Faculty Appointments
Senior Assistant Dean, Biomedical Research, Education, and Training (BRET) Professor of Pathology, Microbiology and Immunology
Professor of Urology
Education
Ph.D., MICRO AND MOLECULAR GENETICS, University of Texas Health Science Center, Houston, TexasB.S., Clarion University of Pennsylvania, Clarion, Pennsylvania
Office Address
Vanderbilt University School of Medicine
Dept of Pathology, Microbiology and Immunology-5th Fl
MCN A-5301
Nashville, TN 37232-2363
Dept of Pathology, Microbiology and Immunology-5th Fl
MCN A-5301
Nashville, TN 37232-2363
Research Description
Urinary tract infections (UTIs) are among the most frequent bacterial infections, are highly prevalent among women and have a high degree of recurrence. Currently, antibiotics are the primary treatment option for UTI, however they oftentimes fail to eliminate infection, they perturb the host microbiota and select for increased antibiotic resistance. This means that there is a pressing need for the development of alternative strategies for preventing and/or treating UTIs. Uropathogenic Escherichia coli (UPEC), which causes 85% of all UTIs, has evolved a remarkable mechanism to evade host immune defenses and establish infection, by forming biofilm-like intracellular bacterial communities (IBC) inside bladder cells, in addition to forming extracellular biofilms on host cell surfaces and on catheter implants. My lab utilizes molecular, and imaging techniques coupled with robust murine models of UTI to improve the understanding of disease mechanisms in the urinary tract and develop methods for treatment, and prevention of UTI. Towards this goal, I have developed over the years a massive library of clean deletion mutants in uropathogenic Escherichia coli that encompasses metabolism, motility and chemo-sensing regulatory genes, among others. My research group has additionally developed pipelines for multi-omics approaches that dissect the genomic diversity of uropathogens, probe metabolic distinctions among symptom-causing and asymptomatic colonizers of the urinary
tract and investigate the role of the urinary and vaginal microbiomes in influencing infection dynamics, or the response of pathogens to antibiotics. We are one of the top 3 laboratories in urinary tract infection research focusing specifically on uropathogen behavior and interaction with the host. Our most prominent discoveries focusing on uropathogenic Escherichia coli (UPEC) include: 1) We delineated regulatory mechanisms of biofilm formation, a key pathogen property that impairs antibiotic effectiveness during infection. 2) We have uncovered bacterial signaling networks that are required in specific niches during infection and chronic persistence (Brannon et al., 2024 Microbiology Spectrum). 3) We discovered a new intracellular pathway for UPEC in the vagina (Brannon et al., 2020 Nature Communications). 4) We identified that UPEC respires aerobically in the bladder and dissected metabolic requirements for UPEC intracellular expansion (Beebout et al., 2022, Nature Microbiology). We welcome creative individuals to our group who are excited about mechanistic, or translational research.
tract and investigate the role of the urinary and vaginal microbiomes in influencing infection dynamics, or the response of pathogens to antibiotics. We are one of the top 3 laboratories in urinary tract infection research focusing specifically on uropathogen behavior and interaction with the host. Our most prominent discoveries focusing on uropathogenic Escherichia coli (UPEC) include: 1) We delineated regulatory mechanisms of biofilm formation, a key pathogen property that impairs antibiotic effectiveness during infection. 2) We have uncovered bacterial signaling networks that are required in specific niches during infection and chronic persistence (Brannon et al., 2024 Microbiology Spectrum). 3) We discovered a new intracellular pathway for UPEC in the vagina (Brannon et al., 2020 Nature Communications). 4) We identified that UPEC respires aerobically in the bladder and dissected metabolic requirements for UPEC intracellular expansion (Beebout et al., 2022, Nature Microbiology). We welcome creative individuals to our group who are excited about mechanistic, or translational research.
Research Keywords
Antibiotic resistance, bacteriology, biofilms, heterogeneity, microbiology, pathogenesis, signaling mechanisms, transcriptional regulation, UPEC, UTIs.
Publications
Reasoner SA, Frainey BT, Hale OF, Borden A, Graham MK, Turner E, Brenes LR, Soderstrom CBW, Green H, Schmitz JE, Laub MT, Kelly MS, Clayton DB, Hadjifrangiskou M. Unmasking pathogen traits for chronic colonization in neurogenic bladder. Cell Rep [print-electronic]. 2026 Apr 4/22/2026; 45(5): 117302. PMID: 42024499, PII: S2211-1247(26)00380-3, DOI: 10.1016/j.celrep.2026.117302, ISSN: 2211-1247.
Reasoner S, Francis J, Gidney M, Amponsah A, Frainey B, Schrepf A, Kelly AG, Ryden A, Crofford L, Dmochowski R, Hadjifrangiskou M, McKernan L. Urobiome Analysis in Interstitial Cystitis/Bladder Pain Syndrome Reveals Nuanced Differences Associated with Localized Pain. BioRxiv. 2026 Jan 1/2/2026; PMID: 41502943, PMCID: PMC12772995, PII: 2026.01.02.697114, DOI: 10.64898/2026.01.02.697114, ISSN: 2692-8205.
Lee JW, Wang L, Comer SL, Colin R, Gidney MM, Laganenka L, Hardt WD, Hadjifrangiskou M, Sourjik V. Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli. EMBO J [print-electronic]. 2025 Nov; 44(22): 6675-703. PMID: 41083908, PMCID: PMC12623472, PII: 10.1038/s44318-025-00595-x, DOI: 10.1038/s44318-025-00595-x, ISSN: 1460-2075.
Reasoner SA, Francis J, Hadjifrangiskou M. The urinary microbiome: the next frontier of bacterial ecology. J Bacteriol [print-electronic]. 2025 Aug 8/21/2025; 207(8): e0010525. PMID: 40704791, PMCID: PMC12369349, DOI: 10.1128/jb.00105-25, ISSN: 1098-5530.
Green HD, Van Horn GT, Williams T, Eberly A, Morales GH, Mann R, Hauter IM, Hadjifrangiskou M, Schmitz JE. Intra-strain colony biofilm heterogeneity in uropathogenic Escherichia coli and the effect of the NlpI lipoprotein. Biofilm. 2024 Dec; 8: 100214. PMID: 39184815, PMCID: PMC11344014, PII: S2590-2075(24)00039-X, DOI: 10.1016/j.bioflm.2024.100214, ISSN: 2590-2075.
Brannon JR, Reasoner SA, Bermudez TA, Comer SL, Wiebe MA, Dunigan TL, Beebout CJ, Ross T, Bamidele A, Hadjifrangiskou M. Mapping niche-specific two-component system requirements in uropathogenic Escherichia coli. Microbiol Spectr [print-electronic]. 2024 Apr 4/2/2024; 12(4): e0223623. PMID: 38385738, PMCID: PMC10986536, DOI: 10.1128/spectrum.02236-23, ISSN: 2165-0497.
Reasoner SA, Bernard R, Waalkes A, Penewit K, Lewis J, Sokolow AG, Brown RF, Edwards KM, Salipante SJ, Hadjifrangiskou M, Nicholson MR. Longitudinal profiling of the intestinal microbiome in children with cystic fibrosis treated with elexacaftor-tezacaftor-ivacaftor. MBio [print-electronic]. 2024 Feb 2/14/2024; 15(2): e0193523. PMID: 38275294, PMCID: PMC10865789, DOI: 10.1128/mbio.01935-23, ISSN: 2150-7511.
