Faculty Appointments
Assistant Professor of Medicine
Assistant Professor of Cell & Developmental Biology
Education
Ph.D., Cell Biology and Anatomy, Medical College of Georgia, Augusta, GeorgiaB.S., Biology, Emmanuel College , Franklin Sp, Georgia
Publications
Lynch MR, Tran MT, Ralto KM, Zsengeller ZK, Raman V, Bhasin SS, Sun N, Chen X, Brown D, Rovira II, Taguchi K, Brooks CR, Stillman IE, Bhasin MK, Finkel T, Parikh SM. TFEB-driven lysosomal biogenesis is pivotal for PGC1a-dependent renal stress resistance. JCI Insight. 2019 Mar 3/14/2019; 5: PMID: 30870143, PII: 126749, DOI: 10.1172/jci.insight.126749, ISSN: 2379-3708.
Canaud G, Brooks CR, Kishi S, Taguchi K, Nishimura K, Magassa S, Scott A, Hsiao LL, Ichimura T, Terzi F, Yang L, Bonventre JV. Cyclin G1 and TASCC regulate kidney epithelial cell G2-M arrest and fibrotic maladaptive repair. Sci Transl Med. 2019 Jan 1/23/2019; 11(476): PMID: 30674655, PMCID: PMC6527117, PII: 11/476/eaav4754, DOI: 10.1126/scitranslmed.aav4754, ISSN: 1946-6242.
Taguchi K, Yamagishi SI, Yokoro M, Ito S, Kodama G, Kaida Y, Nakayama Y, Ando R, Yamada-Obara N, Asanuma K, Matsui T, Higashimoto Y, Brooks CR, Ueda S, Okuda S, Fukami K. RAGE-aptamer attenuates deoxycorticosterone acetate/salt-induced renal injury in mice. Sci Rep. 2018 Feb 2/8/2018; 8(1): 2686. PMID: 29422652, PMCID: PMC5805738, PII: 10.1038/s41598-018-21176-5, DOI: 10.1038/s41598-018-21176-5, ISSN: 2045-2322.
Kim YK, Refaeli I, Brooks CR, Jing P, Gulieva RE, Hughes MR, Cruz NM, Liu Y, Churchill AJ, Wang Y, Fu H, Pippin JW, Lin LY, Shankland SJ, Vogl AW, McNagny KM, Freedman BS. Gene-Edited Human Kidney Organoids Reveal Mechanisms of Disease in Podocyte Development. Stem Cells [print-electronic]. 2017 Dec; 35(12): 2366-78. PMID: 28905451, PMCID: PMC5742857, DOI: 10.1002/stem.2707, ISSN: 1549-4918.
Brooks CR, Bonventre JV. KIM-1/TIM-1 in proximal tubular cell immune response [editorial]. Oncotarget. 2015 Dec 12/29/2015; 6(42): 44059-60. PMID: 26683362, PMCID: PMC4792540, PII: 6623, DOI: 10.18632/oncotarget.6623, ISSN: 1949-2553.
Freedman BS, Brooks CR, Lam AQ, Fu H, Morizane R, Agrawal V, Saad AF, Li MK, Hughes MR, Werff RV, Peters DT, Lu J, Baccei A, Siedlecki AM, Valerius MT, Musunuru K, McNagny KM, Steinman TI, Zhou J, Lerou PH, Bonventre JV. Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids. Nat Commun. 2015 Oct 10/23/2015; 6: 8715. PMID: 26493500, PMCID: PMC4620584, PII: ncomms9715, DOI: 10.1038/ncomms9715, ISSN: 2041-1723.
Brooks CR, Yeung MY, Brooks YS, Chen H, Ichimura T, Henderson JM, Bonventre JV. KIM-1-/TIM-1-mediated phagocytosis links ATG5-/ULK1-dependent clearance of apoptotic cells to antigen presentation. EMBO J [print-electronic]. 2015 Oct 10/1/2015; 34(19): 2441-64. PMID: 26282792, PMCID: PMC4601664, PII: embj.201489838, DOI: 10.15252/embj.201489838, ISSN: 0261-4189.
Kishi S, Campanholle G, Gohil VM, Perocchi F, Brooks CR, Morizane R, Sabbisetti V, Ichimura T, Mootha VK, Bonventre JV. Meclizine Preconditioning Protects the Kidney Against Ischemia-Reperfusion Injury. EBioMedicine. 2015 Sep; 2(9): 1090-101. PMID: 26501107, PMCID: PMC4588407, PII: S2352-3964(15)30087-6, DOI: 10.1016/j.ebiom.2015.07.035.
