Faculty Appointments
Associate Professor of Medicine
Associate Professor of DermatologyAssociate Professor of Pathology, Microbiology and ImmunologyAssociate Professor of Pharmacology
Education
Ph.D., Immunology, Institute of Physics - University of Sao Paulo, Sao Paulo, Brazil
Office Address
MCN A2310A
Nashville, TN 37232
Nashville, TN 37232
Research Description
The Serezani laboratory aims to develop therapeutic strategies to control systemic (sepsis) and localized infections (skin and lung) in healthy individuals, individuals with immune deficiencies, and those suffering from preexisting diseases, such as diabetes, obesity and aging. We have been concentrating much of our efforts in understanding how phagocytes (macrophages and neutrophils) behave and influence the outcome of infections in vivo. We have pioneered studies dissecting abnormal neutrophil actions in the site of infection and dissecting signaling programs elicited by pathogen recognition receptors in the skin. We also have been intensively investigating how immunometabolism influence epigenetic changes during sepsis. Our lab employs state of the art techniques to understand in vitro and in vivo cellular and microbial dynamics, such as intravital microscopy and IVIS imaging, along with different fluorescent transgenic and cell-specific deficient mice to track cell and organ-specific events.
Research Keywords
Diabetes, obesity, skin infection, sepsis. innate immunity, macrophage, neutrophil, host defense, microRNAs, lung injury, epigenetic, immunometabolism SOCS-1, abscess, immunotherapy
Publications
De Melo P, Pineros Alvarez AR, Ye X, Blackman A, Alves-Filho JC, Medeiros AI, Rathmell J, Pua H, Serezani CH. Macrophage-Derived MicroRNA-21 Drives Overwhelming Glycolytic and Inflammatory Response during Sepsis via Repression of the PGE2/IL-10 Axis. J Immunol [print-electronic]. 2021 Aug 8/1/2021; 207(3): 902-12. PMID: 34301845, PMCID: PMC8323968, PII: jimmunol.2001251, DOI: 10.4049/jimmunol.2001251, ISSN: 1550-6606.
Mehrpouya-Bahrami P, Moriarty AK, De Melo P, Keeter WC, Alakhras NS, Nelson AS, Hoover M, Barrios MS, Nadler JL, Serezani CH, Kaplan MH, Galkina EV. STAT4 is expressed in neutrophils and promotes antimicrobial immunity. JCI Insight. 2021 Jul 7/22/2021; 6(14): PMID: 34138758, PMCID: PMC8410094, PII: e141326, DOI: 10.1172/jci.insight.141326, ISSN: 2379-3708.
Bonyek-Silva I, Machado AFA, Cerqueira-Silva T, Nunes S, Silva Cruz MR, Silva J, Santos RL, Barral A, Oliveira PRS, Khouri R, Serezani CH, Brodskyn C, Caldas JR, Barral-Netto M, Boaventura V, Tavares NM. LTB4-Driven Inflammation and Increased Expression of ALOX5/ACE2 During Severe COVID-19 in Individuals With Diabetes. Diabetes [print-electronic]. 2021 Jun 6/15/2021; PMID: 34417262, PII: db20-1260, DOI: 10.2337/db20-1260, ISSN: 1939-327X.
Lyu H, Elkins CM, Pierce JL, Serezani CH, Perrien DS. MyD88 Is Not Required for Muscle Injury-Induced Endochondral Heterotopic Ossification in a Mouse Model of Fibrodysplasia Ossificans Progressiva. Biomedicines. 2021 Jun 6/1/2021; 9(6): PMID: 34206078, PMCID: PMC8227787, PII: biomedicines9060630, DOI: 10.3390/biomedicines9060630, ISSN: 2227-9059.
Klopfenstein N, Cassat JE, Monteith A, Miller A, Drury S, Skaar E, Serezani CH. Murine Models for Staphylococcal Infection. Curr Protoc. 2021 Mar; 1(3): e52. PMID: 33656290, PMCID: PMC7935403, DOI: 10.1002/cpz1.52, ISSN: 2691-1299.
Klopfenstein N, Brandt SL, Castellanos S, Gunzer M, Blackman A, Serezani CH. SOCS-1 inhibition of type I interferon restrains Staphylococcus aureus skin host defense. PLoS Pathog. 2021 Mar; 17(3): e1009387. PMID: 33690673, PMCID: PMC7984627, PII: PPATHOGENS-D-20-02265, DOI: 10.1371/journal.ppat.1009387, ISSN: 1553-7374.
Chastain CA, Klopfenstein N, Serezani CH, Aronoff DM. A Clinical Review of Diabetic Foot Infections. Clin Podiatr Med Surg [print-electronic]. 2019 Jul; 36(3): 381-95. PMID: 31079605, PII: S0891-8422(19)30016-3, DOI: 10.1016/j.cpm.2019.02.004, ISSN: 1558-2302.
Brandt SL, Wang S, Dejani NN, Klopfenstein N, Winfree S, Filgueiras L, McCarthy BP, Territo PR, Serezani CH. Excessive localized leukotriene B4 levels dictate poor skin host defense in diabetic mice. JCI Insight [print-electronic]. 2018 Sep 9/6/2018; 3(17): PMID: 30185672, PII: 120220, DOI: 10.1172/jci.insight.120220, ISSN: 2379-3708.
Dejani NN, Orlando AB, Niño VE, Penteado LA, Verdan FF, Bazzano JMR, Codo AC, Salina ACG, Saraiva AC, Avelar MR, Spolidorio LC, Serezani CH, Medeiros AI. Intestinal host defense outcome is dictated by PGE2 production during efferocytosis of infected cells. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2018 Sep 9/4/2018; 115(36): E8469-E8478. PMID: 30127026, PMCID: PMC6130380, PII: 1722016115, DOI: 10.1073/pnas.1722016115, ISSN: 1091-6490.
Brandt SL, Klopfenstein N, Wang S, Winfree S, McCarthy BP, Territo PR, Miller L, Serezani CH. Macrophage-derived LTB4 promotes abscess formation and clearance of Staphylococcus aureus skin infection in mice. PLoS Pathog. 2018 Aug; 14(8): e1007244. PMID: 30102746, PMCID: PMC6107286, PII: PPATHOGENS-D-18-00195, DOI: 10.1371/journal.ppat.1007244, ISSN: 1553-7374.
Brandt SL, Putnam NE, Cassat JE, Serezani CH. Innate Immunity to Staphylococcus aureus: Evolving Paradigms in Soft Tissue and Invasive Infections. J. Immunol. 2018 Jun 6/15/2018; 200(12): 3871-80. PMID: 29866769, PMCID: PMC6028009, PII: 200/12/3871, DOI: 10.4049/jimmunol.1701574, ISSN: 1550-6606.
Sisti F, Wang S, Brandt SL, Glosson-Byers N, Mayo LD, Son YM, Sturgeon S, Filgueiras L, Jancar S, Wong H, Dela Cruz CS, Andrews N, Alves-Filho JC, Cunha FQ, Serezani CH. Nuclear PTEN enhances the maturation of a microRNA regulon to limit MyD88-dependent susceptibility to sepsis. Sci Signal. 2018 May 5/1/2018; 11(528): PMID: 29717063, PII: 11/528/eaai9085, DOI: 10.1126/scisignal.aai9085, ISSN: 1937-9145.
Braga Filho JAF, Abreu AG, Rios CEP, Trovão LO, Silva DLF, Cysne DN, Nascimento JR, Fortes TS, Silva LA, Guerra RNM, Maciel MCG, Serezani CH, Nascimento FRF. Prophylactic Treatment With Simvastatin Modulates the Immune Response and Increases Animal Survival Following Lethal Sepsis Infection. Front Immunol. 2018; 9: 2137. PMID: 30298072, PMCID: PMC6160584, DOI: 10.3389/fimmu.2018.02137, ISSN: 1664-3224.
Brandt SL, Serezani CH. Too much of a good thing: How modulating LTB4 actions restore host defense in homeostasis or disease. Semin. Immunol. 2017 Oct; 33: 37-43. PMID: 29042027, PMCID: PMC5679129, PII: S1044-5323(16)30079-3, DOI: 10.1016/j.smim.2017.08.006, ISSN: 1096-3618.
Piñeros Alvarez AR, Glosson-Byers N, Brandt S, Wang S, Wong H, Sturgeon S, McCarthy BP, Territo PR, Alves-Filho JC, Serezani CH. SOCS1 is a negative regulator of metabolic reprogramming during sepsis. JCI Insight [print-electronic]. 2017 Jul 7/6/2017; 2(13): PMID: 28679957, PMCID: PMC5499360, PII: 92530, DOI: 10.1172/jci.insight.92530, ISSN: 2379-3708.
Salina AC, Souza TP, Serezani CH, Medeiros AI. Efferocytosis-induced prostaglandin E2 production impairs alveolar macrophage effector functions during Streptococcus pneumoniae infection. Innate Immun [print-electronic]. 2017 Apr; 23(3): 219-27. PMID: 28359217, DOI: 10.1177/1753425916684934, ISSN: 1753-4267.
Dejani NN, Brandt SL, Piñeros A, Glosson-Byers NL, Wang S, Son YM, Medeiros AI, Serezani CH. Topical Prostaglandin E Analog Restores Defective Dendritic Cell-Mediated Th17 Host Defense Against Methicillin-Resistant Staphylococcus Aureus in the Skin of Diabetic Mice. Diabetes [print-electronic]. 2016 Sep 9/7/2016; PMID: 27605625, PII: db16-0565, DOI: 10.2337/db16-0565, ISSN: 1939-327X.
