Ashley Brady, Ph.D.

Assistant Professor

ashley.brady@vanderbilt.edu
Faculty Appointments
Assistant Professor of Medical Education & Administration (VU)
Education
Ph.D., Pharmacology , Vanderbilt University, Nashville, TennesseeB.A., Centre College, Danville, Kentucky
Publications
Brady AE, Chen Y, Limbird LE, Wang Q. Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs. Methods Mol. Biol. 2011; 746: 347-55. PMID: 21607867, DOI: 10.1007/978-1-61779-126-0_19, ISSN: 1940-6029.

Shirey JK, Brady AE, Jones PJ, Davis AA, Bridges TM, Kennedy JP, Jadhav SB, Menon UN, Xiang Z, Watson ML, Christian EP, Doherty JJ, Quirk MC, Snyder DH, Lah JJ, Levey AI, Nicolle MM, Lindsley CW, Conn PJ. A selective allosteric potentiator of the M1 muscarinic acetylcholine receptor increases activity of medial prefrontal cortical neurons and restores impairments in reversal learning. J. Neurosci. 2009 Nov 11/11/2009; 29(45): 14271-86. PMID: 19906975, PMCID: PMC2811323, PII: 29/45/14271, DOI: 10.1523/JNEUROSCI.3930-09.2009, ISSN: 1529-2401.

Kennedy JP, Bridges TM, Gentry PR, Brogan JT, Kane AS, Jones CK, Brady AE, Shirey JK, Conn PJ, Lindsley CW. Synthesis and structure-activity relationships of allosteric potentiators of the m(4) muscarinic acetylcholine receptor. ChemMedChem. 2009 Oct; 4(10): 1600-7. PMID: 19705385, PMCID: PMC2887613, DOI: 10.1002/cmdc.200900231, ISSN: 1860-7187.

Marlo JE, Niswender CM, Days EL, Bridges TM, Xiang Y, Rodriguez AL, Shirey JK, Brady AE, Nalywajko T, Luo Q, Austin CA, Williams MB, Kim K, Williams R, Orton D, Brown HA, Lindsley CW, Weaver CD, Conn PJ. Discovery and characterization of novel allosteric potentiators of M1 muscarinic receptors reveals multiple modes of activity. Mol. Pharmacol [print-electronic]. 2009 Mar; 75(3): 577-88. PMID: 19047481, PMCID: PMC2684909, PII: mol.108.052886, DOI: 10.1124/mol.108.052886, ISSN: 1521-0111.

Brady AE, Jones CK, Bridges TM, Kennedy JP, Thompson AD, Heiman JU, Breininger ML, Gentry PR, Yin H, Jadhav SB, Shirey JK, Conn PJ, Lindsley CW. Centrally active allosteric potentiators of the M4 muscarinic acetylcholine receptor reverse amphetamine-induced hyperlocomotor activity in rats. J. Pharmacol. Exp. Ther [print-electronic]. 2008 Dec; 327(3): 941-53. PMID: 18772318, PMCID: PMC2745822, PII: jpet.108.140350, DOI: 10.1124/jpet.108.140350, ISSN: 1521-0103.

Miller NR, Daniels RN, Bridges TM, Brady AE, Conn PJ, Lindsley CW. Synthesis and SAR of analogs of the M1 allosteric agonist TBPB. Part II: Amides, sulfonamides and ureas--the effect of capping the distal basic piperidine nitrogen. Bioorg. Med. Chem. Lett [print-electronic]. 2008 Oct 10/15/2008; 18(20): 5443-7. PMID: 18829311, PMCID: PMC3177607, PII: S0960-894X(08)01088-3, DOI: 10.1016/j.bmcl.2008.09.032, ISSN: 1464-3405.

Bridges TM, Brady AE, Kennedy JP, Daniels RN, Miller NR, Kim K, Breininger ML, Gentry PR, Brogan JT, Jones CK, Conn PJ, Lindsley CW. Synthesis and SAR of analogues of the M1 allosteric agonist TBPB. Part I: Exploration of alternative benzyl and privileged structure moieties. Bioorg. Med. Chem. Lett [print-electronic]. 2008 Oct 10/15/2008; 18(20): 5439-42. PMID: 18805692, PMCID: PMC3177598, PII: S0960-894X(08)01078-0, DOI: 10.1016/j.bmcl.2008.09.023, ISSN: 1464-3405.

