Faculty Appointments
Assistant Professor of Biochemistry
Education
Ph.D., Yeast Genetics, Newcastle University, Newcastle upon Tyne, United KingdomM.A., Molecular and Celluar Biology, University of Bath, Bath, United Kingdom
Office Address
Vanderbilt University
2215 Garland Ave
619 Light Hall
Nashville, TN 37232
2215 Garland Ave
619 Light Hall
Nashville, TN 37232
Research Description
For cellular life to exist, genetic material must be copied and passed on to newly divided cells. In humans, this process is phenomenally accurate and occurs with an error rate of around one in a billion. In the Dewar lab, we are working to understand the mechanisms that underlie the completion of DNA synthesis, which poses unique problems that are not present during earlier stages of replication. Our research is important to better-understand molecular causes of aging and cancer, develop better cancer treatments, and evaluate the risk of genotoxins (both environmental and endogenous).
A complete list of Dr Dewar's publications can be found here: https://www.ncbi.nlm.nih.gov/myncbi/1hS1ghNWDPW5c/bibliography/public/
A complete list of Dr Dewar's publications can be found here: https://www.ncbi.nlm.nih.gov/myncbi/1hS1ghNWDPW5c/bibliography/public/
Research Keywords
Cancer, cancer biology, chemotherapy, chemotherapeutics, aging, ageing, cell cycle, S phase, nucleus, molecular biology, biochemistry, mass spectrometry, proteomics, DNA, DNA replication, genome, genome duplication, replication termination, replication fork, topoisomerase, topoisomerases, replisome, telomere, telomeres, telomerase, Shelterin, genome stability, genotoxin, toxicology, environmental toxicology, environmental toxin.
Publications
Campos LV, Van Ravenstein SX, Vontalge EJ, Greer BH, Heintzman DR, Kavlashvili T, McDonald WH, Rose KL, Eichman BF, Dewar JM. RTEL1 and MCM10 overcome topological stress during vertebrate replication termination. Cell Rep [print-electronic]. 2023 Feb 2/17/2023; 42(2): 112109. PMID: 36807139, PMCID: PMC10432576, PII: S2211-1247(23)00120-1, DOI: 10.1016/j.celrep.2023.112109, ISSN: 2211-1247.
Kavlashvili T, Liu W, Mohamed TM, Cortez D, Dewar JM. Replication fork uncoupling causes nascent strand degradation and fork reversal. Nat Struct Mol Biol [print-electronic]. 2023 Jan; 30(1): 115-24. PMID: 36593312, PMCID: PMC9868089, PII: 10.1038/s41594-022-00871-y, DOI: 10.1038/s41594-022-00871-y, ISSN: 1545-9985.
Heintzman DR, Campos LV, Byl JAW, Osheroff N, Dewar JM. Topoisomerase II Is Crucial for Fork Convergence during Vertebrate Replication Termination. Cell Rep. 2019 Oct 10/8/2019; 29(2): 422-436.e5. PMID: 31597101, PMCID: PMC6919565, PII: S2211-1247(19)31159-3, DOI: 10.1016/j.celrep.2019.08.097, ISSN: 2211-1247.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D. Initiation of DNA replication requires actin dynamics and formin activity. EMBO J [print-electronic]. 2017 Nov 11/2/2017; 36(21): 3212-31. PMID: 28982779, PMCID: PMC5666611, PII: embj.201796585, DOI: 10.15252/embj.201796585, ISSN: 1460-2075.
Dewar JM, Walter JC. Mechanisms of DNA replication termination. Nat Rev Mol Cell Biol. 2017 Aug; 18(8): 507-16. PMID: 28537574.
Dewar JM, Low E, Mann M, Raschle M, Walter JC. CRL2Lrr1 promotes unloading of the vertebrate replisome from chromatin during replication termination. Genes Dev. 2017 Feb 2/1/2017; 31(3): 275-90. PMID: 28235849.
Dewar JM, Budzowska M, Walter JC. The mechanism of DNA replication termination in vertebrates. Nature [print-electronic]. 2015 Sep 9/17/2015; 525(7569): 345-50. PMID: 26322582, PMCID: PMC4575634, PII: nature14887, DOI: 10.1038/nature14887, ISSN: 1476-4687.
