Seesandra V. Rajagopala, Ph.D.

Research Assistant Professor

s.v.rajagopala@vanderbilt.edu
Faculty Appointments
Research Assistant Professor of Medicine
Education
Ph.D., Microbiology, Universität Heidelberg, Heidelberg, GermanyM.Sc., Biology, Bangalore University, Bangalore, India
Publications
Wuchty S, Rajagopala SV, Blazie SM, Parrish JR, Khuri S, Finley RL, Uetz P. The Protein Interactome of Streptococcus pneumoniae and Bacterial Meta-interactomes Improve Function Predictions. MSystems. 2017 May; 2(3): PMID: 28744484, PMCID: PMC5513735, PII: mSystems00019-17, DOI: 10.1128/mSystems.00019-17, ISSN: 2379-5077.

Rajagopala SV, Vashee S, Oldfield LM, Suzuki Y, Venter JC, Telenti A, Nelson KE. The Human Microbiome and Cancer. Cancer Prev Res (Phila) [print-electronic]. 2017 Apr; 10(4): 226-34. PMID: 28096237, PII: 1940-6207.CAPR-16-0249, DOI: 10.1158/1940-6207.CAPR-16-0249, ISSN: 1940-6215.

Singh H, Yu Y, Suh MJ, Torralba MG, Stenzel RD, Tovchigrechko A, Thovarai V, Harkins DM, Rajagopala SV, Osborne W, Cogen FR, Kaplowitz PB, Nelson KE, Madupu R, Pieper R. Type 1 Diabetes: Urinary Proteomics and Protein Network Analysis Support Perturbation of Lysosomal Function. Theranostics. 2017; 7(10): 2704-17. PMID: 28819457, PMCID: PMC5558563, PII: thnov07p2704, DOI: 10.7150/thno.19679, ISSN: 1838-7640.

Rajagopala SV, Yooseph S, Harkins DM, Moncera KJ, Zabokrtsky KB, Torralba MG, Tovchigrechko A, Highlander SK, Pieper R, Sender L, Nelson KE. Gastrointestinal microbial populations can distinguish pediatric and adolescent Acute Lymphoblastic Leukemia (ALL) at the time of disease diagnosis. BMC Genomics. 2016 Aug 8/15/2016; 17(1): 635. PMID: 27527070, PMCID: PMC4986186, PII: 10.1186/s12864-016-2965-y, DOI: 10.1186/s12864-016-2965-y, ISSN: 1471-2164.

Patel MC, Wang W, Pletneva LM, Rajagopala SV, Tan Y, Hartert TV, Boukhvalova MS, Vogel SN, Das SR, Blanco JC. Enterovirus D-68 Infection, Prophylaxis, and Vaccination in a Novel Permissive Animal Model, the Cotton Rat (Sigmodon hispidus). PLoS ONE. 2016; 11(11): e0166336. PMID: 27814404, PMCID: PMC5096705, PII: PONE-D-16-36002, DOI: 10.1371/journal.pone.0166336, ISSN: 1932-6203.

Wallqvist A, Memiševic V, Zavaljevski N, Pieper R, Rajagopala SV, Kwon K, Yu C, Hoover TA, Reifman J. Using host-pathogen protein interactions to identify and characterize Francisella tularensis virulence factors. BMC Genomics. 2015 Dec 12/29/2015; 16: 1106. PMID: 26714771, PMCID: PMC4696196, PII: 10.1186/s12864-015-2351-1, DOI: 10.1186/s12864-015-2351-1, ISSN: 1471-2164.

Memiševic V, Zavaljevski N, Rajagopala SV, Kwon K, Pieper R, DeShazer D, Reifman J, Wallqvist A. Mining host-pathogen protein interactions to characterize Burkholderia mallei infectivity mechanisms. PLoS Comput. Biol. 2015 Mar; 11(3): e1004088. PMID: 25738731, PMCID: PMC4349708, PII: PCOMPBIOL-D-14-01497, DOI: 10.1371/journal.pcbi.1004088, ISSN: 1553-7358.

Rajagopala SV. Mapping the Protein-Protein Interactome Networks Using Yeast Two-Hybrid Screens. Adv. Exp. Med. Biol. 2015; 883: 187-214. PMID: 26621469, DOI: 10.1007/978-3-319-23603-2_11, ISSN: 0065-2598.

Baek JH, Rajagopala SV, Chattoraj DK. Chromosome segregation proteins of Vibrio cholerae as transcription regulators. MBio. 2014 May 5/6/2014; 5(3): e01061-14. PMID: 24803519, PMCID: PMC4010829, PII: mBio.01061-14, DOI: 10.1128/mBio.01061-14, ISSN: 2150-7511.

