Faculty Appointments
Associate Professor of Pediatrics
Associate Professor of Biomedical EngineeringAssociate Professor of Pathology, Microbiology and Immunology
Education
M.D., University of Arkansas, Little Rock, ArkansasPh.D., Microbiology and Immunology, University of Arkansas, Little Rock, ArkansasB.S., University of Arkansas, Fayetteville, Arkansas
Office Address
1161 21st Ave
Nashville, TN 37232
Nashville, TN 37232
Research Description
The major focus of my research is to understand the interaction between host and pathogen during invasive bacterial infections. Specifically, we seek to define the bacterial virulence mechanisms and host defenses employed during osteomyelitis, an invasive infection of bone common in both children and adults. Risk factors and protective immune responses for osteomyelitis are poorly defined, and bone infections are notoriously recalcitrant to antimicrobial therapy due to both pathogen-induced bone destruction and the emergence of multi-drug resistant pathogens. Therefore, we have created new tools to investigate the host-pathogen interface during osteomyelitis. As Staphylococcus aureus is by far the most common cause of osteomyelitis, we have focused our efforts on identifying the mechanisms by which S. aureus causes bone infection and resulting bone destruction, as well as the host defenses that protect against staphylococcal infection. Using a combination of bone cell culture models, proteomic analyses of bacterial virulence factors, and a new quantitative in vivo model of staphylococcal osteomyelitis, we have uncovered specific bacterial factors that contribute to the pathogenesis of osteomyelitis by triggering osteoblast cell death and bone destruction in vivo. We have also identified staphylococcal genes necessary for survival within the bone. By further defining the mechanisms by which S. aureus survives within bone and ultimately triggers bone destruction, we hope to identify new therapeutic targets to treat osteomyelitis and to limit the morbidity associated with this invasive infection. Moreover, by characterizing the host responses to bacterial pathogens in the bone, we seek to define protective correlates of innate immunity in bone and potentially uncover risk factors for the development of osteomyelitis in otherwise healthy children. Finally, by investigating how bacterial pathogens perturb bone cell physiology, we wish to enhance an understanding of changes in bone remodeling in the face of infectious and inflammatory insults.
Emerging research areas in the lab also include: 1) Mechanisms of bone loss during systemic inflammatory disorders like inflammatory bowel disease, 2) Investigation of microbe-microbe interactions during polymicrobial infection; and 3) investigation of osteo-immunologic crosstalk during infectious diseases
Emerging research areas in the lab also include: 1) Mechanisms of bone loss during systemic inflammatory disorders like inflammatory bowel disease, 2) Investigation of microbe-microbe interactions during polymicrobial infection; and 3) investigation of osteo-immunologic crosstalk during infectious diseases
Research Keywords
Bacterial pathogenesis; Host-pathogen interactions; osteomyelitis; bone biology; In vivo imaging; osteoblast; osteoclast; pattern recognition receptor; osteoimmunology; Staphylococcus aureus; Salmonella typhimurium; Candida albicans; virulence; animal models; imaging; rheumatology; microbiome; microbial ecology; inflammatory bowel disease
Clinical Research Keywords
Pediatric Infectious Diseases; Bone infections; osteomyelitis; invasive staphylococcal infection
Publications
Ariëns RAS, Cassat JE. Surviving a sticky situation: therapeutic administration of fibrinogen variant ¿' improves outcomes of Staphylococcus aureus septicemia. J Thromb Haemost. 2023 Aug; 21(8): 2048-50. PMID: 37468174, PII: S1538-7836(23)00331-8, DOI: 10.1016/j.jtha.2023.04.013, ISSN: 1538-7836.
Tao L, Foster P, Russo C, Cassat JE, Humphries RM. The Brief Case: Invasive Ceftriaxone-Resistant Nontyphoidal Salmonella and Antimicrobial Susceptibility Testing Considerations. J Clin Microbiol [print-electronic]. 2023 May 5/23/2023; 61(5): e0075022. PMID: 37219092, PMCID: PMC10204636, DOI: 10.1128/jcm.00750-22, ISSN: 1098-660X.
Butrico CE, Klopfenstein N, Green ER, Johnson JR, Peck SH, Ibberson CB, Serezani CH, Cassat JE. Hyperglycemia Increases Severity of Staphylococcus aureus Osteomyelitis and Influences Bacterial Genes Required for Survival in Bone. Infect Immun [print-electronic]. 2023 Apr 4/18/2023; 91(4): e0052922. PMID: 36877063, PMCID: PMC10112148, DOI: 10.1128/iai.00529-22, ISSN: 1098-5522.
Jacobse J, Brown RE, Li J, Pilat JM, Pham L, Short SP, Peek CT, Rolong A, Washington MK, Martinez-Barricarte R, Byndloss MX, Shelton C, Markle JG, Latour YL, Allaman MM, Cassat JE, Wilson KT, Choksi YA, Williams CS, Lau KS, Flynn CR, Casanova JL, Rings EHHM, Samsom JN, Goettel JA. Interleukin-23 receptor signaling impairs the stability and function of colonic regulatory T cells. Cell Rep [print-electronic]. 2023 Feb 2/17/2023; 42(2): 112128. PMID: 36807140, PII: S2211-1247(23)00139-0, DOI: 10.1016/j.celrep.2023.112128, ISSN: 2211-1247.
Gehrke AE, Mendoza-Bertelli A, Ledo C, Gonzalez CD, Noto Llana M, Blanco C, Sordelli DO, Putman NE, Cassat JE, Delpino MV, Gómez MI. Neutralization of Staphylococcus aureus Protein A Prevents Exacerbated Osteoclast Activity and Bone Loss during Osteomyelitis. Antimicrob Agents Chemother [print-electronic]. 2023 Jan 1/24/2023; 67(1): e0114022. PMID: 36533935, PMCID: PMC9872667, DOI: 10.1128/aac.01140-22, ISSN: 1098-6596.
Moriarty TF, Metsemakers WJ, Morgenstern M, Hofstee MI, Vallejo Diaz A, Cassat JE, Wildemann B, Depypere M, Schwarz EM, Richards RG. Fracture-related infection. Nat Rev Dis Primers. 2022 Oct 10/20/2022; 8(1): 67. PMID: 36266296, PII: 10.1038/s41572-022-00396-0, DOI: 10.1038/s41572-022-00396-0, ISSN: 2056-676X.
Petronglo JR, Putnam NE, Ford CA, Cruz-Victorio V, Curry JM, Butrico CE, Fulbright LE, Johnson JR, Peck SH, Fatah SR, Cassat JE. Context-Dependent Roles for Toll-Like Receptors 2 and 9 in the Pathogenesis of Staphylococcus aureus Osteomyelitis. Infect Immun [print-electronic]. 2022 Oct 10/13/2022; e0041722. PMID: 36226943, DOI: 10.1128/iai.00417-22, ISSN: 1098-5522.
