Imaging effectively offers a ‘blueprint’ as to how biological components work together by providing spatial context to molecular information. From the advent of the complex microscope in the late 1600s to modern modalities such as magnetic resonance, positron emission tomography, and advanced microscopy, imaging technologies have always been at the forefront of our understanding of biochemistry and biology. However, relative to the new -omics technologies, these classical biomedical imaging technologies have limited molecular specificity. Mass spectrometry-based imaging now finds itself uniquely positioned to bridge the gap between the information rich genomics, proteomics, and metabolomics approaches and biomedical imaging technologies. IMS combines the molecular specificity of mass spectrometry with the spatial fidelity of classical histology to create molecular maps of tissues. Broadly, my research falls into two categories: (1) Developing novel mass spectrometry technologies to maximize imaging performance enabling molecular histology at cellular resolution and (2) combining imaging mass spectrometry with a variety of other biomedical imaging technologies to create new integrated modalities capable of providing a systems biology view of tissue at cellular resolution.
Perry WJ, Grunenwald CM, Van de Plas R, Witten JC, Martin DR, Apte SS, Cassat JE, Pettersson GB, Caprioli RM, Skaar EP, Spraggins JM. Visualizing Staphylococcus aureus pathogenic membrane modification within the host infection environment by multimodal imaging mass spectrometry. Cell Chem Biol [print-electronic]. 2022 Jul 7/21/2022; 29(7): 1209-1217.e4. PMID: 35654040, PMCID: PMC9308753, PII: S2451-9456(22)00196-9, DOI: 10.1016/j.chembiol.2022.05.004, ISSN: 2451-9448.
Sharman K, Patterson NH, Weiss A, Neumann EK, Guiberson ER, Ryan DJ, Gutierrez DB, Spraggins JM, Van de Plas R, Skaar EP, Caprioli RM. Rapid Multivariate Analysis Approach to Explore Differential Spatial Protein Profiles in Tissue. J Proteome Res [print-electronic]. 2022 Jul 7/18/2022; PMID: 35849531, DOI: 10.1021/acs.jproteome.2c00206, ISSN: 1535-3907.
Burnum-Johnson KE, Conrads TP, Drake RR, Herr AE, Iyengar R, Kelly RT, Lundberg E, MacCoss MJ, Naba A, Nolan GP, Pevzner PA, Rodland KD, Sechi S, Slavov N, Spraggins JM, Van Eyk JE, Vidal M, Vogel C, Walt DR, Kelleher NL. New Views of Old Proteins: Clarifying the Enigmatic Proteome. Mol Cell Proteomics [print-electronic]. 2022 Jul; 21(7): 100254. PMID: 35654359, PMCID: PMC9256833, PII: S1535-9476(22)00062-7, DOI: 10.1016/j.mcpro.2022.100254, ISSN: 1535-9484.
Lee HH, Tang Y, Xu K, Bao S, Fogo AB, Harris R, de Caestecker MP, Heinrich M, Spraggins JM, Huo Y, Landman BA. Multi-contrast computed tomography healthy kidney atlas. Comput Biol Med [print-electronic]. 2022 Jul; 146: 105555. PMID: 35533459, PII: S0010-4825(22)00347-X, DOI: 10.1016/j.compbiomed.2022.105555, ISSN: 1879-0534.
Weiss A, Murdoch CC, Edmonds KA, Jordan MR, Monteith AJ, Perera YR, Rodríguez Nassif AM, Petoletti AM, Beavers WN, Munneke MJ, Drury SL, Krystofiak ES, Thalluri K, Wu H, Kruse ARS, DiMarchi RD, Caprioli RM, Spraggins JM, Chazin WJ, Giedroc DP, Skaar EP. Zn-regulated GTPase metalloprotein activator 1 modulates vertebrate zinc homeostasis. Cell [print-electronic]. 2022 Jun 6/9/2022; 185(12): 2148-2163.e27. PMID: 35584702, PMCID: PMC9189065, PII: S0092-8674(22)00458-5, DOI: 10.1016/j.cell.2022.04.011, ISSN: 1097-4172.
Guiberson ER, Good CJ, Wexler AG, Skaar EP, Spraggins JM, Caprioli RM. Multimodal Imaging Mass Spectrometry of Murine Gastrointestinal Tract with Retained Luminal Content. J Am Soc Mass Spectrom [print-electronic]. 2022 Jun 6/1/2022; 33(6): 1073-6. PMID: 35545232, PMCID: PMC9264265, DOI: 10.1021/jasms.1c00360, ISSN: 1879-1123.
Manz T, Gold I, Patterson NH, McCallum C, Keller MS, Herr BW, Börner K, Spraggins JM, Gehlenborg N. Viv: multiscale visualization of high-resolution multiplexed bioimaging data on the web [letter]. Nat Methods. 2022 May; 19(5): 515-6. PMID: 35545714, PMCID: PMC9637380, PII: 10.1038/s41592-022-01482-7, DOI: 10.1038/s41592-022-01482-7, ISSN: 1548-7105.
Richardson LT, Neumann EK, Caprioli RM, Spraggins JM, Solouki T. Referenced Kendrick Mass Defect Annotation and Class-Based Filtering of Imaging MS Lipidomics Experiments. Anal Chem [print-electronic]. 2022 Apr 4/12/2022; 94(14): 5504-13. PMID: 35344335, DOI: 10.1021/acs.analchem.1c03715, ISSN: 1520-6882.
