Faculty Appointments
Director of Undergraduate Studies in Chemical and Biomolecular Engineering Associate Professor of Chemical & Biomolecular Engineering
Associate Professor of NeurologyAssociate Professor of NeurologyAssociate Professor of Biomedical EngineeringAssociate Professor of Biomedical Engineering
Education
Ph.D., Chemical Engineering, University of Wisconsin, Madison, WisconsinB.S., Chemical Engineering, University of Illinois, Urbana-Champaign, Illinois
Research Description
We operate at the intersection of engineering and regenerative medicine with the goal of understanding neurovascular diseases (e.g. disorders that afflict the central nervous system and its surrounding blood vessels) and designing rational therapeutic strategies to combat their progression. Our lab utilizes a diverse spectrum of experimental techniques, including human pluripotent stem cell (hPSC) differentiation, genetic engineering, microscale fabrication, and molecular selection/directed evolution. Our platform technologies are intended to facilitate the development of representative tissue models, the design and selection of novel targeting agents and signaling pathway modulators, and the construction of advanced high throughput systems for drug screening and biological discovery.
Research Keywords
Human pluripotent stem cells, neurological disease, tissue engineering, biomedical engineering, biomolecular engineering
Publications
Leng K, Rose IVL, Kim H, Xia W, Romero-Fernandez W, Rooney B, Koontz M, Li E, Ao Y, Wang S, Krawczyk M, Tcw J, Goate A, Zhang Y, Ullian EM, Sofroniew MV, Fancy SPJ, Schrag MS, Lippmann ES, Kampmann M. CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states. Nat Neurosci [print-electronic]. 2022 Nov; 25(11): 1528-42. PMID: 36303069, PII: 10.1038/s41593-022-01180-9, DOI: 10.1038/s41593-022-01180-9, ISSN: 1546-1726.
Shi Y, Kim H, Hamann CA, Rhea EM, Brunger JM, Lippmann ES. Nuclear receptor ligand screening in an iPSC-derived in vitro blood-brain barrier model identifies new contributors to leptin transport. Fluids Barriers CNS. 2022 Sep 9/21/2022; 19(1): 77. PMID: 36131285, PMCID: PMC9494897, PII: 10.1186/s12987-022-00375-3, DOI: 10.1186/s12987-022-00375-3, ISSN: 2045-8118.
O'Grady BJ, Geuy MD, Kim H, Balotin KM, Allchin ER, Florian DC, Bute NN, Scott TE, Lowen GB, Fricker CM, Fitzgerald ML, Guelcher SA, Wikswo JP, Bellan LM, Lippmann ES. Rapid prototyping of cell culture microdevices using parylene-coated 3D prints. Lab Chip. 2021 Dec 12/7/2021; 21(24): 4814-22. PMID: 34787148, PMCID: PMC8717820, DOI: 10.1039/d1lc00744k, ISSN: 1473-0189.
Sorets AG, Rosch JC, Duvall CL, Lippmann ES. Caveolae-Mediated Transport at the Injured Blood-Brain Barrier as an Underexplored Pathway for Central Nervous System Drug Delivery. Curr Opin Chem Eng [print-electronic]. 2020 Dec; 30: 86-95. PMID: 32953427, PMCID: PMC7497790, DOI: 10.1016/j.coche.2020.08.009, ISSN: 2211-3398.
O'Grady BJ, Balotin KM, Bosworth AM, McClatchey PM, Weinstein RM, Gupta M, Poole KS, Bellan LM, Lippmann ES. Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks. ACS Biomater Sci Eng [print-electronic]. 2020 Oct 10/12/2020; 6(10): 5811-22. PMID: 33320550, PMCID: PMC7791574, DOI: 10.1021/acsbiomaterials.0c00885, ISSN: 2373-9878.