Perry WJ, Grunenwald CM, Van de Plas R, Witten JC, Martin DR, Apte SS, Cassat JE, Pettersson GB, Caprioli RM, Skaar EP, Spraggins JM. Visualizing Staphylococcus aureus pathogenic membrane modification within the host infection environment by multimodal imaging mass spectrometry. Cell Chem Biol [print-electronic]. 2022 Jul 7/21/2022; 29(7): 1209-1217.e4. PMID: 35654040, PMCID: PMC9308753, PII: S2451-9456(22)00196-9, DOI: 10.1016/j.chembiol.2022.05.004, ISSN: 2451-9448.
Good CJ, Neumann EK, Butrico CE, Cassat JE, Caprioli RM, Spraggins JM. High Spatial Resolution MALDI Imaging Mass Spectrometry of Fresh-Frozen Bone. Anal Chem [print-electronic]. 2022 Feb 2/22/2022; 94(7): 3165-72. PMID: 35138834, DOI: 10.1021/acs.analchem.1c04604, ISSN: 1520-6882.
Peek CT, Ford CA, Eichelberger KR, Jacobse J, Torres TP, Maseda D, Latour YL, Piazuelo MB, Johnson JR, Byndloss MX, Wilson KT, Rathmell JC, Goettel JA, Cassat JE. Intestinal Inflammation Promotes MDL-1+ Osteoclast Precursor Expansion to Trigger Osteoclastogenesis and Bone Loss. Cell Mol Gastroenterol Hepatol [print-electronic]. 2022; 14(4): 731-50. PMID: 35835390, PMCID: PMC9420375, PII: S2352-345X(22)00160-6, DOI: 10.1016/j.jcmgh.2022.07.002, ISSN: 2352-345X.
Ford CA, Hurford IM, Fulbright LE, Curry JM, Peek CT, Spoonmore TJ, Cruz Victorio V, Johnson JR, Peck SH, Cassat JE. Loss of Vhl alters trabecular bone loss during S. aureus osteomyelitis in a cell-specific manner. Front Cell Infect Microbiol. 2022; 12: 985467. PMID: 36204648, PMCID: PMC9530664, DOI: 10.3389/fcimb.2022.985467, ISSN: 2235-2988.
Cassat JE, Thomsen I. Staphylococcus aureus infections in children. Curr Opin Infect Dis. 2021 Oct 10/1/2021; 34(5): 510-8. PMID: 34524201, PMCID: PMC8630804, PII: 00001432-202110000-00018, DOI: 10.1097/QCO.0000000000000752, ISSN: 1473-6527.
Roper PM, Eichelberger KR, Cox L, O'Connor L, Shao C, Ford CA, Fritz SA, Cassat JE, Veis DJ. Contemporary Clinical Isolates of Staphylococcus aureus from Pediatric Osteomyelitis Patients Display Unique Characteristics in a Mouse Model of Hematogenous Osteomyelitis. Infect Immun [print-electronic]. 2021 Sep 9/16/2021; 89(10): e0018021. PMID: 34097469, PMCID: PMC8445171, DOI: 10.1128/IAI.00180-21, ISSN: 1098-5522.
Veis DJ, Cassat JE. Infectious Osteomyelitis: Marrying Bone Biology and Microbiology to Shed New Light on a Persistent Clinical Challenge. J Bone Miner Res [print-electronic]. 2021 Apr; 36(4): 636-43. PMID: 33740314, DOI: 10.1002/jbmr.4279, ISSN: 1523-4681.
Klopfenstein N, Cassat JE, Monteith A, Miller A, Drury S, Skaar E, Serezani CH. Murine Models for Staphylococcal Infection. Curr Protoc. 2021 Mar; 1(3): e52. PMID: 33656290, PMCID: PMC7935403, DOI: 10.1002/cpz1.52, ISSN: 2691-1299.
Ford CA, Spoonmore TJ, Gupta MK, Duvall CL, Guelcher SA, Cassat JE. Diflunisal-loaded poly(propylene sulfide) nanoparticles decrease S. aureus-mediated bone destruction during osteomyelitis. J Orthop Res [print-electronic]. 2021 Feb; 39(2): 426-37. PMID: 33300149, PMCID: PMC7855846, DOI: 10.1002/jor.24948, ISSN: 1554-527X.
Gimza BD, Cassat JE. Mechanisms of Antibiotic Failure During Staphylococcus aureus Osteomyelitis. Front Immunol. 2021; 12: 638085. PMID: 33643322, PMCID: PMC7907425, DOI: 10.3389/fimmu.2021.638085, ISSN: 1664-3224.
Ford CA, Cassat JE. Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted Toxins. Methods Mol Biol. 2021; 2341: 141-52. PMID: 34264470, DOI: 10.1007/978-1-0716-1550-8_17, ISSN: 1940-6029.
Eichelberger KR, Cassat JE. Metabolic Adaptations During Staphylococcus aureus and Candida albicans Co-Infection. Front Immunol. 2021; 12: 797550. PMID: 34956233, PMCID: PMC8692374, DOI: 10.3389/fimmu.2021.797550, ISSN: 1664-3224.
Butrico CE, Cassat JE. Quorum Sensing and Toxin Production in Staphylococcus aureus Osteomyelitis: Pathogenesis and Paradox. Toxins (Basel). 2020 Aug 8/12/2020; 12(8): PMID: 32806558, PMCID: PMC7471978, PII: toxins12080516, DOI: 10.3390/toxins12080516, ISSN: 2072-6651.
Spoonmore TJ, Ford CA, Curry JM, Guelcher SA, Cassat JE. Concurrent Local Delivery of Diflunisal Limits Bone Destruction but Fails To Improve Systemic Vancomycin Efficacy during Staphylococcus aureus Osteomyelitis. Antimicrob Agents Chemother [electronic-print]. 2020 Jun 6/23/2020; 64(7): PMID: 32340992, PMCID: PMC7318050, PII: AAC.00182-20, DOI: 10.1128/AAC.00182-20, ISSN: 1098-6596.
Urish KL, Cassat JE. Staphylococcus aureus Osteomyelitis: Bone, Bugs, and Surgery. Infect Immun [electronic-print]. 2020 Jun 6/22/2020; 88(7): PMID: 32094258, PMCID: PMC7309607, PII: IAI.00932-19, DOI: 10.1128/IAI.00932-19, ISSN: 1098-5522.
Potter AD, Butrico CE, Ford CA, Curry JM, Trenary IA, Tummarakota SS, Hendrix AS, Young JD, Cassat JE. Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection. Proc Natl Acad Sci U S A [print-electronic]. 2020 Jun 6/2/2020; 117(22): 12394-401. PMID: 32414924, PMCID: PMC7275739, PII: 1922211117, DOI: 10.1073/pnas.1922211117, ISSN: 1091-6490.
