Faculty Appointments
Associate Professor of Cell & Developmental Biology
Education
Ph.D., Molecular, Cellular, and Developmental Biology , Yale University, New Haven, ConnecticutB.S., Biology, University of Georgia, Athens, Georgia
Office Address
Department of Cell and Developmental Biology
MCN Room T2209
Nashville, TN 37032
MCN Room T2209
Nashville, TN 37032
Research Description
Cell biological studies are limited by available technologies. For much of history, genomic and/or proteomic techniques were either completely unavailable or accessible by only a few experts, and microscopy techniques could only image one cell at a time. Today, modern microscopes have long surpassed their predecessors, capable of imaging tens to thousands of individual cells at once. In addition, many research centers now also have genomic and/or proteomic cores capable of analyzing large data sets. We and others are focusing these readily available technologies on re-examining a classic question: how does force-generating machinery assemble and function within a cell?
Our lab focuses on the first molecular motor that was discovered, myosin II. The classical function of myosin II is to generate “contractile” forces, generated by myosin heavy chains pulling on actin filaments. However, it is now known that myosin-II based contraction drives a plethora of other important cellular processes, including cell division, nuclear positioning, membrane bleb formation and retraction, 3D cell migration, tissue morphogenesis, mitochondrial fission, focal adhesion maturation, stem cell differentiation, retrograde flow of actin filaments, cell shape changes and the metastasis of solid tumors. Not surprisingly, there are multiple myosin II paralogs (i.e., heavy chains produced by different genes) that are used to accomplish these tasks. We started the lab by investigating two widely expressed myosin II paralogs, myosin IIA and myosin IIB. These paralogs are often referred to as “non-muscle”, but are expressed in both muscle and non-muscle cell types. Notably, our data suggest that myosin IIA and myosin IIB work in tandem to tune contractility within a cell. In our hands, this is particularly clear during mitosis and cytokinesis in non-muscle cells. Our data also suggest that IIA/IIB are vital for forming a muscle sarcomere, the contractile unit within cardiomyocytes which drives heart muscle contraction. These findings have prompted us to take a closer look at similarities and differences between the assembly of myosin II-based contractile systems in muscle versus non-muscle cells. For this work, we created an assay using human cardiomyocytes to monitor sarcomere assembly, finding that a population of muscle stress fibers (which appear similar to actin arcs in non-muscle cells) are essential sarcomere precursors. We are now using this assay to explore the mechanisms underlying stress fiber assembly, as well as the transition of these stress fibers into the sarcomere-containing “myofibrils” that drive the heartbeat.
Our lab focuses on the first molecular motor that was discovered, myosin II. The classical function of myosin II is to generate “contractile” forces, generated by myosin heavy chains pulling on actin filaments. However, it is now known that myosin-II based contraction drives a plethora of other important cellular processes, including cell division, nuclear positioning, membrane bleb formation and retraction, 3D cell migration, tissue morphogenesis, mitochondrial fission, focal adhesion maturation, stem cell differentiation, retrograde flow of actin filaments, cell shape changes and the metastasis of solid tumors. Not surprisingly, there are multiple myosin II paralogs (i.e., heavy chains produced by different genes) that are used to accomplish these tasks. We started the lab by investigating two widely expressed myosin II paralogs, myosin IIA and myosin IIB. These paralogs are often referred to as “non-muscle”, but are expressed in both muscle and non-muscle cell types. Notably, our data suggest that myosin IIA and myosin IIB work in tandem to tune contractility within a cell. In our hands, this is particularly clear during mitosis and cytokinesis in non-muscle cells. Our data also suggest that IIA/IIB are vital for forming a muscle sarcomere, the contractile unit within cardiomyocytes which drives heart muscle contraction. These findings have prompted us to take a closer look at similarities and differences between the assembly of myosin II-based contractile systems in muscle versus non-muscle cells. For this work, we created an assay using human cardiomyocytes to monitor sarcomere assembly, finding that a population of muscle stress fibers (which appear similar to actin arcs in non-muscle cells) are essential sarcomere precursors. We are now using this assay to explore the mechanisms underlying stress fiber assembly, as well as the transition of these stress fibers into the sarcomere-containing “myofibrils” that drive the heartbeat.