Filgueiras LR, Brandt SL, Ramalho TR, Jancar S, Serezani CH. Imbalance between HDAC and HAT activities drives aberrant STAT1/MyD88 expression in macrophages from type 1 diabetic mice. J. Diabetes Complicat [print-electronic]. 2016 Aug 8/7/2016; PMID: 27623388, PII: S1056-8727(16)30341-5, DOI: 10.1016/j.jdiacomp.2016.08.001, ISSN: 1873-460X.
Li XJ, Deng L, Brandt SL, Goodwin CB, Ma P, Yang Z, Mali RS, Liu Z, Kapur R, Serezani CH, Chan RJ. Role of p85a in neutrophil extra- and intracellular reactive oxygen species generation. Oncotarget. 2016 Apr 4/26/2016; 7(17): 23096-105. PMID: 27049833, PII: 8500, DOI: 10.18632/oncotarget.8500, ISSN: 1949-2553.
Zoccal KF, Sorgi CA, Hori JI, Paula-Silva FW, Arantes EC, Serezani CH, Zamboni DS, Faccioli LH. Opposing roles of LTB4 and PGE2 in regulating the inflammasome-dependent scorpion venom-induced mortality. Nat Commun. 2016; 7: 10760. PMID: 26907476, PMCID: PMC4766425, PII: ncomms10760, DOI: 10.1038/ncomms10760, ISSN: 2041-1723.
Ishizuka EK, Filgueiras LR, Rios FJ, Serezani CH, Jancar S. PAFR activation of NF-¿B p65 or p105 precursor dictates pro- and anti-inflammatory responses during TLR activation in murine macrophages. Sci Rep. 2016; 6: 32092. PMID: 27554194, PMCID: PMC4995467, PII: srep32092, DOI: 10.1038/srep32092, ISSN: 2045-2322.
Carrasco SE, Troxell B, Yang Y, Brandt SL, Li H, Sandusky GE, Condon KW, Serezani CH, Yang XF. Outer surface protein OspC is an antiphagocytic factor that protects Borrelia burgdorferi from phagocytosis by macrophages. Infect. Immun [print-electronic]. 2015 Dec; 83(12): 4848-60. PMID: 26438793, PMCID: PMC4645385, PII: IAI.01215-15, DOI: 10.1128/IAI.01215-15, ISSN: 1098-5522.
Filgueiras LR, Brandt SL, Wang S, Wang Z, Morris DL, Evans-Molina C, Mirmira RG, Jancar S, Serezani CH. Leukotriene B4-mediated sterile inflammation promotes susceptibility to sepsis in a mouse model of type 1 diabetes. Sci Signal. 2015 Jan 1/27/2015; 8(361): ra10. PMID: 25628460, PMCID: PMC4356178, PII: 8/361/ra10, DOI: 10.1126/scisignal.2005568, ISSN: 1937-9145.
Filgueiras LR, Serezani CH, Jancar S. Leukotriene B4 as a Potential Therapeutic Target for the Treatment of Metabolic Disorders. Front Immunol. 2015; 6: 515. PMID: 26500652, PMCID: PMC4597104, DOI: 10.3389/fimmu.2015.00515, ISSN: 1664-3224.
Wang Z, Brandt S, Medeiros A, Wang S, Wu H, Dent A, Serezani CH. MicroRNA 21 is a homeostatic regulator of macrophage polarization and prevents prostaglandin E2-mediated M2 generation. PLoS ONE. 2015; 10(2): e0115855. PMID: 25706647, PMCID: PMC4338261, PII: PONE-D-14-41738, DOI: 10.1371/journal.pone.0115855, ISSN: 1932-6203.
Ribeiro LR, Loures FV, de Araújo EF, Feriotti C, Costa TA, Serezani CH, Jancar S, Calich VL. Lipoxin Inhibits Fungal Uptake by Macrophages and Reduces the Severity of Acute Pulmonary Infection Caused by Paracoccidioides brasiliensis. Mediators Inflamm [print-electronic]. 2015; 2015: 852574. PMID: 26635449, PMCID: PMC4618125, DOI: 10.1155/2015/852574, ISSN: 1466-1861.
Morato CI, da Silva IA, Borges AF, Dorta ML, Oliveira MA, Jancar S, Serezani CH, Ribeiro-Dias F. Essential role of leukotriene B4 on Leishmania (Viannia) braziliensis killing by human macrophages. Microbes Infect [print-electronic]. 2014 Nov; 16(11): 945-53. PMID: 25195516, PII: S1286-4579(14)00134-8, DOI: 10.1016/j.micinf.2014.08.015, ISSN: 1769-714X.
da Silva-Souza HA, Lira MN, Costa-Junior HM, da Cruz CM, Vasconcellos JS, Mendes AN, Pimenta-Reis G, Alvarez CL, Faccioli LH, Serezani CH, Schachter J, Persechini PM. Inhibitors of the 5-lipoxygenase arachidonic acid pathway induce ATP release and ATP-dependent organic cation transport in macrophages. Biochim. Biophys. Acta [print-electronic]. 2014 Jul; 1838(7): 1967-77. PMID: 24743022, PII: S0005-2736(14)00141-2, DOI: 10.1016/j.bbamem.2014.04.006, ISSN: 0006-3002.
Ferreira AE, Sisti F, Sônego F, Wang S, Filgueiras LR, Brandt S, Serezani AP, Du H, Cunha FQ, Alves-Filho JC, Serezani CH. PPAR-¿/IL-10 axis inhibits MyD88 expression and ameliorates murine polymicrobial sepsis. J. Immunol [print-electronic]. 2014 Mar 3/1/2014; 192(5): 2357-65. PMID: 24489087, PMCID: PMC3943997, PII: jimmunol.1302375, DOI: 10.4049/jimmunol.1302375, ISSN: 1550-6606.
Wang Z, Filgueiras LR, Wang S, Serezani AP, Peters-Golden M, Jancar S, Serezani CH. Leukotriene B4 enhances the generation of proinflammatory microRNAs to promote MyD88-dependent macrophage activation. J. Immunol [print-electronic]. 2014 Mar 3/1/2014; 192(5): 2349-56. PMID: 24477912, PMCID: PMC3943984, PII: jimmunol.1302982, DOI: 10.4049/jimmunol.1302982, ISSN: 1550-6606.
Secatto A, Soares EM, Locachevic GA, Assis PA, Paula-Silva FW, Serezani CH, de Medeiros AI, Faccioli LH. The leukotriene B4/BLT1 axis is a key determinant in susceptibility and resistance to histoplasmosis. PLoS ONE. 2014; 9(1): e85083. PMID: 24465479, PMCID: PMC3897419, PII: PONE-D-13-25916, DOI: 10.1371/journal.pone.0085083, ISSN: 1932-6203.
Rogers LM, Thelen T, Fordyce K, Bourdonnay E, Lewis C, Yu H, Zhang J, Xie J, Serezani CH, Peters-Golden M, Aronoff DM. EP4 and EP2 receptor activation of protein kinase A by prostaglandin E2 impairs macrophage phagocytosis of Clostridium sordellii. Am. J. Reprod. Immunol [print-electronic]. 2014 Jan; 71(1): 34-43. PMID: 23902376, PMCID: PMC3864121, DOI: 10.1111/aji.12153, ISSN: 1600-0897.
Domingo-Gonzalez R, Katz S, Serezani CH, Moore TA, Levine AM, Moore BB. Prostaglandin E2-induced changes in alveolar macrophage scavenger receptor profiles differentially alter phagocytosis of Pseudomonas aeruginosa and Staphylococcus aureus post-bone marrow transplant. J. Immunol [print-electronic]. 2013 Jun 6/1/2013; 190(11): 5809-17. PMID: 23630358, PMCID: PMC3660503, PII: jimmunol.1203274, DOI: 10.4049/jimmunol.1203274, ISSN: 1550-6606.
Hoggatt J, Mohammad KS, Singh P, Hoggatt AF, Chitteti BR, Speth JM, Hu P, Poteat BA, Stilger KN, Ferraro F, Silberstein L, Wong FK, Farag SS, Czader M, Milne GL, Breyer RM, Serezani CH, Scadden DT, Guise TA, Srour EF, Pelus LM. Differential stem- and progenitor-cell trafficking by prostaglandin E2. Nature [print-electronic]. 2013 Mar 3/21/2013; 495(7441): 365-9. PMID: 23485965, PMCID: PMC3606692, PII: nature11929, DOI: 10.1038/nature11929, ISSN: 1476-4687.
Soares EM, Mason KL, Rogers LM, Serezani CH, Faccioli LH, Aronoff DM. Leukotriene B4 enhances innate immune defense against the puerperal sepsis agent Streptococcus pyogenes. J. Immunol [print-electronic]. 2013 Feb 2/15/2013; 190(4): 1614-22. PMID: 23325886, PMCID: PMC3563855, PII: jimmunol.1202932, DOI: 10.4049/jimmunol.1202932, ISSN: 1550-6606.