Peek CT, Ibberson CB, Cassat JE. Identification of Virulence Determinants During Host-Pathogen Interaction Using Tn-Seq Technology. Methods Mol Biol. 2020; 2069: 155-75. PMID: 31523773, DOI: 10.1007/978-1-4939-9849-4_12, ISSN: 1940-6029.
Ford CA, Hurford IM, Cassat JE. Antivirulence Strategies for the Treatment of Staphylococcus aureus Infections: A Mini Review. Front Microbiol. 2020; 11: 632706. PMID: 33519793, PMCID: PMC7840885, DOI: 10.3389/fmicb.2020.632706, ISSN: 1664-302X.
Perry WJ, Spraggins JM, Sheldon JR, Grunenwald CM, Heinrichs DE, Cassat JE, Skaar EP, Caprioli RM. Staphylococcus aureus exhibits heterogeneous siderophore production within the vertebrate host. Proc Natl Acad Sci U S A [print-electronic]. 2019 Oct 10/29/2019; 116(44): 21980-2. PMID: 31611408, PMCID: PMC6825271, PII: 1913991116, DOI: 10.1073/pnas.1913991116, ISSN: 1091-6490.
Krauss JL, Roper PM, Ballard A, Shih CC, Fitzpatrick JAJ, Cassat JE, Ng PY, Pavlos NJ, Veis DJ. Staphylococcus aureus Infects Osteoclasts and Replicates Intracellularly. MBio. 2019 Oct 10/15/2019; 10(5): PMID: 31615966, PMCID: PMC6794488, PII: mBio.02447-19, DOI: 10.1128/mBio.02447-19, ISSN: 2150-7511.
Ryan DJ, Patterson NH, Putnam NE, Wilde AD, Weiss A, Perry WJ, Cassat JE, Skaar EP, Caprioli RM, Spraggins JM. MicroLESA: Integrating Autofluorescence Microscopy, In Situ Micro-Digestions, and Liquid Extraction Surface Analysis for High Spatial Resolution Targeted Proteomic Studies. Anal. Chem [print-electronic]. 2019 Jun 6/18/2019; 91(12): 7578-85. PMID: 31149808, DOI: 10.1021/acs.analchem.8b05889, ISSN: 1520-6882.
Putnam NE, Fulbright LE, Curry JM, Ford CA, Petronglo JR, Hendrix AS, Cassat JE. MyD88 and IL-1R signaling drive antibacterial immunity and osteoclast-driven bone loss during Staphylococcus aureus osteomyelitis. PLoS Pathog. 2019 Apr; 15(4): e1007744. PMID: 30978245, PMCID: PMC6481883, PII: PPATHOGENS-D-18-02431, DOI: 10.1371/journal.ppat.1007744, ISSN: 1553-7374.
Buenrostro D, Kwakwa KA, Putnam NE, Merkel AR, Johnson JR, Cassat JE, Sterling JA. Early TGF-ß inhibition in mice reduces the incidence of breast cancer induced bone disease in a myeloid dependent manner. Bone [print-electronic]. 2018 Aug; 113: 77-88. PMID: 29753718, PMCID: PMC6118216, PII: S8756-3282(18)30187-X, DOI: 10.1016/j.bone.2018.05.008, ISSN: 1873-2763.
Buenrostro D, Kwakwa KA, Putnam NE, Merkel AR, Johnson JR, Cassat JE, Sterling JA. Early TGF-ß inhibition in mice reduces the incidence of breast cancer induced bone disease in a myeloid dependent manner. Bone [print-electronic]. 2018 Aug; 113: 77-88. PMID: 29753718, PMCID: PMC6118216, PII: S8756-3282(18)30187-X, DOI: 10.1016/j.bone.2018.05.008, ISSN: 1873-2763.
Brandt SL, Putnam NE, Cassat JE, Serezani CH. Innate Immunity to Staphylococcus aureus: Evolving Paradigms in Soft Tissue and Invasive Infections. J. Immunol. 2018 Jun 6/15/2018; 200(12): 3871-80. PMID: 29866769, PMCID: PMC6028009, PII: 200/12/3871, DOI: 10.4049/jimmunol.1701574, ISSN: 1550-6606.
Brandt SL, Putnam NE, Cassat JE, Serezani CH. Innate Immunity to Staphylococcus aureus: Evolving Paradigms in Soft Tissue and Invasive Infections. J. Immunol. 2018 Jun 6/15/2018; 200(12): 3871-80. PMID: 29866769, PMCID: PMC6028009, PII: 200/12/3871, DOI: 10.4049/jimmunol.1701574, ISSN: 1550-6606.
Cassat JE, Moore JL, Wilson KJ, Stark Z, Prentice BM, Van de Plas R, Perry WJ, Zhang Y, Virostko J, Colvin DC, Rose KL, Judd AM, Reyzer ML, Spraggins JM, Grunenwald CM, Gore JC, Caprioli RM, Skaar EP. Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions. Sci Transl Med. 2018 Mar 3/14/2018; 10(432): PMID: 29540616, PMCID: PMC6005374, PII: 10/432/eaan6361, DOI: 10.1126/scitranslmed.aan6361, ISSN: 1946-6242.
Cassat JE, Moore JL, Wilson KJ, Stark Z, Prentice BM, Van de Plas R, Perry WJ, Zhang Y, Virostko J, Colvin DC, Rose KL, Judd AM, Reyzer ML, Spraggins JM, Grunenwald CM, Gore JC, Caprioli RM, Skaar EP. Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions. Sci Transl Med. 2018 Mar 3/14/2018; 10(432): PMID: 29540616, PMCID: PMC6005374, PII: 10/432/eaan6361, DOI: 10.1126/scitranslmed.aan6361, ISSN: 1946-6242.
Frazier SB, Katz S, Wood JB, Cassat JE. An Unusual Source of Sepsis in Two Previously Healthy Children. Clin Pediatr (Phila) [print-electronic]. 2017 Oct 10/1/2017; 9922817738349. PMID: 29084446, DOI: 10.1177/0009922817738349, ISSN: 1938-2707.
Ford CA, Cassat JE. Advances in the local and targeted delivery of anti-infective agents for management of osteomyelitis. Expert Rev Anti Infect Ther [print-electronic]. 2017 Sep; 15(9): 851-60. PMID: 28837368, DOI: 10.1080/14787210.2017.1372192, ISSN: 1744-8336.
Thomsen IP, Sapparapu G, James DB, Cassat JE, Nagarsheth M, Kose N, Putnam N, Boguslawski KM, Jones LS, Wood JB, Creech CB, Torres VJ, Crowe JE. Monoclonal antibodies against the Staphylococcus aureus bicomponent leukotoxin AB (LukAB) isolated following invasive human infection reveal diverse binding and modes of action. J. Infect. Dis [print-electronic]. 2017 Feb 2/10/2017; PMID: 28186295, PII: 2982058, DOI: 10.1093/infdis/jix071, ISSN: 1537-6613.