Bermudez TA, Brannon JR, Dudipala N, Reasoner S, Morales G, Wiebe M, Cecala M, DaCosta M, Beebout C, Amir O, Hadjifrangiskou M. Raising the alarm: fosfomycin resistance associated with non-susceptible inner colonies imparts no fitness cost to the primary bacterial uropathogen. Antimicrob Agents Chemother [print-electronic]. 2024 Jan 1/10/2024; 68(1): e0080323. PMID: 38078906, PMCID: PMC10777853, DOI: 10.1128/aac.00803-23, ISSN: 1098-6596.
Reasoner SA, Flores V, Van Horn G, Morales G, Peard LM, Abelson B, Manuel C, Lee J, Baker B, Williams T, Schmitz JE, Clayton DB, Hadjifrangiskou M. Survey of the infant male urobiome and genomic analysis of Actinotignum spp. NPJ Biofilms Microbiomes. 2023 Dec 12/1/2023; 9(1): 91. PMID: 38040700, PMCID: PMC10692110, PII: 10.1038/s41522-023-00457-6, DOI: 10.1038/s41522-023-00457-6, ISSN: 2055-5008.
Reasoner SA, Bernard R, Waalkes A, Penewit K, Lewis J, Sokolow AG, Brown RF, Edwards KM, Salipante SJ, Hadjifrangiskou M, Nicholson MR. Longitudinal Profiling of the Intestinal Microbiome in Children with Cystic Fibrosis Treated with Elexacaftor-Tezacaftor-Ivacaftor. MedRxiv. 2023 Nov 11/21/2023; PMID: 37645804, PMCID: PMC10462202, PII: 2023.08.11.23293949, DOI: 10.1101/2023.08.11.23293949.
Hurst MN, Beebout CJ, Hollingsworth A, Guckes KR, Purcell A, Bermudez TA, Williams D, Reasoner SA, Trent MS, Hadjifrangiskou M. The QseB response regulator imparts tolerance to positively charged antibiotics by controlling metabolism and minor changes to LPS. MSphere [print-electronic]. 2023 Oct 10/24/2023; 8(5): e0005923. PMID: 37676915, PMCID: PMC10597456, DOI: 10.1128/msphere.00059-23, ISSN: 2379-5042.
Hadjifrangiskou M. Sampling the rainbow. Nat Chem Biol. 2023 Aug; 19(8): 928-9. PMID: 37055615, PII: 10.1038/s41589-023-01305-6, DOI: 10.1038/s41589-023-01305-6, ISSN: 1552-4469.
Morales G, Abelson B, Reasoner S, Miller J, Earl AM, Hadjifrangiskou M, Schmitz J. The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria. Microbiol Spectr [print-electronic]. 2023 Jun 6/15/2023; 11(3): e0471022. PMID: 37195213, PMCID: PMC10269530, DOI: 10.1128/spectrum.04710-22, ISSN: 2165-0497.
Hadjifrangiskou M, Liu J, Ottemann KM, Roujeinikova A. A peek inside the 2023 Bacterial Locomotion and Signal Transduction (BLAST) conference. Trends Microbiol [print-electronic]. 2023 Jun; 31(6): 539-43. PMID: 37127442, PII: S0966-842X(23)00096-3, DOI: 10.1016/j.tim.2023.03.016, ISSN: 1878-4380.
Pilewski KA, Wall S, Richardson SI, Manamela NP, Clark K, Hermanus T, Binshtein E, Venkat R, Sautto GA, Kramer KJ, Shiakolas AR, Setliff I, Salas J, Mapengo RE, Suryadevara N, Brannon JR, Beebout CJ, Parks R, Raju N, Frumento N, Walker LM, Fechter EF, Qin JS, Murji AA, Janowska K, Thakur B, Lindenberger J, May AJ, Huang X, Sammour S, Acharya P, Carnahan RH, Ross TM, Haynes BF, Hadjifrangiskou M, Crowe JE, Bailey JR, Kalams S, Morris L, Georgiev IS. Functional HIV-1/HCV cross-reactive antibodies isolated from a chronically co-infected donor. Cell Rep [print-electronic]. 2023 Feb 2/28/2023; 42(2): 112044. PMID: 36708513, PMCID: PMC10372200, PII: S2211-1247(23)00055-4, DOI: 10.1016/j.celrep.2023.112044, ISSN: 2211-1247.
Wiebe MA, Brannon JR, Steiner BD, Bamidele A, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, Hadjifrangiskou M. Serine Deamination Is a New Acid Tolerance Mechanism Observed in Uropathogenic Escherichia coli. MBio [print-electronic]. 2022 Dec 12/20/2022; 13(6): e0296322. PMID: 36468870, PMCID: PMC9765748, DOI: 10.1128/mbio.02963-22, ISSN: 2150-7511.
Sultana S, Crompton ME, Meurer K, Jankiewicz O, Morales GH, Johnson C, Horbach E, Hoffmann KP, Kr P, Shah R, Anderson GM, Mortimer NT, Schmitz JE, Hadjifrangiskou M, Foti A, Dahl JU. Redox-Mediated Inactivation of the Transcriptional Repressor RcrR is Responsible for Uropathogenic Escherichia coli's Increased Resistance to Reactive Chlorine Species. MBio [print-electronic]. 2022 Oct 10/26/2022; 13(5): e0192622. PMID: 36073817, PMCID: PMC9600549, DOI: 10.1128/mbio.01926-22, ISSN: 2150-7511.
Beebout CJ, Robertson GL, Reinfeld BI, Blee AM, Morales GH, Brannon JR, Chazin WJ, Rathmell WK, Rathmell JC, Gama V, Hadjifrangiskou M. Uropathogenic Escherichia coli subverts mitochondrial metabolism to enable intracellular bacterial pathogenesis in urinary tract infection. Nat Microbiol [print-electronic]. 2022 Sep; 7(9): 1348-60. PMID: 35995841, PMCID: PMC9756876, PII: 10.1038/s41564-022-01205-w, DOI: 10.1038/s41564-022-01205-w, ISSN: 2058-5276.
Fleming BA, Blango MG, Rousek AA, Kincannon WM, Tran A, Lewis AJ, Russell CW, Zhou Q, Baird LM, Barber AE, Brannon JR, Beebout CJ, Bandarian V, Hadjifrangiskou M, Howard MT, Mulvey MA. A tRNA modifying enzyme as a tunable regulatory nexus for bacterial stress responses and virulence. Nucleic Acids Res. 2022 Jul 7/22/2022; 50(13): 7570-90. PMID: 35212379, PMCID: PMC9303304, PII: 6536892, DOI: 10.1093/nar/gkac116, ISSN: 1362-4962.
Steenwyk JL, Buida TJ, Gonçalves C, Goltz DC, Morales G, Mead ME, LaBella AL, Chavez CM, Schmitz JE, Hadjifrangiskou M, Li Y, Rokas A. BioKIT: a versatile toolkit for processing and analyzing diverse types of sequence data. Genetics. 2022 Jul 7/4/2022; 221(3): PMID: 35536198, PMCID: PMC9252278, PII: 6583183, DOI: 10.1093/genetics/iyac079, ISSN: 1943-2631.