Zhang J, Dewar JM, Budzowska M, Motnenko A, Cohn MA, Walter JC. DNA interstrand cross-link repair requires replication-fork convergence. Nat. Struct. Mol. Biol [print-electronic]. 2015 Mar; 22(3): 242-7. PMID: 25643322, PMCID: PMC4351167, PII: nsmb.2956, DOI: 10.1038/nsmb.2956, ISSN: 1545-9985.
Duxin JP, Dewar JM, Yardimci H, Walter JC. Repair of a DNA-protein crosslink by replication-coupled proteolysis. Cell. 2014 Oct 10/9/2014; 159(2): 346-57. PMID: 25303529, PMCID: PMC4229047, PII: S0092-8674(14)01173-8, DOI: 10.1016/j.cell.2014.09.024, ISSN: 1097-4172.
Dewar JM, Walter JC. Chromosome biology: conflict management for replication and transcription. Curr. Biol. 2013 Mar 3/4/2013; 23(5): R200-2. PMID: 23473562, PII: S0960-9822(13)00089-4, DOI: 10.1016/j.cub.2013.01.054, ISSN: 1879-0445.
Dewar JM, Lydall D. Similarities and differences between "uncapped" telomeres and DNA double-strand breaks. Chromosoma [print-electronic]. 2012 Apr; 121(2): 117-30. PMID: 22203190, DOI: 10.1007/s00412-011-0357-2, ISSN: 1432-0886.
Dewar JM, Lydall D. Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast. Methods Mol. Biol. 2012; 920: 341-8. PMID: 22941615, DOI: 10.1007/978-1-61779-998-3_24, ISSN: 1940-6029.
Weile J, Pocock M, Cockell SJ, Lord P, Dewar JM, Holstein EM, Wilkinson D, Lydall D, Hallinan J, Wipat A. Customizable views on semantically integrated networks for systems biology. Bioinformatics [print-electronic]. 2011 May 5/1/2011; 27(9): 1299-306. PMID: 21414991, PMCID: PMC3077072, PII: btr134, DOI: 10.1093/bioinformatics/btr134, ISSN: 1367-4811.
Dewar JM, Lydall D. Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncapping. EMBO J [print-electronic]. 2010 Dec 12/1/2010; 29(23): 4020-34. PMID: 21045806, PMCID: PMC3020640, PII: emboj2010267, DOI: 10.1038/emboj.2010.267, ISSN: 1460-2075.
Dewar JM, Lydall D. Telomere replication: Mre11 leads the way. Mol. Cell. 2010 Jun 6/25/2010; 38(6): 777-9. PMID: 20620949, PII: S1097-2765(10)00444-2, DOI: 10.1016/j.molcel.2010.06.003, ISSN: 1097-4164.
Addinall SG, Downey M, Yu M, Zubko MK, Dewar J, Leake A, Hallinan J, Shaw O, James K, Wilkinson DJ, Wipat A, Durocher D, Lydall D. A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae. Genetics [print-electronic]. 2008 Dec; 180(4): 2251-66. PMID: 18845848, PMCID: PMC2600956, PII: genetics.108.092577, DOI: 10.1534/genetics.108.092577, ISSN: 0016-6731.
Kavlashvili T, Liu W, Mohamed TM, Cortez D, Dewar JM. Replication fork uncoupling causes nascent strand degradation and fork reversal. Nat Struct Mol Biol [print-electronic]. 2023 Jan; 30(1): 115-24. PMID: 36593312, PMCID: PMC9868089, PII: 10.1038/s41594-022-00871-y, DOI: 10.1038/s41594-022-00871-y, ISSN: 1545-9985.
Heintzman DR, Campos LV, Byl JAW, Osheroff N, Dewar JM. Topoisomerase II Is Crucial for Fork Convergence during Vertebrate Replication Termination. Cell Rep. 2019 Oct 10/8/2019; 29(2): 422-436.e5. PMID: 31597101, PMCID: PMC6919565, PII: S2211-1247(19)31159-3, DOI: 10.1016/j.celrep.2019.08.097, ISSN: 2211-1247.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D. Initiation of DNA replication requires actin dynamics and formin activity. EMBO J [print-electronic]. 2017 Nov 11/2/2017; 36(21): 3212-31. PMID: 28982779, PMCID: PMC5666611, PII: embj.201796585, DOI: 10.15252/embj.201796585, ISSN: 1460-2075.
