Faculty Appointments
Associate Professor of Chemistry
Assistant Professor of Pathology, Microbiology and ImmunologyAssistant Professor of Pathology, Microbiology and ImmunologyAssociate Professor of Biological SciencesAssociate Professor of Biological Sciences
Education
Ph.D., Molecular and Cell Biology, University of California, Berkeley, CaliforniaB.S., Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts
Research Description
The focus of the group is to define the dynamics and the coordination of protein interaction networks in diverse cellular processes. Towards this goal, we develop new mass spectrometry-based proteomics and chemical biology tools. Dynamic protein-protein interactions govern many molecular, cellular, and organismal processes and altered interactions are intricately linked to disease states. For instance, in cancer disparate protein-protein interactions occur in signal transduction pathways, protein folding diseases arise from aberrant engagement of protein folding pathways, and pathogenic infections take advantage of host-pathogen protein interactions that co-opt cellular pathways. Our goal is to understand how protein interactions in the pertinent biological processes have to be properly timed and coordinated. Defining the pathological consequences of mistimed and uncoordinated protein interactions on disease states will then guide the identification of new therapeutic strategies.
The research in our group leverages multidisciplinary approaches at the interface of Chemistry and Biology, ranging from protein biochemistry and enzymology, to microbiology, and cell biology as far as proteomics and drug discovery.
We currently work on two interrelated projects:
1) Dynamics of chaperone interactions with client proteins during folding and secretion
2) Coordination of host pathogen protein-protein interactions during flavivirus infections
The research in our group leverages multidisciplinary approaches at the interface of Chemistry and Biology, ranging from protein biochemistry and enzymology, to microbiology, and cell biology as far as proteomics and drug discovery.
We currently work on two interrelated projects:
1) Dynamics of chaperone interactions with client proteins during folding and secretion
2) Coordination of host pathogen protein-protein interactions during flavivirus infections
Research Keywords
Dynamics and coordination of protein interaction networks; proteostasis, protein folding, and protein quality control; host-virus protein interactions; antivirals; chemical biology and proteomics tools; mass spectrometry; biochemistry; enzymology; microbiology
Publications
Cabral-Miranda F, Tamburini G, Martinez G, Ardiles AO, Medinas DB, Gerakis Y, Hung MD, Vidal R, Fuentealba M, Miedema T, Duran-Aniotz C, Diaz J, Ibaceta-Gonzalez C, Sabusap CM, Bermedo-Garcia F, Mujica P, Adamson S, Vitangcol K, Huerta H, Zhang X, Nakamura T, Sardi SP, Lipton SA, Kennedy BK, Henriquez JP, Cárdenas JC, Plate L, Palacios AG, Hetz C. Unfolded protein response IRE1/XBP1 signaling is required for healthy mammalian brain aging. EMBO J [print-electronic]. 2022 Oct 10/31/2022; e111952. PMID: 36314651, DOI: 10.15252/embj.2022111952, ISSN: 1460-2075.
Sanders AL, Hermanson JN, Samuels DC, Plate L, Sanders CR. Compendium of proteins containing segments that exhibit zero-tolerance to amino acid variation in humans. Protein Sci. 2022 Sep; 31(9): e4408. PMID: 36040257, PMCID: PMC9387208, DOI: 10.1002/pro.4408, ISSN: 1469-896X.
McDonald EF, Sabusap CMP, Kim M, Plate L. Distinct proteostasis states drive pharmacologic chaperone susceptibility for cystic fibrosis transmembrane conductance regulator misfolding mutants. Mol Biol Cell [print-electronic]. 2022 Jun 6/1/2022; 33(7): ar62. PMID: 35389766, PMCID: PMC9561855, DOI: 10.1091/mbc.E21-11-0578, ISSN: 1939-4586.
Munden A, Wright MT, Han D, Tirgar R, Plate L, Nordman JT. Identification of replication fork-associated proteins in Drosophila embryos and cultured cells using iPOND coupled to quantitative mass spectrometry. Sci Rep. 2022 Apr 4/28/2022; 12(1): 6903. PMID: 35484306, PMCID: PMC9050644, PII: 10.1038/s41598-022-10821-9, DOI: 10.1038/s41598-022-10821-9, ISSN: 2045-2322.
McDonald EF, Woods H, Smith ST, Kim M, Schoeder CT, Plate L, Meiler J. Structural Comparative Modeling of Multi-Domain F508del CFTR. Biomolecules. 2022 Mar 3/18/2022; 12(3): PMID: 35327663, PMCID: PMC8946492, PII: biom12030471, DOI: 10.3390/biom12030471, ISSN: 2218-273X.
Bilches Medinas D, Malik S, Yildiz-Bölükbasi E, Borgonovo J, Saaranen MJ, Urra H, Pulgar E, Afzal M, Contreras D, Wright MT, Bodaleo F, Quiroz G, Rozas P, Mumtaz S, Díaz R, Rozas C, Cabral-Miranda F, Piña R, Valenzuela V, Uyan O, Reardon C, Woehlbier U, Brown RH, Sena-Esteves M, Gonzalez-Billault C, Morales B, Plate L, Ruddock LW, Concha ML, Hetz C, Tolun A. Mutation in protein disulfide isomerase A3 causes neurodevelopmental defects by disturbing endoplasmic reticulum proteostasis. EMBO J [print-electronic]. 2021 Dec 12/14/2021; e105531. PMID: 34904718, DOI: 10.15252/embj.2020105531, ISSN: 1460-2075.