Mendoza Bertelli A, Delpino MV, Lattar S, Giai C, Llana MN, Sanjuan N, Cassat JE, Sordelli D, Gómez MI. Staphylococcus aureus protein A enhances osteoclastogenesis via TNFR1 and EGFR signaling. Biochim. Biophys. Acta [print-electronic]. 2016 Oct; 1862(10): 1975-83. PMID: 27475257, PII: S0925-4439(16)30184-3, DOI: 10.1016/j.bbadis.2016.07.016, ISSN: 0006-3002.
Hendrix AS, Spoonmore TJ, Wilde AD, Putnam NE, Hammer ND, Snyder DJ, Guelcher SA, Skaar EP, Cassat JE. Repurposing the Nonsteroidal Anti-inflammatory Drug Diflunisal as an Osteoprotective, Antivirulence Therapy for Staphylococcus aureus Osteomyelitis. Antimicrob. Agents Chemother [electronic-print]. 2016 Sep; 60(9): 5322-30. PMID: 27324764, PMCID: PMC4997817, PII: AAC.00834-16, DOI: 10.1128/AAC.00834-16, ISSN: 1098-6596.
Loughran AJ, Gaddy D, Beenken KE, Meeker DG, Morello R, Zhao H, Byrum SD, Tackett AJ, Cassat JE, Smeltzer MS. Impact of sarA and Phenol-Soluble Modulins on the Pathogenesis of Osteomyelitis in Diverse Clinical Isolates of Staphylococcus aureus. Infect. Immun [electronic-print]. 2016 Sep; 84(9): 2586-94. PMID: 27354444, PMCID: PMC4995912, PII: IAI.00152-16, DOI: 10.1128/IAI.00152-16, ISSN: 1098-5522.
Wilde AD, Snyder DJ, Putnam NE, Valentino MD, Hammer ND, Lonergan ZR, Hinger SA, Aysanoa EE, Blanchard C, Dunman PM, Wasserman GA, Chen J, Shopsin B, Gilmore MS, Skaar EP, Cassat JE. Bacterial Hypoxic Responses Revealed as Critical Determinants of the Host-Pathogen Outcome by TnSeq Analysis of Staphylococcus aureus Invasive Infection. PLoS Pathog. 2015 Dec; 11(12): e1005341. PMID: 26684646, PMCID: PMC4684308, PII: PPATHOGENS-D-15-02340, DOI: 10.1371/journal.ppat.1005341, ISSN: 1553-7374.
Hammer ND, Cassat JE, Noto MJ, Lojek LJ, Chadha AD, Schmitz JE, Creech CB, Skaar EP. Inter- and intraspecies metabolite exchange promotes virulence of antibiotic-resistant Staphylococcus aureus. Cell Host Microbe. 2014 Oct 10/8/2014; 16(4): 531-7. PMID: 25299336, PMCID: PMC4197139, PII: S1931-3128(14)00335-7, DOI: 10.1016/j.chom.2014.09.002, ISSN: 1934-6069.
Gillon JE, Cassat JE, Di Pentima MC. Validation of two vancomycin nomograms in patients 10 years of age and older. J Clin Pharmacol [print-electronic]. 2014 Jan; 54(1): 35-8. PMID: 24249098, DOI: 10.1002/jcph.173, ISSN: 1552-4604.
Cassat JE, Smeltzer MS, Lee CY. Investigation of biofilm formation in clinical isolates of Staphylococcus aureus. Methods Mol. Biol. 2014; 1085: 195-211. PMID: 24085698, DOI: 10.1007/978-1-62703-664-1_12, ISSN: 1940-6029.
Cassat JE, Skaar EP. Recent advances in experimental models of osteomyelitis [editorial]. Expert Rev Anti Infect Ther. 2013 Dec; 11(12): 1263-5. PMID: 24215241, DOI: 10.1586/14787210.2013.858600, ISSN: 1744-8336.
Attia AS, Cassat JE, Aranmolate SO, Zimmerman LJ, Boyd KL, Skaar EP. Analysis of the Staphylococcus aureus abscess proteome identifies antimicrobial host proteins and bacterial stress responses at the host-pathogen interface. Pathog Dis [print-electronic]. 2013 Jul 7/11/2013; PMID: 23847107, PMCID: PMC3877740, DOI: 10.1111/2049-632X.12063, ISSN: 2049-632X.
Cassat JE, Hammer ND, Campbell JP, Benson MA, Perrien DS, Mrak LN, Smeltzer MS, Torres VJ, Skaar EP. A secreted bacterial protease tailors the Staphylococcus aureus virulence repertoire to modulate bone remodeling during osteomyelitis. Cell Host Microbe. 2013 Jun 6/12/2013; 13(6): 759-72. PMID: 23768499, PMCID: PMC3721972, PII: S1931-3128(13)00185-6, DOI: 10.1016/j.chom.2013.05.003, ISSN: 1934-6069.
Cassat JE, Skaar EP. Iron in infection and immunity. Cell Host Microbe. 2013 May 5/15/2013; 13(5): 509-19. PMID: 23684303, PMCID: PMC3676888, PII: S1931-3128(13)00152-2, DOI: 10.1016/j.chom.2013.04.010, ISSN: 1934-6069.
Hammer ND, Reniere ML, Cassat JE, Zhang Y, Hirsch AO, Indriati Hood M, Skaar EP. Two heme-dependent terminal oxidases power Staphylococcus aureus organ-specific colonization of the vertebrate host. MBio. 2013; 4(4): PMID: 23900169, PMCID: PMC3735196, PII: mBio.00241-13, DOI: 10.1128/mBio.00241-13, ISSN: 2150-7511.
Cassat JE, Skaar EP. Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity. Semin Immunopathol [print-electronic]. 2012 Mar; 34(2): 215-35. PMID: 22048835, PMCID: PMC3796439, DOI: 10.1007/s00281-011-0294-4, ISSN: 1863-2300.
Stenz L, François P, Fischer A, Huyghe A, Tangomo M, Hernandez D, Cassat J, Linder P, Schrenzel J. Impact of oleic acid (cis-9-octadecenoic acid) on bacterial viability and biofilm production in Staphylococcus aureus. FEMS Microbiol. Lett [print-electronic]. 2008 Oct; 287(2): 149-55. PMID: 18754790, PII: FML1316, DOI: 10.1111/j.1574-6968.2008.01316.x, ISSN: 0378-1097.
Tsang LH, Cassat JE, Shaw LN, Beenken KE, Smeltzer MS. Factors contributing to the biofilm-deficient phenotype of Staphylococcus aureus sarA mutants. PLoS ONE [print-electronic]. 2008; 3(10): e3361. PMID: 18846215, PMCID: PMC2556392, DOI: 10.1371/journal.pone.0003361, ISSN: 1932-6203.
Rice KC, Mann EE, Endres JL, Weiss EC, Cassat JE, Smeltzer MS, Bayles KW. The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2007 May 5/8/2007; 104(19): 8113-8. PMID: 17452642, PMCID: PMC1876580, PII: 0610226104, DOI: 10.1073/pnas.0610226104, ISSN: 0027-8424.