Reasoner SA, Enriquez KT, Abelson B, Scaglione S, Schneier B, O'Connor MG, Van Horn G, Hadjifrangiskou M. Urinary tract infections in cystic fibrosis patients. J Cyst Fibros [print-electronic]. 2022 Jan; 21(1): e1-e4-e4. PMID: 34330649, PMCID: PMC9627942, PII: S1569-1993(21)01303-5, DOI: 10.1016/j.jcf.2021.07.005, ISSN: 1873-5010.
Beebout CJ, Sominsky LA, Eberly AR, Van Horn GT, Hadjifrangiskou M. Cytochrome bd promotes Escherichia coli biofilm antibiotic tolerance by regulating accumulation of noxious chemicals. NPJ Biofilms Microbiomes. 2021 Apr 4/16/2021; 7(1): 35. PMID: 33863914, PMCID: PMC8052454, PII: 10.1038/s41522-021-00210-x, DOI: 10.1038/s41522-021-00210-x, ISSN: 2055-5008.
Eberly AR, Beebout CJ, Tong CMC, Van Horn GT, Green HD, Fitzgerald MJ, De S, Apple EK, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, Clayton DB, Stratton CW, Schmitz JE, Hadjifrangiskou M. Data highlighting phenotypic diversity of urine-associated Escherichia coli isolates. Data Brief. 2020 Aug; 31: 105811. PMID: 32566710, PMCID: PMC7298415, PII: S2352-3409(20)30705-8, DOI: 10.1016/j.dib.2020.105811, ISSN: 2352-3409.
Brannon JR, Dunigan TL, Beebout CJ, Ross T, Wiebe MA, Reynolds WS, Hadjifrangiskou M. Invasion of vaginal epithelial cells by uropathogenic Escherichia coli. Nat Commun. 2020 Jun 6/4/2020; 11(1): 2803. PMID: 32499566, PMCID: PMC7272400, PII: 10.1038/s41467-020-16627-5, DOI: 10.1038/s41467-020-16627-5, ISSN: 2041-1723.
Eberly AR, Beebout CJ, Carmen Tong CM, Van Horn GT, Green HD, Fitzgerald MJ, De S, Apple EK, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, Clayton DB, Stratton CW, Schmitz JE, Hadjifrangiskou M. Defining a Molecular Signature for Uropathogenic versus Urocolonizing Escherichia coli: The Status of the Field and New Clinical Opportunities. J Mol Biol [print-electronic]. 2020 Feb 2/14/2020; 432(4): 786-804. PMID: 31794727, PMCID: PMC7293133, PII: S0022-2836(19)30672-2, DOI: 10.1016/j.jmb.2019.11.008, ISSN: 1089-8638.
Beebout CJ, Eberly AR, Werby SH, Reasoner SA, Brannon JR, De S, Fitzgerald MJ, Huggins MM, Clayton DB, Cegelski L, Hadjifrangiskou M. Respiratory Heterogeneity Shapes Biofilm Formation and Host Colonization in Uropathogenic Escherichia coli. MBio. 2019 Apr 4/2/2019; 10(2): PMID: 30940709, PMCID: PMC6445943, PII: mBio.02400-18, DOI: 10.1128/mBio.02400-18, ISSN: 2150-7511.
Magana M, Sereti C, Ioannidis A, Mitchell CA, Ball AR, Magiorkinis E, Chatzipanagiotou S, Hamblin MR, Hadjifrangiskou M, Tegos GP. Options and Limitations in Clinical Investigation of Bacterial Biofilms. Clin Microbiol Rev [electronic-print]. 2018 Jul; 31(3): PMID: 29618576, PMCID: PMC6056845, PII: 31/3/e00084-16, DOI: 10.1128/CMR.00084-16, ISSN: 1098-6618.
Steiner BD, Eberly AR, Hurst MN, Zhang EW, Green HD, Behr S, Jung K, Hadjifrangiskou M. Evidence of Cross-Regulation in Two Closely Related Pyruvate-Sensing Systems in Uropathogenic Escherichia coli. J Membr Biol [print-electronic]. 2018 Feb; 251(1): 65-74. PMID: 29374286, PMCID: PMC5834395, PII: 10.1007/s00232-018-0014-2, DOI: 10.1007/s00232-018-0014-2, ISSN: 1432-1424.
Dale JL, Raynor MJ, Ty MC, Hadjifrangiskou M, Koehler TM. A Dual Role for the Bacillus anthracis Master Virulence Regulator AtxA: Control of Sporulation and Anthrax Toxin Production. Front Microbiol. 2018; 9: 482. PMID: 29599764, PMCID: PMC5862856, DOI: 10.3389/fmicb.2018.00482, ISSN: 1664-302X.
Eberly AR, Floyd KA, Beebout CJ, Colling SJ, Fitzgerald MJ, Stratton CW, Schmitz JE, Hadjifrangiskou M. Biofilm Formation by Uropathogenic Escherichia coli Is Favored under Oxygen Conditions That Mimic the Bladder Environment. Int J Mol Sci. 2017 Sep 9/30/2017; 18(10): PMID: 28973965, PMCID: PMC5666759, PII: ijms18102077, DOI: 10.3390/ijms18102077, ISSN: 1422-0067.
Abana CM, Brannon JR, Ebbott RA, Dunigan TL, Guckes KR, Fuseini H, Powers J, Rogers BR, Hadjifrangiskou M. Characterization of blue light irradiation effects on pathogenic and nonpathogenic Escherichia coli. Microbiologyopen [print-electronic]. 2017 Aug; 6(4): PMID: 28332311, PMCID: PMC5552948, DOI: 10.1002/mbo3.466, ISSN: 2045-8827.
Behr S, Kristoficova I, Witting M, Breland EJ, Eberly AR, Sachs C, Schmitt-Kopplin P, Hadjifrangiskou M, Jung K. Identification of a High-Affinity Pyruvate Receptor in Escherichia coli. Sci Rep. 2017 May 5/3/2017; 7(1): 1388. PMID: 28469239, PMCID: PMC5431176, PII: 10.1038/s41598-017-01410-2, DOI: 10.1038/s41598-017-01410-2, ISSN: 2045-2322.
Breland EJ, Zhang EW, Bermudez T, Martinez CR, Hadjifrangiskou M. The histidine residue of QseC is required for canonical signaling between QseB and PmrB in uropathogenic Escherichia coli. J. Bacteriol [print-electronic]. 2017 Apr 4/10/2017; PMID: 28396353, PII: JB.00060-17, DOI: 10.1128/JB.00060-17, ISSN: 1098-5530.
Guckes KR, Breland EJ, Zhang EW, Hanks SC, Gill NK, Algood HM, Schmitz JE, Stratton CW, Hadjifrangiskou M. Signaling by two-component system noncognate partners promotes intrinsic tolerance to polymyxin B in uropathogenic Escherichia coli. Sci Signal. 2017 Jan 1/10/2017; 10(461): PMID: 28074004, PII: 10/461/eaag1775, DOI: 10.1126/scisignal.aag1775, ISSN: 1937-9145.