Good CJ, Neumann EK, Butrico CE, Cassat JE, Caprioli RM, Spraggins JM. High Spatial Resolution MALDI Imaging Mass Spectrometry of Fresh-Frozen Bone. Anal Chem [print-electronic]. 2022 Feb 2/22/2022; 94(7): 3165-72. PMID: 35138834, DOI: 10.1021/acs.analchem.1c04604, ISSN: 1520-6882.
Lee HH, Tang Y, Bao S, Yang Q, Xu X, Fogo AB, Harris R, de Caestecker MP, Spraggins JM, Heinrich M, Huo Y, Landman BA. Supervised Deep Generation of High-Resolution Arterial Phase Computed Tomography Kidney Substructure Atlas. Proc SPIE Int Soc Opt Eng [print-electronic]. 2022 Feb; 12032: PMID: 36303577, PMCID: PMC9605120, DOI: 10.1117/12.2608290, ISSN: 0277-786X.
Neumann EK, Patterson NH, Rivera ES, Allen JL, Brewer M, deCaestecker MP, Caprioli RM, Fogo AB, Spraggins JM. Highly multiplexed immunofluorescence of the human kidney using co-detection by indexing. Kidney Int [print-electronic]. 2022 Jan; 101(1): 137-43. PMID: 34619231, PMCID: PMC8741652, PII: S0085-2538(21)00923-6, DOI: 10.1016/j.kint.2021.08.033, ISSN: 1523-1755.
Kruse ARS, Spraggins JM. Uncovering Molecular Heterogeneity in the Kidney With Spatially Targeted Mass Spectrometry. Front Physiol. 2022; 13: 837773. PMID: 35222094, PMCID: PMC8874197, DOI: 10.3389/fphys.2022.837773, ISSN: 1664-042X.
Hickey JW, Neumann EK, Radtke AJ, Camarillo JM, Beuschel RT, Albanese A, McDonough E, Hatler J, Wiblin AE, Fisher J, Croteau J, Small EC, Sood A, Caprioli RM, Angelo RM, Nolan GP, Chung K, Hewitt SM, Germain RN, Spraggins JM, Lundberg E, Snyder MP, Kelleher NL, Saka SK. Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging. Nat Methods [print-electronic]. 2021 Nov 11/22/2021; PMID: 34811556, PII: 10.1038/s41592-021-01316-y, DOI: 10.1038/s41592-021-01316-y, ISSN: 1548-7105.
McMillen JC, Gutierrez DB, Judd AM, Spraggins JM, Caprioli RM. Enhancement of Tryptic Peptide Signals from Tissue Sections Using MALDI IMS Postionization (MALDI-2). J Am Soc Mass Spectrom [print-electronic]. 2021 Oct 10/6/2021; 32(10): 2583-91. PMID: 34515472, DOI: 10.1021/jasms.1c00213, ISSN: 1879-1123.
Neumann EK, Patterson NH, Allen JL, Migas LG, Yang H, Brewer M, Anderson DM, Harvey J, Gutierrez DB, Harris RC, deCaestecker MP, Fogo AB, Van de Plas R, Caprioli RM, Spraggins JM. Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence. STAR Protoc. 2021 Sep 9/17/2021; 2(3): 100747. PMID: 34430920, PMCID: PMC8371244, PII: S2666-1667(21)00454-8, DOI: 10.1016/j.xpro.2021.100747, ISSN: 2666-1667.
Tideman LEM, Migas LG, Djambazova KV, Patterson NH, Caprioli RM, Spraggins JM, Van de Plas R. Automated biomarker candidate discovery in imaging mass spectrometry data through spatially localized Shapley additive explanations. Anal Chim Acta [print-electronic]. 2021 Sep 9/8/2021; 1177: 338522. PMID: 34482894, PII: S0003-2670(21)00348-2, DOI: 10.1016/j.aca.2021.338522, ISSN: 1873-4324.
Tideman LEM, Migas LG, Djambazova KV, Patterson NH, Caprioli RM, Spraggins JM, Van de Plas R. Automated biomarker candidate discovery in imaging mass spectrometry data through spatially localized Shapley additive explanations. Anal Chim Acta [print-electronic]. 2021 Sep 9/8/2021; 1177: 338522. PMID: 34482894, PII: S0003-2670(21)00348-2, DOI: 10.1016/j.aca.2021.338522, ISSN: 1873-4324.
Wexler AG, Guiberson ER, Beavers WN, Shupe JA, Washington MK, Lacy DB, Caprioli RM, Spraggins JM, Skaar EP. Clostridioides difficile infection induces a rapid influx of bile acids into the gut during colonization of the host. Cell Rep. 2021 Sep 9/7/2021; 36(10): 109683. PMID: 34496241, PMCID: PMC8445666, PII: S2211-1247(21)01130-X, DOI: 10.1016/j.celrep.2021.109683, ISSN: 2211-1247.
Dufresne M, Fincher JA, Patterson NH, Schey KL, Norris JL, Caprioli RM, Spraggins JM. A-Cyano-4-hydroxycinnamic Acid and Tri-Potassium Citrate Salt Pre-Coated Silicon Nanopost Array Provides Enhanced Lipid Detection for High Spatial Resolution MALDI Imaging Mass Spectrometry. Anal Chem [print-electronic]. 2021 Aug 8/27/2021; PMID: 34449196, DOI: 10.1021/acs.analchem.1c01560, ISSN: 1520-6882.