Neal EH, Marinelli NA, Shi Y, McClatchey PM, Balotin KM, Gullett DR, Hagerla KA, Bowman AB, Ess KC, Wikswo JP, Lippmann ES. A Simplified, Fully Defined Differentiation Scheme for Producing Blood-Brain Barrier Endothelial Cells from Human iPSCs. Stem Cell Reports. 2019 Jun 6/11/2019; 12(6): 1380-8. PMID: 31189096, PMCID: PMC6565873, PII: S2213-6711(19)30175-4, DOI: 10.1016/j.stemcr.2019.05.008, ISSN: 2213-6711.
Stebbins MJ, Lippmann ES, Faubion MG, Daneman R, Palecek SP, Shusta EV. Activation of RARa, RAR¿, or RXRa Increases Barrier Tightness in Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells. Biotechnol J [print-electronic]. 2017 Sep 9/28/2017; PMID: 28960887, DOI: 10.1002/biot.201700093, ISSN: 1860-7314.
Hollmann EK, Bailey AK, Potharazu AV, Neely MD, Bowman AB, Lippmann ES. Accelerated differentiation of human induced pluripotent stem cells to blood-brain barrier endothelial cells. Fluids Barriers CNS. 2017 Apr 4/13/2017; 14(1): 9. PMID: 28407791, PMCID: PMC5390351, PII: 10.1186/s12987-017-0059-0, DOI: 10.1186/s12987-017-0059-0, ISSN: 2045-8118.
Bosworth AM, Faley SL, Bellan LM, Lippmann ES. Modeling Neurovascular Disorders and Therapeutic Outcomes with Human-Induced Pluripotent Stem Cells. Front Bioeng Biotechnol. 2017; 5: 87. PMID: 29441348, PMCID: PMC5797533, DOI: 10.3389/fbioe.2017.00087, ISSN: 2296-4185.
Rosch JC, Hollmann EK, Lippmann ES. In vitro selection technologies to enhance biomaterial functionality. Exp. Biol. Med. (Maywood) [print-electronic]. 2016 May; 241(9): 962-71. PMID: 27188514, PMCID: PMC4950355, PII: 1535370216647182, DOI: 10.1177/1535370216647182, ISSN: 1535-3699.
Rosch JC, Hollmann EK, Lippmann ES. In vitro selection technologies to enhance biomaterial functionality. Exp. Biol. Med. (Maywood) [print-electronic]. 2016 May; 241(9): 962-71. PMID: 27188514, PMCID: PMC4950355, PII: 1535370216647182, DOI: 10.1177/1535370216647182, ISSN: 1535-3699.
Lippmann ES, Williams CE, Ruhl DA, Estevez-Silva MC, Chapman ER, Coon JJ, Ashton RS. Deterministic HOX patterning in human pluripotent stem cell-derived neuroectoderm. Stem Cell Reports [print-electronic]. 2015 Apr 4/14/2015; 4(4): 632-44. PMID: 25843047, PMCID: PMC4400649, PII: S2213-6711(15)00074-0, DOI: 10.1016/j.stemcr.2015.02.018, ISSN: 2213-6711.
Lippmann ES, Estevez-Silva MC, Ashton RS. Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors. Stem Cells. 2014 Apr; 32(4): 1032-42. PMID: 24357014, DOI: 10.1002/stem.1622, ISSN: 1549-4918.
Sha J, Lippmann ES, McNulty J, Ma Y, Ashton RS. Sequential nucleophilic substitutions permit orthogonal click functionalization of multicomponent PEG brushes. Biomacromolecules [print-electronic]. 2013 Sep 9/9/2013; 14(9): 3294-303. PMID: 23937610, DOI: 10.1021/bm400900r, ISSN: 1526-4602.
Lippmann ES, Al-Ahmad A, Palecek SP, Shusta EV. Modeling the blood-brain barrier using stem cell sources. Fluids Barriers CNS. 2013; 10(1): 2. PMID: 23305164, PMCID: PMC3564868, PII: 2045-8118-10-2, DOI: 10.1186/2045-8118-10-2, ISSN: 2045-8118.
Lippmann ES, Azarin SM, Kay JE, Nessler RA, Wilson HK, Al-Ahmad A, Palecek SP, Shusta EV. Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells. Nat. Biotechnol. 2012 Aug; 30(8): 783-91. PMID: 22729031, PMCID: PMC3467331, PII: nbt.2247, DOI: 10.1038/nbt.2247, ISSN: 1546-1696.