Breland EJ, Eberly AR, Hadjifrangiskou M. An Overview of Two-Component Signal Transduction Systems Implicated in Extra-Intestinal Pathogenic E. coli Infections. Front Cell Infect Microbiol. 2017; 7: 162. PMID: 28536675, PMCID: PMC5422438, DOI: 10.3389/fcimb.2017.00162, ISSN: 2235-2988.
Shaffer CL, Zhang EW, Dudley AG, Dixon BR, Guckes KR, Breland EJ, Floyd KA, Casella DP, Algood HM, Clayton DB, Hadjifrangiskou M. Purine Biosynthesis Metabolically Constrains Intracellular Survival of Uropathogenic Escherichia coli. Infect. Immun [electronic-print]. 2017 Jan; 85(1): PMID: 27795353, PMCID: PMC5203662, PII: IAI.00471-16, DOI: 10.1128/IAI.00471-16, ISSN: 1098-5522.
Varga MG, Shaffer CL, Sierra JC, Suarez G, Piazuelo MB, Whitaker ME, Romero-Gallo J, Krishna US, Delgado A, Gomez MA, Good JA, Almqvist F, Skaar EP, Correa P, Wilson KT, Hadjifrangiskou M, Peek RM. Pathogenic Helicobacter pylori strains translocate DNA and activate TLR9 via the cancer-associated cag type IV secretion system. Oncogene [print-electronic]. 2016 Dec 12/1/2016; 35(48): 6262-9. PMID: 27157617, PMCID: PMC5102820, PII: onc2016158, DOI: 10.1038/onc.2016.158, ISSN: 1476-5594.
Floyd KA, Mitchell CA, Eberly AR, Colling SJ, Zhang EW, DePas W, Chapman MR, Conover M, Rogers BR, Hultgren SJ, Hadjifrangiskou M. The UbiI (VisC) Aerobic Ubiquinone Synthase Is Required for Expression of Type 1 Pili, Biofilm Formation, and Pathogenesis in Uropathogenic Escherichia coli. J. Bacteriol [electronic-print]. 2016 Oct 10/1/2016; 198(19): 2662-72. PMID: 27161114, PMCID: PMC5019064, PII: JB.00030-16, DOI: 10.1128/JB.00030-16, ISSN: 1098-5530.
Shaffer CL, Good JA, Kumar S, Krishnan KS, Gaddy JA, Loh JT, Chappell J, Almqvist F, Cover TL, Hadjifrangiskou M. Peptidomimetic Small Molecules Disrupt Type IV Secretion System Activity in Diverse Bacterial Pathogens. MBio. 2016 Apr 4/26/2016; 7(2): e00221-16. PMID: 27118587, PMCID: PMC4850256, PII: mBio.00221-16, DOI: 10.1128/mBio.00221-16, ISSN: 2150-7511.
Conover MS, Hadjifrangiskou M, Palermo JJ, Hibbing ME, Dodson KW, Hultgren SJ. Metabolic Requirements of Escherichia coli in Intracellular Bacterial Communities during Urinary Tract Infection Pathogenesis. MBio. 2016 Apr 4/12/2016; 7(2): e00104-16-e116. PMID: 27073089, PMCID: PMC4959519, PII: mBio.00104-16, DOI: 10.1128/mBio.00104-16, ISSN: 2150-7511.
Brannon JR, Hadjifrangiskou M. The arsenal of pathogens and antivirulence therapeutic strategies for disarming them. Drug Des Devel Ther. 2016; 10: 1795-806. PMID: 27313446, PMCID: PMC4890686, PII: dddt-10-1795, DOI: 10.2147/DDDT.S98939, ISSN: 1177-8881.
Floyd KA, Meyer AE, Nelson G, Hadjifrangiskou M. The yin-yang driving urinary tract infection and how proteomics can enhance research, diagnostics, and treatment. Proteomics Clin Appl [print-electronic]. 2015 Dec; 9(11-12): 990-1002. PMID: 26255866, DOI: 10.1002/prca.201500018, ISSN: 1862-8354.
Floyd KA, Moore JL, Eberly AR, Good JA, Shaffer CL, Zaver H, Almqvist F, Skaar EP, Caprioli RM, Hadjifrangiskou M. Adhesive fiber stratification in uropathogenic Escherichia coli biofilms unveils oxygen-mediated control of type 1 pili. PLoS Pathog. 2015 Mar; 11(3): e1004697. PMID: 25738819, PMCID: PMC4349694, PII: PPATHOGENS-D-14-02041, DOI: 10.1371/journal.ppat.1004697, ISSN: 1553-7374.
Nagamatsu K, Hannan TJ, Guest RL, Kostakioti M, Hadjifrangiskou M, Binkley J, Dodson K, Raivio TL, Hultgren SJ. Dysregulation of Escherichia coli a-hemolysin expression alters the course of acute and persistent urinary tract infection. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Feb 2/24/2015; 112(8): E871-80. PMID: 25675528, PMCID: PMC4345586, PII: 1500374112, DOI: 10.1073/pnas.1500374112, ISSN: 1091-6490.
Greene SE, Pinkner JS, Chorell E, Dodson KW, Shaffer CL, Conover MS, Livny J, Hadjifrangiskou M, Almqvist F, Hultgren SJ. Pilicide ec240 disrupts virulence circuits in uropathogenic Escherichia coli. MBio. 2014; 5(6): e02038. PMID: 25352623, PMCID: PMC4217179, PII: mBio.02038-14, DOI: 10.1128/mBio.02038-14, ISSN: 2150-7511.
Guckes KR, Kostakioti M, Breland EJ, Gu AP, Shaffer CL, Martinez CR, Hultgren SJ, Hadjifrangiskou M. Strong cross-system interactions drive the activation of the QseB response regulator in the absence of its cognate sensor. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Oct 10/8/2013; 110(41): 16592-7. PMID: 24062463, PMCID: PMC3799328, PII: 1315320110, DOI: 10.1073/pnas.1315320110, ISSN: 1091-6490.
Kostakioti M, Hadjifrangiskou M, Hultgren SJ. Bacterial biofilms: development, dispersal, and therapeutic strategies in the dawn of the postantibiotic era. Cold Spring Harb Perspect Med. 2013 Apr; 3(4): a010306. PMID: 23545571, PMCID: PMC3683961, PII: 3/4/a010306, DOI: 10.1101/cshperspect.a010306, ISSN: 2157-1422.
Hung C, Zhou Y, Pinkner JS, Dodson KW, Crowley JR, Heuser J, Chapman MR, Hadjifrangiskou M, Henderson JP, Hultgren SJ. Escherichia coli biofilms have an organized and complex extracellular matrix structure. MBio. 2013; 4(5): e00645-13. PMID: 24023384, PMCID: PMC3774191, PII: mBio.00645-13, DOI: 10.1128/mBio.00645-13, ISSN: 2150-7511.
Kostakioti M, Hultgren SJ, Hadjifrangiskou M. Molecular blueprint of uropathogenic Escherichia coli virulence provides clues toward the development of anti-virulence therapeutics. Virulence [print-electronic]. 2012 Nov 11/15/2012; 3(7): 592-4. PMID: 23154288, PMCID: PMC3545937, PII: 22364, DOI: 10.4161/viru.22364, ISSN: 2150-5608.