Häuser R, Ceol A, Rajagopala SV, Mosca R, Siszler G, Wermke N, Sikorski P, Schwarz F, Schick M, Wuchty S, Aloy P, Uetz P. A second-generation protein-protein interaction network of Helicobacter pylori. Mol. Cell Proteomics [print-electronic]. 2014 May; 13(5): 1318-29. PMID: 24627523, PMCID: PMC4014287, PII: O113.033571, DOI: 10.1074/mcp.O113.033571, ISSN: 1535-9484.

Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Häuser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P. The binary protein-protein interaction landscape of Escherichia coli. Nat. Biotechnol [print-electronic]. 2014 Mar; 32(3): 285-90. PMID: 24561554, PMCID: PMC4123855, DOI: 10.1038/nbt.2831, ISSN: 1546-1696.

Blasche S, Wuchty S, Rajagopala SV, Uetz P. The protein interaction network of bacteriophage lambda with its host, Escherichia coli. J. Virol [print-electronic]. 2013 Dec; 87(23): 12745-55. PMID: 24049175, PMCID: PMC3838138, PII: JVI.02495-13, DOI: 10.1128/JVI.02495-13, ISSN: 1098-5514.

Memisevic V, Zavaljevski N, Pieper R, Rajagopala SV, Kwon K, Townsend K, Yu C, Yu X, DeShazer D, Reifman J, Wallqvist A. Novel Burkholderia mallei virulence factors linked to specific host-pathogen protein interactions. Mol. Cell Proteomics [print-electronic]. 2013 Nov; 12(11): 3036-51. PMID: 23800426, PMCID: PMC3820922, PII: M113.029041, DOI: 10.1074/mcp.M113.029041, ISSN: 1535-9484.

Rajagopala SV, Sikorski P, Caufield JH, Tovchigrechko A, Uetz P. Studying protein complexes by the yeast two-hybrid system. Methods [print-electronic]. 2012 Dec; 58(4): 392-9. PMID: 22841565, PMCID: PMC3517932, PII: S1046-2023(12)00171-5, DOI: 10.1016/j.ymeth.2012.07.015, ISSN: 1095-9130.

Häuser R, Stellberger T, Rajagopala SV, Uetz P. Array-based yeast two-hybrid screens: a practical guide. Methods Mol. Biol. 2012; 812: 21-38. PMID: 22218852, DOI: 10.1007/978-1-61779-455-1_2, ISSN: 1940-6029.

Häuser R, Stellberger T, Rajagopala SV, Uetz P. Matrix-based yeast two-hybrid screen strategies and comparison of systems. Methods Mol. Biol. 2012; 812: 1-20. PMID: 22218851, DOI: 10.1007/978-1-61779-455-1_1, ISSN: 1940-6029.

Rajagopala SV, Casjens S, Uetz P. The protein interaction map of bacteriophage lambda. BMC Microbiol. 2011 Sep 9/26/2011; 11: 213. PMID: 21943085, PMCID: PMC3224144, PII: 1471-2180-11-213, DOI: 10.1186/1471-2180-11-213, ISSN: 1471-2180.

Rajagopala SV, Uetz P. Analysis of protein-protein interactions using high-throughput yeast two-hybrid screens. Methods Mol. Biol. 2011; 781: 1-29. PMID: 21877274, DOI: 10.1007/978-1-61779-276-2_1, ISSN: 1940-6029.

Chen YC, Rajagopala SV, Stellberger T, Uetz P. Exhaustive benchmarking of the yeast two-hybrid system [letter]. Nat. Methods. 2010 Sep; 7(9): 667-8; author reply 668. PMID: 20805792, PII: nmeth0910-667, DOI: 10.1038/nmeth0910-667, ISSN: 1548-7105.

Rajagopala SV, Yamamoto N, Zweifel AE, Nakamichi T, Huang HK, Mendez-Rios JD, Franca-Koh J, Boorgula MP, Fujita K, Suzuki K, Hu JC, Wanner BL, Mori H, Uetz P. The Escherichia coli K-12 ORFeome: a resource for comparative molecular microbiology. BMC Genomics. 2010 Aug 8/11/2010; 11: 470. PMID: 20701780, PMCID: PMC3091666, PII: 1471-2164-11-470, DOI: 10.1186/1471-2164-11-470, ISSN: 1471-2164.