Research Keywords
Cell physiology, Cardiovascular science, Super-resolution microscopy, Cell motility, Cardiomyocyte proliferation
Publications
Taneja N, Burnette DT. Myosin IIA drives membrane bleb retraction. Mol. Biol. Cell [print-electronic]. 2019 Apr 4/15/2019; 30(9): 1051-9. PMID: 30785846, PMCID: PMC6724514, DOI: 10.1091/mbc.E18-11-0752, ISSN: 1939-4586.
Howard AM, LaFever KS, Fenix AM, Scurrah CR, Lau KS, Burnette DT, Bhave G, Ferrell N, Page-McCaw A. DSS-induced damage to basement membranes is repaired by matrix replacement and crosslinking. J. Cell. Sci. 2019 Apr 4/8/2019; 132(7): PMID: 30837285, PMCID: PMC6467488, PII: jcs.226860, DOI: 10.1242/jcs.226860, ISSN: 1477-9137.
Jeppesen DK, Fenix AM, Franklin JL, Higginbotham JN, Zhang Q, Zimmerman LJ, Liebler DC, Ping J, Liu Q, Evans R, Fissell WH, Patton JG, Rome LH, Burnette DT, Coffey RJ. Reassessment of Exosome Composition. Cell. 2019 Apr 4/4/2019; 177(2): 428-445.e18. PMID: 30951670, PMCID: PMC6664447, PII: S0092-8674(19)30212-0, DOI: 10.1016/j.cell.2019.02.029, ISSN: 1097-4172.
Taneja N, Rathbun L, Hehnly H, Burnette DT. The balance between adhesion and contraction during cell division. Curr. Opin. Cell Biol [print-electronic]. 2019 Feb; 56: 45-52. PMID: 30268802, PMCID: PMC6363874, PII: S0955-0674(18)30095-4, DOI: 10.1016/j.ceb.2018.09.001, ISSN: 1879-0410.
Jiu Y, Kumari R, Fenix AM, Schaible N, Liu X, Varjosalo M, Krishnan R, Burnette DT, Lappalainen P. Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles. Curr. Biol [print-electronic]. 2019 Jan 1/7/2019; 29(1): 81-92.e5. PMID: 30581023, PMCID: PMC6347125, PII: S0960-9822(18)31544-6, DOI: 10.1016/j.cub.2018.11.045, ISSN: 1879-0445.
Fenix AM, Neininger AC, Taneja N, Hyde K, Visetsouk MR, Garde RJ, Liu B, Nixon BR, Manalo AE, Becker JR, Crawley SW, Bader DM, Tyska MJ, Liu Q, Gutzman JH, Burnette DT. Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes. Elife. 2018 Dec 12/12/2018; 7: PMID: 30540249, PMCID: PMC6307863, PII: 42144, DOI: 10.7554/eLife.42144, ISSN: 2050-084X.
Fenix AM, Burnette DT. Assembly of myosin II filament arrays: Network Contraction versus Expansion. Cytoskeleton (Hoboken) [print-electronic]. 2018 Dec; 75(12): 545-9. PMID: 30126071, PMCID: PMC6368487, DOI: 10.1002/cm.21487, ISSN: 1949-3592.
Pathak A, Stanley EM, Hickman FE, Wallace N, Brewer B, Li D, Gluska S, Perlson E, Fuhrmann S, Akassoglou K, Bronfman F, Casaccia P, Burnette DT, Carter BD. Retrograde Degenerative Signaling Mediated by the p75 Neurotrophin Receptor Requires p150Glued Deacetylation by Axonal HDAC1. Dev. Cell. 2018 Aug 8/6/2018; 46(3): 376-387.e7. PMID: 30086304, PMCID: PMC6093198, PII: S1534-5807(18)30554-9, DOI: 10.1016/j.devcel.2018.07.001, ISSN: 1878-1551.
Manalo A, Schroer AK, Fenix AM, Shancer Z, Coogan J, Brolsma T, Burnette DT, Merryman WD, Bader DM. Loss of CENP-F Results in Dilated Cardiomyopathy with Severe Disruption of Cardiac Myocyte Architecture. Sci Rep. 2018 May 5/15/2018; 8(1): 7546. PMID: 29765066, PMCID: PMC5953941, PII: 10.1038/s41598-018-25774-1, DOI: 10.1038/s41598-018-25774-1, ISSN: 2045-2322.