Simões RL, Arruda MA, Canetti C, Serezani CH, Fierro IM, Barja-Fidalgo C. Proinflammatory responses of heme in alveolar macrophages: repercussion in lung hemorrhagic episodes. Mediators Inflamm [print-electronic]. 2013; 2013: 946878. PMID: 23690673, PMCID: PMC3652176, DOI: 10.1155/2013/946878, ISSN: 1466-1861.
Mor-Vaknin N, Legendre M, Yu Y, Serezani CH, Garg SK, Jatzek A, Swanson MD, Gonzalez-Hernandez MJ, Teitz-Tennenbaum S, Punturieri A, Engleberg NC, Banerjee R, Peters-Golden M, Kao JY, Markovitz DM. Murine colitis is mediated by vimentin. Sci Rep [print-electronic]. 2013; 3: 1045. PMID: 23304436, PMCID: PMC3540396, DOI: 10.1038/srep01045, ISSN: 2045-2322.
Serezani CH, Kane S, Collins L, Morato-Marques M, Osterholzer JJ, Peters-Golden M. Macrophage dectin-1 expression is controlled by leukotriene B4 via a GM-CSF/PU.1 axis. J. Immunol [print-electronic]. 2012 Jul 7/15/2012; 189(2): 906-15. PMID: 22696442, PMCID: PMC3392366, PII: jimmunol.1200257, DOI: 10.4049/jimmunol.1200257, ISSN: 1550-6606.
Mancuso P, Myers MG, Goel D, Serezani CH, O'Brien E, Goldberg J, Aronoff DM, Peters-Golden M. Ablation of leptin receptor-mediated ERK activation impairs host defense against Gram-negative pneumonia. J. Immunol [print-electronic]. 2012 Jul 7/15/2012; 189(2): 867-75. PMID: 22685316, PMCID: PMC3392451, PII: jimmunol.1200465, DOI: 10.4049/jimmunol.1200465, ISSN: 1550-6606.
Bourdonnay E, Serezani CH, Aronoff DM, Peters-Golden M. Regulation of alveolar macrophage p40phox: hierarchy of activating kinases and their inhibition by PGE2. J. Leukoc. Biol [print-electronic]. 2012 Jul; 92(1): 219-31. PMID: 22544939, PMCID: PMC3382311, PII: jlb.1211590, DOI: 10.1189/jlb.1211590, ISSN: 1938-3673.
Zaslona Z, Serezani CH, Okunishi K, Aronoff DM, Peters-Golden M. Prostaglandin E2 restrains macrophage maturation via E prostanoid receptor 2/protein kinase A signaling. Blood [print-electronic]. 2012 Mar 3/8/2012; 119(10): 2358-67. PMID: 22234697, PMCID: PMC3311259, PII: blood-2011-08-374207, DOI: 10.1182/blood-2011-08-374207, ISSN: 1528-0020.
Serezani CH, Kane S, Medeiros AI, Cornett AM, Kim SH, Marques MM, Lee SP, Lewis C, Bourdonnay E, Ballinger MN, White ES, Peters-Golden M. PTEN directly activates the actin depolymerization factor cofilin-1 during PGE2-mediated inhibition of phagocytosis of fungi. Sci Signal. 2012 Feb 2/7/2012; 5(210): ra12. PMID: 22317922, PMCID: PMC3400468, PII: 5/210/ra12, DOI: 10.1126/scisignal.2002448, ISSN: 1937-9145.
Filgueiras LR, Martins JO, Serezani CH, Capelozzi VL, Montes MB, Jancar S. Sepsis-induced acute lung injury (ALI) is milder in diabetic rats and correlates with impaired NFkB activation. PLoS ONE [print-electronic]. 2012; 7(9): e44987. PMID: 23024779, PMCID: PMC3443211, PII: PONE-D-12-14042, DOI: 10.1371/journal.pone.0044987, ISSN: 1932-6203.
Medeiros A, Peres-Buzalaf C, Fortino Verdan F, Serezani CH. Prostaglandin E2 and the suppression of phagocyte innate immune responses in different organs. Mediators Inflamm [print-electronic]. 2012; 2012: 327568. PMID: 23024463, PMCID: PMC3449139, DOI: 10.1155/2012/327568, ISSN: 1466-1861.
Secatto A, Rodrigues LC, Serezani CH, Ramos SG, Dias-Baruffi M, Faccioli LH, Medeiros AI. 5-Lipoxygenase deficiency impairs innate and adaptive immune responses during fungal infection. PLoS ONE [print-electronic]. 2012; 7(3): e31701. PMID: 22448213, PMCID: PMC3308949, PII: PONE-D-11-09055, DOI: 10.1371/journal.pone.0031701, ISSN: 1932-6203.
Morato-Marques M, Campos MR, Kane S, Rangel AP, Lewis C, Ballinger MN, Kim SH, Peters-Golden M, Jancar S, Serezani CH. Leukotrienes target F-actin/cofilin-1 to enhance alveolar macrophage anti-fungal activity. J. Biol. Chem [print-electronic]. 2011 Aug 8/19/2011; 286(33): 28902-13. PMID: 21715328, PMCID: PMC3190697, PII: M111.235309, DOI: 10.1074/jbc.M111.235309, ISSN: 1083-351X.
Kim SH, Serezani CH, Okunishi K, Zaslona Z, Aronoff DM, Peters-Golden M. Distinct protein kinase A anchoring proteins direct prostaglandin E2 modulation of Toll-like receptor signaling in alveolar macrophages. J. Biol. Chem [print-electronic]. 2011 Mar 3/18/2011; 286(11): 8875-83. PMID: 21247892, PMCID: PMC3058957, PII: M110.187815, DOI: 10.1074/jbc.M110.187815, ISSN: 1083-351X.
Serezani CH, Lewis C, Jancar S, Peters-Golden M. Leukotriene B4 amplifies NF-¿B activation in mouse macrophages by reducing SOCS1 inhibition of MyD88 expression. J. Clin. Invest [print-electronic]. 2011 Feb; 121(2): 671-82. PMID: 21206089, PMCID: PMC3026722, PII: 43302, DOI: 10.1172/JCI43302, ISSN: 1558-8238.
Thelen T, Hao Y, Medeiros AI, Curtis JL, Serezani CH, Kobzik L, Harris LH, Aronoff DM. The class A scavenger receptor, macrophage receptor with collagenous structure, is the major phagocytic receptor for Clostridium sordellii expressed by human decidual macrophages. J. Immunol [print-electronic]. 2010 Oct 10/1/2010; 185(7): 4328-35. PMID: 20810988, PII: jimmunol.1000989, DOI: 10.4049/jimmunol.1000989, ISSN: 1550-6606.
Mancuso P, Lewis C, Serezani CH, Goel D, Peters-Golden M. Intrapulmonary administration of leukotriene B4 enhances pulmonary host defense against pneumococcal pneumonia. Infect. Immun [print-electronic]. 2010 May; 78(5): 2264-71. PMID: 20231413, PMCID: PMC2863513, PII: IAI.01323-09, DOI: 10.1128/IAI.01323-09, ISSN: 1098-5522.
Serezani CH, Aronoff DM, Sitrin RG, Peters-Golden M. FcgammaRI ligation leads to a complex with BLT1 in lipid rafts that enhances rat lung macrophage antimicrobial functions. Blood [print-electronic]. 2009 Oct 10/8/2009; 114(15): 3316-24. PMID: 19657115, PMCID: PMC2759654, PII: blood-2009-01-199919, DOI: 10.1182/blood-2009-01-199919, ISSN: 1528-0020.
Aronoff DM, Lewis C, Serezani CH, Eaton KA, Goel D, Phipps JC, Peters-Golden M, Mancuso P. E-prostanoid 3 receptor deletion improves pulmonary host defense and protects mice from death in severe Streptococcus pneumoniae infection. J. Immunol [print-electronic]. 2009 Aug 8/15/2009; 183(4): 2642-9. PMID: 19635910, PMCID: PMC4016992, PII: jimmunol.0900129, DOI: 10.4049/jimmunol.0900129, ISSN: 1550-6606.
Campos MR, Serezani CH, Peters-Golden M, Jancar S. Differential kinase requirement for enhancement of Fc gammaR-mediated phagocytosis in alveolar macrophages by leukotriene B4 vs. D4. Mol. Immunol [print-electronic]. 2009 Mar; 46(6): 1204-11. PMID: 19223078, PII: S0161-5890(08)00791-8, DOI: 10.1016/j.molimm.2008.11.024, ISSN: 1872-9142.
Medeiros AI, Serezani CH, Lee SP, Peters-Golden M. Efferocytosis impairs pulmonary macrophage and lung antibacterial function via PGE2/EP2 signaling. J. Exp. Med [print-electronic]. 2009 Jan 1/16/2009; 206(1): 61-8. PMID: 19124657, PMCID: PMC2626688, PII: jem.20082058, DOI: 10.1084/jem.20082058, ISSN: 1540-9538.
Lee SP, Serezani CH, Medeiros AI, Ballinger MN, Peters-Golden M. Crosstalk between prostaglandin E2 and leukotriene B4 regulates phagocytosis in alveolar macrophages via combinatorial effects on cyclic AMP. J. Immunol. 2009 Jan 1/1/2009; 182(1): 530-7. PMID: 19109185, PII: 182/1/530, ISSN: 1550-6606.