Reisman BJ, Guo H, Ramsey HE, Wright MT, Reinfeld BI, Ferrell PB, Sulikowski GA, Rathmell WK, Savona MR, Plate L, Rubinstein JL, Bachmann BO. Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase. Nat Chem Biol [print-electronic]. 2021 Dec 12/2/2021; PMID: 34857958, PII: 10.1038/s41589-021-00900-9, DOI: 10.1038/s41589-021-00900-9, ISSN: 1552-4469.
Almasy KM, Davies JP, Plate L. Comparative Host Interactomes of the SARS-CoV-2 Nonstructural Protein 3 and Human Coronavirus Homologs. Mol Cell Proteomics [print-electronic]. 2021 Jun 6/27/2021; 20: 100120. PMID: 34186245, PMCID: PMC8236078, PII: S1535-9476(21)00092-X, DOI: 10.1016/j.mcpro.2021.100120, ISSN: 1535-9484.
Almasy KM, Davies JP, Plate L. Comparative host interactomes of the SARS-CoV-2 nonstructural protein 3 and human coronavirus homologs. BioRxiv. 2021 Mar 3/8/2021; PMID: 33758849, PMCID: PMC7987008, DOI: 10.1101/2021.03.08.434440.
Rozas P, Pinto C, Martínez Traub F, Díaz R, Pérez V, Becerra D, Ojeda P, Ojeda J, Wright MT, Mella J, Plate L, Henríquez JP, Hetz C, Medinas DB. Protein disulfide isomerase ERp57 protects early muscle denervation in experimental ALS. Acta Neuropathol Commun. 2021 Feb 2/4/2021; 9(1): 21. PMID: 33541434, PMCID: PMC7863244, PII: 10.1186/s40478-020-01116-z, DOI: 10.1186/s40478-020-01116-z, ISSN: 2051-5960.
Vidal RL, Sepulveda D, Troncoso-Escudero P, Garcia-Huerta P, Gonzalez C, Plate L, Jerez C, Canovas J, Rivera CA, Castillo V, Cisternas M, Leal S, Martinez A, Grandjean J, Sonia D, Lashuel HA, Martin AJM, Latapiat V, Matus S, Sardi SP, Wiseman RL, Hetz C. Enforced dimerization between XBP1s and ATF6f enhances the protective effects of the UPR in models of neurodegeneration. Mol Ther [print-electronic]. 2021 Feb 2/3/2021; PMID: 33545358, PII: S1525-0016(21)00067-8, DOI: 10.1016/j.ymthe.2021.01.033, ISSN: 1525-0024.
Wright MT, Plate L. Revealing functional insights into ER proteostasis through proteomics and interactomics. Exp Cell Res [print-electronic]. 2021 Feb 2/1/2021; 399(1): 112417. PMID: 33301765, PII: S0014-4827(20)30670-4, DOI: 10.1016/j.yexcr.2020.112417, ISSN: 1090-2422.
Almasy KM, Davies JP, Lisy SM, Tirgar R, Tran SC, Plate L. Small-molecule endoplasmic reticulum proteostasis regulator acts as a broad-spectrum inhibitor of dengue and Zika virus infections. Proc Natl Acad Sci U S A. 2021 Jan 1/19/2021; 118(3): PMID: 33441483, PMCID: PMC7826409, PII: 2012209118, DOI: 10.1073/pnas.2012209118, ISSN: 1091-6490.
Marinko JT, Wright MT, Schlebach JP, Clowes KR, Heintzman DR, Plate L, Sanders CR. Glycosylation limits forward trafficking of the tetraspan membrane protein PMP22. J Biol Chem [print-electronic]. 2021 Jan; 296: 100719. PMID: 33933451, PMCID: PMC8191293, PII: S0021-9258(21)00508-1, DOI: 10.1016/j.jbc.2021.100719, ISSN: 1083-351X.
Davies JP, Almasy KM, McDonald EF, Plate L. Comparative Multiplexed Interactomics of SARS-CoV-2 and Homologous Coronavirus Nonstructural Proteins Identifies Unique and Shared Host-Cell Dependencies. ACS Infect Dis [print-electronic]. 2020 Dec 12/11/2020; 6(12): 3174-89. PMID: 33263384, PMCID: PMC7724760, DOI: 10.1021/acsinfecdis.0c00500, ISSN: 2373-8227.
Wright MT, Kouba L, Plate L. Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis. Mol Cell Proteomics [print-electronic]. 2020 Dec 12/8/2020; 20: 100008. PMID: 33581410, PMCID: PMC7950113, PII: S1535-9476(20)35122-7, DOI: 10.1074/mcp.RA120.002168, ISSN: 1535-9484.
Wright MT, Kouba L, Plate L. Thyroglobulin interactome profiling defines altered proteostasis topology associated with thyroid dyshormonogenesis. Mol Cell Proteomics [print-electronic]. 2020 Nov 11/18/2020; PMID: 33208410, PII: RA120.002168, DOI: 10.1074/mcp.RA120.002168, ISSN: 1535-9484.