Hadjifrangiskou M, Gu AP, Pinkner JS, Kostakioti M, Zhang EW, Greene SE, Hultgren SJ. Transposon mutagenesis identifies uropathogenic Escherichia coli biofilm factors. J. Bacteriol [print-electronic]. 2012 Nov; 194(22): 6195-205. PMID: 22984258, PMCID: PMC3486386, PII: JB.01012-12, DOI: 10.1128/JB.01012-12, ISSN: 1098-5530.
Kostakioti M, Hadjifrangiskou M, Cusumano CK, Hannan TJ, Janetka JW, Hultgren SJ. Distinguishing the contribution of type 1 pili from that of other QseB-misregulated factors when QseC is absent during urinary tract infection. Infect. Immun [print-electronic]. 2012 Aug; 80(8): 2826-34. PMID: 22665375, PMCID: PMC3434567, PII: IAI.00283-12, DOI: 10.1128/IAI.00283-12, ISSN: 1098-5522.
Hadjifrangiskou M, Hultgren SJ. What does it take to stick around? Molecular insights into biofilm formation by uropathogenic Escherichia coli. Virulence [print-electronic]. 2012 May 5/1/2012; 3(3): 231-3. PMID: 22546905, PMCID: PMC3442832, PII: 19763, DOI: 10.4161/viru.19763, ISSN: 2150-5608.
Hadjifrangiskou M, Kostakioti M, Hultgren SJ. Antitoxins: therapy for stressed bacteria. Nature chemical biology. 2011 Jun; 7(6) Sect. 345,7.
Hadjifrangiskou M, Kostakioti M, Chen SL, Henderson JP, Greene SE, Hultgren SJ. A central metabolic circuit controlled by QseC in pathogenic Escherichia coli. Mol. Microbiol [print-electronic]. 2011 Jun; 80(6): 1516-29. PMID: 21542868, PMCID: PMC3643513, DOI: 10.1111/j.1365-2958.2011.07660.x, ISSN: 1365-2958.
Kostakioti M, Hadjifrangiskou M, Pinkner JS, Hultgren SJ. QseC-mediated dephosphorylation of QseB is required for expression of genes associated with virulence in uropathogenic Escherichia coli. Mol. Microbiol [print-electronic]. 2009 Sep; 73(6): 1020-31. PMID: 19703104, PMCID: PMC2963169, PII: MMI6826, DOI: 10.1111/j.1365-2958.2009.06826.x, ISSN: 1365-2958.
Hadjifrangiskou M, Koehler TM. Intrinsic curvature associated with the coordinately regulated anthrax toxin gene promoters. Microbiology (Reading, Engl.). 2008 Aug; 154(Pt 8): 2501-12. PMID: 18667583, PMCID: PMC4225132, PII: 154/8/2501, DOI: 10.1099/mic.0.2007/016162-0, ISSN: 1350-0872.
Hadjifrangiskou M, Chen Y, Koehler TM. The alternative sigma factor sigmaH is required for toxin gene expression by Bacillus anthracis. J. Bacteriol [print-electronic]. 2007 Mar; 189(5): 1874-83. PMID: 17189374, PMCID: PMC1855707, PII: JB.01333-06, DOI: 10.1128/JB.01333-06, ISSN: 0021-9193.
Reasoner S, Francis J, Gidney M, Amponsah A, Frainey B, Schrepf A, Kelly AG, Ryden A, Crofford L, Dmochowski R, Hadjifrangiskou M, McKernan L. Urobiome Analysis in Interstitial Cystitis/Bladder Pain Syndrome Reveals Nuanced Differences Associated with Localized Pain. BioRxiv. 2026 Jan 1/2/2026; PMID: 41502943, PMCID: PMC12772995, PII: 2026.01.02.697114, DOI: 10.64898/2026.01.02.697114, ISSN: 2692-8205.
Lee JW, Wang L, Comer SL, Colin R, Gidney MM, Laganenka L, Hardt WD, Hadjifrangiskou M, Sourjik V. Control of flagellar gene expression by a chemotaxis receptor-like regulator in pathogenic Escherichia coli. EMBO J [print-electronic]. 2025 Nov; 44(22): 6675-703. PMID: 41083908, PMCID: PMC12623472, PII: 10.1038/s44318-025-00595-x, DOI: 10.1038/s44318-025-00595-x, ISSN: 1460-2075.
Reasoner SA, Francis J, Hadjifrangiskou M. The urinary microbiome: the next frontier of bacterial ecology. J Bacteriol [print-electronic]. 2025 Aug 8/21/2025; 207(8): e0010525. PMID: 40704791, PMCID: PMC12369349, DOI: 10.1128/jb.00105-25, ISSN: 1098-5530.
Green HD, Van Horn GT, Williams T, Eberly A, Morales GH, Mann R, Hauter IM, Hadjifrangiskou M, Schmitz JE. Intra-strain colony biofilm heterogeneity in uropathogenic Escherichia coli and the effect of the NlpI lipoprotein. Biofilm. 2024 Dec; 8: 100214. PMID: 39184815, PMCID: PMC11344014, PII: S2590-2075(24)00039-X, DOI: 10.1016/j.bioflm.2024.100214, ISSN: 2590-2075.
Brannon JR, Reasoner SA, Bermudez TA, Comer SL, Wiebe MA, Dunigan TL, Beebout CJ, Ross T, Bamidele A, Hadjifrangiskou M. Mapping niche-specific two-component system requirements in uropathogenic Escherichia coli. Microbiol Spectr [print-electronic]. 2024 Apr 4/2/2024; 12(4): e0223623. PMID: 38385738, PMCID: PMC10986536, DOI: 10.1128/spectrum.02236-23, ISSN: 2165-0497.
Reasoner SA, Bernard R, Waalkes A, Penewit K, Lewis J, Sokolow AG, Brown RF, Edwards KM, Salipante SJ, Hadjifrangiskou M, Nicholson MR. Longitudinal profiling of the intestinal microbiome in children with cystic fibrosis treated with elexacaftor-tezacaftor-ivacaftor. MBio [print-electronic]. 2024 Feb 2/14/2024; 15(2): e0193523. PMID: 38275294, PMCID: PMC10865789, DOI: 10.1128/mbio.01935-23, ISSN: 2150-7511.
Bermudez TA, Brannon JR, Dudipala N, Reasoner S, Morales G, Wiebe M, Cecala M, DaCosta M, Beebout C, Amir O, Hadjifrangiskou M. Raising the alarm: fosfomycin resistance associated with non-susceptible inner colonies imparts no fitness cost to the primary bacterial uropathogen. Antimicrob Agents Chemother [print-electronic]. 2024 Jan 1/10/2024; 68(1): e0080323. PMID: 38078906, PMCID: PMC10777853, DOI: 10.1128/aac.00803-23, ISSN: 1098-6596.