Costa-Junior HM, Mendes AN, Davis GH, da Cruz CM, Ventura AL, Serezani CH, Faccioli LH, Nomizo A, Freire-de-Lima CG, Bisaggio Rda C, Persechini PM. ATP-induced apoptosis involves a Ca2+-independent phospholipase A2 and 5-lipoxygenase in macrophages. Prostaglandins Other Lipid Mediat [print-electronic]. 2009 Jan; 88(1-2): 51-61. PMID: 18984060, PII: S1098-8823(08)00067-1, DOI: 10.1016/j.prostaglandins.2008.09.004, ISSN: 1098-8823.
Medeiros AI, Sá-Nunes A, Turato WM, Secatto A, Frantz FG, Sorgi CA, Serezani CH, Deepe GS, Faccioli LH. Leukotrienes are potent adjuvant during fungal infection: effects on memory T cells. J. Immunol. 2008 Dec 12/15/2008; 181(12): 8544-51. PMID: 19050273, PII: 181/12/8544, ISSN: 1550-6606.
Weinlich R, Bortoluci KR, Chehab CF, Serezani CH, Ulbrich AG, Peters-Golden M, Russo M, Amarante-Mendes GP. TLR4/MYD88-dependent, LPS-induced synthesis of PGE2 by macrophages or dendritic cells prevents anti-CD3-mediated CD95L upregulation in T cells. Cell Death Differ [print-electronic]. 2008 Dec; 15(12): 1901-9. PMID: 18820644, PII: cdd2008128, DOI: 10.1038/cdd.2008.128, ISSN: 1476-5403.
Chung J, Serezani CH, Huang SK, Stern JN, Keskin DB, Jagirdar R, Brock TG, Aronoff DM, Peters-Golden M. Rap1 activation is required for Fc gamma receptor-dependent phagocytosis. J. Immunol. 2008 Oct 10/15/2008; 181(8): 5501-9. PMID: 18832707, PMCID: PMC3077557, PII: 181/8/5501, ISSN: 1550-6606.
Serezani CH, Ballinger MN, Aronoff DM, Peters-Golden M. Cyclic AMP: master regulator of innate immune cell function. Am. J. Respir. Cell Mol. Biol [print-electronic]. 2008 Aug; 39(2): 127-32. PMID: 18323530, PMCID: PMC2720142, PII: 2008-0091TR, DOI: 10.1165/rcmb.2008-0091TR, ISSN: 1535-4989.
Aronoff DM, Hao Y, Chung J, Coleman N, Lewis C, Peres CM, Serezani CH, Chen GH, Flamand N, Brock TG, Peters-Golden M. Misoprostol impairs female reproductive tract innate immunity against Clostridium sordellii. J. Immunol. 2008 Jun 6/15/2008; 180(12): 8222-30. PMID: 18523288, PMCID: PMC2667109, PII: 180/12/8222, ISSN: 0022-1767.
Brock TG, Serezani CH, Carstens JK, Peters-Golden M, Aronoff DM. Effects of prostaglandin E2 on the subcellular localization of Epac-1 and Rap1 proteins during Fcgamma-receptor-mediated phagocytosis in alveolar macrophages. Exp. Cell Res [print-electronic]. 2008 Jan 1/15/2008; 314(2): 255-63. PMID: 18021770, PMCID: PMC2390918, PII: S0014-4827(07)00491-0, DOI: 10.1016/j.yexcr.2007.10.011, ISSN: 0014-4827.
Canetti C, Serezani CH, Atrasz RG, White ES, Aronoff DM, Peters-Golden M. Activation of phosphatase and tensin homolog on chromosome 10 mediates the inhibition of FcgammaR phagocytosis by prostaglandin E2 in alveolar macrophages. J. Immunol. 2007 Dec 12/15/2007; 179(12): 8350-6. PMID: 18056380, PII: 179/12/8350, ISSN: 0022-1767.
Coffey MJ, Serezani CH, Phare SM, Flamand N, Peters-Golden M. NADPH oxidase deficiency results in reduced alveolar macrophage 5-lipoxygenase expression and decreased leukotriene synthesis. J. Leukoc. Biol [print-electronic]. 2007 Dec; 82(6): 1585-91. PMID: 17761955, PII: jlb.0107019, DOI: 10.1189/jlb.0107019, ISSN: 0741-5400.
Serezani CH, Chung J, Ballinger MN, Moore BB, Aronoff DM, Peters-Golden M. Prostaglandin E2 suppresses bacterial killing in alveolar macrophages by inhibiting NADPH oxidase. Am. J. Respir. Cell Mol. Biol [print-electronic]. 2007 Nov; 37(5): 562-70. PMID: 17585108, PMCID: PMC2048683, PII: 2007-0153OC, DOI: 10.1165/rcmb.2007-0153OC, ISSN: 1535-4989.
Peres CM, Aronoff DM, Serezani CH, Flamand N, Faccioli LH, Peters-Golden M. Specific leukotriene receptors couple to distinct G proteins to effect stimulation of alveolar macrophage host defense functions. J. Immunol. 2007 Oct 10/15/2007; 179(8): 5454-61. PMID: 17911632, PII: 179/8/5454, ISSN: 0022-1767.
Flamand N, Mancuso P, Serezani CH, Brock TG. Leukotrienes: mediators that have been typecast as villains. Cell. Mol. Life Sci. 2007 Oct; 64(19-20): 2657-70. PMID: 17639273, DOI: 10.1007/s00018-007-7228-2, ISSN: 1420-682X.
Aronoff DM, Peres CM, Serezani CH, Ballinger MN, Carstens JK, Coleman N, Moore BB, Peebles RS, Faccioli LH, Peters-Golden M. Synthetic prostacyclin analogs differentially regulate macrophage function via distinct analog-receptor binding specificities. J. Immunol. 2007 Feb 2/1/2007; 178(3): 1628-34. PMID: 17237412, PII: 178/3/1628, ISSN: 0022-1767.
Aronoff DM, Serezani CH, Carstens JK, Marshall T, Gangireddy SR, Peters-Golden M, Reddy RC. Stimulatory Effects of Peroxisome Proliferator-Activated Receptor-gamma on Fcgamma Receptor-Mediated Phagocytosis by Alveolar Macrophages. PPAR Res. 2007; 2007: 52546. PMID: 18253476, PMCID: PMC2220046, DOI: 10.1155/2007/52546, ISSN: 1687-4757.
Serezani CH, Perrela JH, Russo M, Peters-Golden M, Jancar S. Leukotrienes are essential for the control of Leishmania amazonensis infection and contribute to strain variation in susceptibility. J. Immunol. 2006 Sep 9/1/2006; 177(5): 3201-8. PMID: 16920959, PII: 177/5/3201, ISSN: 0022-1767.
Ballinger MN, Paine R, Serezani CH, Aronoff DM, Choi ES, Standiford TJ, Toews GB, Moore BB. Role of granulocyte macrophage colony-stimulating factor during gram-negative lung infection with Pseudomonas aeruginosa. Am. J. Respir. Cell Mol. Biol [print-electronic]. 2006 Jun; 34(6): 766-74. PMID: 16474098, PMCID: PMC2644237, PII: 2005-0246OC, DOI: 10.1165/rcmb.2005-0246OC, ISSN: 1044-1549.
Serezani CH, Aronoff DM, Jancar S, Peters-Golden M. Leukotriene B4 mediates p47phox phosphorylation and membrane translocation in polyunsaturated fatty acid-stimulated neutrophils. J. Leukoc. Biol [print-electronic]. 2005 Oct; 78(4): 976-84. PMID: 16006535, PII: jlb.1004587, DOI: 10.1189/jlb.1004587, ISSN: 0741-5400.
Serezani CH, Aronoff DM, Jancar S, Mancuso P, Peters-Golden M. Leukotrienes enhance the bactericidal activity of alveolar macrophages against Klebsiella pneumoniae through the activation of NADPH oxidase. Blood [print-electronic]. 2005 Aug 8/1/2005; 106(3): 1067-75. PMID: 15718414, PMCID: PMC1895163, PII: 2004-08-3323, DOI: 10.1182/blood-2004-08-3323, ISSN: 0006-4971.
Aronoff DM, Canetti C, Serezani CH, Luo M, Peters-Golden M. Cutting edge: macrophage inhibition by cyclic AMP (cAMP): differential roles of protein kinase A and exchange protein directly activated by cAMP-1. J. Immunol. 2005 Jan 1/15/2005; 174(2): 595-9. PMID: 15634874, PII: 174/2/595, ISSN: 0022-1767.
Serezani CH, Franco AR, Wajc M, Umada Yokoyama-Yasunaka JK, Wunderlich G, Borges MM, Uliana SR. Evaluation of the murine immune response to Leishmania meta 1 antigen delivered as recombinant protein or DNA vaccine. Vaccine. 2002 Nov 11/1/2002; 20(31-32): 3755-63. PMID: 12399206, PII: S0264410X02003547, ISSN: 0264-410X.
Mehrpouya-Bahrami P, Moriarty AK, De Melo P, Keeter WC, Alakhras NS, Nelson AS, Hoover M, Barrios MS, Nadler JL, Serezani CH, Kaplan MH, Galkina EV. STAT4 is expressed in neutrophils and promotes antimicrobial immunity. JCI Insight. 2021 Jul 7/22/2021; 6(14): PMID: 34138758, PMCID: PMC8410094, PII: e141326, DOI: 10.1172/jci.insight.141326, ISSN: 2379-3708.