Cassat JE, Lee CY, Smeltzer MS. Investigation of biofilm formation in clinical isolates of Staphylococcus aureus. Methods Mol. Biol. 2007; 391: 127-44. PMID: 18025674, PMCID: PMC4098860, PII: 1-59745-468-0:127, DOI: 10.1007/978-1-59745-468-1_10, ISSN: 1064-3745.
Cassat J, Dunman PM, Murphy E, Projan SJ, Beenken KE, Palm KJ, Yang SJ, Rice KC, Bayles KW, Smeltzer MS. Transcriptional profiling of a Staphylococcus aureus clinical isolate and its isogenic agr and sarA mutants reveals global differences in comparison to the laboratory strain RN6390. Microbiology (Reading, Engl.). 2006 Oct; 152(Pt 10): 3075-90. PMID: 17005987, PII: 152/10/3075, DOI: 10.1099/mic.0.29033-0, ISSN: 1350-0872.
Cassat JE, Dunman PM, McAleese F, Murphy E, Projan SJ, Smeltzer MS. Comparative genomics of Staphylococcus aureus musculoskeletal isolates. J. Bacteriol. 2005 Jan; 187(2): 576-92. PMID: 15629929, PMCID: PMC543526, PII: 187/2/576, DOI: 10.1128/JB.187.2.576-592.2005, ISSN: 0021-9193.
Kaiser JR, Cassat JE, Lewno MJ. Should antibiotics be discontinued at 48 hours for negative late-onset sepsis evaluations in the neonatal intensive care unit?. J Perinatol. 2002 Sep; 22(6): 445-7. PMID: 12168120, DOI: 10.1038/sj.jp.7210764, ISSN: 0743-8346.
Tao L, Foster P, Russo C, Cassat JE, Humphries RM. The Brief Case: Invasive Ceftriaxone-Resistant Nontyphoidal Salmonella and Antimicrobial Susceptibility Testing Considerations. J Clin Microbiol [print-electronic]. 2023 May 5/23/2023; 61(5): e0075022. PMID: 37219092, PMCID: PMC10204636, DOI: 10.1128/jcm.00750-22, ISSN: 1098-660X.
Butrico CE, Klopfenstein N, Green ER, Johnson JR, Peck SH, Ibberson CB, Serezani CH, Cassat JE. Hyperglycemia Increases Severity of Staphylococcus aureus Osteomyelitis and Influences Bacterial Genes Required for Survival in Bone. Infect Immun [print-electronic]. 2023 Apr 4/18/2023; 91(4): e0052922. PMID: 36877063, PMCID: PMC10112148, DOI: 10.1128/iai.00529-22, ISSN: 1098-5522.
Jacobse J, Brown RE, Li J, Pilat JM, Pham L, Short SP, Peek CT, Rolong A, Washington MK, Martinez-Barricarte R, Byndloss MX, Shelton C, Markle JG, Latour YL, Allaman MM, Cassat JE, Wilson KT, Choksi YA, Williams CS, Lau KS, Flynn CR, Casanova JL, Rings EHHM, Samsom JN, Goettel JA. Interleukin-23 receptor signaling impairs the stability and function of colonic regulatory T cells. Cell Rep [print-electronic]. 2023 Feb 2/17/2023; 42(2): 112128. PMID: 36807140, PII: S2211-1247(23)00139-0, DOI: 10.1016/j.celrep.2023.112128, ISSN: 2211-1247.
Gehrke AE, Mendoza-Bertelli A, Ledo C, Gonzalez CD, Noto Llana M, Blanco C, Sordelli DO, Putman NE, Cassat JE, Delpino MV, Gómez MI. Neutralization of Staphylococcus aureus Protein A Prevents Exacerbated Osteoclast Activity and Bone Loss during Osteomyelitis. Antimicrob Agents Chemother [print-electronic]. 2023 Jan 1/24/2023; 67(1): e0114022. PMID: 36533935, PMCID: PMC9872667, DOI: 10.1128/aac.01140-22, ISSN: 1098-6596.
Moriarty TF, Metsemakers WJ, Morgenstern M, Hofstee MI, Vallejo Diaz A, Cassat JE, Wildemann B, Depypere M, Schwarz EM, Richards RG. Fracture-related infection. Nat Rev Dis Primers. 2022 Oct 10/20/2022; 8(1): 67. PMID: 36266296, PII: 10.1038/s41572-022-00396-0, DOI: 10.1038/s41572-022-00396-0, ISSN: 2056-676X.
Petronglo JR, Putnam NE, Ford CA, Cruz-Victorio V, Curry JM, Butrico CE, Fulbright LE, Johnson JR, Peck SH, Fatah SR, Cassat JE. Context-Dependent Roles for Toll-Like Receptors 2 and 9 in the Pathogenesis of Staphylococcus aureus Osteomyelitis. Infect Immun [print-electronic]. 2022 Oct 10/13/2022; e0041722. PMID: 36226943, DOI: 10.1128/iai.00417-22, ISSN: 1098-5522.
Perry WJ, Grunenwald CM, Van de Plas R, Witten JC, Martin DR, Apte SS, Cassat JE, Pettersson GB, Caprioli RM, Skaar EP, Spraggins JM. Visualizing Staphylococcus aureus pathogenic membrane modification within the host infection environment by multimodal imaging mass spectrometry. Cell Chem Biol [print-electronic]. 2022 Jul 7/21/2022; 29(7): 1209-1217.e4. PMID: 35654040, PMCID: PMC9308753, PII: S2451-9456(22)00196-9, DOI: 10.1016/j.chembiol.2022.05.004, ISSN: 2451-9448.
Good CJ, Neumann EK, Butrico CE, Cassat JE, Caprioli RM, Spraggins JM. High Spatial Resolution MALDI Imaging Mass Spectrometry of Fresh-Frozen Bone. Anal Chem [print-electronic]. 2022 Feb 2/22/2022; 94(7): 3165-72. PMID: 35138834, DOI: 10.1021/acs.analchem.1c04604, ISSN: 1520-6882.
Peek CT, Ford CA, Eichelberger KR, Jacobse J, Torres TP, Maseda D, Latour YL, Piazuelo MB, Johnson JR, Byndloss MX, Wilson KT, Rathmell JC, Goettel JA, Cassat JE. Intestinal Inflammation Promotes MDL-1+ Osteoclast Precursor Expansion to Trigger Osteoclastogenesis and Bone Loss. Cell Mol Gastroenterol Hepatol [print-electronic]. 2022; 14(4): 731-50. PMID: 35835390, PMCID: PMC9420375, PII: S2352-345X(22)00160-6, DOI: 10.1016/j.jcmgh.2022.07.002, ISSN: 2352-345X.