Dewar JM, Walter JC. Mechanisms of DNA replication termination. Nat Rev Mol Cell Biol. 2017 Aug; 18(8): 507-16. PMID: 28537574.
Dewar JM, Low E, Mann M, Raschle M, Walter JC. CRL2Lrr1 promotes unloading of the vertebrate replisome from chromatin during replication termination. Genes Dev. 2017 Feb 2/1/2017; 31(3): 275-90. PMID: 28235849.
Dewar JM, Budzowska M, Walter JC. The mechanism of DNA replication termination in vertebrates. Nature [print-electronic]. 2015 Sep 9/17/2015; 525(7569): 345-50. PMID: 26322582, PMCID: PMC4575634, PII: nature14887, DOI: 10.1038/nature14887, ISSN: 1476-4687.
Zhang J, Dewar JM, Budzowska M, Motnenko A, Cohn MA, Walter JC. DNA interstrand cross-link repair requires replication-fork convergence. Nat. Struct. Mol. Biol [print-electronic]. 2015 Mar; 22(3): 242-7. PMID: 25643322, PMCID: PMC4351167, PII: nsmb.2956, DOI: 10.1038/nsmb.2956, ISSN: 1545-9985.
Duxin JP, Dewar JM, Yardimci H, Walter JC. Repair of a DNA-protein crosslink by replication-coupled proteolysis. Cell. 2014 Oct 10/9/2014; 159(2): 346-57. PMID: 25303529, PMCID: PMC4229047, PII: S0092-8674(14)01173-8, DOI: 10.1016/j.cell.2014.09.024, ISSN: 1097-4172.
Dewar JM, Walter JC. Chromosome biology: conflict management for replication and transcription. Curr. Biol. 2013 Mar 3/4/2013; 23(5): R200-2. PMID: 23473562, PII: S0960-9822(13)00089-4, DOI: 10.1016/j.cub.2013.01.054, ISSN: 1879-0445.
Dewar JM, Lydall D. Similarities and differences between "uncapped" telomeres and DNA double-strand breaks. Chromosoma [print-electronic]. 2012 Apr; 121(2): 117-30. PMID: 22203190, DOI: 10.1007/s00412-011-0357-2, ISSN: 1432-0886.
Dewar JM, Lydall D. Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast. Methods Mol. Biol. 2012; 920: 341-8. PMID: 22941615, DOI: 10.1007/978-1-61779-998-3_24, ISSN: 1940-6029.
Weile J, Pocock M, Cockell SJ, Lord P, Dewar JM, Holstein EM, Wilkinson D, Lydall D, Hallinan J, Wipat A. Customizable views on semantically integrated networks for systems biology. Bioinformatics [print-electronic]. 2011 May 5/1/2011; 27(9): 1299-306. PMID: 21414991, PMCID: PMC3077072, PII: btr134, DOI: 10.1093/bioinformatics/btr134, ISSN: 1367-4811.
Dewar JM, Lydall D. Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncapping. EMBO J [print-electronic]. 2010 Dec 12/1/2010; 29(23): 4020-34. PMID: 21045806, PMCID: PMC3020640, PII: emboj2010267, DOI: 10.1038/emboj.2010.267, ISSN: 1460-2075.
Dewar JM, Lydall D. Telomere replication: Mre11 leads the way. Mol. Cell. 2010 Jun 6/25/2010; 38(6): 777-9. PMID: 20620949, PII: S1097-2765(10)00444-2, DOI: 10.1016/j.molcel.2010.06.003, ISSN: 1097-4164.
Addinall SG, Downey M, Yu M, Zubko MK, Dewar J, Leake A, Hallinan J, Shaw O, James K, Wilkinson DJ, Wipat A, Durocher D, Lydall D. A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae. Genetics [print-electronic]. 2008 Dec; 180(4): 2251-66. PMID: 18845848, PMCID: PMC2600956, PII: genetics.108.092577, DOI: 10.1534/genetics.108.092577, ISSN: 0016-6731.