Reasoner SA, Flores V, Van Horn G, Morales G, Peard LM, Abelson B, Manuel C, Lee J, Baker B, Williams T, Schmitz JE, Clayton DB, Hadjifrangiskou M. Survey of the infant male urobiome and genomic analysis of Actinotignum spp. NPJ Biofilms Microbiomes. 2023 Dec 12/1/2023; 9(1): 91. PMID: 38040700, PMCID: PMC10692110, PII: 10.1038/s41522-023-00457-6, DOI: 10.1038/s41522-023-00457-6, ISSN: 2055-5008.
Reasoner SA, Bernard R, Waalkes A, Penewit K, Lewis J, Sokolow AG, Brown RF, Edwards KM, Salipante SJ, Hadjifrangiskou M, Nicholson MR. Longitudinal Profiling of the Intestinal Microbiome in Children with Cystic Fibrosis Treated with Elexacaftor-Tezacaftor-Ivacaftor. MedRxiv. 2023 Nov 11/21/2023; PMID: 37645804, PMCID: PMC10462202, PII: 2023.08.11.23293949, DOI: 10.1101/2023.08.11.23293949.
Hurst MN, Beebout CJ, Hollingsworth A, Guckes KR, Purcell A, Bermudez TA, Williams D, Reasoner SA, Trent MS, Hadjifrangiskou M. The QseB response regulator imparts tolerance to positively charged antibiotics by controlling metabolism and minor changes to LPS. MSphere [print-electronic]. 2023 Oct 10/24/2023; 8(5): e0005923. PMID: 37676915, PMCID: PMC10597456, DOI: 10.1128/msphere.00059-23, ISSN: 2379-5042.
Hadjifrangiskou M. Sampling the rainbow. Nat Chem Biol. 2023 Aug; 19(8): 928-9. PMID: 37055615, PII: 10.1038/s41589-023-01305-6, DOI: 10.1038/s41589-023-01305-6, ISSN: 1552-4469.
Morales G, Abelson B, Reasoner S, Miller J, Earl AM, Hadjifrangiskou M, Schmitz J. The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria. Microbiol Spectr [print-electronic]. 2023 Jun 6/15/2023; 11(3): e0471022. PMID: 37195213, PMCID: PMC10269530, DOI: 10.1128/spectrum.04710-22, ISSN: 2165-0497.
Hadjifrangiskou M, Liu J, Ottemann KM, Roujeinikova A. A peek inside the 2023 Bacterial Locomotion and Signal Transduction (BLAST) conference. Trends Microbiol [print-electronic]. 2023 Jun; 31(6): 539-43. PMID: 37127442, PII: S0966-842X(23)00096-3, DOI: 10.1016/j.tim.2023.03.016, ISSN: 1878-4380.
Pilewski KA, Wall S, Richardson SI, Manamela NP, Clark K, Hermanus T, Binshtein E, Venkat R, Sautto GA, Kramer KJ, Shiakolas AR, Setliff I, Salas J, Mapengo RE, Suryadevara N, Brannon JR, Beebout CJ, Parks R, Raju N, Frumento N, Walker LM, Fechter EF, Qin JS, Murji AA, Janowska K, Thakur B, Lindenberger J, May AJ, Huang X, Sammour S, Acharya P, Carnahan RH, Ross TM, Haynes BF, Hadjifrangiskou M, Crowe JE, Bailey JR, Kalams S, Morris L, Georgiev IS. Functional HIV-1/HCV cross-reactive antibodies isolated from a chronically co-infected donor. Cell Rep [print-electronic]. 2023 Feb 2/28/2023; 42(2): 112044. PMID: 36708513, PMCID: PMC10372200, PII: S2211-1247(23)00055-4, DOI: 10.1016/j.celrep.2023.112044, ISSN: 2211-1247.
Wiebe MA, Brannon JR, Steiner BD, Bamidele A, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, Hadjifrangiskou M. Serine Deamination Is a New Acid Tolerance Mechanism Observed in Uropathogenic Escherichia coli. MBio [print-electronic]. 2022 Dec 12/20/2022; 13(6): e0296322. PMID: 36468870, PMCID: PMC9765748, DOI: 10.1128/mbio.02963-22, ISSN: 2150-7511.
Sultana S, Crompton ME, Meurer K, Jankiewicz O, Morales GH, Johnson C, Horbach E, Hoffmann KP, Kr P, Shah R, Anderson GM, Mortimer NT, Schmitz JE, Hadjifrangiskou M, Foti A, Dahl JU. Redox-Mediated Inactivation of the Transcriptional Repressor RcrR is Responsible for Uropathogenic Escherichia coli's Increased Resistance to Reactive Chlorine Species. MBio [print-electronic]. 2022 Oct 10/26/2022; 13(5): e0192622. PMID: 36073817, PMCID: PMC9600549, DOI: 10.1128/mbio.01926-22, ISSN: 2150-7511.
Beebout CJ, Robertson GL, Reinfeld BI, Blee AM, Morales GH, Brannon JR, Chazin WJ, Rathmell WK, Rathmell JC, Gama V, Hadjifrangiskou M. Uropathogenic Escherichia coli subverts mitochondrial metabolism to enable intracellular bacterial pathogenesis in urinary tract infection. Nat Microbiol [print-electronic]. 2022 Sep; 7(9): 1348-60. PMID: 35995841, PMCID: PMC9756876, PII: 10.1038/s41564-022-01205-w, DOI: 10.1038/s41564-022-01205-w, ISSN: 2058-5276.
Fleming BA, Blango MG, Rousek AA, Kincannon WM, Tran A, Lewis AJ, Russell CW, Zhou Q, Baird LM, Barber AE, Brannon JR, Beebout CJ, Bandarian V, Hadjifrangiskou M, Howard MT, Mulvey MA. A tRNA modifying enzyme as a tunable regulatory nexus for bacterial stress responses and virulence. Nucleic Acids Res. 2022 Jul 7/22/2022; 50(13): 7570-90. PMID: 35212379, PMCID: PMC9303304, PII: 6536892, DOI: 10.1093/nar/gkac116, ISSN: 1362-4962.
Steenwyk JL, Buida TJ, Gonçalves C, Goltz DC, Morales G, Mead ME, LaBella AL, Chavez CM, Schmitz JE, Hadjifrangiskou M, Li Y, Rokas A. BioKIT: a versatile toolkit for processing and analyzing diverse types of sequence data. Genetics. 2022 Jul 7/4/2022; 221(3): PMID: 35536198, PMCID: PMC9252278, PII: 6583183, DOI: 10.1093/genetics/iyac079, ISSN: 1943-2631.
Reasoner SA, Enriquez KT, Abelson B, Scaglione S, Schneier B, O'Connor MG, Van Horn G, Hadjifrangiskou M. Urinary tract infections in cystic fibrosis patients. J Cyst Fibros [print-electronic]. 2022 Jan; 21(1): e1-e4-e4. PMID: 34330649, PMCID: PMC9627942, PII: S1569-1993(21)01303-5, DOI: 10.1016/j.jcf.2021.07.005, ISSN: 1873-5010.
Beebout CJ, Sominsky LA, Eberly AR, Van Horn GT, Hadjifrangiskou M. Cytochrome bd promotes Escherichia coli biofilm antibiotic tolerance by regulating accumulation of noxious chemicals. NPJ Biofilms Microbiomes. 2021 Apr 4/16/2021; 7(1): 35. PMID: 33863914, PMCID: PMC8052454, PII: 10.1038/s41522-021-00210-x, DOI: 10.1038/s41522-021-00210-x, ISSN: 2055-5008.