Grandjean JMD, Madhavan A, Cech L, Seguinot BO, Paxman RJ, Smith E, Scampavia L, Powers ET, Cooley CB, Plate L, Spicer TP, Kelly JW, Wiseman RL. Pharmacologic IRE1/XBP1s activation confers targeted ER proteostasis reprogramming. Nature chemical biology [print-electronic]. 2020 Oct; 16(10): 1052-61. PMID: 32690944, PMCID: PMC7502540, PII: 10.1038/s41589-020-0584-z, DOI: 10.1038/s41589-020-0584-z, ISSN: 1552-4469.
Davies JP, Almasy KM, McDonald EF, Plate L. Comparative multiplexed interactomics of SARS-CoV-2 and homologous coronavirus non-structural proteins identifies unique and shared host-cell dependencies. BioRxiv. 2020 Jul 7/14/2020; PMID: 32699849, PMCID: PMC7373130, DOI: 10.1101/2020.07.13.201517.
García-Huerta P, Troncoso-Escudero P, Wu D, Thiruvalluvan A, Cisternas-Olmedo M, Henríquez DR, Plate L, Chana-Cuevas P, Saquel C, Thielen P, Longo KA, Geddes BJ, Lederkremer GZ, Sharma N, Shenkman M, Naphade S, Sardi SP, Spichiger C, Richter HG, Court FA, Tshilenge KT, Ellerby LM, Wiseman RL, Gonzalez-Billault C, Bergink S, Vidal RL, Hetz C. Insulin-like growth factor 2 (IGF2) protects against Huntington's disease through the extracellular disposal of protein aggregates. Acta Neuropathol [print-electronic]. 2020 Jul 7/8/2020; PMID: 32642868, PII: 10.1007/s00401-020-02183-1, DOI: 10.1007/s00401-020-02183-1, ISSN: 1432-0533.
Plate L, Rius B, Nguyen B, Genereux JC, Kelly JW, Wiseman RL. Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein. Cell Chem Biol [print-electronic]. 2019 Jul 7/18/2019; 26(7): 913-925.e4. PMID: 31105062, PMCID: PMC6642020, PII: S2451-9456(19)30115-1, DOI: 10.1016/j.chembiol.2019.04.001, ISSN: 2451-9448.
Torres SE, Gallagher CM, Plate L, Gupta M, Liem CR, Guo X, Tian R, Stroud RM, Kampmann M, Weissman JS, Walter P. Ceapins block the unfolded protein response sensor ATF6a by inducing a neomorphic inter-organelle tether. Elife. 2019 May 5/31/2019; 8: PMID: 31149896, PMCID: PMC6588346, PII: 46595, DOI: 10.7554/eLife.46595, ISSN: 2050-084X.
Grandjean JMD, Plate L, Morimoto RI, Bollong MJ, Powers ET, Wiseman RL. Deconvoluting Stress-Responsive Proteostasis Signaling Pathways for Pharmacologic Activation Using Targeted RNA Sequencing. ACS Chem. Biol [print-electronic]. 2019 Mar 3/13/2019; PMID: 30821953, DOI: 10.1021/acschembio.9b00134, ISSN: 1554-8937.
Blackwood EA, Azizi K, Thuerauf DJ, Paxman RJ, Plate L, Kelly JW, Wiseman RL, Glembotski CC. Pharmacologic ATF6 activation confers global protection in widespread disease models by reprograming cellular proteostasis. Nat Commun. 2019 Jan 1/14/2019; 10(1): 187. PMID: 30643122, PMCID: PMC6331617, PII: 10.1038/s41467-018-08129-2, DOI: 10.1038/s41467-018-08129-2, ISSN: 2041-1723.
Paxman R, Plate L, Blackwood EA, Glembotski C, Powers ET, Wiseman RL, Kelly JW. Pharmacologic ATF6 activating compounds are metabolically activated to selectively modify endoplasmic reticulum proteins. Elife. 2018 Aug 8/7/2018; 7: PMID: 30084354, PMCID: PMC6080950, PII: 37168, DOI: 10.7554/eLife.37168, ISSN: 2050-084X.
Kroeger H, Grimsey N, Paxman R, Chiang WC, Plate L, Jones Y, Shaw PX, Trejo J, Tsang SH, Powers E, Kelly JW, Wiseman RL, Lin JH. The unfolded protein response regulator ATF6 promotes mesodermal differentiation. Sci Signal. 2018 Feb 2/13/2018; 11(517): PMID: 29440509, PII: 11/517/eaan5785, DOI: 10.1126/scisignal.aan5785, ISSN: 1937-9145.
Mortenson DE, Brighty GJ, Plate L, Bare G, Chen W, Li S, Wang H, Cravatt BF, Forli S, Powers ET, Sharpless KB, Wilson IA, Kelly JW. "Inverse Drug Discovery" Strategy To Identify Proteins That Are Targeted by Latent Electrophiles As Exemplified by Aryl Fluorosulfates. J. Am. Chem. Soc [print-electronic]. 2018 Jan 1/10/2018; 140(1): 200-10. PMID: 29265822, PMCID: PMC5762408, DOI: 10.1021/jacs.7b08366, ISSN: 1520-5126.
Schonhoft JD, Monteiro C, Plate L, Eisele YS, Kelly JM, Boland D, Parker CG, Cravatt BF, Teruya S, Helmke S, Maurer M, Berk J, Sekijima Y, Novais M, Coelho T, Powers ET, Kelly JW. Peptide probes detect misfolded transthyretin oligomers in plasma of hereditary amyloidosis patients. Sci Transl Med. 2017 Sep 9/13/2017; 9(407): PMID: 28904227, PMCID: PMC5628019, PII: 9/407/eaam7621, DOI: 10.1126/scitranslmed.aam7621, ISSN: 1946-6242.