Lehtimäki JI, Fenix AM, Kotila TM, Balistreri G, Paavolainen L, Varjosalo M, Burnette DT, Lappalainen P. UNC-45a promotes myosin folding and stress fiber assembly. J. Cell Biol [print-electronic]. 2017 Dec 12/4/2017; 216(12): 4053-72. PMID: 29055011, PMCID: PMC5716280, PII: jcb.201703107, DOI: 10.1083/jcb.201703107, ISSN: 1540-8140.
Guo M, Pegoraro AF, Mao A, Zhou EH, Arany PR, Han Y, Burnette DT, Jensen MH, Kasza KE, Moore JR, Mackintosh FC, Fredberg JJ, Mooney DJ, Lippincott-Schwartz J, Weitz DA. Cell volume change through water efflux impacts cell stiffness and stem cell fate. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2017 Oct 10/10/2017; 114(41): E8618-E8627. PMID: 28973866, PMCID: PMC5642688, PII: 1705179114, DOI: 10.1073/pnas.1705179114, ISSN: 1091-6490.
Balikov DA, Crowder SW, Boire TC, Lee JB, Gupta MK, Fenix AM, Lewis HN, Ambrose CM, Short PA, Kim CS, Burnette DT, Reilly MA, Murthy NS, Kang ML, Kim WS, Sung HJ. Tunable Surface Repellency Maintains Stemness and Redox Capacity of Human Mesenchymal Stem Cells. ACS Appl Mater Interfaces [print-electronic]. 2017 Jul 7/12/2017; 9(27): 22994-3006. PMID: 28621931, PMCID: PMC5687519, DOI: 10.1021/acsami.7b06103, ISSN: 1944-8252.
Taneja N, Fenix AM, Rathbun L, Millis BA, Tyska MJ, Hehnly H, Burnette DT. Focal adhesions control cleavage furrow shape and spindle tilt during mitosis. Sci Rep. 2016 Jul 7/19/2016; 6: 29846. PMID: 27432211, PMCID: PMC4949487, PII: srep29846, DOI: 10.1038/srep29846, ISSN: 2045-2322.
Fenix AM, Taneja N, Buttler CA, Lewis J, Van Engelenburg SB, Ohi R, Burnette DT. Expansion and concatenation of non-muscle myosin IIA filaments drive cellular contractile system formation during interphase and mitosis. Mol. Biol. Cell [print-electronic]. 2016 Mar 3/9/2016; PMID: 26960797, PMCID: PMC4850034, PII: mbc.E15-10-0725, DOI: 10.1091/mbc.E15-10-0725, ISSN: 1939-4586.
Fenix AM, Burnette DT. A small part of myosin IIB takes on a big role in cell polarity. J. Cell Biol. 2015 Apr 4/13/2015; 209(1): 11-2. PMID: 25869662, PMCID: PMC4395479, PII: jcb.201503079, DOI: 10.1083/jcb.201503079, ISSN: 1540-8140.
Battle C, Ott CM, Burnette DT, Lippincott-Schwartz J, Schmidt CF. Intracellular and extracellular forces drive primary cilia movement. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Feb 2/3/2015; 112(5): 1410-5. PMID: 25605896, PMCID: PMC4321243, PII: 1421845112, DOI: 10.1073/pnas.1421845112, ISSN: 1091-6490.
Burnette DT, Shao L, Ott C, Pasapera AM, Fischer RS, Baird MA, Der Loughian C, Delanoe-Ayari H, Paszek MJ, Davidson MW, Betzig E, Lippincott-Schwartz J. A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells. J. Cell Biol [print-electronic]. 2014 Apr 4/14/2014; 205(1): 83-96. PMID: 24711500, PMCID: PMC3987145, PII: jcb.201311104, DOI: 10.1083/jcb.201311104, ISSN: 1540-8140.
Millis BA, Burnette DT, Lippincott-Schwartz J, Kachar B. Superresolution imaging with standard fluorescent probes. Curr Protoc Cell Biol. 2013; 60: Unit 21.8.. PMID: 24510788, PMCID: PMC3970242, DOI: 10.1002/0471143030.cb2108s60, ISSN: 1934-2616.