Bonyek-Silva I, Machado AFA, Cerqueira-Silva T, Nunes S, Silva Cruz MR, Silva J, Santos RL, Barral A, Oliveira PRS, Khouri R, Serezani CH, Brodskyn C, Caldas JR, Barral-Netto M, Boaventura V, Tavares NM. LTB4-Driven Inflammation and Increased Expression of ALOX5/ACE2 During Severe COVID-19 in Individuals With Diabetes. Diabetes [print-electronic]. 2021 Jun 6/15/2021; PMID: 34417262, PII: db20-1260, DOI: 10.2337/db20-1260, ISSN: 1939-327X.
Lyu H, Elkins CM, Pierce JL, Serezani CH, Perrien DS. MyD88 Is Not Required for Muscle Injury-Induced Endochondral Heterotopic Ossification in a Mouse Model of Fibrodysplasia Ossificans Progressiva. Biomedicines. 2021 Jun 6/1/2021; 9(6): PMID: 34206078, PMCID: PMC8227787, PII: biomedicines9060630, DOI: 10.3390/biomedicines9060630, ISSN: 2227-9059.
Klopfenstein N, Cassat JE, Monteith A, Miller A, Drury S, Skaar E, Serezani CH. Murine Models for Staphylococcal Infection. Curr Protoc. 2021 Mar; 1(3): e52. PMID: 33656290, PMCID: PMC7935403, DOI: 10.1002/cpz1.52, ISSN: 2691-1299.
Klopfenstein N, Brandt SL, Castellanos S, Gunzer M, Blackman A, Serezani CH. SOCS-1 inhibition of type I interferon restrains Staphylococcus aureus skin host defense. PLoS Pathog. 2021 Mar; 17(3): e1009387. PMID: 33690673, PMCID: PMC7984627, PII: PPATHOGENS-D-20-02265, DOI: 10.1371/journal.ppat.1009387, ISSN: 1553-7374.
Chastain CA, Klopfenstein N, Serezani CH, Aronoff DM. A Clinical Review of Diabetic Foot Infections. Clin Podiatr Med Surg [print-electronic]. 2019 Jul; 36(3): 381-95. PMID: 31079605, PII: S0891-8422(19)30016-3, DOI: 10.1016/j.cpm.2019.02.004, ISSN: 1558-2302.
Brandt SL, Wang S, Dejani NN, Klopfenstein N, Winfree S, Filgueiras L, McCarthy BP, Territo PR, Serezani CH. Excessive localized leukotriene B4 levels dictate poor skin host defense in diabetic mice. JCI Insight [print-electronic]. 2018 Sep 9/6/2018; 3(17): PMID: 30185672, PII: 120220, DOI: 10.1172/jci.insight.120220, ISSN: 2379-3708.
Dejani NN, Orlando AB, Niño VE, Penteado LA, Verdan FF, Bazzano JMR, Codo AC, Salina ACG, Saraiva AC, Avelar MR, Spolidorio LC, Serezani CH, Medeiros AI. Intestinal host defense outcome is dictated by PGE2 production during efferocytosis of infected cells. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2018 Sep 9/4/2018; 115(36): E8469-E8478. PMID: 30127026, PMCID: PMC6130380, PII: 1722016115, DOI: 10.1073/pnas.1722016115, ISSN: 1091-6490.
Brandt SL, Klopfenstein N, Wang S, Winfree S, McCarthy BP, Territo PR, Miller L, Serezani CH. Macrophage-derived LTB4 promotes abscess formation and clearance of Staphylococcus aureus skin infection in mice. PLoS Pathog. 2018 Aug; 14(8): e1007244. PMID: 30102746, PMCID: PMC6107286, PII: PPATHOGENS-D-18-00195, DOI: 10.1371/journal.ppat.1007244, ISSN: 1553-7374.
Brandt SL, Putnam NE, Cassat JE, Serezani CH. Innate Immunity to Staphylococcus aureus: Evolving Paradigms in Soft Tissue and Invasive Infections. J. Immunol. 2018 Jun 6/15/2018; 200(12): 3871-80. PMID: 29866769, PMCID: PMC6028009, PII: 200/12/3871, DOI: 10.4049/jimmunol.1701574, ISSN: 1550-6606.
Sisti F, Wang S, Brandt SL, Glosson-Byers N, Mayo LD, Son YM, Sturgeon S, Filgueiras L, Jancar S, Wong H, Dela Cruz CS, Andrews N, Alves-Filho JC, Cunha FQ, Serezani CH. Nuclear PTEN enhances the maturation of a microRNA regulon to limit MyD88-dependent susceptibility to sepsis. Sci Signal. 2018 May 5/1/2018; 11(528): PMID: 29717063, PII: 11/528/eaai9085, DOI: 10.1126/scisignal.aai9085, ISSN: 1937-9145.
Braga Filho JAF, Abreu AG, Rios CEP, Trovão LO, Silva DLF, Cysne DN, Nascimento JR, Fortes TS, Silva LA, Guerra RNM, Maciel MCG, Serezani CH, Nascimento FRF. Prophylactic Treatment With Simvastatin Modulates the Immune Response and Increases Animal Survival Following Lethal Sepsis Infection. Front Immunol. 2018; 9: 2137. PMID: 30298072, PMCID: PMC6160584, DOI: 10.3389/fimmu.2018.02137, ISSN: 1664-3224.
Brandt SL, Serezani CH. Too much of a good thing: How modulating LTB4 actions restore host defense in homeostasis or disease. Semin. Immunol. 2017 Oct; 33: 37-43. PMID: 29042027, PMCID: PMC5679129, PII: S1044-5323(16)30079-3, DOI: 10.1016/j.smim.2017.08.006, ISSN: 1096-3618.
Piñeros Alvarez AR, Glosson-Byers N, Brandt S, Wang S, Wong H, Sturgeon S, McCarthy BP, Territo PR, Alves-Filho JC, Serezani CH. SOCS1 is a negative regulator of metabolic reprogramming during sepsis. JCI Insight [print-electronic]. 2017 Jul 7/6/2017; 2(13): PMID: 28679957, PMCID: PMC5499360, PII: 92530, DOI: 10.1172/jci.insight.92530, ISSN: 2379-3708.
Salina AC, Souza TP, Serezani CH, Medeiros AI. Efferocytosis-induced prostaglandin E2 production impairs alveolar macrophage effector functions during Streptococcus pneumoniae infection. Innate Immun [print-electronic]. 2017 Apr; 23(3): 219-27. PMID: 28359217, DOI: 10.1177/1753425916684934, ISSN: 1753-4267.
Dejani NN, Brandt SL, Piñeros A, Glosson-Byers NL, Wang S, Son YM, Medeiros AI, Serezani CH. Topical Prostaglandin E Analog Restores Defective Dendritic Cell-Mediated Th17 Host Defense Against Methicillin-Resistant Staphylococcus Aureus in the Skin of Diabetic Mice. Diabetes [print-electronic]. 2016 Sep 9/7/2016; PMID: 27605625, PII: db16-0565, DOI: 10.2337/db16-0565, ISSN: 1939-327X.
Filgueiras LR, Brandt SL, Ramalho TR, Jancar S, Serezani CH. Imbalance between HDAC and HAT activities drives aberrant STAT1/MyD88 expression in macrophages from type 1 diabetic mice. J. Diabetes Complicat [print-electronic]. 2016 Aug 8/7/2016; PMID: 27623388, PII: S1056-8727(16)30341-5, DOI: 10.1016/j.jdiacomp.2016.08.001, ISSN: 1873-460X.
Li XJ, Deng L, Brandt SL, Goodwin CB, Ma P, Yang Z, Mali RS, Liu Z, Kapur R, Serezani CH, Chan RJ. Role of p85a in neutrophil extra- and intracellular reactive oxygen species generation. Oncotarget. 2016 Apr 4/26/2016; 7(17): 23096-105. PMID: 27049833, PII: 8500, DOI: 10.18632/oncotarget.8500, ISSN: 1949-2553.
Zoccal KF, Sorgi CA, Hori JI, Paula-Silva FW, Arantes EC, Serezani CH, Zamboni DS, Faccioli LH. Opposing roles of LTB4 and PGE2 in regulating the inflammasome-dependent scorpion venom-induced mortality. Nat Commun. 2016; 7: 10760. PMID: 26907476, PMCID: PMC4766425, PII: ncomms10760, DOI: 10.1038/ncomms10760, ISSN: 2041-1723.
Ishizuka EK, Filgueiras LR, Rios FJ, Serezani CH, Jancar S. PAFR activation of NF-¿B p65 or p105 precursor dictates pro- and anti-inflammatory responses during TLR activation in murine macrophages. Sci Rep. 2016; 6: 32092. PMID: 27554194, PMCID: PMC4995467, PII: srep32092, DOI: 10.1038/srep32092, ISSN: 2045-2322.
Carrasco SE, Troxell B, Yang Y, Brandt SL, Li H, Sandusky GE, Condon KW, Serezani CH, Yang XF. Outer surface protein OspC is an antiphagocytic factor that protects Borrelia burgdorferi from phagocytosis by macrophages. Infect. Immun [print-electronic]. 2015 Dec; 83(12): 4848-60. PMID: 26438793, PMCID: PMC4645385, PII: IAI.01215-15, DOI: 10.1128/IAI.01215-15, ISSN: 1098-5522.