Chen KC, Qu S, Chowdhury S, Noxon IC, Schonhoft JD, Plate L, Powers ET, Kelly JW, Lander GC, Wiseman RL. The endoplasmic reticulum HSP40 co-chaperone ERdj3/DNAJB11 assembles and functions as a tetramer. EMBO J [print-electronic]. 2017 Aug 8/1/2017; 36(15): 2296-309. PMID: 28655754, PMCID: PMC5538767, PII: embj.201695616, DOI: 10.15252/embj.201695616, ISSN: 1460-2075.
Alvarez-Garcia O, Matsuzaki T, Olmer M, Plate L, Kelly JW, Lotz MK. Regulated in Development and DNA Damage Response 1 Deficiency Impairs Autophagy and Mitochondrial Biogenesis in Articular Cartilage and Increases the Severity of Experimental Osteoarthritis. Arthritis & rheumatology (Hoboken, N.J.) [print-electronic]. 2017 Jul; 69(7): 1418-28. PMID: 28334504, PMCID: PMC5489357, DOI: 10.1002/art.40104, ISSN: 2326-5205.
Plate L, Wiseman RL. Regulating Secretory Proteostasis through the Unfolded Protein Response: From Function to Therapy. Trends Cell Biol [print-electronic]. 2017 Jun 6/21/2017; PMID: 28647092, PII: S0962-8924(17)30085-5, DOI: 10.1016/j.tcb.2017.05.006, ISSN: 1879-3088.
Plate L, Cooley CB, Chen JJ, Paxman RJ, Gallagher CM, Madoux F, Genereux JC, Dobbs W, Garza D, Spicer TP, Scampavia L, Brown SJ, Rosen H, Powers ET, Walter P, Hodder P, Wiseman RL, Kelly JW. Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation. Elife. 2016 Jul 7/20/2016; 5: PMID: 27435961, PMCID: PMC4954754, DOI: 10.7554/eLife.15550, ISSN: 2050-084X.
Plate L, Paxman RJ, Wiseman RL, Kelly JW. Modulating protein quality control. Elife. 2016 Jul 7/20/2016; 5: PMID: 27435959, PMCID: PMC4954752, DOI: 10.7554/eLife.18431, ISSN: 2050-084X.
Chen W, Dong J, Plate L, Mortenson DE, Brighty GJ, Li S, Liu Y, Galmozzi A, Lee PS, Hulce JJ, Cravatt BF, Saez E, Powers ET, Wilson IA, Sharpless KB, Kelly JW. Arylfluorosulfates Inactivate Intracellular Lipid Binding Protein(s) through Chemoselective SuFEx Reaction with a Binding Site Tyr Residue. J. Am. Chem. Soc [print-electronic]. 2016 Jun 6/15/2016; 138(23): 7353-64. PMID: 27191344, PMCID: PMC4909538, DOI: 10.1021/jacs.6b02960, ISSN: 1520-5126.
Cooley CB, Ryno LM, Plate L, Morgan GJ, Hulleman JD, Kelly JW, Wiseman RL. Unfolded protein response activation reduces secretion and extracellular aggregation of amyloidogenic immunoglobulin light chain. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2014 Sep 9/9/2014; 111(36): 13046-51. PMID: 25157167, PMCID: PMC4246986, PII: 1406050111, DOI: 10.1073/pnas.1406050111, ISSN: 1091-6490.
Plate L, Marletta MA. Phosphorylation-dependent derepression by the response regulator HnoC in the Shewanella oneidensis nitric oxide signaling network. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Nov 11/26/2013; 110(48): E4648-57. PMID: 24218564, PMCID: PMC3845109, PII: 1318128110, DOI: 10.1073/pnas.1318128110, ISSN: 1091-6490.
Plate L, Marletta MA. Nitric oxide-sensing H-NOX proteins govern bacterial communal behavior. Trends Biochem. Sci [print-electronic]. 2013 Nov; 38(11): 566-75. PMID: 24113192, PMCID: PMC3819143, PII: S0968-0004(13)00154-0, DOI: 10.1016/j.tibs.2013.08.008, ISSN: 0968-0004.
Ivanisevic J, Zhu ZJ, Plate L, Tautenhahn R, Chen S, O'Brien PJ, Johnson CH, Marletta MA, Patti GJ, Siuzdak G. Toward 'omic scale metabolite profiling: a dual separation-mass spectrometry approach for coverage of lipid and central carbon metabolism. Anal. Chem [print-electronic]. 2013 Jul 7/16/2013; 85(14): 6876-84. PMID: 23781873, PMCID: PMC3761963, DOI: 10.1021/ac401140h, ISSN: 1520-6882.
Plate L, Marletta MA. Nitric oxide modulates bacterial biofilm formation through a multicomponent cyclic-di-GMP signaling network. Mol. Cell [print-electronic]. 2012 May 5/25/2012; 46(4): 449-60. PMID: 22542454, PMCID: PMC3361614, PII: S1097-2765(12)00260-2, DOI: 10.1016/j.molcel.2012.03.023, ISSN: 1097-4164.
Carlson HK, Plate L, Price MS, Allen JJ, Shokat KM, Marletta MA. Use of a semisynthetic epitope to probe histidine kinase activity and regulation. Anal. Biochem [print-electronic]. 2010 Feb 2/15/2010; 397(2): 139-43. PMID: 19819215, PMCID: PMC2812668, PII: S0003-2697(09)00709-X, DOI: 10.1016/j.ab.2009.10.009, ISSN: 1096-0309.