Cox S, Rosten E, Monypenny J, Jovanovic-Talisman T, Burnette DT, Lippincott-Schwartz J, Jones GE, Heintzmann R. Bayesian localization microscopy reveals nanoscale podosome dynamics. Nat. Methods. 2012 Feb; 9(2): 195-200. PMID: 22138825, PMCID: PMC3272474, PII: nmeth.1812, DOI: 10.1038/nmeth.1812, ISSN: 1548-7105.
Burnette DT, Sengupta P, Dai Y, Lippincott-Schwartz J, Kachar B. Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2011 Dec 12/27/2011; 108(52): 21081-6. PMID: 22167805, PMCID: PMC3248526, PII: 1117430109, DOI: 10.1073/pnas.1117430109, ISSN: 1091-6490.
Burnette DT, Manley S, Sengupta P, Sougrat R, Davidson MW, Kachar B, Lippincott-Schwartz J. A role for actin arcs in the leading-edge advance of migrating cells. Nat. Cell Biol [print-electronic]. 2011 Apr; 13(4): 371-81. PMID: 21423177, PMCID: PMC3646481, PII: ncb2205, DOI: 10.1038/ncb2205, ISSN: 1476-4679.
Burnette DT, Ji L, Schaefer AW, Medeiros NA, Danuser G, Forscher P. Myosin II activity facilitates microtubule bundling in the neuronal growth cone neck. Dev. Cell. 2008 Jul; 15(1): 163-9. PMID: 18606149, PMCID: PMC2548298, PII: S1534-5807(08)00241-4, DOI: 10.1016/j.devcel.2008.05.016, ISSN: 1878-1551.
Burnette DT, Schaefer AW, Ji L, Danuser G, Forscher P. Filopodial actin bundles are not necessary for microtubule advance into the peripheral domain of Aplysia neuronal growth cones. Nat. Cell Biol [print-electronic]. 2007 Dec; 9(12): 1360-9. PMID: 18026092, PII: ncb1655, DOI: 10.1038/ncb1655, ISSN: 1465-7392.
Medeiros NA, Burnette DT, Forscher P. Myosin II functions in actin-bundle turnover in neuronal growth cones. Nat. Cell Biol [print-electronic]. 2006 Mar; 8(3): 215-26. PMID: 16501565, PII: ncb1367, DOI: 10.1038/ncb1367, ISSN: 1465-7392.
Qin H, Burnette DT, Bae YK, Forscher P, Barr MM, Rosenbaum JL. Intraflagellar transport is required for the vectorial movement of TRPV channels in the ciliary membrane. Curr. Biol. 2005 Sep 9/20/2005; 15(18): 1695-9. PMID: 16169494, PII: S0960-9822(05)00966-8, DOI: 10.1016/j.cub.2005.08.047, ISSN: 0960-9822.
Redeker V, Levilliers N, Vinolo E, Rossier J, Jaillard D, Burnette D, Gaertig J, Bré MH. Mutations of tubulin glycylation sites reveal cross-talk between the C termini of alpha- and beta-tubulin and affect the ciliary matrix in Tetrahymena. J. Biol. Chem [print-electronic]. 2005 Jan 1/7/2005; 280(1): 596-606. PMID: 15492004, PII: M408324200, DOI: 10.1074/jbc.M408324200, ISSN: 0021-9258.
Matroule JY, Lam H, Burnette DT, Jacobs-Wagner C. Cytokinesis monitoring during development; rapid pole-to-pole shuttling of a signaling protein by localized kinase and phosphatase in Caulobacter. Cell. 2004 Sep 9/3/2004; 118(5): 579-90. PMID: 15339663, PII: S0092867404007950, DOI: 10.1016/j.cell.2004.08.019, ISSN: 0092-8674.
Zhang XF, Schaefer AW, Burnette DT, Schoonderwoert VT, Forscher P. Rho-dependent contractile responses in the neuronal growth cone are independent of classical peripheral retrograde actin flow. Neuron. 2003 Dec 12/4/2003; 40(5): 931-44. PMID: 14659092, PII: S0896627303007542, ISSN: 0896-6273.
Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bré MH, Levilliers N, Gorovsky MA, Gaertig J. Polyglycylation of tubulin is essential and affects cell motility and division in Tetrahymena thermophila. J. Cell Biol. 2000 May 5/29/2000; 149(5): 1097-106. PMID: 10831613, PMCID: PMC2174830, ISSN: 0021-9525.