Fincher JA, Djambazova KV, Klein DR, Dufresne M, Migas LG, Van de Plas R, Caprioli RM, Spraggins JM. Molecular Mapping of Neutral Lipids Using Silicon Nanopost Arrays and TIMS Imaging Mass Spectrometry. J Am Soc Mass Spectrom [print-electronic]. 2021 Aug 8/26/2021; PMID: 34435768, DOI: 10.1021/jasms.1c00159, ISSN: 1879-1123.
Guiberson ER, Weiss A, Ryan DJ, Monteith AJ, Sharman K, Gutierrez DB, Perry WJ, Caprioli RM, Skaar EP, Spraggins JM. Spatially Targeted Proteomics of the Host-Pathogen Interface during Staphylococcal Abscess Formation. ACS Infect Dis [print-electronic]. 2021 Jan 1/8/2021; 7(1): 101-13. PMID: 33270421, PMCID: PMC7796985, DOI: 10.1021/acsinfecdis.0c00647, ISSN: 2373-8227.
Lee HH, Tang Y, Xu K, Bao S, Fogo AB, Harris R, de Caestecker MP, Heinrich M, Spraggins JM, Huo Y, Landman BA. Construction of a Multi-Phase Contrast Computed Tomography Kidney Atlas. Proc SPIE Int Soc Opt Eng [print-electronic]. 2021; 11596: PMID: 34354322, PMCID: PMC8336653, DOI: 10.1117/12.2580561, ISSN: 0277-786X.
Lee HH, Tang Y, Xu K, Bao S, Fogo AB, Harris R, de Caestecker MP, Heinrich M, Spraggins JM, Huo Y, Landman BA. Construction of a Multi-Phase Contrast Computed Tomography Kidney Atlas. Proc SPIE Int Soc Opt Eng [print-electronic]. 2021; 11596: PMID: 34354322, PMCID: PMC8336653, DOI: 10.1117/12.2580561, ISSN: 0277-786X.
Tang Y, Gao R, Lee HH, Xu Z, Savoie BV, Bao S, Huo Y, Fogo AB, Harris R, de Caestecker MP, Spraggins J, Landman BA. Renal Cortex, Medulla and Pelvicaliceal System Segmentation on Arterial Phase CT Images with Random Patch-based Networks. Proc SPIE Int Soc Opt Eng [print-electronic]. 2021; 11596: PMID: 34531632, PMCID: PMC8442958, DOI: 10.1117/12.2581101, ISSN: 0277-786X.
Neumann EK, Djambazova KV, Caprioli RM, Spraggins JM. Multimodal Imaging Mass Spectrometry: Next Generation Molecular Mapping in Biology and Medicine. J Am Soc Mass Spectrom [print-electronic]. 2020 Dec 12/2/2020; 31(12): 2401-15. PMID: 32886506, DOI: 10.1021/jasms.0c00232, ISSN: 1879-1123.
Li L, Laskin J, Spraggins J. 35th ASMS Asilomar Conference on Mass Spectrometry. Mass Spectrometry Imaging: New Developments and Applications [editorial]. J Am Soc Mass Spectrom. 2020 Dec 12/2/2020; 31(12): 2390-1. PMID: 33595332, DOI: 10.1021/jasms.0c00404, ISSN: 1879-1123.
Anderson DMG, Messinger JD, Patterson NH, Rivera ES, Kotnala A, Spraggins JM, Caprioli RM, Curcio CA, Schey KL. Lipid Landscape of the Human Retina and Supporting Tissues Revealed by High-Resolution Imaging Mass Spectrometry. J Am Soc Mass Spectrom [print-electronic]. 2020 Dec 12/2/2020; 31(12): 2426-36. PMID: 32628476, PMCID: PMC8161663, DOI: 10.1021/jasms.0c00119, ISSN: 1879-1123.
Rivera ES, Djambazova KV, Neumann EK, Caprioli RM, Spraggins JM. Integrating ion mobility and imaging mass spectrometry for comprehensive analysis of biological tissues: A brief review and perspective. J Mass Spectrom [print-electronic]. 2020 Dec; 55(12): e4614. PMID: 32955134, PMCID: PMC8211109, DOI: 10.1002/jms.4614, ISSN: 1096-9888.
McMillen JC, Fincher JA, Klein DR, Spraggins JM, Caprioli RM. Effect of MALDI matrices on lipid analyses of biological tissues using MALDI-2 postionization mass spectrometry. J Mass Spectrom. 2020 Dec; 55(12): e4663. PMID: 33241625, PMCID: PMC8099046, DOI: 10.1002/jms.4663, ISSN: 1096-9888.
Limbrick EM, Yñigez-Gutierrez AE, Dulin CC, Derewacz DK, Spraggins JM, McCulloch KM, Iverson TM, Bachmann BO. Methyltransferase Contingencies in the Pathway of Everninomicin D Antibiotics and Analogues. Chembiochem [print-electronic]. 2020 Dec 12/1/2020; 21(23): 3349-58. PMID: 32686210, DOI: 10.1002/cbic.202000305, ISSN: 1439-7633.
