Heather K. Kroh, Ph.D.

Research Assistant Professor

Faculty Appointments
Research Assistant Professor of Pathology, Microbiology and Immunology
B.S., Biology, Tennessee Technological University, Cookeville, TennesseePh.D., Vanderbilt University, Nashville, Tennessee
Maddur AA, Kroh HK, Aschenbrenner ME, Gibson BHY, Panizzi P, Sheehan JH, Meiler J, Bock PE, Verhamme IM. Specificity and affinity of the N-terminal residues in staphylocoagulase in binding to prothrombin. J Biol Chem [print-electronic]. 2020 Apr 4/24/2020; 295(17): 5614-25. PMID: 32156702, PMCID: PMC7186164, PII: S0021-9258(17)50292-6, DOI: 10.1074/jbc.RA120.012588, ISSN: 1083-351X.

Tam J, Icho S, Utama E, Orrell KE, Gómez-Biagi RF, Theriot CM, Kroh HK, Rutherford SA, Lacy DB, Melnyk RA. Intestinal bile acids directly modulate the structure and function of C. difficile TcdB toxin. Proc Natl Acad Sci U S A [print-electronic]. 2020 Mar 3/24/2020; 117(12): 6792-800. PMID: 32152097, PMCID: PMC7104382, PII: 1916965117, DOI: 10.1073/pnas.1916965117, ISSN: 1091-6490.

Kroh HK, Chandrasekaran R, Zhang Z, Rosenthal K, Woods R, Jin X, Nyborg AC, Rainey GJ, Warrener P, Melnyk RA, Spiller BW, Lacy DB. A neutralizing antibody that blocks delivery of the enzymatic cargo of Clostridium difficile toxin TcdB into host cells. J. Biol. Chem [print-electronic]. 2018 Jan 1/19/2018; 293(3): 941-52. PMID: 29180448, PMCID: PMC5777265, PII: M117.813428, DOI: 10.1074/jbc.M117.813428, ISSN: 1083-351X.

Gupta P, Zhang Z, Sugiman-Marangos SN, Tam J, Raman S, Julien JP, Kroh HK, Lacy DB, Murgolo N, Bekkari K, Therien AG, Hernandez LD, Melnyk RA. Functional defects in Clostridium difficile TcdB toxin uptake identify CSPG4 receptor-binding determinants. J. Biol. Chem [print-electronic]. 2017 Oct 10/20/2017; 292(42): 17290-301. PMID: 28842504, PMCID: PMC5655507, PII: M117.806687, DOI: 10.1074/jbc.M117.806687, ISSN: 1083-351X.

Kroh HK, Chandrasekaran R, Rosenthal K, Woods R, Jin X, Ohi MD, Nyborg AC, Rainey GJ, Warrener P, Spiller BW, Lacy DB. Use of a neutralizing antibody helps identify structural features critical for binding of Clostridium difficile toxin TcdA to the host cell surface. J. Biol. Chem [print-electronic]. 2017 Sep 9/1/2017; 292(35): 14401-12. PMID: 28705932, PMCID: PMC5582835, PII: M117.781112, DOI: 10.1074/jbc.M117.781112, ISSN: 1083-351X.

Hernandez LD, Kroh HK, Hsieh E, Yang X, Beaumont M, Sheth PR, DiNunzio E, Rutherford SA, Ohi MD, Ermakov G, Xiao L, Secore S, Karczewski J, Racine F, Mayhood T, Fischer P, Sher X, Gupta P, Lacy DB, Therien AG. Epitopes and Mechanism of Action of the Clostridium difficile Toxin A-Neutralizing Antibody Actoxumab. J. Mol. Biol [print-electronic]. 2017 Apr 4/7/2017; 429(7): 1030-44. PMID: 28232034, PII: S0022-2836(17)30085-2, DOI: 10.1016/j.jmb.2017.02.010, ISSN: 1089-8638.

Quesada-Gómez C, López-Ureña D, Chumbler N, Kroh HK, Castro-Peña C, Rodríguez C, Orozco-Aguilar J, González-Camacho S, Rucavado A, Guzmán-Verri C, Lawley TD, Lacy DB, Chaves-Olarte E. Analysis of TcdB Proteins within the Hypervirulent Clade 2 Reveals an Impact of RhoA Glucosylation on Clostridium difficile Proinflammatory Activities. Infect. Immun. 2016 Jan 1/11/2016; 84(3): 856-65. PMID: 26755157, PMCID: PMC4771349, PII: IAI.01291-15, DOI: 10.1128/IAI.01291-15, ISSN: 1098-5522.

Kroh HK, Bock PE. Effect of zymogen domains and active site occupation on activation of prothrombin by von Willebrand factor-binding protein. J. Biol. Chem [print-electronic]. 2012 Nov 11/9/2012; 287(46): 39149-57. PMID: 23012355, PMCID: PMC3493955, PII: M112.415562, DOI: 10.1074/jbc.M112.415562, ISSN: 1083-351X.

Kroh HK, Panizzi P, Tchaikovski S, Baird TR, Wei N, Krishnaswamy S, Tans G, Rosing J, Furie B, Furie BC, Bock PE. Active site-labeled prothrombin inhibits prothrombinase in vitro and thrombosis in vivo. J Biol Chem [print-electronic]. 2011 Jul 7/1/2011; 286(26): 23345-56. PMID: 21531712, PMCID: PMC3123099, PII: S0021-9258(19)48869-8, DOI: 10.1074/jbc.M111.230292, ISSN: 1083-351X.

Kroh HK, Panizzi P, Bock PE. Von Willebrand factor-binding protein is a hysteretic conformational activator of prothrombin. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2009 May 5/12/2009; 106(19): 7786-91. PMID: 19416890, PMCID: PMC2683071, PII: 0811750106, DOI: 10.1073/pnas.0811750106, ISSN: 1091-6490.