Ford CA, Hurford IM, Fulbright LE, Curry JM, Peek CT, Spoonmore TJ, Cruz Victorio V, Johnson JR, Peck SH, Cassat JE. Loss of Vhl alters trabecular bone loss during S. aureus osteomyelitis in a cell-specific manner. Front Cell Infect Microbiol. 2022; 12: 985467. PMID: 36204648, PMCID: PMC9530664, DOI: 10.3389/fcimb.2022.985467, ISSN: 2235-2988.
Cassat JE, Thomsen I. Staphylococcus aureus infections in children. Curr Opin Infect Dis. 2021 Oct 10/1/2021; 34(5): 510-8. PMID: 34524201, PMCID: PMC8630804, PII: 00001432-202110000-00018, DOI: 10.1097/QCO.0000000000000752, ISSN: 1473-6527.
Roper PM, Eichelberger KR, Cox L, O'Connor L, Shao C, Ford CA, Fritz SA, Cassat JE, Veis DJ. Contemporary Clinical Isolates of Staphylococcus aureus from Pediatric Osteomyelitis Patients Display Unique Characteristics in a Mouse Model of Hematogenous Osteomyelitis. Infect Immun [print-electronic]. 2021 Sep 9/16/2021; 89(10): e0018021. PMID: 34097469, PMCID: PMC8445171, DOI: 10.1128/IAI.00180-21, ISSN: 1098-5522.
Veis DJ, Cassat JE. Infectious Osteomyelitis: Marrying Bone Biology and Microbiology to Shed New Light on a Persistent Clinical Challenge. J Bone Miner Res [print-electronic]. 2021 Apr; 36(4): 636-43. PMID: 33740314, DOI: 10.1002/jbmr.4279, ISSN: 1523-4681.
Klopfenstein N, Cassat JE, Monteith A, Miller A, Drury S, Skaar E, Serezani CH. Murine Models for Staphylococcal Infection. Curr Protoc. 2021 Mar; 1(3): e52. PMID: 33656290, PMCID: PMC7935403, DOI: 10.1002/cpz1.52, ISSN: 2691-1299.
Ford CA, Spoonmore TJ, Gupta MK, Duvall CL, Guelcher SA, Cassat JE. Diflunisal-loaded poly(propylene sulfide) nanoparticles decrease S. aureus-mediated bone destruction during osteomyelitis. J Orthop Res [print-electronic]. 2021 Feb; 39(2): 426-37. PMID: 33300149, PMCID: PMC7855846, DOI: 10.1002/jor.24948, ISSN: 1554-527X.
Gimza BD, Cassat JE. Mechanisms of Antibiotic Failure During Staphylococcus aureus Osteomyelitis. Front Immunol. 2021; 12: 638085. PMID: 33643322, PMCID: PMC7907425, DOI: 10.3389/fimmu.2021.638085, ISSN: 1664-3224.
Ford CA, Cassat JE. Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted Toxins. Methods Mol Biol. 2021; 2341: 141-52. PMID: 34264470, DOI: 10.1007/978-1-0716-1550-8_17, ISSN: 1940-6029.
Eichelberger KR, Cassat JE. Metabolic Adaptations During Staphylococcus aureus and Candida albicans Co-Infection. Front Immunol. 2021; 12: 797550. PMID: 34956233, PMCID: PMC8692374, DOI: 10.3389/fimmu.2021.797550, ISSN: 1664-3224.
Butrico CE, Cassat JE. Quorum Sensing and Toxin Production in Staphylococcus aureus Osteomyelitis: Pathogenesis and Paradox. Toxins (Basel). 2020 Aug 8/12/2020; 12(8): PMID: 32806558, PMCID: PMC7471978, PII: toxins12080516, DOI: 10.3390/toxins12080516, ISSN: 2072-6651.
Spoonmore TJ, Ford CA, Curry JM, Guelcher SA, Cassat JE. Concurrent Local Delivery of Diflunisal Limits Bone Destruction but Fails To Improve Systemic Vancomycin Efficacy during Staphylococcus aureus Osteomyelitis. Antimicrob Agents Chemother [electronic-print]. 2020 Jun 6/23/2020; 64(7): PMID: 32340992, PMCID: PMC7318050, PII: AAC.00182-20, DOI: 10.1128/AAC.00182-20, ISSN: 1098-6596.
Urish KL, Cassat JE. Staphylococcus aureus Osteomyelitis: Bone, Bugs, and Surgery. Infect Immun [electronic-print]. 2020 Jun 6/22/2020; 88(7): PMID: 32094258, PMCID: PMC7309607, PII: IAI.00932-19, DOI: 10.1128/IAI.00932-19, ISSN: 1098-5522.
Potter AD, Butrico CE, Ford CA, Curry JM, Trenary IA, Tummarakota SS, Hendrix AS, Young JD, Cassat JE. Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection. Proc Natl Acad Sci U S A [print-electronic]. 2020 Jun 6/2/2020; 117(22): 12394-401. PMID: 32414924, PMCID: PMC7275739, PII: 1922211117, DOI: 10.1073/pnas.1922211117, ISSN: 1091-6490.
Peek CT, Ibberson CB, Cassat JE. Identification of Virulence Determinants During Host-Pathogen Interaction Using Tn-Seq Technology. Methods Mol Biol. 2020; 2069: 155-75. PMID: 31523773, DOI: 10.1007/978-1-4939-9849-4_12, ISSN: 1940-6029.
Ford CA, Hurford IM, Cassat JE. Antivirulence Strategies for the Treatment of Staphylococcus aureus Infections: A Mini Review. Front Microbiol. 2020; 11: 632706. PMID: 33519793, PMCID: PMC7840885, DOI: 10.3389/fmicb.2020.632706, ISSN: 1664-302X.
Perry WJ, Spraggins JM, Sheldon JR, Grunenwald CM, Heinrichs DE, Cassat JE, Skaar EP, Caprioli RM. Staphylococcus aureus exhibits heterogeneous siderophore production within the vertebrate host. Proc Natl Acad Sci U S A [print-electronic]. 2019 Oct 10/29/2019; 116(44): 21980-2. PMID: 31611408, PMCID: PMC6825271, PII: 1913991116, DOI: 10.1073/pnas.1913991116, ISSN: 1091-6490.
Krauss JL, Roper PM, Ballard A, Shih CC, Fitzpatrick JAJ, Cassat JE, Ng PY, Pavlos NJ, Veis DJ. Staphylococcus aureus Infects Osteoclasts and Replicates Intracellularly. MBio. 2019 Oct 10/15/2019; 10(5): PMID: 31615966, PMCID: PMC6794488, PII: mBio.02447-19, DOI: 10.1128/mBio.02447-19, ISSN: 2150-7511.
Ryan DJ, Patterson NH, Putnam NE, Wilde AD, Weiss A, Perry WJ, Cassat JE, Skaar EP, Caprioli RM, Spraggins JM. MicroLESA: Integrating Autofluorescence Microscopy, In Situ Micro-Digestions, and Liquid Extraction Surface Analysis for High Spatial Resolution Targeted Proteomic Studies. Anal. Chem [print-electronic]. 2019 Jun 6/18/2019; 91(12): 7578-85. PMID: 31149808, DOI: 10.1021/acs.analchem.8b05889, ISSN: 1520-6882.