Limbrick EM, Graf M, Derewacz DK, Nguyen F, Spraggins JM, Wieland M, Ynigez-Gutierrez AE, Reisman BJ, Zinshteyn B, McCulloch KM, Iverson TM, Green R, Wilson DN, Bachmann BO. Bifunctional Nitrone-Conjugated Secondary Metabolite Targeting the Ribosome. J Am Chem Soc [print-electronic]. 2020 Oct 10/28/2020; 142(43): 18369-77. PMID: 32709196, PMCID: PMC8129991, DOI: 10.1021/jacs.0c04675, ISSN: 1520-5126.
Prentice BM, Ryan DJ, Grove KJ, Cornett DS, Caprioli RM, Spraggins JM. Dynamic Range Expansion by Gas-Phase Ion Fractionation and Enrichment for Imaging Mass Spectrometry. Anal Chem [print-electronic]. 2020 Oct 10/6/2020; 92(19): 13092-100. PMID: 32845133, PMCID: PMC8340028, DOI: 10.1021/acs.analchem.0c02121, ISSN: 1520-6882.
Djambazova KV, Klein DR, Migas LG, Neumann EK, Rivera ES, Van de Plas R, Caprioli RM, Spraggins JM. Resolving the Complexity of Spatial Lipidomics Using MALDI TIMS Imaging Mass Spectrometry. Anal Chem [print-electronic]. 2020 Oct 10/6/2020; 92(19): 13290-7. PMID: 32808523, DOI: 10.1021/acs.analchem.0c02520, ISSN: 1520-6882.
Neumann EK, Migas LG, Allen JL, Caprioli RM, Van de Plas R, Spraggins JM. Spatial Metabolomics of the Human Kidney using MALDI Trapped Ion Mobility Imaging Mass Spectrometry. Anal Chem [print-electronic]. 2020 Oct 10/6/2020; 92(19): 13084-91. PMID: 32668145, DOI: 10.1021/acs.analchem.0c02051, ISSN: 1520-6882.
Palmer LD, Minor KE, Mettlach JA, Rivera ES, Boyd KL, Caprioli RM, Spraggins JM, Dalebroux ZD, Skaar EP. Modulating Isoprenoid Biosynthesis Increases Lipooligosaccharides and Restores Acinetobacter baumannii Resistance to Host and Antibiotic Stress. Cell Rep. 2020 Sep 9/8/2020; 32(10): 108129. PMID: 32905776, PMCID: PMC7519801, PII: S2211-1247(20)31118-9, DOI: 10.1016/j.celrep.2020.108129, ISSN: 2211-1247.
Manzo T, Prentice BM, Anderson KG, Raman A, Schalck A, Codreanu GS, Nava Lauson CB, Tiberti S, Raimondi A, Jones MA, Reyzer M, Bates BM, Spraggins JM, Patterson NH, McLean JA, Rai K, Tacchetti C, Tucci S, Wargo JA, Rodighiero S, Clise-Dwyer K, Sherrod SD, Kim M, Navin NE, Caprioli RM, Greenberg PD, Draetta G, Nezi L. Accumulation of long-chain fatty acids in the tumor microenvironment drives dysfunction in intrapancreatic CD8+ T cells. J Exp Med. 2020 Aug 8/3/2020; 217(8): PMID: 32491160, PMCID: PMC7398173, PII: 151833, DOI: 10.1084/jem.20191920, ISSN: 1540-9538.
Islam M, Chen B, Spraggins JM, Kelly RT, Lau KS. Use of Single-Cell -Omic Technologies to Study the Gastrointestinal Tract and Diseases, From Single Cell Identities to Patient Features. Gastroenterology [print-electronic]. 2020 Aug; 159(2): 453-466.e1. PMID: 32417404, PMCID: PMC7484006, PII: S0016-5085(20)30656-9, DOI: 10.1053/j.gastro.2020.04.073, ISSN: 1528-0012.
Jones MA, Cho SH, Patterson NH, Van de Plas R, Spraggins JM, Boothby MR, Caprioli RM. Discovering New Lipidomic Features Using Cell Type Specific Fluorophore Expression to Provide Spatial and Biological Specificity in a Multimodal Workflow with MALDI Imaging Mass Spectrometry. Anal Chem [print-electronic]. 2020 May 5/19/2020; 92(10): 7079-86. PMID: 32298091, PMCID: PMC7456589, DOI: 10.1021/acs.analchem.0c00446, ISSN: 1520-6882.
Perry WJ, Weiss A, Van de Plas R, Spraggins JM, Caprioli RM, Skaar EP. Integrated molecular imaging technologies for investigation of metals in biological systems: A brief review. Curr Opin Chem Biol [print-electronic]. 2020 Apr; 55: 127-35. PMID: 32087551, PMCID: PMC7237308, PII: S1367-5931(20)30009-0, DOI: 10.1016/j.cbpa.2020.01.008, ISSN: 1879-0402.
Perry WJ, Patterson NH, Prentice BM, Neumann EK, Caprioli RM, Spraggins JM. Uncovering matrix effects on lipid analyses in MALDI imaging mass spectrometry experiments. J Mass Spectrom [print-electronic]. 2020 Apr; 55(4): e4491. PMID: 31860760, PMCID: PMC7383219, DOI: 10.1002/jms.4491, ISSN: 1096-9888.
Spraggins JM, Djambazova KV, Rivera ES, Migas LG, Neumann EK, Fuetterer A, Suetering J, Goedecke N, Ly A, Van de Plas R, Caprioli RM. High-Performance Molecular Imaging with MALDI Trapped Ion-Mobility Time-of-Flight (timsTOF) Mass Spectrometry. Anal Chem [print-electronic]. 2019 Nov 11/19/2019; 91(22): 14552-60. PMID: 31593446, PMCID: PMC7382025, DOI: 10.1021/acs.analchem.9b03612, ISSN: 1520-6882.