Howard AM, LaFever KS, Fenix AM, Scurrah CR, Lau KS, Burnette DT, Bhave G, Ferrell N, Page-McCaw A. DSS-induced damage to basement membranes is repaired by matrix replacement and crosslinking. J. Cell. Sci. 2019 Apr 4/8/2019; 132(7): PMID: 30837285, PMCID: PMC6467488, PII: jcs.226860, DOI: 10.1242/jcs.226860, ISSN: 1477-9137.
Jeppesen DK, Fenix AM, Franklin JL, Higginbotham JN, Zhang Q, Zimmerman LJ, Liebler DC, Ping J, Liu Q, Evans R, Fissell WH, Patton JG, Rome LH, Burnette DT, Coffey RJ. Reassessment of Exosome Composition. Cell. 2019 Apr 4/4/2019; 177(2): 428-445.e18. PMID: 30951670, PMCID: PMC6664447, PII: S0092-8674(19)30212-0, DOI: 10.1016/j.cell.2019.02.029, ISSN: 1097-4172.
Taneja N, Rathbun L, Hehnly H, Burnette DT. The balance between adhesion and contraction during cell division. Curr. Opin. Cell Biol [print-electronic]. 2019 Feb; 56: 45-52. PMID: 30268802, PMCID: PMC6363874, PII: S0955-0674(18)30095-4, DOI: 10.1016/j.ceb.2018.09.001, ISSN: 1879-0410.
Jiu Y, Kumari R, Fenix AM, Schaible N, Liu X, Varjosalo M, Krishnan R, Burnette DT, Lappalainen P. Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles. Curr. Biol [print-electronic]. 2019 Jan 1/7/2019; 29(1): 81-92.e5. PMID: 30581023, PMCID: PMC6347125, PII: S0960-9822(18)31544-6, DOI: 10.1016/j.cub.2018.11.045, ISSN: 1879-0445.
Fenix AM, Neininger AC, Taneja N, Hyde K, Visetsouk MR, Garde RJ, Liu B, Nixon BR, Manalo AE, Becker JR, Crawley SW, Bader DM, Tyska MJ, Liu Q, Gutzman JH, Burnette DT. Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes. Elife. 2018 Dec 12/12/2018; 7: PMID: 30540249, PMCID: PMC6307863, PII: 42144, DOI: 10.7554/eLife.42144, ISSN: 2050-084X.
Fenix AM, Burnette DT. Assembly of myosin II filament arrays: Network Contraction versus Expansion. Cytoskeleton (Hoboken) [print-electronic]. 2018 Dec; 75(12): 545-9. PMID: 30126071, PMCID: PMC6368487, DOI: 10.1002/cm.21487, ISSN: 1949-3592.
Pathak A, Stanley EM, Hickman FE, Wallace N, Brewer B, Li D, Gluska S, Perlson E, Fuhrmann S, Akassoglou K, Bronfman F, Casaccia P, Burnette DT, Carter BD. Retrograde Degenerative Signaling Mediated by the p75 Neurotrophin Receptor Requires p150Glued Deacetylation by Axonal HDAC1. Dev. Cell. 2018 Aug 8/6/2018; 46(3): 376-387.e7. PMID: 30086304, PMCID: PMC6093198, PII: S1534-5807(18)30554-9, DOI: 10.1016/j.devcel.2018.07.001, ISSN: 1878-1551.
Manalo A, Schroer AK, Fenix AM, Shancer Z, Coogan J, Brolsma T, Burnette DT, Merryman WD, Bader DM. Loss of CENP-F Results in Dilated Cardiomyopathy with Severe Disruption of Cardiac Myocyte Architecture. Sci Rep. 2018 May 5/15/2018; 8(1): 7546. PMID: 29765066, PMCID: PMC5953941, PII: 10.1038/s41598-018-25774-1, DOI: 10.1038/s41598-018-25774-1, ISSN: 2045-2322.
Lehtimäki JI, Fenix AM, Kotila TM, Balistreri G, Paavolainen L, Varjosalo M, Burnette DT, Lappalainen P. UNC-45a promotes myosin folding and stress fiber assembly. J. Cell Biol [print-electronic]. 2017 Dec 12/4/2017; 216(12): 4053-72. PMID: 29055011, PMCID: PMC5716280, PII: jcb.201703107, DOI: 10.1083/jcb.201703107, ISSN: 1540-8140.