Yang L, Brooks CR, Xiao S, Sabbisetti V, Yeung MY, Hsiao LL, Ichimura T, Kuchroo V, Bonventre JV. KIM-1-mediated phagocytosis reduces acute injury to the kidney. J. Clin. Invest [print-electronic]. 2015 Apr; 125(4): 1620-36. PMID: 25751064, PMCID: PMC4396492, PII: 75417, DOI: 10.1172/JCI75417, ISSN: 1558-8238.
Xiao S, Brooks CR, Sobel RA, Kuchroo VK. Tim-1 is essential for induction and maintenance of IL-10 in regulatory B cells and their regulation of tissue inflammation. J. Immunol [print-electronic]. 2015 Feb 2/15/2015; 194(4): 1602-8. PMID: 25582854, PMCID: PMC4346345, PII: jimmunol.1402632, DOI: 10.4049/jimmunol.1402632, ISSN: 1550-6606.
Zhan M, Brooks C, Liu F, Sun L, Dong Z. Mitochondrial dynamics: regulatory mechanisms and emerging role in renal pathophysiology. Kidney Int [print-electronic]. 2013 Apr; 83(4): 568-81. PMID: 23325082, PMCID: PMC3612360, PII: S0085-2538(15)55790-6, DOI: 10.1038/ki.2012.441, ISSN: 1523-1755.
Xiao S, Brooks CR, Zhu C, Wu C, Sweere JM, Petecka S, Yeste A, Quintana FJ, Ichimura T, Sobel RA, Bonventre JV, Kuchroo VK. Defect in regulatory B-cell function and development of systemic autoimmunity in T-cell Ig mucin 1 (Tim-1) mucin domain-mutant mice. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Jul 7/24/2012; 109(30): 12105-10. PMID: 22773818, PMCID: PMC3409739, PII: 1120914109, DOI: 10.1073/pnas.1120914109, ISSN: 1091-6490.
Ichimura T, Brooks CR, Bonventre JV. Kim-1/Tim-1 and immune cells: shifting sands. Kidney Int. 2012 May; 81(9): 809-11. PMID: 22499138, PMCID: PMC3339439, PII: S0085-2538(15)55413-6, DOI: 10.1038/ki.2012.11, ISSN: 1523-1755.
Grgic I, Brooks CR, Hofmeister AF, Bijol V, Bonventre JV, Humphreys BD. Imaging of podocyte foot processes by fluorescence microscopy. J. Am. Soc. Nephrol [print-electronic]. 2012 May; 23(5): 785-91. PMID: 22362911, PMCID: PMC3338299, PII: ASN.2011100988, DOI: 10.1681/ASN.2011100988, ISSN: 1533-3450.
Brooks C, Cho SG, Wang CY, Yang T, Dong Z. Fragmented mitochondria are sensitized to Bax insertion and activation during apoptosis. Am. J. Physiol., Cell Physiol [print-electronic]. 2011 Mar; 300(3): C447-55. PMID: 21160028, PMCID: PMC3063970, PII: ajpcell.00402.2010, DOI: 10.1152/ajpcell.00402.2010, ISSN: 1522-1563.
Kaddour-Djebbar I, Choudhary V, Brooks C, Ghazaly T, Lakshmikanthan V, Dong Z, Kumar MV. Specific mitochondrial calcium overload induces mitochondrial fission in prostate cancer cells. Int. J. Oncol. 2010 Jun; 36(6): 1437-44. PMID: 20428767, ISSN: 1791-2423.
Yang L, Besschetnova TY, Brooks CR, Shah JV, Bonventre JV. Epithelial cell cycle arrest in G2/M mediates kidney fibrosis after injury. Nat. Med [print-electronic]. 2010 May; 16(5): 535-43, 1p following 143. PMID: 20436483, PMCID: PMC3928013, PII: nm.2144, DOI: 10.1038/nm.2144, ISSN: 1546-170X.
Brooks C, Wei Q, Cho SG, Dong Z. Regulation of mitochondrial dynamics in acute kidney injury in cell culture and rodent models. J. Clin. Invest [print-electronic]. 2009 May; 119(5): 1275-85. PMID: 19349686, PMCID: PMC2673870, PII: 37829, DOI: 10.1172/JCI37829, ISSN: 1558-8238.
Yang D, Wang S, Brooks C, Dong Z, Schoenlein PV, Kumar V, Ouyang X, Xiong H, Lahat G, Hayes-Jordan A, Lazar A, Pollock R, Lev D, Liu K. IFN regulatory factor 8 sensitizes soft tissue sarcoma cells to death receptor-initiated apoptosis via repression of FLICE-like protein expression. Cancer Res [print-electronic]. 2009 Feb 2/1/2009; 69(3): 1080-8. PMID: 19155307, PMCID: PMC2633427, PII: 0008-5472.CAN-08-2520, DOI: 10.1158/0008-5472.CAN-08-2520, ISSN: 1538-7445.