Perry WJ, Spraggins JM, Sheldon JR, Grunenwald CM, Heinrichs DE, Cassat JE, Skaar EP, Caprioli RM. Staphylococcus aureus exhibits heterogeneous siderophore production within the vertebrate host. Proc Natl Acad Sci U S A [print-electronic]. 2019 Oct 10/29/2019; 116(44): 21980-2. PMID: 31611408, PMCID: PMC6825271, PII: 1913991116, DOI: 10.1073/pnas.1913991116, ISSN: 1091-6490.
. The human body at cellular resolution: the NIH Human Biomolecular Atlas Program. Nature [print-electronic]. 2019 Oct; 574(7777): 187-92. PMID: 31597973, PMCID: PMC6800388, PII: 10.1038/s41586-019-1629-x, DOI: 10.1038/s41586-019-1629-x, ISSN: 1476-4687.
Ryan DJ, Patterson NH, Putnam NE, Wilde AD, Weiss A, Perry WJ, Cassat JE, Skaar EP, Caprioli RM, Spraggins JM. MicroLESA: Integrating Autofluorescence Microscopy, In Situ Micro-Digestions, and Liquid Extraction Surface Analysis for High Spatial Resolution Targeted Proteomic Studies. Anal Chem [print-electronic]. 2019 Jun 6/18/2019; 91(12): 7578-85. PMID: 31149808, PMCID: PMC6652190, DOI: 10.1021/acs.analchem.8b05889, ISSN: 1520-6882.
Prentice BM, Hart NJ, Phillips N, Haliyur R, Judd A, Armandala R, Spraggins JM, Lowe CL, Boyd KL, Stein RW, Wright CV, Norris JL, Powers AC, Brissova M, Caprioli RM. Imaging mass spectrometry enables molecular profiling of mouse and human pancreatic tissue. Diabetologia [print-electronic]. 2019 Jun; 62(6): 1036-47. PMID: 30955045, PMCID: PMC6553460, PII: 10.1007/s00125-019-4855-8, DOI: 10.1007/s00125-019-4855-8, ISSN: 1432-0428.
Gessel MM, Spraggins JM, Voziyan PA, Abrahamson DR, Caprioli RM, Hudson BG. Two Specific Sulfatide Species Are Dysregulated during Renal Development in a Mouse Model of Alport Syndrome. Lipids [print-electronic]. 2019 Jun; 54(6-7): 411-8. PMID: 31197846, PMCID: PMC6655412, DOI: 10.1002/lipd.12171, ISSN: 1558-9307.
Spraggins JM, Schwamborn K, Heeren RMA, Eberlin LS. The Importance of Clinical Tissue Imaging. Clin Mass Spectrom [print-electronic]. 2019 Apr; 12: 47-9. PMID: 32483555, PMCID: PMC7263479, DOI: 10.1016/j.clinms.2019.04.001, ISSN: 2376-9998.
Spivey EC, McMillen JC, Ryan DJ, Spraggins JM, Caprioli RM. Combining MALDI-2 and transmission geometry laser optics to achieve high sensitivity for ultra-high spatial resolution surface analysis. J Mass Spectrom. 2019 Apr; 54(4): 366-70. PMID: 30675932, DOI: 10.1002/jms.4335, ISSN: 1096-9888.
Ryan DJ, Spraggins JM, Caprioli RM. Protein identification strategies in MALDI imaging mass spectrometry: a brief review. Curr Opin Chem Biol [print-electronic]. 2019 Feb; 48: 64-72. PMID: 30476689, PMCID: PMC6382520, PII: S1367-5931(18)30130-3, DOI: 10.1016/j.cbpa.2018.10.023, ISSN: 1879-0402.
Cressman ENK, Spraggins JM. Viewing the Future of IR through Molecular Histology: An Overview of Imaging Mass Spectrometry. J Vasc Interv Radiol [print-electronic]. 2018 Nov; 29(11): 1543-1546.e1. PMID: 30274858, PMCID: PMC6800995, PII: S1051-0443(18)31328-9, DOI: 10.1016/j.jvir.2018.07.004, ISSN: 1535-7732.
Covington BC, Spraggins JM, Ynigez-Gutierrez AE, Hylton ZB, Bachmann BO. Response of Secondary Metabolism of Hypogean Actinobacterial Genera to Chemical and Biological Stimuli. Appl Environ Microbiol [electronic-print]. 2018 Oct 10/1/2018; 84(19): PMID: 30030223, PMCID: PMC6146984, PII: AEM.01125-18, DOI: 10.1128/AEM.01125-18, ISSN: 1098-5336.
Kleinridders A, Ferris HA, Reyzer ML, Rath M, Soto M, Manier ML, Spraggins J, Yang Z, Stanton RC, Caprioli RM, Kahn CR. Regional differences in brain glucose metabolism determined by imaging mass spectrometry. Mol Metab [print-electronic]. 2018 Jun; 12: 113-21. PMID: 29681509, PMCID: PMC6001904, PII: S2212-8778(18)30102-9, DOI: 10.1016/j.molmet.2018.03.013, ISSN: 2212-8778.