Weinert EE, Plate L, Whited CA, Olea C, Marletta MA. Determinants of ligand affinity and heme reactivity in H-NOX domains. Angew. Chem. Int. Ed. Engl. 2010; 49(4): 720-3. PMID: 20017169, PMCID: PMC3517115, DOI: 10.1002/anie.200904799, ISSN: 1521-3773.
Hassan AQ, Wang Y, Plate L, Stubbe J. Methodology to probe subunit interactions in ribonucleotide reductases. Biochemistry. 2008 Dec 12/9/2008; 47(49): 13046-55. PMID: 19012414, PMCID: PMC2645937, DOI: 10.1021/bi8012559, ISSN: 1520-4995.
Gianolio DA, Philbrook M, Avila LZ, Young LE, Plate L, Santos MR, Bernasconi R, Liu H, Ahn S, Sun W, Jarrett PK, Miller RJ. Hyaluronan-tethered opioid depots: synthetic strategies and release kinetics in vitro and in vivo. Bioconjug. Chem [print-electronic]. 2008 Sep; 19(9): 1767-74. PMID: 18717537, DOI: 10.1021/bc8000479, ISSN: 1520-4812.
Sanders AL, Hermanson JN, Samuels DC, Plate L, Sanders CR. Compendium of proteins containing segments that exhibit zero-tolerance to amino acid variation in humans. Protein Sci. 2022 Sep; 31(9): e4408. PMID: 36040257, PMCID: PMC9387208, DOI: 10.1002/pro.4408, ISSN: 1469-896X.
McDonald EF, Sabusap CMP, Kim M, Plate L. Distinct proteostasis states drive pharmacologic chaperone susceptibility for cystic fibrosis transmembrane conductance regulator misfolding mutants. Mol Biol Cell [print-electronic]. 2022 Jun 6/1/2022; 33(7): ar62. PMID: 35389766, PMCID: PMC9561855, DOI: 10.1091/mbc.E21-11-0578, ISSN: 1939-4586.
Munden A, Wright MT, Han D, Tirgar R, Plate L, Nordman JT. Identification of replication fork-associated proteins in Drosophila embryos and cultured cells using iPOND coupled to quantitative mass spectrometry. Sci Rep. 2022 Apr 4/28/2022; 12(1): 6903. PMID: 35484306, PMCID: PMC9050644, PII: 10.1038/s41598-022-10821-9, DOI: 10.1038/s41598-022-10821-9, ISSN: 2045-2322.
McDonald EF, Woods H, Smith ST, Kim M, Schoeder CT, Plate L, Meiler J. Structural Comparative Modeling of Multi-Domain F508del CFTR. Biomolecules. 2022 Mar 3/18/2022; 12(3): PMID: 35327663, PMCID: PMC8946492, PII: biom12030471, DOI: 10.3390/biom12030471, ISSN: 2218-273X.
Bilches Medinas D, Malik S, Yildiz-Bölükbasi E, Borgonovo J, Saaranen MJ, Urra H, Pulgar E, Afzal M, Contreras D, Wright MT, Bodaleo F, Quiroz G, Rozas P, Mumtaz S, Díaz R, Rozas C, Cabral-Miranda F, Piña R, Valenzuela V, Uyan O, Reardon C, Woehlbier U, Brown RH, Sena-Esteves M, Gonzalez-Billault C, Morales B, Plate L, Ruddock LW, Concha ML, Hetz C, Tolun A. Mutation in protein disulfide isomerase A3 causes neurodevelopmental defects by disturbing endoplasmic reticulum proteostasis. EMBO J [print-electronic]. 2021 Dec 12/14/2021; e105531. PMID: 34904718, DOI: 10.15252/embj.2020105531, ISSN: 1460-2075.
Reisman BJ, Guo H, Ramsey HE, Wright MT, Reinfeld BI, Ferrell PB, Sulikowski GA, Rathmell WK, Savona MR, Plate L, Rubinstein JL, Bachmann BO. Apoptolidin family glycomacrolides target leukemia through inhibition of ATP synthase. Nat Chem Biol [print-electronic]. 2021 Dec 12/2/2021; PMID: 34857958, PII: 10.1038/s41589-021-00900-9, DOI: 10.1038/s41589-021-00900-9, ISSN: 1552-4469.
Almasy KM, Davies JP, Plate L. Comparative Host Interactomes of the SARS-CoV-2 Nonstructural Protein 3 and Human Coronavirus Homologs. Mol Cell Proteomics [print-electronic]. 2021 Jun 6/27/2021; 20: 100120. PMID: 34186245, PMCID: PMC8236078, PII: S1535-9476(21)00092-X, DOI: 10.1016/j.mcpro.2021.100120, ISSN: 1535-9484.
Almasy KM, Davies JP, Plate L. Comparative host interactomes of the SARS-CoV-2 nonstructural protein 3 and human coronavirus homologs. BioRxiv. 2021 Mar 3/8/2021; PMID: 33758849, PMCID: PMC7987008, DOI: 10.1101/2021.03.08.434440.