Brooks C, Dong Z. Regulation of mitochondrial morphological dynamics during apoptosis by Bcl-2 family proteins: a key in Bak?. Cell Cycle [print-electronic]. 2007 Dec 12/15/2007; 6(24): 3043-7. PMID: 18073534, PII: 5115, DOI: 10.4161/cc.6.24.5115, ISSN: 1551-4005.
Brooks C, Wei Q, Feng L, Dong G, Tao Y, Mei L, Xie ZJ, Dong Z. Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2007 Jul 7/10/2007; 104(28): 11649-54. PMID: 17606912, PMCID: PMC1913853, PII: 0703976104, DOI: 10.1073/pnas.0703976104, ISSN: 0027-8424.
Brooks C, Wang J, Yang T, Dong Z. Characterization of cell clones isolated from hypoxia-selected renal proximal tubular cells. Am. J. Physiol. Renal Physiol [print-electronic]. 2007 Jan; 292(1): F243-52. PMID: 16885151, PII: 00236.2006, DOI: 10.1152/ajprenal.00236.2006, ISSN: 1931-857X.
Brooks C, Ketsawatsomkron P, Sui Y, Wang J, Wang CY, Yu FS, Dong Z. Acidic pH inhibits ATP depletion-induced tubular cell apoptosis by blocking caspase-9 activation in apoptosome. Am. J. Physiol. Renal Physiol [print-electronic]. 2005 Aug; 289(2): F410-9. PMID: 15755925, PII: 00440.2004, DOI: 10.1152/ajprenal.00440.2004, ISSN: 1931-857X.
Canaud G, Brooks CR, Kishi S, Taguchi K, Nishimura K, Magassa S, Scott A, Hsiao LL, Ichimura T, Terzi F, Yang L, Bonventre JV. Cyclin G1 and TASCC regulate kidney epithelial cell G2-M arrest and fibrotic maladaptive repair. Sci Transl Med. 2019 Jan 1/23/2019; 11(476): PMID: 30674655, PMCID: PMC6527117, PII: 11/476/eaav4754, DOI: 10.1126/scitranslmed.aav4754, ISSN: 1946-6242.
Taguchi K, Yamagishi SI, Yokoro M, Ito S, Kodama G, Kaida Y, Nakayama Y, Ando R, Yamada-Obara N, Asanuma K, Matsui T, Higashimoto Y, Brooks CR, Ueda S, Okuda S, Fukami K. RAGE-aptamer attenuates deoxycorticosterone acetate/salt-induced renal injury in mice. Sci Rep. 2018 Feb 2/8/2018; 8(1): 2686. PMID: 29422652, PMCID: PMC5805738, PII: 10.1038/s41598-018-21176-5, DOI: 10.1038/s41598-018-21176-5, ISSN: 2045-2322.
Kim YK, Refaeli I, Brooks CR, Jing P, Gulieva RE, Hughes MR, Cruz NM, Liu Y, Churchill AJ, Wang Y, Fu H, Pippin JW, Lin LY, Shankland SJ, Vogl AW, McNagny KM, Freedman BS. Gene-Edited Human Kidney Organoids Reveal Mechanisms of Disease in Podocyte Development. Stem Cells [print-electronic]. 2017 Dec; 35(12): 2366-78. PMID: 28905451, PMCID: PMC5742857, DOI: 10.1002/stem.2707, ISSN: 1549-4918.
Brooks CR, Bonventre JV. KIM-1/TIM-1 in proximal tubular cell immune response [editorial]. Oncotarget. 2015 Dec 12/29/2015; 6(42): 44059-60. PMID: 26683362, PMCID: PMC4792540, PII: 6623, DOI: 10.18632/oncotarget.6623, ISSN: 1949-2553.
Freedman BS, Brooks CR, Lam AQ, Fu H, Morizane R, Agrawal V, Saad AF, Li MK, Hughes MR, Werff RV, Peters DT, Lu J, Baccei A, Siedlecki AM, Valerius MT, Musunuru K, McNagny KM, Steinman TI, Zhou J, Lerou PH, Bonventre JV. Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids. Nat Commun. 2015 Oct 10/23/2015; 6: 8715. PMID: 26493500, PMCID: PMC4620584, PII: ncomms9715, DOI: 10.1038/ncomms9715, ISSN: 2041-1723.