Putnam NE, Fulbright LE, Curry JM, Ford CA, Petronglo JR, Hendrix AS, Cassat JE. MyD88 and IL-1R signaling drive antibacterial immunity and osteoclast-driven bone loss during Staphylococcus aureus osteomyelitis. PLoS Pathog. 2019 Apr; 15(4): e1007744. PMID: 30978245, PMCID: PMC6481883, PII: PPATHOGENS-D-18-02431, DOI: 10.1371/journal.ppat.1007744, ISSN: 1553-7374.
Buenrostro D, Kwakwa KA, Putnam NE, Merkel AR, Johnson JR, Cassat JE, Sterling JA. Early TGF-ß inhibition in mice reduces the incidence of breast cancer induced bone disease in a myeloid dependent manner. Bone [print-electronic]. 2018 Aug; 113: 77-88. PMID: 29753718, PMCID: PMC6118216, PII: S8756-3282(18)30187-X, DOI: 10.1016/j.bone.2018.05.008, ISSN: 1873-2763.
Buenrostro D, Kwakwa KA, Putnam NE, Merkel AR, Johnson JR, Cassat JE, Sterling JA. Early TGF-ß inhibition in mice reduces the incidence of breast cancer induced bone disease in a myeloid dependent manner. Bone [print-electronic]. 2018 Aug; 113: 77-88. PMID: 29753718, PMCID: PMC6118216, PII: S8756-3282(18)30187-X, DOI: 10.1016/j.bone.2018.05.008, ISSN: 1873-2763.
Brandt SL, Putnam NE, Cassat JE, Serezani CH. Innate Immunity to Staphylococcus aureus: Evolving Paradigms in Soft Tissue and Invasive Infections. J. Immunol. 2018 Jun 6/15/2018; 200(12): 3871-80. PMID: 29866769, PMCID: PMC6028009, PII: 200/12/3871, DOI: 10.4049/jimmunol.1701574, ISSN: 1550-6606.
Brandt SL, Putnam NE, Cassat JE, Serezani CH. Innate Immunity to Staphylococcus aureus: Evolving Paradigms in Soft Tissue and Invasive Infections. J. Immunol. 2018 Jun 6/15/2018; 200(12): 3871-80. PMID: 29866769, PMCID: PMC6028009, PII: 200/12/3871, DOI: 10.4049/jimmunol.1701574, ISSN: 1550-6606.
Cassat JE, Moore JL, Wilson KJ, Stark Z, Prentice BM, Van de Plas R, Perry WJ, Zhang Y, Virostko J, Colvin DC, Rose KL, Judd AM, Reyzer ML, Spraggins JM, Grunenwald CM, Gore JC, Caprioli RM, Skaar EP. Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions. Sci Transl Med. 2018 Mar 3/14/2018; 10(432): PMID: 29540616, PMCID: PMC6005374, PII: 10/432/eaan6361, DOI: 10.1126/scitranslmed.aan6361, ISSN: 1946-6242.
Cassat JE, Moore JL, Wilson KJ, Stark Z, Prentice BM, Van de Plas R, Perry WJ, Zhang Y, Virostko J, Colvin DC, Rose KL, Judd AM, Reyzer ML, Spraggins JM, Grunenwald CM, Gore JC, Caprioli RM, Skaar EP. Integrated molecular imaging reveals tissue heterogeneity driving host-pathogen interactions. Sci Transl Med. 2018 Mar 3/14/2018; 10(432): PMID: 29540616, PMCID: PMC6005374, PII: 10/432/eaan6361, DOI: 10.1126/scitranslmed.aan6361, ISSN: 1946-6242.
Frazier SB, Katz S, Wood JB, Cassat JE. An Unusual Source of Sepsis in Two Previously Healthy Children. Clin Pediatr (Phila) [print-electronic]. 2017 Oct 10/1/2017; 9922817738349. PMID: 29084446, DOI: 10.1177/0009922817738349, ISSN: 1938-2707.
Ford CA, Cassat JE. Advances in the local and targeted delivery of anti-infective agents for management of osteomyelitis. Expert Rev Anti Infect Ther [print-electronic]. 2017 Sep; 15(9): 851-60. PMID: 28837368, DOI: 10.1080/14787210.2017.1372192, ISSN: 1744-8336.
Thomsen IP, Sapparapu G, James DB, Cassat JE, Nagarsheth M, Kose N, Putnam N, Boguslawski KM, Jones LS, Wood JB, Creech CB, Torres VJ, Crowe JE. Monoclonal antibodies against the Staphylococcus aureus bicomponent leukotoxin AB (LukAB) isolated following invasive human infection reveal diverse binding and modes of action. J. Infect. Dis [print-electronic]. 2017 Feb 2/10/2017; PMID: 28186295, PII: 2982058, DOI: 10.1093/infdis/jix071, ISSN: 1537-6613.
Mendoza Bertelli A, Delpino MV, Lattar S, Giai C, Llana MN, Sanjuan N, Cassat JE, Sordelli D, Gómez MI. Staphylococcus aureus protein A enhances osteoclastogenesis via TNFR1 and EGFR signaling. Biochim. Biophys. Acta [print-electronic]. 2016 Oct; 1862(10): 1975-83. PMID: 27475257, PII: S0925-4439(16)30184-3, DOI: 10.1016/j.bbadis.2016.07.016, ISSN: 0006-3002.
Hendrix AS, Spoonmore TJ, Wilde AD, Putnam NE, Hammer ND, Snyder DJ, Guelcher SA, Skaar EP, Cassat JE. Repurposing the Nonsteroidal Anti-inflammatory Drug Diflunisal as an Osteoprotective, Antivirulence Therapy for Staphylococcus aureus Osteomyelitis. Antimicrob. Agents Chemother [electronic-print]. 2016 Sep; 60(9): 5322-30. PMID: 27324764, PMCID: PMC4997817, PII: AAC.00834-16, DOI: 10.1128/AAC.00834-16, ISSN: 1098-6596.
Loughran AJ, Gaddy D, Beenken KE, Meeker DG, Morello R, Zhao H, Byrum SD, Tackett AJ, Cassat JE, Smeltzer MS. Impact of sarA and Phenol-Soluble Modulins on the Pathogenesis of Osteomyelitis in Diverse Clinical Isolates of Staphylococcus aureus. Infect. Immun [electronic-print]. 2016 Sep; 84(9): 2586-94. PMID: 27354444, PMCID: PMC4995912, PII: IAI.00152-16, DOI: 10.1128/IAI.00152-16, ISSN: 1098-5522.