Prentice BM, Ryan DJ, Van de Plas R, Caprioli RM, Spraggins JM. Enhanced Ion Transmission Efficiency up to m/ z 24¿000 for MALDI Protein Imaging Mass Spectrometry. Anal Chem [print-electronic]. 2018 Apr 4/17/2018; 90(8): 5090-9. PMID: 29444410, PMCID: PMC6905630, DOI: 10.1021/acs.analchem.7b05105, ISSN: 1520-6882.
Ryan DJ, Nei D, Prentice BM, Rose KL, Caprioli RM, Spraggins JM. Protein identification in imaging mass spectrometry through spatially targeted liquid micro-extractions. Rapid Commun Mass Spectrom. 2018 Mar 3/15/2018; 32(5): 442-50. PMID: 29226434, PMCID: PMC5812809, DOI: 10.1002/rcm.8042, ISSN: 1097-0231.
Cassat JE, Moore JL, Wilson KJ, Stark Z, Prentice BM, Van de Plas R, Perry WJ, Zhang Y, Virostko J, Colvin DC, Rose KL, Judd AM, Reyzer ML, Spraggins JM, Grunenwald CM, Gore JC, Caprioli RM, Skaar EP. Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions. Sci Transl Med. 2018 Mar 3/14/2018; 10(432): PMID: 29540616, PMCID: PMC6005374, PII: 10/432/eaan6361, DOI: 10.1126/scitranslmed.aan6361, ISSN: 1946-6242.
Anderson DMG, Ablonczy Z, Koutalos Y, Hanneken AM, Spraggins JM, Calcutt MW, Crouch RK, Caprioli RM, Schey KL. Bis(monoacylglycero)phosphate lipids in the retinal pigment epithelium implicate lysosomal/endosomal dysfunction in a model of Stargardt disease and human retinas. Sci Rep. 2017 Dec 12/11/2017; 7(1): 17352. PMID: 29229934, PMCID: PMC5725462, PII: 10.1038/s41598-017-17402-1, DOI: 10.1038/s41598-017-17402-1, ISSN: 2045-2322.
Verbeeck N, Spraggins JM, Murphy MJM, Wang HD, Deutch AY, Caprioli RM, Van de Plas R. Connecting imaging mass spectrometry and magnetic resonance imaging-based anatomical atlases for automated anatomical interpretation and differential analysis. Biochim Biophys Acta Proteins Proteom [print-electronic]. 2017 Jul; 1865(7): 967-77. PMID: 28254588, PII: S1570-9639(17)30040-7, DOI: 10.1016/j.bbapap.2017.02.016, ISSN: 1570-9639.
Norris JL, Farrow MA, Gutierrez DB, Palmer LD, Muszynski N, Sherrod SD, Pino JC, Allen JL, Spraggins JM, Lubbock AL, Jordan A, Burns W, Poland JC, Romer C, Manier ML, Nei YW, Prentice BM, Rose KL, Hill S, Van de Plas R, Tsui T, Braman NM, Keller MR, Rutherford SA, Lobdell N, Lopez CF, Lacy DB, McLean JA, Wikswo JP, Skaar EP, Caprioli RM. Integrated, High-Throughput, Multiomics Platform Enables Data-Driven Construction of Cellular Responses and Reveals Global Drug Mechanisms of Action. J Proteome Res [print-electronic]. 2017 Mar 3/3/2017; 16(3): 1364-75. PMID: 28088864, DOI: 10.1021/acs.jproteome.6b01004, ISSN: 1535-3907.
Nye-Wood MG, Spraggins JM, Caprioli RM, Schey KL, Donaldson PJ, Grey AC. Spatial distributions of glutathione and its endogenous conjugates in normal bovine lens and a model of lens aging. Exp Eye Res [print-electronic]. 2017 Jan; 154: 70-8. PMID: 27838309, PMCID: PMC5359059, PII: S0014-4835(16)30442-0, DOI: 10.1016/j.exer.2016.11.008, ISSN: 1096-0007.
Zubair F, Laibinis PE, Swisher WG, Yang J, Spraggins JM, Norris JL, Caprioli RM. Trypsin and MALDI matrix pre-coated targets simplify sample preparation for mapping proteomic distributions within biological tissues by imaging mass spectrometry. J Mass Spectrom. 2016 Dec; 51(12): 1168-79. PMID: 27676701, PMCID: PMC5687832, DOI: 10.1002/jms.3888, ISSN: 1096-9888.
Spraggins JM, Rizzo DG, Moore JL, Noto MJ, Skaar EP, Caprioli RM. Next-generation technologies for spatial proteomics: Integrating ultra-high speed MALDI-TOF and high mass resolution MALDI FTICR imaging mass spectrometry for protein analysis. Proteomics [print-electronic]. 2016 Jun; 16(11-12): 1678-89. PMID: 27060368, PMCID: PMC5117945, DOI: 10.1002/pmic.201600003, ISSN: 1615-9861.
Marien E, Meister M, Muley T, Gomez Del Pulgar T, Derua R, Spraggins JM, Van de Plas R, Vanderhoydonc F, Machiels J, Binda MM, Dehairs J, Willette-Brown J, Hu Y, Dienemann H, Thomas M, Schnabel PA, Caprioli RM, Lacal JC, Waelkens E, Swinnen JV. Phospholipid profiling identifies acyl chain elongation as a ubiquitous trait and potential target for the treatment of lung squamous cell carcinoma. Oncotarget. 2016 Mar 3/15/2016; 7(11): 12582-97. PMID: 26862848, PMCID: PMC4914306, PII: 7179, DOI: 10.18632/oncotarget.7179, ISSN: 1949-2553.
de Macedo CS, Anderson DM, Pascarelli BM, Spraggins JM, Sarno EN, Schey KL, Pessolani MC. MALDI imaging reveals lipid changes in the skin of leprosy patients before and after multidrug therapy (MDT). J Mass Spectrom. 2015 Dec; 50(12): 1374-85. PMID: 26634971, DOI: 10.1002/jms.3708, ISSN: 1096-9888.