Jones CK, Brady AE, Davis AA, Xiang Z, Bubser M, Tantawy MN, Kane AS, Bridges TM, Kennedy JP, Bradley SR, Peterson TE, Ansari MS, Baldwin RM, Kessler RM, Deutch AY, Lah JJ, Levey AI, Lindsley CW, Conn PJ. Novel selective allosteric activator of the M1 muscarinic acetylcholine receptor regulates amyloid processing and produces antipsychotic-like activity in rats. J. Neurosci. 2008 Oct 10/8/2008; 28(41): 10422-33. PMID: 18842902, PMCID: PMC2577155, PII: 28/41/10422, DOI: 10.1523/JNEUROSCI.1850-08.2008, ISSN: 1529-2401.

Shirey JK, Xiang Z, Orton D, Brady AE, Johnson KA, Williams R, Ayala JE, Rodriguez AL, Wess J, Weaver D, Niswender CM, Conn PJ. An allosteric potentiator of M4 mAChR modulates hippocampal synaptic transmission. Nat. Chem. Biol [print-electronic]. 2008 Jan; 4(1): 42-50. PMID: 18059262, PII: nchembio.2007.55, DOI: 10.1038/nchembio.2007.55, ISSN: 1552-4469.

Hemstapat K, Da Costa H, Nong Y, Brady AE, Luo Q, Niswender CM, Tamagnan GD, Conn PJ. A novel family of potent negative allosteric modulators of group II metabotropic glutamate receptors. J. Pharmacol. Exp. Ther [print-electronic]. 2007 Jul; 322(1): 254-64. PMID: 17416742, PII: jpet.106.117093, DOI: 10.1124/jpet.106.117093, ISSN: 0022-3565.

Hemstapat K, de Paulis T, Chen Y, Brady AE, Grover VK, Alagille D, Tamagnan GD, Conn PJ. A novel class of positive allosteric modulators of metabotropic glutamate receptor subtype 1 interact with a site distinct from that of negative allosteric modulators. Mol. Pharmacol [print-electronic]. 2006 Aug; 70(2): 616-26. PMID: 16645124, PII: mol.105.021857, DOI: 10.1124/mol.105.021857, ISSN: 0026-895X.

Brady AE, Wang Q, Allen PB, Rizzo M, Greengard P, Limbird LE. Alpha 2-adrenergic agonist enrichment of spinophilin at the cell surface involves beta gamma subunits of Gi proteins and is preferentially induced by the alpha 2A-subtype. Mol. Pharmacol [print-electronic]. 2005 May; 67(5): 1690-6. PMID: 15705742, PII: mol.104.005215, DOI: 10.1124/mol.104.005215, ISSN: 0026-895X.

Wang Q, Zhao J, Brady AE, Feng J, Allen PB, Lefkowitz RJ, Greengard P, Limbird LE. Spinophilin blocks arrestin actions in vitro and in vivo at G protein-coupled receptors. Science. 2004 Jun 6/25/2004; 304(5679): 1940-4. PMID: 15218143, PII: 304/5679/1940, DOI: 10.1126/science.1098274, ISSN: 1095-9203.

Brady AE, Wang Q, Limbird LE. Study of G-protein-coupled receptor-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs. Methods Mol. Biol. 2004; 259: 371-8. PMID: 15250505, DOI: 10.1385/1-59259-754-8:371, ISSN: 1064-3745.

Tan CM, Brady AE, Nickols HH, Wang Q, Limbird LE. Membrane trafficking of G protein-coupled receptors. Annu. Rev. Pharmacol. Toxicol. 2004; 44: 559-609. PMID: 14744258, DOI: 10.1146/annurev.pharmtox.44.101802.121558, ISSN: 0362-1642.

Brady AE, Wang Q, Colbran RJ, Allen PB, Greengard P, Limbird LE. Spinophilin stabilizes cell surface expression of alpha 2B-adrenergic receptors. J. Biol. Chem [print-electronic]. 2003 Aug 8/22/2003; 278(34): 32405-12. PMID: 12738775, PII: M304195200, DOI: 10.1074/jbc.M304195200, ISSN: 0021-9258.

Brady AE, Limbird LE. G protein-coupled receptor interacting proteins: emerging roles in localization and signal transduction. Cell. Signal. 2002 Apr; 14(4): 297-309. PMID: 11858937, PII: S089865680100239X, ISSN: 0898-6568.

Richman JG, Brady AE, Wang Q, Hensel JL, Colbran RJ, Limbird LE. Agonist-regulated Interaction between alpha2-adrenergic receptors and spinophilin. J. Biol. Chem [print-electronic]. 2001 May 5/4/2001; 276(18): 15003-8. PMID: 11154706, PII: M011679200, DOI: 10.1074/jbc.M011679200, ISSN: 0021-9258.