Eberly AR, Beebout CJ, Tong CMC, Van Horn GT, Green HD, Fitzgerald MJ, De S, Apple EK, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, Clayton DB, Stratton CW, Schmitz JE, Hadjifrangiskou M. Data highlighting phenotypic diversity of urine-associated Escherichia coli isolates. Data Brief. 2020 Aug; 31: 105811. PMID: 32566710, PMCID: PMC7298415, PII: S2352-3409(20)30705-8, DOI: 10.1016/j.dib.2020.105811, ISSN: 2352-3409.
Brannon JR, Dunigan TL, Beebout CJ, Ross T, Wiebe MA, Reynolds WS, Hadjifrangiskou M. Invasion of vaginal epithelial cells by uropathogenic Escherichia coli. Nat Commun. 2020 Jun 6/4/2020; 11(1): 2803. PMID: 32499566, PMCID: PMC7272400, PII: 10.1038/s41467-020-16627-5, DOI: 10.1038/s41467-020-16627-5, ISSN: 2041-1723.
Eberly AR, Beebout CJ, Carmen Tong CM, Van Horn GT, Green HD, Fitzgerald MJ, De S, Apple EK, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, Clayton DB, Stratton CW, Schmitz JE, Hadjifrangiskou M. Defining a Molecular Signature for Uropathogenic versus Urocolonizing Escherichia coli: The Status of the Field and New Clinical Opportunities. J Mol Biol [print-electronic]. 2020 Feb 2/14/2020; 432(4): 786-804. PMID: 31794727, PMCID: PMC7293133, PII: S0022-2836(19)30672-2, DOI: 10.1016/j.jmb.2019.11.008, ISSN: 1089-8638.
Beebout CJ, Eberly AR, Werby SH, Reasoner SA, Brannon JR, De S, Fitzgerald MJ, Huggins MM, Clayton DB, Cegelski L, Hadjifrangiskou M. Respiratory Heterogeneity Shapes Biofilm Formation and Host Colonization in Uropathogenic Escherichia coli. MBio. 2019 Apr 4/2/2019; 10(2): PMID: 30940709, PMCID: PMC6445943, PII: mBio.02400-18, DOI: 10.1128/mBio.02400-18, ISSN: 2150-7511.
Magana M, Sereti C, Ioannidis A, Mitchell CA, Ball AR, Magiorkinis E, Chatzipanagiotou S, Hamblin MR, Hadjifrangiskou M, Tegos GP. Options and Limitations in Clinical Investigation of Bacterial Biofilms. Clin Microbiol Rev [electronic-print]. 2018 Jul; 31(3): PMID: 29618576, PMCID: PMC6056845, PII: 31/3/e00084-16, DOI: 10.1128/CMR.00084-16, ISSN: 1098-6618.
Steiner BD, Eberly AR, Hurst MN, Zhang EW, Green HD, Behr S, Jung K, Hadjifrangiskou M. Evidence of Cross-Regulation in Two Closely Related Pyruvate-Sensing Systems in Uropathogenic Escherichia coli. J Membr Biol [print-electronic]. 2018 Feb; 251(1): 65-74. PMID: 29374286, PMCID: PMC5834395, PII: 10.1007/s00232-018-0014-2, DOI: 10.1007/s00232-018-0014-2, ISSN: 1432-1424.
Dale JL, Raynor MJ, Ty MC, Hadjifrangiskou M, Koehler TM. A Dual Role for the Bacillus anthracis Master Virulence Regulator AtxA: Control of Sporulation and Anthrax Toxin Production. Front Microbiol. 2018; 9: 482. PMID: 29599764, PMCID: PMC5862856, DOI: 10.3389/fmicb.2018.00482, ISSN: 1664-302X.
Eberly AR, Floyd KA, Beebout CJ, Colling SJ, Fitzgerald MJ, Stratton CW, Schmitz JE, Hadjifrangiskou M. Biofilm Formation by Uropathogenic Escherichia coli Is Favored under Oxygen Conditions That Mimic the Bladder Environment. Int J Mol Sci. 2017 Sep 9/30/2017; 18(10): PMID: 28973965, PMCID: PMC5666759, PII: ijms18102077, DOI: 10.3390/ijms18102077, ISSN: 1422-0067.
Abana CM, Brannon JR, Ebbott RA, Dunigan TL, Guckes KR, Fuseini H, Powers J, Rogers BR, Hadjifrangiskou M. Characterization of blue light irradiation effects on pathogenic and nonpathogenic Escherichia coli. Microbiologyopen [print-electronic]. 2017 Aug; 6(4): PMID: 28332311, PMCID: PMC5552948, DOI: 10.1002/mbo3.466, ISSN: 2045-8827.
Behr S, Kristoficova I, Witting M, Breland EJ, Eberly AR, Sachs C, Schmitt-Kopplin P, Hadjifrangiskou M, Jung K. Identification of a High-Affinity Pyruvate Receptor in Escherichia coli. Sci Rep. 2017 May 5/3/2017; 7(1): 1388. PMID: 28469239, PMCID: PMC5431176, PII: 10.1038/s41598-017-01410-2, DOI: 10.1038/s41598-017-01410-2, ISSN: 2045-2322.
Breland EJ, Zhang EW, Bermudez T, Martinez CR, Hadjifrangiskou M. The histidine residue of QseC is required for canonical signaling between QseB and PmrB in uropathogenic Escherichia coli. J. Bacteriol [print-electronic]. 2017 Apr 4/10/2017; PMID: 28396353, PII: JB.00060-17, DOI: 10.1128/JB.00060-17, ISSN: 1098-5530.
Guckes KR, Breland EJ, Zhang EW, Hanks SC, Gill NK, Algood HM, Schmitz JE, Stratton CW, Hadjifrangiskou M. Signaling by two-component system noncognate partners promotes intrinsic tolerance to polymyxin B in uropathogenic Escherichia coli. Sci Signal. 2017 Jan 1/10/2017; 10(461): PMID: 28074004, PII: 10/461/eaag1775, DOI: 10.1126/scisignal.aag1775, ISSN: 1937-9145.
Breland EJ, Eberly AR, Hadjifrangiskou M. An Overview of Two-Component Signal Transduction Systems Implicated in Extra-Intestinal Pathogenic E. coli Infections. Front Cell Infect Microbiol. 2017; 7: 162. PMID: 28536675, PMCID: PMC5422438, DOI: 10.3389/fcimb.2017.00162, ISSN: 2235-2988.
Shaffer CL, Zhang EW, Dudley AG, Dixon BR, Guckes KR, Breland EJ, Floyd KA, Casella DP, Algood HM, Clayton DB, Hadjifrangiskou M. Purine Biosynthesis Metabolically Constrains Intracellular Survival of Uropathogenic Escherichia coli. Infect. Immun [electronic-print]. 2017 Jan; 85(1): PMID: 27795353, PMCID: PMC5203662, PII: IAI.00471-16, DOI: 10.1128/IAI.00471-16, ISSN: 1098-5522.