Gessel M, Spraggins JM, Voziyan P, Hudson BG, Caprioli RM. Decellularization of intact tissue enables MALDI imaging mass spectrometry analysis of the extracellular matrix. J Mass Spectrom. 2015 Nov; 50(11): 1288-93. PMID: 26505774, PMCID: PMC5320948, DOI: 10.1002/jms.3696, ISSN: 1096-9888.
Adler L, Boyer NP, Anderson DM, Spraggins JM, Schey KL, Hanneken A, Ablonczy Z, Crouch RK, Koutalos Y. Determination of N-retinylidene-N-retinylethanolamine (A2E) levels in central and peripheral areas of human retinal pigment epithelium. Photochem Photobiol Sci. 2015 Nov; 14(11): 1983-90. PMID: 26323192, PMCID: PMC4626405, DOI: 10.1039/c5pp00156k, ISSN: 1474-9092.
Wenke JL, Rose KL, Spraggins JM, Schey KL. MALDI Imaging Mass Spectrometry Spatially Maps Age-Related Deamidation and Truncation of Human Lens Aquaporin-0. Invest Ophthalmol Vis Sci. 2015 Nov; 56(12): 7398-405. PMID: 26574799, PMCID: PMC4675204, PII: 2471263, DOI: 10.1167/iovs.15-18117, ISSN: 1552-5783.
Marien E, Meister M, Muley T, Fieuws S, Bordel S, Derua R, Spraggins J, Van de Plas R, Dehairs J, Wouters J, Bagadi M, Dienemann H, Thomas M, Schnabel PA, Caprioli RM, Waelkens E, Swinnen JV. Non-small cell lung cancer is characterized by dramatic changes in phospholipid profiles. Int J Cancer [print-electronic]. 2015 Oct 10/1/2015; 137(7): 1539-48. PMID: 25784292, PMCID: PMC4503522, DOI: 10.1002/ijc.29517, ISSN: 1097-0215.
Wijetunge CD, Saeed I, Boughton BA, Spraggins JM, Caprioli RM, Bacic A, Roessner U, Halgamuge SK. EXIMS: an improved data analysis pipeline based on a new peak picking method for EXploring Imaging Mass Spectrometry data. Bioinformatics [print-electronic]. 2015 Oct 10/1/2015; 31(19): 3198-206. PMID: 26063840, PII: btv356, DOI: 10.1093/bioinformatics/btv356, ISSN: 1367-4811.
Spraggins JM, Rizzo DG, Moore JL, Rose KL, Hammer ND, Skaar EP, Caprioli RM. MALDI FTICR IMS of Intact Proteins: Using Mass Accuracy to Link Protein Images with Proteomics Data. J Am Soc Mass Spectrom [print-electronic]. 2015 Jun; 26(6): 974-85. PMID: 25904064, PMCID: PMC4442642, DOI: 10.1007/s13361-015-1147-5, ISSN: 1879-1123.
Anderson DM, Spraggins JM, Rose KL, Schey KL. High spatial resolution imaging mass spectrometry of human optic nerve lipids and proteins. J Am Soc Mass Spectrom [print-electronic]. 2015 Jun; 26(6): 940-7. PMID: 25893273, PMCID: PMC5650057, DOI: 10.1007/s13361-015-1143-9, ISSN: 1879-1123.
Van de Plas R, Yang J, Spraggins J, Caprioli RM. Image fusion of mass spectrometry and microscopy: a multimodality paradigm for molecular tissue mapping. Nat Methods [print-electronic]. 2015 Apr; 12(4): 366-72. PMID: 25707028, PMCID: PMC4382398, PII: nmeth.3296, DOI: 10.1038/nmeth.3296, ISSN: 1548-7105.
Manier ML, Spraggins JM, Reyzer ML, Norris JL, Caprioli RM. A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry. J Mass Spectrom. 2014 Aug; 49(8): 665-73. PMID: 25044893, PMCID: PMC4126081, DOI: 10.1002/jms.3411, ISSN: 1096-9888.
Anderson DM, Ablonczy Z, Koutalos Y, Spraggins J, Crouch RK, Caprioli RM, Schey KL. High resolution MALDI imaging mass spectrometry of retinal tissue lipids. J Am Soc Mass Spectrom [print-electronic]. 2014 Aug; 25(8): 1394-403. PMID: 24819461, PMCID: PMC4180438, DOI: 10.1007/s13361-014-0883-2, ISSN: 1879-1123.
Grove KJ, Voziyan PA, Spraggins JM, Wang S, Paueksakon P, Harris RC, Hudson BG, Caprioli RM. Diabetic nephropathy induces alterations in the glomerular and tubule lipid profiles. J Lipid Res [print-electronic]. 2014 Jul; 55(7): 1375-85. PMID: 24864273, PMCID: PMC4076088, PII: S0022-2275(20)35244-5, DOI: 10.1194/jlr.M049189, ISSN: 1539-7262.