Rajagopala SV, Hughes KT, Uetz P. Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins. Proteomics. 2009 Dec; 9(23): 5296-302. PMID: 19834901, PMCID: PMC2818629, DOI: 10.1002/pmic.200900282, ISSN: 1615-9861.

Fossum E, Friedel CC, Rajagopala SV, Titz B, Baiker A, Schmidt T, Kraus T, Stellberger T, Rutenberg C, Suthram S, Bandyopadhyay S, Rose D, von Brunn A, Uhlmann M, Zeretzke C, Dong YA, Boulet H, Koegl M, Bailer SM, Koszinowski U, Ideker T, Uetz P, Zimmer R, Haas J. Evolutionarily conserved herpesviral protein interaction networks. PLoS Pathog [print-electronic]. 2009 Sep; 5(9): e1000570. PMID: 19730696, PMCID: PMC2731838, DOI: 10.1371/journal.ppat.1000570, ISSN: 1553-7374.

Rajagopala SV, Uetz P. Analysis of protein-protein interactions using array-based yeast two-hybrid screens. Methods Mol. Biol. 2009; 548: 223-45. PMID: 19521828, DOI: 10.1007/978-1-59745-540-4_13, ISSN: 1064-3745.

Rajagopala SV, Goll J, Gowda ND, Sunil KC, Titz B, Mukherjee A, Mary SS, Raviswaran N, Poojari CS, Ramachandra S, Shtivelband S, Blazie SM, Hofmann J, Uetz P. MPI-LIT: a literature-curated dataset of microbial binary protein--protein interactions. Bioinformatics [print-electronic]. 2008 Nov 11/15/2008; 24(22): 2622-7. PMID: 18786976, PMCID: PMC2720748, PII: btn481, DOI: 10.1093/bioinformatics/btn481, ISSN: 1367-4811.

Goll J, Rajagopala SV, Shiau SC, Wu H, Lamb BT, Uetz P. MPIDB: the microbial protein interaction database. Bioinformatics [print-electronic]. 2008 Aug 8/1/2008; 24(15): 1743-4. PMID: 18556668, PMCID: PMC2638870, PII: btn285, DOI: 10.1093/bioinformatics/btn285, ISSN: 1367-4811.

Titz B, Rajagopala SV, Goll J, Häuser R, McKevitt MT, Palzkill T, Uetz P. The binary protein interactome of Treponema pallidum--the syphilis spirochete. PLoS ONE. 2008 May 5/28/2008; 3(5): e2292. PMID: 18509523, PMCID: PMC2386257, DOI: 10.1371/journal.pone.0002292, ISSN: 1932-6203.

Rajagopala SV, Titz B, Goll J, Parrish JR, Wohlbold K, McKevitt MT, Palzkill T, Mori H, Finley RL, Uetz P. The protein network of bacterial motility. Mol. Syst. Biol [print-electronic]. 2007; 3: 128. PMID: 17667950, PMCID: PMC1943423, PII: msb4100166, DOI: 10.1038/msb4100166, ISSN: 1744-4292.

Titz B, Rajagopala SV, Ester C, Häuser R, Uetz P. Novel conserved assembly factor of the bacterial flagellum. J. Bacteriol [print-electronic]. 2006 Nov; 188(21): 7700-6. PMID: 16936039, PMCID: PMC1636259, PII: JB.00820-06, DOI: 10.1128/JB.00820-06, ISSN: 0021-9193.

Uetz P, Dong YA, Zeretzke C, Atzler C, Baiker A, Berger B, Rajagopala SV, Roupelieva M, Rose D, Fossum E, Haas J. Herpesviral protein networks and their interaction with the human proteome. Science [print-electronic]. 2006 Jan 1/13/2006; 311(5758): 239-42. PMID: 16339411, PII: 1116804, DOI: 10.1126/science.1116804, ISSN: 1095-9203.

Titz B, Thomas S, Rajagopala SV, Chiba T, Ito T, Uetz P. Transcriptional activators in yeast. Nucleic Acids Res [electronic-print]. 2006; 34(3): 955-67. PMID: 16464826, PMCID: PMC1361621, PII: 34/3/955, DOI: 10.1093/nar/gkj493, ISSN: 1362-4962.

Uetz P, Rajagopala SV, Dong YA, Haas J. From ORFeomes to protein interaction maps in viruses. Genome Res. 2004 Oct; 14(10B): 2029-33. PMID: 15489322, PII: 14/10b/2029, DOI: 10.1101/gr.2583304, ISSN: 1088-9051.