Brooks CR, Yeung MY, Brooks YS, Chen H, Ichimura T, Henderson JM, Bonventre JV. KIM-1-/TIM-1-mediated phagocytosis links ATG5-/ULK1-dependent clearance of apoptotic cells to antigen presentation. EMBO J [print-electronic]. 2015 Oct 10/1/2015; 34(19): 2441-64. PMID: 26282792, PMCID: PMC4601664, PII: embj.201489838, DOI: 10.15252/embj.201489838, ISSN: 0261-4189.
Kishi S, Campanholle G, Gohil VM, Perocchi F, Brooks CR, Morizane R, Sabbisetti V, Ichimura T, Mootha VK, Bonventre JV. Meclizine Preconditioning Protects the Kidney Against Ischemia-Reperfusion Injury. EBioMedicine. 2015 Sep; 2(9): 1090-101. PMID: 26501107, PMCID: PMC4588407, PII: S2352-3964(15)30087-6, DOI: 10.1016/j.ebiom.2015.07.035.
Yang L, Brooks CR, Xiao S, Sabbisetti V, Yeung MY, Hsiao LL, Ichimura T, Kuchroo V, Bonventre JV. KIM-1-mediated phagocytosis reduces acute injury to the kidney. J. Clin. Invest [print-electronic]. 2015 Apr; 125(4): 1620-36. PMID: 25751064, PMCID: PMC4396492, PII: 75417, DOI: 10.1172/JCI75417, ISSN: 1558-8238.
Xiao S, Brooks CR, Sobel RA, Kuchroo VK. Tim-1 is essential for induction and maintenance of IL-10 in regulatory B cells and their regulation of tissue inflammation. J. Immunol [print-electronic]. 2015 Feb 2/15/2015; 194(4): 1602-8. PMID: 25582854, PMCID: PMC4346345, PII: jimmunol.1402632, DOI: 10.4049/jimmunol.1402632, ISSN: 1550-6606.
Zhan M, Brooks C, Liu F, Sun L, Dong Z. Mitochondrial dynamics: regulatory mechanisms and emerging role in renal pathophysiology. Kidney Int [print-electronic]. 2013 Apr; 83(4): 568-81. PMID: 23325082, PMCID: PMC3612360, PII: S0085-2538(15)55790-6, DOI: 10.1038/ki.2012.441, ISSN: 1523-1755.
Xiao S, Brooks CR, Zhu C, Wu C, Sweere JM, Petecka S, Yeste A, Quintana FJ, Ichimura T, Sobel RA, Bonventre JV, Kuchroo VK. Defect in regulatory B-cell function and development of systemic autoimmunity in T-cell Ig mucin 1 (Tim-1) mucin domain-mutant mice. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2012 Jul 7/24/2012; 109(30): 12105-10. PMID: 22773818, PMCID: PMC3409739, PII: 1120914109, DOI: 10.1073/pnas.1120914109, ISSN: 1091-6490.
Ichimura T, Brooks CR, Bonventre JV. Kim-1/Tim-1 and immune cells: shifting sands. Kidney Int. 2012 May; 81(9): 809-11. PMID: 22499138, PMCID: PMC3339439, PII: S0085-2538(15)55413-6, DOI: 10.1038/ki.2012.11, ISSN: 1523-1755.
Grgic I, Brooks CR, Hofmeister AF, Bijol V, Bonventre JV, Humphreys BD. Imaging of podocyte foot processes by fluorescence microscopy. J. Am. Soc. Nephrol [print-electronic]. 2012 May; 23(5): 785-91. PMID: 22362911, PMCID: PMC3338299, PII: ASN.2011100988, DOI: 10.1681/ASN.2011100988, ISSN: 1533-3450.
Brooks C, Cho SG, Wang CY, Yang T, Dong Z. Fragmented mitochondria are sensitized to Bax insertion and activation during apoptosis. Am. J. Physiol., Cell Physiol [print-electronic]. 2011 Mar; 300(3): C447-55. PMID: 21160028, PMCID: PMC3063970, PII: ajpcell.00402.2010, DOI: 10.1152/ajpcell.00402.2010, ISSN: 1522-1563.
Kaddour-Djebbar I, Choudhary V, Brooks C, Ghazaly T, Lakshmikanthan V, Dong Z, Kumar MV. Specific mitochondrial calcium overload induces mitochondrial fission in prostate cancer cells. Int. J. Oncol. 2010 Jun; 36(6): 1437-44. PMID: 20428767, ISSN: 1791-2423.