Filgueiras LR, Brandt SL, Wang S, Wang Z, Morris DL, Evans-Molina C, Mirmira RG, Jancar S, Serezani CH. Leukotriene B4-mediated sterile inflammation promotes susceptibility to sepsis in a mouse model of type 1 diabetes. Sci Signal. 2015 Jan 1/27/2015; 8(361): ra10. PMID: 25628460, PMCID: PMC4356178, PII: 8/361/ra10, DOI: 10.1126/scisignal.2005568, ISSN: 1937-9145.
Filgueiras LR, Serezani CH, Jancar S. Leukotriene B4 as a Potential Therapeutic Target for the Treatment of Metabolic Disorders. Front Immunol. 2015; 6: 515. PMID: 26500652, PMCID: PMC4597104, DOI: 10.3389/fimmu.2015.00515, ISSN: 1664-3224.
Wang Z, Brandt S, Medeiros A, Wang S, Wu H, Dent A, Serezani CH. MicroRNA 21 is a homeostatic regulator of macrophage polarization and prevents prostaglandin E2-mediated M2 generation. PLoS ONE. 2015; 10(2): e0115855. PMID: 25706647, PMCID: PMC4338261, PII: PONE-D-14-41738, DOI: 10.1371/journal.pone.0115855, ISSN: 1932-6203.
Ribeiro LR, Loures FV, de Araújo EF, Feriotti C, Costa TA, Serezani CH, Jancar S, Calich VL. Lipoxin Inhibits Fungal Uptake by Macrophages and Reduces the Severity of Acute Pulmonary Infection Caused by Paracoccidioides brasiliensis. Mediators Inflamm [print-electronic]. 2015; 2015: 852574. PMID: 26635449, PMCID: PMC4618125, DOI: 10.1155/2015/852574, ISSN: 1466-1861.
Morato CI, da Silva IA, Borges AF, Dorta ML, Oliveira MA, Jancar S, Serezani CH, Ribeiro-Dias F. Essential role of leukotriene B4 on Leishmania (Viannia) braziliensis killing by human macrophages. Microbes Infect [print-electronic]. 2014 Nov; 16(11): 945-53. PMID: 25195516, PII: S1286-4579(14)00134-8, DOI: 10.1016/j.micinf.2014.08.015, ISSN: 1769-714X.
da Silva-Souza HA, Lira MN, Costa-Junior HM, da Cruz CM, Vasconcellos JS, Mendes AN, Pimenta-Reis G, Alvarez CL, Faccioli LH, Serezani CH, Schachter J, Persechini PM. Inhibitors of the 5-lipoxygenase arachidonic acid pathway induce ATP release and ATP-dependent organic cation transport in macrophages. Biochim. Biophys. Acta [print-electronic]. 2014 Jul; 1838(7): 1967-77. PMID: 24743022, PII: S0005-2736(14)00141-2, DOI: 10.1016/j.bbamem.2014.04.006, ISSN: 0006-3002.
Ferreira AE, Sisti F, Sônego F, Wang S, Filgueiras LR, Brandt S, Serezani AP, Du H, Cunha FQ, Alves-Filho JC, Serezani CH. PPAR-¿/IL-10 axis inhibits MyD88 expression and ameliorates murine polymicrobial sepsis. J. Immunol [print-electronic]. 2014 Mar 3/1/2014; 192(5): 2357-65. PMID: 24489087, PMCID: PMC3943997, PII: jimmunol.1302375, DOI: 10.4049/jimmunol.1302375, ISSN: 1550-6606.
Wang Z, Filgueiras LR, Wang S, Serezani AP, Peters-Golden M, Jancar S, Serezani CH. Leukotriene B4 enhances the generation of proinflammatory microRNAs to promote MyD88-dependent macrophage activation. J. Immunol [print-electronic]. 2014 Mar 3/1/2014; 192(5): 2349-56. PMID: 24477912, PMCID: PMC3943984, PII: jimmunol.1302982, DOI: 10.4049/jimmunol.1302982, ISSN: 1550-6606.
Secatto A, Soares EM, Locachevic GA, Assis PA, Paula-Silva FW, Serezani CH, de Medeiros AI, Faccioli LH. The leukotriene B4/BLT1 axis is a key determinant in susceptibility and resistance to histoplasmosis. PLoS ONE. 2014; 9(1): e85083. PMID: 24465479, PMCID: PMC3897419, PII: PONE-D-13-25916, DOI: 10.1371/journal.pone.0085083, ISSN: 1932-6203.
Rogers LM, Thelen T, Fordyce K, Bourdonnay E, Lewis C, Yu H, Zhang J, Xie J, Serezani CH, Peters-Golden M, Aronoff DM. EP4 and EP2 receptor activation of protein kinase A by prostaglandin E2 impairs macrophage phagocytosis of Clostridium sordellii. Am. J. Reprod. Immunol [print-electronic]. 2014 Jan; 71(1): 34-43. PMID: 23902376, PMCID: PMC3864121, DOI: 10.1111/aji.12153, ISSN: 1600-0897.
Domingo-Gonzalez R, Katz S, Serezani CH, Moore TA, Levine AM, Moore BB. Prostaglandin E2-induced changes in alveolar macrophage scavenger receptor profiles differentially alter phagocytosis of Pseudomonas aeruginosa and Staphylococcus aureus post-bone marrow transplant. J. Immunol [print-electronic]. 2013 Jun 6/1/2013; 190(11): 5809-17. PMID: 23630358, PMCID: PMC3660503, PII: jimmunol.1203274, DOI: 10.4049/jimmunol.1203274, ISSN: 1550-6606.
Hoggatt J, Mohammad KS, Singh P, Hoggatt AF, Chitteti BR, Speth JM, Hu P, Poteat BA, Stilger KN, Ferraro F, Silberstein L, Wong FK, Farag SS, Czader M, Milne GL, Breyer RM, Serezani CH, Scadden DT, Guise TA, Srour EF, Pelus LM. Differential stem- and progenitor-cell trafficking by prostaglandin E2. Nature [print-electronic]. 2013 Mar 3/21/2013; 495(7441): 365-9. PMID: 23485965, PMCID: PMC3606692, PII: nature11929, DOI: 10.1038/nature11929, ISSN: 1476-4687.
Soares EM, Mason KL, Rogers LM, Serezani CH, Faccioli LH, Aronoff DM. Leukotriene B4 enhances innate immune defense against the puerperal sepsis agent Streptococcus pyogenes. J. Immunol [print-electronic]. 2013 Feb 2/15/2013; 190(4): 1614-22. PMID: 23325886, PMCID: PMC3563855, PII: jimmunol.1202932, DOI: 10.4049/jimmunol.1202932, ISSN: 1550-6606.
Simões RL, Arruda MA, Canetti C, Serezani CH, Fierro IM, Barja-Fidalgo C. Proinflammatory responses of heme in alveolar macrophages: repercussion in lung hemorrhagic episodes. Mediators Inflamm [print-electronic]. 2013; 2013: 946878. PMID: 23690673, PMCID: PMC3652176, DOI: 10.1155/2013/946878, ISSN: 1466-1861.
Mor-Vaknin N, Legendre M, Yu Y, Serezani CH, Garg SK, Jatzek A, Swanson MD, Gonzalez-Hernandez MJ, Teitz-Tennenbaum S, Punturieri A, Engleberg NC, Banerjee R, Peters-Golden M, Kao JY, Markovitz DM. Murine colitis is mediated by vimentin. Sci Rep [print-electronic]. 2013; 3: 1045. PMID: 23304436, PMCID: PMC3540396, DOI: 10.1038/srep01045, ISSN: 2045-2322.
Serezani CH, Kane S, Collins L, Morato-Marques M, Osterholzer JJ, Peters-Golden M. Macrophage dectin-1 expression is controlled by leukotriene B4 via a GM-CSF/PU.1 axis. J. Immunol [print-electronic]. 2012 Jul 7/15/2012; 189(2): 906-15. PMID: 22696442, PMCID: PMC3392366, PII: jimmunol.1200257, DOI: 10.4049/jimmunol.1200257, ISSN: 1550-6606.
Mancuso P, Myers MG, Goel D, Serezani CH, O'Brien E, Goldberg J, Aronoff DM, Peters-Golden M. Ablation of leptin receptor-mediated ERK activation impairs host defense against Gram-negative pneumonia. J. Immunol [print-electronic]. 2012 Jul 7/15/2012; 189(2): 867-75. PMID: 22685316, PMCID: PMC3392451, PII: jimmunol.1200465, DOI: 10.4049/jimmunol.1200465, ISSN: 1550-6606.
Bourdonnay E, Serezani CH, Aronoff DM, Peters-Golden M. Regulation of alveolar macrophage p40phox: hierarchy of activating kinases and their inhibition by PGE2. J. Leukoc. Biol [print-electronic]. 2012 Jul; 92(1): 219-31. PMID: 22544939, PMCID: PMC3382311, PII: jlb.1211590, DOI: 10.1189/jlb.1211590, ISSN: 1938-3673.
Zaslona Z, Serezani CH, Okunishi K, Aronoff DM, Peters-Golden M. Prostaglandin E2 restrains macrophage maturation via E prostanoid receptor 2/protein kinase A signaling. Blood [print-electronic]. 2012 Mar 3/8/2012; 119(10): 2358-67. PMID: 22234697, PMCID: PMC3311259, PII: blood-2011-08-374207, DOI: 10.1182/blood-2011-08-374207, ISSN: 1528-0020.