Varga MG, Shaffer CL, Sierra JC, Suarez G, Piazuelo MB, Whitaker ME, Romero-Gallo J, Krishna US, Delgado A, Gomez MA, Good JA, Almqvist F, Skaar EP, Correa P, Wilson KT, Hadjifrangiskou M, Peek RM. Pathogenic Helicobacter pylori strains translocate DNA and activate TLR9 via the cancer-associated cag type IV secretion system. Oncogene [print-electronic]. 2016 Dec 12/1/2016; 35(48): 6262-9. PMID: 27157617, PMCID: PMC5102820, PII: onc2016158, DOI: 10.1038/onc.2016.158, ISSN: 1476-5594.
Floyd KA, Mitchell CA, Eberly AR, Colling SJ, Zhang EW, DePas W, Chapman MR, Conover M, Rogers BR, Hultgren SJ, Hadjifrangiskou M. The UbiI (VisC) Aerobic Ubiquinone Synthase Is Required for Expression of Type 1 Pili, Biofilm Formation, and Pathogenesis in Uropathogenic Escherichia coli. J. Bacteriol [electronic-print]. 2016 Oct 10/1/2016; 198(19): 2662-72. PMID: 27161114, PMCID: PMC5019064, PII: JB.00030-16, DOI: 10.1128/JB.00030-16, ISSN: 1098-5530.
Shaffer CL, Good JA, Kumar S, Krishnan KS, Gaddy JA, Loh JT, Chappell J, Almqvist F, Cover TL, Hadjifrangiskou M. Peptidomimetic Small Molecules Disrupt Type IV Secretion System Activity in Diverse Bacterial Pathogens. MBio. 2016 Apr 4/26/2016; 7(2): e00221-16. PMID: 27118587, PMCID: PMC4850256, PII: mBio.00221-16, DOI: 10.1128/mBio.00221-16, ISSN: 2150-7511.
Conover MS, Hadjifrangiskou M, Palermo JJ, Hibbing ME, Dodson KW, Hultgren SJ. Metabolic Requirements of Escherichia coli in Intracellular Bacterial Communities during Urinary Tract Infection Pathogenesis. MBio. 2016 Apr 4/12/2016; 7(2): e00104-16-e116. PMID: 27073089, PMCID: PMC4959519, PII: mBio.00104-16, DOI: 10.1128/mBio.00104-16, ISSN: 2150-7511.
Brannon JR, Hadjifrangiskou M. The arsenal of pathogens and antivirulence therapeutic strategies for disarming them. Drug Des Devel Ther. 2016; 10: 1795-806. PMID: 27313446, PMCID: PMC4890686, PII: dddt-10-1795, DOI: 10.2147/DDDT.S98939, ISSN: 1177-8881.
Floyd KA, Meyer AE, Nelson G, Hadjifrangiskou M. The yin-yang driving urinary tract infection and how proteomics can enhance research, diagnostics, and treatment. Proteomics Clin Appl [print-electronic]. 2015 Dec; 9(11-12): 990-1002. PMID: 26255866, DOI: 10.1002/prca.201500018, ISSN: 1862-8354.
Floyd KA, Moore JL, Eberly AR, Good JA, Shaffer CL, Zaver H, Almqvist F, Skaar EP, Caprioli RM, Hadjifrangiskou M. Adhesive fiber stratification in uropathogenic Escherichia coli biofilms unveils oxygen-mediated control of type 1 pili. PLoS Pathog. 2015 Mar; 11(3): e1004697. PMID: 25738819, PMCID: PMC4349694, PII: PPATHOGENS-D-14-02041, DOI: 10.1371/journal.ppat.1004697, ISSN: 1553-7374.
Nagamatsu K, Hannan TJ, Guest RL, Kostakioti M, Hadjifrangiskou M, Binkley J, Dodson K, Raivio TL, Hultgren SJ. Dysregulation of Escherichia coli a-hemolysin expression alters the course of acute and persistent urinary tract infection. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Feb 2/24/2015; 112(8): E871-80. PMID: 25675528, PMCID: PMC4345586, PII: 1500374112, DOI: 10.1073/pnas.1500374112, ISSN: 1091-6490.
Greene SE, Pinkner JS, Chorell E, Dodson KW, Shaffer CL, Conover MS, Livny J, Hadjifrangiskou M, Almqvist F, Hultgren SJ. Pilicide ec240 disrupts virulence circuits in uropathogenic Escherichia coli. MBio. 2014; 5(6): e02038. PMID: 25352623, PMCID: PMC4217179, PII: mBio.02038-14, DOI: 10.1128/mBio.02038-14, ISSN: 2150-7511.
Guckes KR, Kostakioti M, Breland EJ, Gu AP, Shaffer CL, Martinez CR, Hultgren SJ, Hadjifrangiskou M. Strong cross-system interactions drive the activation of the QseB response regulator in the absence of its cognate sensor. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Oct 10/8/2013; 110(41): 16592-7. PMID: 24062463, PMCID: PMC3799328, PII: 1315320110, DOI: 10.1073/pnas.1315320110, ISSN: 1091-6490.
Kostakioti M, Hadjifrangiskou M, Hultgren SJ. Bacterial biofilms: development, dispersal, and therapeutic strategies in the dawn of the postantibiotic era. Cold Spring Harb Perspect Med. 2013 Apr; 3(4): a010306. PMID: 23545571, PMCID: PMC3683961, PII: 3/4/a010306, DOI: 10.1101/cshperspect.a010306, ISSN: 2157-1422.
Hung C, Zhou Y, Pinkner JS, Dodson KW, Crowley JR, Heuser J, Chapman MR, Hadjifrangiskou M, Henderson JP, Hultgren SJ. Escherichia coli biofilms have an organized and complex extracellular matrix structure. MBio. 2013; 4(5): e00645-13. PMID: 24023384, PMCID: PMC3774191, PII: mBio.00645-13, DOI: 10.1128/mBio.00645-13, ISSN: 2150-7511.
Kostakioti M, Hultgren SJ, Hadjifrangiskou M. Molecular blueprint of uropathogenic Escherichia coli virulence provides clues toward the development of anti-virulence therapeutics. Virulence [print-electronic]. 2012 Nov 11/15/2012; 3(7): 592-4. PMID: 23154288, PMCID: PMC3545937, PII: 22364, DOI: 10.4161/viru.22364, ISSN: 2150-5608.
Hadjifrangiskou M, Gu AP, Pinkner JS, Kostakioti M, Zhang EW, Greene SE, Hultgren SJ. Transposon mutagenesis identifies uropathogenic Escherichia coli biofilm factors. J. Bacteriol [print-electronic]. 2012 Nov; 194(22): 6195-205. PMID: 22984258, PMCID: PMC3486386, PII: JB.01012-12, DOI: 10.1128/JB.01012-12, ISSN: 1098-5530.
Kostakioti M, Hadjifrangiskou M, Cusumano CK, Hannan TJ, Janetka JW, Hultgren SJ. Distinguishing the contribution of type 1 pili from that of other QseB-misregulated factors when QseC is absent during urinary tract infection. Infect. Immun [print-electronic]. 2012 Aug; 80(8): 2826-34. PMID: 22665375, PMCID: PMC3434567, PII: IAI.00283-12, DOI: 10.1128/IAI.00283-12, ISSN: 1098-5522.
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