Rozas P, Pinto C, Martínez Traub F, Díaz R, Pérez V, Becerra D, Ojeda P, Ojeda J, Wright MT, Mella J, Plate L, Henríquez JP, Hetz C, Medinas DB. Protein disulfide isomerase ERp57 protects early muscle denervation in experimental ALS. Acta Neuropathol Commun. 2021 Feb 2/4/2021; 9(1): 21. PMID: 33541434, PMCID: PMC7863244, PII: 10.1186/s40478-020-01116-z, DOI: 10.1186/s40478-020-01116-z, ISSN: 2051-5960.
Vidal RL, Sepulveda D, Troncoso-Escudero P, Garcia-Huerta P, Gonzalez C, Plate L, Jerez C, Canovas J, Rivera CA, Castillo V, Cisternas M, Leal S, Martinez A, Grandjean J, Sonia D, Lashuel HA, Martin AJM, Latapiat V, Matus S, Sardi SP, Wiseman RL, Hetz C. Enforced dimerization between XBP1s and ATF6f enhances the protective effects of the UPR in models of neurodegeneration. Mol Ther [print-electronic]. 2021 Feb 2/3/2021; PMID: 33545358, PII: S1525-0016(21)00067-8, DOI: 10.1016/j.ymthe.2021.01.033, ISSN: 1525-0024.
Wright MT, Plate L. Revealing functional insights into ER proteostasis through proteomics and interactomics. Exp Cell Res [print-electronic]. 2021 Feb 2/1/2021; 399(1): 112417. PMID: 33301765, PII: S0014-4827(20)30670-4, DOI: 10.1016/j.yexcr.2020.112417, ISSN: 1090-2422.
Almasy KM, Davies JP, Lisy SM, Tirgar R, Tran SC, Plate L. Small-molecule endoplasmic reticulum proteostasis regulator acts as a broad-spectrum inhibitor of dengue and Zika virus infections. Proc Natl Acad Sci U S A. 2021 Jan 1/19/2021; 118(3): PMID: 33441483, PMCID: PMC7826409, PII: 2012209118, DOI: 10.1073/pnas.2012209118, ISSN: 1091-6490.
Marinko JT, Wright MT, Schlebach JP, Clowes KR, Heintzman DR, Plate L, Sanders CR. Glycosylation limits forward trafficking of the tetraspan membrane protein PMP22. J Biol Chem [print-electronic]. 2021 Jan; 296: 100719. PMID: 33933451, PMCID: PMC8191293, PII: S0021-9258(21)00508-1, DOI: 10.1016/j.jbc.2021.100719, ISSN: 1083-351X.
Davies JP, Almasy KM, McDonald EF, Plate L. Comparative Multiplexed Interactomics of SARS-CoV-2 and Homologous Coronavirus Nonstructural Proteins Identifies Unique and Shared Host-Cell Dependencies. ACS Infect Dis [print-electronic]. 2020 Dec 12/11/2020; 6(12): 3174-89. PMID: 33263384, PMCID: PMC7724760, DOI: 10.1021/acsinfecdis.0c00500, ISSN: 2373-8227.
Wright MT, Kouba L, Plate L. Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis. Mol Cell Proteomics [print-electronic]. 2020 Dec 12/8/2020; 20: 100008. PMID: 33581410, PMCID: PMC7950113, PII: S1535-9476(20)35122-7, DOI: 10.1074/mcp.RA120.002168, ISSN: 1535-9484.
Wright MT, Kouba L, Plate L. Thyroglobulin interactome profiling defines altered proteostasis topology associated with thyroid dyshormonogenesis. Mol Cell Proteomics [print-electronic]. 2020 Nov 11/18/2020; PMID: 33208410, PII: RA120.002168, DOI: 10.1074/mcp.RA120.002168, ISSN: 1535-9484.
Grandjean JMD, Madhavan A, Cech L, Seguinot BO, Paxman RJ, Smith E, Scampavia L, Powers ET, Cooley CB, Plate L, Spicer TP, Kelly JW, Wiseman RL. Pharmacologic IRE1/XBP1s activation confers targeted ER proteostasis reprogramming. Nature chemical biology [print-electronic]. 2020 Oct; 16(10): 1052-61. PMID: 32690944, PMCID: PMC7502540, PII: 10.1038/s41589-020-0584-z, DOI: 10.1038/s41589-020-0584-z, ISSN: 1552-4469.
Davies JP, Almasy KM, McDonald EF, Plate L. Comparative multiplexed interactomics of SARS-CoV-2 and homologous coronavirus non-structural proteins identifies unique and shared host-cell dependencies. BioRxiv. 2020 Jul 7/14/2020; PMID: 32699849, PMCID: PMC7373130, DOI: 10.1101/2020.07.13.201517.
García-Huerta P, Troncoso-Escudero P, Wu D, Thiruvalluvan A, Cisternas-Olmedo M, Henríquez DR, Plate L, Chana-Cuevas P, Saquel C, Thielen P, Longo KA, Geddes BJ, Lederkremer GZ, Sharma N, Shenkman M, Naphade S, Sardi SP, Spichiger C, Richter HG, Court FA, Tshilenge KT, Ellerby LM, Wiseman RL, Gonzalez-Billault C, Bergink S, Vidal RL, Hetz C. Insulin-like growth factor 2 (IGF2) protects against Huntington's disease through the extracellular disposal of protein aggregates. Acta Neuropathol [print-electronic]. 2020 Jul 7/8/2020; PMID: 32642868, PII: 10.1007/s00401-020-02183-1, DOI: 10.1007/s00401-020-02183-1, ISSN: 1432-0533.