Serezani CH, Kane S, Medeiros AI, Cornett AM, Kim SH, Marques MM, Lee SP, Lewis C, Bourdonnay E, Ballinger MN, White ES, Peters-Golden M. PTEN directly activates the actin depolymerization factor cofilin-1 during PGE2-mediated inhibition of phagocytosis of fungi. Sci Signal. 2012 Feb 2/7/2012; 5(210): ra12. PMID: 22317922, PMCID: PMC3400468, PII: 5/210/ra12, DOI: 10.1126/scisignal.2002448, ISSN: 1937-9145.
Filgueiras LR, Martins JO, Serezani CH, Capelozzi VL, Montes MB, Jancar S. Sepsis-induced acute lung injury (ALI) is milder in diabetic rats and correlates with impaired NFkB activation. PLoS ONE [print-electronic]. 2012; 7(9): e44987. PMID: 23024779, PMCID: PMC3443211, PII: PONE-D-12-14042, DOI: 10.1371/journal.pone.0044987, ISSN: 1932-6203.
Medeiros A, Peres-Buzalaf C, Fortino Verdan F, Serezani CH. Prostaglandin E2 and the suppression of phagocyte innate immune responses in different organs. Mediators Inflamm [print-electronic]. 2012; 2012: 327568. PMID: 23024463, PMCID: PMC3449139, DOI: 10.1155/2012/327568, ISSN: 1466-1861.
Secatto A, Rodrigues LC, Serezani CH, Ramos SG, Dias-Baruffi M, Faccioli LH, Medeiros AI. 5-Lipoxygenase deficiency impairs innate and adaptive immune responses during fungal infection. PLoS ONE [print-electronic]. 2012; 7(3): e31701. PMID: 22448213, PMCID: PMC3308949, PII: PONE-D-11-09055, DOI: 10.1371/journal.pone.0031701, ISSN: 1932-6203.
Morato-Marques M, Campos MR, Kane S, Rangel AP, Lewis C, Ballinger MN, Kim SH, Peters-Golden M, Jancar S, Serezani CH. Leukotrienes target F-actin/cofilin-1 to enhance alveolar macrophage anti-fungal activity. J. Biol. Chem [print-electronic]. 2011 Aug 8/19/2011; 286(33): 28902-13. PMID: 21715328, PMCID: PMC3190697, PII: M111.235309, DOI: 10.1074/jbc.M111.235309, ISSN: 1083-351X.
Kim SH, Serezani CH, Okunishi K, Zaslona Z, Aronoff DM, Peters-Golden M. Distinct protein kinase A anchoring proteins direct prostaglandin E2 modulation of Toll-like receptor signaling in alveolar macrophages. J. Biol. Chem [print-electronic]. 2011 Mar 3/18/2011; 286(11): 8875-83. PMID: 21247892, PMCID: PMC3058957, PII: M110.187815, DOI: 10.1074/jbc.M110.187815, ISSN: 1083-351X.
Serezani CH, Lewis C, Jancar S, Peters-Golden M. Leukotriene B4 amplifies NF-¿B activation in mouse macrophages by reducing SOCS1 inhibition of MyD88 expression. J. Clin. Invest [print-electronic]. 2011 Feb; 121(2): 671-82. PMID: 21206089, PMCID: PMC3026722, PII: 43302, DOI: 10.1172/JCI43302, ISSN: 1558-8238.
Thelen T, Hao Y, Medeiros AI, Curtis JL, Serezani CH, Kobzik L, Harris LH, Aronoff DM. The class A scavenger receptor, macrophage receptor with collagenous structure, is the major phagocytic receptor for Clostridium sordellii expressed by human decidual macrophages. J. Immunol [print-electronic]. 2010 Oct 10/1/2010; 185(7): 4328-35. PMID: 20810988, PII: jimmunol.1000989, DOI: 10.4049/jimmunol.1000989, ISSN: 1550-6606.
Mancuso P, Lewis C, Serezani CH, Goel D, Peters-Golden M. Intrapulmonary administration of leukotriene B4 enhances pulmonary host defense against pneumococcal pneumonia. Infect. Immun [print-electronic]. 2010 May; 78(5): 2264-71. PMID: 20231413, PMCID: PMC2863513, PII: IAI.01323-09, DOI: 10.1128/IAI.01323-09, ISSN: 1098-5522.
Serezani CH, Aronoff DM, Sitrin RG, Peters-Golden M. FcgammaRI ligation leads to a complex with BLT1 in lipid rafts that enhances rat lung macrophage antimicrobial functions. Blood [print-electronic]. 2009 Oct 10/8/2009; 114(15): 3316-24. PMID: 19657115, PMCID: PMC2759654, PII: blood-2009-01-199919, DOI: 10.1182/blood-2009-01-199919, ISSN: 1528-0020.
Aronoff DM, Lewis C, Serezani CH, Eaton KA, Goel D, Phipps JC, Peters-Golden M, Mancuso P. E-prostanoid 3 receptor deletion improves pulmonary host defense and protects mice from death in severe Streptococcus pneumoniae infection. J. Immunol [print-electronic]. 2009 Aug 8/15/2009; 183(4): 2642-9. PMID: 19635910, PMCID: PMC4016992, PII: jimmunol.0900129, DOI: 10.4049/jimmunol.0900129, ISSN: 1550-6606.
Campos MR, Serezani CH, Peters-Golden M, Jancar S. Differential kinase requirement for enhancement of Fc gammaR-mediated phagocytosis in alveolar macrophages by leukotriene B4 vs. D4. Mol. Immunol [print-electronic]. 2009 Mar; 46(6): 1204-11. PMID: 19223078, PII: S0161-5890(08)00791-8, DOI: 10.1016/j.molimm.2008.11.024, ISSN: 1872-9142.
Medeiros AI, Serezani CH, Lee SP, Peters-Golden M. Efferocytosis impairs pulmonary macrophage and lung antibacterial function via PGE2/EP2 signaling. J. Exp. Med [print-electronic]. 2009 Jan 1/16/2009; 206(1): 61-8. PMID: 19124657, PMCID: PMC2626688, PII: jem.20082058, DOI: 10.1084/jem.20082058, ISSN: 1540-9538.
Lee SP, Serezani CH, Medeiros AI, Ballinger MN, Peters-Golden M. Crosstalk between prostaglandin E2 and leukotriene B4 regulates phagocytosis in alveolar macrophages via combinatorial effects on cyclic AMP. J. Immunol. 2009 Jan 1/1/2009; 182(1): 530-7. PMID: 19109185, PII: 182/1/530, ISSN: 1550-6606.
Costa-Junior HM, Mendes AN, Davis GH, da Cruz CM, Ventura AL, Serezani CH, Faccioli LH, Nomizo A, Freire-de-Lima CG, Bisaggio Rda C, Persechini PM. ATP-induced apoptosis involves a Ca2+-independent phospholipase A2 and 5-lipoxygenase in macrophages. Prostaglandins Other Lipid Mediat [print-electronic]. 2009 Jan; 88(1-2): 51-61. PMID: 18984060, PII: S1098-8823(08)00067-1, DOI: 10.1016/j.prostaglandins.2008.09.004, ISSN: 1098-8823.
Medeiros AI, Sá-Nunes A, Turato WM, Secatto A, Frantz FG, Sorgi CA, Serezani CH, Deepe GS, Faccioli LH. Leukotrienes are potent adjuvant during fungal infection: effects on memory T cells. J. Immunol. 2008 Dec 12/15/2008; 181(12): 8544-51. PMID: 19050273, PII: 181/12/8544, ISSN: 1550-6606.
Weinlich R, Bortoluci KR, Chehab CF, Serezani CH, Ulbrich AG, Peters-Golden M, Russo M, Amarante-Mendes GP. TLR4/MYD88-dependent, LPS-induced synthesis of PGE2 by macrophages or dendritic cells prevents anti-CD3-mediated CD95L upregulation in T cells. Cell Death Differ [print-electronic]. 2008 Dec; 15(12): 1901-9. PMID: 18820644, PII: cdd2008128, DOI: 10.1038/cdd.2008.128, ISSN: 1476-5403.
Chung J, Serezani CH, Huang SK, Stern JN, Keskin DB, Jagirdar R, Brock TG, Aronoff DM, Peters-Golden M. Rap1 activation is required for Fc gamma receptor-dependent phagocytosis. J. Immunol. 2008 Oct 10/15/2008; 181(8): 5501-9. PMID: 18832707, PMCID: PMC3077557, PII: 181/8/5501, ISSN: 1550-6606.
Serezani CH, Ballinger MN, Aronoff DM, Peters-Golden M. Cyclic AMP: master regulator of innate immune cell function. Am. J. Respir. Cell Mol. Biol [print-electronic]. 2008 Aug; 39(2): 127-32. PMID: 18323530, PMCID: PMC2720142, PII: 2008-0091TR, DOI: 10.1165/rcmb.2008-0091TR, ISSN: 1535-4989.
Aronoff DM, Hao Y, Chung J, Coleman N, Lewis C, Peres CM, Serezani CH, Chen GH, Flamand N, Brock TG, Peters-Golden M. Misoprostol impairs female reproductive tract innate immunity against Clostridium sordellii. J. Immunol. 2008 Jun 6/15/2008; 180(12): 8222-30. PMID: 18523288, PMCID: PMC2667109, PII: 180/12/8222, ISSN: 0022-1767.