Guo M, Pegoraro AF, Mao A, Zhou EH, Arany PR, Han Y, Burnette DT, Jensen MH, Kasza KE, Moore JR, Mackintosh FC, Fredberg JJ, Mooney DJ, Lippincott-Schwartz J, Weitz DA. Cell volume change through water efflux impacts cell stiffness and stem cell fate. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2017 Oct 10/10/2017; 114(41): E8618-E8627. PMID: 28973866, PMCID: PMC5642688, PII: 1705179114, DOI: 10.1073/pnas.1705179114, ISSN: 1091-6490.
Balikov DA, Crowder SW, Boire TC, Lee JB, Gupta MK, Fenix AM, Lewis HN, Ambrose CM, Short PA, Kim CS, Burnette DT, Reilly MA, Murthy NS, Kang ML, Kim WS, Sung HJ. Tunable Surface Repellency Maintains Stemness and Redox Capacity of Human Mesenchymal Stem Cells. ACS Appl Mater Interfaces [print-electronic]. 2017 Jul 7/12/2017; 9(27): 22994-3006. PMID: 28621931, PMCID: PMC5687519, DOI: 10.1021/acsami.7b06103, ISSN: 1944-8252.
Taneja N, Fenix AM, Rathbun L, Millis BA, Tyska MJ, Hehnly H, Burnette DT. Focal adhesions control cleavage furrow shape and spindle tilt during mitosis. Sci Rep. 2016 Jul 7/19/2016; 6: 29846. PMID: 27432211, PMCID: PMC4949487, PII: srep29846, DOI: 10.1038/srep29846, ISSN: 2045-2322.
Fenix AM, Taneja N, Buttler CA, Lewis J, Van Engelenburg SB, Ohi R, Burnette DT. Expansion and concatenation of non-muscle myosin IIA filaments drive cellular contractile system formation during interphase and mitosis. Mol. Biol. Cell [print-electronic]. 2016 Mar 3/9/2016; PMID: 26960797, PMCID: PMC4850034, PII: mbc.E15-10-0725, DOI: 10.1091/mbc.E15-10-0725, ISSN: 1939-4586.
Fenix AM, Burnette DT. A small part of myosin IIB takes on a big role in cell polarity. J. Cell Biol. 2015 Apr 4/13/2015; 209(1): 11-2. PMID: 25869662, PMCID: PMC4395479, PII: jcb.201503079, DOI: 10.1083/jcb.201503079, ISSN: 1540-8140.
Battle C, Ott CM, Burnette DT, Lippincott-Schwartz J, Schmidt CF. Intracellular and extracellular forces drive primary cilia movement. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2015 Feb 2/3/2015; 112(5): 1410-5. PMID: 25605896, PMCID: PMC4321243, PII: 1421845112, DOI: 10.1073/pnas.1421845112, ISSN: 1091-6490.
Burnette DT, Shao L, Ott C, Pasapera AM, Fischer RS, Baird MA, Der Loughian C, Delanoe-Ayari H, Paszek MJ, Davidson MW, Betzig E, Lippincott-Schwartz J. A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells. J. Cell Biol [print-electronic]. 2014 Apr 4/14/2014; 205(1): 83-96. PMID: 24711500, PMCID: PMC3987145, PII: jcb.201311104, DOI: 10.1083/jcb.201311104, ISSN: 1540-8140.
Millis BA, Burnette DT, Lippincott-Schwartz J, Kachar B. Superresolution imaging with standard fluorescent probes. Curr Protoc Cell Biol. 2013; 60: Unit 21.8.. PMID: 24510788, PMCID: PMC3970242, DOI: 10.1002/0471143030.cb2108s60, ISSN: 1934-2616.
Cox S, Rosten E, Monypenny J, Jovanovic-Talisman T, Burnette DT, Lippincott-Schwartz J, Jones GE, Heintzmann R. Bayesian localization microscopy reveals nanoscale podosome dynamics. Nat. Methods. 2012 Feb; 9(2): 195-200. PMID: 22138825, PMCID: PMC3272474, PII: nmeth.1812, DOI: 10.1038/nmeth.1812, ISSN: 1548-7105.