Plate L, Rius B, Nguyen B, Genereux JC, Kelly JW, Wiseman RL. Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein. Cell Chem Biol [print-electronic]. 2019 Jul 7/18/2019; 26(7): 913-925.e4. PMID: 31105062, PMCID: PMC6642020, PII: S2451-9456(19)30115-1, DOI: 10.1016/j.chembiol.2019.04.001, ISSN: 2451-9448.
Torres SE, Gallagher CM, Plate L, Gupta M, Liem CR, Guo X, Tian R, Stroud RM, Kampmann M, Weissman JS, Walter P. Ceapins block the unfolded protein response sensor ATF6a by inducing a neomorphic inter-organelle tether. Elife. 2019 May 5/31/2019; 8: PMID: 31149896, PMCID: PMC6588346, PII: 46595, DOI: 10.7554/eLife.46595, ISSN: 2050-084X.
Grandjean JMD, Plate L, Morimoto RI, Bollong MJ, Powers ET, Wiseman RL. Deconvoluting Stress-Responsive Proteostasis Signaling Pathways for Pharmacologic Activation Using Targeted RNA Sequencing. ACS Chem. Biol [print-electronic]. 2019 Mar 3/13/2019; PMID: 30821953, DOI: 10.1021/acschembio.9b00134, ISSN: 1554-8937.
Blackwood EA, Azizi K, Thuerauf DJ, Paxman RJ, Plate L, Kelly JW, Wiseman RL, Glembotski CC. Pharmacologic ATF6 activation confers global protection in widespread disease models by reprograming cellular proteostasis. Nat Commun. 2019 Jan 1/14/2019; 10(1): 187. PMID: 30643122, PMCID: PMC6331617, PII: 10.1038/s41467-018-08129-2, DOI: 10.1038/s41467-018-08129-2, ISSN: 2041-1723.
Paxman R, Plate L, Blackwood EA, Glembotski C, Powers ET, Wiseman RL, Kelly JW. Pharmacologic ATF6 activating compounds are metabolically activated to selectively modify endoplasmic reticulum proteins. Elife. 2018 Aug 8/7/2018; 7: PMID: 30084354, PMCID: PMC6080950, PII: 37168, DOI: 10.7554/eLife.37168, ISSN: 2050-084X.
Kroeger H, Grimsey N, Paxman R, Chiang WC, Plate L, Jones Y, Shaw PX, Trejo J, Tsang SH, Powers E, Kelly JW, Wiseman RL, Lin JH. The unfolded protein response regulator ATF6 promotes mesodermal differentiation. Sci Signal. 2018 Feb 2/13/2018; 11(517): PMID: 29440509, PII: 11/517/eaan5785, DOI: 10.1126/scisignal.aan5785, ISSN: 1937-9145.
Mortenson DE, Brighty GJ, Plate L, Bare G, Chen W, Li S, Wang H, Cravatt BF, Forli S, Powers ET, Sharpless KB, Wilson IA, Kelly JW. "Inverse Drug Discovery" Strategy To Identify Proteins That Are Targeted by Latent Electrophiles As Exemplified by Aryl Fluorosulfates. J. Am. Chem. Soc [print-electronic]. 2018 Jan 1/10/2018; 140(1): 200-10. PMID: 29265822, PMCID: PMC5762408, DOI: 10.1021/jacs.7b08366, ISSN: 1520-5126.
Schonhoft JD, Monteiro C, Plate L, Eisele YS, Kelly JM, Boland D, Parker CG, Cravatt BF, Teruya S, Helmke S, Maurer M, Berk J, Sekijima Y, Novais M, Coelho T, Powers ET, Kelly JW. Peptide probes detect misfolded transthyretin oligomers in plasma of hereditary amyloidosis patients. Sci Transl Med. 2017 Sep 9/13/2017; 9(407): PMID: 28904227, PMCID: PMC5628019, PII: 9/407/eaam7621, DOI: 10.1126/scitranslmed.aam7621, ISSN: 1946-6242.
Chen KC, Qu S, Chowdhury S, Noxon IC, Schonhoft JD, Plate L, Powers ET, Kelly JW, Lander GC, Wiseman RL. The endoplasmic reticulum HSP40 co-chaperone ERdj3/DNAJB11 assembles and functions as a tetramer. EMBO J [print-electronic]. 2017 Aug 8/1/2017; 36(15): 2296-309. PMID: 28655754, PMCID: PMC5538767, PII: embj.201695616, DOI: 10.15252/embj.201695616, ISSN: 1460-2075.
Alvarez-Garcia O, Matsuzaki T, Olmer M, Plate L, Kelly JW, Lotz MK. Regulated in Development and DNA Damage Response 1 Deficiency Impairs Autophagy and Mitochondrial Biogenesis in Articular Cartilage and Increases the Severity of Experimental Osteoarthritis. Arthritis & rheumatology (Hoboken, N.J.) [print-electronic]. 2017 Jul; 69(7): 1418-28. PMID: 28334504, PMCID: PMC5489357, DOI: 10.1002/art.40104, ISSN: 2326-5205.
Plate L, Wiseman RL. Regulating Secretory Proteostasis through the Unfolded Protein Response: From Function to Therapy. Trends Cell Biol [print-electronic]. 2017 Jun 6/21/2017; PMID: 28647092, PII: S0962-8924(17)30085-5, DOI: 10.1016/j.tcb.2017.05.006, ISSN: 1879-3088.