Brock TG, Serezani CH, Carstens JK, Peters-Golden M, Aronoff DM. Effects of prostaglandin E2 on the subcellular localization of Epac-1 and Rap1 proteins during Fcgamma-receptor-mediated phagocytosis in alveolar macrophages. Exp. Cell Res [print-electronic]. 2008 Jan 1/15/2008; 314(2): 255-63. PMID: 18021770, PMCID: PMC2390918, PII: S0014-4827(07)00491-0, DOI: 10.1016/j.yexcr.2007.10.011, ISSN: 0014-4827.
Canetti C, Serezani CH, Atrasz RG, White ES, Aronoff DM, Peters-Golden M. Activation of phosphatase and tensin homolog on chromosome 10 mediates the inhibition of FcgammaR phagocytosis by prostaglandin E2 in alveolar macrophages. J. Immunol. 2007 Dec 12/15/2007; 179(12): 8350-6. PMID: 18056380, PII: 179/12/8350, ISSN: 0022-1767.
Coffey MJ, Serezani CH, Phare SM, Flamand N, Peters-Golden M. NADPH oxidase deficiency results in reduced alveolar macrophage 5-lipoxygenase expression and decreased leukotriene synthesis. J. Leukoc. Biol [print-electronic]. 2007 Dec; 82(6): 1585-91. PMID: 17761955, PII: jlb.0107019, DOI: 10.1189/jlb.0107019, ISSN: 0741-5400.
Serezani CH, Chung J, Ballinger MN, Moore BB, Aronoff DM, Peters-Golden M. Prostaglandin E2 suppresses bacterial killing in alveolar macrophages by inhibiting NADPH oxidase. Am. J. Respir. Cell Mol. Biol [print-electronic]. 2007 Nov; 37(5): 562-70. PMID: 17585108, PMCID: PMC2048683, PII: 2007-0153OC, DOI: 10.1165/rcmb.2007-0153OC, ISSN: 1535-4989.
Peres CM, Aronoff DM, Serezani CH, Flamand N, Faccioli LH, Peters-Golden M. Specific leukotriene receptors couple to distinct G proteins to effect stimulation of alveolar macrophage host defense functions. J. Immunol. 2007 Oct 10/15/2007; 179(8): 5454-61. PMID: 17911632, PII: 179/8/5454, ISSN: 0022-1767.
Flamand N, Mancuso P, Serezani CH, Brock TG. Leukotrienes: mediators that have been typecast as villains. Cell. Mol. Life Sci. 2007 Oct; 64(19-20): 2657-70. PMID: 17639273, DOI: 10.1007/s00018-007-7228-2, ISSN: 1420-682X.
Aronoff DM, Peres CM, Serezani CH, Ballinger MN, Carstens JK, Coleman N, Moore BB, Peebles RS, Faccioli LH, Peters-Golden M. Synthetic prostacyclin analogs differentially regulate macrophage function via distinct analog-receptor binding specificities. J. Immunol. 2007 Feb 2/1/2007; 178(3): 1628-34. PMID: 17237412, PII: 178/3/1628, ISSN: 0022-1767.
Aronoff DM, Serezani CH, Carstens JK, Marshall T, Gangireddy SR, Peters-Golden M, Reddy RC. Stimulatory Effects of Peroxisome Proliferator-Activated Receptor-gamma on Fcgamma Receptor-Mediated Phagocytosis by Alveolar Macrophages. PPAR Res. 2007; 2007: 52546. PMID: 18253476, PMCID: PMC2220046, DOI: 10.1155/2007/52546, ISSN: 1687-4757.
Serezani CH, Perrela JH, Russo M, Peters-Golden M, Jancar S. Leukotrienes are essential for the control of Leishmania amazonensis infection and contribute to strain variation in susceptibility. J. Immunol. 2006 Sep 9/1/2006; 177(5): 3201-8. PMID: 16920959, PII: 177/5/3201, ISSN: 0022-1767.
Ballinger MN, Paine R, Serezani CH, Aronoff DM, Choi ES, Standiford TJ, Toews GB, Moore BB. Role of granulocyte macrophage colony-stimulating factor during gram-negative lung infection with Pseudomonas aeruginosa. Am. J. Respir. Cell Mol. Biol [print-electronic]. 2006 Jun; 34(6): 766-74. PMID: 16474098, PMCID: PMC2644237, PII: 2005-0246OC, DOI: 10.1165/rcmb.2005-0246OC, ISSN: 1044-1549.
Serezani CH, Aronoff DM, Jancar S, Peters-Golden M. Leukotriene B4 mediates p47phox phosphorylation and membrane translocation in polyunsaturated fatty acid-stimulated neutrophils. J. Leukoc. Biol [print-electronic]. 2005 Oct; 78(4): 976-84. PMID: 16006535, PII: jlb.1004587, DOI: 10.1189/jlb.1004587, ISSN: 0741-5400.
Serezani CH, Aronoff DM, Jancar S, Mancuso P, Peters-Golden M. Leukotrienes enhance the bactericidal activity of alveolar macrophages against Klebsiella pneumoniae through the activation of NADPH oxidase. Blood [print-electronic]. 2005 Aug 8/1/2005; 106(3): 1067-75. PMID: 15718414, PMCID: PMC1895163, PII: 2004-08-3323, DOI: 10.1182/blood-2004-08-3323, ISSN: 0006-4971.
Aronoff DM, Canetti C, Serezani CH, Luo M, Peters-Golden M. Cutting edge: macrophage inhibition by cyclic AMP (cAMP): differential roles of protein kinase A and exchange protein directly activated by cAMP-1. J. Immunol. 2005 Jan 1/15/2005; 174(2): 595-9. PMID: 15634874, PII: 174/2/595, ISSN: 0022-1767.
Serezani CH, Franco AR, Wajc M, Umada Yokoyama-Yasunaka JK, Wunderlich G, Borges MM, Uliana SR. Evaluation of the murine immune response to Leishmania meta 1 antigen delivered as recombinant protein or DNA vaccine. Vaccine. 2002 Nov 11/1/2002; 20(31-32): 3755-63. PMID: 12399206, PII: S0264410X02003547, ISSN: 0264-410X.
Available Postdoctoral Position Details
Posted: 1/7/2021
The Serezani lab at Vanderbilt University Medical Center is looking to recruit a postdoctoral associate to work on different NIH-funded projects. Dr. Serezani is also a member of many institutional NIH T32 Training Grant that could provide multi-year support for postdoctoral trainees (USA citizens and Permanent Residents).
Our lab is interested in understanding how phagocyte's intracellular brakes influence host defense in systemic and localized infections. More specifically, we want to investigate:
1) Host-derived factors that account for enhanced susceptibility to infections in preexisting conditions. Using the in vivo imaging system (IVIS) and intravital microscopy, we are dissecting the dynamics of different monocyte, macrophage, and neutrophil populations in the skin and lung. We have been investigating how ingestion of dead cells (efferocytosis) influences metabolic reprogramming in diabetes, obesity, and aging. Another lab project aims to assess the role of microRNAs (mainly miR21) in neutrophil function during skin host defense.
2) Identification and actions of pleiotropic intracellular inhibitors during sepsis. We have been interested in identifying intracellular events that could decrease systemic inflammatory response during sepsis. We have been focusing on understanding the cellular and molecular targets of phosphatases (PTEN and SHP1) and suppressor of cytokine signaling (SOCS) in neutrophils and macrophages during sepsis. We are identifying new regulatory programs triggered by PTEN and SOCS1 in inflammasome assembly and fatty acid oxidation that influences organ damage and mortality in sepsis.
The projects will be tailored to meet the individual qualifications and interests of the candidate. For example, the nature and extent of advanced microscopy and bioinformatics or a focus on different animal infection models can be discussed with the applicant.
Qualifications • Suitable applicants should have a recent Ph.D. in immunology or a related field, a record of productivity, and excellent communication skills. • The ideal candidate will work well in an interactive scientific environment, be intellectually curious, and have experience in all aspects of immunology. • Research experience in immunology, cell biology, and/or microbiology • Critical skills include¿extensive experience with animal handling, hands-on experience with, and extensive knowledge of tissue culture (including isolation of primary cells), basic molecular biology techniques, and basic fluorescent microscopy • Proven track record of troubleshooting and problem-solving. • Strong organizational skills with the ability to balance multiple projects in a fast-paced environment and bring projects to completion. • Willingness to learning bioinformatics to perform metabolomics, lipidomics, and RNAseq analysis • Interested in learning advanced microscopy, such as super-resolution microscopy, high-resolution live-cell imaging, and intravital microscopy. • Proven track record of troubleshooting and problem-solving. • Strong organizational skills with the ability to balance multiple projects in a fast-paced environment and bring projects to completion. • Helpful Nature – Offers to help lighten the load for others in times of need; volunteers rather than waiting to be asked; understands that almost no one works in a silo and needs the help of others to do their job well; recognizes that by helping others first, others will likely reciprocate Principal Responsibilities • Spearhead projects investigating the role of phosphatases in the metabolism control of epigenetics and inflammatory response • Independently design and conduct experiments under the supervision of the Principal Investigator. • Conduct literature reviews to be used in determining the most suitable research methods. • Present data in lab meetings (~1 x month),
Candidate must be available to start working immediately or a later start date can be discussed. Highly motivated individuals should submit a single PDF file containing CV, a brief description of research interests and experience, and the names of 2-3 references to h.serezan@vumc.org