D. Bone turnover across the menopause transition: correlations with inhibins and follicle-stimulating hormone. J. Clin. Endocrinol. Metab [print-electronic]. 2006 May; 91(5): 1848-54. PMID: 16449331, PII: jc.2005-2423, DOI: 10.1210/jc.2005-2423, ISSN: 0021-972X.

Thrailkill KM, Liu L, Wahl EC, Bunn RC, Perrien DS, Cockrell GE, Skinner RA, Hogue WR, Carver AA, Fowlkes JL, Aronson J, Lumpkin CK. Bone formation is impaired in a model of type 1 diabetes. Diabetes. 2005 Oct; 54(10): 2875-81. PMID: 16186388, PII: 54/10/2875, ISSN: 0012-1797.

Wahl EC, Perrien DS, Aronson J, Liu Z, Fletcher TW, Skinner RA, Feige U, Suva LJ, Badger TM, Lumpkin CK. Ethanol-induced inhibition of bone formation in a rat model of distraction osteogenesis: a role for the tumor necrosis factor signaling axis. Alcohol. Clin. Exp. Res. 2005 Aug; 29(8): 1466-72. PMID: 16131855, PII: 00000374-200508000-00014, ISSN: 0145-6008.

Suva LJ, Gaddy D, Perrien DS, Thomas RL, Findlay DM. Regulation of bone mass by mechanical loading: microarchitecture and genetics. Curr Osteoporos Rep. 2005 Jun; 3(2): 46-51. PMID: 16036101, ISSN: 1544-1873.

Perrien DS, Wahl EC, Hogue WR, Feige U, Aronson J, Ronis MJ, Badger TM, Lumpkin CK. IL-1 and TNF antagonists prevent inhibition of fracture healing by ethanol in rats. Toxicol. Sci [print-electronic]. 2004 Dec; 82(2): 656-60. PMID: 15470231, PII: kfi002, DOI: 10.1093/toxsci/kfi002, ISSN: 1096-6080.

Perrien DS, Liu Z, Wahl EC, Bunn RC, Skinner RA, Aronson J, Fowlkes J, Badger TM, Lumpkin CK. Chronic ethanol exposure is associated with a local increase in TNF-alpha and decreased proliferation in the rat distraction gap. Cytokine. 2003 Sep 9/21/2003; 23(6): 179-89. PMID: 12967643, PII: S1043466603002254, ISSN: 1043-4666.

Perrien DS, Brown EC, Fletcher TW, Irby DJ, Aronson J, Gao GG, Skinner RA, Hogue WR, Feige U, Suva LJ, Ronis MJ, Badger TM, Lumpkin CK. Interleukin-1 and tumor necrosis factor antagonists attenuate ethanol-induced inhibition of bone formation in a rat model of distraction osteogenesis. J. Pharmacol. Exp. Ther. 2002 Dec; 303(3): 904-8. PMID: 12438508, DOI: 10.1124/jpet.102.039636, ISSN: 0022-3565.

Brown EC, Perrien DS, Fletcher TW, Irby DJ, Aronson J, Gao GG, Hogue WJ, Skinner RA, Suva LJ, Ronis MJ, Hakkak R, Badger TM, Lumpkin CK. Skeletal toxicity associated with chronic ethanol exposure in a rat model using total enteral nutrition. J. Pharmacol. Exp. Ther. 2002 Jun; 301(3): 1132-8. PMID: 12023547, ISSN: 0022-3565.

Perrien DS, Brown EC, Aronson J, Skinner RA, Montague DC, Badger TM, Lumpkin CK. Immunohistochemical study of osteopontin expression during distraction osteogenesis in the rat. J. Histochem. Cytochem. 2002 Apr; 50(4): 567-74. PMID: 11897810, ISSN: 0022-1554.