Wilde AD, Snyder DJ, Putnam NE, Valentino MD, Hammer ND, Lonergan ZR, Hinger SA, Aysanoa EE, Blanchard C, Dunman PM, Wasserman GA, Chen J, Shopsin B, Gilmore MS, Skaar EP, Cassat JE. Bacterial Hypoxic Responses Revealed as Critical Determinants of the Host-Pathogen Outcome by TnSeq Analysis of Staphylococcus aureus Invasive Infection. PLoS Pathog. 2015 Dec; 11(12): e1005341. PMID: 26684646, PMCID: PMC4684308, PII: PPATHOGENS-D-15-02340, DOI: 10.1371/journal.ppat.1005341, ISSN: 1553-7374.
Hammer ND, Cassat JE, Noto MJ, Lojek LJ, Chadha AD, Schmitz JE, Creech CB, Skaar EP. Inter- and intraspecies metabolite exchange promotes virulence of antibiotic-resistant Staphylococcus aureus. Cell Host Microbe. 2014 Oct 10/8/2014; 16(4): 531-7. PMID: 25299336, PMCID: PMC4197139, PII: S1931-3128(14)00335-7, DOI: 10.1016/j.chom.2014.09.002, ISSN: 1934-6069.
Gillon JE, Cassat JE, Di Pentima MC. Validation of two vancomycin nomograms in patients 10 years of age and older. J Clin Pharmacol [print-electronic]. 2014 Jan; 54(1): 35-8. PMID: 24249098, DOI: 10.1002/jcph.173, ISSN: 1552-4604.
Cassat JE, Smeltzer MS, Lee CY. Investigation of biofilm formation in clinical isolates of Staphylococcus aureus. Methods Mol. Biol. 2014; 1085: 195-211. PMID: 24085698, DOI: 10.1007/978-1-62703-664-1_12, ISSN: 1940-6029.
Cassat JE, Skaar EP. Recent advances in experimental models of osteomyelitis [editorial]. Expert Rev Anti Infect Ther. 2013 Dec; 11(12): 1263-5. PMID: 24215241, DOI: 10.1586/14787210.2013.858600, ISSN: 1744-8336.
Attia AS, Cassat JE, Aranmolate SO, Zimmerman LJ, Boyd KL, Skaar EP. Analysis of the Staphylococcus aureus abscess proteome identifies antimicrobial host proteins and bacterial stress responses at the host-pathogen interface. Pathog Dis [print-electronic]. 2013 Jul 7/11/2013; PMID: 23847107, PMCID: PMC3877740, DOI: 10.1111/2049-632X.12063, ISSN: 2049-632X.
Cassat JE, Hammer ND, Campbell JP, Benson MA, Perrien DS, Mrak LN, Smeltzer MS, Torres VJ, Skaar EP. A secreted bacterial protease tailors the Staphylococcus aureus virulence repertoire to modulate bone remodeling during osteomyelitis. Cell Host Microbe. 2013 Jun 6/12/2013; 13(6): 759-72. PMID: 23768499, PMCID: PMC3721972, PII: S1931-3128(13)00185-6, DOI: 10.1016/j.chom.2013.05.003, ISSN: 1934-6069.
Cassat JE, Skaar EP. Iron in infection and immunity. Cell Host Microbe. 2013 May 5/15/2013; 13(5): 509-19. PMID: 23684303, PMCID: PMC3676888, PII: S1931-3128(13)00152-2, DOI: 10.1016/j.chom.2013.04.010, ISSN: 1934-6069.
Hammer ND, Reniere ML, Cassat JE, Zhang Y, Hirsch AO, Indriati Hood M, Skaar EP. Two heme-dependent terminal oxidases power Staphylococcus aureus organ-specific colonization of the vertebrate host. MBio. 2013; 4(4): PMID: 23900169, PMCID: PMC3735196, PII: mBio.00241-13, DOI: 10.1128/mBio.00241-13, ISSN: 2150-7511.
Cassat JE, Skaar EP. Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity. Semin Immunopathol [print-electronic]. 2012 Mar; 34(2): 215-35. PMID: 22048835, PMCID: PMC3796439, DOI: 10.1007/s00281-011-0294-4, ISSN: 1863-2300.
Stenz L, François P, Fischer A, Huyghe A, Tangomo M, Hernandez D, Cassat J, Linder P, Schrenzel J. Impact of oleic acid (cis-9-octadecenoic acid) on bacterial viability and biofilm production in Staphylococcus aureus. FEMS Microbiol. Lett [print-electronic]. 2008 Oct; 287(2): 149-55. PMID: 18754790, PII: FML1316, DOI: 10.1111/j.1574-6968.2008.01316.x, ISSN: 0378-1097.
Tsang LH, Cassat JE, Shaw LN, Beenken KE, Smeltzer MS. Factors contributing to the biofilm-deficient phenotype of Staphylococcus aureus sarA mutants. PLoS ONE [print-electronic]. 2008; 3(10): e3361. PMID: 18846215, PMCID: PMC2556392, DOI: 10.1371/journal.pone.0003361, ISSN: 1932-6203.
Rice KC, Mann EE, Endres JL, Weiss EC, Cassat JE, Smeltzer MS, Bayles KW. The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2007 May 5/8/2007; 104(19): 8113-8. PMID: 17452642, PMCID: PMC1876580, PII: 0610226104, DOI: 10.1073/pnas.0610226104, ISSN: 0027-8424.
Cassat JE, Lee CY, Smeltzer MS. Investigation of biofilm formation in clinical isolates of Staphylococcus aureus. Methods Mol. Biol. 2007; 391: 127-44. PMID: 18025674, PMCID: PMC4098860, PII: 1-59745-468-0:127, DOI: 10.1007/978-1-59745-468-1_10, ISSN: 1064-3745.
Cassat J, Dunman PM, Murphy E, Projan SJ, Beenken KE, Palm KJ, Yang SJ, Rice KC, Bayles KW, Smeltzer MS. Transcriptional profiling of a Staphylococcus aureus clinical isolate and its isogenic agr and sarA mutants reveals global differences in comparison to the laboratory strain RN6390. Microbiology (Reading, Engl.). 2006 Oct; 152(Pt 10): 3075-90. PMID: 17005987, PII: 152/10/3075, DOI: 10.1099/mic.0.29033-0, ISSN: 1350-0872.
Cassat JE, Dunman PM, McAleese F, Murphy E, Projan SJ, Smeltzer MS. Comparative genomics of Staphylococcus aureus musculoskeletal isolates. J. Bacteriol. 2005 Jan; 187(2): 576-92. PMID: 15629929, PMCID: PMC543526, PII: 187/2/576, DOI: 10.1128/JB.187.2.576-592.2005, ISSN: 0021-9193.
Kaiser JR, Cassat JE, Lewno MJ. Should antibiotics be discontinued at 48 hours for negative late-onset sepsis evaluations in the neonatal intensive care unit?. J Perinatol. 2002 Sep; 22(6): 445-7. PMID: 12168120, DOI: 10.1038/sj.jp.7210764, ISSN: 0743-8346.