Burnette DT, Sengupta P, Dai Y, Lippincott-Schwartz J, Kachar B. Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2011 Dec 12/27/2011; 108(52): 21081-6. PMID: 22167805, PMCID: PMC3248526, PII: 1117430109, DOI: 10.1073/pnas.1117430109, ISSN: 1091-6490.
Burnette DT, Manley S, Sengupta P, Sougrat R, Davidson MW, Kachar B, Lippincott-Schwartz J. A role for actin arcs in the leading-edge advance of migrating cells. Nat. Cell Biol [print-electronic]. 2011 Apr; 13(4): 371-81. PMID: 21423177, PMCID: PMC3646481, PII: ncb2205, DOI: 10.1038/ncb2205, ISSN: 1476-4679.
Burnette DT, Ji L, Schaefer AW, Medeiros NA, Danuser G, Forscher P. Myosin II activity facilitates microtubule bundling in the neuronal growth cone neck. Dev. Cell. 2008 Jul; 15(1): 163-9. PMID: 18606149, PMCID: PMC2548298, PII: S1534-5807(08)00241-4, DOI: 10.1016/j.devcel.2008.05.016, ISSN: 1878-1551.
Burnette DT, Schaefer AW, Ji L, Danuser G, Forscher P. Filopodial actin bundles are not necessary for microtubule advance into the peripheral domain of Aplysia neuronal growth cones. Nat. Cell Biol [print-electronic]. 2007 Dec; 9(12): 1360-9. PMID: 18026092, PII: ncb1655, DOI: 10.1038/ncb1655, ISSN: 1465-7392.
Medeiros NA, Burnette DT, Forscher P. Myosin II functions in actin-bundle turnover in neuronal growth cones. Nat. Cell Biol [print-electronic]. 2006 Mar; 8(3): 215-26. PMID: 16501565, PII: ncb1367, DOI: 10.1038/ncb1367, ISSN: 1465-7392.
Qin H, Burnette DT, Bae YK, Forscher P, Barr MM, Rosenbaum JL. Intraflagellar transport is required for the vectorial movement of TRPV channels in the ciliary membrane. Curr. Biol. 2005 Sep 9/20/2005; 15(18): 1695-9. PMID: 16169494, PII: S0960-9822(05)00966-8, DOI: 10.1016/j.cub.2005.08.047, ISSN: 0960-9822.
Redeker V, Levilliers N, Vinolo E, Rossier J, Jaillard D, Burnette D, Gaertig J, Bré MH. Mutations of tubulin glycylation sites reveal cross-talk between the C termini of alpha- and beta-tubulin and affect the ciliary matrix in Tetrahymena. J. Biol. Chem [print-electronic]. 2005 Jan 1/7/2005; 280(1): 596-606. PMID: 15492004, PII: M408324200, DOI: 10.1074/jbc.M408324200, ISSN: 0021-9258.
Matroule JY, Lam H, Burnette DT, Jacobs-Wagner C. Cytokinesis monitoring during development; rapid pole-to-pole shuttling of a signaling protein by localized kinase and phosphatase in Caulobacter. Cell. 2004 Sep 9/3/2004; 118(5): 579-90. PMID: 15339663, PII: S0092867404007950, DOI: 10.1016/j.cell.2004.08.019, ISSN: 0092-8674.
Zhang XF, Schaefer AW, Burnette DT, Schoonderwoert VT, Forscher P. Rho-dependent contractile responses in the neuronal growth cone are independent of classical peripheral retrograde actin flow. Neuron. 2003 Dec 12/4/2003; 40(5): 931-44. PMID: 14659092, PII: S0896627303007542, ISSN: 0896-6273.
Xia L, Hai B, Gao Y, Burnette D, Thazhath R, Duan J, Bré MH, Levilliers N, Gorovsky MA, Gaertig J. Polyglycylation of tubulin is essential and affects cell motility and division in Tetrahymena thermophila. J. Cell Biol. 2000 May 5/29/2000; 149(5): 1097-106. PMID: 10831613, PMCID: PMC2174830, ISSN: 0021-9525.
Available Postdoctoral Position Details
Posted: 5/14/2014
A postdoctoral position ia available applying superresolution microscopy to study the formation of myosin II-filaments within motile cells.
A postdoctoral position ia available applying superresolution microscopy to study the formation of myosin II-filaments within motile cells.