Yang L, Besschetnova TY, Brooks CR, Shah JV, Bonventre JV. Epithelial cell cycle arrest in G2/M mediates kidney fibrosis after injury. Nat. Med [print-electronic]. 2010 May; 16(5): 535-43, 1p following 143. PMID: 20436483, PMCID: PMC3928013, PII: nm.2144, DOI: 10.1038/nm.2144, ISSN: 1546-170X.
Brooks C, Wei Q, Cho SG, Dong Z. Regulation of mitochondrial dynamics in acute kidney injury in cell culture and rodent models. J. Clin. Invest [print-electronic]. 2009 May; 119(5): 1275-85. PMID: 19349686, PMCID: PMC2673870, PII: 37829, DOI: 10.1172/JCI37829, ISSN: 1558-8238.
Yang D, Wang S, Brooks C, Dong Z, Schoenlein PV, Kumar V, Ouyang X, Xiong H, Lahat G, Hayes-Jordan A, Lazar A, Pollock R, Lev D, Liu K. IFN regulatory factor 8 sensitizes soft tissue sarcoma cells to death receptor-initiated apoptosis via repression of FLICE-like protein expression. Cancer Res [print-electronic]. 2009 Feb 2/1/2009; 69(3): 1080-8. PMID: 19155307, PMCID: PMC2633427, PII: 0008-5472.CAN-08-2520, DOI: 10.1158/0008-5472.CAN-08-2520, ISSN: 1538-7445.
Brooks C, Dong Z. Regulation of mitochondrial morphological dynamics during apoptosis by Bcl-2 family proteins: a key in Bak?. Cell Cycle [print-electronic]. 2007 Dec 12/15/2007; 6(24): 3043-7. PMID: 18073534, PII: 5115, DOI: 10.4161/cc.6.24.5115, ISSN: 1551-4005.
Brooks C, Wei Q, Feng L, Dong G, Tao Y, Mei L, Xie ZJ, Dong Z. Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2007 Jul 7/10/2007; 104(28): 11649-54. PMID: 17606912, PMCID: PMC1913853, PII: 0703976104, DOI: 10.1073/pnas.0703976104, ISSN: 0027-8424.
Brooks C, Wang J, Yang T, Dong Z. Characterization of cell clones isolated from hypoxia-selected renal proximal tubular cells. Am. J. Physiol. Renal Physiol [print-electronic]. 2007 Jan; 292(1): F243-52. PMID: 16885151, PII: 00236.2006, DOI: 10.1152/ajprenal.00236.2006, ISSN: 1931-857X.
Brooks C, Ketsawatsomkron P, Sui Y, Wang J, Wang CY, Yu FS, Dong Z. Acidic pH inhibits ATP depletion-induced tubular cell apoptosis by blocking caspase-9 activation in apoptosome. Am. J. Physiol. Renal Physiol [print-electronic]. 2005 Aug; 289(2): F410-9. PMID: 15755925, PII: 00440.2004, DOI: 10.1152/ajprenal.00440.2004, ISSN: 1931-857X.
Available Postdoctoral Position Details
Posted: 10/24/2016
Position: Postdoctoral fellow in the cell biology of kidney injury.
Description: We are seeking a highly motivated postdoctoral fellow intent on a career in academic research to join the Brooks lab in the Division of Nephrology and Hypertension (https://labnodes.vanderbilt.edu/member/profile/id/12317). The ideal applicant will have PhD in Cell Biology, Genetics, or related field and experience with animal models of kidney injury (surgical and nephrotoxic), mouse husbandry, monitoring kidney function (serum creatinine and BUN measurements), molecular biology (PCR, plasmid construction and transfection), tissue culture, biochemistry (immunoblotting and immunostaining) and data analysis.
A significant portion of the research conducted in our group focuses on understanding the role of cell cycle arrest in the progression of fibrosis in the kidney, leading to chronic kidney disease. We have previously published that proximal tubular cells arrested in the G2/M phase of the cell cycle contribute to fibrosis through incompletely defined mechanisms. Through multiplatform microarray analysis, we have identified cell cycle arrest mediators which regulate the G2/M phase of cell cycle and will examine the role of these cell cycle regulators in acute and chronic injuries to the kidney. Other projects include: Delineating the mechanism and therapeutic potential of autophagy activation in the regulation of immune processes in AKI; and examining if mitochondrial dynamics proteins, such as mitofusins, modulate autophagic flux in AKI. There are multiple opportunities to lead projects and to participate in ongoing studies. The projected start date is flexible with intent to have the position filled by Fall 2016.
Qualified applicants should submit a cover letter describing previous research experience as well as current interests, curriculum vitae and contact details of three references to Craig Brooks, Ph.D. by email at craig.brooks@vanderbilt.edu.