Plate L, Cooley CB, Chen JJ, Paxman RJ, Gallagher CM, Madoux F, Genereux JC, Dobbs W, Garza D, Spicer TP, Scampavia L, Brown SJ, Rosen H, Powers ET, Walter P, Hodder P, Wiseman RL, Kelly JW. Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation. Elife. 2016 Jul 7/20/2016; 5: PMID: 27435961, PMCID: PMC4954754, DOI: 10.7554/eLife.15550, ISSN: 2050-084X.
Plate L, Paxman RJ, Wiseman RL, Kelly JW. Modulating protein quality control. Elife. 2016 Jul 7/20/2016; 5: PMID: 27435959, PMCID: PMC4954752, DOI: 10.7554/eLife.18431, ISSN: 2050-084X.
Chen W, Dong J, Plate L, Mortenson DE, Brighty GJ, Li S, Liu Y, Galmozzi A, Lee PS, Hulce JJ, Cravatt BF, Saez E, Powers ET, Wilson IA, Sharpless KB, Kelly JW. Arylfluorosulfates Inactivate Intracellular Lipid Binding Protein(s) through Chemoselective SuFEx Reaction with a Binding Site Tyr Residue. J. Am. Chem. Soc [print-electronic]. 2016 Jun 6/15/2016; 138(23): 7353-64. PMID: 27191344, PMCID: PMC4909538, DOI: 10.1021/jacs.6b02960, ISSN: 1520-5126.
Cooley CB, Ryno LM, Plate L, Morgan GJ, Hulleman JD, Kelly JW, Wiseman RL. Unfolded protein response activation reduces secretion and extracellular aggregation of amyloidogenic immunoglobulin light chain. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2014 Sep 9/9/2014; 111(36): 13046-51. PMID: 25157167, PMCID: PMC4246986, PII: 1406050111, DOI: 10.1073/pnas.1406050111, ISSN: 1091-6490.
Plate L, Marletta MA. Phosphorylation-dependent derepression by the response regulator HnoC in the Shewanella oneidensis nitric oxide signaling network. Proc. Natl. Acad. Sci. U.S.A [print-electronic]. 2013 Nov 11/26/2013; 110(48): E4648-57. PMID: 24218564, PMCID: PMC3845109, PII: 1318128110, DOI: 10.1073/pnas.1318128110, ISSN: 1091-6490.
Plate L, Marletta MA. Nitric oxide-sensing H-NOX proteins govern bacterial communal behavior. Trends Biochem. Sci [print-electronic]. 2013 Nov; 38(11): 566-75. PMID: 24113192, PMCID: PMC3819143, PII: S0968-0004(13)00154-0, DOI: 10.1016/j.tibs.2013.08.008, ISSN: 0968-0004.
Ivanisevic J, Zhu ZJ, Plate L, Tautenhahn R, Chen S, O'Brien PJ, Johnson CH, Marletta MA, Patti GJ, Siuzdak G. Toward 'omic scale metabolite profiling: a dual separation-mass spectrometry approach for coverage of lipid and central carbon metabolism. Anal. Chem [print-electronic]. 2013 Jul 7/16/2013; 85(14): 6876-84. PMID: 23781873, PMCID: PMC3761963, DOI: 10.1021/ac401140h, ISSN: 1520-6882.
Plate L, Marletta MA. Nitric oxide modulates bacterial biofilm formation through a multicomponent cyclic-di-GMP signaling network. Mol. Cell [print-electronic]. 2012 May 5/25/2012; 46(4): 449-60. PMID: 22542454, PMCID: PMC3361614, PII: S1097-2765(12)00260-2, DOI: 10.1016/j.molcel.2012.03.023, ISSN: 1097-4164.
Carlson HK, Plate L, Price MS, Allen JJ, Shokat KM, Marletta MA. Use of a semisynthetic epitope to probe histidine kinase activity and regulation. Anal. Biochem [print-electronic]. 2010 Feb 2/15/2010; 397(2): 139-43. PMID: 19819215, PMCID: PMC2812668, PII: S0003-2697(09)00709-X, DOI: 10.1016/j.ab.2009.10.009, ISSN: 1096-0309.
Weinert EE, Plate L, Whited CA, Olea C, Marletta MA. Determinants of ligand affinity and heme reactivity in H-NOX domains. Angew. Chem. Int. Ed. Engl. 2010; 49(4): 720-3. PMID: 20017169, PMCID: PMC3517115, DOI: 10.1002/anie.200904799, ISSN: 1521-3773.
Hassan AQ, Wang Y, Plate L, Stubbe J. Methodology to probe subunit interactions in ribonucleotide reductases. Biochemistry. 2008 Dec 12/9/2008; 47(49): 13046-55. PMID: 19012414, PMCID: PMC2645937, DOI: 10.1021/bi8012559, ISSN: 1520-4995.
Gianolio DA, Philbrook M, Avila LZ, Young LE, Plate L, Santos MR, Bernasconi R, Liu H, Ahn S, Sun W, Jarrett PK, Miller RJ. Hyaluronan-tethered opioid depots: synthetic strategies and release kinetics in vitro and in vivo. Bioconjug. Chem [print-electronic]. 2008 Sep; 19(9): 1767-74. PMID: 18717537, DOI: 10.1021/bc8000479, ISSN: 1520-4812.