Lippmann ES, Weidenfeller C, Svendsen CN, Shusta EV. Blood-brain barrier modeling with co-cultured neural progenitor cell-derived astrocytes and neurons. J. Neurochem [print-electronic]. 2011 Nov; 119(3): 507-20. PMID: 21854389, PMCID: PMC3192906, DOI: 10.1111/j.1471-4159.2011.07434.x, ISSN: 1471-4159.
Agarwal N, Lippmann ES, Shusta EV. Identification and expression profiling of blood-brain barrier membrane proteins. J. Neurochem [print-electronic]. 2010 Feb; 112(3): 625-35. PMID: 19895664, PMCID: PMC2809131, PII: JNC6481, DOI: 10.1111/j.1471-4159.2009.06481.x, ISSN: 1471-4159.
Shi Y, Kim H, Hamann CA, Rhea EM, Brunger JM, Lippmann ES. Nuclear receptor ligand screening in an iPSC-derived in vitro blood-brain barrier model identifies new contributors to leptin transport. Fluids Barriers CNS. 2022 Sep 9/21/2022; 19(1): 77. PMID: 36131285, PMCID: PMC9494897, PII: 10.1186/s12987-022-00375-3, DOI: 10.1186/s12987-022-00375-3, ISSN: 2045-8118.
O'Grady BJ, Geuy MD, Kim H, Balotin KM, Allchin ER, Florian DC, Bute NN, Scott TE, Lowen GB, Fricker CM, Fitzgerald ML, Guelcher SA, Wikswo JP, Bellan LM, Lippmann ES. Rapid prototyping of cell culture microdevices using parylene-coated 3D prints. Lab Chip. 2021 Dec 12/7/2021; 21(24): 4814-22. PMID: 34787148, PMCID: PMC8717820, DOI: 10.1039/d1lc00744k, ISSN: 1473-0189.
Sorets AG, Rosch JC, Duvall CL, Lippmann ES. Caveolae-Mediated Transport at the Injured Blood-Brain Barrier as an Underexplored Pathway for Central Nervous System Drug Delivery. Curr Opin Chem Eng [print-electronic]. 2020 Dec; 30: 86-95. PMID: 32953427, PMCID: PMC7497790, DOI: 10.1016/j.coche.2020.08.009, ISSN: 2211-3398.
O'Grady BJ, Balotin KM, Bosworth AM, McClatchey PM, Weinstein RM, Gupta M, Poole KS, Bellan LM, Lippmann ES. Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks. ACS Biomater Sci Eng [print-electronic]. 2020 Oct 10/12/2020; 6(10): 5811-22. PMID: 33320550, PMCID: PMC7791574, DOI: 10.1021/acsbiomaterials.0c00885, ISSN: 2373-9878.
Neal EH, Marinelli NA, Shi Y, McClatchey PM, Balotin KM, Gullett DR, Hagerla KA, Bowman AB, Ess KC, Wikswo JP, Lippmann ES. A Simplified, Fully Defined Differentiation Scheme for Producing Blood-Brain Barrier Endothelial Cells from Human iPSCs. Stem Cell Reports. 2019 Jun 6/11/2019; 12(6): 1380-8. PMID: 31189096, PMCID: PMC6565873, PII: S2213-6711(19)30175-4, DOI: 10.1016/j.stemcr.2019.05.008, ISSN: 2213-6711.
Stebbins MJ, Lippmann ES, Faubion MG, Daneman R, Palecek SP, Shusta EV. Activation of RARa, RAR¿, or RXRa Increases Barrier Tightness in Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells. Biotechnol J [print-electronic]. 2017 Sep 9/28/2017; PMID: 28960887, DOI: 10.1002/biot.201700093, ISSN: 1860-7314.
