Charles F. Spurlock, III, Ph.D.

Research Instructor
Faculty Appointments
Research Instructor in Medicine
Ph.D., Microbiology and Immunology, Vanderbilt University, Nashville, TennesseeB.S., Biology, University of the South, Sewanee, Tennessee
Office Address
LH 1225C
Nashville, TN 37232
Research Keywords
long non-coding RNAs, autoimmune disease, RNA biology, RNA sequencing, adaptive immune system, T cells, gene regulation
Spurlock CF, Shaginurova G, Tossberg JT, Hester JD, Chapman N, Guo Y, Crooke PS, Aune TM. Profiles of Long Noncoding RNAs in Human Naive and Memory T Cells. J. Immunol [print-electronic]. 2017 Jun 6/9/2017; PMID: 28600289, PII: jimmunol.1700232, DOI: 10.4049/jimmunol.1700232, ISSN: 1550-6606.

Aune TM, Crooke PS, Patrick AE, Tossberg JT, Olsen NJ, Spurlock CF. Expression of long non-coding RNAs in autoimmunity and linkage to enhancer function and autoimmune disease risk genetic variants. J. Autoimmun [print-electronic]. 2017 Apr 4/15/2017; PMID: 28420548, PII: S0896-8411(17)30070-7, DOI: 10.1016/j.jaut.2017.03.014, ISSN: 1095-9157.

Spurlock CF, Crooke PS, Aune TM. Biogenesis and Transcriptional Regulation of Long Noncoding RNAs in the Human Immune System. J. Immunol. 2016 Dec 12/15/2016; 197(12): 4509-17. PMID: 27913643, PMCID: PMC5140008, PII: 197/12/4509, DOI: 10.4049/jimmunol.1600970, ISSN: 1550-6606.

Aune TM, Crooke PS, Spurlock CF. Long noncoding RNAs in T lymphocytes. J. Leukoc. Biol [print-electronic]. 2016 Jan; 99(1): 31-44. PMID: 26538526, PII: jlb.1RI0815-389R, DOI: 10.1189/jlb.1RI0815-389R, ISSN: 1938-3673.

Spurlock CF, Tossberg JT, Olsen NJ, Aune TM. Cutting Edge: Chronic NF-kB Activation in CD4+ T Cells in Rheumatoid Arthritis Is Genetically Determined by HLA Risk Alleles. J. Immunol. 2015 Aug 8/1/2015; 195(3): 791-5. PMID: 26091715, PMCID: PMC4506868, PII: jimmunol.1500267, DOI: 10.4049/jimmunol.1500267, ISSN: 1550-6606.

Aune TM, Spurlock CF. Long non-coding RNAs in innate and adaptive immunity. Virus Res [print-electronic]. 2015 Jul 7/9/2015; PMID: 26166759, PII: S0168-1702(15)30007-1, DOI: 10.1016/j.virusres.2015.07.003, ISSN: 1872-7492.

Spurlock CF, Tossberg JT, Guo Y, Collier SP, Crooke PS, Aune TM. Expression and functions of long noncoding RNAs during human T helper cell differentiation. Nat Commun. 2015; 6: 6932. PMID: 25903499, PMCID: PMC4410435, PII: ncomms7932, DOI: 10.1038/ncomms7932, ISSN: 2041-1723.

Spurlock CF, Tossberg JT, Guo Y, Sriram S, Crooke PS, Aune TM. Defective structural RNA processing in relapsing-remitting multiple sclerosis. Genome Biol. 2015; 16: 58. PMID: 25885816, PMCID: PMC4403723, PII: 10.1186/s13059-015-0629-x, DOI: 10.1186/s13059-015-0629-x, ISSN: 1474-760X.

Spurlock CF, Gass HM, Bryant CJ, Wells BC, Olsen NJ, Aune TM. Methotrexate-mediated inhibition of nuclear factor kB activation by distinct pathways in T cells and fibroblast-like synoviocytes. Rheumatology (Oxford). 2015 Jan; 54(1): 178-87. PMID: 25118313, PMCID: PMC4269792, PII: keu279, DOI: 10.1093/rheumatology/keu279, ISSN: 1462-0332.

Spurlock, CF, Olsen, NJ, Aune, TM.. Will Understanding Methotrexate Modes of Action Teach us About Rheumatoid Arthritis?. [place unknown: publisher unknown]; 2015. Available from:

Spurlock CF, Tossberg JT, Matlock BK, Olsen NJ, Aune TM. Methotrexate inhibits NF-kB activity via long intergenic (noncoding) RNA-p21 induction. Arthritis & rheumatology (Hoboken, N.J.). 2014 Nov; 66(11): 2947-57. PMID: 25077978, PMCID: PMC4211976, DOI: 10.1002/art.38805, ISSN: 2326-5205.

Zhang F, Tossberg JT, Spurlock CF, Yao SY, Aune TM, Sriram S. Expression of IL-33 and its epigenetic regulation in Multiple Sclerosis. Ann Clin Transl Neurol. 2014 May 5/1/2014; 1(5): 307-18. PMID: 25215310, PMCID: PMC4157667, DOI: 10.1002/acn3.47, ISSN: 2328-9503.

Spurlock CF, Tossberg JT, Fuchs HA, Olsen NJ, Aune TM. Methotrexate increases expression of cell cycle checkpoint genes via JNK activation. Arthritis Rheum [print-electronic]. 2012 Jun; 64(6): 1780-9. PMID: 22183962, PMCID: PMC3310965, DOI: 10.1002/art.34342, ISSN: 1529-0131.

Spurlock CF, Aune ZT, Tossberg JT, Collins PL, Aune JP, Huston JW, Crooke PS, Olsen NJ, Aune TM. Increased sensitivity to apoptosis induced by methotrexate is mediated by JNK. Arthritis Rheum. 2011 Sep; 63(9): 2606-16. PMID: 21618198, PMCID: PMC3165146, DOI: 10.1002/art.30457, ISSN: 1529-0131.