Ablonczy Z, Smith N, Anderson DM, Grey AC, Spraggins J, Koutalos Y, Schey KL, Crouch RK. The utilization of fluorescence to identify the components of lipofuscin by imaging mass spectrometry. Proteomics [print-electronic]. 2014 Apr; 14(7-8): 936-44. PMID: 24453194, PMCID: PMC4017854, DOI: 10.1002/pmic.201300406, ISSN: 1615-9861.
Anderson DM, Mills D, Spraggins J, Lambert WS, Calkins DJ, Schey KL. High-resolution matrix-assisted laser desorption ionization-imaging mass spectrometry of lipids in rodent optic nerve tissue. Mol Vis [print-electronic]. 2013; 19: 581-92. PMID: 23559852, PMCID: PMC3611942, ISSN: 1090-0535.
Thiery G, Mernaugh RL, Yan H, Spraggins JM, Yang J, Parl FF, Caprioli RM. Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies. J Am Soc Mass Spectrom [print-electronic]. 2012 Oct; 23(10): 1689-96. PMID: 22869296, PMCID: PMC3525520, DOI: 10.1007/s13361-012-0423-x, ISSN: 1879-1123.
Angel PM, Spraggins JM, Baldwin HS, Caprioli R. Enhanced sensitivity for high spatial resolution lipid analysis by negative ion mode matrix assisted laser desorption ionization imaging mass spectrometry. Anal Chem [print-electronic]. 2012 Feb 2/7/2012; 84(3): 1557-64. PMID: 22243218, PMCID: PMC3277660, DOI: 10.1021/ac202383m, ISSN: 1520-6882.
Berry KA, Hankin JA, Barkley RM, Spraggins JM, Caprioli RM, Murphy RC. MALDI imaging of lipid biochemistry in tissues by mass spectrometry. Chem Rev [print-electronic]. 2011 Oct 10/12/2011; 111(10): 6491-512. PMID: 21942646, PMCID: PMC3199966, DOI: 10.1021/cr200280p, ISSN: 1520-6890.
Spraggins JM, Caprioli RM. High-speed MALDI-TOF imaging mass spectrometry: rapid ion image acquisition and considerations for next generation instrumentation. J Am Soc Mass Spectrom [print-electronic]. 2011 Jun; 22(6): 1022-31. PMID: 21953043, PMCID: PMC3514015, DOI: 10.1007/s13361-011-0121-0, ISSN: 1879-1123.
Postdoctoral Positions – Developing and Applying Multimodal Molecular Imaging Technologies
Our research group develops integrated molecular imaging technologies to elucidate the molecular basis of health and disease. Modern instrumentation and computing capabilities have enabled researchers to move beyond reductionist biology and, instead, probe how the components of biological entities (e.g. molecules, cells, and tissues) interact globally to reveal the underlying biology of disease. This systems biology approach has been accelerated by advancements in high-throughput ‘omics’ technologies, however, genetic and molecular information are only part of the story. The challenge lies in understanding how these parts interact and how perturbations to the system relate to disease. To address this challenge, we are advancing instrumental capabilities and developing the computational tools necessary to integrate and mine multimodal data sets that bring together imaging mass spectrometry, highly multiplexed immunofluorescence microscopy, and spatial transcriptomics.
The Spraggins laboratory is part of the Mass Spectrometry Research Center and Department of Cell & Developmental Biology at Vanderbilt University. We have multiple projects available including developing MALDI imaging instrumentation, integrated imaging mass spectrometry and spatial transcriptomics workflows, highly multiplexed immunofluorescence capabilities, spatial proteomics technologies, and computational data integration and mining tools. These projects involve exciting biomedical research applications including (1) constructing molecular atlases of human tissue as part of the Human Biomolecular Atlas Project (HuBMAP, https://hubmapconsortium.org) and understanding the molecular drivers of (2) host-pathogen interactions and (3) kidney disease.
Post-doctoral candidates joining the Mass Spectrometry Research Center will have access to state-of-the-art instrumentation, including 9T and 15T Bruker Fourier transform ion cyclotron mass spectrometers with MALDI and ESI sources, two Bruker timsTOF ion mobility/Q-TOF mass spectrometers with MALDI and ESI capabilities, a Bruker Rapiflex MALDI tissuetyper, and a Thermo Orbitrap Elite mass spectrometer with a Spectroglyph transmission geometry source capable of 1 micron imaging. We also have high-performance LC-MS/MS instrumentation including a Thermo Orbitrap Fusion Tribrid mass spectrometer, a Bruker Impact II Q-TOF with a nanoElute LC system, and an Agilent AssayMAP Bravo liquid handler. Multimodal and microscopy-based imaging instrumentation includes a GeoMX Digitial Spatial Profiler, a Zeiss Axio Observer Z1 inverted microscope integrated with an Akoya CODEX system, and a Zeiss Axio Scan Z1 brightfield and fluorescence slide scanner. The Mass Spectrometry Research Center also hosts a collection of commercial and state-of-the-art custom software for the analysis of imaging, multi-omics, and microscopy data.
Please contact Jeff Spraggins at jeff.spraggins@vanderbilt.edu if you are interested in learning more about these opportunities.
Vanderbilt University is committed to the acceptance of a diverse group of trainees that is populated with people of all races, ethnicities, genders, gender identities, sexual orientation, disabilities, all places of geographic origin and the full spectrum of socio-economic status.