Hollmann EK, Bailey AK, Potharazu AV, Neely MD, Bowman AB, Lippmann ES. Accelerated differentiation of human induced pluripotent stem cells to blood-brain barrier endothelial cells. Fluids Barriers CNS. 2017 Apr 4/13/2017; 14(1): 9. PMID: 28407791, PMCID: PMC5390351, PII: 10.1186/s12987-017-0059-0, DOI: 10.1186/s12987-017-0059-0, ISSN: 2045-8118.
Bosworth AM, Faley SL, Bellan LM, Lippmann ES. Modeling Neurovascular Disorders and Therapeutic Outcomes with Human-Induced Pluripotent Stem Cells. Front Bioeng Biotechnol. 2017; 5: 87. PMID: 29441348, PMCID: PMC5797533, DOI: 10.3389/fbioe.2017.00087, ISSN: 2296-4185.
Rosch JC, Hollmann EK, Lippmann ES. In vitro selection technologies to enhance biomaterial functionality. Exp. Biol. Med. (Maywood) [print-electronic]. 2016 May; 241(9): 962-71. PMID: 27188514, PMCID: PMC4950355, PII: 1535370216647182, DOI: 10.1177/1535370216647182, ISSN: 1535-3699.
Rosch JC, Hollmann EK, Lippmann ES. In vitro selection technologies to enhance biomaterial functionality. Exp. Biol. Med. (Maywood) [print-electronic]. 2016 May; 241(9): 962-71. PMID: 27188514, PMCID: PMC4950355, PII: 1535370216647182, DOI: 10.1177/1535370216647182, ISSN: 1535-3699.
Lippmann ES, Williams CE, Ruhl DA, Estevez-Silva MC, Chapman ER, Coon JJ, Ashton RS. Deterministic HOX patterning in human pluripotent stem cell-derived neuroectoderm. Stem Cell Reports [print-electronic]. 2015 Apr 4/14/2015; 4(4): 632-44. PMID: 25843047, PMCID: PMC4400649, PII: S2213-6711(15)00074-0, DOI: 10.1016/j.stemcr.2015.02.018, ISSN: 2213-6711.
Lippmann ES, Estevez-Silva MC, Ashton RS. Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors. Stem Cells. 2014 Apr; 32(4): 1032-42. PMID: 24357014, DOI: 10.1002/stem.1622, ISSN: 1549-4918.
Sha J, Lippmann ES, McNulty J, Ma Y, Ashton RS. Sequential nucleophilic substitutions permit orthogonal click functionalization of multicomponent PEG brushes. Biomacromolecules [print-electronic]. 2013 Sep 9/9/2013; 14(9): 3294-303. PMID: 23937610, DOI: 10.1021/bm400900r, ISSN: 1526-4602.
Lippmann ES, Al-Ahmad A, Palecek SP, Shusta EV. Modeling the blood-brain barrier using stem cell sources. Fluids Barriers CNS. 2013; 10(1): 2. PMID: 23305164, PMCID: PMC3564868, PII: 2045-8118-10-2, DOI: 10.1186/2045-8118-10-2, ISSN: 2045-8118.
Lippmann ES, Azarin SM, Kay JE, Nessler RA, Wilson HK, Al-Ahmad A, Palecek SP, Shusta EV. Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells. Nat. Biotechnol. 2012 Aug; 30(8): 783-91. PMID: 22729031, PMCID: PMC3467331, PII: nbt.2247, DOI: 10.1038/nbt.2247, ISSN: 1546-1696.
Lippmann ES, Weidenfeller C, Svendsen CN, Shusta EV. Blood-brain barrier modeling with co-cultured neural progenitor cell-derived astrocytes and neurons. J. Neurochem [print-electronic]. 2011 Nov; 119(3): 507-20. PMID: 21854389, PMCID: PMC3192906, DOI: 10.1111/j.1471-4159.2011.07434.x, ISSN: 1471-4159.
Agarwal N, Lippmann ES, Shusta EV. Identification and expression profiling of blood-brain barrier membrane proteins. J. Neurochem [print-electronic]. 2010 Feb; 112(3): 625-35. PMID: 19895664, PMCID: PMC2809131, PII: JNC6481, DOI: 10.1111/j.1471-4159.2009.06481.x, ISSN: 1471-4159.